CN106333650B - A kind of multiple dimensioned opto-acoustic microscopic imaging devices and methods therefor - Google Patents
A kind of multiple dimensioned opto-acoustic microscopic imaging devices and methods therefor Download PDFInfo
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- A61B5/0093—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
- A61B5/0095—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy by applying light and detecting acoustic waves, i.e. photoacoustic measurements
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Abstract
The present invention relates to a kind of multiple dimensioned opto-acoustic microscopic imaging device, fiber coupler is mounted on pulse laser, and one end of single mode optical fiber is connected on fiber coupler, and the other end of single mode optical fiber is connected on optical fiber collimator；The optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing lens are mounted on automatically controlled adjusting platform, and flat field scanning lens is mounted on the lower end of optics electric control focusing lens, and multifocal high frequency detector is mounted on the lower end of flat field scanning lens.The phenomenon that light focus point drift when the present invention avoids opto-acoustic microscopic imaging laser beam flying, it is able to achieve the multifocal optoacoustic confocal imaging on same position different depth, obtains high, uniform image resolution ratio and contrast in a certain range.The invention further relates to a kind of methods being imaged using multiple dimensioned opto-acoustic microscopic imaging device, belong to opto-acoustic microscopic imaging technical field.
Description
Technical field
The invention belongs to opto-acoustic microscopic imaging technical fields, and in particular to a kind of multiple dimensioned opto-acoustic microscopic imaging device and its
Method.
Background technique
Opto-acoustic microscopic imaging (Photoacoustic Microscopy, PAM) is the height to grow up on the basis of PAI
The lossless micro imaging method of resolution ratio is excited by the way that incident laser is carried out optical focus using the point light source after focusing
Tissue generates high frequency photoacoustic signal and is imaged.Using optical focus mode excitation, possess high contrast and high-resolution；Using
High frequency ultrasound receives, its imaging depth can achieve 10mm while guaranteeing resolution ratio.Common traditional living body high-resolution
When optical image technology --- multi-photon micro-imaging and optical coherent chromatographic imaging (OCT) are propagated in biological tissues due to light
Scattering properties, imaging depth is difficult " soft limiting " more than optical imagery.Fluorescent molecular tomography (FMT) and scattering light
Tomographic imaging (DOT) is although the molecules in living organisms imaging of 10mm depth, its not high (only millimeter of imaging space resolution ratio can be realized
Grade), opto-acoustic microscopic imaging can also realize the technology of micron dimension resolution ratio in millimeter-centimetre depth bounds, this is current
Not available for other all medical imagings and advantage that this technology is irreplaceable.This is but also PAM is diagnosed in clinical medicine
Aspect is with a wide range of applications, and the rapid development of photoacoustic imaging (PAI), indicates the one of Biologic Medical Image field in recent years
Secondary important technical innovation.
It is all to be imaged using the ultrasonic detector of single focus in current opto-acoustic microscopic imaging technology, due to list
The focal length of the ultrasonic detector of one focus, depth of focus and focused spot diameter are unique, and which results on the same position of biological tissue
It is difficult to obtain depth information abundant, it is difficult to reach and realize the imaging effect of Multi hiberarchy and multi scale in depth direction；At present simultaneously
Opto-acoustic microscopic imaging system in generally carry out optical focus using spherical focusing lens, but be scanned movement in laser beam
When, a spherical focusing lens can only be imaged on circular flat, i.e., optical focus can not keep in the same plane, have from
Burnt phenomenon；Although being usually used in current optoacoustic microtechnic there are also the opto-acoustic microscopic imaging device of zoom scan
Mechanical zoom, it is inconvenient, and also the precision of zoom is also inaccurate, it is difficult to and accurately cooperate multifocal high frequency detector real
Existing multifocal optoacoustic confocal imaging, thus affect the practical application effect of optoacoustic micro-imaging technology.
Summary of the invention
For the technical problems in the prior art, the object of the present invention is to provide a kind of multiple dimensioned optoacoustic it is micro- at
As device, it is able to achieve the multifocal optoacoustic confocal imaging on same position different depth, is obtained high, uniform in a certain range
Image resolution ratio and contrast, while improving detection sensitivity.
It is another object of the present invention to provide the imaging methods with above-mentioned multiple dimensioned opto-acoustic microscopic imaging device.It is real
The dynamic focusing imaging and multiple dimensioned imaging for having showed opto-acoustic microscopic imaging, ensure that the high-resolution and contrast of image, simultaneously
The more physiologic informations of depth direction can be extracted.
