CN107519377B - Antibacterial and anti-inflammatory composition, preparation method thereof and oral health care product - Google Patents
Antibacterial and anti-inflammatory composition, preparation method thereof and oral health care product Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8964—Anemarrhena
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/646—Arachnids, e.g. spiders, scorpions, ticks or mites
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/26—Aristolochiaceae (Birthwort family), e.g. heartleaf
- A61K36/268—Asarum (wild ginger)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/808—Scrophularia (figwort)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/23—Sulfur; Selenium; Tellurium; Compounds thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Abstract
The invention relates to the technical field of oral hygiene and health care, in particular to a bacteriostatic and anti-inflammatory composition, a preparation method thereof and a health care product for regulating an oral microenvironment. The antibacterial and anti-inflammatory composition is prepared from the following raw materials in parts by weight: 3-10 parts of spider freeze-dried powder, 10-20 parts of honeysuckle, 8-12 parts of coptis chinensis, 25-35 parts of gypsum, 10-14 parts of rhizoma anemarrhenae, 4-8 parts of asarum, 10-20 parts of radix rehmanniae, 10-14 parts of cortex moutan, 10-20 parts of peony, 10-14 parts of radix scrophulariae, 8-12 parts of holy basil and 3-7 parts of jasmine. The antibacterial and anti-inflammatory composition can effectively inhibit the growth of bacteria; the oral health care nursing product can play a role in diminishing inflammation, inhibiting bacteria, relieving pain, stopping bleeding, refreshing breath and the like, and has obvious curative effects on relieving uncomfortable symptoms of the oral cavity of people and keeping good micro-ecology of the oral cavity.
Description
Technical Field
The invention relates to the technical field of oral hygiene and health care, in particular to a bacteriostatic and anti-inflammatory composition, a preparation method thereof and an oral health care product.
Background
With the continuous improvement of the living standard quality of people, the rhythm of life of people is faster and faster, work remuneration or relatives and friends gathering becomes a very common thing, and peculiar smell of the oral cavity becomes a trouble in interpersonal communication. People who pay no attention to oral cleaning for a long time, the growth of streptococcus mutans in the oral cavity is easy to grow in residual food contained in the oral cavity, the residual food is easy to ferment into harmful dental plaque acid, and finally dental caries is caused. In persons suffering from oral diseases such as dental caries, gingivitis, periodontitis, oral mucositis, and dental caries and periodontal disease, bacteria, especially anaerobic bacteria, are easily grown in the oral cavity, and the bacteria are decomposed to produce sulfides, giving off putrefactive taste, and causing halitosis.
Gingivitis, bleeding and swelling and pain of gum are typical symptoms of periodontal disease, and according to statistics, ninety-seven percent of people in China have the periodontal disease, which is one of the main reasons for tooth loss of people over thirty-five years old. Gingivitis is a common periodontal disease, which is caused by the long-term irritation of teeth due to the formation of soft tartar and hard tartar from food residues, bacteria, etc. around the teeth, improper tooth brushing habits, vitamin deficiencies, etc., as a result of inattention to the oral hygiene. Improving oral health has become an important and difficult task in the field of oral health in our country. Therefore, the oral hygiene health care product with the anti-inflammatory effect and the function of effectively removing the ozostomia becomes a heat-selling product.
