CN107607473B - Simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging device and method - Google Patents
Simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging device and method Download PDFInfo
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Abstract
The invention discloses a simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging device and a method, wherein in the device, a computer processing system controls the on-off of a photoacoustic signal excitation system, receives data processed by a photoacoustic signal preprocessing system, processes the data, then carries out reconstruction imaging and controls the operation of a three-dimensional scanning system; the photoacoustic signal excitation system shapes pulse laser into a linear light source and then focuses a plurality of light focuses by using a micro-lens array, and the plurality of light focuses simultaneously excite a sample to generate a multi-source photoacoustic signal; the photoacoustic signal receiving system receives multi-source photoacoustic signals in parallel; the photoacoustic signal preprocessing system amplifies and filters the multi-source photoacoustic signals and sends the signals to the computer processing system; the sample to be imaged is placed in a water tank, and the three-dimensional scanning system is used for adjusting the spatial positions of the water tank and the photoacoustic signal excitation system. The invention can realize rapid and large-scale photoacoustic three-dimensional imaging by utilizing the spatial layout of the micro-lens array and the linear acoustic detection array.
Description
Technical Field
The invention relates to the field of photoacoustic imaging research, in particular to a photoacoustic three-dimensional imaging device and method capable of simultaneously carrying out multipoint excitation and matching on received light.
Background
Photoacoustic imaging is a non-invasive imaging technique combining optical imaging and ultrasound imaging, which has the characteristics of high contrast, high resolution of optical imaging and high penetration of ultrasound imaging. The photoacoustic imaging is based on the fundamental principle of photo-generated sound, when short-pulse laser light is irradiated on biological tissue, the biological tissue absorbs pulse energy and expands rapidly to generate ultrasound, namely photoacoustic signals, and images reflecting biological tissue structure and function information can be reconstructed according to the photoacoustic signals. Photoacoustic imaging overcomes some of the disadvantages of conventional imaging approaches, such as: compared with Optical Coherence Tomography (OCT), the measurement depth of OCT is limited to be a shallow layer in millimeter order due to strong scattering of tissue optical property, and the photoacoustic imaging technology can reach centimeter order; compared with pure ultrasonic imaging, the contrast of ultrasonic imaging in a region with a small acoustic impedance difference is low, and photoacoustic imaging utilizes the absorption difference of different tissues to reconstruct an image with high contrast, so that the rapid development of photoacoustic imaging has a promoting effect on the development of the field of biomedical imaging.
For photoacoustic imaging, it is important to improve imaging speed to monitor dynamic physiological processes in real time, but limited by the repetition frequency of pulse laser and the speed of mechanical scanning, most of the currently adopted imaging modes of single optical focus and single acoustic focus are difficult to realize high-speed photoacoustic three-dimensional imaging. The patent application with the application number of 201010139117.X discloses a rapid three-dimensional photoacoustic imaging system and method based on an area array ultrasonic detector, the invention establishes a non-scanning photoacoustic imaging system based on the area array detector to realize rapid three-dimensional photoacoustic imaging, but the system has the defect that an excitation light source is non-focused laser, so the resolution of the system is determined by acoustic resolution, but is limited by the array element size and the array element interval of the area array ultrasonic detector, and the system is difficult to realize high-resolution microscopic photoacoustic imaging. The patent application No. 201110171027.3 discloses a fast photoacoustic three-dimensional imaging device, which avoids the use of a mechanical scanning device by controlling the on-off of each microlens in an optical mask through a programmable logic array module, but the utilization rate of a laser source is extremely low because most microlenses are in an off state in the working state of the device, complicated circuit control is required for excitation of the light source and acquisition of photoacoustic signals, and the positions and time sequences of excitation and acquisition are strictly unified, so that the whole hardware and program are complicated and inconvenient for practical application.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a photoacoustic three-dimensional imaging device and method capable of simultaneously carrying out multipoint excitation and matching on received photoacoustic, wherein the device reasonably utilizes the spatial layout of a micro-lens array and a linear acoustic detection array, realizes efficient photoacoustic excitation and receiving, and can realize rapid photoacoustic three-dimensional imaging in a large range.