In order to achieve the above object, the present invention adopts the following technical scheme:
A kind of multiple dimensioned opto-acoustic microscopic imaging device, including computer system, automatically controlled adjusting platform, three-dimensional mobile station, arteries and veins
Laser, fiber coupler, single mode optical fiber, optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing lens, flat field is rushed to sweep
Retouch lens, multifocal high frequency detector, signal amplification module, automatically controlled adjusting platform controller, two-dimensional scanning mirrors controller；
The pulse laser, optics electric control focusing lens are connect with computer system signal；The two-dimensional scanning
The scanning galvanometer motor of galvanometer is connect with two-dimensional scanning mirrors controller signals, two-dimensional scanning mirrors controller and computer system
Signal connection；The automatically controlled adjusting platform is connect with automatically controlled adjusting platform controller signal, automatically controlled adjusting platform controller with
The connection of computer system signal；The multifocal high frequency detector is connect with signal amplification module signal, signal amplification module
It is connect with computer system signal；
The fiber coupler is mounted on pulse laser, and one end of single mode optical fiber is connected on fiber coupler,
The other end of single mode optical fiber is connected on optical fiber collimator；The optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing
Lens are mounted on automatically controlled adjusting platform, and flat field scanning lens is mounted on the lower end of optics electric control focusing lens, multifocal high frequency
Detector is mounted on the lower end of flat field scanning lens；The injection direction of the optical fiber collimator is towards the anti-of two-dimensional scanning mirrors
Penetrate mirror, the reflection direction of reflecting mirror is towards optics electric control focusing lens；The upper end of the three-dimensional mobile station is equipped with sample stage, institute
The multifocal high frequency detector stated is located at the top of sample stage.
Further, the automatically controlled adjusting platform is equipped with transverse and longitudinal mobile station, the end of transverse and longitudinal mobile station is equipped with branch
Arm fixed frame, the scanning galvanometer motor include the vertical motor of scanning galvanometer and scanning galvanometer cross motor, optical fiber collimator
It is mounted on the left side of support arm fixed frame, scanning galvanometer cross motor is mounted on the right side of support arm fixed frame, the vertical electricity of scanning galvanometer
Machine is mounted on the upside in the middle part of support arm fixed frame, and reflecting mirror is located at the right side of optical fiber collimator, the optics below reflecting mirror
Electric control focusing lens are mounted on support arm fixed frame.Provided with support arm fixed frame, each components easily can be reasonably installed,
Cooperate related components, completes photoacoustic imaging.
Further, the lower end of the optics electric control focusing lens is equipped with internal screw thread, the upper end of flat field scanning lens
It is equipped with external screw thread with lower end, the upper end of multifocal high frequency detector is equipped with internal screw thread.The center line of three on the same line,
Arranged coaxial is the key that guarantee to realize photoacoustic imaging purpose.
Further, the multifocal high frequency detector includes shell, focusing acoustic lenses, the annular being placed in shell
High frequency ultrasound detector；Annular high frequency ultrasound detector equipped with light hole is mounted on the inner wall of shell, is located at annular high frequency
Focusing acoustic lenses below ultrasonic detector are mounted on the lower part of outer casing inner wall；The annular high frequency ultrasound detector focuses
Acoustic lens, shell inner wall surround cavity, the shell is equipped with the water injection hole and drainage hole communicated with cavity；Described is poly-
Burnt acoustic lens is equipped with groove, which includes the spherical groove of cylinder shape groove and multiple different radiis successively to connect, more
The radius of a spherical groove gradually becomes smaller from top to bottom, and cylinder shape groove is located at the lower section of the spherical groove of least radius；It is described
Annular high frequency ultrasound detector connect with signal amplification module signal by signal wire, the shell is equipped with for signal
The outlet hole that line passes through.
Further, the annular high frequency ultrasound detector includes the glass fibre sound absorption being sequentially arranged from top to bottom
Plate, epoxy resin back lining materials, ring piezoelectric array element；The radius of the outer diameter of the ring piezoelectric array element and maximum spherical groove
Equal, the inner wall of the shell is equipped with annular convex platform, and the glass fibre acoustic board is placed on annular convex platform, the ring
Shape piezoelectricity array element is connect by signal wire with signal amplification module signal.
Further, imaging device further includes pedestal, fixed station；The three-dimensional mobile station is mounted on by pinboard
On pedestal, the automatically controlled adjusting stage+module positioned at three-dimensional mobile station rear adjusts consolidating for platform dextral on pedestal, positioned at automatically controlled
Determine platform to be mounted on the base, the automatically controlled adjusting platform controller and two-dimensional scanning mirrors controller are located under fixed station
Side；The three-dimensional mobile station is equipped with vertical adjusting knob, lateral adjustment knob and vertical adjustment knob.
Further, the computer system includes driving circuit, signal acquisition circuit, control system；Described
Driving circuit and signal acquisition circuit are connect with control system signal, and the signal amplification module and signal acquisition circuit are believed
Number connection, the pulse laser, optics electric control focusing lens, automatically controlled adjustings platform controller, two-dimensional scanning mirrors control
Device is connect with driving circuit signal.During photoacoustic imaging, can accurately it be controlled.
Further, the dominant frequency range of the annular high frequency ultrasound detector is 40MHz~70MHZ, bandwidth is
80%~110%, the range of sensitivity is -30dB~-50dB；The focusing acoustic lenses are by optics polystyrene material system
At, light transmittance >=92%, entrant sound rate >=95%.