The mouthwash is an oral product for cleaning and health care in daily life of people, and is mainly used for keeping the oral cavity and teeth clean, promoting breath freshening, nursing gingiva, maintaining a good microenvironment of the oral cavity and the like. The types of the mouth wash mainly comprise fluoride-containing mouth wash, anti-dental film mouth wash, anti-allergy mouth wash, Chinese herbal medicine mouth wash and the like. At present, the mouthwash containing Chinese herbal medicine components is particularly favored by consumers. The patent of the mouthwash containing Chinese herbal medicine components is more, for example, the invention of publication/publication number CN104688850A provides a traditional Chinese medicine mouthwash, the raw materials mainly comprise scutellaria baicalensis, honeysuckle, coptis chinensis, fructus forsythiae, xylitol and 95% ethanol, and the proportion of the selected raw materials adopts the following components: honeysuckle flower: coptis chinensis: fructus forsythiae: the xylitol is 1: 1: 0.8: 0.8: 1, extracting the selected four traditional Chinese medicines to obtain extracting solutions of scutellaria baicalensis, honeysuckle, coptis chinensis and fructus forsythiae, sterilizing the extracting solutions, adding xylitol according to a proportion, blending, and finally preparing the mouthwash. Also, for example, the invention with publication/announcement number CN104083662A discloses a mouthwash for eliminating halitosis caused by stomatitis and a preparation method thereof, wherein the mouthwash is prepared from the following main raw materials in parts by weight: 10-15 parts of bamboo salt, 6-12 parts of sweet clover, 15-20 parts of honeysuckle, 4-10 parts of lophatherum gracile, 5-15 parts of liquorice, 2-9 parts of coptis chinensis, 15-25 parts of mint and 5-10 parts of radix rehmanniae. The patent products have the effects of refreshing breath, nursing gum, improving oral environment and the like, but have poor effects on the aspects of bacteriostasis, inflammation diminishing, pain relieving, swelling diminishing, bleeding stopping and the like. Therefore, the development of the compound traditional Chinese medicine oral health care product with the functions of inhibiting bacteria, diminishing inflammation, relieving pain, stopping bleeding, preventing and relieving various oral inflammations has important significance for relieving uncomfortable symptoms of the oral cavity of people, keeping good micro-ecology of the oral cavity, relieving interpersonal communication pressure and the like.
Disclosure of Invention
In view of the above, the invention provides a bacteriostatic and anti-inflammatory composition, a preparation method thereof and an oral health care product. The antibacterial and anti-inflammatory composition can effectively inhibit the growth of bacteria, has the effects of diminishing inflammation, relieving pain and stopping bleeding, and has obvious curative effects of relieving uncomfortable symptoms of human oral cavity and keeping good micro-ecology of the oral cavity.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a bacteriostatic and anti-inflammatory composition which is prepared from the following raw materials in parts by weight:
3-10 parts of spider freeze-dried powder, 10-20 parts of honeysuckle, 8-12 parts of coptis chinensis, 25-35 parts of gypsum, 10-14 parts of rhizoma anemarrhenae, 4-8 parts of asarum, 10-20 parts of radix rehmanniae, 10-14 parts of cortex moutan, 10-20 parts of peony, 10-14 parts of radix scrophulariae, 8-12 parts of holy basil and 3-7 parts of jasmine.
Preferably, the feed additive is prepared from the following raw materials in parts by weight:
7 parts of spider freeze-dried powder, 15 parts of honeysuckle, 10 parts of coptis chinensis, 30 parts of gypsum, 12 parts of rhizoma anemarrhenae, 6 parts of asarum, 15 parts of radix rehmanniae, 12 parts of cortex moutan, 15 parts of peony, 12 parts of radix scrophulariae, 10 parts of holy basil and 5 parts of jasmine.
In one embodiment provided by the invention, the composition is prepared from the following raw materials in parts by weight:
3 parts of spider freeze-dried powder, 10 parts of honeysuckle, 12 parts of coptis chinensis, 25 parts of gypsum, 14 parts of rhizoma anemarrhenae, 4 parts of asarum, 20 parts of radix rehmanniae, 10 parts of cortex moutan, 20 parts of peony, 10 parts of radix scrophulariae, 12 parts of holy basil and 3 parts of jasmine.
