CN107432193A - A kind of method of fast breeding winter rape seedling - Google Patents
A kind of method of fast breeding winter rape seedling Download PDFInfo
- Publication number
- CN107432193A CN107432193A CN201710905549.9A CN201710905549A CN107432193A CN 107432193 A CN107432193 A CN 107432193A CN 201710905549 A CN201710905549 A CN 201710905549A CN 107432193 A CN107432193 A CN 107432193A
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- China
- Prior art keywords
- seedling
- seed
- rape
- fast breeding
- winter rape
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a kind of fast breeding winter rape seedling and the method for improving cold-hartliness, comprise the following steps：Seed-soaking liquid is prepared, hydrogen peroxide (H2O2) seed soaking, germinating bed preparation, incubator culture, transplanting.This method is by combining low concentration H2O2Hole plate seedling growth under seed-soaking liquid seed soaking and greenhouse, rape nursery stage is not only significantly reduced, also advantageously improves the vigor and seedling cold-hartliness of seed, it is nervous so as to alleviate the rape land used caused by the preceding crop ripe phase postpones, and seedling stage cold wave freeze injury problem.Simultaneously this method also can to because environment is unfavorable or mismanagement caused by weak seedling, the situation of being short of seedling remedy, the cold resistance that the later stage mends planting rape seedling is improved, so as to keep the stable yields not underproduction of overall yield.The inventive method is easy reliable, cost is cheap, can be applicable on a large scale, while shortening rape seedling raise period early stage, production loss caused by saving because of weak seedling freeze injury or being short of seedling in time.
Description
Technical field
The present invention relates to a kind of culture of plant, more particularly to a kind of method for breeding winter rape seedling.
Background technology
In Middle And Lower Reaches of Changjiang River is an important producing region of China's rape, once accounts for the near of the total cultivated area of national rape
70%, Rape-seed production cropping pattern mainly uses rice field-upland field rotation.In recent years due to the postponement of rice ripe phase, rape land used day is caused
It is becoming tight, rape date of seeding is also delayed therewith.Under the overall situation of global warming, extreme weather is increasing, rape fertility
Interim cold wave generation is increasingly frequent, so as to cause the significantly decline of yield of rape, influences plantation of the peasant to rape and it is expected.But
With the high speed development of industry, the problem of grease supply in the world's has tended to be nervous, and China equally faces oil supply amount deficiency.
How to solve the problems, such as current rape land used anxiety, seedling stage freeze injury is pendulum pressing problem before eyes.
The upper rapeseed cultivation mode of production at present is mainly transplanted and live, and transplanted seedling is typically sowed in mid or late September, live
Seedling is typically sowed in early October, the postponement of rice ripe phase and the unbroken wet weather in sowing time, causes the delay of rape seed phase,
Later stage meets low temperature and causes slow-growing, and increment diminishes, so as to influence solid and ultimate output.Therefore fast breeding winter rape children
Seedling, while improve seedling stage cold-hartliness and just seem very necessity.
The content of the invention
Goal of the invention：The present invention provides a kind of method of fast breeding winter rape seedling, solves in Brassica campestris L seedling cultivating process
It is slow to send out seedling, frigolabile problem.
Technical scheme：A kind of method of fast breeding winter rape seedling of the present invention, is comprised the steps of：(1) soak seed
The configuration of liquid：Configuration quality percentage concentration is 0.01-0.1% H2O2Solution；(2) soak seed：Winter rape seed is immersed into seed-soaking liquid
Immersion, pour out and seed cleaned after seed-soaking liquid, dried；(3) prepare germinating bed, matrix is put into germinating bed, will be obtained in step (2)
To rape seed be seeded into germinating bed；(4) rape seed after planting is watered, is put into incubator culture；(5) to step
(4) seedling of culture carries out cold-resistant processing, transplants seedling.
In step (1), the H2O2Solution quality percentage concentration is 0.04-0.06%.Most preferably, H2O2Solution quality
Percentage concentration is 0.05%.
In step (2), the seed soaking time is 9-11h, and most preferably, the time of seed soaking is 10h.Time passes short effect not
Significantly, overlong time (being more than 24h) can cause kind of a skin spalling, influence germination percentage, lopsided seedling easily occurs in the later stage.
In step (3), the germinating bed is black hole tray；The hole tray specification is 200 holes or 288 holes, stock size are
54cm×28cm。
It is 1 that the matrix, which includes mass ratio,:1:1 black wood charcoal soil, vermiculite and perlite.Matrix require it is sterile, without worm's ovum, nothing
Impurity, there are good water-retaining property and gas permeability, when germinating bed is hole tray, all fill matrix in every cave, and each cell compartment clearly may be used
See and be defined, hole tray management is the same as general production management.Before sowing, vertically it is placed in the hole tray for filling matrix with identical hole disk,
Two hands lie on the disk of hole and gently pushed, and ensure that the sowing depth is consistent, emergence is neat, by seed point in the disk in cave is pressed,
Put 3 in each hole central point, after planting cover former base matter, scrape other end from one of disk with scraper plate, make matrix face with
Card is equal.It is permeable (avoid flood to pour, in order to avoid seed is gone out into hole tray) with the spray of the watering can of fine-meshed shower nozzle after hole tray is set,
It is then placed in illumination box and (can be put into during scale in greenhouse), cultivation temperature is 24-26 DEG C, and optimal cultivation temperature is
25 DEG C of seedling managements are the same as general production measure.
In step (5), the seedling is in the 6-8 leaf phases.The cold-resistant processing is 4-6 days before transplanting, and cultivation temperature is daily
Reduce 2-3 DEG C.