A method of it is imaged using multiple dimensioned opto-acoustic microscopic imaging device, comprising the following steps:
(1) it according to Huygen's principle, is calculated using Helmhots-Kirchohoff integration theorem equipped with multiple and different radiuses
Spherical groove multifocal high frequency detector sound-filed simulation, it is burnt to find out sound in the spherical groove of different radii
Length, focused spot diameter, depth of focus, to calculate the area for sound,focal distribution situation of multifocal high frequency detector；
(2) water is injected from the water injection hole of multifocal high frequency detector with syringe pump the cavity of multifocal high frequency detector,
Make water full of entire cavity, until water is overflowed from the drainage hole of multifocal high frequency detector；
(3) sample is placed on sample stage, computer system control pulse laser issues pulse laser, pulse laser warp
Fiber coupler is coupled into single mode optical fiber, and pulse laser is conveyed into optical fiber collimator through single mode optical fiber again, and optical fiber collimator is by arteries and veins
Impulse light is incident on the reflecting mirror of two-dimensional scanning mirrors, and pulse laser is reflected into optics electric control focusing lens by reflecting mirror, arteries and veins
Impulse light enters flat field scanning lens after optics electric control focusing lens, and pulse laser is swept through optics electric control focusing lens and flat field
Enter multifocal high frequency detector after retouching lens focus, pulse laser reaches sample, sample after multifocal high frequency detector
The ultrasonic wave inspired is detected by multifocal high frequency detector, the Ultrasonic transformation that multifocal high frequency detector will detect
For electric signal and it is transferred to signal amplification module, photoacoustic signal is transmitted to computer system, department of computer science by signal amplification module
Photoacoustic signal is used for image reconstruction by system；
(4) computer system controls scanning galvanometer motor, scanning galvanometer motor driven by two-dimensional scanning mirrors controller
Reflecting mirror rotation, and then realize the spot scan of pulse laser；
(5) it is saturating to adjust optics electric control focusing by computer system for the calculated case being distributed according to the area for sound,focal of step (1)
The voltage or current of mirror, realizes the change of the optics electric control focusing focal length of lens, and is used cooperatively with multifocal high frequency detector,
The depth of the scan position of pulse laser optical focus is continuously adjusted, is repeated step (4)；
(6) with the photoacoustic signal image of the light focus spot scan of the multiple and different depth of computer system reconstructing.
Further, adjusting the depth of the scan position of pulse laser optical focus described in step (5) using following
Method carries out: changing the focal length of optics electric control focusing lens by computer system, each adjustable range of optical focus is 1 μm~50
μm, the maximum value that record photoacoustic signal occurs.
Generally speaking, the present invention has the advantage that
(1) present invention is able to achieve the multifocal optoacoustic confocal imaging on same position different depth, obtains a certain range
Interior high, uniform image resolution ratio and contrast, while improving detection sensitivity are more when optical focus and confocal area for sound,focal
The photoacoustic signal that focus high frequency detector obtains is maximum, detectivity highest.
(2) present invention is imaged using multifocal high frequency detector, and realizing has on multifocal high frequency detector acoustic axis
Multiple area for sound,focal, the diversity of multifocal high frequency detector focal length, depth of focus and focused spot diameter.On the same position of biological tissue
Depth information abundant can be obtained, realizes multi-level, more structures, multiple dimensioned imaging effect in depth direction convenient for reaching.
(3) present invention employs flat field scanning lenses, when pulsed laser beam is scanned movement, enable optical focus
It is enough to guarantee to scan in the same plane, avoid the optical focus defocus phenomenon of spherical focusing lens generation.
(4) present invention uses optics electric control focusing lens, makes optical focus only in the sound field coke waist of multifocal high frequency detector
On be scanned, optical focus is moved by micro-shifting, high in a certain range, uniform image resolution ratio and contrast is realized, mentions
High detectivity.
(5) present invention is cooperated using multifocal high frequency detector and optics electric control focusing lens, is realized with depth side
Upward multifocal optoacoustic confocal imaging, while photoacoustic imaging image resolution ratio is seriously non-uniform when having modified single layer focus scanning
Phenomenon ensure that the opto-acoustic microscopic imaging of the deep tissues under same resolution ratio.
(6) structure of multiple dimensioned opto-acoustic microscopic imaging device provided by the invention is simple, and manipulation is convenient, be conducive to promote and
Using.
(7) multifocal high frequency detector peace field scan lens technologies are applied to opto-acoustic microscopic imaging neck for the first time by the present invention
Domain, the phenomenon that having modified light focus point drift when opto-acoustic microscopic imaging laser beam flying.
(8) it the composite can be widely applied to Biologic Medical Image field.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the multiple dimensioned opto-acoustic microscopic imaging device of the present invention.
Fig. 2 is the diagrammatic cross-section of multifocal high frequency detector.
Fig. 3 is the Principles of Acoustics schematic diagram of multifocal high frequency detector.
Fig. 4 is the size by changing spherical groove radius R, ultrasound element diameter D, the change for the sound focal length f being calculated
Law line chart.
Fig. 5 is the size by changing spherical groove radius R, ultrasound element diameter D, the sound focused spot diameter d being calculated
Changing rule line chart.
Fig. 6 is the size by changing spherical groove radius R, ultrasound element diameter D, the change for the sound depth of focus L being calculated
Law line chart.
Wherein: 1 pedestal, 2 pinboards, 3 three-dimensional mobile stations, 4 sample stages, 5 multifocal high frequency detectors, the scanning of 6 flat fields are saturating
Mirror, 7 optics electric control focusing lens, 8 optical fiber collimators, 9 single mode optical fibers, 10 reflecting mirrors, 11 automatically controlled adjusting platforms, the amplification of 12 signals
Module, the 13 automatically controlled sound focusing cokes for adjusting platform controller, 14 fixed stations, 15 focus on light beam, the spherical groove that 16 radiuses are 6mm
The sound focusing focus for the spherical groove that the sound focusing focus for the spherical groove that point, 17 radiuses are 5mm, 18 radiuses are 4mm, 19 radiuses
For the sound focusing focus of the spherical groove of 3mm, 20 computer systems, 21 fiber couplers, 22 pulse lasers, 23 scanning galvanometers
Vertical motor, 24 scanning galvanometer cross motors, 25 two-dimensional scanning mirrors controllers.
