CN107485627A - A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour - Google Patents
A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour Download PDFInfo
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- CN107485627A CN107485627A CN201710652686.6A CN201710652686A CN107485627A CN 107485627 A CN107485627 A CN 107485627A CN 201710652686 A CN201710652686 A CN 201710652686A CN 107485627 A CN107485627 A CN 107485627A
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- Prior art keywords
- sesame
- glossy ganoderma
- ganoderma extract
- composite glossy
- reinforced immunological
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Polymers & Plastics (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to traditional Chinese medicine and field of health care food, more particularly to a kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour.Composite glossy ganoderma extract of the present invention is the oral formulations being prepared by ganoderma lucidum, purple sesame, red sesame, the small pore fungi of brown sesame, Huang Zhi and rainbow conk through modern crafts means, preferably capsule, tablet and granule.Confirmed through the test of pesticide effectiveness, composite glossy ganoderma extract of the present invention has significant reinforced immunological and suppresses tumor effect, in addition, composite glossy ganoderma extract of the present invention can also slow down cancer pain, be obviously improved the quality of life of cancer patient, and product processes of the present invention are simple, each raw material is native fungal, takes safety, moderate product price, easily received by consumers in general.
Description
Technical field
The present invention relates to traditional Chinese medicine and field of health care food, more particularly to a kind of reinforced immunological and the compound spirit for suppressing tumour
Sesame extract.
Background technology
At present, ganoderma lucidum or ganoderma lucidum are contained in many medicines and health products with reinforced immunological power and function of tumor inhibition
Extract constituents.A kind of for example, application for a patent for invention " production method of ganoderma lucidum complex immunity agent "
(CN201710083299.5) a kind of method that ganoderma lucidum complex immunity agent is prepared using fermentation technique is disclosed in, by ganoderma lucidum and Huang
Ganoderma lucidum complex immunity agent is made in stilbene powder compatibility.Patent of invention " a kind of ganoderma particles of strengthen immunity and preparation method thereof "
(CN2014103240986) a kind of ganoderma particles of strengthen immunity and preparation method thereof are disclosed in, the ganoderma particles are with party
Ginseng, the Radix Astragali, ganoderma lucidum, the fruit of Chinese wolfberry and jujube are main active, are formulated by auxiliary material of dextrin, have enhancing immunity of organism
The effect of ability.Patent of invention " anticancer compound ganoderma composition, its purposes and include its pharmaceutical composition "
(CN201010606323.7) a kind of anticancer compound ganoderma composition is disclosed in, it is multiple by restructuring cephalotaxine hydrochloride, restructuring
Fang Zishan alcohol hydrochlorides, restructuring camplotheca acuminata China fir alkali salt hydrochlorate, compound ganoderma GL-PP enzyme, coriolan protease, selenium-rich polysaccharide
Protease, the dried venom of toads, Moschus, cow-bezoar and compound protease are made, and available for immunologic function is strengthened, various cancers are treated, for controlling
The malignant tumour treat, mitigated, eliminate each internal organs of body, mitigate the side effect of cancer chemotherapy.
However, above-mentioned strengthen immune, antineoplastic product still with place of some shortcomings using ganoderma lucidum is manufactured.Such as some
Product needs to prepare by zymotechnique, and production technology is complex, requires more harsh to equipment and processing conditions.Some productions
Product combine for a variety of Chinese medicine composition, and the relative amount of active component is relatively low in ganoderma lucidum, so as to have impact on its final enhancing
Immune and anticancer effect.Multiple protein enzyme and precious Chinese medicine are also employed in some products as raw material, first to producing, transporting
Very high requirement is proposed with preservation condition, on the other hand production cost is also significantly improved, makes the price of product very high,
Beyond the consuming capacity of many patients, product is difficult to promote the use of.
In addition, the enhancing combined completely by all kinds of fungi components compatibilities is immune, antineoplastic product, protected in current medicine
Strong product in the market remains difficult to see.