The purpose of the invention is realized by the following technical scheme: a simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging device comprises a computer processing system, a photoacoustic signal excitation system, a photoacoustic signal receiving system, a photoacoustic signal preprocessing system and a three-dimensional scanning system, wherein:
the computer processing system controls the on-off of the photoacoustic signal excitation system, receives data processed by the photoacoustic signal preprocessing system, processes the data, reconstructs and images the data and controls the operation of the three-dimensional scanning system;
the photoacoustic signal excitation system firstly shapes pulse laser into a linear light source, then a plurality of light focuses are focused by utilizing a micro-lens array in the photoacoustic signal excitation system, multi-focus linear excitation is realized, and a plurality of light focuses simultaneously excite a sample to generate a multi-source photoacoustic signal;
the photoacoustic signal receiving system receives the multi-source photoacoustic signals in parallel;
the photoacoustic signal preprocessing system amplifies and filters a multi-source photoacoustic signal and sends the signal to a computer processing system through a multi-channel signal acquisition system;
the sample to be imaged is placed in a water tank, and the three-dimensional scanning system is used for adjusting the spatial positions of the water tank and the photoacoustic signal excitation system.
Preferably, the photoacoustic signal excitation system comprises a nanosecond pulse laser, a coupling light path, a linear laser generating lens and a micro lens array, wherein the coupling light path couples pulse laser emitted by the nanosecond pulse laser into an optical fiber for transmission and collimation to obtain parallel light beams, the linear laser generating lens converts the parallel light beams into a linear laser with uniform energy distribution, the micro lens array is fixed under the linear laser generating lens, a single-row micro lens unit array is adopted, and the linear laser generates a row of light focus after being focused by the micro lens array.
Furthermore, the coupling light path comprises an optical fiber coupler, a single-mode optical fiber and an optical fiber collimator, the pulse laser emitted by the nanosecond pulse laser couples the laser into the single-mode optical fiber through the optical fiber coupler, the pulse laser is transmitted to the optical fiber collimator through the single-mode optical fiber, and the optical fiber collimator and the linear laser generating lens are coaxially arranged. With optical elements, the coupling efficiency can be greater than 80%.
Furthermore, the single-mode fiber output end is connected with the fiber collimator through an FC/PC standard connector.
Furthermore, the linear laser generating lens adopts a Powell prism. The Bawell prism has stronger capability of converting parallel light beams into linear light sources with uniform energy distribution.
Furthermore, the microlens array is fused quartz lenses arranged in a square grid, only one row of microlens units are utilized after the optical custom cutting, and the shape of the microlens is round or square.
Furthermore, the micro lens array is plated with an antireflection film with the wavelength of 400-900 nm. The light transmittance is more than 99%, and linear laser can generate a focus of a light spot size limited by a near diffraction limit after being focused by the micro-lens array.
Preferably, the linear laser generating lens and the micro lens array are both bonded on an optical stainless steel lens base through epoxy resin glue.
Preferably, the photoacoustic signal receiving system adopts a splayed linear acoustic detection array, the acoustic detection array is composed of two sub-ultrasonic transducer arrays which are in mirror symmetry distribution, each sub-ultrasonic transducer array is composed of a plurality of acoustic detection array elements, each optical focus and two acoustic detection array elements which are geometrically corresponding to two sides form an optical/acoustic excitation receiving matching unit, and the splayed linear acoustic detection array collects excited multi-source photoacoustic signals through a phased array technology.
Furthermore, the two sub ultrasonic transducer arrays are arranged on two sides of the micro lens array at a certain inclination angle, and a line of light focus focused by the micro lens array passes through the middle part of the two sub ultrasonic transducer arrays completely and irradiates the surface of the sample. After the optical focus excites the sample to generate the photoacoustic signal, the sub-ultrasonic transducer arrays on the two sides are not shielded by the micro-lens array in space, and the transducer can completely receive the photoacoustic signal generated by the sample.
Preferably, the photoacoustic signal preprocessing system comprises a three-level multi-channel signal amplification module, a band-pass filter and a multi-channel signal acquisition system which are sequentially connected.
