CN107409921A - A kind of method of yellow pear tree cuttage and seedling culture - Google Patents

A kind of method of yellow pear tree cuttage and seedling culture Download PDF

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Publication number
CN107409921A
CN107409921A CN201710748132.6A CN201710748132A CN107409921A CN 107409921 A CN107409921 A CN 107409921A CN 201710748132 A CN201710748132 A CN 201710748132A CN 107409921 A CN107409921 A CN 107409921A
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cuttage
parts
branch
pear tree
culture
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汪善洋
殷辉
高正辉
黄学红
王朝向
王金泉
张卫峰
薛炳杰
范孝勤
张焱
许松
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Funan County Agriculture Fruit Co Ltd
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Funan County Agriculture Fruit Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40—Liliopsida [monocotyledons]
    • A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
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Abstract

The invention discloses a kind of method of yellow pear tree cuttage and seedling culture, it is characterised in that including following aspect：（1）Branch is selected, 56 years yellow pear tree branches is selected, the 2cm of branch diameter 1, carries out intercepting process；（2）Pretreatment, cuttage branch lower end is wrapped up using the gauze of dipping bactericidal liquid, and carry out ionization process；（3）Dipping is taken root, and cuttage branch is immersed respectively and promotees root-growing agent A and promotees to carry out impregnation in root-growing agent B；（4）Hot-house culture, cuttage branch is inserted in nutrient solution test tube, adjust warm indoor temperature, red blue light shines and magnetizing parameters carry out hot-house culture；（5）Greenhouse is transplanted, and cuttage seedling replanting is carried out to greenhouse plantation culture into greenhouse soil.The yellow pear tree that the inventive method is cultivated, compared with control group, its rooting rate improves 15.10%, and its survival rate improves 18.12%, and the 2nd year single yield is more than 9.53kg, and sugar content improves 3.90%.

Description

A kind of method of yellow pear tree cuttage and seedling culture
Technical field
The invention belongs to planting fruit trees technical field, and in particular to a kind of method of yellow pear tree cuttage and seedling culture.
Background technology
Yellow pears are the famous pears quality in China, its have thin skin core it is small, without lithocyte, pulp is fine and smooth, pure white fragrant and sweet spy Point, wherein containing compositions such as abundant protein, fat, minerals and vitamins, have reduce blood pressure, the work(of replenishing the vital essence and removing heat Effect, and there is curative effect to illnesss such as hypertension, heart disease, hepatitis, hepatic sclerosis.Yellow pear Cold region apple type, it, which has, adapts to Property is strong, can be planted in the various soil such as yellow sand, yellow clay, black earth, salt-soda soil, hillside, hills, play sand prevention and consolidate Soil, greening environment, devastated land resource is made full use of, and can also be that planting household brings considerable business efficiency；Yellow pears plantation Enter within the 3rd year high yield period afterwards, enter the best fruiting period within 4 years.And tradition uses seed seedling-raising, grafting and skewer to the implantation methods of yellow pears Insert method, wherein the growth of yellow pears seed seedling-raising is slow, crop cycle is grown, and orchard worker's economic benefit can be caused to decline, and germination Rate is low, cultivates, maintenance cost is high after transplanting；And yellow pears cottage method, need in advance to carry out stock transplanting plantation, add plantation Cost, and yellow pears grafting is had a great influence by factors such as moisture, stock variety, temperature, can cause survival rate, healing life after grafting It is long slow, and Loss occurs in yellow pears inhereditary feature after grafting, and taste quality declines；And cottage method its given birth to Root is lateral root, and root growth, absorbability are relatively weak, and survival rate, the Resistant of yellow pear tree can be caused by directly carrying out transplanting Evil ability, the speed of growth and fruit-growing time length.