In consideration of it, the present invention has been prepared a kind of completely by multiclass fungi composition on the basis of compatibility mechanism repeatedly
The enhancing combined is immunized, suppresses tumour product.Reinforced immunological of the present invention and the composite glossy ganoderma extract production for suppressing tumour
Technique is simple, requires no fermentation process, by solvent extraction and the combination of superfine communication technique, can efficiently and completely carry
Take out the active component in each raw material.In addition, each raw material of the present invention is native fungal, safety is taken, moderate product price, easily
Received by consumers in general.Therefore, as a kind of reinforced immunological and suppression tumour safely, effectively, suitable for long-term use
Composite glossy ganoderma extract, popularization and application of the invention are expected to produce good social and economic benefit.
The content of the invention
Ganoderma lucidum (Ganoderma Lucidum Karst) is the fructification of On Polyporaceae ganoderma lucidum.With invigorating qi for tranquilization,
Relieving cough and asthma, the effect of promoting longevity, cure mainly the diseases such as dizziness egersis, palpitation, neurasthenia, consumptive disease cough and asthma.Modern study
It was found that the GL-B and Ganoderma lucidum triterpenes components that contain in ganoderma lucidum can strengthen the function of immune system, promotion lymph is shown
Cell is bred, and improves macrophage, NK cells, the phagocytic activity of T cell and lethality.The low or mistake of autoimmune function
Adjust, be the major reason of tumour occurrence and development, ganoderma lucidum can promote monokaryon macrophage thin by promoting the generation of interleukin 2
The phagocytic function of born of the same parents, the leucocyte level in the hematopoietic potential especially blood of human body is lifted, while utilize its active component pair
The inhibitory action of tumour cell, so as to as a kind of preferably tumor prevention and medicine.Meanwhile ganoderma lucidum does not almost have to human body
There is any toxic side effect, use is very safe, this advantage exactly many tumor chemotherapeutic drugs and other immunopotentiating agents institute
Do not possess.In addition, GL-B also has carcinogen in anti-aging, anti anoxia, hypotensive, hypoglycemic, promotion intestines and stomach
The effect such as discharge.The triterpenes components such as ganoderic acid also have analgesic, it is calm, suppress histamine release, liver protection, removing toxic substances, reducing blood lipid,
Improve microcirculation, suppress platelet aggregation, avoid vascular sclerosis etc. from acting on.
It was found that the antitumaous effect of ganoderma lucidum is mainly realized by following approach：(1) reinforced immunological power, induction cancer are thin
Born of the same parents are voluntarily dead；(2) propagation and the transfer of cancer cell are suppressed；(3) side effect of chemotherapy and radiation is mitigated；(4) alleviate cancer to suffer from
The distinctive pain of person；(5) it is cancer to prevent the cancerations such as ulcer, polyp, benign tumour；(6) prevent, avoid the recurrence of cancer.
Purple sesame, is the fructification of On Polyporaceae purple sesame, the strong intelligence of tool bowl spares, the effect of calming heart benefit stomach, cure mainly neurasthenia,
The diseases such as insomnia, stomachache, indigestion, also there is certain antitumaous effect.
Red sesame, it is the fructification of On Polyporaceae red sesame, is usually used in treating neurasthenia, giddy, insomnia, hepatitis, branch
The diseases such as san bronchial asthma.Modern pharmacology research confirms that red sesame is in enhancing body immunity, regulation blood glucose, control blood pressure, auxiliary
Tumor chemoradiotherapy, liver protecting, sleep etc. is promoted to be respectively provided with significant curative effect.
The brown small pore fungi of sesame, is a kind of On Polyporaceae, is often born in broad leaf tree rotten wood, currently for the small pore fungi of brown sesame
Pharmacological research is less, using its manufactured medicine and health products also there is not yet.
Huang Zhi, it is the fructification of Polyporaceae Sulfur bracket fungus, there is the effect of immune enhancing, strengthening spleen and nourishing stomach.
Rainbow conk, it is the drying fructification of On Polyporaceae Corilus versicolor Quel., there is the work(such as heat-clearing, removing toxic substances, anti-inflammatory, liver protection
Effect.The coriolan extracted from prepared from coriolus versicolor mycelium has that strong suppression is carcinous, to S180 sarcoma, Ehrlich tumor and
Gland cancer 755 has inhibitory action.We are processed using ultramicro grinding technique to rainbow conk, are advantageous to more completely retain it
In active component, the loss of functional component in solvent extracting process is avoided, moreover, ultra-micro powder can improve human body to work
The absorptivity of property composition.