Preferably, the three-dimensional scanning system comprises a three-dimensional scanning platform, a three-dimensional scanning platform driver and a scanning controller, the scanning controller is connected with a computer processing system, and the computer processing system controls the scanning speed and the scanning range of the three-dimensional scanning platform.
Furthermore, the three-dimensional scanning platform comprises three one-dimensional ultrasonic motors, is provided with a grating ruler and adopts closed-loop control.
Furthermore, the shell of the three-dimensional scanning platform, the ground wire of the three-dimensional scanning platform driver, the ground wire of the signal wire of the photoacoustic signal receiving system and the ground wire of the photoacoustic signal preprocessing system are electrically connected through copper wires.
An imaging method based on the photoacoustic three-dimensional imaging device comprises the following steps:
(1) fixing a sample in a water tank, wherein the sample is positioned right below the micro-lens array, and filling deionized water in the water tank for coupling the photoacoustic signal;
(2) adjusting the vertical distance between the microlens array and the sample according to the magnitude of the A scanning signal, so that the sample is just positioned at the focus of the microlens array;
(3) the computer processing system simultaneously sends synchronous pulse trigger signals to the photoacoustic signal excitation system, the three-dimensional scanning system and the photoacoustic signal preprocessing system so as to perform parallel scanning, acquisition and storage;
(4) and the computer processing system reconstructs the three-dimensional photoacoustic images reflecting different laser absorption conditions of different structures of the sample through the acquired photoacoustic signals by a reconstruction algorithm.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the device has the greatest innovation point that the pulse laser is shaped into the linear light source by using the optical element, and then a plurality of light focuses are focused by using the micro-lens array to simultaneously excite the photoacoustic signal, so that the scanning route is simplified, and the high-speed photoacoustic three-dimensional imaging is realized.
(2) According to the invention, the special structure of the splayed linear acoustic detection array is utilized to ensure that no influence is generated between the linear excitation light path and the linear acoustic detection array, so that the laser source completely excites a sample and the acoustic detector can receive all the photoacoustic signals, and the signal-to-noise ratio of the photoacoustic image is ensured.
(3) The invention utilizes the optical fiber coupler to couple the laser to the single mode optical fiber, optical elements used in the laser shaping process are all provided with stainless steel packages, and the whole system has strong mobility and is convenient for practical application.
Drawings
Fig. 1 is a schematic system structure diagram of the apparatus of the present embodiment.
Fig. 2 is a schematic layout diagram of the linear array elements and 10 optical focuses of the splayed linear acoustic detection array according to this embodiment.
Fig. 3 is a schematic spatial diagram of the embodiment in which a microlens unit excites a sample and a splayed linear acoustic detection array receives photoacoustic signals.
FIG. 4 is an exemplary image of a three-dimensional volume being rapidly reconstructed using the apparatus and imaging method described in the embodiments.
Wherein: 1-a computer processing system; 2-nanosecond pulsed laser; 3-a coupled optical path; 4-linear laser generating lens; 5-a microlens array; 6-splayed linear acoustic detection array; 7-a signal amplification module and a band-pass filter; an 8-20 channel signal acquisition system; 9-three-dimensional scanning platform driver and scanning controller; 10-a three-dimensional scanning platform; 11-water tank.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
Example 1
As shown in fig. 1, the present embodiment discloses a simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging apparatus, which includes a computer processing system 1, a photoacoustic signal excitation system, a photoacoustic signal receiving system, a photoacoustic signal preprocessing system, and a three-dimensional scanning system, and the structure and function of each system are specifically described below.
Referring to fig. 1-3, the photoacoustic signal excitation system includes a nanosecond pulse laser 2, a coupling optical path 3, a linear laser generating lens 4 and a microlens array 5, wherein the coupling optical path 3 includes an optical fiber coupler, a single mode fiber, and an optical fiber collimator, the nanosecond pulse laser 2 preferably has a wavelength of 532nm, a maximum repetition frequency of 100KHz, and a pulse width of 7ns, the nanosecond pulse laser 2 couples laser to the single mode fiber through the optical fiber coupler, the coupling efficiency is greater than 80%, the pulse laser is transmitted to the optical fiber collimator through the single mode fiber, and the optical fiber collimator and the linear laser generating lens 4 are coaxially arranged and used for collimating the pulse laser emitted by the nanosecond pulse laser 2 to obtain parallel beams. The output end of the single-mode optical fiber is connected with the optical fiber collimator through an FC/PC standard joint.
