CN107494228A - A kind of implantation methods of dendrobium candidum - Google Patents
A kind of implantation methods of dendrobium candidum Download PDFInfo
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- CN107494228A CN107494228A CN201710842172.7A CN201710842172A CN107494228A CN 107494228 A CN107494228 A CN 107494228A CN 201710842172 A CN201710842172 A CN 201710842172A CN 107494228 A CN107494228 A CN 107494228A
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- dendrobium candidum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
Abstract
The present invention relates to agricultural technology field, and in particular to a kind of implantation methods of dendrobium candidum, it is characterised in that：Concretely comprise the following steps：Nursery is carried out using Seeds of Dendrobium Candidum, dendrobium candidum after nursery is grown up reaches stem and transplanted after 5 6cm in carrying out binding plantation on trunk；The dendrobium candidum is being bundled on trunk first on the basis of the root system bottom of dendrobium candidum, is tied after using cushion of moss, dendrobium candidum root is surrounded using the rope made of hemp；The moss be by after some mosses vertically discharge and with the root parallel peripheral of dendrobium candidum around.This method is simple and easy, can improve that emergence rate, survival rate, tiller number are more, improves the polyoses content in dendrobium candidum, so as to lift the quality of dendrobium candidum, has higher economic value.
Description
【Technical field】
The present invention relates to agricultural technology field, and in particular to a kind of implantation methods of dendrobium candidum.
【Background technology】
Dendrobium candidum is also known as ribbed hedyotis herb, is the stem of the perennial herbaceous plant dendrobium candidum of growing nonparasitically upon another plant of orchid family Dendrobium, because of epidermis
Gained the name in iron green, be a kind of precious Wild Medicinal.Height above sea level is born in up on the dark and damp rock in 1600 meters of mountain region half,
The environment of warm and moist weather and half cloudy half light is liked, can not resist cold.Generally it is resistant to -5 DEG C of low temperature.The stem of noble dendrobium can be divided into yellow grass, gold
Tens of kinds of hairpin, horsewhip etc., dendrobium candidum are the superfine product of the stem of noble dendrobium.Dendrobium candidum has unique medical value, is used as medicine with its stem,
《Chinese Pharmacopoeia》Recording the stem of noble dendrobium has the effect of reinforcing stomach reg fluid, nourishing Yin and clearing heat.It is classical in the Tang Dynasty medical science《Taoist Scriptures》In, dendrobium candidum
" first of Chinese nine big celestial grass " are listed in, since the Tang and Song Dynasty, have been listed in royal tribute always.Dendrobium candidum is listed in " China nine
First of big celestial grass ", since the Tang and Song Dynasty, royal tribute has been listed in always.The main consumption market of the current skin stem of noble dendrobium of iron also mainly exists
Also there is seldom pin expense in the Zhejiang and the big city such as the band of Shanghai one, Beijing in China；It is exactly in addition Hongkong and Macro and south east asia, west
Also there is import in developed country from China for a long time in the past few decades, and in the last few years, with the propagation of TCM Culture, consumption figure also exists
Expand.
Implantation methods are many in recent years, and what is be widely adopted at present is " plastic greenhouse planting method ", this method, concentrates kind
Plant, be easy to manage, yield is high, good in economic efficiency, but builds greenhouse frame, and one-time investment cost is big, the palpus in planting process
The growing environment condition of the strict control stem of noble dendrobium, to promote its growth cycle to shorten, the solid hypertrophy of cane, or even the peasant household having are increasing
Add the excessive applying pesticides chemical fertilizer of yield, the result of many factors synergy so that though the dendrobium candidum yield of production is high interior
Wild Dendrodium is much worse than in quality, medicines and health protection effect also substantially reduces.And dendrobium candidum contain dendrobium polysaccharide, alkaloid,
The effective active compositions such as amino acid, luxuriant and rich with fragrance class compound, there is anti-oxidant, antitumor, reduction blood glucose, strengthen immunity, alleviate body
Power fatigue and clearing and other effects.In many active components of dendrobium candidum, dendrobium polysaccharide has very strongly active.Therefore, the stem of noble dendrobium
Polyoses content number turn into one of standard for judging that dendrobium candidum is fine or not.
