CN107406892A - 捕获精子核酸的方法 - Google Patents
捕获精子核酸的方法 Download PDFInfo
- Publication number
- CN107406892A CN107406892A CN201680019880.4A CN201680019880A CN107406892A CN 107406892 A CN107406892 A CN 107406892A CN 201680019880 A CN201680019880 A CN 201680019880A CN 107406892 A CN107406892 A CN 107406892A
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- dna
- antibody
- nucleoprotamine
- spermatoblast
- protamin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
- C12Q2522/10—Nucleic acid binding proteins
- C12Q2522/101—Single or double stranded nucleic acid binding proteins
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- Pregnancy & Childbirth (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Gynecology & Obstetrics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (45)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/672358 | 2015-03-30 | ||
US14/672,358 US9957548B2 (en) | 2015-03-30 | 2015-03-30 | Methods of capturing sperm nucleic acids |
PCT/EP2016/056854 WO2016156353A1 (en) | 2015-03-30 | 2016-03-30 | Methods of capturing sperm nucleic acids |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107406892A true CN107406892A (zh) | 2017-11-28 |
CN107406892B CN107406892B (zh) | 2022-05-13 |
Family
ID=55637383
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201680019880.4A Active CN107406892B (zh) | 2015-03-30 | 2016-03-30 | 捕获精子核酸的方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US9957548B2 (zh) |
EP (1) | EP3277834B1 (zh) |
JP (1) | JP6758315B2 (zh) |
CN (1) | CN107406892B (zh) |
WO (1) | WO2016156353A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114088491A (zh) * | 2021-11-24 | 2022-02-25 | 北京仁基源医学研究院有限公司 | 一种精子dna碎片检测试剂盒及其检测方法 |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10030241B2 (en) | 2015-03-30 | 2018-07-24 | General Electric Company | Methods and kits for capturing sperm nucleic acids |
CN110352243B (zh) * | 2017-03-27 | 2024-05-28 | 积水医疗株式会社 | 使用抗体的靶标核酸浓缩和回收方法 |
CN107058547A (zh) * | 2017-04-28 | 2017-08-18 | 首度生物科技(苏州)有限公司 | 一种精子的检测方法 |
TWI775252B (zh) | 2020-12-23 | 2022-08-21 | 邦睿生技股份有限公司 | 用於偵測精子dna片段化的方法以及套組 |
US11298375B2 (en) * | 2021-10-12 | 2022-04-12 | Terry Earl Brady | Halogenated fullerene functionalized as a biocidal and chemotactic spermicide to vaginally harbor and neutralize spermatozoa for use as a safe and effective contraceptive |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020197637A1 (en) * | 2001-06-02 | 2002-12-26 | Willson Richard C. | Process and compositions for protection of nucleic acids |
WO2005079243A2 (en) * | 2004-02-06 | 2005-09-01 | University Of Virginia Patent Foundation | Compositions and methods for identifying sperm for forensic applications |
CN101134772A (zh) * | 2007-07-25 | 2008-03-05 | 中国农业大学 | 一种哺乳类精子头后部膜蛋白的分离方法及其应用 |
WO2008101192A1 (en) * | 2007-02-16 | 2008-08-21 | Applied Biosystems, Llc | Method for recovering nucleic acid from a mixed cell suspension, without centrifugation |
CN102296062A (zh) * | 2011-08-29 | 2011-12-28 | 中国农业大学 | 一种公牛冻精基因组dna的提取方法 |
CN102812118A (zh) * | 2010-04-08 | 2012-12-05 | 恰根有限公司 | 分离和纯化核酸的方法 |
Family Cites Families (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4444879A (en) | 1981-01-29 | 1984-04-24 | Science Research Center, Inc. | Immunoassay with article having support film and immunological counterpart of analyte |