In this embodiment, the linear laser generating lens 4 is configured to convert the parallel light beam into a linear laser with uniform energy distribution, and specifically, a powell prism is adopted, and the top of each lens has an aspheric curve for uniformly distributing optical power, so that the light intensity change at the center of the linear laser is less than 30%, and the linear light energy distribution is more uniform compared with a cylindrical lens.
In this embodiment, the microlens array 5 is fixed just below the linear laser generating lens 4, and the laser light can be entirely irradiated on the microlens array surface by adjusting the distance between the linear laser generating lens 4 and the microlens array 5. The micro-lens array 5 specifically adopts fused quartz lenses arranged in a square grid, only uses a single row of micro-lens units after optical customization cutting, the micro-lenses are circular or square, the interval is 300 micrometers, the filling factor is 100%, 10 light focuses are focused on the horizontal axis, and the focal length of each micro-lens is 18.6 millimeters. The micro lens array is plated with an antireflection film with the wavelength of 400-900 nm. The light transmittance is more than 99%, and linear laser can generate a focus of a light spot size limited by a near diffraction limit after being focused by the micro-lens array.
Referring to fig. 2 and 3, in the present embodiment, the photoacoustic signal receiving system employs a splayed linear acoustic detection array, the acoustic detection array is composed of two sub-ultrasonic transducer arrays 6 distributed in mirror symmetry, and the two sub-ultrasonic transducer arrays 6 are disposed on two sides of the microlens array 5 at a certain inclination angle. The two sub-ultrasonic transducer arrays are positioned at two sides of the light focus, the spatial distribution is shown in fig. 3, the array comprises 20 array elements, the spacing between the array elements is 0.2 mm, the central frequency is 15MHz, and the bandwidth is 60%. Linear light source arouses can not influence in the space with the collection of optoacoustic signal, guaranteed the complete arouse of light source and the complete receipt of acoustic signal, sub-ultrasonic transducer array is the plane array of constituteing by 10 bar array, the nearest bar array element of both sides distance of every light focus is responsible for gathering the optoacoustic signal that this light focus arouses, the signal that two array elements were gathered superposes to distinguish the signal distribution on the different degree of depth through the propagation velocity of optoacoustic signal in the medium and the time that the array element received the signal. Photoacoustic signals acquired by the splayed linear acoustic detection array in parallel are converted into electric signals, the electric signals are amplified through an amplifier, then the electric signals are filtered through a band-pass filter, and finally the electric signals are acquired and transmitted in parallel through a 20-channel signal acquisition system, and finally a computer processing system rapidly reconstructs photoacoustic three-dimensional images through a reconstruction algorithm.
In this embodiment, for fixing, the linear laser generating lens 4 and the micro lens array 5 are bonded to an optical stainless steel lens holder by epoxy glue. In addition, the number of the optical focuses and the number of the array elements of the linear sound detection array are not limited to the number of the optical focuses and the number of the array elements of the linear sound detection array, and the specification of the device can be flexibly customized according to requirements.
In this embodiment, the photoacoustic signal preprocessing system includes a three-stage multi-channel signal amplification module and a band-pass filter 7, which are connected in sequence, and the amplification gain is 65dB in total. In application, the signal processed by the band-pass filter is connected with a computer processing system through a 20-channel signal collector 8 to realize signal collection. The 20-channel signal collector, the 14-bit sampling precision and the 500MS/s sampling rate can collect and process the preprocessed signals in parallel.
In this embodiment, the three-dimensional scanning system includes a three-dimensional scanning platform 10, a three-dimensional scanning platform driver and a scanning controller 9, the scanning controller is connected to the computer processing system 1, and the computer processing system 1 controls the scanning speed and the scanning range of the three-dimensional scanning platform 10. The three-dimensional scanning platform comprises three one-dimensional ultrasonic motors, is provided with a grating ruler, adopts closed-loop control, and has a loading speed of more than 20mm/s, a positioning precision of 1 micron and a repeated positioning precision of 1 micron.