【The content of the invention】
The goal of the invention of the present invention is：For it is above-mentioned the problem of, the present invention provides a kind of implantation methods of dendrobium candidum,
This method is simple and easy, can improve that emergence rate, survival rate, tiller number are more, the polyoses content in dendrobium candidum is improved, so as to carry
The quality of dendrobium candidum is risen, there is higher economic value.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of implantation methods of dendrobium candidum, specifically include following steps：Nursery is carried out using Seeds of Dendrobium Candidum, when educating
Dendrobium candidum after seedling is grown up reaches stem and transplanted after 5-6cm in carrying out binding plantation on trunk；The dendrobium candidum is being tied
It is tied on trunk first on the basis of the root system bottom of dendrobium candidum, fiber crops is used after dendrobium candidum root is surrounded using cushion of moss
Rope is tied；The moss be by after some mosses vertically discharge and with the root parallel peripheral of dendrobium candidum around.
Further illustrate, seedling medium is made up of the raw material of following percentage by weight used in the nursery：Expansion is precious
Pearl stone 15%-20%, rotten wood 10%-15%, cherry-kernel oil 10%-20%, cotton seed hulls 10%-15%, bagasse 10%-
15%th, fibre strength is 100-200g/d cocoanut husk fibers 10%-20%, the beans that viable count is the CFU/mL of 2.01-5.18 hundred million
Bag bacterium bacterium solution 1%-3%, the Fusarium oxysporum bacterium solution 5%-10% that viable count is the CFU/mL of 6.10-8.16 hundred million, viable count are
2.56-3.45 hundred million CFU/mL loudspeaker bracket fungus bacterium solution 3%-5%.
Further illustrate, the cherry-kernel oil is specifically what is be prepared by following methods：
By cherry benevolence use quality concentration to be dried after 10-15% saline sook, after being crushed under 18 degrees Celsius
100 sieves are crossed, are then 1 according to volume ratio by powder and petroleum ether：Apparatus,Soxhlet's is put into after 3-8 mixing to soak 10 hours, and
It is Celsius 70 after immersion 10 hours at interval of the use of microwave power being the 400MHz-500MHz microwave irradiation 20-30 seconds after 2 hours
Lower refluxing extraction 5-6 hours to be spent, ultrasonic assistant extraction is carried out during refluxing extraction, the frequency of ultrasonic wave is 300W-500W,
Petroleum ether extract is can obtain after refluxing extraction, rotary evaporator recovery petroleum ether is then passed through and removes moisture, obtain
Oily liquids, it is positioned over constant weight in the drying box under the vacuum of 1.2-1.5 standard atmospheric pressure, you can obtain cherry-kernel oil.
Further illustrate, contain 22-28% aliphatic acid in the cherry-kernel oil, the composition of aliphatic acid is by following weight percent
The composition of ratio：Oleic acid 42.138%, linoleic acid 32.012%, linseed oil 8.015%, palmitic acid 5.225%, aliphatic acid C18:0
2.156%th, eicosapentaenoic acid 2.421%, docosahexaenoic acid 3.228%, EPA-E C20：5
2.151%th, docosahexaenoic acid ethyl C22：6 2.654%.
Further illustrate, a diameter of 0.3-0.5mm of the rope made of hemp core of the rope made of hemp；The rope made of hemp is twisted by 2 slivers；Institute
The twist for stating the rope made of hemp is 5-8/100cm.
Further illustrate, the moss is to select fresh and alive moss；The long 3-6cm of black hair of the moss.
