CN107410103B - A method of improving Sepiella maindroni survival rate of seedling - Google Patents
A method of improving Sepiella maindroni survival rate of seedling Download PDFInfo
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- CN107410103B CN107410103B CN201710234205.XA CN201710234205A CN107410103B CN 107410103 B CN107410103 B CN 107410103B CN 201710234205 A CN201710234205 A CN 201710234205A CN 107410103 B CN107410103 B CN 107410103B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to agriculture mariculture technologies, disclose a kind of method for improving Sepiella maindroni survival rate of seedling, comprising the following steps: (1) incubating oosperm；(2) young feeds；(3) seedling separation is cultivated；(4) fresh and alive feedstuff feeding domestication；(5) later stage temperature control is formed.The method of the present invention is directed to the physiological property of Sepiella maindroni, and the domestication of chilled miscellaneous shrimp can be completed in 5 days, and is not in that batch is dead and autotomy phenomenon, survival rate of seedling can be increased to 50% from 20%.The method of the present invention has using easy, economical and practical feature, effectively increases Sepiella maindroni survival rate of seedling.
Description
Technical field
The present invention relates to agriculture mariculture technology more particularly to a kind of sides for improving Sepiella maindroni survival rate of seedling
Method.
Background technique
Sepiella maindroni (Sepiella maindroni de Rochebrune) is commonly called as inkfish, mesh fish, is under the jurisdiction of soft
Body animal door, Cephalopoda, Decapoda, Sepiidae, sepiella maindroni de Rochebrune category.Sepiella maindroni is distributed widely in coastal area of china, Russia sieve
This Yuan Donghai, Japanese Seto Island Sea and the sea areas such as Korea southwest is coastal, be once China, fishing ground, the East Sea traditional " four big marine products " it
One.At the beginning of the last century 80's, since spring flood catches bosom ovum cuttlefish in spawning ground periphery, summer floods catch oviposition cuttlefish in spawning ground
And destroy spawning ground, the winter fishing season fishing hairtail when it is simultaneous catch young cuttlefish and overwintering cuttlefish, cause resource severely damaged, yield is continuous
Decline, the middle and later periods eighties, Along Zhejiang Coast fishing ground Sepiella maindroni yield sharply decline, and can not form fishing season, endangered.
Enhancement releasing is the effective means for increasing the amount of marketable aquatic animals which can be caught to supplement fishery resources, and Sepiella maindroni is fast because growing, the breeding cycle
It is short, it is ideal enhancement species.The beginning of this century, our province department in charge of fisheries start in the Dongji sea of Along Zhejiang Coast
Domain, Taizhou Dachen sea area, Wenzhou Dongtou sea area start to release Sepiella maindroni fertilized eggs and the young, and stock number gradually starts
Restore, annual output is at 3000 tons or so at present.In recent years, with the propulsion and implementation of Zhejiang Fishing Grounds reparation development plan, Zhejiang
Department in charge of fisheries is saved using Sepiella maindroni and Larimichthys crocea as main enhancement releasing kind.Carry out large-scale Mans without
The artificial breeding of Sepia andreana provides Sepiella maindroni seed and the fertilized eggs of high-quality abundance for enhancement releasing, is Zhejiang Fishing Grounds
Repair the guarantee that development plan is smoothly implemented.Currently, having carried out Sepiella maindroni in the Fujian in China and Zhejiang area
Artificial breeding and cultivation, but the survival rate in Sepiella maindroni seedling in cultivating process is lower always, only 20% or so, reason
That Sepiella maindroni seed is advisable with living bait of ingesting, domestication chilled bait of ingesting is more difficult, sufficient living bait it is difficult to ensure that,
Sepiella maindroni has autotomy characteristic simultaneously, and Gu Yumiaochangjia will appear large quantities of death.
