CN102499645A - Photoacoustic and fluorescence dual-mode integrated tomography imaging system and imaging method - Google Patents
Photoacoustic and fluorescence dual-mode integrated tomography imaging system and imaging method Download PDFInfo
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Abstract
The invention discloses a photoacoustic and fluorescence dual-mode integrated tomography imaging system and a photoacoustic and fluorescence dual-mode integrated tomography imaging method. The system comprises a modulation laser light source (1), a vibrating mirror (2), a sample rotary table (3), a filtering wheel (4), a gating intensified charge-coupled device (ICCD) camera (5), a photoacoustic detection acquisition module (6), a system control module (7), a computer (8), a data bus (9) and a control bus (10), wherein the vibrating mirror, the sample rotary table, the filtering wheel and the gating ICCD camera are positioned on the same straight line with the direction of lasers; the ultrasound transduction array normal direction of the photoacoustic detection acquisition module is perpendicular to the straight line to form a crossed vertical detection mode. According to the imaging method, light absorption distribution formed by photoacoustic tomography imaging is used as prior information for the reestablishment of fluorescence tomography imaging, and the positions of fluorophores, fluorescent yield and fluorescence lifetime parameters are reestablished by an iterative method. By the photoacoustic and fluorescence dual-mode integrated tomography imaging system and the photoacoustic and fluorescence dual-mode integrated tomography imaging method, the imaging complexity can be reduced, the cost of hardware can be saved, and the flexibility of the system and the reestablishing accuracy of the fluorescence tomography imaging can be improved.
Description
Technical field
The invention belongs to the image system technical field, particularly a kind of optoacoustic and fluorescent dual module integral computed tomography (SPECT) system can carry out high accuracy to light absorption parameter, fluorescence light source position, fluorescent yield and fluorescence lifetime in the complex biological body and rebuild.Unusual for detecting in the pathological process cell and molecular level, explore that tumor takes place, development and shifting, the monitor treatment process is estimated curative effect of medication effective means is provided.
Background technology
The optoacoustic fault imaging is a kind of harmless medical imaging method that developed recently gets up, and it has combined the high contrast features of pure optical imagery and the high-penetration depth characteristic of pure ultra sonic imaging, and the imaging of tissue of high-resolution and high-contrast can be provided.The optoacoustic fault imaging promptly utilizes the pulse laser of nanometer pulsewidth or the continuous wave laser irradiation sample of amplitude modulation(PAM) based on optoacoustic effect, and sample tissue absorbs luminous energy; The transform light energy that partially absorbs becomes heat energy; Make and organize expanded by heating, thereby tissue is on every side produced extruding, this extruding blazes abroad with hyperacoustic form; Utilize the ultrasonic detector collection to propagate into the external photoacoustic signal of sample and rebuild, obtain light absorption distribution in the sample body.Can be used for research fields such as cardiovascular disease, drug metabolism, gene expression, tumor, just progressively become the research focus of biological tissue's technical field of nondestructive testing.
The fluorescence fault imaging is nearly ten years a kind of novel molecules that grow up, the analysis and detection technology of gene expression.This imaging mode has been considered light in intravital absorption of sample and scattering, through measuring samples body surface photon stream density, utilizes mathematical method to reconstruct the intravital fluorescent material of tissue sample and distributes and concentration, and be a kind of relatively accurate, quantitative analytical method.According to the difference of laser works pattern, the fluorescence fault imaging can be divided into three types of continuous wave, frequency domain and time domains.These three types of imaging system costs from low to high, can obtain quantity of information is that continuous wave mode is minimum, the time-domain and frequency-domain pattern is identical.Frequency domain fluorescence fault imaging adopts the continuous wave laser of amplitude modulation(PAM) as irradiation source, adopts the time resolution detection mode to gather fluorescence signal and is used for rebuilding, thereby obtain fluorogen or fluorescent probe position, fluorescent yield and fluorescence lifetime in the body.The fluorescence fault imaging can carry out in research body, non-intrusion type people to problems such as mutual, the cell activities between gene expression, protein function, the protein, more and more receives people's attention.
