CN108419606A - The application of a kind of preparation method and carrot culture medium of carrot culture medium in edible mushroom Mother culture - Google Patents
The application of a kind of preparation method and carrot culture medium of carrot culture medium in edible mushroom Mother culture Download PDFInfo
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- CN108419606A CN108419606A CN201810091769.7A CN201810091769A CN108419606A CN 108419606 A CN108419606 A CN 108419606A CN 201810091769 A CN201810091769 A CN 201810091769A CN 108419606 A CN108419606 A CN 108419606A
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- carrot
- culture medium
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- edible mushroom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The present invention provides a kind of preparation methods of carrot culture medium, include the following steps：（1）By carrot cleaning and segmentation；（2）Sterilizing：Blotting paper is placed in the bottom of glassware, then the carrot after segmentation is fitted into glassware, seals, carries out autoclave sterilization；（3）Carrot culture medium：After glassware equipped with carrot after sterilizing is taken out, be cooled to room temperature to get；Carrot culture medium is applied to edible mushroom Mother culture, obtains the excellent parent species of edible mushroom.The preparation method of the carrot culture medium has the advantages that simple, practicability is high, nutrition suitable for promoting and can be fully retained in carrot；The carrot culture medium obtained by the preparation method of the carrot culture medium have the advantages that component it is simple, it is cheap, be easily obtained and quality is uniform；The feature that the carrot culture medium is applied to the edible mushroom parent species of the Mother culture acquisition of various edible mushrooms meets the feature of the excellent parent species of corresponding edible mushroom.
Description
Technical field
The present invention relates to edible mushroom parent species culture technique field, the specially a kind of preparation method and Hu of carrot culture medium
Application of the radish culture medium in edible mushroom Mother culture.
Background technology
Edible mushroom is the macro fungi eaten for the mankind, not only delicious flavour, but also full of nutrition, and such as having reduces blood
Cholesterol in liquid provides the effects that amino acid needed by human, treatment hypertension, therefore, with carrying for people's health consciousness
Height, edible mushroom attract attention as healthy food.The strain of edible mushroom is divided into parent species, original seed and cultigen, wherein parent species are straight
Connect the strain that purebred culture acquisition is carried out using the mycelium of fruit body of edible fungi or edible mushroom.
In the prior art, Mother culture generally use potato agar culture medium, and potato agar culture medium has system
Make that technique is cumbersome, culture medium is more with component and culture medium need to be obtained after infusion, and during infusion, it be easy to cause battalion
Loss is supported, differs so as to cause the bacterial strain quality of Mother culture output, the high-quality edible mushroom bacterial strain of quality always cannot be provided.
Invention content
In consideration of it, the present invention provides a kind of preparation methods of carrot culture medium and carrot culture medium in edible mushroom parent species
The preparation method of application in culture, the carrot culture medium has simple, practicability height, suitable for promoting and can be fully retained
The advantages of nutrition in carrot；Simple with component by the carrot culture medium of the preparation method acquisition of the carrot culture medium,
It is cheap, be easily obtained and advantage that quality is uniform；The Mother culture that the carrot culture medium is applied to various edible mushrooms obtains
The feature of the edible mushroom parent species obtained meets the feature of the excellent parent species of corresponding edible mushroom.Technical scheme is as follows：
A kind of preparation method of carrot culture medium, steps are as follows：
（1）Cleaning and segmentation：Carrot is rinsed well, the fibrous root on surface is removed, surface moisture is drained, is then split, it is standby
With；
（2）Sterilizing：Blotting paper is placed in the bottom of glassware, then by step（1）Carrot after segmentation is packed into glassware
In, sealing carries out autoclave sterilization, spare；In edible mushroom parent species incubation, when the moisture content in culture medium is excessive,
Strain can lead to mycelia anoxic due to slow-growing, or even death of rotting because humidity is excessive, therefore, be put in the bottom of glass culture dish
Blotting paper is set, for absorbing the moisture of the carrot output in sterilization process, excellent growth ring is provided for edible mushroom Mother culture
Border；
（3）Carrot culture medium：By step（2）After the glassware equipped with carrot after middle sterilizing takes out, it is cooled to room temperature, obtains
Obtain carrot culture medium；It it is 25 ~ 28 DEG C since growing environment temperature in edible mushroom parent species incubation, need to be maintained, therefore, directly
When carrying out connecing bacterium to edible fungus species, adaptation time of the strain to growing environment is reduced, the fast-growth of edible mushroom parent species is conducive to.
Preferably, the carrot culture medium preparation method the step of（1）In, the carrot after segmentation is carrot stick or carrot
Block.
Preferably, the carrot culture medium preparation method the step of（2）In, glassware is test tube or culture dish.
