CN107468858B - A kind of Chinese medicinal ointment and preparation method thereof for treating stagnation of QI due to depression of the liver psoriasis pustulosa - Google Patents

Abstract

The invention belongs to the field of medicine, in particular to a traditional Chinese medicinal ointment for treating psoriasis of liver depression and qi stagnation type. The traditional Chinese medicinal material prescription of the pharmaceutical composition is calculated according to the weight ratio: 27-36 parts by weight of fennel, 16-20 parts by weight of southern crane louse, 11-15 parts by weight of ground beetle, 9-12 parts by weight of borneol, It is prepared by 1-4 parts by weight of wind, 1-4 parts by weight of sorrel and 1-4 parts by weight of drunken vine. The traditional Chinese medicine ointment for treating psoriasis of liver depression and qi stagnation type is extracted by ethanol, concentrated into extractum, mixed with oil phase auxiliary materials and water phase auxiliary materials of the ointment, and emulsified.

Description

A traditional Chinese medicine ointment for treating psoriasis of liver depression and qi stagnation type and preparation method thereof

technical field

The invention belongs to the field of medicine, and in particular relates to a traditional Chinese medicinal ointment for treating psoriasis of liver depression and qi stagnation type and a preparation method thereof.

Background technique

Psoriasis is a common chronic relapsing skin disease. According to clinical features, it can be divided into vulgar, joint, pustular, and erythrodermic types. Among them, psoriasis vulgaris is the most common, accounting for more than 90%. Psoriatic lesions are red papules or erythema covered with thick silvery-white scales, with the characteristics of "film phenomenon, wax drop phenomenon, and spotting"; they mostly occur in the whole body, and are symmetrically distributed on the scalp, extensor sides of limbs, and sacrum , often accompanied by varying degrees of itching.

The pathogenesis of psoriasis has not been studied clearly in modern medicine. Current studies suggest that the disease is related to multiple factors such as genetics and environment, and is related to immune-mediated keratinocyte proliferation. Chemotherapy mostly uses immunosuppressive drugs, adrenal cortex hormones, antibiotics, etc. Because of its recurrent attacks, the side effects of chemical drugs are relatively large and the effect is not ideal, which limits its clinical application.

Psoriasis is also called " white scar ", " psoriasis ", " pine skin tinea ", " white shell sore ", " snake lice " etc. in Chinese medicine literature. Through long-term clinical practice and exploration, Chinese medicine has formed some systematic theories and methods in the treatment of psoriasis, and has achieved certain curative effects. In terms of TCM treatment, the syndrome types of psoriasis are mainly blood-heat type, blood stasis type, blood-dryness type, and blood-deficiency type, and other syndrome types such as damp-heat type, wind-heat type, wind-cold type, liver-kidney deficiency type, Qi stagnation type, spleen and kidney deficiency type, and Chong-ren imbalance syndrome type are less. Therefore, on the basis of syndrome differentiation and treatment, the current treatment of psoriasis mainly adopts blood-enriching, blood-cooling and blood-activating drugs. The most commonly used ones include rehmannia root, angelica, paeonol, licorice, red peony root, salvia miltiorrhiza, white fresh skin, smilax cocos, and comfrey. Other drugs are mainly heat-clearing and detoxifying drugs, blood-activating and stasis-removing drugs, and deficiency-reinforcing drugs. For the compatibility of medicine pairs, Shengdi + Cortex Moutan, Angelica + Chuanxiong, and Rehmannia + Angelica are used most frequently, forming a basic treatment principle characterized by clearing away heat and detoxification, promoting blood circulation and removing blood stasis, and tonifying deficiency. For psoriasis of liver depression and qi stagnation type, there is no ideal treatment prescription at present.

Therefore, it is still necessary to screen and study the efficacy of traditional Chinese medicine prescriptions for psoriasis with liver depression and qi stagnation, so as to provide treatment options for clinical treatment.

Contents of the invention

For above-mentioned prior art, one of purpose of the present invention is to provide a kind of Chinese medicinal ointment for the treatment of psoriasis of stagnation of liver-QI stagnation type, for realizing above-mentioned purpose, the technical scheme that the present invention adopts is as follows:

A traditional Chinese medicine ointment for treating psoriasis of liver depression and qi stagnation type, which is made of Chinese medicinal materials in the following weight ratios as raw materials

27-36 parts by weight, 16-20 parts by weight of southern crane lice, 11-15 parts by weight of ground beetle, 9-12 parts by weight of borneol, 1-4 parts by weight of earth-boring wind, 1-4 parts by weight of sorrel, drunk soul Rattan 1-4 parts by weight.