The content of the invention
The present invention is directed to the problem of existing：Seed seedling-raising method, wherein the growth of yellow pears seed seedling-raising is slow, crop cycle is grown, Orchard worker's economic benefit can be caused to decline, and percentage of seedgermination is low, cultivates, maintenance cost is high after transplanting；Yellow pears engrafting method, need Transplanting plantation is carried out to stock in advance, adds planting cost, and yellow pears grafting is by factors such as moisture, stock variety, temperature Have a great influence, survival rate after grafting, healing can be caused slow-growing, and yellow pears inhereditary feature occurs that loss is existing after grafting As taste quality declines；And cottage method its to take root be lateral root, root growth, absorbability are relatively weak, directly Survival rate, diseases and insect pests resistance, the speed of growth and the fruit-growing time length of yellow pear tree can be caused by carrying out transplanting.
The present invention is achieved by the following technical solutions：
A kind of method of yellow pear tree cuttage and seedling culture, comprises the following steps：
（1）Select branch：The age of tree is selected in 5-6 Huang pear tree branches, disease and insect resistance and environment adaptation ripe with physiological growth Ability is strong, it is possible to increase cuttage branch rooting rate and survival rate, a diameter of 1-2cm of branch, and intercepting process is carried out to branch, Obtain cuttage branch；
（2）Pretreatment：Cuttage branch bottom is wrapped up after cotton gauze is impregnated in bactericidal liquid, reduces microorganism to cuttage portion corruption Effect, and moisture is kept, and 5-6h is placed in 20-24 DEG C of isoperibol, it is subsequently placed in on-load voltage 36-40V, electric current Ionization process 20-30min in 0.2-0.4A ionization chambers, to cuttage bar cell-stimulating activity, improve growth rate；
（3）Dipping is taken root：Cuttage branch after pretreatment is submerged initially in growth accelerator A, activates cuttage cytoactive, is promoted Branch cell division, 1-2h is soaked under 30-34 DEG C of bath temperature, then soak 3-4h in growth accelerator B, cuttage can be promoted Bar roots and nutrient absorption, and keeps above process chamber exhaust air rate to impregnate cuttage branch in 8-10L/min；
（4）Hot-house culture：It will impregnate in teat glass of the cuttage branch insertion equipped with nutrient solution, be placed on bed of nursing young plants in hothouses and carry out Culture, adjustment greenhouse temperature to 27-32 DEG C of holding 2-3 days, humidity 65%-70%, then temperature is adjusted to 22-25 DEG C carry out temperature Room is cultivated, the red blue light illumination 3-4h/d of 410-440lux, and magnetizes 20-23min per 4-5h using 0.3-0.5MHz magnetizers, is obtained Cuttage seeding；The abundant nutrition composition and active ingredient contained in nutrient solution, it is possible to provide nutrition supply, and improve the speed of growth and contracting It is short to bear fruit the cycle, and magnetize training method, source electrode hormone in plant generation can be stimulated, improves shoot maturation character, shortens plant Fruit-growing time, and improve yield；
（5）Transplant in greenhouse：Cuttage transplantation of seedlings through hot-house culture to greenhouse is planted in soil, planting depth 5-6cm, used Growth accelerator B is colonized, and is poured 1 time using nutrient solution per 5-6 days, and every usage amount is 40-60ml；Cuttage branch root can be improved Portion's maturity, environment-adapting ability and diseases and insect pests resistance, so as to improve transplanting plantation survival rate.
Step（1）Described intercepting process, its intercepted length are 10-14cm, and branch upper end section keeps concordant, and retains 3-4 piece spires, 30 ° of inclinations angle are cut into bottom, and draw 3-4 knives in notch flank vertical direction.
Step（2）Described bactericidal liquid, it is 800 times of dilute solutions of AVM.
Step（2）Described growth accelerator A, wherein CPPU:Zeatin quality proportioning is 2-3:1-2, its mass concentration are 0.3%-0.4%；Described growth accelerator B, wherein heteroauxin：Naa：Gibberellin quality proportioning is 2-3:2:1, its quality Concentration is 0.6%-0.8%.
