CN107616964A - A kind of Korean ginseng stem cell skin care composition and preparation method thereof - Google Patents
A kind of Korean ginseng stem cell skin care composition and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of Korean ginseng stem cell skin care composition and preparation method thereof, the Korean ginseng stem cell skin care composition, it is made up of the component of following parts by weight：Part A：3~10 parts of 0.5~6 part of Korean ginseng stem cell extract, 5~10 parts of climbing fig syconium extract and echinacea extract；Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；C portion：0.5~1.5 part of 2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, 0.5~2 part of polyoxyethylene stearyl base ether and glycerin monostearate.Korean ginseng stem cell extract has powerful anti-oxidant and activity of fighting against senium in the Korean ginseng stem cell skin care composition of the present invention, effectively facilitates differentiation of self and the metabolism of skin cell, by increasing capacitance it is possible to increase skin elasticity；Climbing fig syconium extract and echinacea extract all have the effect of good anti-aging, and three's combination can cooperate with anti-aging, can reduce skin water loss, improve skin elasticity, the effect with comprehensive anti-aging.
Description
Technical field
The present invention relates to skin care field, and in particular to a kind of Korean ginseng stem cell skin care composition and preparation method thereof.
Background technology
Skin care be it is important in global beauty and personal care industries segment market, and anti-aging skin care product is standby wherein
It is concerned.Since ancient times, the mankind are directed to finding the active material with anti-aging effects always.The reason for causing skin senescence
Mainly have：1) ultraviolet irradiation causes collagen to be lost in, so as to accelerate skin aging；2) attack of oxygen radical is led in vivo
Cause skin injury；3) the fibroblast natural aging in skin.Although skin senescence is the natural physiological phenomenon of human body,
Environmental factor can cause skin senescence to aggravate, and some safe anti-aging materials are added in skin care item, can be with delaying skin
Aging.
It is mainly crease-resistant to skin progress by adding hormone and plant extracts etc. in anti-aging skin care product on the market
Processing, but the abuse of hormone easily causes side effect, plant extracts Small side effects, but single active ingredient to be often difficult to reach
To preferable effect, anti-aging short run effect unobvious.
Plant stem cell is undifferentiated cell in plant, is the source and signals-modulating center to grow, has
Very strong self-renewing and lasting splitting ability.Research shows that certain plants stem cell has powerful anti-oxidant and anti-aging
Activity, a new method can be provided as the activator of human stem cell for mankind's anti-aging.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of Korean ginseng stem cell skin care composition, tool
There is the effect of comprehensive anti-aging.
It is another object of the present invention to provide a kind of preparation method of Korean ginseng stem cell skin care composition.
To achieve the above object, the technical scheme that the present invention takes is as follows：
A kind of Korean ginseng stem cell skin care composition, is made up of the component of following parts by weight：
Part A：0.5~6 part of Korean ginseng stem cell extract, 5~10 parts of climbing fig syconium extract and Echinacea purpurea extraction
3~10 parts of thing；
Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；
C portion：2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, polyoxyethylene stearyl base ether
0.5~2 part and 0.5~1.5 part of glycerin monostearate.
Above-mentioned Korean ginseng stem cell extract has powerful anti-oxidant and activity of fighting against senium, effectively facilitates skin cell
Differentiation of self and metabolism, by increasing capacitance it is possible to increase skin elasticity；Climbing fig syconium extract and echinacea extract all have good
Anti-aging the effect of, three's combination can cooperate with anti-aging, reduce skin water loss, improve skin elasticity, have comprehensive
The effect of anti-aging.
As the preferred embodiment of Korean ginseng stem cell skin care composition of the present invention, by following parts by weight
Component is made：
Part A：2~5 parts of Korean ginseng stem cell extract, 7~9 parts of climbing fig syconium extract and echinacea extract 5
~8 parts；
Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；
C portion：2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, polyoxyethylene stearyl base ether
0.5~2 part and 0.5~1.5 part of glycerin monostearate.