So completing the conversion from living bait to chilled bait as early as possible in Sepiella maindroni seedling raising process, avoid certainly
Residual, later period cultivation stages control water temperature, and establishing a set of practicable Sepiella maindroni offspring seed cultivation method is that raising is graceful
The key point and problem to be solved by this invention of family name's sepiella maindroni de Rochebrune survival rate of seedling.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides a kind of sides for improving Sepiella maindroni survival rate of seedling
Method.The method of the present invention is directed to the physiological property of Sepiella maindroni, the domestication of chilled miscellaneous shrimp can be completed in 5 days, and will not go out
Now batch is dead and autotomy phenomenon, survival rate of seedling can be increased to 50% from 20%.The method of the present invention has using easy, economy
Practical feature effectively increases Sepiella maindroni survival rate of seedling.
The specific technical proposal of the invention is: a kind of method for improving Sepiella maindroni survival rate of seedling, including it is following
Step:
(1) incubating oosperm: choosing that ovum grain is full, Sepiella maindroni fertilized eggs of the pitch-black light of color, is put into
Hatched in hatchery, breeding in culture pond is transferred to after hatching；
(2) young feeds: 2 age in days of the young starts to feed artemia nauplii, persistently feeds 20-25 days；The young 15-25 days
Age starts to feed Copepods living body；20 age in days of the young starts oppossum shrimp living body；
(3) seedling separation is cultivated: after 30 age in days of the young and the young is averaged trunk when 1cm, the lesser young is transferred to new
It is cultivated in culture pond, using the breeding method of the culture 25-30 age in days young, so that the young ratio of original Chi Zhong little is less than 5%；Together
When, the young in former pond is subjected to a point pond and is cultivated；
(4) fresh and alive feedstuff feeding domestication: after dividing pond to cultivate, chilled miscellaneous shrimp is fed before and after changing water every morning, is tamed
Oppossum shrimp living body is fed after 1.5-2.5h；It feeds chilled miscellaneous shrimp every night, tames 25-35min, persistently tame 4-6 days；Wait feed
Chilled miscellaneous shrimp when thering is 78-82% to be ingested by the young, stop daytime feeding oppossum shrimp living body, reduce oppossum shrimp living body at night and feed, wait throw
When the chilled miscellaneous shrimp fed has 88-92% to be ingested by the young, consider to stop feeding oppossum shrimp living body, all feeds chilled miscellaneous shrimp raising；
(5) later stage temperature control is formed: before young weight reaches 48-52g, forming water temperature control at 23 ± 2 DEG C；In children
When body weight reaches 48-52g or more, water temperature is formed no more than 22-23 DEG C.
The present invention is according to traditional Sepiella maindroni offspring seed cultivation method and combines Sepiella maindroni seed physiology raw
State characteristic uses: one, collecting high quality fertilized egg of scophthatmus and concentrate hatching, every pond breeding time is no more than 5 days；Two, extend artemia without section
The young and oppossum shrimp Feeding time；Three, it is divided into seedlings cultivation in time by the long size of trunk；Four, fresh and alive feed is tamed in due course；Five, the later period forms
The methods of stage control water temperature improves Sepiella maindroni weed survival rate to complete Sepiella maindroni seed rearing.
Preferably, step (1) method particularly includes:
Choose that 60kg ovum grain is full, Sepiella maindroni fertilized eggs of the pitch-black light of color, with the formaldehyde dipping of 100ppm
15min removes the microorganism on fertilized eggs surface；Then fertilized eggs are averagely fitted into large bamboo or wicker basket and are suspended in hatchery, each square-bottomed bamboo basket
Basket places 3kg fertilized eggs；Wherein, 15 m of water body of hatchery3, Spawning water is sand leaching water, salinity 23~24, using aerated flow
Water is hatched, and early period day, streamflow was 200%, and later period day streamflow is 300%, 23 ± 2 DEG C of water temperature, does 20 min of dew from water daily,
Every 6 days of the mid-early stage of hatching by fertilized eggs together with the formaldehyde dipping 15min of large bamboo or wicker basket 100ppm；Start to occur after 22-23 days
Rupture of membranes, the Newly hatchled for starting small lot are not used in breeding, when the Newly hatchled quantity in spawning pond reaches 5000 ind, and it is big
When most fertilized eggs have entered the hatching rupture of membranes stage, start to start breeding with the etting interception discharge outlet of 20 mesh, while using upper
Accelerate rupture of membranes speed in noon and each dry 20 min of dew fertilized eggs 1 time of afternoon；Artemia nauplii is fed, feeding volume is 0.1 ind
/ mL, when young density reaches 3000-4000 ind/m in hatchery3When, fertilized eggs are moved on into breeding in culture pond, stop the supple of gas or steam 3-
After 5min when in the upper floatation surface of squid larva major part or water in suspension, inflation will be opened after the suction of bottom of pond portion dirt with dirt sucking device
The micro- inflation of valve, while 50% is drained, new water is added and enters the larval culture stage.The breeding time is no more than 5 days.
