CN106124469A - A kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system - Google Patents
A kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system Download PDFInfo
- Publication number
- CN106124469A CN106124469A CN201610481809.XA CN201610481809A CN106124469A CN 106124469 A CN106124469 A CN 106124469A CN 201610481809 A CN201610481809 A CN 201610481809A CN 106124469 A CN106124469 A CN 106124469A
- Authority
- CN
- China
- Prior art keywords
- module
- measured
- biological tissue
- imaging
- optical imagery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003384 imaging method Methods 0.000 title claims abstract description 112
- 230000003287 optical effect Effects 0.000 title claims abstract description 63
- 230000004927 fusion Effects 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 10
- 238000000799 fluorescence microscopy Methods 0.000 claims description 31
- 230000005284 excitation Effects 0.000 claims description 14
- 239000000835 fiber Substances 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 11
- 230000008878 coupling Effects 0.000 claims description 10
- 238000010168 coupling process Methods 0.000 claims description 10
- 238000005859 coupling reaction Methods 0.000 claims description 10
- 238000013480 data collection Methods 0.000 claims description 9
- 238000002604 ultrasonography Methods 0.000 claims description 9
- 101000694017 Homo sapiens Sodium channel protein type 5 subunit alpha Proteins 0.000 claims description 7
- 238000005057 refrigeration Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 206010002091 Anaesthesia Diseases 0.000 claims description 4
- 230000037005 anaesthesia Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 230000004313 glare Effects 0.000 claims description 2
- 238000009434 installation Methods 0.000 claims description 2
- 239000013307 optical fiber Substances 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims description 2
- 238000013139 quantization Methods 0.000 claims description 2
- 238000003325 tomography Methods 0.000 claims description 2
- JNDMLEXHDPKVFC-UHFFFAOYSA-N aluminum;oxygen(2-);yttrium(3+) Chemical compound [O-2].[O-2].[O-2].[Al+3].[Y+3] JNDMLEXHDPKVFC-UHFFFAOYSA-N 0.000 claims 1
- 238000004088 simulation Methods 0.000 claims 1
- 229910019901 yttrium aluminum garnet Inorganic materials 0.000 claims 1
- 230000007812 deficiency Effects 0.000 abstract description 4
- 210000003484 anatomy Anatomy 0.000 abstract description 3
- 230000035790 physiological processes and functions Effects 0.000 abstract description 2
- 210000001519 tissue Anatomy 0.000 description 43
- 239000000523 sample Substances 0.000 description 27
- 230000000694 effects Effects 0.000 description 9
- 238000013170 computed tomography imaging Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 238000002595 magnetic resonance imaging Methods 0.000 description 5
- 238000010895 photoacoustic effect Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 239000003068 molecular probe Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000003444 anaesthetic effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000695 excitation spectrum Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000005865 ionizing radiation Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 210000004872 soft tissue Anatomy 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 229910052779 Neodymium Inorganic materials 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 239000003994 anesthetic gas Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000002223 garnet Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- QEFYFXOXNSNQGX-UHFFFAOYSA-N neodymium atom Chemical compound [Nd] QEFYFXOXNSNQGX-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 229910052727 yttrium Inorganic materials 0.000 description 1
- VWQVUPCCIRVNHF-UHFFFAOYSA-N yttrium atom Chemical compound [Y] VWQVUPCCIRVNHF-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0093—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy
- A61B5/0095—Detecting, measuring or recording by applying one single type of energy and measuring its conversion into another type of energy by applying light and detecting acoustic waves, i.e. photoacoustic measurements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/39—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using tunable lasers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Surgery (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Acoustics & Sound (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system, this system includes: optical imagery module, photoacoustic imaging module, sample carrying locating module, main control module, wherein: sample carrying locating module is used for carrying biological tissue to be measured, is accurately positioned image space；The light path of photoacoustic imaging module and optical imagery module uses " decussation " structure, and the two shared sample carrying locating module, as crossing center；Photoacoustic imaging module for carrying out photoacoustic imaging to biological tissue to be measured, and optical imagery module is for carrying out optical imagery to biological tissue to be measured；Main control module is connected respectively with sample carrying locating module, photoacoustic imaging module, optical imagery module, is controlled with the work schedule to each module, and the view data simultaneously produced image-forming module processes.The present invention can overcome the deficiency of existing single image mode (photoacoustic imaging, optical imagery), reflects more fully anatomical structure and physiological function information.
Description
Technical field
The present invention relates to multi-modal biomedical imaging technical field, particularly relate to a kind of photoacoustic imaging and optical imagery is many
Modality fusion imaging system.