The present invention passes through multiple compatibility mechanism, have developed the good reinforcing of a kind of significant effect, property easy to use, safe and exempts from
Epidemic disease and the composite glossy ganoderma extract for suppressing tumour.
Reinforced immunological of the present invention and the composite glossy ganoderma extract for suppressing tumour, it is characterised in that it is by following weight parts
Made of raw material：
Ganoderma lucidum 10-15；Purple sesame 6-12；Red sesame 6-9；
The brown small pore fungi 6-9 of sesame；Yellow sesame 6-9；Rainbow conk 4-8.
Preferably, the parts by weight of above-mentioned each raw material are：
Ganoderma lucidum 10；Purple sesame 6；Red sesame 6；
The brown small pore fungi 6 of sesame；Yellow sesame 6；Rainbow conk 4.
Preferably, the parts by weight of above-mentioned each raw material are：
Ganoderma lucidum 12；Purple sesame 8；Red sesame 8；
The brown small pore fungi 8 of sesame；Yellow sesame 8；Rainbow conk 6.
Preferably, the parts by weight of above-mentioned each raw material are：
Ganoderma lucidum 15；Purple sesame 12；Red sesame 9；
The brown small pore fungi 9 of sesame；Yellow sesame 9；Rainbow conk 8.
Preferably, reinforced immunological of the present invention and the composite glossy ganoderma extract of suppression tumour are prepared by the following method and formed：
(1) ganoderma lucidum, purple sesame, red sesame, the small pore fungi of brown sesame and Huang Zhi medicinal materials are mixed, water rinses, dry, adds 8-10 after crushing
30% ethanol solution of amount again, after soaking 2 hours, refluxing extraction 1 hour, decoction filtration, filtrate is standby, under the same conditions by medicine
Slag repeats extraction 1 time, filtering, merging filtrate, 10 times of amount ethanol is added into gained filter residue：Ethyl acetate=1：3 mixed solutions,
Refluxing extraction 1 hour, filtering, gained filtrate merge with foregoing filtrate, are spray-dried after being concentrated under reduced pressure into small size, must be sprayed
Mist xeraphium；
(2) ultramicro grinding will be carried out after the rinsing of rainbow conk medicinal material water, drying, gained ultra-micro powder obtains after crossing 300-500 mesh sieves
To rainbow conk Ultramicro-powder；
(3) above-mentioned spray dried powder and rainbow conk Ultramicro-powder are mixed, fully mixed in mixer, 20-40 is crossed after granulation
Mesh sieve, dry, whole grain, composite glossy ganoderma extract is produced after sterilization.
Further, capsule, piece are prepared by this area routine by composite glossy ganoderma extract made from the above method
Agent or the method for granule, the agent of composite glossy ganoderma extract capsule, tablet or granule is further made.
Moreover, it relates to a kind of reinforced immunological and the composite glossy ganoderma extract of suppression tumour are preparing antineoplastic
Application in thing.
The invention further relates to a kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour to prepare anti-tumor health care product
In application.
The invention further relates to a kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour to prepare raising immunity medicine
Application in thing.
The invention further relates to a kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour to improve immune try hard to keep in preparation
Application in strong product.
Confirmed through the test of pesticide effectiveness, reinforced immunological of the present invention and the composite glossy ganoderma extract for suppressing tumour have significant strengthen
Immune and suppression tumor effect, in addition, composite glossy ganoderma extract of the present invention can also slow down cancer pain, be obviously improved cancer patient
Quality of life, and composite glossy ganoderma of the present invention extraction raw material be all from natural drug, each raw material dosage meets States Pharmacopoeia specifications
And traditional medication specification, it is long-term use of safe and reliable.
Embodiment
Illustrate embodiments of the present invention below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through specific realities different in addition
The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under the spirit of the present invention.
Before the specific embodiment of the invention is further described, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment；It is also understood that the term used in the embodiment of the present invention is specific specific in order to describe
Embodiment, the protection domain being not intended to be limiting of the invention.
When embodiment provides number range, it should be appreciated that except non-invention is otherwise noted, two ends of each number range
Any one numerical value can be selected between point and two end points.Unless otherwise defined, in the present invention all technologies for using and
Scientific terminology is identical with the meaning that those skilled in the art of the present technique are generally understood that.Except used in embodiment specific method, equipment,
Outside material, according to grasp of the those skilled in the art to prior art and the record of the present invention, it can also use and this
Any method, equipment and the material of the similar or equivalent prior art of method, equipment described in inventive embodiments, material come real
The existing present invention.