In this embodiment, the computer processing system 1 is connected to the photoacoustic signal excitation system, the photoacoustic signal preprocessing system, and the three-dimensional scanning system at the same time, and is configured to control the on/off of the photoacoustic signal excitation system, receive data processed by the photoacoustic signal preprocessing system, process the data, and perform reconstruction imaging to control the operation of the three-dimensional scanning system.
In this embodiment, the housing of the three-dimensional scanning platform, the ground line of the three-dimensional scanning platform driver, the ground line of the signal line of the photoacoustic signal receiving system, and the ground line of the photoacoustic signal preprocessing system are all electrically connected by copper wires.
An imaging method based on the photoacoustic three-dimensional imaging device comprises the following steps:
(1) fixing a sample in a water tank, wherein the sample is positioned right below the micro-lens array, and filling deionized water in the water tank for coupling the photoacoustic signal;
(2) adjusting the vertical distance between the microlens array and the sample according to the size of the scanning signal, so that the sample is just positioned at the focus of the microlens array;
(3) the computer processing system simultaneously carries out high-speed parallel scanning, acquisition and storage on the pulse laser, the three-dimensional scanning platform and the multi-channel signal acquisition system synchronous pulse trigger signals;
(4) and the computer processing system rapidly reconstructs the three-dimensional photoacoustic images reflecting different laser absorption conditions of different structures of the sample from the acquired photoacoustic signals through a reconstruction algorithm.
Fig. 4 is high-speed photoacoustic three-dimensional imaging performed by applying the apparatus and the imaging method of the above embodiment, and the imaging steps are as follows:
firstly, 5% of technical agar powder and 95% of water are mixed together to be made into a cuboid model in a container, and then two pencil leads with the length of about 7mm and the diameter of 0.5mm are taken out and obliquely inserted into the cuboid model. The model is integrally fixed to the bottom of the water tank, and the height of the water tank is adjusted to enable the positive center of the sample to be located at the focus of the micro-lens array, namely the maximum A-scanning signal. Computer control system simultaneously provides pulse laser, three-dimensional scanning platform and multi-channelThe synchronous pulse signals of the channel signal acquisition system are scanned and acquired at high speed, the finally acquired signals finish image reconstruction on MTLAB software by utilizing a three-dimensional reconstruction algorithm, and fig. 4 shows a photoacoustic image obtained after three-dimensional reconstruction of a sample, wherein the imaging range is 10 x 3mm3And the time is 2 minutes, so that high-speed photoacoustic three-dimensional imaging is realized.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging device is characterized by comprising a computer processing system, a photoacoustic signal excitation system, a photoacoustic signal receiving system, a photoacoustic signal preprocessing system and a three-dimensional scanning system, wherein:
the computer processing system controls the on-off of the photoacoustic signal excitation system, receives data processed by the photoacoustic signal preprocessing system, processes the data, reconstructs and images the data and controls the operation of the three-dimensional scanning system;
the photoacoustic signal excitation system firstly shapes pulse laser into a linear light source, then a plurality of light focuses are focused by utilizing a micro-lens array in the photoacoustic signal excitation system, multi-focus linear excitation is realized, and a plurality of light focuses simultaneously excite a sample to generate a multi-source photoacoustic signal;
the photoacoustic signal receiving system receives the multi-source photoacoustic signals in parallel;
the photoacoustic signal preprocessing system amplifies and filters a multi-source photoacoustic signal and sends the signal to a computer processing system through a multi-channel signal acquisition system;
the sample to be imaged is placed in a water tank, and the three-dimensional scanning system is used for adjusting the spatial positions of the water tank and the photoacoustic signal excitation system.
