CN109884869A - One kind calculating holographic imaging systems and method based on dicoria heterodyne - Google Patents
One kind calculating holographic imaging systems and method based on dicoria heterodyne Download PDFInfo
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- CN109884869A CN109884869A CN201910324392.XA CN201910324392A CN109884869A CN 109884869 A CN109884869 A CN 109884869A CN 201910324392 A CN201910324392 A CN 201910324392A CN 109884869 A CN109884869 A CN 109884869A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 22
- 241000085534 Dicoria Species 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 9
- 210000001747 pupil Anatomy 0.000 claims abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 8
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000009466 transformation Effects 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 230000000877 morphologic effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 15
- 238000010586 diagram Methods 0.000 description 3
- 239000011358 absorbing material Substances 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000001093 holography Methods 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
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- 238000001914 filtration Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The present invention relates to one kind to calculate holographic imaging systems and method based on dicoria heterodyne, belongs to photo-acoustic detection field.The system includes He-Ne laser source, beam expanding lens, spectroscope I, convex lens I, reflective mirror I, pupil I, AOM acousto-optic modulator, reflective mirror II, pupil II, convex lens II, spectroscope II, reflective mirror III, sample objective table, convex lens III, photodetector, multiplier, low-pass filter and computer unit.The present invention can recorde the phase information of cell by holographic digital imaging technology, the most comprehensive multidate information of cell be obtained, including the Morphologic Parameters that traditional phase contrast microscope can not obtain, such as cell thickness and volume.Cell can be distinguished in dicoria heterodyne scanning system imaging of the present invention to automatically correct in the threshold value of background, can be imaged by multiple exposure, be recalculated and restore imaging, further increase image definition.
Description
Technical field
The invention belongs to photo-acoustic detection fields, are related to a kind of based on dicoria heterodyne calculating holographic imaging systems and method.
Background technique
With the Digital Holography for improving and developing rapidly of computer and CCD technology, Digital Holography is not
It is recordable and the advantages of reproduce light field phase only to inherit holographic, and realizes the light field record to cell, storage and again
Existing total digitalization.The common microcytoscope observation of medicine needs to dye cell or marked, or passes through extraneous excitation
Light source analyzes cell imaging, but these labels and prolonged exposure often have certain injury to cell, even
The death for leading to cell using laser as coherent source, records object using principle of interference using digital holographic microscope (DHM)
The phase and amplitude information of wavefront by single holographic sample, the image of digital reconstruction sample different depth level, and reproduce mark
This true 3-D image.Vertical university T.C.Poon of Virginia, USA et al. is realized double using Mach-Zehnder interferometer
Pupil system.The impulse response (PSF) of incoherent optical processor is nonnegative real number, it generates one for the amplitude and phase of OTF
Fixed limitation, and the limitation of PSF can be overcome using double pupil image processing systems.
Summary of the invention
Holographic imaging systems and side are calculated based on dicoria heterodyne in view of this, the purpose of the present invention is to provide one kind
Method has stronger processing capacity to optical signal, and the complex and not high cell of transparency can be imaged out.
In order to achieve the above objectives, the invention provides the following technical scheme:
One kind calculating holographic imaging systems based on dicoria heterodyne, including He-Ne laser source, beam expanding lens, spectroscope I, convex
Lens I, reflective mirror I, pupil I, AOM acousto-optic modulator, reflective mirror II, pupil II, convex lens II, spectroscope II, reflective mirror
III, sample objective table, convex lens III, photodetector, multiplier, low-pass filter and computer unit;
Spectroscope I, spectroscope II, reflective mirror I, reflective mirror II and reflective mirror III and horizontal platform angle are 45 °, and He-Ne swashs
Light source keeps horizontal perpendicular to horizontal platform, beam expanding lens and horizontal platform;
AOM acousto-optic modulator is placed in the midpoint of spectroscope I and reflective mirror II, receives the light beam from spectroscope I, passes through
The different light beam of frequency is generated after AOM acousto-optic modulator, and passes to reflective mirror II;
Photodetector is in the focal range of the convex lens III of surface setting, is placed right above convex lens III
Sample objective table;Object under test is placed on sample objective table;
Pupil I is placed between reflective mirror I and spectroscope II, and pupil II is placed between reflective mirror II and convex lens II;
Multiplier includes sine multiplier and cosine multiplier;Signal from photodetector is distinguished after multiplier
Obtain optical heterodyne electric signal quadrature component and component in the same direction;Computer unit is entered by low-pass filter again.
Further, the He-Ne laser source wavelength is 632.8nm, power 1.5mW, chamber long 250 is mm, repetition rate
For 20Hz.
Further, the pupil I and pupil II pupil function p (x, y), the laser beam intensity c with He-Ne laser source
(x, y) meets Fourier transformation.
Further, the sample objective table is transparent glass container, and glass container inner wall, which is coated with one layer, to be used to weaken
The sound-absorbing material of the reflection interference of photoacoustic waves, glass container surface are covered with the coating for preventing attaching.