Because boundary survey information is limited, the fluorescence tomography rebuilding belongs to ill-conditioning problem.The light absorption that utilizes the optoacoustic fault imaging to obtain distributes as the prior information of fluorescence tomography rebuilding, can improve light source location and quantitative accuracy.Referring to D.Razansky, " Hybrid photoacoustic fluorescence molecular tomography using finite-element based inversion ", Medical Physics, 34:4293-301,2007.Adopt two to overlap independently imaging system and carry out two kinds of image modes imagings respectively in the document, living small animal form, posture unavoidably change in the imaging process, have increased the reconstruction difficulty.Existing optoacoustic computed tomography (SPECT) system, places water tank to carry out the acoustic impedance coupling imaging object, and adopts the contact detection mode as excitation source with pulse laser, obtains biological tissue's light absorption and distributes.Referring to X.D Wang; " Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain "; Nature Biology, 21 (7): 803-806,2003 and L.Z.Xiang; " Real-time optoacoustic monitoring of vascular damage during photodynamic therapy treatment of tumor "; Journal of Biomedical Optics, 12 (1): 014001-1-8,2007.Owing to adopt femtosecond pulse laser, system cost is high; Imaging object places water tank, and system flexibility is low.
Summary of the invention
The object of the invention is the deficiency to above-mentioned prior art; A kind of optoacoustic and fluorescent dual module integral computed tomography (SPECT) system and formation method are proposed; Two kinds of image modes are shared LASER Light Source and toy turntable, realize the bimodal imaging at same hardware platform, thereby are lowered into the picture complexity; Reduce hardware cost, improve system flexibility and fluorescence tomography rebuilding precision.
The technical thought that realizes the object of the invention is: utilize the Direct Digital synthetic technology; Produce two kinds of modulation signals; According to imaging demand continuous wave laser is carried out intensity modulated; As optoacoustic computed tomography (SPECT) system and fluorescence computed tomography (SPECT) system excitation source, utilize Air Coupling supersonic sounding array side to receiving photoacoustic signal, obtain the imaging object optical property parameter; Utilize gate ICCD camera to survey the emitting fluorescence signal,, obtain fluorogen position, fluorescent yield and fluorescence lifetime information in the imaging object body in conjunction with the prior information that the optoacoustic fault imaging provides.Utilize dual module integral imaging system involved in the present invention to carry out fault imaging to the cell or the DNA of biological tissue inside labelling.Whole system comprises:
Can modulate LASER Light Source, be used to produce the laser signal of intensity modulated,, be arranged on the galvanometer front end as the imaging irradiation source;
Galvanometer is used to change the point of irradiation of laser on imaging object, to realize multi-point shooting, is arranged between LASER Light Source and the Electrocontrolled sample turntable;
Sample rotary table is used to place imaging object and imaging object is rotated, to accomplish the data of multiple angles collection;
Filter wheel is placed between Electrocontrolled sample turntable and the gate ICCD camera, is used for fluorescence signal is carried out filtering;
Gate ICCD camera is used for multi-angle and gathers biological intravital fluorescence signal, and fluorescence signal is transferred to computer;
The optoacoustic detection acquisition module is used to receive biological intravital photoacoustic signal, and the photoacoustic signal that receives is carried out LPF, low noise amplification and AD sampling, transfers to computer at last;
System control module is used to produce control signal, and divides 5 the tunnel to be transferred to gate ICCD camera, LASER Light Source, galvanometer, sample rotary table and optoacoustic detection acquisition module respectively, receives data storage from the computer index signal that finishes simultaneously;
Computer; Be used to store photoacoustic signal and fluorescence signal; Send after data storage finishes and instruct to system control module; And utilize optoacoustic tomography rebuilding algorithm and fluorescence fault imaging algorithm to handle, obtain imaging object in-vivo tissue light absorption parameter, fluorogen position, fluorescent yield and fluorescence lifetime parameter.
Described modulation signal generator and continuous-wave laser two parts of comprising; The modulation signal generator is used to produce linear FM signal and sine wave signal; Continuous-wave laser is carried out intensity modulated; Constitute excitation source, wherein the linear FM signal of modulation signal generator generation is carried out intensity modulated to continuous-wave laser, constitutes the excitation source of optoacoustic fault imaging; The sine wave signal that the modulation signal generator produces carries out intensity modulated to continuous-wave laser, constitutes the excitation source of fluorescence fault imaging.
Described sample rotary table comprises: motor and sample holder, sample holder are positioned on the motor, and the minimum rotation of motor step-length is 0.5 degree, and sample holder is provided with 6 fixing points, is fixed into extremity, head and the afterbody of picture toy respectively.