Further illustrating, the moss is that the harvesting in second day after raining uses, and the mud on moss cleans up,
The eliminating impurities of bulk；If the moss of harvesting is relatively more, shady and cool ground is laid in during storage, thickness is less than 5cm, works as moss
Moisturizing is wanted when comparing dry, to keep the vigor of moss, while also not allow ponding to soak moss for a long time.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
First, select the long 3-6cm of black hair moss and the root parallel peripheral of dendrobium candidum to be wrapped after one layer in of the invention
The root of the dendrobium candidum after nursery is wrapped, the general root system president 3-5cm of the dendrobium candidum after nursery, with the root of dendrobium candidum
On the basis of being bottom, moss may grow root by the stem of a part of dendrobium candidum also around but not influenceing iron sheet
The growth of the stem of noble dendrobium, by research find be on the contrary the moss surrounded grow root by a part dendrobium candidum stem also around
Growing way it is more preferable, tiller number is more；Wrap the thickness thickness 3-10mm of the moss of dendrobium candidum root, and moss vertically discharge with
Dendrobium candidum root is parallel vertically, is found by experimental study, and vertical parallel root parcel is compared at vertical parallel root parcel
Reason, the growing way of dendrobium candidum is more preferable, and survival rate is higher.Second, the application has used Seeds of Dendrobium Candidum to be bred, and is multiplied into
The dendrobium candidum of work(has with dendrobium officinale more similar composition composition and fast numerous obtained dendrobium candidum compared with effect
More preferably；Seed seedling-raising not only needs to control temperature, humidity, shines positive time etc., also needed to suitable seedling medium, improves seed
Emergence rate, the application carries out research specifically for seedling medium to be ensured to improve the emergence rate of seed；By the research of the applicant
It was found that the use of pisolithus tinctorius Coker et Coucs bacterium solution 1%-3% that viable count is the CFU/mL of 2.01-5.18 hundred million, viable count is the CFU/ of 6.10-8.16 hundred million
ML Fusarium oxysporum bacterium solution 5%-10%, 3%-5% pairs of the loudspeaker bracket fungus bacterium solution that viable count is the CFU/mL of 2.56-3.45 hundred million
Seed, which carries out nursery, can promote the sprouting of seed, improve germination rate, and three kinds of bacterium solution collective effects are in nursery later stage energy
Bagasse, cotton seed hulls, rotten wood is promoted to decompose a large amount of release energy elements, but energy element will excessively cause the phenomenon of burn seedlings, therefore
The pisolithus tinctorius Coker et Coucs of addition, loudspeaker bracket fungus bacterium solution not only discharge substantial amounts of heat in growth period and promote fermentation to decompose, and are growing
Journey, which can also discharge pisolithus tinctorius Coker et Coucs, the distinctive physiology of loudspeaker bracket fungus, chemical substance, nutriment etc., to promote Seeds of Dendrobium Candidum
Sprout, and the amount that dendrobium candidum absorbs energy element can be balanced, and the cocoanut husk fiber in matrix serves the ability of water conservation,
The nursery later stage, if the fertility of matrix will be lifted by decomposing the release energy element such as carbon nitrogen source by bacterium in the lump, therefore in order to ensure after
It is continuous to play the water holding capacity for keeping whole matrix, cause burn seedlings phenomenon without increase matrix fertility, the fibre strength used for
100-200g/d cocoanut husks fiber can play water retention always in nursery stage, up to dendrobium candidum seedling length to the long 5- of stem
Just start fermentation after 6cm and corrode molten disconnected decomposition, although expanded pearlite stone water absorption rate is high, poor water resistance, protected in the nursery later stage
Wet performance can reduce, therefore it is the Water-saving effect that 100-200g/d cocoanut husk fibers are ensured to add fibre strength.3rd,
The cherry-kernel oil added in seedling medium finds it after Seeds of Dendrobium Candidum sprouting rich in there is substantial amounts of aliphatic acid, by research
The content of fatty acid that can be improved in dendrobium candidum is absorbed, researcher also found that polyoses content can be with aliphatic acid in dendrobium candidum
The rise of content and increase, therefore, cherry-kernel oil can significantly improve polyoses content in dendrobium candidum.
【Embodiment】
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention,
It is not intended to limit the present invention.
Embodiment 1：
By cherry benevolence use quality concentration to be dried after 10% saline sook, mistake after being crushed under 18 degrees Celsius
100 sieves, it is then 1 according to volume ratio by powder and petroleum ether：Apparatus,Soxhlet's is put into after 3 mixing to soak 10 hours, and every
It was 400MHz microwave irradiations 20 seconds using microwave power after 2 hours, it is small in 70 degrees Celsius of lower refluxing extractions 5 after soaking 10 hours
When, ultrasonic assistant extraction is carried out during refluxing extraction, the frequency of ultrasonic wave is 300W, be can obtain after refluxing extraction
Petroleum ether extract, it is then passed through rotary evaporator recovery petroleum ether and removes moisture, obtain oily liquids, be positioned over 1.2 marks
Constant weight in drying box under the vacuum of quasi- atmospheric pressure, you can obtain cherry-kernel oil；Contain 22% aliphatic acid in cherry-kernel oil.