Step（4）Described nutrient solution, its compound method are：
Zymotic fluid 50-54 parts, plant extraction liquid 21-25 parts, growth accelerator B 5-6 parts are weighed according to quality, is uniformly made after mixing Obtain nutrient solution.
Described zymotic fluid, its compound method are：
According to quality weighing water intaking 100-120 parts, peanut powder 12-15 parts, soybean juice 10-14 parts, pumpkin powder 7-9 parts, purple bamboo leaf powder 4-5 parts, bitter sweet oak powder 3-4 parts, hazelnut powder 1-2 parts, water quality 2%-3% mix bacterium agent is inoculated with after being stirred, is placed in 25-29 DEG C 36-40h is cultivated in fermentation tank, zymotic fluid is made after 120 mesh filter screen filtrations.
Described plant extraction liquid, its compound method are：
Ginkgo leaf 17-23 parts, grape juice 8-12 parts, corn juice 7-9 parts, common crapemyrtle leaf 5-8 parts, Chinese pine leaf 3-5 are weighed according to quality Part, first ginkgo leaf, common crapemyrtle leaf and the efflorescence of Chinese pine leaf are handled, then 3-4 times of gross mass is added after being mixed with grape juice and corn juice Water, boiling 1-2h at a temperature of being placed in 82-87 DEG C, after centrifugal filtration be made plant extraction liquid.
The present invention has advantages below compared with prior art：Cuttage branch selects, and selects 5-6 plant as cuttage branch Elite stand, its physiological growth are ripe, disease and insect resistance and strong environmental adaptability, it is possible to increase cuttage branch rooting rate and survive Rate, branch diameter will be chosen and be limited to 1-2cm, shoot growth fertility is strong, and branch rooting rate is fast, survival rate is high；And 30 ° Angular cutouts and lateral plate designated port, can increase branch lower end and soak, nutrient solution contact area are big, promote cuttage branch take root and Nutritional ingredient absorbs.Preprocess method, branch bottom is wrapped up using the cotton gauze of dipping bactericidal liquid, incision is carried out at sterilization Reason, prevent germ contamination from reducing rooting rate, and be continuously cuttage branch and moisture supply is provided, avoid the excessive plant of dehydration from losing Activity；And ionization method is used, active cell active function can be played to cuttage bar, improves its germinating growth speed, and can Yellow pears are stimulated to produce resistance character.Rooting method is impregnated, cuttage branch is respectively placed in growth accelerator A and B and soaked, Contained CPPU and zeatin composition can activate cuttage cytoactive in growth accelerator A, promote branch cell division, and growth-promoting Heteroauxin and methyl α-naphthyl acetate composition can promote branch to root in long agent B, gibberellin improve cell splitting rate and nutrition into Divide and absorb.Hot-house culture method, nutrient solution culture is carried out after taking root, can further promote root growth, breeding, nutriment Absorb and leaf portion grows；Fermentation broth contents in nutrient solution, by the compositions such as peanut powder, pumpkin powder and black bamboo powder it is fermented after, wherein Protein to and fat etc. be degraded to small-molecule substance, and plant cell wall is destroyed, and improves nutritional ingredient in zymotic fluid The absorption rate of content and branch；And after the composition such as ginkgo leaf, Chinese pine leaf, common crapemyrtle leaf is extracted, contain in plant extraction liquid There are the organic principles such as B- sitosterol, stigmasterol, alkaloid, and abundant amino acid, trace element and vitamin content, can carry The resistance ability such as high cuttage branch disease and insect resistance, drought-resistant, and yellow pears fruit-growing time can be shortened, improve yield and yellow the operatic circle Real mouthfeel；And the irradiation of red blue light light filling is provided, red blue light illumination culture can promote cuttage branch to root growth, and magnetize and train The mode of supporting, hormone inside branch can be excited to produce, improve shoot maturation character, shorten plant fruit-growing time, and improve yield and Mouthfeel.Greenhouse method for transplanting, the cuttage branch through soil-less culture, its root is more fragile, and diseases and insect pests resistance is weaker, by Preculture is carried out in the soil of greenhouse planted area, cuttage branch root maturity, environment-adapting ability and diseases and insect pests resistance can be improved, Survival rate is planted so as to improve after outside soil is transplanted.