2. The photoacoustic three-dimensional imaging apparatus according to claim 1, wherein the photoacoustic signal excitation system comprises a nanosecond pulse laser, a coupling optical path, a linear laser generating lens and a micro-lens array, the coupling optical path couples pulse laser emitted by the nanosecond pulse laser into an optical fiber for transmission and collimation to obtain parallel light beams, the linear laser generating lens converts the parallel light beams into a linear laser with uniform energy distribution, the micro-lens array is fixed under the linear laser generating lens, and a single row of micro-lens unit array is adopted, so that the linear laser generates a row of optical focuses after being focused by the micro-lens array.
3. The photoacoustic three-dimensional imaging apparatus according to claim 2, wherein the coupling optical path comprises a fiber coupler, a single-mode fiber, and a fiber collimator, the nanosecond pulse laser couples laser light into the single-mode fiber through the fiber coupler, the pulse laser light is transmitted to the fiber collimator through the single-mode fiber, and the fiber collimator is coaxial with the linear laser generating lens.
4. The photoacoustic three-dimensional imaging apparatus of claim 3, wherein the single-mode fiber output end is connected to a fiber collimator through an FC/PC standard connector;
the linear laser generating lens adopts a Powell prism;
the micro-lens array is fused quartz lenses arranged in a square grid, only one row of micro-lens units are utilized after optical customization cutting, and the micro-lenses are round or square;
the micro lens array is plated with an anti-reflection film with the wavelength of 400-900 nm;
the linear laser generating lens and the micro lens array are bonded on an optical stainless steel lens base through epoxy resin glue.
5. The photoacoustic three-dimensional imaging apparatus according to claim 1, wherein the photoacoustic signal receiving system employs a splay linear acoustic detection array, the acoustic detection array is composed of two sub-ultrasonic transducer arrays distributed in mirror symmetry, each sub-ultrasonic transducer array is composed of a plurality of acoustic detection array elements, each optical focus and two acoustic detection array elements geometrically corresponding to two sides form an optical/acoustic excitation receiving matching unit, and the splay linear acoustic detection array collects excited multi-source photoacoustic signals by using a phased array technology.
6. The photoacoustic three-dimensional imaging apparatus of claim 5, wherein the two sub ultrasound transducer arrays are disposed at an oblique angle on two sides of the microlens array, and the microlens array focuses a row of light focus to pass through the middle portion of the two sub ultrasound transducer arrays and irradiate the surface of the sample.
7. The photoacoustic three-dimensional imaging apparatus of claim 1, wherein the photoacoustic signal preprocessing system comprises a three-stage multi-channel signal amplifying module, a band-pass filter and a multi-channel signal collecting system, which are connected in sequence.
8. The photoacoustic three-dimensional imaging apparatus of claim 1, wherein the three-dimensional scanning system comprises a three-dimensional scanning platform, a three-dimensional scanning platform driver, and a scan controller, the scan controller being connected to a computer processing system, the computer processing system controlling the scanning speed and the scanning range of the three-dimensional scanning platform.
9. The photoacoustic three-dimensional imaging apparatus of claim 8, wherein the three-dimensional scanning platform comprises three one-dimensional ultrasonic motors equipped with grating scales, and adopting closed-loop control;
the shell of the three-dimensional scanning platform, the ground wire of the three-dimensional scanning platform driver, the signal wire ground wire of the photoacoustic signal receiving system and the ground wire of the photoacoustic signal preprocessing system are electrically connected through copper wires.
10. An imaging method based on the photoacoustic three-dimensional imaging apparatus of any one of claims 1 to 9, comprising the steps of:
(1) fixing a sample in a water tank, wherein the sample is positioned right below the micro-lens array, and filling deionized water in the water tank for coupling the photoacoustic signal;
(2) adjusting the vertical distance between the microlens array and the sample according to the magnitude of the A scanning signal, so that the sample is just positioned at the focus of the microlens array;
(3) the computer processing system simultaneously sends synchronous pulse trigger signals to the photoacoustic signal excitation system, the three-dimensional scanning system and the photoacoustic signal preprocessing system so as to perform parallel scanning, acquisition and storage;
(4) and the computer processing system reconstructs the three-dimensional photoacoustic images reflecting different laser absorption conditions of different structures of the sample through the acquired photoacoustic signals by a reconstruction algorithm.
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