In another embodiment provided by the invention, the composition is prepared from the following raw materials in parts by weight:
10 parts of spider freeze-dried powder, 20 parts of honeysuckle, 8 parts of coptis chinensis, 35 parts of gypsum, 10 parts of rhizoma anemarrhenae, 8 parts of asarum, 10 parts of radix rehmanniae, 14 parts of cortex moutan, 10 parts of peony, 14 parts of radix scrophulariae, 8 parts of holy basil and 7 parts of jasmine.
The invention also provides a preparation method of the antibacterial and anti-inflammatory composition, which comprises the steps of mixing raw materials of spider freeze-dried powder, honeysuckle, coptis chinensis, gypsum, rhizoma anemarrhenae, asarum, radix rehmanniae, cortex moutan, peony, radix scrophulariae, holy basil and jasmine, extracting with alcohol, and filtering to obtain the antibacterial and anti-inflammatory composition.
In the embodiment provided by the invention, the alcohol extraction is as follows: soaking in ethanol water solution for 10-20 days.
In one embodiment of the present invention, the alcohol extraction time is 15 or 20 days.
Preferably, the ethanol in the ethanol water solution has a volume percentage of 50-70%.
In one embodiment of the present invention, the ethanol in the ethanol aqueous solution is 55% or 60% by volume.
Preferably, the dosage ratio of the raw materials to the ethanol aqueous solution is (100-200) in g/mL: 1000.
preferably, the dosage ratio of the raw materials to the ethanol water solution is (140-150) in g/mL: 1000.
the invention also provides an oral health care product, which comprises the antibacterial and anti-inflammatory composition and pharmaceutically or dietetically acceptable auxiliary materials.
Preferably, the oral care product is in the form of a mouth freshener, chewing gum, toothpaste, tooth gel, tooth powder, mouthwash or tablet.
In one embodiment of the present invention, the oral care product is formulated as: 7 parts of spider freeze-dried powder, 15 parts of honeysuckle, 10 parts of coptis chinensis, 30 parts of gypsum, 12 parts of rhizoma anemarrhenae, 6 parts of asarum, 15 parts of radix rehmanniae, 12 parts of cortex moutan, 15 parts of peony, 12 parts of radix scrophulariae, 10 parts of holy basil, 5 parts of jasmine and 1000mL of 70% vol ethanol.
The invention provides a bacteriostatic and anti-inflammatory composition, a preparation method thereof and an oral health care product. The antibacterial and anti-inflammatory composition is prepared from the following raw materials in parts by weight: 3-10 parts of spider freeze-dried powder, 10-20 parts of honeysuckle, 8-12 parts of coptis chinensis, 25-35 parts of gypsum, 10-14 parts of rhizoma anemarrhenae, 4-8 parts of asarum, 10-20 parts of radix rehmanniae, 10-14 parts of cortex moutan, 10-20 parts of peony, 10-14 parts of radix scrophulariae, 8-12 parts of holy basil and 3-7 parts of jasmine. The invention has the technical effects that:
1. the antibacterial and anti-inflammatory composition can effectively inhibit the growth of bacteria;
2. the oral health care nursing product can play a role in diminishing inflammation, inhibiting bacteria, relieving pain and stopping bleeding, and has obvious curative effects on relieving uncomfortable symptoms of the oral cavity of people and keeping good micro-ecology of the oral cavity.
3. The antibacterial and anti-inflammatory composition and the oral health care product are simple to prepare and are suitable for industrial production.
Detailed Description
The invention discloses a bacteriostatic and anti-inflammatory composition, a preparation method thereof and an oral health care product, and can be realized by appropriately improving process parameters by referring to the contents. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
In the embodiment of the invention, the preparation method of the spider freeze-dried powder comprises the following steps: 1. placing spider frozen at-80 deg.C for more than 48 hr in stainless steel jar, and mashing; 2. inverting the mashed spider into a pulverizer, and freezing and pulverizing for about 30 seconds; 3. freeze-drying Aranea powder for 48 hr, and vacuum packaging; 4. storing the vacuum-packaged spider freeze-dried powder in a refrigeration house at the temperature of-18 ℃.