Described optoacoustic detection module comprises:
64 yuan of Air Coupling ultrasonic transduction arrays are used to receive photoacoustic signal;
64 channel signal collecting units; The photoacoustic signal that is used for 64 yuan of Air Coupling ultrasonic transductions of parallel acquisition array received; It comprises that bandwidth is the low pass filter of 15MHz, 2 grades of low noise amplifiers and 16bitAD sampler; This low pass filter, low noise amplifier and AD sampler adopt cascade system to link to each other successively, place after the ultrasonic transduction array.
Described system control module comprises:
Field programmable gate array chip and 6 pin connectors.Field programmable gate array chip is used for producing and receiving control signal; This chip links to each other with 6 pin connectors; Wherein 1 pin is used to receive data storage from the computer index signal that finishes, and other 5 pins are used for the control signal that branch 5 tunnel transmission field programmable gate array chips produce, wherein:
The 1 the tunnel transfers to and can modulate LASER Light Source, is used for the switch laser light source and selects the laser modulation signal waveform;
The 2 the tunnel transfers to galvanometer, is used for the switch galvanometer and selects the vibration mirror scanning parameter;
The 3 the tunnel transfers to sample rotary table, is used for the switch motor and selects the rotation step-length;
The 4 the tunnel transfers to gate ICCD camera, is used for the switch of gate ICCD camera shutter;
The 5 the tunnel transfers to the optoacoustic detection acquisition module, is used to control the switch and the acquisition rate of this module.
Described sample rotary table, filter wheel, gate ICCD camera and laser direction are positioned on the straight line, and 64 yuan of Air Coupling ultrasonic transduction Array Method form the vertical sounding mode of decussation to vertical with this straight line.
Utilize said system to carry out the method for optoacoustic and fluorescent dual module integral fault imaging, comprising:
(1) data acquisition step
(1a) can modulate LASER Light Source and produce the synthetic continuous wave laser of linear FM signal; Excitation source as the optoacoustic fault imaging; Produce photoacoustic signal after placing the imaging object absorption luminous energy on the sample rotary table, the optoacoustic detection collecting unit is gathered photoacoustic signal, and transfers to computer;
(1b) can modulate LASER Light Source and produce the synthetic continuous wave laser of sinusoidal signal; Excitation source as the fluorescence fault imaging; Produce fluorescence signal after fluorescent dye or fluorescin are stimulated in the imaging object body; Other signal beyond the narrow band pass filter filtering fluorescence signal on the filter wheel, gate ICCD camera is gathered fluorescence signal, and transfers to computer;
(1c) sample rotary table drives the imaging object rotation, and sample rotary table is gathered after static fully more by the time, repeats until 360 degree finishing collecting;
(1d) change laser and on imaging object, excite the position, carry out the described data acquisition of step (1a)～(1c) again, accomplish until 360 degrees of data collections of a plurality of shot points;
(2) optoacoustic tomography rebuilding step
Computer produces the linear FM signal of digital reference; The photoacoustic signal that step (1) is gathered carries out matched filtering; Filtering output is carried out digital beam and is formed, and carries out Frequency Domain Deconvolution again, carries out quick inversefouriertransform at last; Obtain the light absorption parameter of imaging object in-vivo tissue, obtain the optoacoustic faultage image;
(3) fluorescence tomography rebuilding step
Computer produces the digital reference sinusoidal signal; The fluorescence signal that step (1) is gathered carries out matched filtering; Fourier transformation is carried out in filtering output, and transformation results is adopted alternative manner, with the reconstructed results in the step (2) as the required optical parametric prior information of fluorescence fault imaging; The intravital fluorogen of reestablishment imaging object position, fluorescent yield and fluorescence lifetime information obtain the fluorescence faultage image.
The present invention compared with prior art has following advantage:
First; The present invention uses one can modulate LASER Light Source, through changing modulation waveform, as the radiation source of optoacoustic fault imaging and two kinds of image modes of fluorescence fault imaging; Overcome the deficiency that adopts two cover autonomous devices to carry out two kinds of mode imagings in the prior art; Make the present invention reach light source and share, reduce system bulk, the economize on hardware cost.
The second, the present invention's sample that forms images places on the sample rotary table, adopts the Air Coupling ultrasound transducer array to survey photoacoustic signal; The imaging sample need not to place the cistern device of prior art optoacoustic fault imaging; Make the present invention reduce hardware cost, reduce system complexity, increase and use motility.