Embodiment 1
A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour, are made of the raw material of following weight parts：
Ganoderma lucidum 10；Purple sesame 6；Red sesame 6；
The brown small pore fungi 6 of sesame；Yellow sesame 6；Rainbow conk 4.
Preparation method is as follows：
(1) ganoderma lucidum, purple sesame, red sesame, the small pore fungi of brown sesame and Huang Zhi medicinal materials are mixed, water rinses, dry, adds 10 times after crushing
30% ethanol solution is measured, after soaking 2 hours, refluxing extraction 1 hour, decoction filtration, filtrate is standby, under the same conditions by the dregs of a decoction
Extraction 1 time is repeated, filtering, merging filtrate, 10 times of amount ethanol are added into gained filter residue：Ethyl acetate=1：3 mixed solutions, return
Stream extraction 1 hour, filtering, gained filtrate merges with foregoing filtrate, is spray-dried after being concentrated under reduced pressure into small size, must be sprayed
Xeraphium；
(2) ultramicro grinding will be carried out after the rinsing of rainbow conk medicinal material water, drying, gained ultra-micro powder obtains cloud after crossing 400 mesh sieves
Sesame Ultramicro-powder；
(3) above-mentioned spray dried powder and rainbow conk Ultramicro-powder are mixed, fully mixed in mixer, 30 mesh are crossed after granulation
Sieve, dry, whole grain, the extract capsule agent of this composite glossy ganoderma is made by the conventional method for preparing capsule in this area after sterilization.
Embodiment 2
A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour, are made of the raw material of following weight parts：
Ganoderma lucidum 12；Purple sesame 8；Red sesame 8；
The brown small pore fungi 8 of sesame；Yellow sesame 8；Rainbow conk 6.
Preparation method is as follows：
(1) ganoderma lucidum, purple sesame, red sesame, the small pore fungi of brown sesame and Huang Zhi medicinal materials are mixed, water rinses, dry, adds 8 times after crushing
30% ethanol solution is measured, after soaking 2 hours, refluxing extraction 1 hour, decoction filtration, filtrate is standby, under the same conditions by the dregs of a decoction
Extraction 1 time is repeated, filtering, merging filtrate, 10 times of amount ethanol are added into gained filter residue：Ethyl acetate=1：3 mixed solutions, return
Stream extraction 1 hour, filtering, gained filtrate merges with foregoing filtrate, is spray-dried after being concentrated under reduced pressure into small size, must be sprayed
Xeraphium；
(2) ultramicro grinding will be carried out after the rinsing of rainbow conk medicinal material water, drying, gained ultra-micro powder obtains cloud after crossing 500 mesh sieves
Sesame Ultramicro-powder；
(3) above-mentioned spray dried powder and rainbow conk Ultramicro-powder are mixed, fully mixed in mixer, 40 mesh are crossed after granulation
Sieve, dry, whole grain, this composite glossy ganoderma extract tablet is made by the conventional method for preparing tablet in this area after sterilization.
Embodiment 3
A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour, are made of the raw material of following weight parts：
Ganoderma lucidum 15；Purple sesame 12；Red sesame 9；
The brown small pore fungi 9 of sesame；Yellow sesame 9；Rainbow conk 8.
Preparation method is as follows：
(1) ganoderma lucidum, purple sesame, red sesame, the small pore fungi of brown sesame and Huang Zhi medicinal materials are mixed, water rinses, dry, adds 10 times after crushing
30% ethanol solution is measured, after soaking 2 hours, refluxing extraction 1 hour, decoction filtration, filtrate is standby, under the same conditions by the dregs of a decoction
Extraction 1 time is repeated, filtering, merging filtrate, 10 times of amount ethanol are added into gained filter residue：Ethyl acetate=1：3 mixed solutions, return
Stream extraction 1 hour, filtering, gained filtrate merges with foregoing filtrate, is spray-dried after being concentrated under reduced pressure into small size, must be sprayed
Xeraphium；
(2) ultramicro grinding will be carried out after the rinsing of rainbow conk medicinal material water, drying, gained ultra-micro powder obtains cloud after crossing 300 mesh sieves
Sesame Ultramicro-powder；
(3) above-mentioned spray dried powder and rainbow conk Ultramicro-powder are mixed, fully mixed in mixer, 20 mesh are crossed after granulation
Sieve, dry, whole grain, the extract particles agent of this composite glossy ganoderma is made by the conventional method for preparing granule in this area after sterilization.