Preferably, step (2) method particularly includes:
The young is cultivated under 23 ± 2 DEG C of water temperature conditions, inflation, salinity 23~24, using miniflow water and the training for changing water combination
The mode of supporting, day streamflow are 20%, day quantity of exchanged water 50%, daily soil pick-up 1 time；2 ages in days start to feed artemia nauplii, feeding volume
For 0.1 ind/mL, persistently feed 20-25 days；15-25 age in days starts to feed Copepods living body, feeding volume be 0.05 ind/
mL；20 ages in days start to feed oppossum shrimp living body；Wherein, miscellaneous shrimp therein is screened out with the mesh screen of 4 mesh before feeding oppossum shrimp living body, simultaneously
With sand filtration seawater flushing it is clean after feed, feeding volume be 3-5 ind/L, guarantee water body in have a certain amount of oppossum shrimp, until complete
Chilled miscellaneous shrimp domestication.
Preferably, step (3) method particularly includes: it is cultivated under 23 ± 2 DEG C of water temperature conditions, up to 30 ages in days and culture pond
In the young be averaged trunk when the 1cm, individual lesser in culture pond is pulled out with water ladle band water, moves into new culture pond and trains
It supports, using the breeding method of the culture 25-30 age in days young, so that the individual ratio of original Chi Zhong little is less than 5%；Meanwhile it will be in former pond
The young carry out a point pond and cultivate, density domination is in 1500-2000 ind/m3, using miniflow water and the culture side for changing water combination
Formula, day streamflow are 30%, day quantity of exchanged water 70%, daily soil pick-up 1 time.
Preferably, step (4) method particularly includes: after dividing pond to cultivate, start domestication and feed chilled miscellaneous shrimp, daily early
On change before oppossum shrimp living body before and after water is fed and open domestication, feeding volume increases day by day, and domestication every morning, 2h time is thrown after domestication
Oppossum shrimp living body is fed, feeding volume is 3-5 ind/L；Using miniflow water and the training method for changing water combination, day streamflow is 30%, day
Quantity of exchanged water 70%, daily soil pick-up 1 time；Meanwhile the fluorescent lamp of one 100w is set in a corner of nursery pond, light bulb is apart from water
Face 40cm, darkness back light photoinduction 10min, under the synchoropaedia to light in nursery pond, feed chilled miscellaneous shrimp, feeding volume by
Day increases；Wherein, daily light induction cluster tames 30min, with siphonage by the residual bait under light in bottom of pond after domestication
It is sucked out, persistently tames 5 days altogether；Chilled miscellaneous shrimp wait feed has 80% when being ingested, and daytime, stopping fed oppossum shrimp living body, subtracted at night
Oppossum shrimp living body is fed less, and the chilled miscellaneous shrimp wait feed has 90% when being ingested, and considers to stop feeding oppossum shrimp living body, all using throwing
Feed chilled miscellaneous shrimp raising.
In the methods of the invention, the physiological property for making full use of the Sepiella maindroni young, makes full use of the time, white
It, at night it is tamed respectively, shorten domestication the time.According to the method described above, taming and dociling for chilled miscellaneous shrimp can be completed in 5 days
Change, and is not in that batch is dead and autotomy phenomenon, survival rate of seedling can be improved to 50%.
Preferably, the main species of the chilled miscellaneous shrimp are red huge legendary turtle shrimp and Palaemon gravieri in step (4), -18 DEG C add
Water stored frozen；The long shrimp for being less than 1cm of body in the miscellaneous shrimp of screening before feeding.
