CN108717045A - A kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence - Google Patents
A kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence Download PDFInfo
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- CN108717045A CN108717045A CN201810569351.2A CN201810569351A CN108717045A CN 108717045 A CN108717045 A CN 108717045A CN 201810569351 A CN201810569351 A CN 201810569351A CN 108717045 A CN108717045 A CN 108717045A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1702—Systems in which incident light is modified in accordance with the properties of the material investigated with opto-acoustic detection, e.g. for gases or analysing solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
Abstract
The present invention provides a kind of ultrasound, three modality imaging systems of optoacoustic and fluorescence, including：Laser light source module, moving sweep module, data acquisition module and control process module；Laser light source module sends synchronous triggering signal for providing pulsed laser radiation to imaging sample and imaging sample being made to generate optoacoustic and fluorescence signal to data acquisition module and control process module；Moving sweep module, for carrying out 3-D scanning to sample according to predetermined movement parameter；Data acquisition module includes the optoacoustic/ultrasound/fluorescence signal generated for acquiring imaging sample；Control process module, start pulse signal for receiving pulsed laser light source, and moving sweep device is made to be scanned to target sample according to control instruction, while the target sample data that processing receives, it is merged optoacoustic/ultrasound/fluorescent image to obtain multi-modal reconstruction image.The imaging resolution and image taking speed that the present invention reduces in imaging sweep time, improves imaging system.
Description
Technical field
The present invention relates to technical field of photoacoustic, and in particular to a kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence.
Background technology
Photoacoustic imaging (Photoacoustic Imaging, PAI) is that the one kind being surging forward in recent years is based on biological tissue
Internal optical absorption characteristic difference has pure light using ultrasound as the novel lossless biomedical imaging method of information carrier
Study the high contrast features of picture and the deep penetration characteristic of pure ultrasonic imaging.Photoacoustic imaging is inhaled by the light detected in biological tissue
The photoacoustic signal that acceptor is generated by instantaneous thermoelastic effect replaces photon detection with sound wave, optical scattering is avoided from principle
Influence, breach optical imagery depth " soft limiting ", about 1mm, it can be achieved that depth reach 7cm deep tissues living imaging.Light
Acoustic imaging not only has the functional information for obtaining biological tissue, but also can carry out across scale high-resolution imaging to deep tissues
Photoacoustic imaging specifically includes three kinds of imaging modes at present：Opto-acoustic microscopic imaging technology (Photoacoustic
Microscopy, PAM), optoacoustic computed tomography imaging (Photoacousic Computed Tomography, PACT) and optoacoustic
Based endoscopic imaging (Photoacousic Endoscopy, PAE).Optoacoustic computed tomography, which is imaged PACT, has rapid data collection and reality
When imaging capability, the most clinical Transformation Potential in existing photoacoustic imaging pattern.But the imaging of optoacoustic computed tomography has scanning
The deficiencies of time is long, resolution ratio is relatively low and detection angle is limited.
Invention content
For the defects in the prior art, the present invention provides a kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence, realizes
Imaging resolution and image contrast are improved, and has the characteristics that image taking speed is fast.
To achieve the above object, the present invention provides following technical scheme：
The present invention provides a kind of ultrasound, three modality imaging systems of optoacoustic and fluorescence, including：Control process module, data
Acquisition module, moving sweep module and laser light source module；
It is provided with the data acquisition module in the moving sweep module and stores the sample stage of sample to be imaged, it is described
Moving sweep module drives the data acquisition module to be rotated horizontally and vertically moved, so that the institute in moving sweep module
It states data acquisition module and data acquisition is carried out to the sample to be imaged in sample stage；
Wherein, the control terminal of the moving sweep module is electrically connected with the control process module, so that at the control
Reason module controls the moving sweep module by the control terminal and is rotated horizontally and vertically moved；
The laser light source module is electrically connected with the control process module, and the laser light source module is to sample to be imaged
Emission pulse laser signal, and send laser trigger signal to the control process module；
The data acquisition module acquires sample reception ultrasonic wave and arteries and veins to be imaged to sample emission ultrasonic wave to be imaged
Ultrasonic signal, photoacoustic signal and the fluorescence signal generated after impulse optical signal；
The data acquisition module is electrically connected with the control process module, and the data acquisition module is by the ultrasound of acquisition
Signal, photoacoustic signal and fluorescence signal are sent to the control process module；
The control process module generates control instruction after receiving the laser trigger signal that the laser light source module is sent
It is instructed with controlling of sampling；And control instruction is sent so that the moving sweep module is according to preset to the moving sweep module
Kinematic parameter is moved；And controlling of sampling instruction is sent so that the data acquisition module is adopted to the data acquisition module
Collect ultrasonic signal, photoacoustic signal and fluorescence signal；
The control process module is believed receiving ultrasonic signal, photoacoustic signal and fluorescence that the data acquisition module is sent
It number is merged, obtains the multi-modal reconstruction image of sample to be imaged.