Comparative example
A kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour, are made of the raw material of following weight parts：
Ganoderma lucidum 44；Rainbow conk 6.
Preparation method is as follows：
(1) ganoderma lucidum medicinal material water is rinsed, dried, adding 8 times of 30% ethanol solutions of amount after crushing, after soaking 2 hours, backflow carries
Take 1 hour, decoction filtration, filtrate is standby, and the dregs of a decoction are repeated into extraction 1 time, filtering, merging filtrate, to gained under the same conditions
10 times of amount ethanol are added in filter residue：Ethyl acetate=1：3 mixed solutions, refluxing extraction 1 hour, filtering, gained filtrate with it is foregoing
Filtrate merges, and is spray-dried after being concentrated under reduced pressure into small size, obtains ganoderma lucidum spray dried powder；
(2) ultramicro grinding will be carried out after the rinsing of rainbow conk medicinal material water, drying, gained ultra-micro powder obtains cloud after crossing 500 mesh sieves
Sesame Ultramicro-powder；
(3) above-mentioned ganoderma lucidum spray dried powder and rainbow conk Ultramicro-powder are mixed, fully mixed in mixer, 40 are crossed after granulation
Mesh sieve, dry, whole grain, composite glossy ganoderma extract tablet is made by the conventional method for preparing tablet in this area after sterilization.
Efficacy test
(1) Evaluation of Immunity is strengthened
1st, subjects and packet
Experimental animal：BALB/c mouse, 18-22g, male, 40.Four groups are randomly divided into, 1 group of embodiment, embodiment 2
Group, comparative example group and blank control group, every group of 10 mouse.
2nd, test method
The feeding method of animal：Daily every of test group gavages composite glossy ganoderma extract (embodiment 1, embodiment 2 and contrast
Example product) 0.5ml (containing active ingredient 40mg)；Blank control group gavages androgynous ponding, continuous feeding 45 days.
Test principle：Mother cell breeder reaction occurs after T lymphocyte receptors ConA is stimulated, living cells is particularly bred carefully
MTh (a kind of flaxen azoles nitrogen salt) can be decomposed into blue purple crystal by the mitochondria hydrolase in born of the same parents, and its OD value can be anti-
Reflect the proliferative conditions of cell.
Instrument and material：RPMI1640 cell culture fluids, calf serum, 2 mercapto ethanol, penicillin, streptomysin, sword bean
Albumin A (ConA), hydrochloric acid, isopropanol, MTT, Hank, s liquid, PBS (PH7.2-7.4)；Gauze or 200 eye mesh screens, 24
Well culture plate, 96 well culture plates (flat), operating theater instruments, CO2gas incubator, ELIASA, 721 spectrophotometers, ultra-clean work
Make platform, autoclave, sterile filters.
3rd, test procedure
(1) preparation of reagents
Complete culture solution：RPMI1640 cell culture fluid filtration sterilizations, with 10% calf serum of preceding addition, 1% glutamy
Amine (200mmol/L), penicillin (100U/ml), streptomysin (100 μ g/L) and 5 × 10-5Mol/L 2 mercapto ethanol, use are sterile
1mol/L HCl or 1mol/L NaOH regulation PH to 7.0-7.2, that is, obtain complete culture solution.
ConA liquid：100 μ g/ml solution, filtration sterilization, in (- 20 DEG C) preservations of low temperature refrigerator are configured to distilled water.
Sterile Hank, s liquid：With preceding with 3.5% sterile NaHCO3Adjust PH to 7.2-7.4.
MTT liquid：5mg MTT are dissolved in 1ml PH7.2 PBS, it is now with the current.
Acid isopropyl alcoholic solution：4ml 1mol/L HCl, prepared before use are added in 96ml isopropanols.