Background technology
Having benefited from the continuous progress of optical molecular probe and imaging means, optical molecular image technology was in more than ten years in past
Obtain development at full speed, and because of its high sensitivity, high specific, receive more to come without ionizing radiation, the feature such as with low cost
The most concerns.Optical molecular probe technique makes the differentiation normal structure of high specific and tissue of interest (such as tumor, blood vessel
Deng) be possibly realized.The most highly sensitive detection molecules probe distribution situation in biology and then reaction organism physiology, disease
Reason information is a major issue of iconography research.Owing to biological tissue has the strongest absorption and scattering process to photon,
The degree of depth causing optical imagery is limited, and the spatial resolution ratio that optical 3-dimensional is rebuild is relatively low.How improve raising optical imagery
Imaging depth and the precision of three-dimensional reconstruction, be optical molecular image problem demanding prompt solution.To this end, phase between photon with material
Two kinds of physical effect fluorescent effects of interaction and optoacoustic effect are fully excavated so that people can " see "
" hear " interaction between light and biological tissue, molecular probe.Fluorescent effect refers to when high-energy short wavelength photons is penetrated
When entering Cucumber, the Electron absorption energy in material, from ground state transition to high level；Due to shakiness when electronics is in high level
Fixed, low-lying level will be transitted to from high level, thus give off energy, send longer wavelengths of fluorescent photon.With fluorescent effect
It is different that middle material discharges, with the form of photon radiation, the energy absorbed, the energy in optoacoustic effect, on photon irradiation to material
Amount, is converted to heat energy, and then is converted into mechanical vibration, discharges, with the form of ultrasound wave, the energy absorbed.By fluorescent effect
And optoacoustic effect, grow up the most concerned fluorescence imaging and photoacoustic imaging both image modes respectively.
Imaging-PAM occupies an important position in the development of molecular image.Fluorescent molecules imaging
(fluorescence molecular imaging, FMT), it is possible to achieve the three-dimensional reconstruction of fluorescence signal.Due to fluorescent photon
High scattering properties in vivo, the spatial resolution ratio causing fluorescence imaging is relatively low, and three-dimensional reconstruction also has the strongest disease
State property.To this end, a lot of research worker introduce other image modes to make up the deficiency of Imaging-PAM.By X-ray CT imaging with
FMT imaging technique merges, it is possible to use the high-resolution organism anatomical structure that CT imaging technique provides carries as prior information
The three-dimensional reconstruction quality of high fluorescence signal.Although but CT imaging can provide high-resolution structural information, during CT imaging not
Evitable there is ionizing radiation, and the resolution of soft tissue is compared relatively low by CT imaging.Except CT imaging, magnetic resonance becomes
Anatomical information is provided into optical 3-dimensional imaging as (Magnetic Resonance Imaging, MRI) can also be used for.MRI
Imaging is possible not only to provide the soft tissue resolution of high-contrast, may be provided for the functional metabolism information of organism simultaneously.But
Being optical image technology and MRI imaging to be combined, need to produce the magnetic field of superelevation field intensity, this causes the volume ratio of imaging device
Relatively big, equipment cost is higher, thus limits the development of this multi-modal fusion mode.Although it is pointed out that CT and
MRI can provide the anatomical information of organism, but they the optical parametric information of organism that all cannot directly provide
(absorptance of biological tissue and scattering coefficient).Other image mode how is utilized to obtain the optical specificity letter of biological tissue
Breath, thus improving fluorescent three-dimensional imaging effect is a good problem to study.
Different from fluorescence imaging, in photoacoustic imaging, what imaging system detected is the ultrasound wave of optoacoustic effect generation, passes through
Rebuild the initial sonic pressure field of optoacoustic effect thus reflect the optical absorption characteristic of biological tissue.Owing to ultrasound wave is in biological tissue
The scattering coefficient three number magnitude less than the scattering coefficient of photon propagated, therefore, it can be prevented effectively from fluorescence imaging glimmering
The various problems that the scattering in biological tissues of light photon brings.Additionally, due to the wavelength of ultrasound wave is smaller, therefore can obtain
The mechanics of biological tissue information of high spatial resolution.Photoacoustic imaging combines high contrast features and the ultra sonic imaging of optical imagery
High spatial resolution characteristic.Photoacoustic imaging is combined with fluorescence imaging, it is possible to use photoacoustic imaging is fluorescent three-dimensional imaging
Organism optical specificity information is provided, thus is effectively improved the imaging effect that fluorescent three-dimensional is rebuild.Owing to sound wave is in biological tissue
Propagation attenuation much smaller than optical signal, so photoacoustic imaging has deeper imaging depth.In conjunction with multispectral photoacoustic imaging technology,
Or use arc ultrasound transducer array, it is possible to achieve toy whole body photoacoustic imaging.Melting of fluorescence and photoacoustic imaging technology
This difficult problem of optical imagery being combined into Form provides a kind of feasible solution.
