CN107375628A - A kind of micro-capsule loads plant source fish insect killing agent - Google Patents
A kind of micro-capsule loads plant source fish insect killing agent Download PDFInfo
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- CN107375628A CN107375628A CN201710508089.6A CN201710508089A CN107375628A CN 107375628 A CN107375628 A CN 107375628A CN 201710508089 A CN201710508089 A CN 201710508089A CN 107375628 A CN107375628 A CN 107375628A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/24—Apocynaceae (Dogbane family), e.g. plumeria or periwinkle
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/483—Gleditsia (locust)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/78—Saururaceae (Lizard's-tail family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5036—Polysaccharides, e.g. gums, alginate; Cyclodextrin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The present invention relates to technical field of insecticide, plant source fish insect killing agent is loaded more particularly to a kind of micro-capsule, bulk drug is mainly made up of the plants such as onion, oleander and gleditsia sinensis and Chinese herbal medicine, and the preparation method of target product includes the step such as the extraction of each bulk drug active component and the preparation of cyclodextrin succinate micro-capsule；Different material medicine component is extracted using different method and solvent, it is ensured that the content of active component；The trace meter that a variety of functional groups such as the hydroxyl of alkaloid, ester group mutually promote in activation, with parasite body in extract, which forms a variety of chelates, causes the death of parasite；Onion and cordate houttuynia promote the group activations such as the α glycosidic bonds of astragalus polyose, not only kill parasite, and the hepatic injury to fish has protective effect；Beta cyclodextrin succinate micro-capsule carrying active ingredients, insecticide is reached the receptor site of parasite and precisely discharge, avoid dosage from using excessive, reduce the frequency of use of insecticide, reduce cost.
Description
Technical field
The present invention relates to technical field of insecticide, and in particular to a kind of micro-capsule loads plant source fish insect killing agent.
Background technology
Along with developing rapidly for China's culture fishery, the species and frequency of disease development of aquatic products disease also increase sharply, closely
Nian Lai, because the harm of eliminating fish parasites disease is on the rise, the extensive application of chemical synthetic drug, cause the resistance to the action of a drug, safety
Property and the destruction sex chromosome mosaicism to breeding ecological environment become increasingly conspicuous, and cause appliable plant insecticide cry increasingly
It is high.Meanwhile the raising with people's quality of life and the concern to ecological environment, the main flow that nuisanceless aquatic products turn into people need
Ask, the nuisanceless quality standard of the aquatic products especially exported is higher, and plant insecticide is the nuisanceless aquatic products of production
Important guarantee, possess wide domestic and international market.It is micro- that Chinese patent CN1250086C discloses a kind of nimbin compound plant source
Emulsion insecticide and preparation method thereof and the purposes in aquaculture, insecticidal effect are better than single nimbin, expand print
The insecticidal spectrum of chinaberry element, insecticidal activity is enhanced, but in subacute toxicity test, arecaline and matrine that the invention includes
There is damaging action to the internal organs of fish.Chinese patent CN104542401B discloses a kind of ecological cultivation method of grass carp, to
Chinese tallow tree, masson pine, margosa, the fresh and tender branches and leaves of non-irrigated peppery and knotweed are launched in culturing pool, and coordinate Chinese medicine health-care pharmaceutical chemistry to carry out pest and disease damage
Preventing and treating, have chosen plurality of Chinese kind, not only increases the cumbersome of process for preparation, and add cost.So this area
Technical staff is still making great efforts green formula of the research for the aquaculture prevention and control of plant diseases, pest control, to reach the same of more preferable insecticidal effect
When, improve the security to non-target organism and cultivation pool environment.
Exploitation to plant insecticide gradually rises to the science stage by experience, uses for reference grinding for China's plant insecticide
Study carefully experience, the plant insecticide that eliminating fish parasites are killed in exploitation is both the needs in market, is that China's culture fishery walks can again
The needs of sustainable development strategy, and there is real necessity.
The content of the invention
In view of the shortcomings of the prior art, the invention provides a kind of micro-capsule to load plant source fish insect killing agent, from source
Extensive plant and Chinese herbal medicine are that raw material extracts active component, using beta-schardinger dextrin-succinate micro-capsule carrying active ingredients, are made
Its milligram ammonia discharges, and effectively kills parasite.
