CN109884869A - One kind calculating holographic imaging systems and method based on dicoria heterodyne - Google Patents

One kind calculating holographic imaging systems and method based on dicoria heterodyne Download PDF

Info

Publication number
CN109884869A
CN109884869A CN201910324392.XA CN201910324392A CN109884869A CN 109884869 A CN109884869 A CN 109884869A CN 201910324392 A CN201910324392 A CN 201910324392A CN 109884869 A CN109884869 A CN 109884869A
Authority
CN
China
Prior art keywords
reflective mirror
spectroscope
convex lens
pupil
heterodyne
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910324392.XA
Other languages
Chinese (zh)
Inventor
王岫鑫
蒋帅帅
蒋宇皓
杨慧君
田银
赵德春
王伟
李章勇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing University of Post and Telecommunications
Original Assignee
Chongqing University of Post and Telecommunications
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing University of Post and Telecommunications filed Critical Chongqing University of Post and Telecommunications
Priority to CN201910324392.XA priority Critical patent/CN109884869A/en
Publication of CN109884869A publication Critical patent/CN109884869A/en
Pending legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Holo Graphy (AREA)

Abstract

The present invention relates to one kind to calculate holographic imaging systems and method based on dicoria heterodyne, belongs to photo-acoustic detection field.The system includes He-Ne laser source, beam expanding lens, spectroscope I, convex lens I, reflective mirror I, pupil I, AOM acousto-optic modulator, reflective mirror II, pupil II, convex lens II, spectroscope II, reflective mirror III, sample objective table, convex lens III, photodetector, multiplier, low-pass filter and computer unit.The present invention can recorde the phase information of cell by holographic digital imaging technology, the most comprehensive multidate information of cell be obtained, including the Morphologic Parameters that traditional phase contrast microscope can not obtain, such as cell thickness and volume.Cell can be distinguished in dicoria heterodyne scanning system imaging of the present invention to automatically correct in the threshold value of background, can be imaged by multiple exposure, be recalculated and restore imaging, further increase image definition.