Summary of the invention
Present invention aim to overcome that existing single photoacoustic imaging and the deficiency of single Optical imaging modalities, reflection is more comprehensively
Anatomical structure and physiological function information.
For reaching above-mentioned purpose, the technical scheme is that a kind of photoacoustic imaging and optical imagery are multi-modal
Fusion of imaging system, this system includes: optical imagery module, photoacoustic imaging module, sample carrier module, main control module, wherein:
Described sample carrying locating module is used for carrying biological tissue to be measured；
Described photoacoustic imaging module includes photo-acoustic excitation light source and photoacoustic signal detection module, is symmetrically positioned in sample carrying mould
Photoacoustic imaging light is formed between the both sides of block, and described photo-acoustic excitation light source, biological tissue to be measured and photoacoustic signal detection module
Road；Described photo-acoustic excitation light source sends pulse laser and exposes in described biological tissue to be measured, described photoacoustic signal detection mould
Block for carrying out ultrasonic signal collection to described biological tissue to be measured, and the ultrasound wave analogue signal received is converted into number
Word voltage signal, to realize the imaging of biological tissue to be measured；
Described optical imagery module includes fluorescence imaging module light source and fluorescence imaging module data collection device, is symmetrically positioned in
The both sides of sample carrier module, and described fluorescence imaging module light source, biological tissue to be measured and fluorescence imaging module data collection
Optical imagery light path is formed between device；Described fluorescence imaging module light source sends continuous laser, for being placed on sample carrying
The fluorescent material within biological tissue to be measured in module excites, and described fluorescence imaging module data collection device is used for gathering
The fluorescence signal being excited inside described biological tissue to be measured, to generate multispectral image；
Described photoacoustic imaging light path and optical imagery light path use " decussation " structure, and the two shares the carrying of described sample
Module, as crossing center；Described photoacoustic imaging module is for carrying out photoacoustic imaging, described optics to described biological tissue to be measured
Image-forming module is for carrying out optical imagery to described biological tissue to be measured；
Described main control module is connected respectively with described sample carrier module, photoacoustic imaging module, optical imagery module, with right
Described sample carrier module, photoacoustic imaging module, the work schedule of optical imagery module are controlled, and to described photoacoustic imaging
The view data that module and optical imagery module produce processes.The present invention compared with prior art has the advantage that
1, the present invention provide a kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system, single mould can be overcome
The deficiency of state image-forming information, it is provided that more fully organizational structure and function information.
2, the imaging system that the present invention provides can be reached to circulate the effect of lifting mutually by modeling, obtained more accurately
Registration fusion image.
3, the imaging system that the present invention provides can carry out toy whole body three-dimensional imaging.
Accompanying drawing explanation
Fig. 1 is the fusion optoacoustic according to one embodiment of the invention and Optical multi-mode state imaging system population structure schematic diagram；
Fig. 2 is the imaging system photoacoustic imaging modular structure schematic diagram according to one embodiment of the invention；
Fig. 3 is the imaging system fluorescence imaging modular structure schematic diagram according to one embodiment of the invention；
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with specific embodiment, and reference
Accompanying drawing, the present invention is described in more detail.
Fig. 1 is that the structure of the photoacoustic imaging according to one embodiment of the invention and optical imagery multi-modal fusion imaging system is shown
It is intended to, as it is shown in figure 1, described photoacoustic imaging and optical imagery multi-modal fusion imaging system include: sample carrier module, optoacoustic
Image-forming module, optical imagery module and main control module, wherein:
Described sample carrying locating module is used for carrying biological tissue to be measured；It includes for fixing described biological group to be measured
The unobstructed scanning fixed support device knitted, for the anesthesia outfit providing described biological tissue to be measured to anaesthetize, for driving
That states sample carrier module vertically moves the sample positioning device that sample i.e. carries out height adjustment and horizontal rotation translation etc., makes
Obtain described sample carrier module can carry out horizontally rotating and vertical displacement movement, and provide life to maintain for biological tissue to be measured
Environment, drives biological tissue to be measured to move according to certain program.In order to described biological tissue to be measured is placed in imaging storehouse, water
Flat turntable uses and hangs upside down fixed installation.Toy is carried out imaging in vivo when, toy is automatic
Image quality can be impacted by physiological movement, and in order to avoid the generation of this situation, the present invention have also been devised special petty action
Thing fixed support, by fixing the positions such as toy extremity, head, reduces the width of toy physiological movement to the full extent
Degree so that toy keeps form not change in imaging process, in order to registrate with the information fusion in later stage.
Wherein, described anesthesia outfit can be gas anesthesia device, including anesthetic machine, anesthetic gases and anaesthetic mask.