Embodiment
Embodiment 1：
A kind of method of yellow pear tree cuttage and seedling culture, comprises the following steps：
（1）Select branch：The age of tree is selected in 5-6 Huang pear tree branches, disease and insect resistance and environment adaptation ripe with physiological growth Ability is strong, it is possible to increase cuttage branch rooting rate and survival rate, a diameter of 1-2cm of branch, and intercepting process is carried out to branch, Obtain cuttage branch；
（2）Pretreatment：Cuttage branch bottom is wrapped up after cotton gauze is impregnated in bactericidal liquid, reduces microorganism to cuttage portion corruption Effect, and moisture is kept, and 5.5h is placed in 21 DEG C of isoperibols, it is subsequently placed in on-load voltage 37V, electric current 0.25A Ionization process 23min in ionization chamber, to cuttage bar cell-stimulating activity, improve growth rate；
（3）Dipping is taken root：Cuttage branch lower end after pretreatment is submerged initially in growth accelerator A, activates cuttage cytoactive, Promote branch cell division, 1.5h is soaked under 31 DEG C of bath temperatures, then soaks 3.5h in growth accelerator B, skewer can be promoted Rooting of cuttings germinates and nutrient absorption, and keeps above process chamber exhaust air rate to impregnate cuttage branch in 8.5L/min；
（4）Hot-house culture：It will impregnate in teat glass of the cuttage branch insertion equipped with nutrient solution, be placed on bed of nursing young plants in hothouses and carry out Culture, adjustment greenhouse temperature to 28 DEG C keep 2 days, humidity 67%, then by temperature adjust to 23 DEG C progress hot-house cultures, The red blue light illumination 3.5h/d of 420lux, and magnetize 21min per 4h using 0.35MHz magnetizers, obtain cuttage seeding；Contain in nutrient solution Abundant nutrition composition and active ingredient, it is possible to provide nutrition supply, and improve the speed of growth and shortening is born fruit the cycle, and magnetize training The mode of supporting, source electrode hormone in plant generation can be stimulated, improves shoot maturation character, shortens plant fruit-growing time, and improve yield；
（5）Transplant in greenhouse：Cuttage transplantation of seedlings through hot-house culture to greenhouse is planted in soil, planting depth 5-6cm, used Growth accelerator B is colonized, and every 5 days are poured 1 time using nutrient solution, and every usage amount is 45ml；Can improve cuttage branch root into Ripe degree, environment-adapting ability and diseases and insect pests resistance, so as to improve transplanting plantation survival rate.
Step（1）Described intercepting process, its intercepted length are 12cm, and branch upper end section keeps concordant, and retains 3-4 Piece spire, 30 ° of inclinations angle are cut into bottom, and draw 3-4 knives in notch flank vertical direction.
Step（2）Described bactericidal liquid, it is 800 times of dilute solutions of AVM.
Step（2）Described growth accelerator A, wherein CPPU:Zeatin quality proportioning is 2:1, its mass concentration is 0.32%；Described growth accelerator B, wherein heteroauxin：Naa：Gibberellin quality proportioning is 2:2:1, its mass concentration is 0.62%。
Step（4）Described nutrient solution, its compound method are：
51 parts of zymotic fluid, 22 parts of plant extraction liquid, 5.3 parts of growth accelerator B are weighed according to quality, culture uniformly is made after mixing Liquid.