Lateral adjustment knob in 3-1 three-dimensional mobile station, the vertical adjusting knob in 3-2 three-dimensional mobile station, 3-3 three-dimensional are moved
Vertical adjustment knob, 5-1 signal wire, 5-2 outlet hole, 5-3 cavity, 5-4 focusing acoustic lenses, 5-5 shell, 5-6 on dynamic platform is logical
Unthreaded hole, 5-7 glass fibre acoustic board, 5-8 epoxy resin back lining materials, 5-9 ring piezoelectric array element, 5-10 water injection hole, 5-11 row
Spherical groove that spherical groove that spherical groove that water hole, 5-12 radius are 6mm, 5-13 radius are 5mm, 5-14 radius are 4mm,
5-15 radius is that automatically controlled adjust of spherical groove, 5-16 cylinder shape groove, the side surface of 5-17 focusing acoustic lenses, the 11-1 of 3mm is put down
The automatically controlled transverse and longitudinal mobile station adjusted on platform of lifting shaft, 11-2 on platform.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
For sake of convenience, hereafter the described citing of front-rear direction up and down provides as follows: multifocal high frequency detector position
In the top of three-dimensional mobile station, the automatically controlled rear for adjusting platform and being located at three-dimensional mobile station, fixed station is located at automatically controlled adjusting platform
The right；Hereafter described up and down direction, that is, vertical direction, hereafter described left and right directions, that is, transverse direction, hereafter described front and back
Direction, that is, longitudinal direction.
As shown in connection with fig. 1, a kind of multiple dimensioned opto-acoustic microscopic imaging device, including computer system, automatically controlled adjusting platform, three
Tie up mobile station, pulse laser, fiber coupler, single mode optical fiber, optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing
Lens, flat field scanning lens, multifocal high frequency detector, signal amplification module, automatically controlled adjusting platform controller, two-dimensional scanning vibration
Mirror controller；Two-dimensional scanning mirrors are the micro- galvanometer of two-dimensional scanning, and pulse laser is nanosecoud pulse laser, two-dimensional scanning mirrors
Including reflecting mirror, scanning galvanometer motor.
The pulse laser, optics electric control focusing lens are connect with computer system signal；Computer system can
To control pulse laser, pulse laser is made to emit laser；Computer system, which can control, adjusts optics electric control focusing lens
Voltage or current, to change the focal length of optics electric control focusing lens.The scanning galvanometer motor and two-dimensional scanning shakes
The connection of mirror controller signals, two-dimensional scanning mirrors controller are connect with computer system signal；Computer system is swept by two dimension
It retouches galvanometer controller and sends instruction, scanning galvanometer motor and then driving reflecting mirror rotation to scanning galvanometer motor, to realize arteries and veins
The scanning of laser beam.The automatically controlled adjusting platform is connect with automatically controlled adjusting platform controller signal, automatically controlled adjusting platform control
Device processed is connect with computer system signal；Automatically controlled adjusting platform is equipped with lifting shaft and transverse and longitudinal mobile station, computer system pass through electricity
Control section platform controller sends to automatically controlled adjusting platform and instructs, so that the automatically controlled lifting shaft for adjusting platform achives up and down fortune
Dynamic, transverse and longitudinal mobile station realizes horizontal and vertical movement.The multifocal high frequency detector and signal amplification module signal connects
It connects, signal amplification module is connect with computer system signal；The photoacoustic signal detected is transferred to by multifocal high frequency detector
Photoacoustic signal is transferred to computer system again by signal amplification module, signal amplification module, computer system by photoacoustic signal into
Row photoacoustic imaging.
The fiber coupler is mounted on pulse laser, and one end of single mode optical fiber is connected on fiber coupler,
The other end of single mode optical fiber is connected on optical fiber collimator；The optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing
Lens are mounted on automatically controlled adjusting platform, and flat field scanning lens is mounted on the lower end of optics electric control focusing lens, multifocal high frequency
Detector is mounted on the lower end of flat field scanning lens.The injection direction of the optical fiber collimator is towards the anti-of two-dimensional scanning mirrors
Penetrate mirror, i.e., after pulse laser comes out from optical fiber collimator, pulse laser directive reflecting mirror.The reflection direction of reflecting mirror is towards optics
Electric control focusing lens, i.e. reflecting mirror can change the transmission direction of pulse laser, and pulse laser is reflected by a reflector rear incident light
It learns in electric control focusing lens, is rotated by scanning galvanometer motor driven reflecting mirror, can guarantee the reflection direction of reflecting mirror court always
To optics electric control focusing lens.The upper end of the three-dimensional mobile station is equipped with sample stage, the multifocal high frequency detector position
In the top of sample stage.