Described photoacoustic imaging module includes photo-acoustic excitation light source and photoacoustic signal detection module, is symmetrically positioned in sample carrying mould
Photoacoustic imaging light is formed between the both sides of block, and described photo-acoustic excitation light source, biological tissue to be measured and photoacoustic signal detection module
Road；Described photo-acoustic excitation light source sends pulse laser and exposes in described biological tissue to be measured, to increase the degree of depth of imaging, and institute
State photoacoustic signal detection module for described biological tissue to be measured is carried out ultrasonic signal collection, and the ultrasound wave that will receive
Analogue signal is converted into digital voltage signal, to realize the fault imaging of biological tissue to be measured.
Described optical imagery module includes fluorescence imaging module light source and fluorescence imaging module data collection device, is symmetrically positioned in
The both sides of sample carrier module, and described fluorescence imaging module light source, biological tissue to be measured and fluorescence imaging module data collection
Optical imagery light path is formed between device；Described fluorescence imaging module light source sends continuous laser, for being placed on sample carrying
The fluorescent material within biological tissue to be measured in module excites, and described fluorescence imaging module data collection device is used for gathering
The fluorescence signal being excited inside described biological tissue to be measured, to generate multispectral image.
Described photoacoustic imaging light path and optical imagery light path use " decussation " structure, and the two shares the carrying of described sample
Module, as crossing center；Described photoacoustic imaging module is for carrying out photoacoustic imaging, described optics to described biological tissue to be measured
Image-forming module is for carrying out optical imagery to described biological tissue to be measured.
Described main control module is connected respectively with described sample carrier module, photoacoustic imaging module, optical imagery module, with right
Described sample carrier module, photoacoustic imaging module, the work schedule of optical imagery module are controlled, and to described photoacoustic imaging
The view data that module and optical imagery module send processes, and described process includes the reconstruction of optoacoustic and fluoroscopic image, with
And rebuild the fusion registration of image.
Fig. 2 is the structural representation of the photoacoustic imaging module according to one embodiment of the invention, as in figure 2 it is shown, described optoacoustic
Excitation source module includes: pulse laser, tunable pulsed laser device and fiber coupling device, described photoacoustic signal detection mould
Block includes focused transducer, data acquisition unit, mechanical framework, wherein:
Described pulse laser, for providing pumping source for rear class tunable pulsed laser device, in one embodiment, uses
The neodymium doped yttrium aluminium garnet laser (Q-switch Nd:YAG laser) adjusting Q provides excitaton source laser pulse, and it is a kind of
Nanosecond solid state laser, single pulse energy is up to hundreds of MJ；
Described tunable pulsed laser device is driven by prime pulse laser, it is provided that wavelength and the most adjustable pulse of energy
Laser exports, for multispectral photoacoustic imaging, in an embodiment of the present invention, and the pulse laser of described Wavelength tunable adjustable
Harmonic wave section is 680nm～960nm；
Described fiber coupling device is positioned at the rear class of described tunable pulsed laser device, and it uses two bundle multimode fibres,
After having coupled the pulse laser output of described tunable pulsed laser device, it is irradiated to be placed on sample from two relative directions and holds
Carry the biological tissue to be measured in module, to increase the degree of depth of imaging；In one embodiment, described multimode fibre is for using multi beam list
The optical fiber that mode fiber is rolled into；And described multimode fibre is being single fiber end face close to the one end at laser exit,
Placing coupled lens therebetween, the other end is the outlet fiber end face of multiple beam splitting (such as 10), its coupling pulse laser from
Focused transducer upper and lower surface is irradiated in described biological tissue to be measured at a certain angle, to increase the degree of depth of imaging.
Described focused transducer is positioned at the rear of described sample carrier module, by vertically moving described life to be measured
Fabric texture carries out the scanning of different tomography, carry out ultrasonic signal collection for described biological tissue to be measured, and will receive
Ultrasound wave analogue signal is converted to digital voltage signal, to realize the fault imaging of biological tissue to be measured.Imaging depth, signal to noise ratio
The problem needing when being all select ultrasonic transducer with image resolution ratio to consider, some important parameters of ultrasonic transducer include:
Sensitivity, mid frequency, bandwidth, probe size, array number and shape etc..In an embodiment of the present invention, 128 times can be used
The arcs of recesses focused transducer of unit, mid frequency is 5MHz；
Described data acquisition unit is positioned at the final stage of described photoacoustic imaging module, and it is for by described focused transducer
The ultra-weak electronic signal being converted to is amplified and quantifies, and will amplify and quantify after electrical signal data be transferred to main control module
Carry out follow-up data to process；In an embodiment of the present invention, the sample rate of described data acquisition unit is 50Mps, quantization digit
It it is 12；
Described mechanical framework includes the water tank for holding coupling liquid, for fixing the fixed support of described ultrasonic transducer
In device.