Preferably, the carrot culture medium preparation method the step of（2）In, sterilization pressure is 0.13 ~ 0.15Mpa, sterilizing temperature
Degree is 120 ~ 140 DEG C, and sterilization time is 30 ~ 50min.
Preferably, the carrot culture medium preparation method the step of（3）In, cooling temperature is 25 ~ 28 DEG C.
Carrot culture medium the answering in edible mushroom Mother culture obtained according to the preparation method of above-mentioned carrot culture medium
With steps are as follows：
（1）Fruit body of edible fungi strain sterilizes：With a concentration of 75%（v/v）Alcoholic solution the surface of fruit body of edible fungi is wiped
It wipes, 25 ~ 35min is then placed in ultraviolet light and ozone environment, take out, stripping and slicing is spare；
（2）Mother culture：By edible mushroom mycelium or step（1）In fruit body of edible fungi access carrot culture medium after sterilizing,
Then constant temperature incubation under the conditions of 25 DEG C obtains edible mushroom parent species.
Preferably, the step of carrot culture medium is applied in edible mushroom Mother culture（1）In, fruit body of edible fungi strain
Standing time is 30min in ultraviolet light and ozone environment.
The carrot used in the present invention is crisp delicious, the full of nutrition homely vegetables of a germplasm, contains abundant albumen
The nutritional ingredients such as matter, carbohydrate, fat, volatile oil, carrotene, vitamin A, vitamin B1, vitamin B2, anthocyanidin, calcium, iron,
Have the advantages that be easily obtained and manufacturing cost is low.
Compared with prior art, beneficial effects of the present invention：
1, the preparation process of carrot culture medium has the battalion of preparation method simply, suitable for promoting and can be fully retained in carrot
The advantages of supporting substance.
2, simple with component using the carrot culture medium of the preparation method of carrot culture medium provided by the invention acquisition,
The uniform and full of nutrition advantage of quality.
3, the carrot culture medium for obtaining the preparation method of carrot culture medium provided by the invention is applied to edible mushroom
The feature for the edible mushroom parent species that Mother culture obtains is more in line with food relative to the feature for the edible mushroom parent species that PDA culture medium obtains
With the feature of the excellent parent species of bacterium.
4, in the present invention, integrally it regard carrot as mother culture media for the first time, and without remaining any component, the present invention is made to carry
The mother culture media of confession be not only easy to obtain, it is cheap, and have the advantages that ecology, environmental protection, be suitable for extensively using popularization.
Specific implementation mode
The following describes the present invention in detail with reference to examples, the advantages and features of the present invention will be with description and more
It is clear.But examples are merely exemplary, and it is not intended to limit the scope of the present invention in any way.Those skilled in the art should
Understand, without departing from the spirit and scope of the invention, technical scheme of the present invention details can be modified or be replaced
It changes, but these modifications and replacement each fall within protection scope of the present invention.
In the present invention, the size of the carrot stick used for：The size of 0.5cm × 0.4cm × 6cm, carrot block is：
1.5cm×1.5cm×1.5cm；In the present invention, Mother culture is carried out using straw mushroom.
Embodiment 1
A kind of preparation method of carrot culture medium, steps are as follows：
（1）Cleaning and segmentation：Carrot is rinsed well, the fibrous root on surface is removed, surface moisture is drained, is then split, obtain
Carrot stick is obtained, it is spare；
（2）Sterilizing：Blotting paper is placed in the bottom of teat glass, the volume of blotting paper accounts for the 1/4 of test tube total volume, then will step
Suddenly（1）The carrot stick of acquisition is fitted into teat glass, and the volume of carrot stick accounts for the 2/4 of test tube total volume, and sealing carries out high temperature
High pressure sterilization, it is spare；Sterilization pressure is 0.14Mpa, and sterilising temp is 130 DEG C, sterilization time 40min；
（3）Carrot culture medium：By step（2）After the teat glass equipped with carrot after middle sterilizing takes out, 26 DEG C are cooled to, is obtained
Obtain carrot culture medium.
Application of the carrot culture medium obtained according to the preparation method of above-mentioned carrot culture medium in straw mushroom Mother culture,
Steps are as follows：
（1）Straw mushroom fructification strain sterilizes：With a concentration of 75%（v/v）Alcoholic solution to the surface wipes of straw mushroom fructification, so
30min is placed in ultraviolet light and ozone environment afterwards, is taken out, stripping and slicing is spare；
（2）Mother culture：By step（1）In straw mushroom fructification access carrot culture medium after sterilizing, then under the conditions of 25 DEG C
Constant temperature incubation obtains straw mushroom parent species.
In embodiment 1, the straw mushroom parent species external appearance characteristic of acquisition is：Mycelia white powder, elongated, glossy and climbing are in test tube
On inner wall, the chlamydospore of generation is wine-colored chain agglomerate, meets the feature of the excellent parent species of straw mushroom.