Further, the laser light source module, including：Pulse laser and multimode fibre coupling optical path；
The beam of laser coupling that the pulse laser generates is divided into N beams by the multimode fibre coupling optical path, so that N beams
Laser irradiation is on sample to be imaged.
Further, the multimode fibre coupling optical path, including：Multimode fibre, convex lens, diaphragm, fiber coupler and N
A lens group；
The laser that the pulse laser generates is collimated by the limitation of the diaphragm and by the convex lens
After focusing, coupled by the fiber coupler；Laser coupled is entered the multimode fibre by the fiber coupler
Entrance；The outlet of the multimode fibre is divided into N beams；
Wherein, the outlet per a branch of multimode fibre corresponds to a lens group.
Further, each described lens group includes：
In the planoconvex spotlight peace cylindrical mirror that the exit of multimode fibre is set gradually, the center of the outlet of multimode fibre,
The center of the center peace cylindrical mirror of planoconvex spotlight is on the same line.
Further, the number of the lens group is 8.
Further, the moving sweep module further includes：
Electric rotary table, the vertical displacement platform being arranged on electric rotary table, and the branch that is arranged under electric rotary table
Frame；
Wherein, the data acquisition module is provided on holder.
Further, the data acquisition module, including：Single array element ultrasonic transducer, CCD camera, filter amplifier and
Multichannel data acquisition device；
The acquisition output end of the list array element ultrasonic transducer is electrically connected with the input terminal of the filter amplifier, the filter
The output end of twt amplifier is electrically connected with the input terminal of the Multichannel data acquisition device；
The output end of the output end of the CCD camera and the Multichannel data acquisition device with the control process module
Input terminal electrical connection；
Wherein, single array element ultrasonic transducer is to sample emission ultrasonic wave to be imaged, and acquires sample to be imaged and generate
Ultrasonic signal and photoacoustic signal；
The CCD camera acquires the fluorescence signal that sample to be imaged generates, and the fluorescence signal of acquisition is sent to described
Control process module；
The Multichannel data acquisition device samples filtering and amplified ultrasonic signal and photoacoustic signal, and will adopt
The ultrasonic signal of sample and the photoacoustic signal of sampling are sent to the control process module.
As shown from the above technical solution, a kind of ultrasound of the present invention, three modality imaging system of optoacoustic and fluorescence, pass through
Moving sweep module is realized carries out circular scanning to the imaging section of sample to be imaged, reduces and is scanned in the imaging of optoacoustic computed tomography
Time；By laser light source module to sample emission laser beam to be imaged, so that sample surface to be imaged is formed ring-shaped light spot, be
Sample to be imaged provides laser irradiation evenly, improves the imaging resolution of imaging system；It is carried out by data acquisition module
Sparse sampling reduces data collection capacity and improves image taking speed；Optoacoustic, ultrasound and three modality of fluorescence can be in same width figures
As the information of the upper reflection composition of sample to be imaged and the different level of function.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is the present invention
Some embodiments for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is that the embodiment of the present invention provides a kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence general illustration；
Fig. 2 is that the embodiment of the present invention provides a kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence structural schematic diagram；
Fig. 3 is that the embodiment of the present invention provides a kind of ultrasonic, in three modality imaging system of optoacoustic and fluorescence all the way index path；
Fig. 4 is that the embodiment of the present invention provides that a kind of ultrasound, eight tunnel light paths bows in three modality imaging system of optoacoustic and fluorescence
View.