Description

One kind calculating holographic imaging systems and method based on dicoria heterodyne
Technical field
The invention belongs to photo-acoustic detection fields, are related to a kind of based on dicoria heterodyne calculating holographic imaging systems and method.
Background technique
With the Digital Holography for improving and developing rapidly of computer and CCD technology, Digital Holography is not It is recordable and the advantages of reproduce light field phase only to inherit holographic, and realizes the light field record to cell, storage and again Existing total digitalization.The common microcytoscope observation of medicine needs to dye cell or marked, or passes through extraneous excitation Light source analyzes cell imaging, but these labels and prolonged exposure often have certain injury to cell, even The death for leading to cell using laser as coherent source, records object using principle of interference using digital holographic microscope (DHM) The phase and amplitude information of wavefront by single holographic sample, the image of digital reconstruction sample different depth level, and reproduce mark This true 3-D image.Vertical university T.C.Poon of Virginia, USA et al. is realized double using Mach-Zehnder interferometer Pupil system.The impulse response (PSF) of incoherent optical processor is nonnegative real number, it generates one for the amplitude and phase of OTF Fixed limitation, and the limitation of PSF can be overcome using double pupil image processing systems.
Summary of the invention
Holographic imaging systems and side are calculated based on dicoria heterodyne in view of this, the purpose of the present invention is to provide one kind Method has stronger processing capacity to optical signal, and the complex and not high cell of transparency can be imaged out.
In order to achieve the above objectives, the invention provides the following technical scheme:
One kind calculating holographic imaging systems based on dicoria heterodyne, including He-Ne laser source, beam expanding lens, spectroscope I, convex Lens I, reflective mirror I, pupil I, AOM acousto-optic modulator, reflective mirror II, pupil II, convex lens II, spectroscope II, reflective mirror III, sample objective table, convex lens III, photodetector, multiplier, low-pass filter and computer unit;
Spectroscope I, spectroscope II, reflective mirror I, reflective mirror II and reflective mirror III and horizontal platform angle are 45 °, and He-Ne swashs Light source keeps horizontal perpendicular to horizontal platform, beam expanding lens and horizontal platform;
AOM acousto-optic modulator is placed in the midpoint of spectroscope I and reflective mirror II, receives the light beam from spectroscope I, passes through The different light beam of frequency is generated after AOM acousto-optic modulator, and passes to reflective mirror II;
Photodetector is in the focal range of the convex lens III of surface setting, is placed right above convex lens III Sample objective table;Object under test is placed on sample objective table;
Pupil I is placed between reflective mirror I and spectroscope II, and pupil II is placed between reflective mirror II and convex lens II;
Multiplier includes sine multiplier and cosine multiplier;Signal from photodetector is distinguished after multiplier Obtain optical heterodyne electric signal quadrature component and component in the same direction;Computer unit is entered by low-pass filter again.
Further, the He-Ne laser source wavelength is 632.8nm, power 1.5mW, chamber long 250 is mm, repetition rate For 20Hz.
Further, the pupil I and pupil II pupil function p (x, y), the laser beam intensity c with He-Ne laser source (x, y) meets Fourier transformation.
Further, the sample objective table is transparent glass container, and glass container inner wall, which is coated with one layer, to be used to weaken The sound-absorbing material of the reflection interference of photoacoustic waves, glass container surface are covered with the coating for preventing attaching.
Holographic imaging method is calculated based on dicoria heterodyne based on the system, the described method comprises the following steps:
S1: object under test is placed on sample objective table, and the cell of object under test is made to be maintained at the focal length of convex lens III In range;
S2: starting He-Ne laser source irradiates the beam splitting that light is realized into spectroscope I with horizontal direction;
S3: the intensity for the light that photodetector is come in by detection transmission measures, and then carries out signal reconstruction, then pass It is defeated to arrive computer unit.
The beneficial effects of the present invention are:
(1) comparison can only provide magnitude images with conventional optical microscope, and active somatic cell belongs to phase type, and intensity variation is not Obviously, light cell and around spread speed it is different, it may appear that phase delay is difficult clear discernable cell edge.By complete Breath digital imaging technology can recorde the phase information of cell, obtain the most comprehensive multidate information of cell, including tradition difference is shown The Morphologic Parameters that micro mirror can not obtain, such as cell thickness and volume.
(2) it can distinguish cell in the imaging of this dicoria heterodyne scanning system and automatically corrected in the threshold value of background, it can be through Excessive times exposure image recalculates and restores imaging, further increases image definition.
(3) image capture is either individual cells or certain region group, are also possible to multiple regions group, in addition may be used also With the imaging that is delayed.Gained picture can be both presented with conventional 2D, be also possible to the 3D rendering rebuild.
(4) dicoria heterodyne digital hologram is taken to scan, traditional optical scanner is a kind of noncoherent detection, for photoelectricity Detector intelligently exports the amplitude information of record and phase information is all lost.And the device is scanned using dicoria heterodyne, is made Original optical frequency 0 is modulated by acousto-optic frequency shifters with two beam laser and becomes ω 0+ Ω, to make 0 object light of frequency ω and frequency Reference light for ω 0+ Ω is interfered, and this method can both save amplitude information or save phase information.