The 3rd; Photoacoustic signal of the present invention is surveyed light path and fluorescence signal, and to survey light path vertical each other, carry out two kinds of mode when forming images sample position and attitude remain unchanged, overcome the deficiency that sample position and attitude change in the prior art; Avoid the image registration operation, reduce the data processing complex degree.
The 4th; Fluorescence tomography rebuilding of the present invention is a prior information with optoacoustic tomography rebuilding result; Overcome the deficiency that can not be provided as picture subject inner tissue light absorption parameter in the prior art in real time, improve the reconstruction precision of fluorogen position, fluorescent yield and fluorescence lifetime parameter in the body.
Description of drawings
Fig. 1 is a system structure sketch map of the present invention;
Fig. 2 is an imaging general flow chart of the present invention;
Fig. 3 is optoacoustic tomography rebuilding sub-process figure;
Fig. 4 is fluorescence tomography rebuilding sub-process figure.
The specific embodiment
Below in conjunction with accompanying drawing 1, apparatus system of the present invention done further describing.
Optoacoustic of the present invention and fluorescent dual module integral computed tomography (SPECT) system comprise and can modulate LASER Light Source 1, galvanometer 2, sample rotary table 3, filter wheel 4, gate ICCD camera 5, optoacoustic detection acquisition module 6, system control module 7, computer 8, data/address bus 9 and control bus 10.Wherein:
Can modulate LASER Light Source 1, comprise modulation signal generator and Continuous Wave Tunable laser instrument, this modulation signal generator mainly comprises field programmable gate array chip, Direct Digital synthesis chip and data-interface.The Direct Digital synthesis chip all is connected with field programmable gate array chip with data-interface; The triggering signal that data-interface receives from system control module 7 sends field programmable gate array chip to; Difference according to image mode; Field programmable gate array chip control Direct Digital synthesis chip produces different modulation signals; Comprise linear FM signal and sine wave signal, and be transferred to laser instrument by data-interface and carry out laser intensity modulation, respectively as the excitation source of optoacoustic fault imaging and fluorescence fault imaging.
Sample rotary table 3 comprises motor and sample holder, and motor drives the sample holder rotation, and the step length command signal of motor is from system control module 7, and minimum step is 0.5 degree.Sample rotary table is used to place imaging object, and imaging object is rotated.Imaging object comprises toys such as white mice and transgenic nude mice.When toy is carried out to picture; The physiological movement of toy can impact image quality, and for avoiding the generation of this situation, sample holder is provided with 6 fixing points; Fixing through to toy extremity, head and afterbody at utmost reduces the amplitude of animal physiological motion.
Utilize said system to carry out method for imaging, be described below with reference to Fig. 2:
Step 1, data acquisition
(1a) that the fluorescent probe labelling is good transgenic nude mice is fixed on the sample rotary table, and adjustment transgenic nude mice is to the distance of gate ICCD camera lens and ultrasonic transduction array, makes system can obtain distinct image;
(1b) can modulate LASER Light Source generation linear FM signal modulates as radiation source laser intensity.Transgenic nude mouse inner tissue absorbs luminous energy and produces photoacoustic signal, Air Coupling supersonic sounding array received photoacoustic signal, and signal transfers to high-performance computer through data/address bus after LPF, low noise amplification and AD sampling;
(1c) can modulate LASER Light Source generation sine wave laser intensity is modulated, and irradiation transgenic nude mice.Fluorescent probe absorbs luminous energy emitting fluorescence signal in the transgenic nude mouse; Escape out the excitation signal that also has of animal body surface through the scattering of transgenic nude mice; Escape out the optical signal of animal body surface through the filtering of 40nm bandwidth band pass filter after; Gather by gate ICCD camera, and transfer to high-performance computer through data/address bus;
(1d) in the signal acquisition process, motor is under system control module control, and every rotation 45 photoacoustic signal of degree collection and fluorescence signals repeat said process data acquisition in 360 degree scopes and accomplish;
(1e) after the 360 degrees of data finishing collecting, change laser irradiation at transgenic nude mice point on one's body, carry out data acquisition again,, satisfy optoacoustic and the requirement of fluorescence tomography rebuilding until collecting enough data through vibration mirror scanning.
