CN107541470A - A kind of culture medium for bringing back to life pleurotus eryngii quel strains and its preparation method and application - Google Patents
A kind of culture medium for bringing back to life pleurotus eryngii quel strains and its preparation method and application Download PDFInfo
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- CN107541470A CN107541470A CN201711001075.1A CN201711001075A CN107541470A CN 107541470 A CN107541470 A CN 107541470A CN 201711001075 A CN201711001075 A CN 201711001075A CN 107541470 A CN107541470 A CN 107541470A
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- pleurotus eryngii
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Abstract
A kind of culture medium for bringing back to life pleurotus eryngii quel strains and its preparation method and application, is related to edible mushroom culture technique field.A kind of culture medium for bringing back to life pleurotus eryngii quel strains provided by the invention, counts, is mainly made up of following raw material in parts by weight：20~40 parts of carrot, 1~5 part of glucose, 1~5 part of agar.The culture medium is brought back to life to pleurotus eryngii quel strains, effect of rejuvenation is good, and culture raw material sources are wide, are easy to get, and cost is cheap.A kind of preparation method for the culture medium for bringing back to life pleurotus eryngii quel strains, including：Raw material is mixed with water.Preparation technology is simple, easy to operate.
Description
Technical field
The present invention relates to edible mushroom culture technique field, and more particularly to it is a kind of bring back to life pleurotus eryngii quel strains culture medium and its
Preparation method and application.
Background technology
Pleurotus eryngii also known as pleurotus eryngii, cap is plump, and quality is tender and crisp, because it has the fragrance of almond and the mouth such as abalone
Feel and gain the name.Pleurotus eryngii has reducing blood lipid, norcholesterol, promotes gastro-intestinal digestion, strengthen body immunity, prevent cardiovascular disease
And other effects, pole is liked by people.It is the Rare edible fungus kind that collection is edible, medicine is used for one.
Strain refers to the propagating materials of edible mushroom mycelium and its growth substrate composition.Culture presevation is with dry, low
The means of gentle isolation air, reduce the rate of metabolism of microorganism fungus kind, the vital movement of strain is in semipermanent
Resting state.Atoleine method is usually 2~3 years for the storage life of edible fungus species, past more than the strain of storage life
Toward being difficult to bring back to life by conventional medium, or resuscitable strain, its growth rate is slow, and vigor is low, it is impossible to meet production and
Scientific research requirements, the loss and waste of microorganism resource are caused, great loss is brought to production and scientific research breeding work.
The content of the invention
It is an object of the invention to provide a kind of culture medium for bringing back to life pleurotus eryngii quel strains, the culture medium is answered pleurotus eryngii quel strains
Work, effect of rejuvenation are good, and culture raw material sources are wide, are easy to get, and cost is cheap.
Another object of the present invention is to provide a kind of preparation method for the culture medium for bringing back to life pleurotus eryngii quel strains, preparation technology
Simply, it is easy to operate.
A further object of the present invention is the application for providing the culture medium of above-mentioned resurrection pleurotus eryngii quel strains.
The present invention is solved its technical problem and realized using following technical scheme：
The present invention proposes a kind of culture medium for bringing back to life pleurotus eryngii quel strains, counts, is mainly made up of following raw material in parts by weight：
20~40 parts of carrot, 1~5 part of glucose, 1~5 part of agar.
The present invention proposes a kind of preparation method for the culture medium for bringing back to life pleurotus eryngii quel strains, including：Raw material is mixed with water.
The present invention proposes application of the culture medium of above-mentioned resurrection pleurotus eryngii quel strains in edible mushroom is brought back to life.
A kind of beneficial effect of the culture medium of resurrection pleurotus eryngii quel strains of the embodiment of the present invention and its preparation method and application
It is：
A kind of culture medium for bringing back to life pleurotus eryngii quel strains, using carrot, glucose and agar as raw material, carrot contains albumen
Matter, carbohydrate, fat, carrotene, vitamin A, vitamin B1, vitamin B2, anthocyanidin, calcium, iron, sodium, potassium etc. are a variety of
Nutritional ingredient, the necessary material brought back to life is provided for pleurotus eryngii quel strains, improves the resurrection rate of pleurotus eryngii quel strains.Simultaneously, there is provided apricot Bao
Nutrition needed for mushroom strains growth, breeding.Glucose provides energy to strain, and auxiliary promotes the resurrection of strain, there is provided pleurotus eryngii
Energy necessary to strain grows after bringing back to life.Agar is the polysaccharide body extracted in marine alga.After water extraction, nutrition is more easy to be inhaled carrot
Receive.Obtained culture medium is brought back to life to pleurotus eryngii quel strains, effect of rejuvenation is good, and culture raw material sources are wide, are easy to get, and cost is cheap.It is a kind of
The preparation method of the culture medium of pleurotus eryngii quel strains is brought back to life, preparation technology is simple, easy to operate.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional production that can be obtained by commercially available purchase
Product.