Fig. 3 is the structural representation of the optical imagery module according to one embodiment of the invention, as it is shown on figure 3, described fluorescence
Image-forming module light source includes: continuous-wave laser and condenser lens；Described fluorescence imaging module harvester includes narrow band filter slice
With cryogenic refrigeration ICCD camera, wherein:
The continuous laser of fixed wave length launched by described continuous-wave laser, for being placed on described sample carrier module
The fluorescent material within biological tissue to be measured excite；Owing to each fluorescent material has the excitation spectrum of oneself and sends out
Penetrate spectrum, excite fluorescence in order to more efficiently produce, in one embodiment, select wavelength at fluorescent material excitation spectrum
Laser instrument near peak wavelength is as excitation source, therefore, in order to carry out the excitation experiment of different fluorescent material, often
Need the continuous-wave laser of multi-wavelength；
In existing FMT algorithm for reconstructing, all excitation source is regarded as the skin next one transmission free path position each to
The point source of the same sex, in order to reach such effect, needs to focus on the exciting light incided in biological tissue to be measured
On one point as far as possible the least, and the effect being positioned at the described condenser lens of described continuous-wave laser rear class be exactly by described continuously
The continuous laser that wave laser sends focuses on a point in the biological tissue to be measured being placed on sample carrier module；
Described narrow band filter slice is positioned at before cryogenic refrigeration CCD camera camera lens, is used for filtering described continuous-wave laser
Veiling glare in the exciting light of output and environment.
Described cryogenic refrigeration ICCD camera is positioned at the final stage of described optical imagery module, and it is for biological tissue to be measured quilt
The fluorescence excited carries out signals collecting；Excite fluorescence to be typically the faintest, need the ICCD camera using cryogenic refrigeration
Carry out signals collecting, such as can use the ICCD camera of liquid nitrogen refrigerating or the ICCD camera of semiconductor refrigerating.
During described system imaging, carry out photoacoustic imaging first with described photoacoustic imaging module, then by biological group to be imaged
Knit and rise to waterborne, utilize described optical imagery module to carry out fluorescence imaging, then by photoacoustic imaging data and fluorescence imaging number
According to being transferred to main control module, the image of the two is registrated, the information superposition of same position is shown.
In one embodiment of the invention, photoacoustic imaging completes under water, it is ensured that the photoacoustic signal that tissue produces transmits from body surface
Constant to this segment distance velocity of sound of detector.Optical imagery is to complete on horizontal plane.In a particular application, first carry out under water
The signals collecting of the different aspects of photoacoustic signal, is raised to carry out on horizontal plane the collection of optical signalling afterwards.Described photoacoustic imaging
And the light path decussation of optical imagery be for later stage both modalities which between registration, in this manner, registration can compare
Easily.
Particular embodiments described above, has been carried out the purpose of the present invention, technical scheme and beneficial effect the most in detail
Describe in detail bright, be it should be understood that the specific embodiment that the foregoing is only the present invention, be not limited to the present invention, all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. done, should be included in the guarantor of the present invention
Within the scope of protecting.
Claims (9)
1. a photoacoustic imaging and optical imagery multi-modal fusion imaging system, it is characterised in that this system includes: optical imagery
Module, photoacoustic imaging module, sample carrier module, main control module, wherein:
Described sample carrying locating module is used for carrying biological tissue to be measured；
Described photoacoustic imaging module includes photo-acoustic excitation light source and photoacoustic signal detection module, is symmetrically positioned in sample carrier module
Photoacoustic imaging light path is formed between both sides, and described photo-acoustic excitation light source, biological tissue to be measured and photoacoustic signal detection module；Institute
Stating photo-acoustic excitation light source send pulse laser and expose in described biological tissue to be measured, described photoacoustic signal detection module is used for
Described biological tissue to be measured is carried out ultrasonic signal collection, and the ultrasound wave analogue signal received is converted into digital voltage
Signal, to realize the imaging of biological tissue to be measured；
Described optical imagery module includes fluorescence imaging module light source and fluorescence imaging module data collection device, is symmetrically positioned in sample
The both sides of carrier module, and described fluorescence imaging module light source, biological tissue to be measured and fluorescence imaging module data collection device it
Between formed optical imagery light path；Described fluorescence imaging module light source sends continuous laser, for being placed on sample carrier module
On the fluorescent material within biological tissue to be measured excite, described fluorescence imaging module data collection device is used for gathering described
The fluorescence signal being excited inside biological tissue to be measured, to generate multispectral image；
Described photoacoustic imaging light path and optical imagery light path use " decussation " structure, and the two shares described sample carrying mould
Block, as crossing center；Described photoacoustic imaging module is for carrying out photoacoustic imaging to described biological tissue to be measured, and described light studies
As module is for carrying out optical imagery to described biological tissue to be measured；
Described main control module is connected respectively with described sample carrier module, photoacoustic imaging module, optical imagery module, with to described
Sample carrier module, photoacoustic imaging module, the work schedule of optical imagery module are controlled, and to described photoacoustic imaging module
The view data produced with optical imagery module processes.
