CN107661286A - A kind of anti-aging skin care product containing Chinese herbaceous peony stem cell extract - Google Patents
A kind of anti-aging skin care product containing Chinese herbaceous peony stem cell extract Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a kind of anti-apolexis composition, the composition of following parts by weight is included in the anti-apolexis composition：1.1~9 parts of 0.03~2 part of Chinese herbaceous peony stem cell extract, 1.2~4 parts of peony extract, 0.7~5 part of Saussurea involucrata extract, 1.3~8 parts of western blue flower extract and purslane extract.Meanwhile the invention also discloses the skin care item containing above-mentioned anti-apolexis composition.Skin care item in the present invention include extract, peony extract, Saussurea involucrata extract, western blue flower extract and the purslane extract of Chinese herbaceous peony stem cell, nutriment in these extracts is combined can effective delaying skin aging, brighten skin simultaneously, and, it is useful and harmless to skin without preservative.
Description
Technical field
The present invention relates to a kind of anti-aging skin care item, especially a kind of anti-aging shield containing Chinese herbaceous peony stem cell extract
Skin product.
Background technology
Skin aging is the skin senescence phenomenon as caused by natural cause or non-natural factor.Skin histology day after people's birth
Reach increasingly, function is gradually active, will start to degenerate when reaching certain age, and this degeneration is often unconsciously slow in people
Slow-motion row.The growth stage of skin histology typically ends at 25 years old or so, and it is " bent angle of skin " to have this phase of person, hereafter grows certainly
Carried out simultaneously with aging, Elastic fibers are gradually thicker.
Skin ageing phenomenon is mainly manifested in two aspects：
1st, skin histology fails.The thickness of skin has obvious change with the increase at age.During the epidermis of people 20 years old most
Thickness, later gradually it is thinning, to senescence phase stratum granulosum can atrophy to disappear, prickle cell's short survival.Epidermal cell nuclear fission increases
Add, therefore melanin also increases, so that the colour of skin of the elderly is mostly brownish black.Because aged cells are attached to horny layer of epidermis, make
Skin surface is hardened, and tarnishes.Corium is most thick at 30 years old, and later gradual change is thin and with atrophy.Subcutaneous fat is reduced, and by
Changed in elastic fibers and collagenous fibres and gradually lose skin elasticity and tension force, further cause cutis laxa to be produced with wrinkle
It is raw；
2nd, physiological function is low.Sebaceous glands, sweat gland deterioration, sweat exclude to reduce with sebum, and skin gradually loses former times
Day gloss and become drying.Blood circulation hypofunction is difficult to supplement the necessary nutrition of skin, therefore the elderly's skin wound is difficult
Healing.
On October 25th, 2010, Nature Biotechnology have delivered the research institute of the Unhwa Corp companies of South Korea
It is successfully separated first and has cultivated the paper of plant stem cell, and certification has been obtained to the identity of plant stem cell, plant does
The research of cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, it can not only be divided into autogenous cell, moreover it is possible to other any plant cells are divided into, are qualified " general-purpose cells ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Metabolism of the plant stem cell to raising skin is proved, improves microcirculation, tightening up and recovering skin elasticity has major application
Value.
The content of the invention
Based on this, one kind is provided and can effectively be delayed it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
The skin care item of aging.
To achieve the above object, the technical scheme taken of the present invention is：
A kind of anti-apolexis composition, the composition of following parts by weight is included in the anti-apolexis composition：Chinese herbaceous peony stem cell carries
Take 0.03~2 part of thing, 1.2~4 parts of peony extract, 0.7~5 part of Saussurea involucrata extract, 1.3~8 parts of western blue flower extract and horse
1.1~9 parts of sativa extract.
Wherein, Chinese herbaceous peony stem cell can play interference effect by epigenetic factor pair human skin cell, can be effective
The power of regeneration of skin progenitor cell is encouraged, hyperplasia fibrillar structure albumen, accelerates self more raw and reparation of skin；Modern study table
Bright, moutan bark has antibacterial, anti-inflammatory, antiallergy, antitumor, hemostasis, dissolving stasis blood, clearing heat and detoxicating, calm, analgesia, spasmolysis isoreactivity,
Monocyte phagocytic function can also be promoted, improve body specific immune function, increase immune organ weight.Using when should note
Meaning, the deficiency of blood have cold, and pregnant woman and menorrhalgia person use with caution.Peony contains astragalin, can also be used as medicine in addition to viewing and admiring, and is lived for menstruation regulating
Blood；Saussurea involucrata extract can accelerate the metabolism of skin, reduce wrinkle, skin is kept gloss, plentiful, anti-aging, and
There is the effect of good to facial freckle, chloasma hepaticum etc.；The nearest research of Japan is drawn a conclusion：The average nutritive value of western blue flower and
Prophylaxis effect is far beyond other vegetables, taking the first place；Purslane contain two abundant oxyethylamines, malic acid, glucose, calcium,
The nutriment such as phosphorus, iron and vitamin E, carrotene, vitamin B, vitamin C, purslane nutritionally have one it is prominent
Feature, the content of fatty acid of its ω -3 are higher than people and plant；The aliphatic acid of ω -3 can suppress absorption of the human body to the solid acid of courage, reduce
Serum cholesterol concentration, improve vessel wall elasticity.