The Sepiella maindroni seedling domestication miscellaneous shrimp of ice shrimp used is the miscellaneous shrimp captured in the island Xi Shan sea area, and main species are
Red huge legendary turtle shrimp and Palaemon gravieri have in liberal supply, cheap, the fresh advantage of quality.
Preferably, miscellaneous shrimp chilled to part is by pre- place before feeding when domestication feeds chilled miscellaneous shrimp in step (4)
Reason: compound concentration is the chitosan suspension of 4-8wt%, 1-10 in mass ratio after grinding from the internal organ of fresh taking-up in shrimp living:
100 are added in chitosan suspension and stir evenly spare；Chilled miscellaneous shrimp is subjected to centrifugal dehydration treatment；Then by chitosan
Suspension is applied to chilled miscellaneous shrimp surface and forms a tunic, most afterwards after being dried can with without pretreated chilled miscellaneous
It is fed after shrimp doping.
People's experience and long-term research according to the present invention, squid larva is to living body grazing rate height and to chilled miscellaneous shrimp
The low reason of (dead volume) grazing rate has two aspects: one, living body is suspended in water body, and can be movable, and dead volume puts into water
In after can settle, and will not activity, squid larva is stronger to the observation of levitated object and mobiles, therefore ingests
Rate is high；Two, the freshness of living body is more preferable, and squid larva has a preference for fresh material of ingesting.According to above-mentioned discovery, the present inventor is to part
Chilled miscellaneous shrimp first passes through dehydration in advance, so that its density reduces, while cooperating the aeration of bottom of pond upward direction, after putting into water
Will not rapid subsidence, increase the residence time in water, be easy to be found by squid larva.On the other hand, after dewatering chilled
Miscellaneous shrimp smears the fresh internal organ of work shrimp for playing attractant effect, and the fresh internal organ of shrimp living are complexed with chitosan.Chitosan
Water solubility it is poor, in water in cotton-shaped, and good to the complexing of the fresh internal organ of shrimp living.The chitosan suspension of high concentration forms a film
Property it is good, be easy to form a film after being applied to chilled miscellaneous shrimp surface, and put into water after drying and can play barrier to a certain extent
Effect, slows down the infiltration of water, further delays the sinking speed of bait.And the fresh internal organ of the shrimp that lives are extracted from new fresh and alive shrimp, newly
Freshness is high, strong to the attractant of the young.In addition, being first dehydrated in chilled miscellaneous shrimp, then smeared, then in drying, although comparing
First smearing property dehydration again will grow, but can be avoided film layer centrifugation caused by centrifugal dehydration after first smearing and fall off.
And centrifugal dehydration again after chilled miscellaneous shrimp surface filming, internal moisture are difficult to effectively be detached from due to the barrier of film layer.
Preferably, during feeding chilled miscellaneous shrimp in step (4), by pretreated chilled miscellaneous shrimp doping day by day
It reduces.After squid larva gradually forms the habit for dead volume bait of ingesting, gradually decrease by pretreated chilled miscellaneous shrimp, with for
The unpretreated chilled miscellaneous shrimp that feeds for a long time in later period is prepared.
Preferably, step (5) method particularly includes: before young weight reaches 48-52g, water temperature control is formed in 24-25
℃；When young weight reaches 48-52g or more, forms water temperature and be no more than 22-23 DEG C, by the way of big aerated flow Aquaponic, day
Streamflow is 150%, and the feeding volume of daily soil pick-up 1 time, chilled miscellaneous shrimp is determined according to residual bait amount, not occur residual bait and autotomy
Subject to.
It is compared with the prior art, the beneficial effects of the invention are as follows the present invention according to traditional Sepiella maindroni seed rearing
Method simultaneously combines Sepiella maindroni seed physio-ecology characterristics, uses: one, collecting high quality fertilized egg of scophthatmus and concentrate hatching, every pond
The breeding time is no more than 5 days；Two, extend artemia nauplii and oppossum shrimp Feeding time；Three, it is divided into seedlings training in time by the long size of trunk
It educates；Four, fresh and alive feed is tamed in due course；Five, the methods of later period forming stage control water temperature, to complete the training of Sepiella maindroni seed
It educates, improves Sepiella maindroni weed survival rate.