(5) strict control is carried out to system simultaneously by two pupils, making space filtering no longer becomes single, and system is to light The processing of information is greatly enhanced.
(6) it is scanned using dicoria heterodyne digital hologram, using optical heterodyne detection and the advantages of multiplexer, can be kept away Exempt from Zero-order diffractive item and grips the interference of picture altogether.
Other advantages, target and feature of the invention will be illustrated in the following description to a certain extent, and And to a certain extent, based on will be apparent to those skilled in the art to investigating hereafter, Huo Zheke To be instructed from the practice of the present invention.Target of the invention and other advantages can be realized by following specification and It obtains.
Detailed description of the invention
To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is made below in conjunction with attached drawing excellent The detailed description of choosing, in which:
Fig. 1 is schematic structural view of the invention.
Appended drawing reference: 1-He-Ne laser source, 2- beam expanding lens, 3- spectroscope I, 4- convex lens I, 5- reflective mirror I, 6- pupil I, 7-AOM acousto-optic modulator, 8- reflective mirror II, 9- pupil II, 10- convex lens II, 11- spectroscope II, 12- reflective mirror III, 13- sample objective table, 14- convex lens III, 15- photodetector, 16- multiplier, 17- low-pass filter, 18- computer list Member.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from Various modifications or alterations are carried out under spirit of the invention.It should be noted that diagram provided in following embodiment is only to show Meaning mode illustrates basic conception of the invention, and in the absence of conflict, the feature in following embodiment and embodiment can phase Mutually combination.
Wherein, the drawings are for illustrative purposes only and are merely schematic diagrams, rather than pictorial diagram, should not be understood as to this The limitation of invention;Embodiment in order to better illustrate the present invention, the certain components of attached drawing have omission, zoom in or out, not Represent the size of actual product;It will be understood by those skilled in the art that certain known features and its explanation may be omitted and be in attached drawing It is understood that.
The same or similar label correspond to the same or similar components in the attached drawing of the embodiment of the present invention;It is retouched in of the invention In stating, it is to be understood that if there is the orientation or positional relationship of the instructions such as term " on ", "lower", "left", "right", "front", "rear" To be based on the orientation or positional relationship shown in the drawings, be merely for convenience of description of the present invention and simplification of the description, rather than indicate or It implies that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore is described in attached drawing The term of positional relationship only for illustration, is not considered as limiting the invention, for the ordinary skill of this field For personnel, the concrete meaning of above-mentioned term can be understood as the case may be.
The invention discloses a kind of holographic imaging systems of Based on Dual-Aperture diaphragm heterodyne scanning, as shown in Figure 1, including He-Ne Laser source 1, beam expanding lens 2, spectroscope I 3, convex lens I 4, reflective mirror I 5, pupil I 6, AOM acousto-optic modulator 7, reflective mirror II 8, pupil II 9, convex lens II 10, spectroscope II 11, reflective mirror III 12, sample objective table 13, convex lens III 14, light Electric explorer 15, multiplier 16, low-pass filter 17 and computer unit 18.
Wherein, spectroscope and reflective mirror and horizontal platform are in 45° angle, and He-Ne laser source 1 is perpendicular to horizontal platform, 2 water of beam expanding lens It puts down in horizontal platform;
AOM acousto-optic modulator 7 is placed in the middle of spectroscope I 3 and reflective mirror II 8, receives from spectroscope I 3 Light beam, after AOM acousto-optic modulator 7 generate frequency difference light beam pass to reflective mirror II 8;
Photodetector 15 be in surface setting convex lens III 14 focal range in, convex lens III 14 just on Place sample objective table 13 in side;Object under test is placed on sample objective table 13;
Pupil I 6 is placed among reflective mirror I 5 and spectroscope II 11, and pupil II 9 is placed in reflective mirror II 8 and convex lens Between II 10;
Multiplier 16 includes sine multiplier and cosine multiplier;Signal from photodetector 15 passes through multiplier 16 Respectively obtain afterwards optical heterodyne electric signal quadrature component and component in the same direction;Computer unit is entered by low-pass filter 17 again 18。
The invention discloses the detection methods of the holographic imaging systems of above-mentioned Based on Dual-Aperture diaphragm heterodyne scanning, including following step It is rapid:
S1: red blood cell sample is placed on sample objective table, makes cell to be measured in focal range.
S2: starting He-Ne laser source irradiates the beam splitting that light is realized into spectroscope with horizontal direction.
S3: photodetector carries out photoacoustic signal reconstruction by the strength information that detection transmits light, and exposure is arranged Time.
In step sl, the sample objective table is transparent glass container, and glass container inner wall is coated with one layer of use Come weaken photoacoustic waves reflection interference sound-absorbing material, vessel surface coating prevents it from sticking.By red blood cell culture solution according to right Ratio is answered to adjust.
In step s 2, He-Ne laser source wavelength is 632.8nm, power 1.5mW, repetition rate 20Hz.
In step s3, photodetector will measure the light intensity of input, carries out signal reconstruction and passes to stored digital Oscillography unit.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention Art scheme is modified or replaced equivalently, and without departing from the objective and range of the technical program, should all be covered in the present invention Scope of the claims in.