The upper end of the automatically controlled adjusting platform is equipped with transverse and longitudinal mobile station, and the end of transverse and longitudinal mobile station be (transverse and longitudinal mobile station
Front end) it is equipped with support arm fixed frame, the movement in horizontal and vertical direction may be implemented in transverse and longitudinal mobile station.The scanning galvanometer motor
Including the vertical motor of scanning galvanometer and scanning galvanometer cross motor, the vertical motor of scanning galvanometer and scanning galvanometer cross motor can be total to
With driving reflecting mirror rotation.Optical fiber collimator is mounted on the left side of support arm fixed frame, and scanning galvanometer cross motor is mounted on support arm
The right side of fixed frame, the vertical motor of scanning galvanometer are mounted on the upside in the middle part of support arm fixed frame, and reflecting mirror is located at optical fiber collimator
Right side, the optics electric control focusing lens below reflecting mirror are mounted on support arm fixed frame, and the lower part of support arm fixed frame is set
There is a horizontal plate, optics electric control focusing lens are mounted on the plate.Automatically controlled adjusting platform can realize support arm fixed frame
The movement of three-dimensional.
The lower end of the optics electric control focusing lens is equipped with internal screw thread, and the top and bottom of flat field scanning lens are equipped with
The upper end of external screw thread, multifocal high frequency detector is equipped with internal screw thread.The external screw thread and the automatically controlled tune of optics of flat field scanning lens upper end
The internal screw thread of focus lens lower end matches, the external screw thread of flat field scanning lens lower end and the interior spiral shell of multifocal high frequency detector upper end
Line matches.I.e. optics electric control focusing lens, flat field scanning lens, pass through screw thread between multifocal high frequency detector three and connect
It connects, securely and reliably, keeps the transmission of pulse laser unaffected.
As shown in Figure 1, Figure 2, the multifocal high frequency detector includes shell, focusing acoustic lenses, is placed in shell
Annular high frequency ultrasound detector.Annular high frequency ultrasound detector equipped with light hole is mounted on the inner wall of shell, focusing sound
Lens are located at the lower section of annular high frequency ultrasound detector, and the top of focusing acoustic lenses is mounted on the lower part of outer casing inner wall.Described
Annular high frequency ultrasound detector, focusing acoustic lenses, shell inner wall surround cavity, the shell is equipped with to be communicated with cavity
Water injection hole and drainage hole, drainage hole are located at the top of water injection hole.The focusing acoustic lenses are equipped with groove, which includes circle
The spherical groove of cylindrical groove and multiple different radiis successively to connect, i.e., spherical groove above and following spherical groove without
Seam transition connects, and the radius of multiple spherical grooves gradually becomes smaller from top to bottom, and the spherical surface that cylinder shape groove is located at least radius is recessed
The lower section of slot；From in shape, spherical groove is in the spherical shape that do not complete, and is a part of complete spherical；In the present invention, there are 4
The spherical groove of different radii.The annular high frequency ultrasound detector is connect by signal wire with signal amplification module signal,
The shell is equipped with the outlet hole passed through for signal wire.The shell is substantially cylindrical, outside focusing acoustic lenses
Contouring by it is cylindric and it is round table-like form, it is round table-like be located at columned lower end.
The annular high frequency ultrasound detector includes glass fibre acoustic board, the epoxy resin being sequentially arranged from top to bottom
Back lining materials, ring piezoelectric array element；Ring piezoelectric array element, glass fibre acoustic board, epoxy resin back lining materials, ring piezoelectric battle array
Member is in short cylindrical shape, is fixed together between three with optics epoxy resin glue；The diameter of glass fibre acoustic board is greater than
The diameter of epoxy resin back lining materials, the diameter of epoxy resin back lining materials are greater than the diameter of ring piezoelectric array element, epoxy resin
The inner wall of back lining materials and shell has certain space.The radius phase of the outer diameter of the ring piezoelectric array element and maximum spherical groove
Deng, all photoacoustic signals can in this way detected by ring piezoelectric array element, the inner wall of the shell is equipped with annular convex platform,
The glass fibre acoustic board is placed on annular convex platform, and the ring piezoelectric array element passes through signal wire and signal amplification module
Signal connection.
Imaging device further includes pedestal, fixed station；The three-dimensional mobile station is mounted on the base by pinboard, is located at
The automatically controlled adjusting stage+module at three-dimensional mobile station rear is mounted on bottom on pedestal, positioned at the automatically controlled fixed station for adjusting platform dextral
On seat, the automatically controlled adjusting platform controller and two-dimensional scanning mirrors controller are located at the lower section of fixed station；The three-dimensional
Mobile station is equipped with vertical adjusting knob, lateral adjustment knob and vertical adjustment knob.
The computer system includes driving circuit, signal acquisition circuit, control system；The driving circuit and letter
Number Acquisition Circuit is connect with control system signal, and the signal amplification module is connect with signal acquisition circuit signal, described
Pulse laser, optics electric control focusing lens, automatically controlled adjusting platform controller, two-dimensional scanning mirrors controller with driving electricity
The connection of road signal.Control system sends or receives signal by driving circuit, so that pulse laser, optics electric control focusing are saturating
Mirror, automatically controlled adjusting platform controller, two-dimensional scanning mirrors controller are in accurate working condition.