In the invention, the effects of the medicinal materials in the formula are as follows:
freeze-dried spider powder: cold in nature, bitter in taste, extinguishing wind, relieving spasm, relieving swelling, removing toxic substance, dispelling pathogenic wind, removing dampness, dredging collaterals, and relieving pain.
Honeysuckle flower: is cold in nature and sweet in taste, and has the effects of clearing away heat and toxic materials, relieving inflammation, tonifying deficiency and treating wind;
coptis chinensis: clearing heat, eliminating dampness, purging pathogenic fire and removing toxic substances;
gypsum: sweet, pungent and cold in nature, with the actions of clearing heat and purging fire, relieving restlessness and quenching thirst. Can be used for treating fever due to exogenous pathogens, hyperpyrexia, polydipsia, cough and asthma due to lung heat, excessive stomach fire, headache, and toothache;
rhizoma anemarrhenae: has effects in clearing away heat, purging pathogenic fire, promoting salivation, and moistening dryness;
asarum: has effects in expelling pathogenic wind, dispelling cold, inducing resuscitation, relieving pain, warming lung, and eliminating phlegm;
radix rehmanniae: has effects in clearing away heat and cooling blood;
cortex moutan: has effects in clearing away heat, cooling blood, promoting blood circulation, dispelling blood stasis, and relieving deficiency heat;
peony: has spasmolytic, analgesic, and meridian dredging effects;
figwort root: antibacterial;
holy basil incense: a fragrance;
jasmine flower: has pungent, sweet, and cool effects, and is effective for clearing away heat and toxic materials, and removing dampness.
The antibacterial and anti-inflammatory composition, the preparation method thereof and the raw materials or auxiliary materials used in the oral health care product can be purchased from the market.
The invention is further illustrated by the following examples:
example 1
The traditional Chinese medicine composition of the embodiment comprises the following raw materials in parts by weight:
7g of spider freeze-dried powder, 15g of honeysuckle, 10g of coptis chinensis, 30g of gypsum, 12g of rhizoma anemarrhenae, 6g of asarum, 15g of radix rehmanniae, 12g of cortex moutan, 15g of peony, 12g of radix scrophulariae, 10g of holy basil and 5g of jasmine.
The preparation method comprises the following steps:
soaking the raw materials in 1000mL of 55% edible ethanol at normal temperature for 15 days, and filtering for later use.
Example 2
The traditional Chinese medicine composition of the embodiment comprises the following raw materials in parts by weight:
3g of spider freeze-dried powder, 10g of honeysuckle, 12g of coptis chinensis, 25g of gypsum, 14g of rhizoma anemarrhenae, 4g of asarum, 20g of radix rehmanniae, 10g of cortex moutan, 20g of peony, 10g of radix scrophulariae, 12g of holy basil and 3g of jasmine.
The preparation method comprises the following steps:
soaking the raw materials in 1000mL of 60% edible ethanol at normal temperature for 20 days, and filtering for later use.
Example 3
The traditional Chinese medicine composition of the embodiment comprises the following raw materials in parts by weight:
10g of spider freeze-dried powder, 20g of honeysuckle, 8g of coptis chinensis, 35g of gypsum, 10g of rhizoma anemarrhenae, 8g of asarum, 10g of radix rehmanniae, 14g of cortex moutan, 10g of peony, 14g of radix scrophulariae, 8g of holy basil and 7g of jasmine.
The preparation method comprises the following steps:
soaking the raw materials in 1000mL of 55% edible ethanol at normal temperature for 15 days, and filtering for later use.
Comparative example 1
The raw materials and the dosage of the traditional Chinese medicine composition in the comparative example are as follows (compared with example 1, the spider freeze-dried powder is lacked):
15g of honeysuckle, 10g of coptis chinensis, 30g of gypsum, 12g of rhizoma anemarrhenae, 6g of asarum, 15g of radix rehmanniae, 12g of cortex moutan, 15g of peony, 12g of figwort, 10g of holy basil and 5g of jasmine.