Embodiment 2：
By cherry benevolence use quality concentration to be dried after 15% saline sook, mistake after being crushed under 18 degrees Celsius
100 sieves, it is then 1 according to volume ratio by powder and petroleum ether：Apparatus,Soxhlet's is put into after 8 mixing to soak 10 hours, and every
It was 500MHz microwave irradiations 30 seconds using microwave power after 2 hours, it is small in 70 degrees Celsius of lower refluxing extractions 6 after soaking 10 hours
When, ultrasonic assistant extraction is carried out during refluxing extraction, the frequency of ultrasonic wave is 500W, be can obtain after refluxing extraction
Petroleum ether extract, it is then passed through rotary evaporator recovery petroleum ether and removes moisture, obtain oily liquids, be positioned over 1.5 marks
Constant weight in drying box under the vacuum of quasi- atmospheric pressure, you can obtain cherry-kernel oil；Contain 28% aliphatic acid in cherry-kernel oil.
Embodiment 3：
By cherry benevolence use quality concentration to be dried after 12% saline sook, mistake after being crushed under 18 degrees Celsius
100 sieves, it is then 1 according to volume ratio by powder and petroleum ether：Apparatus,Soxhlet's is put into after 5 mixing to soak 10 hours, and every
It was 450MHz microwave irradiations 25 seconds using microwave power after 2 hours, in 70 degrees Celsius of lower refluxing extractions 5.5 after soaking 10 hours
Hour, ultrasonic assistant extraction is carried out during refluxing extraction, the frequency of ultrasonic wave is 400W, can be obtained after refluxing extraction
To petroleum ether extract, it is then passed through rotary evaporator recovery petroleum ether and removes moisture, obtain oily liquids, be positioned over 1.3
Constant weight in drying box under the vacuum of standard atmospheric pressure, you can obtain cherry-kernel oil；Contain 25% aliphatic acid in cherry-kernel oil.
The composition of aliphatic acid in the cherry-kernel oil obtained by detecting embodiment 1-3 is by following percentage by weight
Composition：Oleic acid 42.138%, linoleic acid 32.012%, linseed oil 8.015%, palmitic acid 5.225%, aliphatic acid C18:0
2.156%th, eicosapentaenoic acid 2.421%, docosahexaenoic acid 3.228%, EPA-E C20：5
2.151%th, docosahexaenoic acid ethyl C22：6 2.654%.Embodiment 1-3 composition data difference is only the after decimal point
Ignored on four.
Embodiment 4：
A kind of implantation methods of dendrobium candidum, specifically include following steps：Nursery is carried out using Seeds of Dendrobium Candidum, when educating
Dendrobium candidum after seedling is grown up reaches stem in 5cm, the long 3cm of root system, transplants afterwards in carrying out binding plantation on trunk；The iron sheet
The stem of noble dendrobium is being bundled on trunk first on the basis of the root system bottom of dendrobium candidum, using a thickness 8mm mosses by dendrobium candidum root
Tied after surrounding using the rope made of hemp in portion；The moss be by some mosses vertically discharge after and with the root parallel peripheral of dendrobium candidum
Around；
Wherein, seedling medium used in nursery is made up of the raw material of following percentage by weight：It is expanded pearlite stone 15%, rotten
Wood 10%, cherry-kernel oil 20%, cotton seed hulls 15%, bagasse 15%, the fibre strength 100- being prepared by embodiment 1
Pisolithus tinctorius Coker et Coucs bacterium solution 2% that 200g/d cocoanut husks fiber 10%, viable count are 2.01CFU/mL, viable count are 6.10CFU/mL's
Fusarium oxysporum bacterium solution 10%, the loudspeaker bracket fungus bacterium solution 3% that viable count is 2.56CFU/mL.
Wherein, a diameter of 0.3mm of the rope made of hemp core of the rope made of hemp；The rope made of hemp is twisted by 2 slivers；The twist of the rope made of hemp is
5/100cm.
Wherein, moss is to select fresh and alive moss；The long 3cm of black hair of the moss.
Wherein, moss is that the harvesting in second day after raining uses, and the mud on moss cleans up, the debris of bulk
Remove；If the moss of harvesting is relatively more, the ground in cool place is laid in during storage, thickness is less than 5cm, is wanted when moss is more dry
Moisturizing, to keep the vigor of moss, while ponding is not also allowed to soak moss for a long time.