With reference to Fig. 3, the concrete realization of this step is following:
(2a) computer produces digital reference signal, and this reference signal is identical with the synthetic linear FM signal of laser intensity;
(2b) computer carries out the digital correlation processing to step (2a) digital reference signal that produces and the 64 passage photoacoustic signals that step (1b) collects; And result carried out LPF; For improving operation efficiency, digital correlation and LPF all utilize the frequency domain fast Fourier transform to accomplish;
(2c) computer to 64 passage results of step (2b) delay time add with, obtain digital beam and form the result;
(2d) computer carries out filtered back projection to the wave beam formation result of step (2c), obtains transgenic nude mouse inner tissue light absorption parameter, obtains the optoacoustic faultage image.
With reference to Fig. 4, the concrete realization of this step is following:
(3a) computer produces digital reference signal, and this reference signal is identical with the synthetic sine wave signal of laser intensity;
(3b) computer carries out the digital correlation processing to step (3a) digital reference signal that produces and the fluorescence signal that step (1c) collects; And result carried out LPF; For improving operation efficiency, digital correlation and LPF all utilize the frequency domain fast Fourier transform to accomplish;
(3c) computer carries out fast Fourier transform to the result of step (3b), and computational transformation result's amplitude and phase place;
(3d) result that obtains with step (2d) of computer according to the amplitude and the phase place of step (3c), rebuilds the intravital fluorescent probe of transgenic nude mice position, fluorescent yield and fluorescence lifetime parameter as transgenic nude mice light absorption parameter prior information;
(3e) according to the anti-photon stream density that pushes away nude mice surface fluorescence signal of fluorescent probe position, fluorescent yield and the fluorescence lifetime parameter of step (3d); And with measure fluorescence signal and subtract each other; If the absolute value that subtracts each other the result is greater than threshold value; Like 10-6, then amplitude and phase place are carried out disturbance, and repeating step (3d); If the absolute value that subtracts each other the result like 10-6, then stops iteration less than threshold value, and fluorescent probe position, fluorescent yield and the fluorescence lifetime parameter of inciting somebody to action this moment obtain the fluorescence faultage image as final result.
Claims (8)
1. optoacoustic and fluorescent dual module integral computed tomography (SPECT) system is characterized in that it comprises:
Can modulate LASER Light Source (1), be used to produce the laser signal of intensity modulated,, be arranged on the galvanometer front end as the imaging irradiation source;
Galvanometer (2) is used to change the point of irradiation of laser on imaging object, to realize multi-point shooting, is arranged between LASER Light Source and the Electrocontrolled sample turntable;
Sample rotary table (3) is used to place imaging object and imaging object is rotated, to accomplish the data of multiple angles collection;
Filter wheel (4) is placed between Electrocontrolled sample turntable and the gate ICCD camera, is used for fluorescence signal is carried out filtering;
Gate ICCD camera (5) is used for multi-angle and gathers biological intravital fluorescence signal, and fluorescence signal is transferred to computer;
Optoacoustic detection acquisition module (6) is used to receive biological intravital photoacoustic signal, and the photoacoustic signal that receives is carried out LPF, low noise amplification and AD sampling, transfers to computer at last;
System control module (7) is used to produce control signal, and divides 5 the tunnel to be transferred to gate ICCD camera, LASER Light Source, galvanometer, sample rotary table and optoacoustic detection acquisition module respectively, receives data storage from the computer index signal that finishes simultaneously;
Computer (8); Be used to store photoacoustic signal and fluorescence signal; Send after data storage finishes and instruct to system control module; And utilize optoacoustic tomography rebuilding algorithm and fluorescence fault imaging algorithm to handle, obtain imaging object in-vivo tissue light absorption parameter, fluorogen position, fluorescent yield and fluorescence lifetime parameter.
2. optoacoustic according to claim 1 and fluorescent dual module integral computed tomography (SPECT) system; It is characterized in that: can modulate LASER Light Source (1) and comprise modulation signal generator and continuous-wave laser two parts; The modulation signal generator is used to produce linear FM signal and sine wave signal; Continuous-wave laser is carried out intensity modulated, constitute excitation source.
3. optoacoustic according to claim 2 and fluorescent dual module integral computed tomography (SPECT) system is characterized in that: the linear FM signal that the modulation signal generator produces is carried out intensity modulated to continuous-wave laser, constitutes the excitation source of optoacoustic fault imaging; The sine wave signal that the modulation signal generator produces carries out intensity modulated to continuous-wave laser, constitutes the excitation source of fluorescence fault imaging.