In a preferred technical solution, the traditional Chinese medicine ointment is made of the following Chinese medicinal materials in parts by weight as raw materials: 31 parts by weight of Rhododendron chinensis, 17 parts by weight of Southern crane lice, 14 parts by weight of ground beetle, 10 parts by weight of borneol, 3 parts by weight of ground wind, 2 parts by weight of sorrel, and 2 parts by weight of drunken vine.

In another preferred technical solution, the traditional Chinese medicine ointment is made of the following Chinese medicinal materials in parts by weight as raw materials: 35 parts by weight of Rhododendron chinensis, 19 parts by weight of southern crane louse, 12 parts by weight of wood beetle, 11 parts by weight of borneol, 2 parts by weight of earth-boring wind, 3 parts by weight of wood sorrel, and 3 parts by weight of drunken vine.

In another preferred technical solution, the traditional Chinese medicine ointment is made of the following Chinese medicinal materials in the following weight ratios as raw materials: 28 parts by weight of fennel, 18 parts by weight of southern crane louse, 13 parts by weight of ground beetle, 11 parts by weight of borneol, 3 parts by weight of ground wind, 3 parts by weight of sorrel, and 3 parts by weight of drunken vine.

Preferably, the above-mentioned traditional Chinese medicine ointment is one of ointment and cream.

Preferably, the above-mentioned traditional Chinese medicine ointment also contains pharmaceutically acceptable pharmaceutical excipients.

Preferably, the pharmaceutically acceptable excipients include stearic acid, glycerol monostearate, liquid paraffin, petrolatum, lanolin, triethanolamine, ethylparaben, azone and deionized water.

Another aspect of the present invention provides the preparation method of the above-mentioned traditional Chinese medicine ointment for the treatment of liver depression and qi stagnation type psoriasis, comprising the following steps:

1) Take the prescribed amount of fenugreek, southern crane lice, ground beetle, earth-burrowing wind, wood sorrel, and drunken vine, and crush them separately, and pass through a 70-mesh sieve.

Mixed medicinal powder;

2) According to the ratio of solid-liquid ratio of 1:3 to 1:6, add the concentration of 60% to 70% to the medicinal powder obtained in step 1).

Soak in ethanol for 2 to 4 hours, then reflux extraction in a constant temperature water bath at 70°C for 4 hours, let it cool and filter, and obtain the alcoholic extract of the medicinal material;

3) Evaporate and concentrate the alcoholic extract of medicinal materials obtained in step 2) to a relative density of 1.15-1.35, add the prescribed amount of

Borneol, stirred evenly to obtain medicinal material extract;

4) Mix the extract obtained in step 3) with pharmaceutically acceptable auxiliary materials to prepare a traditional Chinese medicine ointment.

Preferably, the solid-to-liquid ratio in step 2) is 1:4, the ethanol concentration is 65%, and the soaking time in ethanol is 3.5 hours; the alcohol extract of medicinal materials in step 3) is evaporated and concentrated to a relative density of 1.32.

Preferably, the above step 4) includes the following steps:

a, get the stearic acid of medicinal material extract weight 50%, the glyceryl monostearate of medicinal material extract weight 12%, the liquid paraffin of medicinal material extract weight 20%, the vaseline of medicinal material extract weight 8%, medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

B, get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to dissolving completely, as water Mutually;

c. Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Dioscorea hypoglauca Palibin described in the technical solution of the present invention is the dry rhizome of Dioscorea hypoglauca Palibin. Bitter, flat. Return kidney, stomach warp. Efficacy is dampness removing turbidity, dispelling wind and removing numbness. Its source and medicinal material standards are included in the Chinese Pharmacopoeia 2015 edition.

The southern crane lice described in the technical solution of the present invention is the dry and mature fruit of the wild carrot Daucuscarota L. of the Umbelliferae plant. Bitter, pungent, flat; slightly poisonous. Return spleen, stomach warp. The effect is to kill insects and eliminate accumulation. Its source and medicinal material standards are included in the Chinese Pharmacopoeia 2015 edition.