US5436157A (en) | 1989-03-03 | 1995-07-25 | The University Of Virginia Alumni Patents Foundation | Human intra-acrosomal sperm antigen |
US5602005A (en) | 1989-03-03 | 1997-02-11 | The University Of Virginia Alumni Patents Foundation | Primate intra-acrosomal sperm antigen for use in a contraceptive vaccine |
US5075078A (en) | 1989-10-05 | 1991-12-24 | Abbott Laboratories | Self-performing immunochromatographic device |
US5721348A (en) | 1990-12-14 | 1998-02-24 | University Of Connecticut | DNA encoding PH-20 proteins |
US5712172A (en) | 1995-05-18 | 1998-01-27 | Wyntek Diagnostics, Inc. | One step immunochromatographic device and method of use |
KR100292182B1 (ko) | 1997-09-18 | 2001-11-26 | 모리시타 요이찌 | 면역크로마토그라피장치 |
AU1778701A (en) | 1999-11-17 | 2001-05-30 | University Of Virginia Patent Foundation | Sperm cell selection system |
US7842783B2 (en) | 2002-02-14 | 2010-11-30 | Japan Science And Technology Agency | Mouse spermatogenesis genes related to human male sterility |
EP1542529A4 (en) | 2002-07-22 | 2006-08-09 | Xy Inc | SYSTEM FOR TREATING SPERMATIC CELL |
US7153687B2 (en) | 2002-08-13 | 2006-12-26 | Hong Kong Dna Chips Limited | Apparatus and methods for detecting DNA in biological samples |
US20050032097A1 (en) | 2003-06-17 | 2005-02-10 | Garvin Alex M. | Method for processing samples containing sperm and non-sperm cells for subsequent analysis of the sperm DNA |
US7320891B2 (en) | 2004-09-10 | 2008-01-22 | Promega Corporation | Methods and kits for isolating sperm cells |
US20070141583A1 (en) | 2005-12-20 | 2007-06-21 | Weiwei Li | Methods of rapid chromatin immunoprecipitation |
BRPI0718231A2 (pt) | 2006-10-06 | 2013-11-12 | Promega Corp | Métodos e estojos para isolar células |
US8521440B2 (en) | 2007-01-08 | 2013-08-27 | The Invention Science Fund I, Llc | Systems for genome selection |
US11781127B2 (en) | 2007-01-16 | 2023-10-10 | Alex Garvin | Homogeneous method to prepare sperm DNA from sexual assault cases |
WO2008089280A2 (en) | 2007-01-16 | 2008-07-24 | Applied Biosystems, Llc | Selective lysis of sperm cells |
EP2171058A1 (en) | 2007-07-23 | 2010-04-07 | Applied Biosystems Inc. | Method for recovering sperm nucleic acid from a forensic sample |
CN101795744A (zh) | 2007-09-11 | 2010-08-04 | 阿尔利克斯公司 | 用于分选物体的结合方法和仪器 |
US7998676B2 (en) | 2007-09-13 | 2011-08-16 | Arryx, Inc. | Methods and apparatuses for sorting objects in forensic DNA analysis and medical diagnostics |
US20090111185A1 (en) | 2007-10-26 | 2009-04-30 | Searete Llc, A Limited Liability Corporation Of The State Of Delaware | Female genome selection |
EP2100969A1 (en) | 2008-03-14 | 2009-09-16 | Qiagen GmbH | Rapid method for identifying polypeptide-nucleic acid interactions |
JP5117928B2 (ja) | 2008-05-27 | 2013-01-16 | 富士フイルム株式会社 | イムノクロマトグラフデバイス |
WO2010120518A2 (en) | 2009-04-01 | 2010-10-21 | Biocern, Inc. | Sperm cell separation methods and compositions containing sperm cell targeting ligands for use therein |
EP2576779B1 (en) | 2010-06-01 | 2017-08-09 | Qiagen GmbH | Method for isolating and/or purifying nucleic acid(s) |
WO2012033714A2 (en) | 2010-09-07 | 2012-03-15 | Sigma-Aldrich Co., Llc | Cells for chromatin immunoprecipitation and methods for making |