As the preferred embodiment of Korean ginseng stem cell skin care composition of the present invention, by following parts by weight
Component is made：
Part A：6 parts of 4 parts of Korean ginseng stem cell extract, 8 parts of climbing fig syconium extract and echinacea extract；
Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；
C portion：2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, polyoxyethylene stearyl base ether
0.5~2 part and 0.5~1.5 part of glycerin monostearate.
As the preferred embodiment of Korean ginseng stem cell skin care composition of the present invention, the Korean ginseng stem cell
For Korean ginseng forming layer stem cell.
As the preferred embodiment of Korean ginseng stem cell skin care composition of the present invention, described Korean ginseng is dry thin
Born of the same parents' extract is the Korean ginseng stem cell media and the lysate of Korean ginseng stem cell after culture Korean ginseng stem cell.
The present invention also provides a kind of preparation method of described Korean ginseng stem cell skin care composition, comprises the following steps：
(1) the Korean ginseng stem cell media after culture Korean ginseng stem cell and the lysate of Korean ginseng stem cell are freezed
Dry, obtain Korean ginseng stem cell extract；
(2) climbing fig syconium extract is prepared；
(3) each component raw material is weighed in proportion；
(4) part B and C portion are heated with stirring to 80 DEG C~85 DEG C respectively, dissolving is complete；
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
As the preferred embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, the step
Suddenly in (2), climbing fig syconium extract is prepared using ultrasonic wave added extraction.
As the preferred embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, the training
Base is supported before freeze-drying, through ultrafiltration membrance filter, concentrates filtrate, removes phenol red in culture medium, the activity in reservation culture medium
Composition.
Compared with prior art, the invention has the advantages that：
Korean ginseng stem cell extract has powerful anti-oxidant and activity of fighting against senium, effectively facilitates skin cell self
Differentiation and metabolism, by increasing capacitance it is possible to increase skin elasticity；Climbing fig syconium extract and echinacea extract all have good resist
The effect of aging, three's combination can cooperate with anti-aging, can reduce skin water loss, improve skin elasticity, have comprehensive
The effect of anti-aging.
Brief description of the drawings
Fig. 1 is keratoderma moisture determination result figure.
Fig. 2 is coefficient of skin elasticity measurement result.
Embodiment
For the object, technical solutions and advantages of the present invention are better described, below in conjunction with specific embodiment to the present invention
Further illustrate.It will be appreciated by those skilled in the art that specific embodiment described herein is only to explain the present invention, not
For limiting the present invention.
Test method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
The material that uses, reagent etc., unless otherwise specified, are commercially obtained.
Embodiment 1
A kind of embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, Korean ginseng described in the present embodiment
The preparation method of stem cell skin care composition is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of Korean ginseng forming layer stem cell is dry
It is dry, obtain Korean ginseng stem cell extract.
(2) climbing fig syconium extract is prepared
The preparation of climbing fig syconium extract：Climbing fig syconium uses petroleum ether extraction, and residual residue is dried at 50 DEG C
To constant weight, the residue of drying is crushed, 200 mesh sieves is crossed and obtains climbing fig syconium powder, climbing fig syconium powder is surpassed by ethanol
After sound extraction, 10min is centrifuged under 5000r/min speed, takes supernatant rotation to be evaporated, obtains the climbing fig syconium extraction
Thing.
Echinacea extract is purchased from Xi'an Watson Biology Technology Co., Ltd..
(3) each component raw material is weighed in proportion.
Part A：3 parts of 0.5 part of Korean ginseng stem cell extract, 5 parts of climbing fig syconium extract and echinacea extract；
Part B：20 parts of 0.1 part of xanthans, 1 part of glycerine and water；
C portion：3 parts of DPG, 0.4 part of monostearate macrogol ester, 0.5 part of polyoxyethylene stearyl base ether and
0.6 part of glycerin monostearate.
(4) part B and C portion are heated with stirring into 80 DEG C respectively extremely to dissolve completely.
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
Embodiment 2
A kind of embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, Korean ginseng described in the present embodiment
The preparation method of stem cell skin care composition is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of Korean ginseng forming layer stem cell is dry
It is dry, obtain Korean ginseng stem cell extract.
