CN107428800A - 一种抑制ampa受体的活性肽及其制备方法和应用 - Google Patents

一种抑制ampa受体的活性肽及其制备方法和应用 Download PDF

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Publication number
CN107428800A
CN107428800A CN201580077695.6A CN201580077695A CN107428800A CN 107428800 A CN107428800 A CN 107428800A CN 201580077695 A CN201580077695 A CN 201580077695A CN 107428800 A CN107428800 A CN 107428800A
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China
Prior art keywords
active peptide
preparation
egar
tetrapeptide
enzymatic hydrolysis
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CN201580077695.6A
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CN107428800B (zh
Inventor
蔡木易
顾怀宇
鲁军
潘兴昌
蔡松
马勇
段松伟
陈亮
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Chengde Xiannongke Biological Technology Co ltd
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Chengde Xiannongke Biological Technology Co ltd
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1021—Tetrapeptides with the first amino acid being acidic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/08—Antiepileptics; Anticonvulsants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22002—Papain (3.4.22.2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

本申请提供了一种抑制AMPA受体的活性肽及其制备方法和应用。所述活性肽的制备方法包括如下步骤：1)将鲑鱼皮浸泡后破碎，加水打浆，调pH值至6.5‑7.5；2)采用中性蛋白酶进行第一酶解；3)采用木瓜蛋白酶进行第二酶解后灭酶；4)将酶解液离心后，对上清液进行膜过滤、浓缩、脱色，制得活性肽。所述活性肽中含有氨基酸序列为Glu‑Gly‑Ala‑Arg的四肽。该四肽的溶解性好，可选择性地抑制神经元由AMPA受体引起的突触传递，具有抗癫痫作用。

Description

PCT国内申请，说明书已公开。

Claims (11)

  1. PCT国内申请，权利要求书已公开。
CN201580077695.6A 2015-07-08 2015-07-08 一种抑制ampa受体的活性肽及其制备方法和应用 Active CN107428800B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/083571 WO2017004811A1 (zh) 2015-07-08 2015-07-08 一种抑制ampa受体的活性肽及其制备方法和应用

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CN107428800A true CN107428800A (zh) 2017-12-01
CN107428800B CN107428800B (zh) 2020-11-27

Family

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Country Status (4)

Country Link
US (1) US10800810B2 (zh)
JP (1) JP6670922B2 (zh)
CN (1) CN107428800B (zh)
WO (1) WO2017004811A1 (zh)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001200000A (ja) * 2000-01-24 2001-07-24 Air Water Inc 海洋生物由来コラーゲンの製造方法
CN1382806A (zh) * 2002-05-15 2002-12-04 中国科学院上海有机化学研究所 深海鱼皮组织工程用胶原蛋白和制备方法
CN1781933A (zh) * 2004-12-01 2006-06-07 中国人民解放军军事医学科学院毒物药物研究所 胸腺素α1活性片段环肽类似物及其聚乙二醇化衍生物
CN101061827A (zh) * 2006-04-30 2007-10-31 中国食品发酵工业研究院 从鱼皮、鱼骨中酶法制取鱼胶原肽的工业生产方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0129260D0 (en) * 2001-12-06 2002-01-23 Eisai London Res Lab Ltd Pharmaceutical compositions and their uses
JP2005053798A (ja) * 2003-08-04 2005-03-03 Nonogawa Shoji Kk フィブロネクチン生成促進剤
JP2005314265A (ja) * 2004-04-28 2005-11-10 Nippon Menaade Keshohin Kk 老化防止剤
JP2007039394A (ja) * 2005-08-04 2007-02-15 Nippon Menaade Keshohin Kk 老化予防改善皮膚外用剤
EP1857463A1 (en) * 2006-05-03 2007-11-21 Ebewe Pharma Ges.m.b.H. Nfg. KG Peptide having neuroprotective effects
JP4437237B2 (ja) * 2007-09-11 2010-03-24 株式会社日本バリアフリー 有用組成物の製造方法
CN101240312A (zh) * 2008-01-21 2008-08-13 南昌大学 一种源于鱼皮的ace抑制肽的制备方法
IE20120542A1 (en) * 2012-12-19 2014-07-02 Provost Fellows Foundation Scholars And Other Members Of Board Of The College Of The Holy And Undivi Novel TRH binding site in human CNS
CN103205480B (zh) * 2013-04-15 2014-10-15 武汉工业学院 一种用鱼皮或鱼骨生产胶原蛋白低聚肽的方法
CN103238870A (zh) * 2013-05-14 2013-08-14 福建农林大学 一种即食鱼皮胶冻食品的制备方法
CN104694615A (zh) * 2015-03-03 2015-06-10 江南大学 一种利用益生菌发酵海洋生物加工下脚料制备降血压肽的方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001200000A (ja) * 2000-01-24 2001-07-24 Air Water Inc 海洋生物由来コラーゲンの製造方法
CN1382806A (zh) * 2002-05-15 2002-12-04 中国科学院上海有机化学研究所 深海鱼皮组织工程用胶原蛋白和制备方法
CN1781933A (zh) * 2004-12-01 2006-06-07 中国人民解放军军事医学科学院毒物药物研究所 胸腺素α1活性片段环肽类似物及其聚乙二醇化衍生物
CN101061827A (zh) * 2006-04-30 2007-10-31 中国食品发酵工业研究院 从鱼皮、鱼骨中酶法制取鱼胶原肽的工业生产方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘文颖 等: "海洋胶原低聚肽的制备及其稳定性研究", 《食品工业》 *

Also Published As

Publication number Publication date
CN107428800B (zh) 2020-11-27
JP2018521650A (ja) 2018-08-09
US10800810B2 (en) 2020-10-13
JP6670922B2 (ja) 2020-03-25
US20180094028A1 (en) 2018-04-05
WO2017004811A1 (zh) 2017-01-12

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