Embodiment 5：
Specific method is same as Example 4, and difference is the long 5-6cm of black hair of the moss.
Embodiment 6：
Specific method is same as Example 4, and difference is the long 4-5cm of black hair of the moss.
Embodiment 7：
Specific method is same as Example 4, difference be moss be by some mosses vertically discharge after and with iron sheet stone
The root of dry measure used in former times vertically surrounds.
Experiment one：
After the dendrobium candidum that embodiment 4-7 is obtained carries out migration process, 30 plants are planted on the trunk of every one tree, often
Curl on trunk is bundled between strain to be bundled on trunk, and keeps spraying watering 0.5-1 hours daily, is being bundled in
During trunk, the suitable dendrobium candidum seedling of stem length, stem diameter, stipes quantity is selected, embodiment 4-6 is according to above-mentioned side
Method is respectively bundled on the same trunk with a piece of woods, and each group is done three horizontal experiments, is carried out in October, 2015
Transplanting, in May, 2,016 the 90 of each embodiment are carried out from dendrobium candidum detecting sprout tillers number after the transfer, stem length, stem are thick, stipes
Number (being measured respectively with ruler and electronic digital indicator)；Each sprout tillers stipes number is counted, and calculates internode away from (stem length/stipes number
Amount)
Table 1 (average ± standard deviation n=3)
| Stem length/cm | Stem is thick/mm | Stipes number/ | Internode away from | Sprout tillers number/strain | Survival rate/% | |
| Embodiment 4 | 14.21±0.42 | 4.89±0.32 | 11.5±0.23 | 1.23±0.12 | 9.2±0.45 | 100% |
| Embodiment 5 | 17.43±0.31 | 6.05±0.13 | 14.1±0.12 | 1.24±0.16 | 12.4±0.32 | 100% |
| Embodiment 6 | 16.21±0.23 | 5.16±0.22 | 13.2±0.22 | 1.23±0.10 | 10.3±0.36 | 100% |
| Embodiment 7 | 11.13±0.12 | 3.95±0.12 | 9.2±0.11 | 1.21±0.10 | 7.2±0.34 | 92% |
As seen from the above table, find out in embodiment 4-5 contrast, the moss surrounded of use grows root by the iron of a part
Skin stem of noble dendrobium stem also around growing way it is more preferable, tiller number is more；From embodiment 4 compared with Example 7 compared with vertical parallel root
Parcel is handled compared to vertical parallel root parcel, and the growing way of dendrobium candidum is more preferable, and survival rate is higher；But internode away from difference not
Greatly, this may be relevant with dendrobium candidum nature, keeps fixed internode to keep good away from being more beneficial for being not broken and deposit
Person's character living.
Embodiment 8：
A kind of implantation methods of dendrobium candidum, specifically include following steps：Nursery is carried out using Seeds of Dendrobium Candidum, when educating
Dendrobium candidum after seedling is grown up reaches stem and transplanted after 6cm in carrying out binding plantation on trunk；The dendrobium candidum is bundling
In first on the basis of the root system bottom of dendrobium candidum, making on trunk after using a thickness 9mm mosses, dendrobium candidum root is surrounded
Tied with the rope made of hemp；The moss be by after some mosses vertically discharge and with the root parallel peripheral of dendrobium candidum around；
Wherein, seedling medium used in nursery is made up of the raw material of following percentage by weight：It is expanded pearlite stone 20%, rotten
Wood 15%, cherry-kernel oil 10%, cotton seed hulls 10%, bagasse 14%, the fibre strength 200g/d being prepared by embodiment 2
Pisolithus tinctorius Coker et Coucs bacterium solution 1% that cocoanut husk fiber 20%, viable count are 5.18 hundred million CFU/mL, the point that viable count is 8.16 hundred million CFU/mL
Spore Fusariumsp bacterium solution 5%, the loudspeaker bracket fungus bacterium solution 5% that viable count is 3.45 hundred million CFU/mL.
Wherein, a diameter of 0.5mm of the rope made of hemp core of the rope made of hemp；The rope made of hemp is twisted by 2 slivers；The twist of the rope made of hemp is
8/100cm.
Wherein, moss is to select fresh and alive moss；The long 6cm of black hair of the moss.