(2) climbing fig syconium extract is prepared
The preparation of climbing fig syconium extract：Climbing fig syconium uses petroleum ether extraction, and residual residue is dried at 50 DEG C
To constant weight, the residue of drying is crushed, 200 mesh sieves is crossed and obtains climbing fig syconium powder, climbing fig syconium powder is surpassed by ethanol
After sound extraction, 10min is centrifuged under 5000r/min speed, takes supernatant rotation to be evaporated, obtains the climbing fig syconium extraction
Thing.
Echinacea extract is purchased from Xi'an Watson Biology Technology Co., Ltd..
(3) each component raw material is weighed in proportion.
Part A：7 parts of 5 parts of Korean ginseng stem cell extract, 8 parts of climbing fig syconium extract and echinacea extract；
Part B：40 parts of 0.5 part of xanthans, 2 parts of glycerine and water；
C portion：3 parts of DPG, 0.7 part of monostearate macrogol ester, 1.5 parts of polyoxyethylene stearyl base ether and
1.4 parts of glycerin monostearate.
(4) part B and C portion are heated with stirring into 80 DEG C respectively extremely to dissolve completely.
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
Embodiment 3
A kind of embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, Korean ginseng described in the present embodiment
The preparation method of stem cell skin care composition is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of Korean ginseng forming layer stem cell is dry
It is dry, obtain Korean ginseng stem cell extract.
(2) climbing fig syconium extract is prepared
The preparation of climbing fig syconium extract：Climbing fig syconium uses petroleum ether extraction, and residual residue is dried at 50 DEG C
To constant weight, the residue of drying is crushed, 200 mesh sieves is crossed and obtains climbing fig syconium powder, climbing fig syconium powder is surpassed by ethanol
After sound extraction, 10min is centrifuged under 5000r/min speed, takes supernatant rotation to be evaporated, obtains the climbing fig syconium extraction
Thing.
Echinacea extract is purchased from Xi'an Watson Biology Technology Co., Ltd..
(3) each component raw material is weighed in proportion.
Part A：5 parts of 2 parts of Korean ginseng stem cell extract, 7 parts of climbing fig syconium extract and echinacea extract；
Part B：45 parts of 1 part of xanthans, 3 parts of glycerine and water；
C portion：4 parts of DPG, 0.6 part of monostearate macrogol ester, 2 parts of polyoxyethylene stearyl base ether and list
0.5 part of tristerin.
(4) part B and C portion are heated with stirring into 80 DEG C respectively extremely to dissolve completely.
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
Embodiment 4
A kind of embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, Korean ginseng described in the present embodiment
The preparation method of stem cell skin care composition is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of Korean ginseng forming layer stem cell is dry
It is dry, obtain Korean ginseng stem cell extract.
(2) climbing fig syconium extract is prepared
The preparation of climbing fig syconium extract：Climbing fig syconium uses petroleum ether extraction, and residual residue is dried at 50 DEG C
To constant weight, the residue of drying is crushed, 200 mesh sieves is crossed and obtains climbing fig syconium powder, climbing fig syconium powder is surpassed by ethanol
After sound extraction, 10min is centrifuged under 5000r/min speed, takes supernatant rotation to be evaporated, obtains the climbing fig syconium extraction
Thing.
Echinacea extract is purchased from Xi'an Watson Biology Technology Co., Ltd..
(3) each component raw material is weighed in proportion.
Part A：8 parts of 5 parts of Korean ginseng stem cell extract, 9 parts of climbing fig syconium extract and echinacea extract；
Part B：50 parts of 1 part of xanthans, 2 parts of glycerine and water；
C portion：3 parts of DPG, 00.5 part of monostearate macrogol ester, 2 parts of polyoxyethylene stearyl base ether and list
1 part of tristerin.
(4) part B and C portion are heated with stirring into 80 DEG C respectively extremely to dissolve completely.
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
Embodiment 5
A kind of embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, Korean ginseng described in the present embodiment
The preparation method of stem cell skin care composition is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of Korean ginseng forming layer stem cell is dry
It is dry, obtain Korean ginseng stem cell extract.