Specific embodiment
The present invention will be further described with reference to the examples below.
Embodiment 1
Test site is dodged in Zhejiang Prov. Inst. of Marine Products west and carries out following tests in October, 2015 in June, 2016,
In, Copepods living body is that voluntarily Tu Chi is cultivated at Zhejiang Prov. Inst. of Marine Products test site；Oppossum shrimp living body is Zhejiang Province's ocean water
Produce research institute test site voluntarily uses light to capture after Tu Chi is cultivated.Chilled miscellaneous shrimp is purchased from the trawler in the island Xi Shan sea area, mainly
Type is red huge legendary turtle shrimp and Palaemon gravieri, is frozen in the freezer that test site is dodged in west, it is desirable that -18 DEG C plus water stored frozen.
A method of improving Sepiella maindroni survival rate of seedling, comprising the following steps:
(1) incubating oosperm: choosing that 60kg ovum grain is full, Sepiella maindroni fertilized eggs of pitch-black light of color, use
The formaldehyde dipping 15min of 100ppm removes the microorganism on fertilized eggs surface；Then fertilized eggs are averagely fitted into large bamboo or wicker basket and are suspended on
In hatchery, each large bamboo or wicker basket places 3kg fertilized eggs；Wherein, 15 m of water body of hatchery3, Spawning water is sand leaching water, salinity 23
~24, using inflation Lotic hatching, early period, day streamflow was 200%, and later period day streamflow is 300%, 23 ± 1 DEG C of water temperature, daily
From water 20 min of dry dew, every 6 days of the mid-early stage of hatching by fertilized eggs together with the formaldehyde dipping 15min of large bamboo or wicker basket 100ppm；22-
Start rupture of membranes occur after 23 days, the Newly hatchled for starting small lot is not used in breeding, when the Newly hatchled quantity in spawning pond reaches
To 5000 ind, and when most of fertilized eggs have entered the hatching rupture of membranes stage, start to be started with the etting interception discharge outlet of 20 mesh
Breeding, while using the morning and each dry 20 min of dew fertilized eggs 1 time of afternoon, quickening rupture of membranes speed；Artemia nauplii is fed,
Feeding volume is 0.1 ind/mL, when young density reaches 3000-4000 ind/m in hatchery3When, fertilized eggs are moved on into training
Breeding in pond is educated, it is with dirt sucking device that bottom of pond portion is dirty after the 4min that stops the supple of gas or steam when in the upper floatation surface of squid larva major part or water in suspension
Object opens the micro- inflation of charging valve after being sucked out, while draining 50%, and new water is added and enters the larval culture stage；The breeding time does not surpass
Spend 5 days.
(2) young feeds: the young is cultivated under 23 ± 1 DEG C of water temperature conditions, micro- inflation, salinity 23~24, using miniflow water
With the training method for changing water combination, day streamflow is 20%, day quantity of exchanged water 50%, daily soil pick-up 1 time；2 ages in days start to feed artemia
Nauplius, feeding volume are 0.1 ind/mL, are persistently fed 20-25 days；15-25 age in days starts to feed Copepods living body, feeds
Amount is 0.05 ind/mL；20 ages in days start to feed oppossum shrimp living body；Wherein, feeding will wherein with the mesh screen of 4 mesh before oppossum shrimp living body
Miscellaneous shrimp screen out, while with sand filtration seawater flushing it is clean after feed, feeding volume be 4 ind/L, guarantee water body in have it is a certain amount of
Oppossum shrimp, until completing chilled miscellaneous shrimp domestication.
(3) seedling separation is cultivated: it is cultivated under 23 ± 1 DEG C of water temperature conditions, it is long up to the trunk that is averaged of the young in 30 ages in days and culture pond
When up to 1cm, individual lesser in culture pond is pulled out with water ladle band water, moves into new culture pond and cultivates, using culture 25-30
The breeding method of the age in days young, so that the individual ratio of original Chi Zhong little is less than 5%；Meanwhile the young in former pond is subjected to a point Chi Pei
It supports, density domination is in 1500-2000 ind/m3, using miniflow water and the training method for changing water combination, day streamflow is 30%,
Day quantity of exchanged water 70%, daily soil pick-up 1 time.