Preferably, the mass ratio of the Chinese herbaceous peony stem cell extract, western blue flower extract and purslane extract be 0.1~
0.7:3:2~5.It is highly preferred that the mass ratio of the Chinese herbaceous peony stem cell extract, western blue flower extract and purslane extract is
0.3:3:4.The inventor itself asked has found through test of many times, when Chinese herbaceous peony stem cell extract, western blue flower extract in skin care item
Mass ratio with purslane extract is 0.1~0.7:3:When 2~5, the anti-aging effects of skin care item are preferable；When skin care item Chinese medicine
The mass ratio of stem cell extract, western blue flower extract and purslane extract is 0.3:3:When 4, the anti-aging effects of skin care item
More preferably.
Preferably, the mass ratio of the Chinese herbaceous peony stem cell extract and Saussurea involucrata extract is 0.07~0.5:2.More preferably
Ground, the mass ratio of the Chinese herbaceous peony stem cell extract and Saussurea involucrata extract is 0.22:2.Present inventor is through test of many times
It was found that when present inventor has found through test of many times, when Chinese herbaceous peony stem cell extract in skin care item and Saussurea involucrata extract
Mass ratio is 0.07~0.5:When 2, the whitening effect of skin care item is preferable；When Chinese herbaceous peony stem cell extract and saussurea involucrata carry in skin care item
The mass ratio for taking thing is 0.22:When 2, the whitening effect of skin care item is more preferably.
Preferably, the composition of following parts by weight is included in the anti-apolexis composition：0.3 part of Chinese herbaceous peony stem cell extract,
4 parts of 1.9 parts of peony extract, 2.7 parts of Saussurea involucrata extract, 3 parts of western blue flower extract and purslane extract, present invention
People has found through test of many times, when containing 0.3 part of Chinese herbaceous peony stem cell extract, 1.9 parts of peony extract, Saussurea involucrata extract in skin care
When 4 parts of 2.7 parts, 3 parts of western blue flower extract and purslane extract, the anti-aging effects and whitening effect of skin care item are all good.
As the second aspect of the invention, the invention provides a kind of anti-aging skin care product, the skin care item include upper
State composition.
Preferably, Chinese herbaceous peony stem cell extract, peony extract, Saussurea involucrata extract, the blue flower extraction in west in the skin care item
The quality percentage total amount of thing and purslane extract is 8.1~11%.Present inventor has found through test of many times, works as skin care
Chinese herbaceous peony stem cell extract in product, peony extract, Saussurea involucrata extract, the quality hundred of western blue flower extract and purslane extract
When point total amount is 8.1~11%, the anti-aging ability of skin care item is more preferably.
Preferably, the Chinese herbaceous peony stem cell extract is prepared by the following：
S1, Chinese herbaceous peony new life root or/and branch sterilization, after removing xylem and marrow, it is inoculated in inducing culture, induction training
Support and obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract.
Wherein, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of Chinese herbaceous peony stem cell is placed on into -170 DEG C, then
Melt at normal temperatures, so repeatedly so that the cell membrane of Chinese herbaceous peony stem cell crushes；This method can by the vital activity of stem cell with
Power of regeneration is assigned in skin care item, is absorbed via skin, can be remarkably promoted nutrition and be penetrated into and absorb, can effectively lift skin actively
Property, improve skin quality from cell aspect.
Preferably, the peony extract is prepared by the following：Peony is after high speed bead mill method broken wall, using water
Formulation obtains peony extract；
The Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken to be avenged after ultrasonication using water extraction
Lotus extract；
The western blue flower extract is prepared by the following：The blue flower in Shanxi is taken to be used after the micro-nano method broken wall of low temperature
Water extraction obtains western blue flower extract；
The purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains purslane extract.