The ground beetle described in the technical solution of the present invention is the dried female body of Eupolyphaga Sinesis Walker or Steleophaga plancyi (Boleny), an insect of the cockroach family Eupolyphaga Sinesis Walker. Salty, cold; slightly poisonous. Return liver channel. Efficacy is breaking blood and removing blood stasis, renewing tendons and bones. Its source and medicinal material standards are included in the Chinese Pharmacopoeia 2015 edition.

The ground-boring wind described in the technical solution of the present invention is the root and stem of the saxifrage plant Schizophragmaintegrifolium (Franch.) Oliv. Cool in nature, mild in taste, enters the spleen meridian. The effect is to dispel wind and dampness, and its source and properties are included in the "Dictionary of Chinese Medicine".

The wood sorrel described in the technical solution of the present invention is the dry whole grass of Oxalis corniculata Linnaeus of Oxalis family plant. sour, cold. Go into liver, lung, urinary bladder channel. Efficacy is clearing heat and dampness, cooling blood and dissipating blood stasis, reducing swelling and detoxification.

The drunken vine described in the technical solution of the present invention is the root of Heterostemma alatum Wight, bitter; flat. Go into liver, kidney channel. The effect is to dispel stagnation and eliminate dampness; to ward off filth and treat malaria.

The inventors of the present invention have found through a large number of prescription screenings that the external application of the composition of fenugreek, southern crane louse, wood beetle, and borneol has poor therapeutic effect on psoriasis of liver depression and qi stagnation type. There is no significant therapeutic effect on psoriasis of stagnation of liver and qi stagnation, but when the above-mentioned traditional Chinese medicine is combined in a specific proportion and used in combination with Drunken Soul Vine in a specific proportion, the external application has an unexpected therapeutic effect on psoriasis of stagnation of liver and qi stagnation , and the cost is lower, and the efficacy of the drug is exerted faster.

Detailed ways

Below in conjunction with embodiment the present invention is further explained. It should be understood that the following examples are only used to explain the present invention, rather than limit the protection scope of the present invention.

Example 1 Traditional Chinese Medicine Ointment for Treating Liver Qi Stagnation Type Psoriasis and Its Preparation

The prescription of traditional Chinese medicine ointment for treating psoriasis of stagnation of liver stagnation and qi stagnation is shown in the table below:

Preparation:

1) Take the prescribed amount of fenugreek, southern crane lice, ground beetle, earth-burrowing wind, wood sorrel, and drunken vine, and crush them separately, and pass through a 70-mesh sieve.

Mixed medicinal powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32, add the prescribed amount of borneol after cooling,

Stir evenly to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Example 2 Chinese medicine ointment for treating psoriasis of stagnation of liver stagnation and qi stagnation and its preparation

The prescription of traditional Chinese medicine ointment for treating psoriasis of stagnation of liver stagnation and qi stagnation is shown in the table below:

Preparation:

1) Take the prescribed amount of fenugreek, southern crane lice, ground beetle, earth-burrowing wind, wood sorrel, and drunken vine, and crush them separately, and pass through a 70-mesh sieve.

Mixed medicinal powder;

2) Add 70% ethanol to the medicinal material powder obtained in step 1) according to the ratio of solid to liquid: 1:5 for soaking

3.0h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.35, add the prescribed amount of borneol after cooling,

Stir evenly to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Example 3 Preclinical Pharmacodynamics Study of Traditional Chinese Medicine Ointment for Treatment of Liver Qi Stagnation Type Psoriasis

Medicines and their preparation: The traditional Chinese medicine ointments prepared by prescription 3, prescription 4, and prescription 5 of Example 1 were used in the experiment as experimental medicines; in order to compare the effects of medicines, the following 6 kinds of comparative medicines were specially prepared as positive controls:

Comparative example 1: The formula ratio is 360 g of fennel, 200 g of southern crane louse, 150 g of ground beetle, and 120 g of borneol. The preparation method is as follows:

1) Take the prescribed amount of fennel, southern crane lice, and ground beetle, grind them separately, pass through a 70-mesh sieve, and mix to obtain medicinal powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32, add the prescribed amount of borneol after cooling,

Stir evenly to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Comparative example 2: The prescription ratio is 400g of ground-boring wind and 400g of sorrel. The preparation method is as follows:

1) Take the prescription amount of the ground wind and wood sorrel, grind them separately, pass through a 70-mesh sieve, and mix to obtain medicinal powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32 to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Comparative example 3: The prescription ratio is 800g of Drunken Soul Vine, and the preparation method is as follows:

1) Take the prescription amount of Drunken Soul Vine, crush it, and pass it through a 70-mesh sieve to obtain medicinal powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32 to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Comparative example 4: The formula ratio is 360g of fenugreek, 200g of southern crane lice, 150g of ground beetle, 120g of borneol, 40g of earth-boring wind, and 40g of sorrel. The preparation method is as follows:

1) Take the prescribed amount of fennel, southern crane lice, ground beetle, ground borer, and wood sorrel. Pulverize respectively, cross 70 mesh sieves, and mix to obtain medicinal material powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32, add the prescribed amount of borneol after cooling,

Stir evenly to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Comparative example 5: The formula ratio is 360g of fennel, 200g of southern crane louse, 150g of ground beetle, 120g of borneol, and 40g of drunken vine. The preparation method is as follows:

1) Take the prescribed amount of fenugreek, southern crane lice, ground beetle, and drunken vine. Pulverize respectively, cross 70 mesh sieves, and mix to obtain medicinal material powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32, add the prescribed amount of borneol after cooling,

Stir evenly to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Comparative example 6: The prescription ratio is 400g of ground-boring wind, 400g of sorrel, and 400g of drunken vine. The preparation method is as follows:

1) Take the prescription amount of ground wind, wood sorrel, and drunken vine, grind them separately, pass through a 70-mesh sieve, and mix to obtain medicinal powder;

2) According to the ratio of solid to liquid of 1:4, add 65% ethanol to the medicinal material powder obtained in step 1) to soak

3.5h, then reflux extraction in a constant temperature water bath at 70°C for 4h, let it cool and filter to obtain the alcoholic extract of medicinal materials;

3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.32 to obtain medicinal material extract;

4) Take stearic acid with 50% weight of medicinal material extract, glyceryl monostearate with 12% weight of medicinal material extract, liquid paraffin with 20% weight of medicinal material extract, vaseline with 8% weight of medicinal material extract, and medicinal material extract 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use;

Get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to completely dissolving, as water phase;

Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.

Animal model and group administration: SPF-grade male BALB/c mice, weighing 18-20 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd., and were raised in an SPF-grade animal room. Adaptive feeding for 1 week before the experiment. Mice were anesthetized by intraperitoneal injection of pentobarbital sodium 80 mg/kg. The back hair of each mouse was shaved to form an exposed area with an area of 2 cm×3 cm. Apply 5% imiquimod cream 200mg/kg on the exposed area of the back of the mouse every day, and apply it continuously for 14 days to build the model; during the model making process, the mice were kept in single cages, and the mice were fixed in stainless steel mice every night The instrument (Beijing Heli Science and Technology Development Co., Ltd.) was restrained overnight. The skin lesions, erythema, scales, and infiltration in the exposed area were regarded as successful modeling. 60 mice successfully modeled were randomly selected and divided into 10 groups with 6 mice in each group.

Group 1 was the negative control group, and normal saline was applied to the skin of the modeling area once a day for a total of 14 days;

The 2nd to 4th groups are the experimental group, apply the traditional Chinese medicine ointment prepared by the formula 3, the formula 4 and the formula 5 of the embodiment 1 respectively on the skin of the modeling area, and the dosage is calculated as 3g/piece/day based on the medicinal materials of the powdered scorpion. , a total of 14 days.

Groups 5-7 belonged to the positive control group, and the traditional Chinese medicine ointments prepared in Comparative Examples 1, 4, and 5 were applied to the skin of the modeling area, and the dosage was calculated as 3 g/piece/day based on the raw medicinal materials of fennel, for a total of 14 days.

Groups 8 and 9 belonged to the positive control group. The traditional Chinese medicine ointments prepared in Comparative Examples 2 and 6 were applied to the skin of the modeling area, and the dosage was calculated as 0.45 g/piece/day based on the medicinal materials of the earth-burrowing wind, for a total of 14 days.