(4) fresh and alive feedstuff feeding domestication: after dividing pond to cultivate, start domestication and feed chilled miscellaneous shrimp, before every morning changes water
Oppossum shrimp living body opens domestication afterwards before feeding, and feeding volume increases day by day, and every morning tames time 2h, and it is living that oppossum shrimp is fed after domestication
Body, feeding volume are 4 ind/L；Using miniflow water and change the training method of water combination, day streamflow is 30%, day quantity of exchanged water 70%,
Daily soil pick-up 1 time；Meanwhile the fluorescent lamp of one 100w is set in a corner of nursery pond, light bulb is apart from water surface 40cm, darkness
Back light photoinduction 10min, under the synchoropaedia to light in nursery pond, feed chilled miscellaneous shrimp, feeding volume increases day by day；Its
In, daily light induction cluster tames 30min, and the residual bait under light in bottom of pond is sucked out with siphonage after domestication, holds altogether
Continuous domestication 5 days；Chilled miscellaneous shrimp wait feed has 80% when being ingested, and stops daytime feeding oppossum shrimp living body, reduces feed oppossum shrimp at night
Living body, the chilled miscellaneous shrimp wait feed have 90% when being ingested, and consider to stop feeding oppossum shrimp living body, all using feeding chilled miscellaneous shrimp
Raising.
Wherein, the main species of the chilled miscellaneous shrimp are red huge legendary turtle shrimp and Palaemon gravieri, -18 DEG C plus water stored frozen；It throws
The long shrimp for being less than 1cm of body in the miscellaneous shrimp of screening before feeding.And miscellaneous shrimp chilled to part is by pretreatment before feeding: compound concentration
For the chitosan suspension of 6wt%, chitosan will be added to from 5:100 in mass ratio after the internal organ grinding of fresh taking-up in shrimp living
In suspension and stir evenly spare；Chilled miscellaneous shrimp is subjected to centrifugal dehydration treatment；Then chitosan suspension is applied to ice
Fresh miscellaneous shrimp surface forms a tunic, most afterwards after being dried can with without being fed after pretreated chilled miscellaneous shrimp doping.
And during feeding chilled miscellaneous shrimp, reduced day by day by the doping of pretreated chilled miscellaneous shrimp.
(5) later stage temperature control is formed: when young weight reaches 50g or more, being formed water temperature no more than 22-23 DEG C, is adopted
With the mode of big aerated flow Aquaponic, day streamflow is 150%, and daily soil pick-up 1 time, the feeding volume of chilled miscellaneous shrimp is according to residual bait amount
It determines, is subject to and does not occur residual bait and autotomy.
Embodiment 2
The present embodiment difference from example 1 is that: for accelerate Sepiella maindroni larval growth speed, early period
The temperature that (young weight reaches before 50g) cultivates can be properly increased to 24-25 DEG C.
Sepiella maindroni seed breeding is carried out by means of the present invention, and the domestication of chilled miscellaneous shrimp can be completed in 5 days,
50% can be increased to from existing 20% by not occurring batch death and autotomy phenomenon, survival rate of seedling.Mans needleless crow is cultivated altogether
Thief releases parent 100,000, produces fertilized eggs 50,000,000.This method, which has, uses simplicity, economical and practical, effectively increases graceful
Family name's sepiella maindroni de Rochebrune survival rate of seedling.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present invention
Method therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not intended to limit the invention in any way, it is all according to the present invention
Technical spirit any simple modification, change and equivalent transformation to the above embodiments, still fall within the technology of the present invention side
The protection scope of case.