The preparation method comprises the following steps:
soaking the raw materials in 1000mL of 55% edible ethanol at normal temperature for 15 days, and filtering for later use.
Comparative example 2
The raw materials and the dosage of the traditional Chinese medicine composition in the comparative example are as follows (compared with example 1, honeysuckle and figwort are lacked):
7g of spider freeze-dried powder, 10g of coptis chinensis, 30g of gypsum, 12g of rhizoma anemarrhenae, 6g of asarum, 15g of radix rehmanniae, 12g of cortex moutan, 15g of peony, 10g of holy basil and 5g of jasmine.
The preparation method comprises the following steps:
soaking the raw materials in 1000mL of 55% edible ethanol at normal temperature for 15 days, and filtering for later use.
Comparative example 3
The raw materials and the dosage of the traditional Chinese medicine composition in the comparative example are as follows (compared with example 1, gypsum, peony and holy basil are lacked):
7g of spider freeze-dried powder, 15g of honeysuckle, 10g of coptis chinensis, 12g of rhizoma anemarrhenae, 6g of asarum, 15g of radix rehmanniae, 12g of cortex moutan, 12g of radix scrophulariae and 5g of jasmine.
The preparation method comprises the following steps:
soaking the raw materials in 1000mL of 55% edible ethanol at normal temperature for 15 days, and filtering for later use.
Comparative example 4
The raw materials and the preparation method of the traditional Chinese medicine composition refer to the publication/publication No. CN104083662A in the invention example 5:
raw materials: 12 parts of bamboo salt, 9 parts of sweet clover, 18 parts of honeysuckle, 6 parts of lophatherum gracile, 10 parts of liquorice, 5 parts of coptis chinensis, 20 parts of mint and 8 parts of radix rehmanniae.
The preparation method of the mouthwash for eliminating halitosis with stomatitis comprises the following steps:
(1) sun drying herba Lophatheri, grinding into fine powder, and sieving with 100 mesh sieve to obtain folium Bambusae powder;
(2) cleaning herba Menthae, pulping, soaking in 80 deg.C hot water, and filtering to obtain filtrate;
(3) mixing Melilotus officinalis, flos Lonicerae, flos Caryophylli, Glycyrrhrizae radix and Coptidis rhizoma, and moistening with three-stage sake for 45 min;
(4) adding the mixture obtained in the step (3) into the filtrate obtained in the step (2), boiling with strong fire, then decocting with slow fire for 50 minutes, stopping heating, adding the bamboo leaf powder obtained in the step (1), standing for 35 minutes, and taking the supernatant for later use;
(5) and (4) adding bamboo salt into the supernatant obtained in the step (4), slowly boiling for 40 minutes, and fully stirring to obtain the bamboo salt.
Test example 1 in vitro bacteriostatic test
1. Experimental strains
Bacteria: staphylococcus aureus (ATCC 6538), Lactobacillus albicans (ATCC 10231) suspension.
2. Concentration of inoculated strain
The bacterial strain is inoculated with bacterial liquid with the concentration of 50-200 cfu/mL.
Preparing a strain suspension: and (3) diluting the cultured strains respectively by using sterilized normal saline to prepare 50-200 cf' u/mL of strain suspension.
3. Preparation of antibacterial liquid for experiment
In the experiment, the Chinese herbal medicine extracts of examples 1-3 and comparative examples 1-4 are selected as the antibacterial liquid, and the formulas of the antibacterial liquid are all traditional Chinese medicine extraction stock solutions. Wherein the Chinese herbal medicine extract is calculated by crude drug.
And simultaneously preparing a bacteriostatic agent blank oral health care product sample.