Group 10 belonged to the positive control group. The traditional Chinese medicine ointment prepared in Comparative Example 3 was applied to the skin of the modeling area, and the dosage was calculated as 0.45 g/piece/day based on the medicinal material of Drunken Vine, for a total of 14 days.

Investigation indicators: From the day of the first administration to the 7th day after the end of the administration, the skin of the mouse modeling area was observed by digital photography every day. Observe the erythema, infiltration and desquamation of the skin lesion area, evaluate the psoriasis severity of rats in each group according to the psoriasis lesion area and severity score (PASI score), and divide the three items of erythema, infiltration and desquamation according to the severity. Indexes are assigned 0-4 points (0 asymptomatic, 1 mild, 2 moderate, 3 severe, 4 extremely severe), and the total PASI score (0-12 points) is obtained by adding the three points. Animals in each group were compared by calculating the mean and standard deviation based on the total PASI integral of each animal. The t test was used for comparison between the two groups. P<0.05 was regarded as significant difference, and p<0.01 was regarded as extremely significant difference.

Results: On the 7th day after the end of the administration, the total PASI score results of the erythema, infiltration, and desquamation degree of the mice in each group are shown in the table below:

Compared with negative control group a: p<0.05, b: p<0.01; compared with comparative example 1 positive control group c: p<0.05, d: p<0.01.

It can be seen from the above table that, under the condition of the same dosage of fenugreek, after 14 days of administration of prescriptions 3, 4, and 5, the total PASI score of the mice was extremely significantly lower than that of the negative control group (p<0.01), and the comparative example 1 was positive. The total PASI score of the control group was significantly lower than that of the negative control group (p<0.05); the total PASI score of prescriptions 3, 4, and 5 after administration was significantly lower than that of the positive control group of comparative example 1 (p<0.01). It shows that prescriptions 3, 4, and 5 can improve the symptoms of psoriasis mice with liver depression and qi stagnation type significantly better than the traditional Chinese medicine ointment of comparative example 1.

There was no significant difference in the total PASI score of mice in the positive control group of Comparative Example 2, the positive control group of Comparative Example 3 and the positive control group of Comparative Example 6 and the negative control group (p>0.05), indicating that Comparative Example 2 (earth-boring wind, wood sorrel Recipe), Comparative Example 3 (Zuihunteng prescription), Comparative Example 6 (Zidifeng, Sorrel, Zuixunteng prescription) improved the symptoms of psoriasis mice with liver depression and qi stagnation The effect was not significantly different from that of normal saline.

The total PASI score of mice in the positive control group of Comparative Example 4 and the positive control group of Comparative Example 5 was significantly lower than that of the negative control group (p<0.05), but there was no significant difference compared with the positive control group of Comparative Example 1 (p>0.05), indicating that There was no significant synergistic effect of the two Chinese medicines of Zundifeng and Sorrel or the single Chinese medicine of Zuixunteng on the prescriptions of Fleuria spp.

In summary, the combined use of the three traditional Chinese medicines of burrowing wind, wood sorrel, and drunken vine has a significant synergistic effect on the prescriptions of A. The symptoms of skin lesions can be significantly improved.

In addition, it should be understood that although this specification is described according to implementation modes, not each implementation mode only includes an independent technical solution, and the narration method of the specification is only for clarity, and those skilled in the art should take the specification as a whole, and each The technical solutions in the embodiments can also be properly combined to form other implementations that can be understood by those skilled in the art.

Claims (10)