Wherein, moss is that the harvesting in second day after raining uses, and the mud on moss cleans up, the debris of bulk
Remove；If the moss of harvesting is relatively more, the ground in cool place is laid in during storage, thickness is less than 5cm, is wanted when moss is more dry
Moisturizing, to keep the vigor of moss, while ponding is not also allowed to soak moss for a long time.
Embodiment 9：
A kind of implantation methods of dendrobium candidum, specifically include following steps：Nursery is carried out using Seeds of Dendrobium Candidum, when educating
Dendrobium candidum after seedling is grown up reaches stem and transplanted after 5cm in carrying out binding plantation on trunk；The dendrobium candidum is bundling
In first on the basis of the root system bottom of dendrobium candidum, making on trunk after using a thickness 10mm mosses, dendrobium candidum root is surrounded
Tied with the rope made of hemp；The moss be by after some mosses vertically discharge and with the root parallel peripheral of dendrobium candidum around；
Wherein, seedling medium used in nursery is made up of the raw material of following percentage by weight：It is expanded pearlite stone 18%, rotten
Wood 13%, cherry-kernel oil 15%, cotton seed hulls 13%, bagasse 10%, the fibre strength 150g/d being prepared by embodiment 3
Pisolithus tinctorius Coker et Coucs bacterium solution 3% that cocoanut husk fiber 15%, viable count are 3.25 hundred million CFU/mL, the point that viable count is 7.05 hundred million CFU/mL
Spore Fusariumsp bacterium solution 8%, the loudspeaker bracket fungus bacterium solution 4% that viable count is 3.10 hundred million CFU/mL.
Wherein, a diameter of 0.3-0.5mm of the rope made of hemp core of the rope made of hemp；The rope made of hemp is twisted by 2 slivers；The sth. made by twisting of the rope made of hemp
Spend for 5-8/100cm.
Wherein, moss is to select fresh and alive moss；The long 3-6cm of black hair of the moss.
Wherein, moss is that the harvesting in second day after raining uses, and the mud on moss cleans up, the debris of bulk
Remove；If the moss of harvesting is relatively more, the ground in cool place is laid in during storage, thickness is less than 5cm, is wanted when moss is more dry
Moisturizing, to keep the vigor of moss, while ponding is not also allowed to soak moss for a long time.
Embodiment 10：
Method is same as Example 9, and difference is that the matrix formulations of nursery are different：It is not added with cherry-kernel oil.
Embodiment 11：
Method is same as Example 9, and difference is that the matrix formulations of nursery are different：Expanded pearlite stone 18%, rotten wood 12%,
The cherry-kernel oil 15% that is prepared by embodiment 3, cotton seed hulls 13%, bagasse 18%, fibre strength are outside 150g/d coconuts
Case fibre 20%.
Embodiment 12：
Method is same as Example 9, and difference is that the matrix formulations of nursery are different：Expanded pearlite stone 20%, rotten wood 18%,
Cherry-kernel oil 15%, cotton seed hulls 16%, bagasse 15%, the viable count being prepared by embodiment 3 are 3.25 hundred million CFU/mL's
Fusarium oxysporum bacterium solution 8% that pisolithus tinctorius Coker et Coucs bacterium solution 3%, viable count are 7.05 hundred million CFU/mL, viable count are 3.10 hundred million CFU/mL
Loudspeaker bracket fungus bacterium solution 4%.
Experiment two：Seed is subjected to nursery by embodiment 8-12 method, cultivated in seedling medium, during culture
Nursed young plants in hothouses under the identical temperature humidity environment identical with air mass flow, each embodiment sows 120
Seed is initial just after planting to pour immediately permeable in respective nursery seedling medium, allows seed to enter seedling medium with current
It is interior, nursery processing is proceeded by, subsequent every 5 days detection humidity, it is 70% ± 5% suitably to add water to keep seedling medium humidity,
The humidity change (the humidity average for calculating 6 times) of detection is recorded respectively, and nursery records emergence rate, is recorded as after the 60th day after 30 days
Seedling rate, is shown in Table 2；The stem length of after seedling, stem diameter, the suitable dendrobium candidum of stipes quantity are chosen to, October 18 in 2015
Day is transplanted, and the dendrobium candidum seedling that each embodiment is chosen is bundled on the same trunk with a piece of woods respectively, every
30 plants are planted on the trunk of one tree, curl on trunk is bundled between every plant and is bundled on trunk, and is kept daily
Spraying watering 0.5-1 hours, each group is done three horizontal experiments, on May 28th, 2016 by the 90 of each embodiment from iron sheet
The stem of noble dendrobium is detected, and weighs total fresh weight, stem fresh weight and leaf fresh weight, with phenol-dense sulphuric acid method and version in 2010《Chinese Pharmacopoeia》It is attached
VA measure polysaccharide (in terms of DEXTROSE ANHYDROUS, hereinafter referred to as polysaccharide) and extract content are recorded, is shown in Table 3.