(2) prepared by splenocyte suspension
It is sterile to take spleen, it is placed in and fills appropriate sterile Hank, in s liquid plates, gently ground spleen with tweezers, is made single thin
Born of the same parents' suspension.Spleen is ground through 200 mesh sieve net filtrations, or with 4 layers of gauze, with Hank, s liquid is washed 2 times, centrifuges 10min every time
(1000r/min).Then cell is suspended in 1ml complete culture solution, (should be with platform phenol orchid dyeing counting viable count
More than 95%) it is 3 × 10, to adjust cell concentration6Individual/ml.(3) lymphproliferation response
Holes will be divided to add in 24 well culture plates per a splenocyte suspension, per hole 1ml, wherein a hole adds 75 μ l ConA
Liquid (equivalent to 7.5 μ g/ml), another hole are placed in 5%CO as control2, 72h is cultivated in 37 DEG C of incubators.Culture terminates preceding 4h,
Supernatant 0.7ml is gently sucked per hole, 0.7ml is added and is free of the RPMI1640 nutrient solutions of calf serum, while add MTT
(5mg/ml) 50 μ l/ holes, continue to cultivate 4h.After culture terminates, 1ml acid isopropyl alcohol is added per hole, piping and druming mixes, and makes purple knot
Crystalline substance is completely dissolved.Then it is dispensed into 96 well culture plates, 3 parallel holes are made in each hole, and light is determined with 570nm wavelength with ELIASA
Density value.Also lysate can be directly moved into 2ml cuvettes, OD values is determined in wavelength 570nm on 721 spectrophotometers.
4th, result judgement
The propagation energy of lymphocyte is represented with adding the OD value of ConA fluid apertures to subtract to be not added with the OD value of ConA fluid apertures
Power, the optical density difference of test sample group are significantly higher than the optical density difference of control group, can determine that this experimental result positive.
5th, result of the test
Each test group optical density difference (with plus the OD value of ConA fluid apertures subtract and be not added with the OD value of ConA fluid apertures,
Take the difference average value of 10 mouse of each group) it is as shown in table 1 below：
Each test group optical density difference of table 1
| Packet | n | Optical density difference |
| Blank control group | 10 | 0.164±0.033 |
| Comparative example group | 10 | 0.566±0.221* |
| 1 group of embodiment | 10 | 0.772±0.289** |
| 2 groups of embodiment | 10 | 0.834±0.336** |
Note：Compared with blank control group：*P≤0.05；**P≤0.01.
From table 1 it follows that compared with blank control group, remaining each group optical density difference substantially increases, and prompts each group
The lymphopoiesis ability of mouse has strengthened.Comparatively, 2 groups of embodiment 1 and embodiment increase to mouse lymphocyte
Comparative example group will be substantially better than by growing the improvement of ability, illustrate composite glossy ganoderma extraction composition formula enhancing mouse immunity of the present invention
Effect be better than the product only made of ganoderma lucidum and rainbow conk compatibility.
(2) tumour functional evaluation is suppressed
1st, subjects and packet
Experimental animal：BALB/c mouse, 18-22g, male and female half and half, 40.Four groups are randomly divided into, 1 group of embodiment, is implemented
2 groups of example, comparative example group and blank control group, every group of 10 mouse.
2nd, modeling method
Murine sarcoma model is made using methyl cholanthrene legal system.
3rd, test method
The feeding method of animal：Modeling starts to start within first 3 days to give trial drug, and daily every of test group gavages compound spirit
Sesame extract (embodiment 1, embodiment 2 and comparative example product) 0.5ml (containing active ingredient 40mg)；Blank control group gavages consubstantiality
Ponding, continuous feeding is until one week after the completion of modeling.
4th, evaluation index
Off-test, observe each group Tumor incidence and knurl weight.
5th, result of the test
Each group mouse tumor incidence and knurl weight result are as shown in table 2 below：
The each group mouse tumor incidence of table 2 and knurl weight result
| Group | n | Tumor incidence (%) | Average knurl weight (g) |
| Blank control group | 10 | 100 | 0.392 |
| Comparative example group | 10 | 60 | 0.201 |
| 1 group of embodiment | 10 | 50 | 0.157 |
| 2 groups of embodiment | 10 | 40 | 0.169 |
From Table 2, it can be seen that after taking composite glossy ganoderma extract of the present invention, the Tumor incidence of mouse and average knurl weight
Significantly reduce, show that composite glossy ganoderma extract of the present invention has obvious suppression tumor function.