Claims (5)

1. one kind calculates holographic imaging systems based on dicoria heterodyne, it is characterised in that: including He-Ne laser source, beam expanding lens, divide Light microscopic I, convex lens I, reflective mirror I, pupil I, AOM acousto-optic modulator, reflective mirror II, pupil II, convex lens II, spectroscope II, Reflective mirror III, sample objective table, convex lens III, photodetector, multiplier, low-pass filter and computer unit;
Spectroscope I, spectroscope II, reflective mirror I, reflective mirror II and reflective mirror III and horizontal platform angle are 45 °, He-Ne laser source Perpendicular to horizontal platform, beam expanding lens and horizontal platform keep horizontal;
AOM acousto-optic modulator is placed in the midpoint of spectroscope I and reflective mirror II, the light beam from spectroscope I is received, by AOM The different light beam of frequency is generated after acousto-optic modulator, and passes to reflective mirror II;
Photodetector is in the focal range of the convex lens III of surface setting, places sample right above convex lens III Objective table;Object under test is placed on sample objective table;
Pupil I is placed between reflective mirror I and spectroscope II, and pupil II is placed between reflective mirror II and convex lens II;
Multiplier includes sine multiplier and cosine multiplier;Signal from photodetector respectively obtains after multiplier The quadrature component of optical heterodyne electric signal and component in the same direction;Computer unit is entered by low-pass filter again.
2. according to claim 1 a kind of based on dicoria heterodyne calculating holographic imaging systems, it is characterised in that: described He-Ne laser source wavelength is 632.8nm, power 1.5mW, chamber long 250 is mm, repetition rate 20Hz.
3. according to claim 1 a kind of based on dicoria heterodyne calculating holographic imaging systems, it is characterised in that: the light The pupil function p (x, y) of pupil I and pupil II meets Fourier transformation with the laser beam intensity c (x, y) of He-Ne laser source.
4. according to claim 1 a kind of based on dicoria heterodyne calculating holographic imaging systems, it is characterised in that: the sample This objective table is transparent glass container, and glass container inner wall is coated with one layer of sound absorption for being used to weaken the reflection interference of photoacoustic waves Material, glass container surface are covered with the coating for preventing attaching.
5. calculating holographic imaging method, feature based on dicoria heterodyne based on system described in any one of Claims 1 to 4 It is: the described method comprises the following steps:
S1: object under test is placed on sample objective table, and the cell of object under test is made to be maintained at the focal range of convex lens III It is interior;
S2: starting He-Ne laser source irradiates the beam splitting that light is realized into spectroscope I with horizontal direction;
S3: the intensity for the light that photodetector is come in by detection transmission measures, and then carries out signal reconstruction, then be transferred to Computer unit.
CN201910324392.XA 2019-04-22 2019-04-22 One kind calculating holographic imaging systems and method based on dicoria heterodyne Pending CN109884869A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910324392.XA CN109884869A (en) 2019-04-22 2019-04-22 One kind calculating holographic imaging systems and method based on dicoria heterodyne

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910324392.XA CN109884869A (en) 2019-04-22 2019-04-22 One kind calculating holographic imaging systems and method based on dicoria heterodyne

Publications (1)

Publication Number Publication Date
CN109884869A true CN109884869A (en) 2019-06-14

Family

ID=66938018

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910324392.XA Pending CN109884869A (en) 2019-04-22 2019-04-22 One kind calculating holographic imaging systems and method based on dicoria heterodyne

Country Status (1)

Country Link
CN (1) CN109884869A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112947023A (en) * 2021-02-03 2021-06-11 昆明理工大学 Optical scanning holographic three-dimensional object real-time identification system and method
CN113325683A (en) * 2021-05-11 2021-08-31 昆明理工大学 Off-axis scanning holographic three-dimensional target real-time joint transformation recognition system and method

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102920438A (en) * 2012-10-30 2013-02-13 电子科技大学 High-resolution optical scanning holographic slice imaging method based on variable pupils
CN104159094A (en) * 2014-07-09 2014-11-19 四川大学 Method for improving optical scanning holographic tomography effect
CN104614970A (en) * 2015-02-15 2015-05-13 电子科技大学 Optical scanning holographic image edge extracting method based on double-hole pupil
CN106333650A (en) * 2016-09-26 2017-01-18 华南师范大学 Multi-scale photo-acoustic micro-imaging device and multi-scale photo-acoustic micro-imaging method
CN107835074A (en) * 2017-10-16 2018-03-23 电子科技大学 A kind of method for eliminating accidental enciphering optical scanner holography defocus noise
CN108089425A (en) * 2018-01-16 2018-05-29 电子科技大学 A kind of method of the elimination optical scanner holography defocus noise based on deep learning
CN108267945A (en) * 2018-01-16 2018-07-10 电子科技大学 A kind of method of the elimination optical scanner holography defocus noise based on self-organizing map neural network
CN108958000A (en) * 2018-09-17 2018-12-07 电子科技大学 A kind of optical scanner holography self-focusing method based on classification learning and dichotomy
CN109597291A (en) * 2018-11-30 2019-04-09 电子科技大学 A kind of optical scanner hologram image recognition methods based on convolutional neural networks