Culture medium of a kind of resurrection pleurotus eryngii quel strains of the embodiment of the present invention and its preparation method and application is carried out below
Illustrate.
A kind of culture medium for bringing back to life pleurotus eryngii quel strains provided in an embodiment of the present invention, is counted, mainly by following in parts by weight
Raw material is made：20~40 parts of carrot, 1~5 part of glucose, 1~5 part of agar.Such as can take carrot 21,22,24,36,
36.5th, 39 parts, glucose 1.2,1.8,3.5,4.3 parts, agar 3.7,4.2,4.5 parts.
Further, in present pre-ferred embodiments, count in parts by weight, raw material includes：25~35 parts of carrot, Portugal
1~3 part of grape sugar, 1~3 part of agar.Such as can take carrot 27,29,31,34 parts, glucose 1.1,1.4,2.4 parts, agar
1.5th, 2.6 parts.
Further, in present pre-ferred embodiments, count in parts by weight, raw material includes：30 parts of carrot, glucose 2
Part, 2 parts of agar.
Carrot is rich in carbohydrate, protein, carbohydrate, fat, carrotene, vitamin A, vitamin B1, vitamin
The nutritional ingredients such as B2, anthocyanidin, calcium, iron, sodium, potassium.In every 100 grams of carrots, containing about 0.6 gram of protein, 0.3 gram of fat, carbohydrate
7.6~8.3 grams, 0.6 milligram of iron, 1.35~17.25 milligrams of provitamin A (carrotene), vitaminB10 .02~0.04 is in the least
Gram, 0.04~0.05 milligram of vitamin B2,12 milligrams of vitamin C, the kilojoule of heat 150.7, separately containing pectin, starch, inorganic salts and
Several amino acids.
In the present invention, the raw material using carrot as culture medium, the nutriment enriched in carrot is to pleurotus eryngii quel strains
The necessary material brought back to life is provided, improves the resurrection rate of pleurotus eryngii quel strains.Meanwhile the nutriment in carrot causes pleurotus eryngii bacterium
Fast-growth, breeding after kind is brought back to life.
Glucose is a kind of important monose, is a kind of polyhydroxy aldehyde.Glucose is the energy source of living cells and new old
Metabolic intermediate, it is the main Energy supply material of biology.Glucose easily absorbs as carbon source, strain, promotes the growth of strain.
During pleurotus eryngii quel strains are brought back to life, glucose auxiliary carrot promotes the resurrection of strain, and provides pleurotus eryngii quel strains resurrection
Energy necessary to grow afterwards.
Agar is one kind of natural plant gum, is the polysaccharide body extracted in marine alga.In the present invention, the solidification as culture medium
Agent.
A kind of preparation method of culture medium for bringing back to life pleurotus eryngii quel strains provided in an embodiment of the present invention, including：
Mass ratio according to carrot, glucose, agar and water is 15~20:1:1:40~50.Weigh raw material.
Carrot is cleaned, dried, is cut into small pieces, preferably, carrot is smashed.Carrot block or carrot disintegrating slag are carried out
Extraction.The nutriment in carrot is extracted by extraction so that pleurotus eryngii quel strains are more easy to absorb.Of the invention real
Apply in example, extracted by the way of boiling.Specifically, carrot block or carrot disintegrating slag are boiled into 15~25min in boiling water, filter
Take filtrate.Carrot filtrate is mixed with glucose, agar and water.Obtained Medium's PH Value is neutrality.
In order to ensure the pure of culture medium, the resurrection of the microbiological effect pleurotus eryngii quel strains such as bacterium, fungi is prevented, will be mixed
Thing is 115~125 DEG C in temperature, and under conditions of pressure is 0.07~0.15MPa, sterilize 15~25min.
The culture medium provided by the invention for bringing back to life pleurotus eryngii quel strains has preferable On Reactivation, and it is edible to can apply to resurrection
Bacterium.Edible mushroom includes pleurotus eryngii quel strains, it is preferable that pleurotus eryngii quel strains are the strain of preservation 10 years.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of culture medium for bringing back to life pleurotus eryngii quel strains is present embodiments provided, raw material includes：Carrot 200g, glucose
10g, agar 10g, water.
Main be prepared by the following method forms：
Carrot is cut into block, 20min is boiled in boiling water, filters to take filtrate.
Filtrate is mixed with glucose, agar, adds water to be settled to 1L.
By mixture under conditions of 115 DEG C, 0.1MPa, sterilize 15min, obtains culture medium.
