CN107567498B - 用于大疱性表皮松解症治疗的与col7a1外显子73配对的寡核苷酸 - Google Patents

用于大疱性表皮松解症治疗的与col7a1外显子73配对的寡核苷酸 Download PDF

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CN107567498B
CN107567498B CN201680015142.2A CN201680015142A CN107567498B CN 107567498 B CN107567498 B CN 107567498B CN 201680015142 A CN201680015142 A CN 201680015142A CN 107567498 B CN107567498 B CN 107567498B
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exon
mrna
oligonucleotide
aon1
col7a1
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CN107567498A (zh
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E·M·海斯马
M·波特曼
W·博伊默
V·布林克斯
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Wings Therapeutics Inc
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CN201680015142.2A 2015-03-11 2016-03-11 用于大疱性表皮松解症治疗的与col7a1外显子73配对的寡核苷酸 Expired - Fee Related CN107567498B (zh)

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GB1504124.7 2015-03-11
GBGB1504124.7A GB201504124D0 (en) 2015-03-11 2015-03-11 Oligonucleotides
PCT/EP2016/055360 WO2016142538A1 (en) 2015-03-11 2016-03-11 Oligonucleotides matching col7a1 exon 73 for epidermolysis bullosa therapy

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Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1253335A1 (zh) * 2015-06-01 2019-06-14 Sarepta Therapeutics, Inc. 在vii型胶原蛋白中反义诱导外显子的排除
US12227743B2 (en) 2018-01-25 2025-02-18 Osaka University Pharmaceutical composition for treatment of diseases associated with upregulated periostin expression or periostin splice variant switching
GB201902735D0 (en) 2019-02-28 2019-04-17 Proqr Therapeutics Ii Bv Oligonucleotides for use in the treatment of dystrophic epidermolysis bullosa
WO2021113270A1 (en) 2019-12-02 2021-06-10 Shape Therapeutics Inc. Therapeutic editing
JP2023554180A (ja) * 2020-12-10 2023-12-26 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル ジストロフィー性表皮水疱症を治療するための物質および方法。
JPWO2024080311A1 (enExample) * 2022-10-12 2024-04-18
CN116555349A (zh) * 2023-01-09 2023-08-08 中吉智药(南京)生物技术有限公司 一种腺相关病毒载体及其构建方法与应用
CN118001404B (zh) * 2024-02-06 2025-02-25 四川大学华西医院 抑制C17orf67基因表达试剂在制备治疗获得性大疱表皮松解症药物中的用途

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676529A (zh) * 2006-05-19 2012-09-19 莱顿教学医院 一种反义寡核苷酸
WO2013053819A1 (en) * 2011-10-11 2013-04-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Exon skipping therapy for dystrophic epidermolysis bullosa
CN103501793A (zh) * 2011-02-08 2014-01-08 夏洛特-梅克伦堡医院(商业用名:卡罗来纳保健系统) 反义寡核苷酸