4. Mouthwash sample inoculation
Inoculating the oral health care products with specified quantity into the two test bacteria respectively under aseptic condition, and mixing uniformly. And (3) storing the inoculated sample at 20 ℃, and detecting the colony number in the sample at 5 minutes, 30 minutes, 1 hour and 12 hours respectively to judge the bacteriostatic ability of the sample.
5. Experimental results and discussion
(1) Evaluation criteria
The bacteriostatic effect was evaluated as a percentage of the colony count reduction. The index is calculated as follows:
percent reduction in colony number (%) - (A)0-An)/A0×100％
In the formula: a. the0Is the level of microorganisms in the mouthwash at the time of initial addition of the microorganisms; a. thenFor different detection timesThe content of organisms.
The total number of bacterial colonies is reduced by 90% in 30 minutes, and if the total number of bacteria can be maintained in 12 hours, the bacteriostatic effect of the sample is judged to be good.
(2) Test results
TABLE 1 mouthwash inhibition of Staphylococcus aureus experimental results
Note: the result of 0h is the number of colonies at the time of initial inoculation.
TABLE 2 mouthwash inhibition of Lactobacillus and Candida albicans test results
Note: the result of 0h is the number of colonies at the time of initial inoculation.
The test result shows that according to the sanitation standard of disposable sanitary products (GB15979-2002), the oral health care product has the bacteriostatic rate of more than 90 percent on staphylococcus aureus and candida albicans under the test condition, and has stronger bacteriostatic action.
Test example 2 study of bacteriostatic clinical Effect
1. Clinical design
(1) The participants used the dispensed mouthwash to rinse 3 times a day as required within 4 weeks using a double-blind, self-control study.
(2) Sample selection
Volunteer experimenters were recruited and volunteer preliminary screening work was performed 14 days prior to the experiment and included in subjects according to inclusion criteria. All tooth surfaces were cleaned by ultrasound. After 14 days, volunteers were screened again, and 30 subjects with GI ═ 0 were included as official subjects. And (4) cleaning all tooth surfaces of the formal testee by ultrasonic scaling. Plaque stained, indicating PLI ═ 0. Oral soft tissue and oral health were recorded and the experiment was started the same day.
(3) Experimental procedures and test details
The mouthwash of test example 1 containing the active ingredient of example 1 was selected as the test group, and a 20% vol alcohol solution was set as the control group.
30 bottles of each mouthwash are numbered and divided into A, B groups. 1 kind of mouthwash is randomly taken out of each group of bottles, and each 2 kinds of mouthwash form one part, and the total amount is 30 parts, and the parts are respectively put into 30 cartons. 30 subjects were randomly attached to a cardboard box. The subject took 1 bottle of mouthwash in the corresponding carton according to the number of the subject, began the experiment. During the experiment, all mechanical cleaning measures are stopped, the number of the mouth wash corresponding to PLI is recorded after 4 days of gargling, and the mouth wash is recovered. And (5) checking the residual amount of the mouthwash, judging compliance, and eliminating experiments if the compliance is obviously poor. Then the daily cleaning measures are resumed for 10 days to eliminate possible residual effects and influences of the former mouthwash. After 10 days, the patient was again thoroughly cleansed and stained with plaque, indicating PLI 0, and another bottle of mouthwash was taken from the subject, using the same procedure as the previous one. And (5) carrying out numbering by numbering personnel to reduce the numbering of all the mouthwash into a test group and a control group, and counting the PLI corresponding to each group.
(4) Instruction for Subjects to use mouthwash on their own
The rinse designated when the group of subjects was issued was for their use at home. Their mouth rinses were instructed to be used at 20ml 3 times a day for 30 seconds each time. Diet and smoking were not restricted.
(5) Test site and test standard
The tested part: #11, #16, #26, #31 labial and buccal surfaces, #36, #46 lingual surfaces. And (4) testing standard: the examined tooth surfaces were stained with a 2% basic fuchsin solution and the plaque index (PLI) was recorded using the modified Qiagley-Hein method.