Claims (8)
1. a kind of method for improving Sepiella maindroni survival rate of seedling, it is characterised in that the following steps are included:
(1) incubating oosperm: choosing that ovum grain is full, Sepiella maindroni fertilized eggs of the pitch-black light of color, is put into hatching
Hatched in pond, breeding in culture pond is transferred to after hatching；
(2) young feeds: the young is cultivated under 23 ± 2 DEG C of water temperature conditions, inflation, and salinity 23~24 ‰ using miniflow water and are changed
The training method that water combines, day streamflow are 20%, day quantity of exchanged water 50%, daily soil pick-up 1 time；2 ages in days start to feed artemia without section
The young, feeding volume are 0.1 ind/mL, are persistently fed 20-25 days；15-25 age in days starts to feed Copepods living body, and feeding volume is
0.05 ind /mL；20 ages in days start to feed oppossum shrimp living body；Wherein, feeding will be therein miscellaneous with the mesh screen of 4 mesh before oppossum shrimp living body
Shrimp screens out, at the same with sand filtration seawater flushing it is clean after feed, feeding volume be 3-5 ind/L, guarantee water body in have a certain amount of chaff
Shrimp living body, until completing chilled miscellaneous shrimp domestication；
(3) seedling separation is cultivated: after 30 age in days of the young and the young is averaged trunk when 1cm, and the lesser young is transferred to new cultivation
It is cultivated in pond, using the breeding method of the culture 25-30 age in days young, so that the young ratio of original Chi Zhong little is less than 5%；Meanwhile it will
The young in former pond carries out a point pond and cultivates；
(4) fresh and alive feedstuff feeding domestication: after dividing pond to cultivate, chilled miscellaneous shrimp is fed before and after changing water every morning, tames 1.5-
Oppossum shrimp living body is fed after 2.5h；It feeds chilled miscellaneous shrimp every night, tames 25-35min, persistently tame 4-6 days；Ice to be fed
When fresh miscellaneous shrimp has 78-82% to be ingested by the young, stops daytime feeding oppossum shrimp living body, reduce oppossum shrimp living body at night and feed, it is to be fed
When chilled miscellaneous shrimp has 88-92% to be ingested by the young, stopping feeds oppossum shrimp living body, all feeds chilled miscellaneous shrimp raising；
(5) later stage temperature control is formed: before young weight reaches 50g, forming water temperature control at 23 ± 2 DEG C；In young weight
When up to 50g or more, water temperature is formed no more than 23 DEG C.
2. a kind of method for improving Sepiella maindroni survival rate of seedling as described in claim 1, which is characterized in that step
(1) method particularly includes:
Choose that 60kg ovum grain is full, Sepiella maindroni fertilized eggs of the pitch-black light of color, with the formaldehyde dipping of 100ppm
15min removes the microorganism on fertilized eggs surface；Then fertilized eggs are averagely fitted into large bamboo or wicker basket and are suspended in hatchery, each square-bottomed bamboo basket
Basket places 3kg fertilized eggs；Wherein, 15 m of water body of hatchery3, Spawning water is sand leaching water, salinity 23~24 ‰, using inflation
Lotic hatching, early period day, streamflow was 200%, and later period day streamflow is 300%, 23 ± 2 DEG C of water temperature, did dew 20 from water daily
Min, every 6 days of the mid-early stage of hatching by fertilized eggs together with the formaldehyde dipping 15min of large bamboo or wicker basket 100ppm；Start after 22-23 days
There is rupture of membranes, the Newly hatchled for starting small lot is not used in breeding, when the Newly hatchled quantity in spawning pond reaches 5000 ind,
And most of fertilized eggs start to start breeding with the ettings of 20 mesh interception discharge outlet, adopt simultaneously when having entered the hatching rupture of membranes stage
Each dry 20 min of dew fertilized eggs 1 time with the morning and afternoon, accelerate rupture of membranes speed；Feed artemia nauplii, feeding volume 0.1
Ind/mL, when young density reaches 3000-4000 ind/m in hatchery3When, fertilized eggs are moved on into breeding in culture pond, are stopped
After gas 3-5min when in the upper floatation surface of squid larva major part or water in suspension, it will be opened after the suction of bottom of pond portion dirt with dirt sucking device
The micro- inflation of charging valve, while 50% is drained, new water is added and enters the larval culture stage；The breeding time is no more than 5 days.