(2) climbing fig syconium extract is prepared
The preparation of climbing fig syconium extract：Climbing fig syconium uses petroleum ether extraction, and residual residue is dried at 50 DEG C
To constant weight, the residue of drying is crushed, 200 mesh sieves is crossed and obtains climbing fig syconium powder, climbing fig syconium powder is surpassed by ethanol
After sound extraction, 10min is centrifuged under 5000r/min speed, takes supernatant rotation to be evaporated, obtains the climbing fig syconium extraction
Thing.
Echinacea extract is purchased from Xi'an Watson Biology Technology Co., Ltd..
(3) each component raw material is weighed in proportion.
Part A：6 parts of 4 parts of Korean ginseng stem cell extract, 8 parts of climbing fig syconium extract and echinacea extract；
Part B：30 parts of 0.4 part of xanthans, 3 parts of glycerine and water；
C portion：4 parts of DPG, 0.8 part of monostearate macrogol ester, 2 parts of polyoxyethylene stearyl base ether and list
1 part of tristerin.
(4) part B and C portion are heated with stirring into 80 DEG C respectively extremely to dissolve completely.
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
Embodiment 6
A kind of embodiment of the preparation method of Korean ginseng stem cell skin care composition of the present invention, anti-wrinkle described in the present embodiment are protected
The preparation method of skin product is：
(1) Korean ginseng stem cell extract is prepared
1) separation and culture of Korean ginseng forming layer stem cell
By the root tuber of Korean ginseng after disinfecting, the semicircle section including forming layer is cut；
Semi-circular section is placed on WPM culture mediums, dark culturing at 25 DEG C；
The outer paraphyte of forming layer had significant proliferation is taken out after two weeks, forming layer stem cell is separated and is transferred to squamous subculture
Cultivated in base, subculture once, obtains Korean ginseng forming layer stem cell every two weeks；
2) culture medium after culture Korean ginseng forming layer stem cell is collected, through ultrafiltration membrance filter, filtrate is concentrated, removes culture
It is phenol red in base, retain the active component in culture medium；Culture medium and the freezing of the lysate of Korean ginseng forming layer stem cell is dry
It is dry, obtain Korean ginseng stem cell extract.
(2) climbing fig syconium extract is prepared
The preparation of climbing fig syconium extract：Climbing fig syconium uses petroleum ether extraction, and residual residue is dried at 50 DEG C
To constant weight, the residue of drying is crushed, 200 mesh sieves is crossed and obtains climbing fig syconium powder, climbing fig syconium powder is surpassed by ethanol
After sound extraction, 10min is centrifuged under 5000r/min speed, takes supernatant rotation to be evaporated, obtains the climbing fig syconium extraction
Thing.
Echinacea extract is purchased from Xi'an Watson Biology Technology Co., Ltd..
(3) each component raw material is weighed in proportion.
Part A：10 parts of 6 parts of Korean ginseng stem cell extract, 10 parts of climbing fig syconium extract and echinacea extract；
Part B：50 parts of 1 part of xanthans, 2 parts of glycerine and water；
C portion：4 parts of DPG, 0.8 part of monostearate macrogol ester, 0.5 part of polyoxyethylene stearyl base ether and
1.4 parts of glycerin monostearate.
(4) part B and C portion are heated with stirring into 80 DEG C respectively extremely to dissolve completely.
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
Korean ginseng stem cell skin care composition efficacy assessments
Using the Korean ginseng stem cell skin care composition prepared by embodiment 1~6 as experimental group, with the institute of comparative example 1~3
The skin care compositions of preparation as a control group, carries out related efficacy test.The A phases of the skin care compositions of comparative example by table 1 group
It is grouped into, other components and preparation method are the same as embodiment 5.
Table 1
(1) superoxide anion removes measure
It is 50mg/mL's that skin care compositions prepared by embodiment 1~6 and comparative example 1~3 is configured into concentration respectively
The aqueous solution.0.1mL is taken to be separately added into NaOHs of the 1mL containing 557 μm of ol/L 16 mmol/L Tris-HCl (pH=8.0), 1mL contains
45 μm of ol/L PMS 16mmol/L Tris-HCl (pH 8.0), and the 16mmol/L Tris- of the NBT containing 108 μm of ol/L
HCl (pH=8.0).25 DEG C of min of warm bath 5, light absorption value is surveyed under ELIASA 560nm, and superoxide anion is calculated according to formula (1)
Clearance rate, as a result as shown in table 2.