2. Results of plaque inhibition studies
TABLE 3 plaque index test results (Unit: particle)
| Group of | 0 degree | 1 degree | 2 degree | 3 degree | 4 degree | 5 degree |
| Experimental group | 62 | 76 | 25 | 9 | 3 | 5 |
| Control group | 0 | 28 | 22 | 56 | 52 | 22 |
The results of the plaque index measurements in 30 subjects showed: compared with the control group, the experimental group can obviously inhibit the reformation of dental plaque (P < 0.001).
Test example 3 clinical test for treating halitosis
In 2016 from 9 to 7, 2017, 400 patients are screened in 5 hospitals, wherein the patients are 65 and 18 years old at the maximum age and all suffer from halitosis caused by stomatitis, the patients are randomly divided into two groups, namely 200 patients in an experimental group and 200 patients in a control group, the experimental group uses the mouthwash containing the effective components prepared in the experimental example 1, the control group uses sanjiu oral cleaner antibacterial liquid, the observation period is 3 days, the symptoms of the patients are comprehensively checked 1 day before the experiment, and the patients are regularly checked and inquired during the experiment and well recorded.
The using method comprises the following steps: in order to achieve the best treatment effect, the gargle is firstly gargled with clear water before being used, then the gargle is gargled with the gargle water for more than 1 minute, and the gargle is not gargled with the clear water after being gargled. The medicine is taken 4 times a day according to the physician's compliance and the specific condition, 20mL each time, and 3 days as a course of treatment. The treatment effect is counted after 3 days, and the judgment standard of the treatment effect is as follows:
the method has the following advantages: reduction or partial disappearance of symptoms of discomfort;
and (3) curing: the discomfort symptom disappears, and no recurrence occurs;
and (4) invalidation: the discomfort symptoms are unchanged or recur.
The results are shown in the following table:
TABLE 4 treatment Effect on halitosis with mouthwash
| Group of | Total number of cases | Age (age) | Number of cure cases | Effective number of cases | Number of invalid cases | Total effective rate |
| Experimental group | 200 | 18～65 | 128 | 68 | 4 | 93.00 |
| Control group | 200 | 18～65 | 96 | 69 | 35 | 82.50 |
From the test results of table 4, it can be seen that the treatment effect of the mouthwash of the present invention is significantly better than other products.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The antibacterial and anti-inflammatory composition is characterized by being prepared from the following raw materials in parts by weight:
3-10 parts of spider freeze-dried powder, 10-20 parts of honeysuckle, 8-12 parts of coptis chinensis, 25-35 parts of gypsum, 10-14 parts of rhizoma anemarrhenae, 4-8 parts of asarum, 10-20 parts of radix rehmanniae, 10-14 parts of cortex moutan, 10-20 parts of peony, 10-14 parts of radix scrophulariae, 8-12 parts of holy basil and 3-7 parts of jasmine;
the preparation method comprises the following steps: mixing raw materials of spider freeze-dried powder, honeysuckle, coptis chinensis, gypsum, rhizoma anemarrhenae, asarum, radix rehmanniae, cortex moutan, peony, radix scrophulariae, holy basil and jasmine, extracting with alcohol, and filtering to obtain the antibacterial and anti-inflammatory composition.
2. The bacteriostatic and anti-inflammatory composition according to claim 1, which is prepared from the following raw materials in parts by weight:
7 parts of spider freeze-dried powder, 15 parts of honeysuckle, 10 parts of coptis chinensis, 30 parts of gypsum, 12 parts of rhizoma anemarrhenae, 6 parts of asarum, 15 parts of radix rehmanniae, 12 parts of cortex moutan, 15 parts of peony, 12 parts of radix scrophulariae, 10 parts of holy basil and 5 parts of jasmine.