3. a kind of method for improving Sepiella maindroni survival rate of seedling as described in claim 1, which is characterized in that step
(3) method particularly includes: it is cultivated under 23 ± 2 DEG C of water temperature conditions, is averaged trunk up to 1cm up to the young in 30 ages in days and culture pond
When, individual lesser in culture pond is pulled out with water ladle band water, moves into new culture pond and cultivates, using culture 25-30 age in days
The breeding method of the young, so that the individual ratio of original Chi Zhong little is less than 5%；Meanwhile the young in former pond is subjected to a point pond and is cultivated,
Density domination is in 1500-2000 ind/m3, using miniflow water and the training method for changing water combination, day streamflow is 30%, and day changes
Water 70%, daily soil pick-up 1 time.
4. a kind of method for improving Sepiella maindroni survival rate of seedling as described in claim 1, which is characterized in that step
(4) method particularly includes:
After dividing pond culture, starts domestication and feed chilled miscellaneous shrimp, oppossum shrimp living body is tamed before feeding before and after changing water every morning, is fed
Amount increases day by day, and every morning tames time 2h, and oppossum shrimp living body is fed after domestication, and feeding volume is 3-5 ind/L；Using
Miniflow water and the training method for changing water combination, day streamflow are 30%, day quantity of exchanged water 70%, daily soil pick-up 1 time；Meanwhile in nursery
The fluorescent lamp of a 100w is arranged in one corner in pond, and light bulb is apart from water surface 40cm, darkness back light photoinduction 10min, to nursery
Under synchoropaedia to light in pond, chilled miscellaneous shrimp is fed, feeding volume increases day by day；Wherein, daily light induction cluster domestication
Residual bait under light in bottom of pond is sucked out after domestication with siphonage, is persistently tamed 5 days altogether by 30min；To be fed is chilled miscellaneous
Shrimp has 80% when being ingested, and stops daytime feeding oppossum shrimp living body, reduces feed oppossum shrimp living body at night, chilled miscellaneous shrimp to be fed has
90% when being ingested, and stopping feeds oppossum shrimp living body, all using feeding chilled miscellaneous shrimp raising.
5. a kind of method for improving Sepiella maindroni survival rate of seedling as claimed in claim 4, which is characterized in that step
(4) in, the main species of the chilled miscellaneous shrimp are red huge legendary turtle shrimp and Palaemon gravieri, -18 DEG C plus water stored frozen；Feed preceding screening
The long shrimp for being less than 1cm of body in miscellaneous shrimp.
6. a kind of method of raising Sepiella maindroni survival rate of seedling as described in claim 1 or 4 or 5, which is characterized in that
When domestication feeds chilled miscellaneous shrimp in step (4), miscellaneous shrimp chilled to part is by pretreatment before feeding: compound concentration 4-
The chitosan suspension of 8wt% will be added to chitosan from 1-10:100 in mass ratio after the internal organ grinding of fresh taking-up in shrimp living
In suspension and stir evenly spare；Chilled miscellaneous shrimp is subjected to centrifugal dehydration treatment；Then chitosan suspension is applied to ice
Fresh miscellaneous shrimp surface forms a tunic, most afterwards after being dried can with without being fed after pretreated chilled miscellaneous shrimp doping.
7. a kind of method for improving Sepiella maindroni survival rate of seedling as claimed in claim 6, which is characterized in that in step
(4) it during feeding chilled miscellaneous shrimp in, is reduced day by day by the doping of pretreated chilled miscellaneous shrimp.
8. a kind of method for improving Sepiella maindroni survival rate of seedling as described in claim 1, which is characterized in that step
(5) method particularly includes:
Before young weight reaches 50g, water temperature control is formed at 24-25 DEG C；When young weight reaches 50g or more, water temperature is formed not
More than 23 DEG C, by the way of big aerated flow Aquaponic, day streamflow is 150%, daily soil pick-up 1 time, the feeding volume of chilled miscellaneous shrimp
It is determined according to residual bait amount, is subject to and does not occur residual bait and autotomy.
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