1. A Chinese medicine ointment for the treatment of psoriasis of liver depression and qi stagnation type, characterized in that, it is made from the following Chinese medicinal materials in parts by weight: 27 to 36 parts by weight of fennel, 16 to 20 parts by weight of licorice , 11-15 parts by weight of wood beetle, 9-12 parts by weight of borneol, 1-4 parts by weight of earth-boring wind, 1-4 parts by weight of sorrel, and 1-4 parts by weight of drunken vine. 2. the Chinese medicine ointment for the treatment of liver stagnation and stagnation of qi stagnation type psoriasis according to claim 1, is characterized in that, described Chinese medicine ointment is made as raw material by the Chinese medicinal material of following ratio by weight: Fenugreek 31 weight parts, south 17 parts by weight of crane lice, 14 parts by weight of ground beetle, 10 parts by weight of borneol, 3 parts by weight of earth-boring wind, 2 parts by weight of sorrel, and 2 parts by weight of drunken vine. 3. the Chinese medicinal ointment for the treatment of liver depression and qi stagnation type psoriasis according to claim 1, is characterized in that, described Chinese medicinal ointment is made as raw material by the Chinese medicinal material of following ratio by weight: Fenugreek 35 weight parts, southern 19 parts by weight of crane lice, 12 parts by weight of ground beetle, 11 parts by weight of borneol, 2 parts by weight of earth-boring wind, 3 parts by weight of sorrel, and 3 parts by weight of vine. 4. the Chinese medicine ointment for the treatment of liver depression and qi stagnation type psoriasis according to claim 1, is characterized in that, described Chinese medicine ointment is made as raw material by the Chinese medicinal material of following ratio by weight: Fenugreek 28 weight parts, south 18 parts by weight of crane lice, 13 parts by weight of ground beetle, 11 parts by weight of borneol, 3 parts by weight of earth-boring wind, 3 parts by weight of sorrel, and 3 parts by weight of drunken vine. 5. The Chinese medicine ointment for treating liver-qi stagnation type psoriasis according to any one of claims 1 to 4, characterized in that, said Chinese medicine ointment is one of ointment and cream. 6. The traditional Chinese medicine ointment for treating liver depression and qi stagnation type psoriasis according to claim 5, characterized in that, said Chinese medicine ointment also contains pharmaceutically acceptable pharmaceutical excipients. 7. the Chinese medicinal ointment for the treatment of liver depression and qi stagnation type psoriasis according to claim 6, is characterized in that, described pharmaceutically acceptable pharmaceutical adjuvant comprises stearic acid, glyceryl monostearate, liquid Paraffin, Petrolatum, Lanolin, Triethanolamine, Ethyl Paraben, Azone, and Deionized Water. 8. The preparation method of the Chinese medicinal ointment for the treatment of liver-qi stagnation type psoriasis described in any one of claims 1 to 4, is characterized in that comprising the following steps: 1) Take the prescription amount of fennel, southern crane lice, ground beetle, earth-boring wind, wood sorrel, and drunken vine, and grind them separately, pass through a 70-mesh sieve, and mix to obtain medicinal powder; 2) Add 60% to 70% ethanol to the medicinal material powder obtained in step 1) according to the ratio of solid to liquid 1:3 to 1:6, soak for 2 to 4 hours, and then extract in a constant temperature water bath for 4 hours at 70°C. Let it cool and filter to obtain the medicinal material alcoholic extract; 3) Evaporate and concentrate the medicinal material alcohol extract obtained in step 2) to a relative density of 1.15-1.35, add the prescribed amount of borneol after cooling, and stir evenly to obtain the medicinal material extract; 4) Mix the extract obtained in step 3) with pharmaceutically acceptable auxiliary materials to prepare a traditional Chinese medicine ointment. 9. The preparation method of traditional Chinese medicine plaster for treating liver stagnation and qi stagnation type psoriasis according to claim 8, characterized in that, the solid-liquid ratio in step 2) is 1:4, the ethanol concentration is 65%, and the ethanol The soaking time is 3.5 hours; the alcoholic extract of medicinal materials described in step 3) is evaporated and concentrated to a relative density of 1.32. 10. The preparation method of traditional Chinese medicine ointment for treating psoriasis of liver stagnation and qi stagnation type according to claim 8 or 9, characterized in that, the step 4) comprises the following steps: a, get the stearic acid of medicinal material extract weight 50%, the glyceryl monostearate of medicinal material extract weight 12%, the liquid paraffin of medicinal material extract weight 20%, the vaseline of medicinal material extract weight 8%, medicinal material extractum 20% by weight of lanolin and 4% by weight of medicinal extracts of azone are placed in a container, heated and melted in a water bath, stirred evenly, used as an oil phase, and kept at a constant temperature of 70°C for later use; B, get the triethanolamine of medicinal material extract weight 2.8%, the paraben ethyl ester of medicinal material extract weight 2% and the deionized water of medicinal material extract weight 191% are placed in another container, be heated to dissolving completely, as water Mutually; c. Slowly pour the water phase into the oil phase, stir evenly, add the medicinal material extract obtained in step 3), stir while adding, until the emulsification is complete, let it cool, and then fill it to obtain an ointment.