Table 2 (average ± standard deviation n=3)
As seen from the above table, find out in embodiment 8-12 contrast, just using expanded pearlite stone water holding capacity not as addition
Fibre strength is the water holding capacity after 100-200g/d cocoanut husk fibers, and the humidity of embodiment 12 is recited as respectively 70
± 0.6%, 67 ± 0.5%, 60 ± 0.3%, 55 ± 0.7%, 53 ± 0.5%, 50 ± 0.4%, also demonstrate over time
The water holding capacity of passage expanded pearlite stone worse and worse, but with the addition of fibre strength as after 100-200g/d cocoanut husk fibers
Water holding capacity will increase, and fibre strength is that 100-200g/d cocoanut husk fibers are not easy to be decomposed during nursery,
Water holding capacity is maintained, the humidity detected every time is more homogeneous.
By embodiment 8-10 compared with embodiment 11, it is porous to be not added with pisolithus tinctorius Coker et Coucs bacterium solution, Fusarium oxysporum bacterium solution, loudspeaker
Bacterium bacterium solution, the emergence rate for not only influenceing dendrobium candidum have an effect on planting percent, seed can be promoted to sprout under conditions of bacterium solution, carried
High planting percent, and the application uses 120 seed seedling-raisings so that at least each embodiment has 100 seed seedlings to move
Plant continues to test.
In bacterium solution and fibre strength it is 150g/d cocoanut husks it can be seen from embodiment 9, embodiment 11, embodiment 12
Under conditions of fiber, the planting percent of dendrobium candidum increases, organic in raw material can be decomposed under conditions of having bacterium solution first
Thing discharges the energy such as substantial amounts of carbon source nitrogen source element, if energy element excessive concentration, can cause burn seedlings, reduces planting percent, and decompose
It is that capacity of decomposition is stronger backward over time, because bacterium solution can grow into edible mushroom, edible mushroom can discharge during physiological growth
More chemical substances promote fermentation to decompose；And if fibre strength is not present for 150g/d cocoanut husk fibers, water holding capacity
It is not strong, bagasse, cotton seed hulls, rotten wood after corrosion can easy deposit moisture, excess moisture will also result in the butt rot of seedling,
Reduce planting percent.
Comparative example：The dendrobium candidum seedling of market buying, directly in 30 plants of trunk upper shed spiral planting stock, totally three groups,
Adhere to moss in opening, the trunk of the group is also with embodiment the same as a piece of woods.
Table 3 (average ± standard deviation n=3)
| Embodiment | Polyoses content/% | Total fresh weight/gm-2 | Stem fresh weight/gm-2 | Stem fresh weight/gm-2 |
| 8 | 39.25±1.22 | 485.13±12.25 | 384.12±11.25 | 101.01±1.02 |
| 9 | 35.46±1.06 | 454.16±14.91 | 354.12±14.02 | 100.04±0.89 |
| 10 | 35.04±2.11 | 416.51±12.65 | 324.41±12.07 | 92.10±0.58 |
| 11 | 35.12±1.26 | 404.11±18.07 | 312.14±17.20 | 91.97±0.87 |
| 12 | 35.20±1.14 | 393.04±12.02 | 302.05±11.24 | 90.99±0.88 |
| Comparative example | 21.11±1.02 | 305.40±11.17 | 221.26±10.21 | 84.14±0.96 |
As seen from the above table, carrying out nursery using the high cherry-kernel oil of content of fatty acid promotes seedling to contain substantial amounts of aliphatic acid,
The high polyoses content of content of fatty acid can increase therewith, and the species of the aliphatic acid in cherry-kernel oil is any on earth or which kind
Facilitation is served, it is also to be studied；And cherry-kernel oil can provide bacterium solution strain energy, strain vitality is enlivened, promotes kind
Son is sprouted.Stem is more than blade after planting 1 year, and blade is significantly less than stem weight again, using the overall technical architecture kind of the application
The dendrobium candidum for planting harvesting is advantageous on stem fresh weight, stem fresh weight, total fresh weight.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
- A kind of 1. implantation methods of dendrobium candidum, it is characterised in that：Concretely comprise the following steps：Nursery is carried out using Seeds of Dendrobium Candidum, Dendrobium candidum after nursery is grown up reaches stem and transplanted after 5-6cm in carrying out binding plantation on trunk；The dendrobium candidum It is being bundled on trunk first on the basis of the root system bottom of dendrobium candidum, is making after dendrobium candidum root is surrounded using cushion of moss Tied with the rope made of hemp；The moss be by after some mosses vertically discharge and with the root parallel peripheral of dendrobium candidum around.