Embodiment 2
A kind of culture medium for bringing back to life pleurotus eryngii quel strains is present embodiments provided, raw material includes：Carrot 400g, glucose
10g, agar 20g, water.
Main be prepared by the following method forms：
Carrot is cut into block, 15min is boiled in boiling water, filters to take filtrate.
Filtrate is mixed with glucose, agar, adds water to be settled to 1L.
By mixture under conditions of 125 DEG C, 0.15MPa, sterilize 25min, obtains culture medium.
Embodiment 3
A kind of culture medium for bringing back to life pleurotus eryngii quel strains is present embodiments provided, raw material includes：Carrot 250g, glucose
15g, agar 15g, water.
Main be prepared by the following method forms：
Carrot is cut into block, 20min is boiled in boiling water, filters to take filtrate.
Filtrate is mixed with glucose, agar, adds water to be settled to 1L.
By mixture under conditions of 121 DEG C, 0.11MPa, sterilize 20min, obtains culture medium.
Embodiment 4
A kind of culture medium for bringing back to life pleurotus eryngii quel strains is present embodiments provided, raw material includes：Carrot 250g, glucose
10g, agar 20g, water.
Main be prepared by the following method forms：
Carrot is cut into block, 15min is boiled in boiling water, filters to take filtrate.
Filtrate is mixed with glucose, agar, adds water to be settled to 1L.
By mixture under conditions of 118 DEG C, 0.09MPa, sterilize 15min, obtains culture medium.
Embodiment 5
A kind of culture medium for bringing back to life pleurotus eryngii quel strains is present embodiments provided, raw material includes：Carrot 400g, glucose
20g, agar 10g, water.
Main be prepared by the following method forms：
Carrot is cut into block, 20min is boiled in boiling water, filters to take filtrate.
Filtrate is mixed with glucose, agar, adds water to be settled to 1L.
By mixture under conditions of 120 DEG C, 0.11MPa, sterilize 20min, obtains culture medium.
Embodiment 6
A kind of culture medium for bringing back to life pleurotus eryngii quel strains is present embodiments provided, raw material includes：Carrot 300g, glucose
20g, agar 20g, water.
Main be prepared by the following method forms：
Carrot is cut into block, 20min is boiled in boiling water, filters to take filtrate.
Filtrate is mixed with glucose, agar, adds water to be settled to 1L.
By mixture under conditions of 121 DEG C, 0.11MPa, sterilize 20min, obtains culture medium.
Comparative example 1
This comparative example provides PDA culture medium, i.e. potato dextrose agar.
Comparative example 2
This comparative example provides CPDA culture mediums, i.e. potato dextrose agar complete medium.
Test example
The strain block that 8 block sizes about 0.5cm × 0.5cm preserves in atoleine is taken with inoculation shovel, strain block is being tried
Gently it is switched to embodiment 1~6, comparative example 1, comparative example 2 by reducing the vasoliniment of absorption under several on tube wall and provides
In test tube slant culture medium, silica gel plug is stoppered, the lucifuge culture under conditions of 25 DEG C, observes and records growing state, it is as a result as follows
Table：
The pleurotus eryngii parent species sprouting situation of table 1
| Sprout time | Hypha form | |
| Embodiment 1 | About 2 days | It is pure white sparse |
| Embodiment 2 | About 2 days | It is pure white sparse |
| Embodiment 3 | About 1 day | It is pure white dense |
| Embodiment 4 | About 1 day | It is pure white dense, it is energetic |
| Embodiment 5 | About 1 day | It is pure white dense, it is energetic |
| Embodiment 6 | About 1 day | It is pure white dense, it is energetic |
| Comparative example 1 | About 3 days | Hyphae length is few, tiny |
| Comparative example 2 | About 3 days | Hyphae length is few, tiny |
As shown in Table 1, compared to comparative example 1 and comparative example 2, the culture medium for the resurrection pleurotus eryngii quel strains that embodiment 1~6 provides
There is preferable On Reactivation to pleurotus eryngii parent species, sprout time is shorter.Wherein, the resurrection pleurotus eryngii that embodiment 4~6 provides
More preferably, the parent species mycelia of resurrection is pure white dense, energetic for the On Reactivation of the culture medium of strain.Embodiment 6 brings back to life the mother of culture
Kind is compared with the parent species that comparative example 1 and comparative example 2 are cultivated, and the parent species sprout time that embodiment 6 is brought back to life shortens 160%, and growth is fast
Rate improves 72%, illustrates that resurrection ability of the culture medium of the offer of embodiment 6 to pleurotus eryngii quel strains is significantly better than conventional PDA cultures
Base and CPDA culture mediums.Therefore, the formula of the culture medium for the resurrection pleurotus eryngii quel strains that embodiment 6 provides and preparation method more section
It is reasonable to learn.