Unless stated otherwise, " % " is mass percent in the present invention.
Beneficial effect：The present invention uses low concentration H2O2Hole plate seedling growth under seed-soaking liquid seed soaking and greenhouse, than conventional kind
Rape nursery stage under plant mode shortens 7 to 10 days；The present invention not only can suitably break rape seed rest period, improve seedling
Cold-hartliness, moreover it is possible to create optimum Germination Condition, it is bright under the more conventional planting patterns of emergence rate and cold-hartliness of seed
It is aobvious to improve；Be advantageous to alleviate that the land used caused by rice ripe phase postpones is nervous and seedling stage freeze injury problem, do not subtract while stable yields
Production.Method is easy easily to be grasped, with seed-soaking liquid H2O2It is easily obtained, prepares simple；Water and fertilizer management is the same as general raw during whole nursery
Production, is not required to additionally put into.
Embodiment
First, raw material prepares
1st, seed-soaking liquid：The 30% mass percentage concentration H sold with the market2O2(analysis is pure, the production of Shanghai Chinese medicines group) matches somebody with somebody
Put various concentrations H2O2Seed-soaking liquid, it is now with the current.
2nd, rape seed selects：In September, 2015 is some from the rape variety town oil 7 that field newly harvests then.
3rd, the configuration of matrix：It is 1 in mass ratio by black wood charcoal soil, vermiculite and perlite:1:1 mixing.
2nd, metrics evaluation standard
Endure cold seedling：Aggrieved with indivedual great Ye, aggrieved leaf part atrophy or presentation canescence freeze injury patch are as seedling mark of enduring cold
It is accurate.
3rd, seedling culture
Embodiment 1：Seed is put into the 0.05%H prepared2O2In seed-soaking liquid, do not had seed, it is small to soak 10 under indoor environment
When, seed-soaking liquid is poured out, clear water is dried after rinsing well repeatedly to wait to sow.200 hole black hole trays are chosen as germinating bed, per cave
In all fill matrix, and each cell compartment is high-visible is defined, and is vertically placed in the hole tray for filling matrix with identical hole disk, two
Hand lies on the disk of hole and gently pushed, and randomly selects full healthy seed to be sowed, by seed point in the disk in cave is pressed,
Put 3 in each hole central point, after planting cover former base matter, scrape other end from one of disk with scraper plate, make matrix face with
Card is equal, and hole tray management after hole tray is set, each uses the watering can spray of fine-meshed shower nozzle permeable, then with general production management
It is put into illumination box, temperature control is at 25 ± 1 DEG C, and seedling management is the same as general production measure.Germination percentage is counted after 7 days.When
Transplanted during seedling 6-8 leaves, 2 DEG C of progress low temperature environment adaptations of drop daily in 5 days before transplanting, will be taken out by the seedling of cold-resistant processing in
Normal water management, the unified number of days for recording 50% seedling and the 7th leaf occur are carried out under normal natural lighting.
Control 1：Hole plate seedling growth under natural environment, seed-soaking liquid processing is not carried out, not the hole plate seedling growth under greenhouse, remaining is the same as real
Apply example 1.
Control 2：Seed carries out seed-soaking liquid processing, not the hole plate seedling growth under greenhouse, and remaining is the same as embodiment 1.
Control 3：Seed-soaking liquid processing, but the hole plate seedling growth under greenhouse are not carried out, and remaining is the same as embodiment 1.
4th, handle
1st, embodiment 1, control 1, control 2 are uniformly put into 5 DEG C of illumination boxs at low temperature with compareing the seedling of 3 cultures
Reason 24 hours, take out normal water management growth 5 days is carried out under normal natural lighting afterwards, count seedling number of enduring cold.
2nd, the H of various concentrations is configured2O2Seed-soaking liquid, seed is handled with the seed-soaking liquid of various concentrations, specific method is same
Embodiment 1, Brassica Napus Seedling is cultivated, influence of the statistics seed-soaking liquid concentration to Brassica Napus Seedling, the results are shown in Table 2.