- A kind of 2. implantation methods of dendrobium candidum according to claim 1, it is characterised in that：Educated used in the nursery Seedling matrix is made up of the raw material of following percentage by weight：Expanded pearlite stone 15%-20%, rotten wood 10%-15%, cherry-kernel oil 10%-20%, cotton seed hulls 10%-15%, bagasse 10%-15%, fibre strength are 100-200g/d cocoanut husk fibers Pisolithus tinctorius Coker et Coucs bacterium solution 1%-3%, the viable count that 10%-20%, viable count are the CFU/mL of 2.01-5.18 hundred million are 6.10-8.16 hundred million CFU/mL Fusarium oxysporum bacterium solution 5%-10%, the loudspeaker bracket fungus bacterium solution 3%- that viable count is the CFU/mL of 2.56-3.45 hundred million 5%.
- A kind of 3. implantation methods of dendrobium candidum according to claim 2, it is characterised in that：The cherry-kernel oil is specifically It is prepared by following methods：By cherry benevolence use quality concentration to be dried after 10-15% saline sook, 100 are crossed after being crushed under 18 degrees Celsius Sieve, it is then 1 according to volume ratio by powder and petroleum ether：Apparatus,Soxhlet's is put into after 3-8 mixing to soak 10 hours, and at interval of The use of microwave power it is the 400MHz-500MHz microwave irradiation 20-30 seconds after 2 hours, after immersion 10 hours at 70 degrees Celsius next time Stream extraction 5-6 hours, ultrasonic assistant extraction is carried out during refluxing extraction, the frequency of ultrasonic wave is 300W-500W, and backflow carries Petroleum ether extract is can obtain after taking, rotary evaporator recovery petroleum ether is then passed through and removes moisture, obtain oily liquid Body, it is positioned over constant weight in the drying box under the vacuum of 1.2-1.5 standard atmospheric pressure, you can obtain cherry-kernel oil.
- A kind of 4. implantation methods of dendrobium candidum according to claim 2, it is characterised in that：Contain in the cherry-kernel oil 22-28% aliphatic acid, the composition of aliphatic acid by following percentage by weight composition：Oleic acid 42.138%, linoleic acid 32.012%, Linseed oil 8.015%, palmitic acid 5.225%, aliphatic acid C18:0 2.156%, eicosapentaenoic acid 2.421%, 22 carbon Acid 3.228%, EPA-E C20：5 2.151%, docosahexaenoic acid ethyl C22：6 2.654%.
- A kind of 5. implantation methods of dendrobium candidum according to claim 1, it is characterised in that：The rope made of hemp core of the rope made of hemp A diameter of 0.3-0.5mm；The rope made of hemp is twisted by 2 slivers；The twist of the rope made of hemp is 5-8/100cm.
- A kind of 6. implantation methods of dendrobium candidum according to claim 1, it is characterised in that：The moss is from fresh and alive Moss；The long 3-6cm of black hair of the moss.
- A kind of 7. implantation methods of dendrobium candidum according to claim 1, it is characterised in that：The moss is to rain What is used plucked in second day afterwards, and the mud on moss cleans up, the eliminating impurities of bulk；If the moss of harvesting is relatively more, deposit The ground in cool place is laid in when putting, thickness is less than 5cm, moisturizing is wanted when moss is more dry, to keep the vigor of moss, simultaneously Also ponding is not allowed to soak moss for a long time.
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