Relative to prior art, beneficial effects of the present invention are：
Extract, peony extract, Saussurea involucrata extract, the Xi Lanhua that skin care item in the present invention include Chinese herbaceous peony stem cell are carried
Take thing and purslane extract, the nutriment in these extracts combine can effective delaying skin aging, simultaneously
Skin is brightened, also, is free of preservative, it is useful and harmless to skin.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Chinese herbaceous peony stem cell is extracted
1.1 parts of 0.03 part of thing, 4 parts of peony extract, 0.7 part of Saussurea involucrata extract, 8 parts of western blue flower extract and purslane extract.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 42
Part, 15 parts of butanediol, 51 parts of Arabic gum and 126 parts of water, wherein, Chinese herbaceous peony stem cell extract in skin care item, peony extract,
The quality percentage total amount of Saussurea involucrata extract, western blue flower extract and purslane extract is 6.6%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) Chinese herbaceous peony stem cell extract is prepared by the following：
S1, the sterilization of Chinese herbaceous peony new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) peony extract is prepared by the following：Peony is obtained after high speed bead mill method broken wall using water extraction
Peony extract；
Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken after ultrasonication, obtaining saussurea involucrata using water extraction carries
Take thing；
Western blue flower extract is prepared by the following：The blue flower in Shanxi is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains western blue flower extract；
Purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain purslane extract；
(3) by peony extract, Saussurea involucrata extract, western blue flower extract and purslane extract and Trivent OCG 42
Part, 15 parts of butanediol, 51 parts of Arabic gum and 126 parts of mixing of water, after 36.3 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Chinese herbaceous peony stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 2
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Chinese herbaceous peony stem cell is extracted
0.14 part of thing, 3.2 parts of peony extract, 1.5 parts of Saussurea involucrata extract, 5.3 parts of western blue flower extract and purslane extract 2.6
Part.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 31
Part, 14 parts of butanediol, 29 parts of Arabic gum and 87 parts of water, wherein, Chinese herbaceous peony stem cell extract in skin care item, peony extract,
The quality percentage total amount of Saussurea involucrata extract, western blue flower extract and purslane extract is 8.5%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) Chinese herbaceous peony stem cell extract is prepared by the following：
S1, the sterilization of Chinese herbaceous peony new life branch, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) peony extract is prepared by the following：Peony is obtained after high speed bead mill method broken wall using water extraction
Peony extract；
Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken after ultrasonication, obtaining saussurea involucrata using water extraction carries
Take thing；
Western blue flower extract is prepared by the following：The blue flower in Shanxi is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains western blue flower extract；
Purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain purslane extract；
(3) by peony extract, Saussurea involucrata extract, western blue flower extract and purslane extract and Trivent OCG 31
Part, 14 parts of butanediol, 29 parts of Arabic gum and 87 parts of mixing of water, after 36.8 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Chinese herbaceous peony stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 3
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Chinese herbaceous peony stem cell is extracted
4 parts of 0.3 part of thing, 1.9 parts of peony extract, 2.7 parts of Saussurea involucrata extract, 3 parts of western blue flower extract and purslane extract.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 21
Part, 8 parts of butanediol, 33 parts of Arabic gum and 71 parts of water, wherein, Chinese herbaceous peony stem cell extract, peony extract, snow in skin care item
The quality percentage total amount of lotus extract, western blue flower extract and purslane extract is 9.8%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) Chinese herbaceous peony stem cell extract is prepared by the following：
S1, the sterilization of Chinese herbaceous peony new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) peony extract is prepared by the following：Peony is obtained after high speed bead mill method broken wall using water extraction
Peony extract；
Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken after ultrasonication, obtaining saussurea involucrata using water extraction carries
Take thing；
Western blue flower extract is prepared by the following：The blue flower in Shanxi is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains western blue flower extract；
Purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain purslane extract；
(3) by peony extract, Saussurea involucrata extract, western blue flower extract and purslane extract and Trivent OCG 21
Part, 8 parts of butanediol, 33 parts of Arabic gum and 71 parts of mixing of water, after 37 degrees Celsius stir, are filtered to remove excessive moisture,
Lotion is obtained, then addition Chinese herbaceous peony stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 4
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Chinese herbaceous peony stem cell is extracted
6.8 parts of 1.1 parts of thing, 1.4 parts of peony extract, 4.1 parts of Saussurea involucrata extract, 1.7 parts of western blue flower extract and purslane extract.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 28
Part, 11 parts of butanediol, 33 parts of Arabic gum and 72 parts of water, wherein, Chinese herbaceous peony stem cell extract in skin care item, peony extract,
The quality percentage total amount of Saussurea involucrata extract, western blue flower extract and purslane extract is 11%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) Chinese herbaceous peony stem cell extract is prepared by the following：
S1, the sterilization of Chinese herbaceous peony new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) peony extract is prepared by the following：Peony is obtained after high speed bead mill method broken wall using water extraction
Peony extract；
Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken after ultrasonication, obtaining saussurea involucrata using water extraction carries
Take thing；
Western blue flower extract is prepared by the following：The blue flower in Shanxi is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains western blue flower extract；
Purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain purslane extract；
(3) by peony extract, Saussurea involucrata extract, western blue flower extract and purslane extract and Trivent OCG 28
Part, 11 parts of butanediol, 33 parts of Arabic gum and 72 parts of mixing of water, after 37.5 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Chinese herbaceous peony stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
Embodiment 5
A kind of embodiment of the anti-apolexis composition of the present invention, include the composition of following parts by weight：Chinese herbaceous peony stem cell is extracted
9 parts of 2 parts of thing, 1.2 parts of peony extract, 5 parts of Saussurea involucrata extract, 1.3 parts of western blue flower extract and purslane extract.