Specific implementation mode
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical solution in the embodiment of the present invention carries out clear, complete description, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art
The every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
An embodiment of the present invention provides a kind of ultrasound, three modality imaging systems of optoacoustic and fluorescence, referring to Fig. 1-Fig. 2, specifically
Including：
Control process module 100, data acquisition module 200, moving sweep module 300 and laser light source module 400；
It is provided with the data acquisition module 200 in the moving sweep module 300 and stores the sample of sample 500 to be imaged
This TV station, the moving sweep module 300 drive the data acquisition module 200 to be rotated horizontally and vertically moved, so that fortune
The data acquisition module 200 on dynamic scan module 300 carries out data acquisition to the sample to be imaged 500 in sample stage；
Wherein, the control terminal of the moving sweep module 300 is electrically connected with the control process module 100, so that described
Control process module 100 controls the moving sweep module 300 by the control terminal and is rotated horizontally and vertically moved, real
3-D scanning now is carried out to sample to be imaged；
The laser light source module 400 is electrically connected with the control process module 100, and the laser light source module 400 is right
500 emission pulse laser signal of sample to be imaged, and send laser trigger signal to the control process module 100；
In the specific implementation, the laser light source module, including：Pulse laser and multimode fibre coupling optical path；
The beam of laser coupling that the pulse laser generates is divided into N beams by the multimode fibre coupling optical path, so that N beams
Laser irradiation is on sample to be imaged.
In the laser light source module, pulse laser can be DYE lasers, and the laser pulse of output is Nd：YAF
(Neodymium-doped Yttrium Aluminium Garnet), and send laser in the form of laser triggering electric signal and touch
It signals to control process module.
The multimode fibre coupling optical path, including：Multimode fibre, convex lens, diaphragm, fiber coupler and N number of lens group；
The laser that the pulse laser generates is collimated by the limitation of the diaphragm and by the convex lens
After focusing, coupled by the fiber coupler；Laser coupled is entered the multimode fibre by the fiber coupler
Entrance；The outlet of the multimode fibre is divided into N beams；
The multimode fibre is used for the transmission of laser, and it is eight that the outlet one of multimode fibre, which divides, and laser goes out by multimode fibre
Mouth enters the lens group；
Wherein, the outlet per a branch of multimode fibre corresponds to a lens group, and the outlet of multimode fibre is eight, lens group
Number is eight.
The diaphragm is provided in the outlet of pulse laser, and the laser beam for reducing pulse laser generation is realized
Laser beam is limited.
Convex lens is arranged on the outside of diaphragm, will use two identical convex lenses；After convex lens 1 is placed on the outside of diaphragm, use
It is collimated in by the laser beam by diaphragm limitation of laser emitting, then passes through convex lens 2 and collimated is swashed
Light light beam is focused, and laser beam is coupled to the entrance of multimode fibre by fiber coupler.
Fiber coupler enters multimode fibre, the preferably fiber coupler of thorlabs for coupled laser light beam,
Being finely adjusted by the translation stage on coupler keeps coupling efficiency maximum.
Each described lens group includes：In the planoconvex spotlight peace cylinder that the exit of multimode fibre is set gradually
Mirror, the center of the outlet of multimode fibre, the center of the center peace cylindrical mirror of planoconvex spotlight are on the same line.
Laser beam first passes through after the outlet of multimode fibre injection and carries out list by flat cylindrical mirror after planoconvex spotlight collimates
Direction focusing forms strip light spots eventually by the anti-sound sheet glass of the higher light transmission of light transmittance in sample surface to be imaged.Multimode
Eight outlet ends of optical fiber correspond to eight groups of lens groups respectively, and final eight strip light spots form ring light in sample surface to be imaged
Spot.So that sample to be imaged generates photoacoustic signal and fluorescence signal.
Be the light all the way in the present embodiment referring to Fig. 3, system is designed as eight road light, and be with 4 ultrasonic transducers and
It is the light path part behind multimode fibre outlet in Fig. 3, Fig. 4 is the vertical view of the part light path for CCD camera.
The data acquisition module 200 emits ultrasonic wave to sample 500 to be imaged, and acquires sample 500 to be imaged and receive
Ultrasonic signal, photoacoustic signal and the fluorescence signal generated after ultrasonic wave and pulsed laser signal；
Wherein, sample to be imaged is when receiving pulsed laser signal and ultrasonic wave, generate optoacoustic effect, fluorescent effect and
Ultrasonic reflections, and provide photoacoustic signal, fluorescence signal and ultrasonic signal to the data acquisition module 200
The data acquisition module 200 is electrically connected with the control process module 100, and the data acquisition module 200 will
Ultrasonic signal, photoacoustic signal and the fluorescence signal of acquisition are sent to the control process module 100；
Wherein, the data acquisition module 200, including：Single array element ultrasonic transducer 210, CCD camera 220, filter and amplification
Device 230 and Multichannel data acquisition device 240；Single array element ultrasonic transducer 210 is even number.