System the most according to claim 1, it is characterised in that described sample carrier module includes for fixing described to be measured
The unobstructed scanning fixed support device of biological tissue, for provide described biological tissue to be measured gas anesthesia device and
For being responsible for described biological tissue to be measured height and position and the sample positioning device of horizontal rotation angle adjustment, described horizontal rotation
Platform uses and hangs upside down fixed installation.
System the most according to claim 1, it is characterised in that described view data carries out process and includes multispectral optoacoustic
With the reconstruction of fluoroscopic image, and multispectral optoacoustic and fluoroscopic image registration merge.
System the most according to claim 1, it is characterised in that described photo-acoustic excitation light source includes: pulse laser, adjustable
Humorous pulse laser and fiber coupling device；Described photoacoustic signal detection module includes focused transducer and data acquisition
Device, wherein:
Described pulse laser is for providing pumping source for rear class tunable pulsed laser device；
Described tunable pulsed laser device is driven by described pulse laser, it is provided that wavelength and the most adjustable pulse laser of energy
Output, for multispectral photoacoustic imaging；
Described fiber coupling device is positioned at the rear class of described tunable pulsed laser device, uses two bundle multimode fibres, is coupling
After the pulse laser output of described tunable pulsed laser device, it is irradiated to be placed on sample carrier module from two relative directions
On biological tissue to be measured, to increase the degree of depth of imaging；
Described focused transducer is positioned at the rear of described sample carrier module, by vertically moving described biological group to be measured
Knit the scanning carrying out different tomographies, ultrasonic signal collection is carried out for described biological tissue to be measured, and ultrasonic by receive
Wave simulation signal is converted to digital voltage signal, to realize the fault imaging of biological tissue to be measured；
Described data acquisition unit is positioned at the final stage of described photoacoustic imaging module, for being changed by described focused transducer
To weak voltage signals be amplified and quantify, and the voltage signal data after amplifying and quantify is transferred to main control module and enters
The data process that row is follow-up.
System the most according to claim 4, it is characterised in that described pulse laser is the neodymium-doped yttrium-aluminum garnet adjusting Q
Laser instrument；And/or
The tunable wave band of the pulse laser that described tunable pulsed laser device provides is 680nm～960nm；And/or
Described ultrasonic transducer is the arcs of recesses focused transducer of 128 array elements, and mid frequency is 5MHz；And/or
The sample rate of described data acquisition unit is 50Mps, and quantization digit is 12.
System the most according to claim 4, it is characterised in that described multimode fibre is rolled into for using multi beam single-mode fiber
An optical fiber；And described multimode fibre is being single fiber end face close to the one end at laser exit, place coupling therebetween
Lens, the other end is the outlet fiber end face of multiple beam splitting, its coupling pulse laser from focused transducer upper and lower surface with
Certain angle is irradiated in described biological tissue to be measured.
System the most according to claim 4, it is characterised in that described photoacoustic imaging module also includes mechanical framework, described
Mechanical framework includes the water tank for holding coupling liquid and for fixing the fixing support rack of described focused transducer.
8. according to the system described in any one of claim 1-7, it is characterised in that described fluorescence imaging module light source includes: even
Continuous wave laser and condenser lens；Described fluorescence imaging module harvester includes narrow band filter slice and cryogenic refrigeration ICCD camera,
Wherein: the continuous laser of fixed wave length launched by described continuous-wave laser, for being placed on described sample carrier module
Fluorescent material within biological tissue to be measured excites；
Described condenser lens is positioned at the rear class of described continuous-wave laser, swashs continuously for sent by described continuous-wave laser
Light focuses on the precalculated position in the biological tissue to be measured being placed on described sample carrier module；
Described narrow band filter slice is positioned at before cryogenic refrigeration CCD camera camera lens, is used for filtering the output of described continuous-wave laser
Exciting light and environment in veiling glare；
Described cryogenic refrigeration ICCD camera is positioned at the final stage of described optical imagery module, and it is for being excited to biological tissue to be measured
Fluorescence carry out signals collecting.