4. optoacoustic according to claim 1 and fluorescent dual module integral computed tomography (SPECT) system; It is characterized in that: sample rotary table (2) comprises motor and sample holder; Sample holder is positioned on the motor; The minimum rotation of motor step-length is 0.5 degree, and sample holder is provided with 6 fixing points, is fixed into extremity, head and the afterbody of picture toy respectively.
5. optoacoustic according to claim 1 and fluorescent dual module integral computed tomography (SPECT) system is characterized in that optoacoustic detection module (6), comprising:
64 yuan of Air Coupling ultrasonic transduction arrays are used to receive photoacoustic signal;
64 channel signal collecting units; The photoacoustic signal that is used for 64 yuan of Air Coupling ultrasonic transductions of parallel acquisition array received; It comprises that bandwidth is the low pass filter of 15MHz, 2 grades of low noise amplifiers and 16bitAD sampler; This low pass filter, low noise amplifier and AD sampler adopt cascade system to link to each other successively, place after the ultrasonic transduction array.
6. optoacoustic according to claim 1 and fluorescent dual module integral computed tomography (SPECT) system is characterized in that system control module (7), comprising:
Field programmable gate array chip and 6 pin connectors.Field programmable gate array chip is used for producing and receiving control signal; This chip links to each other with 6 pin connectors; Wherein 1 pin is used to receive data storage from the computer index signal that finishes, and other 5 pins are used for the control signal that branch 5 tunnel transmission field programmable gate array chips produce, wherein:
The 1 the tunnel transfers to and can modulate LASER Light Source, is used for the switch laser light source and selects the laser modulation signal waveform;
The 2 the tunnel transfers to galvanometer, is used for the switch galvanometer and selects the vibration mirror scanning parameter;
The 3 the tunnel transfers to sample rotary table, is used for the switch motor and selects the rotation step-length;
The 4 the tunnel transfers to gate ICCD camera, is used for the switch of gate ICCD camera shutter;
The 5 the tunnel transfers to the optoacoustic detection acquisition module, is used to control the switch and the acquisition rate of this module.
7. optoacoustic according to claim 1 and fluorescent dual module integral computed tomography (SPECT) system; It is characterized in that: sample rotary table, filter wheel, gate ICCD camera and laser direction are positioned on the straight line; 64 yuan of Air Coupling ultrasonic transduction Array Method form the vertical sounding mode of decussation to vertical with this straight line.
8. optoacoustic and fluorescent dual module integral tomograph imaging method comprise:
(1) data acquisition step
(1a) can modulate LASER Light Source and produce the synthetic continuous wave laser of linear FM signal; Excitation source as the optoacoustic fault imaging; Produce photoacoustic signal after placing the imaging object absorption luminous energy on the sample rotary table, the optoacoustic detection collecting unit is gathered photoacoustic signal, and transfers to computer;
(1b) can modulate LASER Light Source and produce the synthetic continuous wave laser of sinusoidal signal; Excitation source as the fluorescence fault imaging; Produce fluorescence signal after fluorescent dye or fluorescin are stimulated in the imaging object body; Other signal beyond the narrow band pass filter filtering fluorescence signal on the filter wheel, gate ICCD camera is gathered fluorescence signal, and transfers to computer;
(1c) sample rotary table drives the imaging object rotation, and sample rotary table is gathered after static fully more by the time, repeats until 360 degree finishing collecting;
(1d) change laser and on imaging object, excite the position, carry out the described data acquisition of step (1a)～(1c) again, accomplish until 360 degrees of data collections of a plurality of shot points;
(2) optoacoustic tomography rebuilding step
Computer produces the linear FM signal of digital reference; The photoacoustic signal that step (1) is gathered carries out matched filtering; Filtering output is carried out digital beam and is formed, and carries out Frequency Domain Deconvolution again, carries out quick inversefouriertransform at last; Obtain the light absorption parameter of imaging object in-vivo tissue, obtain the optoacoustic faultage image;
(3) fluorescence tomography rebuilding step
Computer produces the digital reference sinusoidal signal; The fluorescence signal that step (1) is gathered carries out matched filtering; Fourier transformation is carried out in filtering output, and transformation results is adopted alternative manner, with the reconstructed results in the step (2) as the required optical parametric prior information of fluorescence fault imaging; The intravital fluorogen of reestablishment imaging object position, fluorescent yield and fluorescence lifetime information obtain the fluorescence faultage image.
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