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102920438A (en) * 2012-10-30 2013-02-13 电子科技大学 High-resolution optical scanning holographic slice imaging method based on variable pupils
CN104159094A (en) * 2014-07-09 2014-11-19 四川大学 Method for improving optical scanning holographic tomography effect
CN104614970A (en) * 2015-02-15 2015-05-13 电子科技大学 Optical scanning holographic image edge extracting method based on double-hole pupil
CN106333650A (en) * 2016-09-26 2017-01-18 华南师范大学 Multi-scale photo-acoustic micro-imaging device and multi-scale photo-acoustic micro-imaging method
CN107835074A (en) * 2017-10-16 2018-03-23 电子科技大学 A kind of method for eliminating accidental enciphering optical scanner holography defocus noise
CN108089425A (en) * 2018-01-16 2018-05-29 电子科技大学 A kind of method of the elimination optical scanner holography defocus noise based on deep learning
CN108267945A (en) * 2018-01-16 2018-07-10 电子科技大学 A kind of method of the elimination optical scanner holography defocus noise based on self-organizing map neural network
CN108958000A (en) * 2018-09-17 2018-12-07 电子科技大学 A kind of optical scanner holography self-focusing method based on classification learning and dichotomy
CN109597291A (en) * 2018-11-30 2019-04-09 电子科技大学 A kind of optical scanner hologram image recognition methods based on convolutional neural networks

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112947023A (en) * 2021-02-03 2021-06-11 昆明理工大学 Optical scanning holographic three-dimensional object real-time identification system and method
CN113325683A (en) * 2021-05-11 2021-08-31 昆明理工大学 Off-axis scanning holographic three-dimensional target real-time joint transformation recognition system and method
CN113325683B (en) * 2021-05-11 2023-01-06 昆明理工大学 Off-axis scanning holographic three-dimensional target real-time joint transformation recognition system and method

Similar Documents

Publication Publication Date Title
EP2592459B1 (en) Holographic microscope and method for processing microscopic subject hologram image
US8731272B2 (en) Computational adaptive optics for interferometric synthetic aperture microscopy and other interferometric imaging
US9581961B2 (en) Holographic microscope, holographic image generation method, and method for acquiring data for holographic image
Verrier et al. Off-axis digital hologram reconstruction: some practical considerations
Kim Principles and techniques of digital holographic microscopy
EP2565725B1 (en) Method and apparatus for the exact reconstruction of the object wave in off-axis digital holography
WO2013040345A1 (en) Systems and methods of dual-plane digital holograghic microscopy
WO2014118325A1 (en) Systems and methods for sub-aperture based aberration measurement and correction in interferometric imaging
Somkuwar et al. Holographic imaging through a scattering layer using speckle interferometry
Das et al. Quantitative phase microscopy using dual-plane in-line digital holography
CN111366557A (en) Phase imaging method based on thin scattering medium
WO2017192896A1 (en) Snapshot optical tomography system and method of acquiring an image with the system
US20220214647A1 (en) Holographic reconstruction apparatus and method
CN109884869A (en) One kind calculating holographic imaging systems and method based on dicoria heterodyne
Badon et al. Distortion matrix concept for deep imaging in optical coherence microscopy
US20180266806A1 (en) Method and apparatus for digital holographic microtomography
Romero et al. Digital holographic microscopy for detection of Trypanosoma cruzi parasites in fresh blood mounts
Monaldi et al. Digital holographic microscopy for microalgae biovolume assessment
Bianco et al. Off‐axis self‐reference digital holography in the visible and far‐infrared region
US20230324275A1 (en) A simple in-line digital holography system for measuring 3d cell shape
Giri et al. Backscatter multiple wavelength digital holography for color micro-particle imaging
Matsuda et al. 3D fluorescence imaging through scattering medium using transport of intensity equation and iterative phase retrieval
CN114184553A (en) Epi-illumination type quantitative phase microscopic device and method based on annular light illumination
Li et al. Efficient framework of solving time-gated reflection matrix for imaging through turbid medium
Goy et al. Imaging thick samples with optical tomography

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190614