3. The bacteriostatic and anti-inflammatory composition according to claim 1, which is prepared from the following raw materials in parts by weight:
3 parts of spider freeze-dried powder, 10 parts of honeysuckle, 12 parts of coptis chinensis, 25 parts of gypsum, 14 parts of rhizoma anemarrhenae, 4 parts of asarum, 20 parts of radix rehmanniae, 10 parts of cortex moutan, 20 parts of peony, 10 parts of radix scrophulariae, 12 parts of holy basil and 3 parts of jasmine.
4. The bacteriostatic and anti-inflammatory composition according to claim 1, which is prepared from the following raw materials in parts by weight:
10 parts of spider freeze-dried powder, 20 parts of honeysuckle, 8 parts of coptis chinensis, 35 parts of gypsum, 10 parts of rhizoma anemarrhenae, 8 parts of asarum, 10 parts of radix rehmanniae, 14 parts of cortex moutan, 10 parts of peony, 14 parts of radix scrophulariae, 8 parts of holy basil and 7 parts of jasmine.
5. The method for preparing a bacteriostatic and anti-inflammatory composition according to any one of claims 1 to 4, wherein raw materials of freeze-dried spider powder, honeysuckle, coptis chinensis, gypsum, rhizoma anemarrhenae, asarum, radix rehmanniae, cortex moutan, peony, radix scrophulariae, holy basil and jasmine are mixed, extracted with alcohol and filtered to obtain the bacteriostatic and anti-inflammatory composition.
6. The preparation method according to claim 5, wherein the alcohol extraction is: soaking in ethanol water solution for 10-20 days.
7. The method according to claim 6, wherein the ethanol in the ethanol aqueous solution is 50 to 70 volume percent.
8. The preparation method according to claim 6 or 7, wherein the amount ratio of the raw material to the ethanol aqueous solution is (100-200) in g/mL: 1000.
9. an oral health care product comprising the bacteriostatic anti-inflammatory composition of any one of claims 1 to 4 and pharmaceutically or dietetically acceptable auxiliary materials.
10. The oral care product of claim 9, wherein the oral care product is in the form of a mouth freshener, chewing gum, toothpaste, tooth gel, tooth powder, mouthwash, or tablet.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010046291A2 (en) * | 2008-10-24 | 2010-04-29 | Henkel Ag & Co. Kgaa | Use of hydroxycinnamic acids and derivatives thereof and/or of plant extracts for treating body odor |
| CN102526597A (en) * | 2012-02-14 | 2012-07-04 | 张秀兰 | Medicament for treating ozostomia and preparation method thereof |
| CN104083662A (en) * | 2014-07-19 | 2014-10-08 | 陈鸿雁 | Mouthwash for eliminating ozostomia caused by stomatitis and preparation method of mouthwash |
| CN104688850A (en) * | 2013-12-04 | 2015-06-10 | 肖峰 | Traditional Chinese medicine mouthwash |
| CN107029021A (en) * | 2017-06-16 | 2017-08-11 | 刘晓梅 | It is a kind of to have oral nursing liquid of therapeutic efficiency and preparation method thereof concurrently |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010046291A2 (en) * | 2008-10-24 | 2010-04-29 | Henkel Ag & Co. Kgaa | Use of hydroxycinnamic acids and derivatives thereof and/or of plant extracts for treating body odor |
| CN102526597A (en) * | 2012-02-14 | 2012-07-04 | 张秀兰 | Medicament for treating ozostomia and preparation method thereof |
| CN104688850A (en) * | 2013-12-04 | 2015-06-10 | 肖峰 | Traditional Chinese medicine mouthwash |
| CN104083662A (en) * | 2014-07-19 | 2014-10-08 | 陈鸿雁 | Mouthwash for eliminating ozostomia caused by stomatitis and preparation method of mouthwash |
| CN107029021A (en) * | 2017-06-16 | 2017-08-11 | 刘晓梅 | It is a kind of to have oral nursing liquid of therapeutic efficiency and preparation method thereof concurrently |
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