The preferred embodiments of the disclosure and embodiment are explained in detail above, but the present invention is not limited to
The above-described embodiment and examples, in those skilled in the art's possessed knowledge, the present invention can also not departed from
Various changes can be made on the premise of design.
Claims (10)
1. a kind of reinforced immunological and the composite glossy ganoderma extract for suppressing tumour, it is characterised in that it is the raw material by following weight parts
It is manufactured：
Ganoderma lucidum 10-15；Purple sesame 6-12；Red sesame 6-9；
The brown small pore fungi 6-9 of sesame；Yellow sesame 6-9；Rainbow conk 4-8.
2. reinforced immunological as claimed in claim 1 and the composite glossy ganoderma extract for suppressing tumour, wherein the parts by weight of each raw material are：
Ganoderma lucidum 10；Purple sesame 6；Red sesame 6；
The brown small pore fungi 6 of sesame；Yellow sesame 6；Rainbow conk 4.
3. reinforced immunological as claimed in claim 1 and the composite glossy ganoderma extract for suppressing tumour, wherein the parts by weight of each raw material are：
Ganoderma lucidum 12；Purple sesame 8；Red sesame 8；
The brown small pore fungi 8 of sesame；Yellow sesame 8；Rainbow conk 6.
4. reinforced immunological as claimed in claim 1 and the composite glossy ganoderma extract for suppressing tumour, wherein the parts by weight of each raw material are：
Ganoderma lucidum 15；Purple sesame 12；Red sesame 9；
The brown small pore fungi 9 of sesame；Yellow sesame 9；Rainbow conk 8.
5. the preparation method of the composite glossy ganoderma extract of reinforced immunological and suppression tumour as described in claim any one of 1-4,
It is characterized in that comprise the following steps：
(1) 8-10 times will be added after the mixing of ganoderma lucidum, purple sesame, red sesame, the small pore fungi of brown sesame and Huang Zhi medicinal materials, water rinsing, dry, crushing to measure
30% ethanol solution, after soaking 2 hours, refluxing extraction 1 hour, decoction filtration, filtrate is standby, under the same conditions by dregs of a decoction weight
Extract 1 time again, filtering, merging filtrate, 10 times of amount ethanol are added into gained filter residue：Ethyl acetate=1：3 mixed solutions, backflow
Extraction 1 hour, filtering, gained filtrate merge with foregoing filtrate, are spray-dried after being concentrated under reduced pressure into small size, must spray dry
Dry powder；
(2) ultramicro grinding will be carried out after the rinsing of rainbow conk medicinal material water, drying, gained ultra-micro powder obtains cloud after crossing 300-500 mesh sieves
Sesame Ultramicro-powder；
(3) above-mentioned spray dried powder and rainbow conk Ultramicro-powder are mixed, fully mixed in mixer, 20-40 mesh sieves are crossed after granulation,
Dry, whole grain, composite glossy ganoderma extract is produced after sterilization.
6. the preparation method of reinforced immunological as claimed in claim 5 and the composite glossy ganoderma extract of suppression tumour, its feature exist
In obtained composite glossy ganoderma extract by the conventional method for preparing capsule, tablet or granule in this area, further make
Obtain the agent of composite glossy ganoderma extract capsule, tablet or granule.
7. reinforced immunological as described in claim any one of 1-4 and suppress the composite glossy ganoderma extract of tumour prepare it is antitumor
Application in medicine.
8. reinforced immunological as described in claim any one of 1-4 and suppress the composite glossy ganoderma extract of tumour prepare it is antitumor
Application in health products.
9. the composite glossy ganoderma extract of the reinforced immunological and suppression tumour as described in claim any one of 1-4 is exempted from preparation raising
Application in epidemic disease power medicine.
10. the composite glossy ganoderma extract of the reinforced immunological and suppression tumour as described in claim any one of 1-4 is preparing raising
Application in immunity health products.
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| CN113413424A (en) * | 2020-09-07 | 2021-09-21 | 深圳市桦健量子生物科技有限公司 | Preparation method and application of lucid ganoderma oral liquid |
| CN113413424B (en) * | 2020-09-07 | 2022-05-27 | 深圳市桦健量子生物科技有限公司 | Preparation method and application of lucid ganoderma oral liquid |
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Application publication date: 20171219 |