System the most according to claim 1, it is characterised in that when imaging, utilizes described acoustic imaging module pair under water
Described biological tissue to be measured carries out photoacoustic imaging, then rises to waterborne by described biological tissue to be measured, utilizes described light to study
As module carries out fluorescence imaging, then photoacoustic imaging data and fluorescence imaging data are transferred to main control module, the figure to the two
As registrating, the information superposition of same position is shown.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610481809.XA CN106124469A (en) | 2016-06-27 | 2016-06-27 | A kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610481809.XA CN106124469A (en) | 2016-06-27 | 2016-06-27 | A kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106124469A true CN106124469A (en) | 2016-11-16 |
Family
ID=57266441
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610481809.XA Pending CN106124469A (en) | 2016-06-27 | 2016-06-27 | A kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106124469A (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107049549A (en) * | 2017-05-31 | 2017-08-18 | 杭州高能医疗设备有限公司 | The Machinery Control System of imaging |
| CN107485408A (en) * | 2017-09-13 | 2017-12-19 | 北京大学 | A kind of double-mode imaging system and its imaging method for realizing XCT and photoacoustic imaging |
| CN108344697A (en) * | 2018-05-14 | 2018-07-31 | 苏州大学 | A kind of special loading shelf combination of the external sample of optoacoustic tomoscan molecular imaging system |
| CN108451507A (en) * | 2018-04-28 | 2018-08-28 | 国家纳米科学中心 | Probe in detecting changing device and toy optoacoustic tomographic system |
| CN108717045A (en) * | 2018-06-05 | 2018-10-30 | 哈尔滨工业大学（威海） | A kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence |
| CN109009182A (en) * | 2018-06-05 | 2018-12-18 | 南方医科大学 | A kind of method and registration apparatus for realizing that optoacoustic and his mode are serially imaged |
| CN109781241A (en) * | 2019-02-01 | 2019-05-21 | 华南师范大学 | A kind of device and method based on optoacoustic effect high-acruracy survey ultrasound field distribution |
| WO2019119576A1 (en) * | 2017-12-22 | 2019-06-27 | 深圳先进技术研究院 | Multi-mode imaging system |
| CN110151142A (en) * | 2019-06-27 | 2019-08-23 | 刘新新 | A kind of medical multi-modal hybrid image forming apparatus of integration |
| CN110261315A (en) * | 2019-06-27 | 2019-09-20 | 同济大学 | A kind of scanning near-field opto-acoustic microscopic imaging instrument |
| CN110530797A (en) * | 2019-09-25 | 2019-12-03 | 南京大学 | A kind of the double-mode imaging device and its application method of optoacoustic ghost imaging-fluorescence imaging |
| CN111665295A (en) * | 2020-05-28 | 2020-09-15 | 深圳第三代半导体研究院 | Solid material and semiconductor device internal defect detection method |
| CN112617904A (en) * | 2021-01-08 | 2021-04-09 | 中国科学技术大学 | Three-dimensional panoramic fault ultrasonic device |
| CN114209282A (en) * | 2021-12-22 | 2022-03-22 | 复旦大学 | Ultrasonic and photoacoustic multi-mode high-resolution three-dimensional scanning imaging device |
| US11786128B2 (en) * | 2020-06-18 | 2023-10-17 | Illumisonics Inc. | PARS imaging methods |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785662A (en) * | 2010-03-09 | 2010-07-28 | 华南师范大学 | Bimodal system and method integrating photoacoustic imaging and fluorescence imaging |
| CN102499645A (en) * | 2011-11-08 | 2012-06-20 | 西安电子科技大学 | Photoacoustic and fluorescence dual-mode integrated tomography imaging system and imaging method |
| CN103389273A (en) * | 2013-08-01 | 2013-11-13 | 中国科学院自动化研究所 | Photo-acoustic and optical integrated multi-mode imaging system |
-
2016
- 2016-06-27 CN CN201610481809.XA patent/CN106124469A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785662A (en) * | 2010-03-09 | 2010-07-28 | 华南师范大学 | Bimodal system and method integrating photoacoustic imaging and fluorescence imaging |
| CN102499645A (en) * | 2011-11-08 | 2012-06-20 | 西安电子科技大学 | Photoacoustic and fluorescence dual-mode integrated tomography imaging system and imaging method |
| CN103389273A (en) * | 2013-08-01 | 2013-11-13 | 中国科学院自动化研究所 | Photo-acoustic and optical integrated multi-mode imaging system |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107049549A (en) * | 2017-05-31 | 2017-08-18 | 杭州高能医疗设备有限公司 | The Machinery Control System of imaging |
| CN107049549B (en) * | 2017-05-31 | 2019-04-09 | 杭州高能医疗设备有限公司 | The Machinery Control System of imaging |
| CN107485408A (en) * | 2017-09-13 | 2017-12-19 | 北京大学 | A kind of double-mode imaging system and its imaging method for realizing XCT and photoacoustic imaging |