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US7101993B1 (en) 1990-01-11 2006-09-05 Isis Pharmaceuticals, Inc. Oligonucleotides containing 2′-O-modified purines
US5952221A (en) 1996-03-06 1999-09-14 Avigen, Inc. Adeno-associated virus vectors comprising a first and second nucleic acid sequence
WO1997041208A1 (en) 1996-04-26 1997-11-06 Case Western Reserve University Skin regeneration using mesenchymal stem cells
JP2002080662A (ja) 2000-09-08 2002-03-19 Jsr Corp ゴム組成物
EP1191097A1 (en) 2000-09-21 2002-03-27 Leids Universitair Medisch Centrum Induction of exon skipping in eukaryotic cells
EP2221376B1 (en) 2001-06-21 2012-11-21 Isis Pharmaceuticals, Inc. Antisense modulation of superoxide dismutase 1, soluble expression
AU2002366799A1 (en) 2001-12-19 2003-07-09 Alwyn Company, Inc. Allantoin-containing preparations for administration as gels and aerosols
US20040096833A1 (en) 2002-11-16 2004-05-20 Isis Pharmaceuticals Inc. Modulation of FBP-interacting repressor expression
US20040092464A1 (en) 2002-11-11 2004-05-13 Isis Pharmaceuticals Inc. Modulation of mitogen-activated protein kinase kinase kinase 11 expression
DE10238298A1 (de) 2002-08-21 2004-03-04 Beiersdorf Ag Verwendung von Antisense-Oligonucleotiden zur Behandlung von degenerativen Hauterscheinungen
AU2003225410A1 (en) 2003-03-21 2004-10-11 Academisch Ziekenhuis Leiden Modulation of exon recognition in pre-mrna by interfering with the secondary rna structure
US8765169B2 (en) 2004-02-13 2014-07-01 Smith & Nephew, Inc. Wound healing profile
WO2005086768A2 (en) 2004-03-11 2005-09-22 Albert Einstein College Of Medicine Of Yeshiva University Enhanced production of functional proteins from defective genes
HRP20110352T1 (hr) 2004-06-28 2011-06-30 The University Of Western Australia Antisens olinukleotidi za induciranje zaobilaženja egzona i postupci njihove upotrebe
GB0505202D0 (en) 2005-03-14 2005-04-20 Intercytex Ltd Skin equivalent culture
WO2007069813A1 (en) 2005-12-16 2007-06-21 Modern Cell & Tissue Technologies Inc. Methods for culturing mesenchymal stem cell and compositions comprising mesenchymal stem cell for reparing skin defects
PT2161038E (pt) 2006-01-26 2014-03-10 Isis Pharmaceuticals Inc Composições e suas utilizações dirigidas à huntingtina
PL2049664T3 (pl) 2006-08-11 2012-02-29 Biomarin Tech Bv Jednoniciowe oligonukleotydy komplementarne do powtarzalnych elementów do leczenia chorób genetycznych związanych z niestabilnością powtórzeń DNA
EP2134848A2 (en) 2007-03-07 2009-12-23 Aarhus Universitet Pig model for breast cancer, mitochondria related protein folding disorders and/or epidermolysis bullosa simplex
US8077297B2 (en) 2008-06-30 2011-12-13 Nellcor Puritan Bennett Ireland Methods and systems for discriminating bands in scalograms
EP2151248A1 (en) 2008-07-30 2010-02-10 Johann Bauer Improved pre-mRNA trans-splicing molecule (RTM) molecules and their uses
EP2601294B1 (en) 2010-08-05 2018-11-28 Academisch Ziekenhuis Leiden h.o.d.n. LUMC Antisense oligonucleotide directed removal of proteolytic cleavage sites from proteins
US9603949B2 (en) * 2011-09-01 2017-03-28 University Of Iowa Research Foundation Oligonucleotide-based probes for detection of bacterial nucleases
JP6177243B2 (ja) * 2011-10-06 2017-08-09 ミラゲン セラピューティクス， インコーポレイテッド マイクロｒｎａの調節による全身エネルギーホメオスタシスの制御
KR20150009521A (ko) 2012-03-07 2015-01-26 파이브로셀 테크놀로지스, 인코퍼레이티드 국소피부제제 및 개인맞춤형 피부치료방법
WO2013173637A1 (en) 2012-05-16 2013-11-21 Rana Therapeutics, Inc. Compositions and methods for modulating gene expression
EP3702461A1 (en) 2013-07-08 2020-09-02 Institut National De La Sante Et De La Recherche Medicale - Inserm Methods for performing antisense oligonucleotide-mediated exon skipping in the retina of a subject in need thereof
PT3220924T (pt) 2014-11-17 2022-01-12 Rev Pharma Corp Medicamento tópico para lesões da pele e mucosas associadas à epidermólise bolhosa
HK1253335A1 (zh) 2015-06-01 2019-06-14 Sarepta Therapeutics, Inc. 在vii型胶原蛋白中反义诱导外显子的排除
SI3307277T1 (sl) 2015-06-15 2021-02-26 Tirmed Pharma Ab Enoverižni oligonukleotidi za uporabo pri zdravljenju motenj kože
WO2017078526A2 (en) 2015-11-05 2017-05-11 Rijksuniversiteit Groningen Antisense oligonucleotide-mediated exon skipping as systemic therapy for recessive dystrophic epidermolysis bullosa (rdeb).

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676529A (zh) * 2006-05-19 2012-09-19 莱顿教学医院 一种反义寡核苷酸
CN103501793A (zh) * 2011-02-08 2014-01-08 夏洛特-梅克伦堡医院(商业用名:卡罗来纳保健系统) 反义寡核苷酸
WO2013053819A1 (en) * 2011-10-11 2013-04-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Exon skipping therapy for dystrophic epidermolysis bullosa

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Mutation analysis and characterization of COL7A1 mutations in dystrophic epidermolysis bullosa;Ningning Dang等;《Experimental Dermatology》;20080701;第17卷(第7期);第553-568页 *

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US20180216106A1 (en) 2018-08-02
IL254173B (en) 2022-03-01
WO2016142538A1 (en) 2016-09-15
HUE050898T2 (hu) 2021-01-28
JP6929783B2 (ja) 2021-09-01
EA035732B1 (ru) 2020-07-31
NZ736083A (en) 2021-08-27
ES2803651T3 (es) 2021-01-28
JP2022000020A (ja) 2022-01-04
US11352626B2 (en) 2022-06-07
JP2018509903A (ja) 2018-04-12
MX384959B (es) 2025-03-14
EP3268474A1 (en) 2018-01-17
IL254173A0 (en) 2017-10-31
PL3268474T3 (pl) 2020-08-24
AU2016231067B2 (en) 2022-04-21
JP7188810B2 (ja) 2022-12-13
CN107567498A (zh) 2018-01-09
AU2016231067A1 (en) 2017-10-26
MX2021009135A (es) 2021-09-10
SI3268474T1 (sl) 2020-07-31
EP3763816A1 (en) 2021-01-13
KR20170120617A (ko) 2017-10-31
HRP20200944T1 (hr) 2020-09-18
DK3268474T3 (da) 2020-06-15
PT3268474T (pt) 2020-06-17
MX2017011599A (es) 2018-06-15
US20200399638A1 (en) 2020-12-24
EA201791986A1 (ru) 2018-01-31
GB201504124D0 (en) 2015-04-22
BR112017019225A2 (pt) 2018-05-08
EP3268474B1 (en) 2020-05-27
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