The acquisition output end of the list array element ultrasonic transducer 210 is electrically connected with the input terminal of the filter amplifier 230,
The output end of the filter amplifier 230 is electrically connected with the input terminal of the Multichannel data acquisition device 240；
The output end of the output end of the CCD camera 220 and the Multichannel data acquisition device 240 at the control
Manage the input terminal electrical connection of module 100；
In data acquisition module 200, the list array element ultrasonic transducer 210 emits ultrasonic wave to sample 500 to be imaged,
And acquire ultrasonic signal and photoacoustic signal that sample 500 to be imaged generates；
The CCD camera 220 acquires the fluorescence signal that sample 500 to be imaged generates, and the fluorescence signal of acquisition is sent
To the control process module 100；
The Multichannel data acquisition device 240 can be Alazar digtizer series of products, the light for will receive
Acoustical signal and ultrasonic signal digitize and are transmitted to the control process module, to filtering and amplified ultrasonic signal and light
Acoustical signal is sampled, and the photoacoustic signal of the ultrasonic signal of sampling and sampling is sent to the control process module 100.
The control process module 100 generates control after receiving the laser trigger signal that the laser light source module 400 is sent
System instruction and controlling of sampling instruction；And control instruction is sent so that the moving sweep module to the moving sweep module 300
300 instructed according to controlling of sampling in preset kinematic parameter carry out 3-D scanning；And it is sent out to the data acquisition module 200
Controlling of sampling is sent to instruct so that the data acquisition module 200 acquires ultrasonic signal, photoacoustic signal and fluorescence signal；
After control process module 100 receives the laser trigger signal in the laser light source module 400, start control process
The kinematic parameter pre-seted and sampling parameter in module 100 generate control instruction and controlling of sampling instruction.
The control process module 100 to receive ultrasonic signal, photoacoustic signal that the data acquisition module 200 is sent and
Fluorescence signal is merged, and the multi-modal reconstruction image of sample 500 to be imaged is obtained.
The following provide control process module specific implementations：
Control process module can be divided into：Data acquisition control part, motion control portion and signal processing, firmly
Include personal computer and motion control card on part, motion control card is preferably ADT-8920A1, wherein；
Data acquisition control part is the human-computer interaction program based on LabVIEW, for data collecting card sampling ginseng
Number carries out Initialize installation, so that data collecting card can carry out data acquisition according to the sampling parameter set and preserve data；
The motion control portion is made of motion control card and human-computer interactive control program, for receiving pulsed laser light
The start pulse signal in source, synchronous averaging motion-control module generate motion control instruction according to the kinematic parameter pre-seted, and
Motion control instruction is sent to moving sweep device, is swept with driving it to carry out annular to target sample according to controlling of sampling instruction
It retouches；
The signal processing is executed by the personal computer, for believing the photoacoustic signal of target sample, ultrasound
Number and fluorescent signal data rebuild, and three's data are merged to obtain three-dimensional multimode state photoacoustic image.
In the specific implementation, the moving sweep module further includes：
Electric rotary table, the vertical displacement platform being arranged on electric rotary table, and the branch that is arranged under electric rotary table
Frame；
Wherein, the data acquisition module is provided on holder.
Moving sweep module 300 is used to, according to the kinematic parameter pre-seted, rotate single array element ultrasonic transducer 210 and CCD
Camera 220 carries out three dimensions scanning to sample to be imaged, further, electric rotary table, for receiving control processor hair
The control instruction sent, and photoacoustic signal is acquired according to control instruction driving single array element ultrasonic transducer and fluorescence signal is adopted
The rotation of the CCD camera of collection；Vertical electric displacement platform is treated into for vertically moving according to order-driven sample to be imaged
Decent multiple sections are scanned, to realize the 3-D scanning of sample.
It should be noted that the control that moving sweep mould electric rotary table in the block receives the transmission of control process module refers to
It is driven after order and the single array element ultrasonic transducer and CCD camera of optoacoustic, ultrasound and fluorescence signal acquisition is rotated, deflected to be imaged
First sampled point of sample；
Multi-channel data acquisition board in data acquisition module will carry out sample data acquisition to the first sampled point, and will adopt
The sample data collected is sent to control processor.