Superoxide anion clearance rate=(1- sample cells A560/ control tube A560) × 100% (1)
Table 2
Superoxide anion is a kind of living radical caused by organism metabolism process, has close connection with skin aging
System.As seen from the results in Table 2, the superoxide anion clearance rate of skin care compositions prepared by embodiment shows apparently higher than comparative example
There is synergy, the present invention between Korean ginseng stem cell extract, climbing fig syconium extract and echinacea extract three
The skin care compositions of preparation has good collaboration antioxidation, is advantageous to delay skin aging.
(2) Hydroxyl radical-scavenging determines
It is 50mg/mL's that skin care compositions prepared by embodiment 1~6 and comparative example 1~3 is configured into concentration respectively
The aqueous solution.0.1mL is taken to be separately added into 0.6mL reaction buffers (deoxyribose containing 2.67mmol/L and 0.13mmol/L EDTA
0.2mol/L pH=7.4 phosphate buffer), 0.4mmol/L ferrous sulfate 0.2mL, 2.0mmol/L ascorbic acid
0.05mL, and 20mmol/L H2O20.05 mL, 37 DEG C of warm bath 15min, added after taking-up 1% thiobarbituricacidα- 1mL and
2% trichloroacetic acid 1mL, boiling water bath 15min, in cooled on ice after taking-up, light absorption value is surveyed under ELIASA 532nm, according to formula
(2) Scavenging action to hydroxyl free radical is calculated, as a result as shown in table 3.
Scavenging action to hydroxyl free radical=(1- sample cells A532/ control tube A532) × 100% (2)
Table 3
Hydroxy radical is the principal element for causing biologic artifact peroxide injury as active oxygen radical.Can by table 3
Know, the Scavenging action to hydroxyl free radical of skin care compositions prepared by embodiment shows that Korean ginseng stem cell is extracted apparently higher than comparative example
There is synergy, skin care compositions prepared by the present invention between thing, climbing fig syconium extract and echinacea extract three
The scavenging action of hydroxy radical be better than control group, Korean ginseng stem cell extract, climbing fig syconium extract and Echinacea purpurea carry
Take thing that there is good collaboration antioxidation, be advantageous to skin anti-aging.
(3) keratoderma moisture determination
Skin care compositions made from embodiment 1~6 and comparative example 1~3, each group select 30 ages 35~55 respectively
The female volunteers in year, skin care compositions is being smeared on the face after volunteer's face cleaning, is being used continuously 6 months, passes through skinanalysis apparatus
(MC750, German CK) determines water content of stratum corneum, and test result is as shown in Figure 1.
Have in keratoderma with reference to water and the two kinds of moisture of free water, moisture can influence cuticula reclaimed water and
The formation of grease sebum film, sebum film can prevent moisture excessive evaporation, softening skin, have important meaning to the anti-aging of skin
Justice.As shown in Figure 1, after Korean ginseng stem cell skin care composition of the volunteer using embodiment preparation, keratoderma water content
Comparative example is substantially better than, shows Korean ginseng stem cell extract, climbing fig syconium extract and echinacea extract triplicity
The water content for improving skin can be cooperateed with, reduces skin water loss.
(4) skin elasticity is tested
Skin care compositions made from embodiment 1~6 and comparative example 1~3, every group was selected 30 ages at 35~55 years old respectively
Female volunteers, volunteer is daily early, is smearing skin care compositions on the face after late face cleaning, is used continuously 6 months, passes through skin
Skin tester (MC750, German CK) determines skin elasticity, and test result is as shown in Figure 2.
The numerical value of coefficient of skin elasticity is bigger, and skin elasticity is better.As shown in Figure 2, volunteer is prepared using embodiment
After Korean ginseng stem cell skin care composition, coefficient of skin elasticity is hidden apparently higher than comparative example, Korean ginseng stem cell extract, climbing fig
Head berry extract and echinacea extract have good synergistic oxidation and moisturizing moisturizing effect, are advantageous to improve the bullet of skin
Property.