WO2012047726A1 (en) | 2010-09-29 | 2012-04-12 | The Broad Institute, Inc. | Methods for chromatin immuno-precipitations |
GB201207788D0 (en) | 2012-05-03 | 2012-06-13 | Univ Newcastle | Methods relating to identification of susceptibility to liver injury |
JP2015528293A (ja) | 2012-08-29 | 2015-09-28 | イングラン, エルエルシー | 損傷または欠陥のある細胞または細胞組織の磁気的除去または識別 |
-
2015
- 2015-03-30 US US14/672,358 patent/US9957548B2/en active Active
-
2016
- 2016-03-30 WO PCT/EP2016/056854 patent/WO2016156353A1/en active Application Filing
- 2016-03-30 EP EP16712051.8A patent/EP3277834B1/en active Active
- 2016-03-30 JP JP2017549091A patent/JP6758315B2/ja active Active
- 2016-03-30 CN CN201680019880.4A patent/CN107406892B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020197637A1 (en) * | 2001-06-02 | 2002-12-26 | Willson Richard C. | Process and compositions for protection of nucleic acids |
WO2005079243A2 (en) * | 2004-02-06 | 2005-09-01 | University Of Virginia Patent Foundation | Compositions and methods for identifying sperm for forensic applications |
WO2008101192A1 (en) * | 2007-02-16 | 2008-08-21 | Applied Biosystems, Llc | Method for recovering nucleic acid from a mixed cell suspension, without centrifugation |
CN101134772A (zh) * | 2007-07-25 | 2008-03-05 | 中国农业大学 | 一种哺乳类精子头后部膜蛋白的分离方法及其应用 |
CN102812118A (zh) * | 2010-04-08 | 2012-12-05 | 恰根有限公司 | 分离和纯化核酸的方法 |
CN102296062A (zh) * | 2011-08-29 | 2011-12-28 | 中国农业大学 | 一种公牛冻精基因组dna的提取方法 |
Non-Patent Citations (6)
Title |
---|
FRANCESCO DATI等: "《蛋白质实验室检测项目临床应用指南》", 31 October 2008, 上海科学技术出版社 * |
ROD BALHORN等: ""The protamine family of sperm nuclear proteins"", 《GENOME BIOL》 * |
于源华等: "《生物工程与技术专业实验教程》", 30 September 2009, 兵器工业出版社 * |
吴明章等: "《生殖疾病诊断学》", 30 November 2006, 上海科学出版社 * |
李鑫等: ""混合斑中精子细胞分离及其DNA 制备方法"", 《法医学杂志》 * |
赵兴春等: ""精子细胞定向捕获与分离技术初步研究"", 《刑事技术》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114088491A (zh) * | 2021-11-24 | 2022-02-25 | 北京仁基源医学研究院有限公司 | 一种精子dna碎片检测试剂盒及其检测方法 |
Also Published As
Publication number | Publication date |
---|---|
JP2018511313A (ja) | 2018-04-26 |
EP3277834B1 (en) | 2021-02-17 |
US9957548B2 (en) | 2018-05-01 |
EP3277834A1 (en) | 2018-02-07 |
WO2016156353A1 (en) | 2016-10-06 |
JP6758315B2 (ja) | 2020-09-23 |
US20160289735A1 (en) | 2016-10-06 |
CN107406892B (zh) | 2022-05-13 |
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CB02 | Change of applicant information |
Address after: Great Britain Applicant after: Kaijie medical biotechnology System Co.,Ltd. Address before: Great Britain Applicant before: GE Medical Biotechnology Systems Co.,Ltd. |
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TA01 | Transfer of patent application right |
Effective date of registration: 20191108 Address after: Great Britain Applicant after: GE Medical Biotechnology Systems Co.,Ltd. Address before: New York State, USA Applicant before: General Electric Co. |
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Effective date of registration: 20210106 Address after: German Heerden Applicant after: Kaijie medical biotechnology System Co.,Ltd. Address before: Great Britain Applicant before: Kaijie medical biotechnology System Co.,Ltd. |
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