Single array element ultrasonic transducer in data acquisition module and CCD motors deflect to next sampled point in turn, the sampling
Point is corresponding according to controlling of sampling instruction；Data acquisition device is allow to acquire the sample data of next sampled point；
And so on, until acquisition completes controlling of sampling and instructs the corresponding sample data of all sampled points.
The signal processing is executed by the personal computer, for believing the photoacoustic signal of target sample, ultrasound
Number and fluorescent signal data rebuild, and three's data are merged to obtain three-dimensional multimode state photoacoustic image.
Photoacoustic signal, ultrasonic signal and the fluorescent signal data for the target sample that personal computer collects, according to
Spatial compound imaging mode merges the data that acquisition obtains, and carries out the reconstruction of photoacoustic image, wherein uses and is based on again
The annular photoacoustic image algorithm for reconstructing of compressed sensing builds the multi-modal reconstruction image of sample 500 to be imaged.
As can be seen from the above description, a kind of ultrasound provided in an embodiment of the present invention, three modality imaging system of optoacoustic and fluorescence, lead to
The circular scanning of multiple ultrasonic transducers and CCD camera realization to imaging section is crossed, multiple ultrasonic transducers are filled with filter and amplification
Reception and the processing system for setting composition multi-channel parallel, overcome sweep time in traditional Photoacoustic tomography long, image taking speed
Slow disadvantage；Ring-shaped light spot is formed in sample surface to be imaged using multi-path laser beam in light path, is provided more for sample to be imaged
Uniform laser irradiation improves the imaging resolution of imaging system；Data acquisition module uses sparse sampling method, reduces data
Collection capacity and improve image taking speed；Optoacoustic/three modality of ultrasound/fluorescence can reflect sample to be imaged on same piece image
The information of the different levels such as structure and function；The embodiment of the present invention realizes that optoacoustic is believed by way of being rebuild again after 3-D scanning
Number, three modalities of ultrasonic signal and fluorescence signal have that image taking speed is fast, improves imaging space compared with prior art
The advantages of resolution ratio and picture contrast.
It should be noted that herein, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also include other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.For the ordinary skill in the art, above-mentioned term can be understood in this hair as the case may be
Concrete meaning in bright.
In the specification of the present invention, numerous specific details are set forth.Although it is understood that the embodiment of the present invention can
To put into practice without these specific details.In some instances, well known method, structure and skill is not been shown in detail
Art, so as not to obscure the understanding of this description.Similarly, it should be understood that disclose in order to simplify the present invention and helps to understand respectively
One or more of a inventive aspect, in the above description of the exemplary embodiment of the present invention, each spy of the invention
Sign is grouped together into sometimes in single embodiment, figure or descriptions thereof.However, should not be by the method solution of the disclosure
It releases and is intended in reflection is following：The feature that i.e. the claimed invention requirement ratio is expressly recited in each claim is more
More features.More precisely, as the following claims reflect, inventive aspect is to be less than single reality disclosed above
Apply all features of example.Therefore, it then follows thus claims of specific implementation mode are expressly incorporated in the specific implementation mode,
Wherein each claim itself is as a separate embodiment of the present invention.It should be noted that in the absence of conflict, this
The feature in embodiment and embodiment in application can be combined with each other.The invention is not limited in any single aspect,
It is not limited to any single embodiment, is also not limited to the arbitrary combination and/or displacement of these aspects and/or embodiment.And
And can be used alone of the invention each aspect and/or embodiment or with other one or more aspects and/or its implement
Example is used in combination.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, it will be understood by those of ordinary skill in the art that：Its according to
So can with technical scheme described in the above embodiments is modified, either to which part or all technical features into
Row equivalent replacement；And these modifications or replacements, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme should all cover in the claim of the present invention and the range of specification.