Result above shows that Korean ginseng stem cell skin care composition of the invention has good collaboration antioxidant effect,
With good moisture retention, skin water loss can be reduced, skin elasticity is improved, there are good anti-aging effects.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (8)
1. a kind of Korean ginseng stem cell skin care composition, it is characterised in that be made up of the component of following parts by weight：
Part A：0.5~6 part of Korean ginseng stem cell extract, 5~10 parts of climbing fig syconium extract and echinacea extract 3~
10 parts；
Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；
C portion：2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, polyoxyethylene stearyl base ether 0.5
~2 parts and 0.5~1.5 part of glycerin monostearate.
2. Korean ginseng stem cell skin care composition according to claim 1, it is characterised in that by the group of following parts by weight
Divide and be made：
Part A：2~5 parts of Korean ginseng stem cell extract, 7~9 parts of climbing fig syconium extract and echinacea extract 5~8
Part；
Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；
C portion：2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, polyoxyethylene stearyl base ether 0.5
~2 parts and 0.5~1.5 part of glycerin monostearate.
3. Korean ginseng stem cell skin care composition according to claim 1, it is characterised in that by the group of following parts by weight
Divide and be made：
Part A：6 parts of 4 parts of Korean ginseng stem cell extract, 8 parts of climbing fig syconium extract and echinacea extract；
Part B：20~50 parts of 0.1~1 part of xanthans, 1~3 part of glycerine and water；
C portion：2~4 parts of DPG, 0.25~0.8 part of monostearate macrogol ester, polyoxyethylene stearyl base ether 0.5
~2 parts and 0.5~1.5 part of glycerin monostearate.
4. Korean ginseng stem cell skin care composition according to claim 1, it is characterised in that the Korean ginseng stem cell is
Korean ginseng forming layer stem cell.
5. Korean ginseng stem cell skin care composition according to claim 1, it is characterised in that described Korean ginseng stem cell
Extract is the Korean ginseng stem cell media and the lysate of Korean ginseng stem cell after culture Korean ginseng stem cell.
6. the preparation method of the Korean ginseng stem cell skin care composition according to any one of Claims 1 to 5, its feature exist
In comprising the following steps：
(1) the Korean ginseng stem cell media after culture Korean ginseng stem cell and the lysate of Korean ginseng stem cell are freezed dry
It is dry, obtain Korean ginseng stem cell extract；
(2) climbing fig syconium extract is prepared；
(3) each component raw material is weighed in proportion；
(4) part B and C portion are heated with stirring to 80 DEG C~85 DEG C respectively, dissolving is complete；
(5) C portion is added in part B under stirring, 45 DEG C is cooled to after emulsifying is uniform, then by part A
Add, be uniformly mixing to obtain the Korean ginseng stem cell skin care composition.
7. the preparation method of Korean ginseng stem cell skin care composition according to claim 6, it is characterised in that the step
(2) in, climbing fig syconium extract is prepared using ultrasonic wave added extraction.
8. the preparation method of Korean ginseng stem cell skin care composition according to claim 6, it is characterised in that the culture
Base is before freeze-drying, through ultrafiltration membrance filter, concentrates filtrate, remove it is phenol red in culture medium, retain activity in culture medium into
Point.
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Citations (3)
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|---|---|---|---|---|
| CN102159182A (en) * | 2008-06-13 | 2011-08-17 | 云火公司 | Composition for anti-aging or antioxidation containing plant stem cell lines derived from cambium of panax ginseng including wild ginseng and ginseng as active components |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
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- 2017-09-15 CN CN201710838457.3A patent/CN107616964A/en not_active Withdrawn
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|---|---|---|---|---|
| CN102159182A (en) * | 2008-06-13 | 2011-08-17 | 云火公司 | Composition for anti-aging or antioxidation containing plant stem cell lines derived from cambium of panax ginseng including wild ginseng and ginseng as active components |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
| CN105816807A (en) * | 2016-04-28 | 2016-08-03 | 四川力久知识产权服务有限公司 | Anti-aging traditional Chinese medicine composition containing scrophularia ningpoensis for cosmetics |
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