Described zymotic fluid, its compound method are：
According to 106 parts of quality weighing water intaking, 13 parts of peanut powder, 11 parts of soybean juice, 7.2 parts of pumpkin powder, 4.3 parts of purple bamboo leaf powder, bitter sweet oak 3.2 parts of powder, 1.4 parts of hazelnut powder, the mix bacterium agent of water quality 2.3% is inoculated with after being stirred, is placed in 26 DEG C of fermentation tanks and cultivates 37h, zymotic fluid is made after 120 mesh filter screen filtrations.
Described plant extraction liquid, its compound method are：
18 parts of ginkgo leaf, 9 parts of grape juice, 7.4 parts of corn juice, 6 parts of common crapemyrtle leaf, 3.4 parts of Chinese pine leaf are weighed according to quality, first by silver Apricot leaf, common crapemyrtle leaf and Chinese pine leaf efflorescence processing, then the water of 3.5 times of gross mass is added after being mixed with grape juice and corn juice, it is placed in Boiling 1.5h at a temperature of 83 DEG C, plant extraction liquid is made after centrifugal filtration.
Embodiment 2：
Compared with embodiment 1, step changes in the following areas the present embodiment 2：
Step（1）Described intercepting process, its intercepted length are 13cm, and branch upper end section keeps concordant, and retains 3-4 pieces children Leaf, 30 ° of inclinations angle are cut into bottom, and draw 3-4 knives in notch flank vertical direction.
Step（2）Described ionization process, its parameter are：
On-load voltage 39V, electric current 0.35A, ionization time 28min in ionization chamber are immersed through cuttage bar.
Step（2）Described bactericidal liquid, it is 800 times of dilute solutions of AVM.
Step（2）Described growth accelerator A, wherein CPPU:Zeatin quality proportioning is 3:2, its mass concentration is 0.38%；Described growth accelerator B, wherein heteroauxin：Naa：Gibberellin quality proportioning is 3:2:1, its mass concentration is 0.76%。
Step（3）Dipping is taken root, and its method is：Soaked to cuttage bar is immersed in growth accelerator A under 33 DEG C of bath temperatures 2h is steeped, then soaks 4h in growth accelerator B is immersed.
Step（4）Described nutrient solution, its compound method are：
53 parts of zymotic fluid, 24 parts of plant extraction liquid, 5.8 parts of growth accelerator B are weighed according to quality, culture uniformly is made after mixing Liquid.
Described zymotic fluid, its compound method are：
According to 116 parts of quality weighing water intaking, 14 parts of peanut powder, 13 parts of soybean juice, 8.6 parts of pumpkin powder, 4.7 parts of purple bamboo leaf powder, bitter sweet oak 3.8 parts of powder, 1.8 parts of hazelnut powder, the mix bacterium agent of water quality 2.7% is inoculated with after being stirred, is placed in 28 DEG C of fermentation tanks and cultivates 38h, zymotic fluid is made after 120 mesh filter screen filtrations.
Described plant extraction liquid, its compound method are：
22 parts of ginkgo leaf, 11 parts of grape juice, 8.7 parts of corn juice, 7 parts of common crapemyrtle leaf, 4.6 parts of Chinese pine leaf are weighed according to quality, first will Ginkgo leaf, common crapemyrtle leaf and Chinese pine leaf efflorescence processing, then the water of 4 times of gross mass is added after being mixed with grape juice and corn juice, it is placed in Boiling 2h at a temperature of 86 DEG C, plant extraction liquid is made after centrifugal filtration.
Step（4）Hot-house culture, its method are：
It will impregnate in teat glass of the cuttage branch insertion equipped with nutrient solution, be placed on bed of nursing young plants in hothouses and cultivated, adjustment temperature Room temperature to 31 DEG C keep 3 days, humidity 68%, then by temperature adjust to 24 DEG C progress hot-house cultures, the red blue light illuminations of 430lux 4h/d, and magnetize 22min per 5h using 0.46MHz magnetizers.