5th, result
There is the 7th leaf number of days, germination percentage, the statistical result for seedling of enduring cold in town oil 7 under the conditions of the different disposal of table 1
The various concentrations seed-soaking liquid of table 2 processing town oil seventh-seeded seedling stage cold-hartliness compares
From table 1, we can be found that hole plate seedling growth is with compareing hole plate seedling growth under 1 normal natural environment under 2 greenhouses of control
Compare, can suitably shorten rape nursery stage, but not as hole plate seedling growth shortens nursery under greenhouse after the place's seed-soaking liquid of embodiment 1 processing
Phase is obvious, while can not improve the cold-hartliness of Brassica Napus Seedling, reduces the cold-hartliness of seedling on the contrary.By comparing 4 kinds of different disposals,
We can be found that 0.05% concentration H2O2Seed-soaking liquid pretreatment seed is remarkably improved the cold-hartliness of seedling.
From table 2, we can be found that external source H2O2Solution can improve the cold-hartliness and germination percentage of seedling, but too high
Concentration (>3%) cold-hartliness and percentage of seedgermination of seedling can be influenceed.Suitable H2O2Seed-soaking liquid concentration is 0.01%-0.1%,
Bud ratio can not only be improved, moreover it is possible to seedling cold-hartliness is improved, wherein 0.05% concentration or so is optimal seed-soaking liquid concentration.
Claims (9)
- A kind of 1. method of fast breeding winter rape seedling, it is characterised in that comprise the steps of：(1) configuration of seed-soaking liquid：Configuration quality percentage concentration is 0.01-0.1% H2O2Solution；(2) soak seed：Winter rape seed is immersed into seed-soaking liquid immersion, is poured out and seed is cleaned after seed-soaking liquid, dried；(3) prepare germinating bed, matrix is put into germinating bed, the rape seed obtained in step (2) is seeded into germinating bed；(4) rape seed after planting is watered, is put into incubator culture；(5) cold-resistant processing is carried out to the seedling of step (4) culture, transplants seedling.
- 2. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (1), the H2O2 Solution quality percentage concentration is 0.04-0.06%.
- 3. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (1), the H2O2 Solution quality percentage concentration is 0.05%.
- 4. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (2), the leaching The kind time is 9-11h.
- 5. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (3), the hair Bud bed is black hole tray；The hole tray specification is 200 holes or 288 holes.
- 6. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (3), the base It is 1 that matter, which includes mass ratio,:1:1 black wood charcoal soil, vermiculite and perlite.
- 7. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (4), the training It is 24-26 DEG C to support temperature.
- 8. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that in step (5), the children Seedling is in the 6-8 leaf phases.
- 9. the method for fast breeding winter rape seedling according to claim 1, it is characterised in that described resistance in step (5) Cold processing is 4-6 days before transplanting, and cultivation temperature reduces 2-3 DEG C daily.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710905549.9A CN107432193A (en) | 2017-09-29 | 2017-09-29 | A kind of method of fast breeding winter rape seedling |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710905549.9A CN107432193A (en) | 2017-09-29 | 2017-09-29 | A kind of method of fast breeding winter rape seedling |
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| CN107432193A true CN107432193A (en) | 2017-12-05 |
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| CN201710905549.9A Pending CN107432193A (en) | 2017-09-29 | 2017-09-29 | A kind of method of fast breeding winter rape seedling |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366330A (en) * | 2008-09-01 | 2009-02-18 | 山东省农业科学院作物研究所 | Initiating agent for accelerating quick germination of wheat seeds and method of use thereof |
| CN102577816A (en) * | 2012-03-13 | 2012-07-18 | 湖南水木生物技术有限公司 | Slope crop seeding raising method |
| CN104012387A (en) * | 2014-01-21 | 2014-09-03 | 无锡思清源生物科技有限公司 | Organic composite substrate for oilseed rape tray-soilless seedling and preparation method thereof |
| CN104365349A (en) * | 2014-11-02 | 2015-02-25 | 隋丕向 | High-quality and high-efficiency cultivation technology for rape in open field |
| CN107155583A (en) * | 2017-05-27 | 2017-09-15 | 明光市裕阳农业有限公司 | A kind of breeding method of rape |
-
2017
- 2017-09-29 CN CN201710905549.9A patent/CN107432193A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101366330A (en) * | 2008-09-01 | 2009-02-18 | 山东省农业科学院作物研究所 | Initiating agent for accelerating quick germination of wheat seeds and method of use thereof |
| CN102577816A (en) * | 2012-03-13 | 2012-07-18 | 湖南水木生物技术有限公司 | Slope crop seeding raising method |
| CN104012387A (en) * | 2014-01-21 | 2014-09-03 | 无锡思清源生物科技有限公司 | Organic composite substrate for oilseed rape tray-soilless seedling and preparation method thereof |
| CN104365349A (en) * | 2014-11-02 | 2015-02-25 | 隋丕向 | High-quality and high-efficiency cultivation technology for rape in open field |
| CN107155583A (en) * | 2017-05-27 | 2017-09-15 | 明光市裕阳农业有限公司 | A kind of breeding method of rape |
Non-Patent Citations (1)
| Title |
|---|
| 万林: "H2O2浸种对直播油菓生长和抗寒性的影响", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
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Application publication date: 20171205 |