Driving circuit, signal acquisition circuit, control system, automatically controlled adjusting platform, three-dimensional mobile station, pulse laser, light
Fine coupler, single mode optical fiber, optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing lens, flat field scanning lens, signal
Amplification module, automatically controlled adjusting platform controller, two-dimensional scanning mirrors controller；Above components belong to existing maturation
Technology.Three-dimensional mobile station model: MBT602, production firm: Thorlabs (Soret wins Electro-optical Technology, INC. (US) 62 Martin Road, Concord, Massachusetts 017)；Two-dimensional scanning
Galvanometer model: M3-RS, production firm: New Scale；Flat field scanning lens model: SL-532-60-30Q, production firm:
Sino-Galvo (golden SeaBird development in science and technology Co., Ltd)；Optics electric control focusing lens model: EL-10-30, production firm:
Optotune.Pulse laser uses high energy laser bit slice, and pulse energy is preferably 1.76 μ j, and mean power is preferably
35mw, pulse width are preferably 8ns, and repetition rate maximum is preferably 30KHz.
Fiber coupler is equipped with three-dimensional adjustable structure, and coming with achromatic lens, (pulse that can transmit different wave length swashs
Light), connector uses FC/PC type interface modes, connect with single mode optical fiber.
Optical fiber collimator has achromatic lens (pulse laser that can transmit different wave length), single mode optical fiber and fiber optic collimator
Device is linked together by FC interface.
The circular tube shaped shell of multifocal high frequency detector is made of steel material, is unlikely to deform, is got rusty, and has noise screen
The effect of covering.
1 μm~the 5cm of focus adjustment range of optics electric control focusing lens.Optics electric control focusing lens can to pulse laser into
Line focusing.
The focal length of flat field scanning lens is 25mm, scanning angle is ± 20 °, and scanning range 10X10mm, equal spot are 2 μm.
Flat field scanning lens can also be focused pulse laser, play the role of further focusing, while flat field scanning lens can make
Optical focus is obtained to scan in the same plane.
Ring-shaped P VDF (Kynoar) ring piezoelectric array element of the annular high frequency ultrasound detector by 9 μm of thickness, thickness 2mm
Epoxy resin back lining materials, the annular glass fibre acoustic board of thickness 3mm and the conductive filamentary silver composition of positive and negative electrode, annular is high
The internal diameter of the light hole of frequency ultrasonic detector is 3mm, and the outer diameter of ring piezoelectric array element is 12m, dominant frequency range be 40MHz~
70MHZ, bandwidth are 80%~110%, the range of sensitivity is -30dB~-50dB；Annular high frequency ultrasound detector in the present invention
Dominant frequency be 50MHZ, bandwidth 90%, sensitivity be -35dB.
Focusing acoustic lenses are made by optics PS (polystyrene) material, have superior translucency and sound transparency, light transmittance
>=92%, entrant sound rate >=95%.The density of optics PS material is 1.05 × 103㎏/m3, the velocity of sound is 2.39 ㎞/s, and acoustic impedance is
2.52×106㎏/m3S, light transmittance 94%, entrant sound rate are 96%；Spherical groove surface on focusing acoustic lenses is smooth, circle
The bottom surface of cylindrical groove is flat and smooth, and translucency is good.Multi-functional focusing acoustic lenses least significant end disk is smooth, translucency
Well, the side surface of multi-functional focusing acoustic lenses is matte surface, it is difficult to light transmission entrant sound.It sees from top to bottom, 4 spherical grooves
Radius is respectively 6mm, 5mm, 4mm and 3mm, and the diameter of cylinder shape groove is 3mm, depth 1mm；Versatile acoustic lens are round table-like
Least significant end disk diameter be 4mm.
Signal amplification module includes signal amplifier and noise filter；Signal amplifier uses cascade amplifier, amplification
Multiple is 80dB, and bandwidth is 1MHz~200MHz；Noise filter is bandpass filter, pass band 10MHz~100MHz.
The sample rate of the signal acquisition circuit is 200MHz, resolution ratio 16bit.