Embodiment 2
A kind of preparation method of carrot culture medium, steps are as follows：
（1）Cleaning and segmentation：Carrot is rinsed well, the fibrous root on surface is removed, surface moisture is drained, is then split, obtain
Carrot block is obtained, it is spare；
（2）Sterilizing：Blotting paper is placed in the bottom of glass culture dish, the volume of blotting paper accounts for the 1/4 of culture dish total volume, so
Afterwards by step（1）The carrot block of acquisition is fitted into culture dish, sealing, progress autoclave sterilization, sterilization pressure 0.13Mpa,
Sterilising temp is 120 DEG C, sterilization time 50min；
（3）Carrot culture medium：By step（2）After the glass culture dish equipped with carrot after middle sterilizing takes out, 25 DEG C are cooled to,
Obtain carrot culture medium.
Application of the carrot culture medium obtained according to the preparation method of above-mentioned carrot culture medium in straw mushroom Mother culture,
Steps are as follows：
（1）Straw mushroom fructification strain sterilizes：With a concentration of 75%（v/v）Alcoholic solution to the surface wipes of straw mushroom fructification, so
35min is placed in ultraviolet light and ozone environment afterwards, is taken out, stripping and slicing is spare；
（2）Mother culture：By step（1）In straw mushroom fructification access carrot culture medium after sterilizing, then under the conditions of 25 DEG C
Constant temperature incubation obtains straw mushroom parent species.
In embodiment 2, the straw mushroom parent species external appearance characteristic of acquisition is：Mycelia white powder, sparse and climbing are in culture dish inner wall
On, the chlamydospore of generation is the chain agglomerate of red, meets the feature of the excellent parent species of straw mushroom.
Embodiment 3
A kind of preparation method of carrot culture medium, steps are as follows：
（1）Cleaning and segmentation：Carrot is rinsed well, the fibrous root on surface is removed, surface moisture is drained, is then split, obtain
Carrot stick is obtained, it is spare；
（2）Sterilizing：Blotting paper is placed in the bottom of teat glass, the volume of blotting paper accounts for the 1/4 of test tube total volume, then will step
Suddenly（1）The carrot stick of acquisition is fitted into teat glass, and the volume of carrot stick accounts for the 2/4 of test tube total volume, and sealing carries out high temperature
High pressure sterilization, sterilization pressure 0.15Mpa, sterilising temp are 140 DEG C, sterilization time 30min；
（3）Carrot culture medium：By step（2）After the teat glass equipped with carrot after middle sterilizing takes out, 28 DEG C are cooled to, is obtained
Obtain carrot culture medium.
Application of the carrot culture medium obtained according to the preparation method of above-mentioned carrot culture medium in straw mushroom Mother culture,
Steps are as follows：
Mother culture：Mycelia of Straw Mushroom, Volvariel volvacea is accessed in carrot culture medium, then constant temperature incubation under the conditions of 25 DEG C, obtains straw mushroom
Parent species.
In embodiment 3, the straw mushroom parent species external appearance characteristic of acquisition is：Mycelia silver gray, glossy and climbing in vitro wall
On, the chlamydospore of generation is wine-colored chain agglomerate, meets the feature of the excellent parent species of straw mushroom.
Comparative example 1
A kind of PDA culture medium includes the component of following portions by weight：Potato 200g, glucose 20g, agar 18g, originally
Water 1000ml.
The preparation method of above-mentioned PDA culture medium, steps are as follows：Potato is cleaned into peeling, it is spare；Potato 200g is taken,
Then stripping and slicing puts into 1000ml boiling water, continues to boil 30min, smash to pieces, spare；By two layers of gauze of the potato after smashing to pieces
Filtering, takes filtrate to be put into pot, and glucose 20g is added, then agar 18g is placed on asbestos gauge, and glass rod is used in combination in small fire heating
It is stirred continuously after agar is completely dissolved, is supplemented moisture to 1000ml, then dispense, after the 20min that sterilizes, take out, after cooling
It stores spare.
Above-mentioned PDA culture medium is applied to the culture of straw mushroom parent species.
In comparative example 1, the straw mushroom parent species external appearance characteristic of acquisition is：Mycelia silver gray, mycelia tubbiness, tarnish and part are climbed
It climbs on wall in vitro, the chlamydospore of generation is the agglomerate of white, has difference with the feature of the excellent parent species of straw mushroom.