Contrast 1：
Step is not used compared with embodiment 1 in this contrast 1（1）Middle 5-6 Huangs pear tree branch, other steps and the phase of embodiment 1 Together.
Contrast 2：
This contrast 2 does not carry out step compared with embodiment 1（2）Ionization process, other steps are same as Example 1.
Contrast 3：
This contrast 3 does not carry out step compared with embodiment 1（3）Dipping is taken root, and other steps are same as Example 1.
Contrast 4：
This contrast 4 does not carry out step compared with embodiment 2（4）In red blue light illumination, other steps are same as Example 2.
Contrast 5：
This contrast 5 does not carry out step compared with embodiment 2（4）Middle magnetizer uses, and other steps are same as Example 2.
Contrast 6：
This contrast 5 does not carry out step compared with embodiment 2（5）Greenhouse is transplanted, and other steps are same as Example 2.
Control group：
Control group is directly planted after being soaked to yellow pears cuttage bar root-inducing powder, and 5-6 Huang pear trees branch, ionization process, leaching is not used Stain is taken root, red blue light illumination, magnetizer and greenhouse method for transplanting.
To embodiment 1, embodiment 2, contrast 1, contrast 2, contrast 3, contrast 4, contrast 5, contrast 6 and control group experiment side Case, count the Rooting percent of yellow pears cuttage seeding, survival rate, single yield（2nd year）, sugar content is compared.
Experimental data：
Project Rooting percent % Survival rate % Single yield（2nd year）kg Sugar content %
Embodiment 1 96.34% 94.57% 9.78 17.46%
Embodiment 2 95.89% 94.13% 9.53 17.27%
Contrast 1 94.57% 91.77% 7.55 17.42%
Contrast 2 93.63% 92.97% 9.78 16.62%
Contrast 3 92.33% 92.57% 8.23 17.46%
Contrast 4 92.94% 90.93% 8.85 16.10%
Contrast 5 92.23% 89.47% 6.53 15.38%
Contrast 6 95.78% 90.27% 7.21 17.21%
Control group 81.24% 76.45% 0 13.56%
Synthesis result：The yellow pear tree that the inventive method is cultivated, compared with control group, its rooting rate improves 15.10%, and it is survived Rate improves 18.12%, and the 2nd year single yield is more than 9.53kg, and sugar content improves 3.90%.Use 5-6 branches and ionization Method, it is respectively 1.77%, 2.71% that can improve Rooting percent, and survival rate improves 2.80%, 1.60%；, can using dipping rooting method It is 4.01% to improve Rooting percent, and survival rate improves 2.0%, single output increased 1.55kg；And use red blue light illumination and magnetizer Method, it is 2.95%, 3.66% that can improve rooting rate, and survival rate improves 3.2%, 4.66%, and sugar content improves 1.17%, 1.89%； And hot-house culture method is used, survival rate 3.86%, single output increased 2.32kg can be improved.

Claims (7)

  1. A kind of 1. method of yellow pear tree cuttage and seedling culture, it is characterised in that comprise the following steps：
    （1）Select branch：The age of tree is selected in 5-6 Huang pear tree branches, a diameter of 1-2cm of branch, and branch is carried out at interception Reason, obtains cuttage branch；
    （2）Pretreatment：Cuttage branch bottom is wrapped up after cotton gauze is impregnated in bactericidal liquid, and in 20-24 DEG C of isoperibol 5-6h is placed, is subsequently placed in ionization process 20-30min in on-load voltage 36-40V, electric current 0.2-0.4A ionization chambers；
    （3）Dipping is taken root：Cuttage branch after pretreatment is submerged initially in growth accelerator A, soaked under 30-34 DEG C of bath temperature 1-2h is steeped, 3-4h is then soaked in growth accelerator B, and keeps above process chamber exhaust air rate to obtain dipping cuttage in 8-10L/min Branch；
    （4）Hot-house culture：It will impregnate in teat glass of the cuttage branch insertion equipped with nutrient solution, be placed on bed of nursing young plants in hothouses and carry out Culture, adjustment greenhouse temperature to 27-32 DEG C of holding 2-3 days, humidity 65%-70%, then temperature is adjusted to 22-25 DEG C carry out temperature Room is cultivated, the red blue light illumination 3-4h/d of 410-440lux, and magnetizes 20-23min per 4-5h using 0.3-0.5MHz magnetizers, is obtained Cuttage seeding；
    （5）Transplant in greenhouse：Cuttage transplantation of seedlings through hot-house culture to greenhouse is planted in soil, planting depth 5-6cm, used Growth accelerator B is colonized, and is poured 1 time using nutrient solution per 5-6 days, and every usage amount is 40-60ml.