Holographic imaging method is calculated based on dicoria heterodyne based on the system, the described method comprises the following steps:
S1: object under test is placed on sample objective table, and the cell of object under test is made to be maintained at the focal length of convex lens III
In range;
S2: starting He-Ne laser source irradiates the beam splitting that light is realized into spectroscope I with horizontal direction;
S3: the intensity for the light that photodetector is come in by detection transmission measures, and then carries out signal reconstruction, then pass
It is defeated to arrive computer unit.
The beneficial effects of the present invention are:
(1) comparison can only provide magnitude images with conventional optical microscope, and active somatic cell belongs to phase type, and intensity variation is not
Obviously, light cell and around spread speed it is different, it may appear that phase delay is difficult clear discernable cell edge.By complete
Breath digital imaging technology can recorde the phase information of cell, obtain the most comprehensive multidate information of cell, including tradition difference is shown
The Morphologic Parameters that micro mirror can not obtain, such as cell thickness and volume.
(2) it can distinguish cell in the imaging of this dicoria heterodyne scanning system and automatically corrected in the threshold value of background, it can be through
Excessive times exposure image recalculates and restores imaging, further increases image definition.
(3) image capture is either individual cells or certain region group, are also possible to multiple regions group, in addition may be used also
With the imaging that is delayed.Gained picture can be both presented with conventional 2D, be also possible to the 3D rendering rebuild.
(4) dicoria heterodyne digital hologram is taken to scan, traditional optical scanner is a kind of noncoherent detection, for photoelectricity
Detector intelligently exports the amplitude information of record and phase information is all lost.And the device is scanned using dicoria heterodyne, is made
Original optical frequency 0 is modulated by acousto-optic frequency shifters with two beam laser and becomes ω 0+ Ω, to make 0 object light of frequency ω and frequency
Reference light for ω 0+ Ω is interfered, and this method can both save amplitude information or save phase information.
(5) strict control is carried out to system simultaneously by two pupils, making space filtering no longer becomes single, and system is to light
The processing of information is greatly enhanced.
(6) it is scanned using dicoria heterodyne digital hologram, using optical heterodyne detection and the advantages of multiplexer, can be kept away
Exempt from Zero-order diffractive item and grips the interference of picture altogether.
Other advantages, target and feature of the invention will be illustrated in the following description to a certain extent, and
And to a certain extent, based on will be apparent to those skilled in the art to investigating hereafter, Huo Zheke
To be instructed from the practice of the present invention.Target of the invention and other advantages can be realized by following specification and
It obtains.
Detailed description of the invention
To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is made below in conjunction with attached drawing excellent
The detailed description of choosing, in which:
Fig. 1 is schematic structural view of the invention.
Appended drawing reference: 1-He-Ne laser source, 2- beam expanding lens, 3- spectroscope I, 4- convex lens I, 5- reflective mirror I, 6- pupil
I, 7-AOM acousto-optic modulator, 8- reflective mirror II, 9- pupil II, 10- convex lens II, 11- spectroscope II, 12- reflective mirror III,
13- sample objective table, 14- convex lens III, 15- photodetector, 16- multiplier, 17- low-pass filter, 18- computer list
Member.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from
Various modifications or alterations are carried out under spirit of the invention.It should be noted that diagram provided in following embodiment is only to show
Meaning mode illustrates basic conception of the invention, and in the absence of conflict, the feature in following embodiment and embodiment can phase
Mutually combination.
Wherein, the drawings are for illustrative purposes only and are merely schematic diagrams, rather than pictorial diagram, should not be understood as to this
The limitation of invention;Embodiment in order to better illustrate the present invention, the certain components of attached drawing have omission, zoom in or out, not
Represent the size of actual product;It will be understood by those skilled in the art that certain known features and its explanation may be omitted and be in attached drawing
It is understood that.
The same or similar label correspond to the same or similar components in the attached drawing of the embodiment of the present invention;It is retouched in of the invention
In stating, it is to be understood that if there is the orientation or positional relationship of the instructions such as term " on ", "lower", "left", "right", "front", "rear"
To be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description of the present invention and simplification of the description, rather than indicate or
It implies that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore is described in attached drawing
The term of positional relationship only for illustration, is not considered as limiting the invention, for the ordinary skill of this field
For personnel, the concrete meaning of above-mentioned term can be understood as the case may be.
The invention discloses a kind of holographic imaging systems of Based on Dual-Aperture diaphragm heterodyne scanning, as shown in Figure 1, including He-Ne
Laser source 1, beam expanding lens 2, spectroscope I 3, convex lens I 4, reflective mirror I 5, pupil I 6, AOM acousto-optic modulator 7, reflective mirror II
8, pupil II 9, convex lens II 10, spectroscope II 11, reflective mirror III 12, sample objective table 13, convex lens III 14, light
Electric explorer 15, multiplier 16, low-pass filter 17 and computer unit 18.