The feature for the straw mushroom parent species that method provided by the invention obtains it can be seen from 1 ~ embodiment of above-described embodiment 3 is equal
Meet the feature of the excellent parent species of straw mushroom, for comparative example 1 compared to 1 ~ embodiment of embodiment 3, the feature of the straw mushroom parent species of acquisition is opposite
Poor, therefore, the carrot culture medium that method provided by the invention obtains can be that edible mushroom parent species are trained relative to PDA culture medium
It supports and better nutrition is provided, the feature for the edible mushroom parent species that the features of the edible mushroom parent species of acquisition is obtained relative to comparative example 1 is more
Add the feature for meeting the excellent parent species of edible mushroom.
Claims (7)
1. a kind of preparation method of carrot culture medium, which is characterized in that steps are as follows：
（1）Cleaning and segmentation：Carrot is rinsed well, the fibrous root on surface is removed, surface moisture is drained, is then split, it is standby
With；
（2）Sterilizing：Blotting paper is placed in the bottom of glassware, then by step（1）Carrot after segmentation is packed into glassware
In, sealing carries out autoclave sterilization, spare；
（3）Carrot culture medium：By step（2）After the glassware equipped with carrot after middle sterilizing takes out, it is cooled to room temperature, obtains
Obtain carrot culture medium.
2. the preparation method of carrot culture medium as described in claim 1, which is characterized in that in step（1）In, after segmentation
Carrot is carrot stick or carrot block.
3. the preparation method of carrot culture medium as described in claim 1, which is characterized in that in step（2）In, glassware
For test tube or culture dish.
4. the preparation method of carrot culture medium as described in claim 1, which is characterized in that in step（2）In, high temperature and pressure
When sterilizing, sterilization pressure is 0.13 ~ 0.15Mpa, and sterilising temp is 120 ~ 140 DEG C, and sterilization time is 30 ~ 50min.
5. the preparation method of carrot culture medium as described in claim 1, which is characterized in that in step（3）In, cooling temperature
It is 25 ~ 28 DEG C.
6. the carrot culture medium obtained according to the preparation method of claim 1-5 any one of them carrot culture mediums is edible
Application in bacterium Mother culture, which is characterized in that steps are as follows：
（1）Fruit body of edible fungi strain sterilizes：With a concentration of 75%（v/v）Alcoholic solution the surface of fruit body of edible fungi is wiped
It wipes, 25 ~ 35min is then placed in ultraviolet light and ozone environment, take out, stripping and slicing is spare；
（2）Mother culture：By edible mushroom mycelium or step（1）In fruit body of edible fungi access carrot culture medium after sterilizing,
Then constant temperature incubation under the conditions of 25 DEG C obtains edible mushroom parent species.
7. application of the carrot culture medium as claimed in claim 6 in edible mushroom Mother culture, which is characterized in that in step
（1）In, fruit body of edible fungi strain standing time in ultraviolet light and ozone environment is 30min.
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| CN201810091769.7A CN108419606A (en) | 2018-01-30 | 2018-01-30 | The application of a kind of preparation method and carrot culture medium of carrot culture medium in edible mushroom Mother culture |
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| CN201810091769.7A CN108419606A (en) | 2018-01-30 | 2018-01-30 | The application of a kind of preparation method and carrot culture medium of carrot culture medium in edible mushroom Mother culture |
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Citations (4)
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|---|---|---|---|---|
| JP2009189317A (en) * | 2008-02-15 | 2009-08-27 | Takemoto Oil & Fat Co Ltd | Artificial cultivation medium of pleurotus mushroom |
| CN105733957A (en) * | 2016-03-01 | 2016-07-06 | 鲁东大学 | Method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains |
| CN105906415A (en) * | 2016-04-18 | 2016-08-31 | 鲁东大学 | Method for cultivating antrodia camphorata fruiting bodies by use of applewood and culture medium |
| CN107541470A (en) * | 2017-10-24 | 2018-01-05 | 重庆市农业科学院 | A kind of culture medium for bringing back to life pleurotus eryngii quel strains and its preparation method and application |
-
2018
- 2018-01-30 CN CN201810091769.7A patent/CN108419606A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009189317A (en) * | 2008-02-15 | 2009-08-27 | Takemoto Oil & Fat Co Ltd | Artificial cultivation medium of pleurotus mushroom |
| CN105733957A (en) * | 2016-03-01 | 2016-07-06 | 鲁东大学 | Method for culturing cordyceps militaris with slant solid strains effectively instead of liquid strains |
| CN105906415A (en) * | 2016-04-18 | 2016-08-31 | 鲁东大学 | Method for cultivating antrodia camphorata fruiting bodies by use of applewood and culture medium |
| CN107541470A (en) * | 2017-10-24 | 2018-01-05 | 重庆市农业科学院 | A kind of culture medium for bringing back to life pleurotus eryngii quel strains and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 郭成金等: "保藏食用菌几种母种培养基的研究", 《天津师大学报(自然科学版)》 * |
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Application publication date: 20180821 |