A kind of embodiment of the anti-aging skin care product of the present invention, includes above-mentioned anti-apolexis composition, Trivent OCG 55
Part, 17 parts of butanediol, 49 parts of Arabic gum and 118 parts of water, wherein, Chinese herbaceous peony stem cell extract in skin care item, peony extract,
The quality percentage total amount of Saussurea involucrata extract, western blue flower extract and purslane extract is 8.1%.
The preparation method of above-mentioned anti-aging skin care product comprises the following steps：
(1) Chinese herbaceous peony stem cell extract is prepared by the following：
S1, the sterilization of Chinese herbaceous peony new life root, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains shape
Into confluent monolayer cells；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell through the micro-nano method broken wall of low temperature, then by water extraction
Extract；
(2) peony extract is prepared by the following：Peony is obtained after high speed bead mill method broken wall using water extraction
Peony extract；
Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken after ultrasonication, obtaining saussurea involucrata using water extraction carries
Take thing；
Western blue flower extract is prepared by the following：The blue flower in Shanxi is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains western blue flower extract；
Purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain purslane extract；
(3) by peony extract, Saussurea involucrata extract, western blue flower extract and purslane extract and Trivent OCG 55
Part, 17 parts of butanediol, 49 parts of Arabic gum and 118 parts of mixing of water, after 38 degrees Celsius stir, are filtered to remove superfluous water
Point, lotion is obtained, then addition Chinese herbaceous peony stem cell extract, which is uniformly dispersed, produces a kind of anti-aging skin cream.
The anti-aging effects test of the skin care item of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream of control group 1~4, wherein, the skin care of control group 1~4
Frost and the face cream of embodiment 3 differ only in Chinese herbaceous peony stem cell extract, western blue flower extract and purslane extract
Mass ratio, the content of other compositions and the preparation method of face cream are same as Example 3 in face cream；The shield of control group 1~4
The mass ratio of Chinese herbaceous peony stem cell extract, western blue flower extract and purslane extract is 0.1 successively in skin frost:3:2；0.5：3：
3；0.6：3：4.5；0.7：3：5.
Method of testing：Nine groups of face cream altogether, every group of face cream chooses the women 15 of 35~55 years old respectively, after washing one's face,
Sooner or later each to smear face once, usage time is 80 days, using German CK companies skin elasticity tester MPA580 (using constant
Negative pressure 450mbar) the skin maximum tension amount (Uf) after using preceding and use is detected, calculate being averaged for skin elasticity increment rate
Value；
Test result：It is as shown in table 1 below；
The anti-aging effects of the face cream of the present invention of table 1
Interpretation of result：Understood from upper table 1 the elastic ability of the face cream increase skin of embodiment 3 and control group 1~4 compared with
It is good；Wherein, the elastic ability of the increase skin of the face cream of embodiment 3 is most strong.
The whitening effect that the skin care of the present invention of embodiment 7 is flat is tested
Test object：The face cream of embodiment 1~5 and the face cream of control group 1~4, wherein, the skin care of control group 1~4
The mass ratio for differing only in Chinese herbaceous peony stem cell extract and Saussurea involucrata extract of frost and the face cream of embodiment 3, in face cream
The content of other compositions and the preparation method of face cream are same as Example 3；Chinese herbaceous peony is dry thin in the face cream of control group 1~4
The mass ratio of born of the same parents' extract and Saussurea involucrata extract is 0.07 successively：2；0.13：2；0.37：20.5：2.