Claims (7)
1. a kind of ultrasound, three modality imaging system of optoacoustic and fluorescence, which is characterized in that including：Control process module, data acquisition
Module, moving sweep module and laser light source module；
It is provided with the data acquisition module in the moving sweep module and stores the sample stage of sample to be imaged, the movement
Scan module drives the data acquisition module to be rotated horizontally and vertically moved, so that the number in moving sweep module
Data acquisition is carried out to the sample to be imaged in sample stage according to acquisition module；
Wherein, the control terminal of the moving sweep module is electrically connected with the control process module, so that the control process mould
Block controls the moving sweep module by the control terminal and is rotated horizontally and vertically moved；
The laser light source module is electrically connected with the control process module, and the laser light source module is to sample emission to be imaged
Pulsed laser signal, and send laser trigger signal to the control process module；
The data acquisition module acquires sample reception ultrasonic wave to be imaged and pulse and swashs to sample emission ultrasonic wave to be imaged
Ultrasonic signal, photoacoustic signal and the fluorescence signal generated after optical signal；
The data acquisition module is electrically connected with the control process module, and the data acquisition module believes the ultrasound of acquisition
Number, photoacoustic signal and fluorescence signal be sent to the control process module；
The control process module generates control instruction after receiving the laser trigger signal that the laser light source module is sent and adopts
Sample control instruction；And control instruction is sent so that the moving sweep module is according to preset movement to the moving sweep module
Parameter is moved；And it sends controlling of sampling to the data acquisition module and instructs so that the data collecting module collected is super
Acoustical signal, photoacoustic signal and fluorescence signal；
The control process module to receive ultrasonic signal, photoacoustic signal and fluorescence signal that the data acquisition module is sent into
Row fusion, obtains the multi-modal reconstruction image of sample to be imaged.
2. imaging system according to claim 1, which is characterized in that the laser light source module, including：Pulse laser
With multimode fibre coupling optical path；
The beam of laser coupling that the pulse laser generates is divided into N beams by the multimode fibre coupling optical path, so that N beam laser
It is radiated on sample to be imaged.
3. imaging system according to claim 2, which is characterized in that the multimode fibre coupling optical path, including：Multimode light
Fibre, convex lens, diaphragm, fiber coupler and N number of lens group；
The laser that the pulse laser generates is collimated and is gathered by the limitation of the diaphragm and by the convex lens
It is defocused, it is coupled by the fiber coupler；Laser coupled is entered entering for the multimode fibre by the fiber coupler
Mouthful；The outlet of the multimode fibre is divided into N beams；
Wherein, the outlet per a branch of multimode fibre corresponds to a lens group.
4. imaging system according to claim 3, which is characterized in that each described lens group includes：
In the planoconvex spotlight peace cylindrical mirror that the exit of multimode fibre is set gradually, the center of the outlet of multimode fibre, plano-convex
The center of the center peace cylindrical mirror of lens is on the same line.
5. imaging system according to claim 2, which is characterized in that the number of the lens group is 8.
6. imaging system according to claim 1, which is characterized in that the moving sweep module further includes：
Electric rotary table, the vertical displacement platform being arranged on electric rotary table, and the holder that is arranged under electric rotary table；
Wherein, the data acquisition module is provided on holder.
7. imaging system according to claim 1, which is characterized in that the data acquisition module, including：Single array element ultrasound
Energy converter, CCD camera, filter amplifier and Multichannel data acquisition device；
The acquisition output end of the list array element ultrasonic transducer is electrically connected with the input terminal of the filter amplifier, and the filtering is put
The output end of big device is electrically connected with the input terminal of the Multichannel data acquisition device；
The output end of the CCD camera and the output end of the Multichannel data acquisition device are defeated with the control process module
Enter end electrical connection；
Wherein, single array element ultrasonic transducer is to sample emission ultrasonic wave to be imaged, and acquires the super of sample generation to be imaged
Acoustical signal and photoacoustic signal；
The CCD camera acquires the fluorescence signal that sample to be imaged generates, and the fluorescence signal of acquisition is sent to the control
Processing module；
The Multichannel data acquisition device samples filtering and amplified ultrasonic signal and photoacoustic signal, and by sampling
Ultrasonic signal and the photoacoustic signal of sampling are sent to the control process module.
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| CN109567758A (en) * | 2018-12-29 | 2019-04-05 | 中国科学院深圳先进技术研究院 | Across the scale photoacoustic imaging system of one kind |
| CN110044481A (en) * | 2019-04-11 | 2019-07-23 | 南昌大学 | A kind of system and method compressing EO-1 hyperion micro-imaging |
| CN110530797A (en) * | 2019-09-25 | 2019-12-03 | 南京大学 | A kind of the double-mode imaging device and its application method of optoacoustic ghost imaging-fluorescence imaging |
| CN111528921A (en) * | 2020-05-27 | 2020-08-14 | 广东工业大学 | Information acquisition device |
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