  2. 2. the method for yellow pear tree cuttage and seedling culture as claimed in claim 1, it is characterised in that step（1）Described intercepting process, its Intercepted length is 10-14cm, and branch upper end section keeps concordant, and retains 3-4 piece spires, and 30 ° of inclinations angle are cut into bottom, and Notch flank vertical direction draws 3-4 knives.
  3. 3. the method for yellow pear tree cuttage and seedling culture as claimed in claim 1, it is characterised in that step（2）Described bactericidal liquid, for Ah Tie up 800 times of dilute solutions of rhzomorph.
  4. 4. the method for yellow pear tree cuttage and seedling culture as claimed in claim 1, it is characterised in that step（2）Described growth accelerator A, Wherein CPPU:Zeatin quality proportioning is 2-3:1-2, its mass concentration are 0.3%-0.4%；Described growth accelerator B, wherein Yin Indolylbutyric acid：Naa：Gibberellin quality proportioning is 2-3:2:1, its mass concentration is 0.6%-0.8%.
  5. 5. the method for yellow pear tree cuttage and seedling culture as claimed in claim 1, it is characterised in that step（4）Described nutrient solution, it is matched somebody with somebody Method processed is：
    Zymotic fluid 50-54 parts, plant extraction liquid 21-25 parts, growth accelerator B 5-6 parts are weighed according to quality, is uniformly made after mixing Obtain nutrient solution.
  6. 6. the method for yellow pear tree cuttage and seedling culture as claimed in claim 5, it is characterised in that described zymotic fluid, its compound method For：
    According to quality weighing water intaking 100-120 parts, peanut powder 12-15 parts, soybean juice 10-14 parts, pumpkin powder 7-9 parts, purple bamboo leaf powder 4-5 parts, bitter sweet oak powder 3-4 parts, hazelnut powder 1-2 parts, water quality 2%-3% mix bacterium agent is inoculated with after being stirred, is placed in 25-29 DEG C 36-40h is cultivated in fermentation tank, zymotic fluid is made after 120 mesh filter screen filtrations.
  7. 7. the method for yellow pear tree cuttage and seedling culture as claimed in claim 5, it is characterised in that described plant extraction liquid, it is prepared Method is：
    Ginkgo leaf 17-23 parts, grape juice 8-12 parts, corn juice 7-9 parts, common crapemyrtle leaf 5-8 parts, Chinese pine leaf 3-5 are weighed according to quality Part, first ginkgo leaf, common crapemyrtle leaf and the efflorescence of Chinese pine leaf are handled, then 3-4 times of gross mass is added after being mixed with grape juice and corn juice Water, boiling 1-2h at a temperature of being placed in 82-87 DEG C, after centrifugal filtration be made plant extraction liquid.
CN201710748132.6A 2017-08-28 2017-08-28 A kind of method of yellow pear tree cuttage and seedling culture Pending CN107409921A (en)

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CN104584849A (en) * 2015-02-06 2015-05-06 青岛华盛绿能农业科技有限公司 Photinia serrulata cuttage seedling culture method
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Application publication date: 20171201