Method of testing：Nine groups of face cream altogether, every group of face cream chooses the women 25 of 30~55 years old respectively, after washing one's face,
Sooner or later each to smear face once, usage time is 4 months, is surveyed using German CK companies skin elasticity tester MPA580+MX18
Probe header (detection wavelength 568nm) successively detection calculates skin using before face cream and using face cream rear face melanin content
The average slip of skin melanin (takes facial 6 points).Test result：It is as shown in table 2 below；
The effect for dispelling melanin of the face cream of the present invention of table 2
Interpretation of result：As shown in upper table 2, the reduction skin of the face cream of embodiment 1~3 and the face cream of control group 1~4
The effect of melanin is preferable, and accordingly, Chinese herbaceous peony stem cell carries in the face cream of embodiment 1~3 and the face cream of control group 1~4
The mass ratio of thing and Saussurea involucrata extract is taken to all fall within 0.07~0.5：In the range of 2；Wherein, reduced with the face cream of embodiment 3
The ability of dermal melanin is best.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of anti-apolexis composition, it is characterised in that the composition of following parts by weight is included in the anti-apolexis composition：Chinese herbaceous peony
0.03~2 part of stem cell extract, 1.2~4 parts of peony extract, 0.7~5 part of Saussurea involucrata extract, western blue flower extract 1.3~
8 parts and 1.1~9 parts of purslane extract.
2. anti-apolexis composition according to claim 1, it is characterised in that the Chinese herbaceous peony stem cell extract, Xi Lanhua
The mass ratio of extract and purslane extract is 0.1~0.7:3:2~5.
3. anti-apolexis composition according to claim 2, it is characterised in that the Chinese herbaceous peony stem cell extract, Xi Lanhua
The mass ratio of extract and purslane extract is 0.3:3:4.
4. anti-apolexis composition according to claim 1, it is characterised in that the Chinese herbaceous peony stem cell extract and saussurea involucrata carry
The mass ratio for taking thing is 0.07~0.5:2.
5. anti-apolexis composition according to claim 4, it is characterised in that the Chinese herbaceous peony stem cell extract and saussurea involucrata carry
The mass ratio for taking thing is 0.22:2.
6. anti-apolexis composition according to claim 1, it is characterised in that comprising following heavy in the anti-apolexis composition
Measure the composition of part：0.3 part of Chinese herbaceous peony stem cell extract, 1.9 parts of peony extract, 2.7 parts of Saussurea involucrata extract, western blue flower extract
3 parts and 4 parts of purslane extract.
7. a kind of anti-aging skin care product, it is characterised in that the skin care item include the combination described in any one of claim 1~6
Thing.
8. anti-aging skin care product according to claim 7, it is characterised in that Chinese herbaceous peony stem cell is extracted in the skin care item
Thing, peony extract, Saussurea involucrata extract, the quality percentage total amount of western blue flower extract and purslane extract are 8.1~11%.
9. anti-aging skin care product according to claim 7, it is characterised in that the Chinese herbaceous peony stem cell extract passes through following
Method obtains：
S1, Chinese herbaceous peony new life root or/and branch sterilization, after removing xylem and marrow, inducing culture is inoculated in, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Chinese herbaceous peony stem cell；
S4, gained Chinese herbaceous peony stem cell obtain described Chinese herbaceous peony stem cell extraction through the micro-nano method broken wall of low temperature, then by water extraction
Thing.
10. anti-aging skin care product according to claim 7, it is characterised in that the peony extract is by the following method
Obtain：Peony obtains peony extract after high speed bead mill method broken wall using water extraction；
The Saussurea involucrata extract is prepared by the following：Lanatechead saussurea herb with flower is taken after ultrasonication, obtaining saussurea involucrata using water extraction carries
Take thing；
The western blue flower extract is prepared by the following：The blue flower in Shanxi is taken after the micro-nano method broken wall of low temperature, using water extraction
Method obtains western blue flower extract；
The purslane extract is prepared by the following：Purslane is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain purslane extract.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619075A (en) * | 2018-05-31 | 2018-10-09 | 北昊干细胞与再生医学研究院有限公司 | Anti-apolexis composition, cosmetics and preparation method thereof |
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| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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2017
- 2017-11-01 CN CN201711062650.9A patent/CN107661286A/en not_active Withdrawn
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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| 王建新等: "《化妆品植物原料大全》", 30 June 2012 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619075A (en) * | 2018-05-31 | 2018-10-09 | 北昊干细胞与再生医学研究院有限公司 | Anti-apolexis composition, cosmetics and preparation method thereof |
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Application publication date: 20180206 |