In summary, the culture medium provided in an embodiment of the present invention for bringing back to life pleurotus eryngii quel strains, with carrot, glucose and fine jade
Fat as raw material, carrot contain protein, carbohydrate, fat, carrotene, vitamin A, vitamin B1, vitamin B2,
The multiple nutritional components such as anthocyanidin, calcium, iron, sodium, potassium, to provide the necessary material brought back to life to pleurotus eryngii quel strains, improve pleurotus eryngii
The resurrection rate of strain.Simultaneously, there is provided the nutrition needed for pleurotus eryngii quel strains growth, breeding.Glucose is auxiliary to provide energy to strain
Carrot is helped to promote the resurrection of strain, there is provided energy necessary to pleurotus eryngii quel strains grow after bringing back to life.Agar be extracted in marine alga it is more
Sugared body, it is culture medium curing agent.After water extraction, nutrition is more easy to be absorbed carrot.Obtained culture medium is answered pleurotus eryngii quel strains
Work, effect of rejuvenation are good, and culture raw material sources are wide, are easy to get, and cost is cheap.
Embodiments described above is the part of the embodiment of the present invention, rather than whole embodiments.The present invention's
The detailed description of embodiment is not intended to limit the scope of claimed invention, but is merely representative of the selected reality of the present invention
Apply example.Based on the embodiment in the present invention, those of ordinary skill in the art are obtained under the premise of creative work is not made
Every other embodiment, belong to the scope of protection of the invention.
Claims (10)
1. a kind of culture medium for bringing back to life pleurotus eryngii quel strains, it is characterised in that count, be mainly made up of following raw material in parts by weight：
20~40 parts of carrot, 1~5 part of glucose, 1~5 part of agar.
2. the culture medium according to claim 1 for bringing back to life pleurotus eryngii quel strains, it is characterised in that count in parts by weight, it is described
Raw material includes：25~35 parts of the carrot, 1~3 part of the glucose, 1~3 part of the agar.
3. the culture medium according to claim 2 for bringing back to life pleurotus eryngii quel strains, it is characterised in that count in parts by weight, it is described
Raw material includes：30 parts of the carrot, 2 parts of the glucose, 2 parts of the agar.
4. a kind of preparation method for the culture medium for bringing back to life pleurotus eryngii quel strains as claimed any one in claims 1 to 3, its feature
It is, including：The raw material is mixed with water.
5. the preparation method of the culture medium according to claim 4 for bringing back to life pleurotus eryngii quel strains, it is characterised in that described in mixing
Before raw material, the carrot boiling, filtering mix carrot filtrate with the glucose, the agar and the water.
6. the preparation method of the culture medium according to claim 5 for bringing back to life pleurotus eryngii quel strains, it is characterised in that the Hu trailing plants
The mass ratio of fore-telling, the glucose, the agar and the water is 15~20:1:1:40~50.
7. the preparation method of the culture medium according to claim 6 for bringing back to life pleurotus eryngii quel strains, it is characterised in that the raw material
After being mixed with the water, in addition to mixture sterilized.
8. the preparation method of the culture medium according to claim 7 for bringing back to life pleurotus eryngii quel strains, it is characterised in that the side of sterilizing
Method includes：It it is 115~125 DEG C in temperature, under conditions of pressure is 0.07~0.15MPa, sterilize 15~25min.
9. application of the culture medium of the resurrection pleurotus eryngii quel strains as described in any one of claims 1 to 3 in edible mushroom is brought back to life.
10. application of the culture medium according to claim 9 for bringing back to life pleurotus eryngii quel strains in edible mushroom is brought back to life, its feature exist
In the edible mushroom includes preserving the pleurotus eryngii quel strains of at least 10 years.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108419606A (en) * | 2018-01-30 | 2018-08-21 | 山东惠民齐发果蔬有限责任公司 | The application of a kind of preparation method and carrot culture medium of carrot culture medium in edible mushroom Mother culture |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505797A (en) * | 2016-01-11 | 2016-04-20 | 黄山学院 | Culture medium for pleurotus eryngii strain cultivation |
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2017
- 2017-10-24 CN CN201711001075.1A patent/CN107541470A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505797A (en) * | 2016-01-11 | 2016-04-20 | 黄山学院 | Culture medium for pleurotus eryngii strain cultivation |
Non-Patent Citations (2)
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| 方白玉 等: "杏鲍菇母种培养基的优化初探", 《食用菌》 * |
| 谢春芹 等: "杏鲍菇母种最适固化培养基的筛选", 《江苏农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108419606A (en) * | 2018-01-30 | 2018-08-21 | 山东惠民齐发果蔬有限责任公司 | The application of a kind of preparation method and carrot culture medium of carrot culture medium in edible mushroom Mother culture |
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