Wherein, spectroscope and reflective mirror and horizontal platform are in 45° angle, and He-Ne laser source 1 is perpendicular to horizontal platform, 2 water of beam expanding lens
It puts down in horizontal platform;
AOM acousto-optic modulator 7 is placed in the middle of spectroscope I 3 and reflective mirror II 8, receives from spectroscope I 3
Light beam, after AOM acousto-optic modulator 7 generate frequency difference light beam pass to reflective mirror II 8;
Photodetector 15 be in surface setting convex lens III 14 focal range in, convex lens III 14 just on
Place sample objective table 13 in side;Object under test is placed on sample objective table 13;
Pupil I 6 is placed among reflective mirror I 5 and spectroscope II 11, and pupil II 9 is placed in reflective mirror II 8 and convex lens
Between II 10;
Multiplier 16 includes sine multiplier and cosine multiplier;Signal from photodetector 15 passes through multiplier 16
Respectively obtain afterwards optical heterodyne electric signal quadrature component and component in the same direction;Computer unit is entered by low-pass filter 17 again
18。
The invention discloses the detection methods of the holographic imaging systems of above-mentioned Based on Dual-Aperture diaphragm heterodyne scanning, including following step
It is rapid:
S1: red blood cell sample is placed on sample objective table, makes cell to be measured in focal range.
S2: starting He-Ne laser source irradiates the beam splitting that light is realized into spectroscope with horizontal direction.
S3: photodetector carries out photoacoustic signal reconstruction by the strength information that detection transmits light, and exposure is arranged
Time.
In step sl, the sample objective table is transparent glass container, and glass container inner wall is coated with one layer of use
Come weaken photoacoustic waves reflection interference sound-absorbing material, vessel surface coating prevents it from sticking.By red blood cell culture solution according to right
Ratio is answered to adjust.
In step s 2, He-Ne laser source wavelength is 632.8nm, power 1.5mW, repetition rate 20Hz.
In step s3, photodetector will measure the light intensity of input, carries out signal reconstruction and passes to stored digital
Oscillography unit.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of the technical program, should all be covered in the present invention
Scope of the claims in.
Claims (5)
1. one kind calculates holographic imaging systems based on dicoria heterodyne, it is characterised in that: including He-Ne laser source, beam expanding lens, divide
Light microscopic I, convex lens I, reflective mirror I, pupil I, AOM acousto-optic modulator, reflective mirror II, pupil II, convex lens II, spectroscope II,
Reflective mirror III, sample objective table, convex lens III, photodetector, multiplier, low-pass filter and computer unit;
Spectroscope I, spectroscope II, reflective mirror I, reflective mirror II and reflective mirror III and horizontal platform angle are 45 °, He-Ne laser source
Perpendicular to horizontal platform, beam expanding lens and horizontal platform keep horizontal;
AOM acousto-optic modulator is placed in the midpoint of spectroscope I and reflective mirror II, the light beam from spectroscope I is received, by AOM
The different light beam of frequency is generated after acousto-optic modulator, and passes to reflective mirror II;
Photodetector is in the focal range of the convex lens III of surface setting, places sample right above convex lens III
Objective table;Object under test is placed on sample objective table;
Pupil I is placed between reflective mirror I and spectroscope II, and pupil II is placed between reflective mirror II and convex lens II;
Multiplier includes sine multiplier and cosine multiplier;Signal from photodetector respectively obtains after multiplier
The quadrature component of optical heterodyne electric signal and component in the same direction;Computer unit is entered by low-pass filter again.
2. according to claim 1 a kind of based on dicoria heterodyne calculating holographic imaging systems, it is characterised in that: described
He-Ne laser source wavelength is 632.8nm, power 1.5mW, chamber long 250 is mm, repetition rate 20Hz.
3. according to claim 1 a kind of based on dicoria heterodyne calculating holographic imaging systems, it is characterised in that: the light
The pupil function p (x, y) of pupil I and pupil II meets Fourier transformation with the laser beam intensity c (x, y) of He-Ne laser source.
4. according to claim 1 a kind of based on dicoria heterodyne calculating holographic imaging systems, it is characterised in that: the sample
This objective table is transparent glass container, and glass container inner wall is coated with one layer of sound absorption for being used to weaken the reflection interference of photoacoustic waves
Material, glass container surface are covered with the coating for preventing attaching.
5. calculating holographic imaging method, feature based on dicoria heterodyne based on system described in any one of Claims 1 to 4
It is: the described method comprises the following steps:
S1: object under test is placed on sample objective table, and the cell of object under test is made to be maintained at the focal range of convex lens III
It is interior;
S2: starting He-Ne laser source irradiates the beam splitting that light is realized into spectroscope I with horizontal direction;
S3: the intensity for the light that photodetector is come in by detection transmission measures, and then carries out signal reconstruction, then be transferred to
Computer unit.
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Cited By (2)
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CN112947023A (en) * | 2021-02-03 | 2021-06-11 | 昆明理工大学 | Optical scanning holographic three-dimensional object real-time identification system and method |
CN113325683A (en) * | 2021-05-11 | 2021-08-31 | 昆明理工大学 | Off-axis scanning holographic three-dimensional target real-time joint transformation recognition system and method |
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