A kind of multiple dimensioned opto-acoustic microscopic imaging method, comprising the following steps:
(1) it according to Huygen's principle, is calculated using Helmhots-Kirchohoff integration theorem equipped with multiple and different radiuses
Spherical groove multifocal high frequency detector sound-filed simulation, it is burnt to find out sound in the spherical groove of different radii
Length, focused spot diameter, depth of focus, to calculate the area for sound,focal distribution situation of multifocal high frequency detector；
(2) water is injected from the water injection hole of multifocal high frequency detector with syringe pump the cavity of multifocal high frequency detector,
Make water full of entire cavity, until water is overflowed from the drainage hole of multifocal high frequency detector；
(3) it combines shown in Fig. 1 and Fig. 3, sample is placed on sample stage, computer system control pulse laser issues arteries and veins
Impulse light, pulse laser are coupled into single mode optical fiber through fiber coupler, and pulse laser is conveyed into fiber optic collimator through single mode optical fiber again
Pulse laser is incident on the reflecting mirror of two-dimensional scanning mirrors by device, optical fiber collimator, and pulse laser is reflected into optics by reflecting mirror
In electric control focusing lens, pulse laser enters flat field scanning lens after optics electric control focusing lens, and pulse laser is through optics electricity
Enter multifocal high frequency detector after control focus lens peace field scan lens focus, pulse laser passes through multifocal high-frequency detection
Sample is reached after device, the ultrasonic wave that sample excitation goes out is detected by multifocal high frequency detector, and multifocal high frequency detector will
The Ultrasonic transformation detected is electric signal and is transferred to signal amplification module, and photoacoustic signal is transmitted to meter by signal amplification module
Photoacoustic signal is used for image reconstruction by calculation machine system, computer system；
(4) computer system controls scanning galvanometer motor, scanning galvanometer motor driven by two-dimensional scanning mirrors controller
Reflecting mirror rotation, and then realize the spot scan of pulse laser；
(5) it is saturating to adjust optics electric control focusing by computer system for the estimation situation being distributed according to the area for sound,focal of step (1)
The voltage or current of mirror, realizes the change of the optics electric control focusing focal length of lens, and is used cooperatively with multifocal high frequency detector,
The depth of the scan position of pulse laser optical focus is continuously adjusted, is repeated step (4)；Fig. 4 is by changing spherical groove half
The size of diameter R, ultrasound element diameter D, the changing rule line chart for the sound focal length f being calculated.Fig. 5 is recessed by changing spherical surface
The size of groove radius R, ultrasound element diameter D, the changing rule line chart for the sound focused spot diameter d being calculated.Fig. 6 is by changing
Become the size of spherical groove radius R, ultrasound element diameter D, the changing rule line chart for the sound depth of focus L being calculated.
(6) with the photoacoustic signal image of the light focus spot scan of the multiple and different depth of computer system reconstructing.Computer system
The focus scanning that LABVIEW program rebuild multiple and different depth can be used；
The depth that the scan position of pulse laser optical focus is adjusted described in step (5) is carried out using following methods: logical
The focal length that computer system changes optics electric control focusing lens is crossed, each adjustable range of optical focus is 1 μm~50 μm, records optoacoustic
The maximum value that signal occurs.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of multiple dimensioned opto-acoustic microscopic imaging device, it is characterised in that: including computer system, automatically controlled adjusting platform, three-dimensional
Mobile station, pulse laser, fiber coupler, single mode optical fiber, optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing are saturating
Mirror, flat field scanning lens, multifocal high frequency detector, signal amplification module, automatically controlled adjusting platform controller, two-dimensional scanning mirrors
Controller；
The pulse laser, optics electric control focusing lens are connect with computer system signal；The two-dimensional scanning mirrors
Scanning galvanometer motor connect with two-dimensional scanning mirrors controller signals, two-dimensional scanning mirrors controller and computer system signal
Connection；The automatically controlled adjusting platform is connect with automatically controlled adjusting platform controller signal, automatically controlled adjusting platform controller and calculating
The connection of machine system signal；The multifocal high frequency detector is connect with signal amplification module signal, signal amplification module and meter
The connection of calculation machine system signal；
The fiber coupler is mounted on pulse laser, and one end of single mode optical fiber is connected on fiber coupler, single mode
The other end of optical fiber is connected on optical fiber collimator；The optical fiber collimator, two-dimensional scanning mirrors, optics electric control focusing lens
It is mounted on automatically controlled adjusting platform, flat field scanning lens is mounted on the lower end of optics electric control focusing lens, multifocal high-frequency detection
Device is mounted on the lower end of flat field scanning lens；Injection reflection of the direction towards two-dimensional scanning mirrors of the optical fiber collimator
Mirror, the reflection direction of reflecting mirror is towards optics electric control focusing lens；The upper end of the three-dimensional mobile station is equipped with sample stage, described
Multifocal high frequency detector be located at the top of sample stage.
2. a kind of multiple dimensioned opto-acoustic microscopic imaging device described in accordance with the claim 1, it is characterised in that: the automatically controlled adjusting
Platform is equipped with transverse and longitudinal mobile station, and the end of transverse and longitudinal mobile station is equipped with support arm fixed frame, and the scanning galvanometer motor includes sweeping
The vertical motor of galvanometer and scanning galvanometer cross motor are retouched, optical fiber collimator is mounted on the left side of support arm fixed frame, and scanning galvanometer is horizontal
The right side of support arm fixed frame is mounted on to motor, the vertical motor of scanning galvanometer is mounted on the upside in the middle part of support arm fixed frame, reflection
Mirror is located at the right side of optical fiber collimator, and the optics electric control focusing lens below reflecting mirror are mounted on support arm fixed frame.
3. a kind of multiple dimensioned opto-acoustic microscopic imaging device described in accordance with the claim 1, it is characterised in that: the optics is automatically controlled
The lower end of focusing lens is equipped with internal screw thread, and the top and bottom of flat field scanning lens are equipped with external screw thread, multifocal high-frequency detection
The upper end of device is equipped with internal screw thread.
4. a kind of multiple dimensioned opto-acoustic microscopic imaging device described in accordance with the claim 1, it is characterised in that: the multifocal is high
Frequency detector includes shell, focusing acoustic lenses, the annular high frequency ultrasound detector being placed in shell；Annular equipped with light hole is high
Frequency ultrasonic detector is mounted on the inner wall of shell, and the focusing acoustic lenses below annular high frequency ultrasound detector are mounted on outer
The lower part of shell inner wall；The annular high frequency ultrasound detector, focusing acoustic lenses, shell inner wall surround cavity, described is outer
Shell is equipped with the water injection hole and drainage hole communicated with cavity；The focusing acoustic lenses are equipped with groove, which includes cylinder
The spherical groove of connected in star and multiple different radiis successively to connect, the radius of multiple spherical grooves gradually become smaller from top to bottom,
Cylinder shape groove is located at the lower section of the spherical groove of least radius；The annular high frequency ultrasound detector passes through signal wire and letter
The connection of number amplification module signal, the shell are equipped with the outlet hole passed through for signal wire.