| WO2019119576A1 (en) * | 2017-12-22 | 2019-06-27 | 深圳先进技术研究院 | Multi-mode imaging system |
| CN108451507A (en) * | 2018-04-28 | 2018-08-28 | 国家纳米科学中心 | Probe in detecting changing device and toy optoacoustic tomographic system |
| CN108344697A (en) * | 2018-05-14 | 2018-07-31 | 苏州大学 | A kind of special loading shelf combination of the external sample of optoacoustic tomoscan molecular imaging system |
| CN108344697B (en) * | 2018-05-14 | 2024-02-09 | 苏州大学 | Special sample loading frame combination for in-vitro samples of photoacoustic tomography molecular imaging system |
| CN108717045A (en) * | 2018-06-05 | 2018-10-30 | 哈尔滨工业大学（威海） | A kind of ultrasonic, three modality imaging system of optoacoustic and fluorescence |
| CN109009182B (en) * | 2018-06-05 | 2021-08-27 | 南方医科大学 | Method for realizing serial imaging of optoacoustic and other modes and registration device |
| CN109009182A (en) * | 2018-06-05 | 2018-12-18 | 南方医科大学 | A kind of method and registration apparatus for realizing that optoacoustic and his mode are serially imaged |
| CN109781241A (en) * | 2019-02-01 | 2019-05-21 | 华南师范大学 | A kind of device and method based on optoacoustic effect high-acruracy survey ultrasound field distribution |
| CN110151142A (en) * | 2019-06-27 | 2019-08-23 | 刘新新 | A kind of medical multi-modal hybrid image forming apparatus of integration |
| CN110261315A (en) * | 2019-06-27 | 2019-09-20 | 同济大学 | A kind of scanning near-field opto-acoustic microscopic imaging instrument |
| CN110530797A (en) * | 2019-09-25 | 2019-12-03 | 南京大学 | A kind of the double-mode imaging device and its application method of optoacoustic ghost imaging-fluorescence imaging |
| CN111665295A (en) * | 2020-05-28 | 2020-09-15 | 深圳第三代半导体研究院 | Solid material and semiconductor device internal defect detection method |
| US11786128B2 (en) * | 2020-06-18 | 2023-10-17 | Illumisonics Inc. | PARS imaging methods |
| CN112617904A (en) * | 2021-01-08 | 2021-04-09 | 中国科学技术大学 | Three-dimensional panoramic fault ultrasonic device |
| CN114209282A (en) * | 2021-12-22 | 2022-03-22 | 复旦大学 | Ultrasonic and photoacoustic multi-mode high-resolution three-dimensional scanning imaging device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106124469A (en) | A kind of photoacoustic imaging and optical imagery multi-modal fusion imaging system | |
| CN103389273A (en) | Photo-acoustic and optical integrated multi-mode imaging system | |
| US8454512B2 (en) | Confocal photoacoustic microscopy with optical lateral resolution | |
| CN102822661B (en) | Opto-acoustic imaging devices and acousto-optic imaging method | |
| CN104188625B (en) | A kind of multi-modal micro imaging system | |
| US10299685B2 (en) | Method and apparatus to enhance light illuminating intensity and diffusivity | |
| CN107607473B (en) | Simultaneous multipoint excitation and matching received photoacoustic three-dimensional imaging device and method | |
| CN105595964B (en) | Double focusing ultrasonic probe and thinned array Photoacoustic tomography system | |
| JP5911196B2 (en) | Photoacoustic imaging device | |
| JP2010088873A (en) | Biological information imaging apparatus | |
| US20130190594A1 (en) | Scanning Optoacoustic Imaging System with High Resolution and Improved Signal Collection Efficiency | |
| CN104825180A (en) | Tri-modal breast imaging system and imaging method thereof | |
| CN101336832A (en) | Pulse type optical acoustic scanning soft-tissue imaging method and device | |
| CN103393407B (en) | Handheld photoacoustic imaging probe | |
| US20090069695A1 (en) | Device for imaging a turbid medium | |
| CN108553080A (en) | A kind of stereoscan optoacoustic Jie's sight imaging system towards toy subcutaneous tumor | |
| CN103313649A (en) | Measuring apparatus | |
| CN103393406B (en) | Simple and easy hand-held photoacoustic imaging probe | |
| CN103385697A (en) | High-performance handheld photoacoustic imaging probe | |
| van Raaij et al. | Femtosecond photoacoustics: integrated two-photon fluorescence and photoacoustic microscopy | |
| JP2014144109A (en) | Subject information acquisition device | |
| Kosik et al. | Combined 3D photoacoustic and 2D fluorescence imaging of indocyanine green contrast agent flow | |
| He et al. | In vivo imaging of a single erythrocyte with high-resolution photoacoustic microscopy | |
| Lin et al. | Real-time co-registered photoacoustic and ultrasonic imaging for early endometrial cancer detection driven by cylindrical diffuser | |
| CN112415096B (en) | Super-resolution photoacoustic imaging system and method based on saturable absorption effect |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161116 |