5. a kind of multiple dimensioned opto-acoustic microscopic imaging device according to claim 4, it is characterised in that: the annular high frequency
Ultrasonic detector includes the glass fibre acoustic board, epoxy resin back lining materials, ring piezoelectric array element being sequentially arranged from top to bottom；
The outer diameter of the ring piezoelectric array element is equal with the maximum radius of spherical groove, and the inner wall of the shell is equipped with convex annular
Platform, the glass fibre acoustic board are placed on annular convex platform, and the ring piezoelectric array element is amplified by signal wire and signal
Module by signal connection.
6. a kind of multiple dimensioned opto-acoustic microscopic imaging device described in accordance with the claim 1, it is characterised in that: imaging device further includes
Pedestal, fixed station；The three-dimensional mobile station is mounted on the base by pinboard, the automatically controlled tune positioned at three-dimensional mobile station rear
Stage+module is saved on pedestal, is mounted on the base positioned at the automatically controlled fixed station for adjusting platform dextral, the automatically controlled adjusting is flat
Platform controller and two-dimensional scanning mirrors controller are located at the lower section of fixed station；The three-dimensional mobile station is revolved equipped with vertical adjusting
Button, lateral adjustment knob and vertical adjustment knob.
7. a kind of multiple dimensioned opto-acoustic microscopic imaging device described in accordance with the claim 1, it is characterised in that: the department of computer science
System includes driving circuit, signal acquisition circuit, control system；The driving circuit and signal acquisition circuit is and control system
Signal connection, the signal amplification module are connect with signal acquisition circuit signal, the automatically controlled tune of the pulse laser, optics
Focus lens, automatically controlled adjusting platform controller, two-dimensional scanning mirrors controller are connect with driving circuit signal.
8. a kind of multiple dimensioned opto-acoustic microscopic imaging device according to claim 4, it is characterised in that: the annular high frequency
The dominant frequency range of ultrasonic detector is 40MHz~70MHZ, bandwidth is 80%~110%, the range of sensitivity be -30dB~-
50dB；The focusing acoustic lenses are made of optics polystyrene material, light transmittance >=92%, entrant sound rate >=95%.
9. a kind of method being imaged using imaging device described in above-mentioned any one claim, it is characterised in that: packet
Include following steps:
(1) according to Huygen's principle, the ball equipped with multiple and different radiuses is calculated using Helmhots-Kirchohoff integration theorem
The sound-filed simulation of the multifocal high frequency detector of face groove finds out sound focal length in the spherical groove of different radii, coke
Spot diameter, depth of focus, to calculate the area for sound,focal distribution situation of multifocal high frequency detector；
(2) cavity for water being injected from the water injection hole of multifocal high frequency detector with syringe pump multifocal high frequency detector, makes water
Full of entire cavity, until water is overflowed from the drainage hole of multifocal high frequency detector；
(3) sample is placed on sample stage, computer system control pulse laser issues pulse laser, and pulse laser is through optical fiber
Coupler is coupled into single mode optical fiber, and pulse laser is conveyed into optical fiber collimator through single mode optical fiber again, and optical fiber collimator swashs pulse
Light is incident on the reflecting mirror of two-dimensional scanning mirrors, and pulse laser is reflected into optics electric control focusing lens by reflecting mirror, and pulse swashs
Light enters flat field scanning lens after optics electric control focusing lens, and pulse laser is saturating through optics electric control focusing lens peace field scan
Mirror enters multifocal high frequency detector after focusing, and pulse laser reaches sample, sample excitation after multifocal high frequency detector
Ultrasonic wave out is detected by multifocal high frequency detector, and the Ultrasonic transformation detected is electricity by multifocal high frequency detector
Signal is simultaneously transferred to signal amplification module, and photoacoustic signal is transmitted to computer system by signal amplification module, and computer system will
Photoacoustic signal is used for image reconstruction；
(4) computer system controls scanning galvanometer motor, the reflection of scanning galvanometer motor driven by two-dimensional scanning mirrors controller
Mirror rotation, and then realize the spot scan of pulse laser；
(5) calculated case being distributed according to the area for sound,focal of step (1) adjusts optics electric control focusing lens by computer system
Voltage or current, realizes the change of the optics electric control focusing focal length of lens, and is used cooperatively with multifocal high frequency detector, constantly
Ground adjusts the depth of the scan position of pulse laser optical focus, repeats step (4)；
(6) with the photoacoustic signal image of the light focus spot scan of the multiple and different depth of computer system reconstructing.
10. imaging method according to claim 9, it is characterised in that: adjustment pulse laser light focus described in step (5)
The depth of the scan position of point is carried out using following methods: change the focal length of optics electric control focusing lens by computer system,
The each adjustable range of optical focus is 1 μm~50 μm, the maximum value that record photoacoustic signal occurs.
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