To realize object above, the present invention is achieved by the following technical programs：
A kind of micro-capsule loads plant source fish insect killing agent, including active component and micro-capsule, the active component by containing with
The bulk drug extraction gained of lower weight percentage component：Onion 28-30%, oleander 20-23%, gleditsia sinensis 10-12%, Chinese cassia tree
8-10%, honeysuckle 2-2.5%, short-tube lycoris 1-2%, Radix Astragali 1-2%, cordate houttuynia 0.5-1%, remaining is rice husk；The micro-capsule by
Beta-schardinger dextrin and succinic anhydride are prepared.
Further, the bulk drug contains the component of following weight percentage：Onion 28-29%, oleander 21-
22%th, gleditsia sinensis 11-12%, Chinese cassia tree 9-10%, honeysuckle 2.2-2.5%, short-tube lycoris 1.5-2%, Radix Astragali 1-1.5%, cordate houttuynia
0.8-1%, remaining is rice husk.
Further, the oleander is selected from oleander fresh leaf and stem skin.
Further, preparation method comprises the following steps：
(1) extraction of active component：Weigh bulk drug according to weight percentage ratio, crush, by oleander, gleditsia sinensis and
Short-tube lycoris is immersed in chloroform, 55-60 DEG C of ultrasonic extraction 0.5h, extracts 3 times, merges extract solution, be concentrated under reduced pressure to obtain medicinal extract 1, by the Radix Astragali
It is immersed in cordate houttuynia in 80% ethanol solution, reserved filtrate is filtered after microwave abstracting 0.5h, filter residue adds after being concentrated under reduced pressure is steamed
Distilled water, filter after microwave abstracting 0.5h, after merging concentration filtrate, add absolute ethyl alcohol to adjust alcoholic degree to 80%, stand 8-12h, surpass
Speed centrifuges to obtain sediment, and remaining bulk drug is immersed in 75% ethanol solution, refluxing extraction 1-2h, extracted 3 times, merging carries
Liquid is taken, be concentrated under reduced pressure to obtain medicinal extract 2；
(2) preparation of micro-capsule：Beta-schardinger dextrin is dissolved in 60 DEG C of hot water, succinic anhydride is added, 90 DEG C of reaction 0.5h of microwave, adds
Enter weakly alkaline solution, chloroform extraction, concentrate organic phase, ether precipitating produces beta-schardinger dextrin-succinate micro-capsule；
(3) preparation of insecticide：The medicinal extract 1, sediment and medicinal extract 2 of step (1) are dissolved in chloroform, add step (2)
Beta-schardinger dextrin-succinate micro-capsule, after reacting at room temperature 10-12h, concentration, ether precipitating, filtration drying obtains insecticide.
Further, the weakly alkaline solution is selected from saturated sodium bicarbonate solution, saturated sodium carbonate solution or 0.2mol
L-1Sodium hydroxide solution, preferably saturated sodium bicarbonate solution.
Further, the power of the microwave abstracting is 350W.
Due to using above-mentioned technical scheme, of the invention is beneficial as follows：
The present invention is extracted using different method and solvent to different material medicine component, it is ensured that in the extract of gained
Active component content highest；A variety of functional groups such as the hydroxyl of alkaloid, ester group in the chloroform extract of oleander, gleditsia sinensis and short-tube lycoris
The trace meter in activation, with parasite body of mutually promoting forms a variety of chelates so as to cause the death of parasite, kills in vitro
Worm experiment shows that the insecticidal activity of insecticide of the present invention is better than nimbin raw medicine, especially 96h insecticide of the present invention to parasite
Killing rate such as Dactylogyrus and Trichodina reaches 100%；Onion and cordate houttuynia promote α-sugar of astragalus polyose in Astragalus Root P.E
The group activations such as glycosidic bond, not only combined with the enzyme in parasite body and kill parasite, and the hepatic injury to fish has protection
And anti-oxidative stress；Beta configuration activation oleander, Chinese cassia tree, honeysuckle and the cordate houttuynia of cardiac glycoside carry in oleander extract
Aldehyde radical that thing includes and the noncompetitive hydrophobic lipid material with saprolegnia hypha cell membrane of unsaturated double-bond is taken to be combined, it is effectively broken
The structure of bad cell wall and cell membrane, make its plasmolysis, accelerate the destruction of each organelle of intracellular.
Esterification occurs in the presence of microwave for the hydroxyl of small molecule polycondensation monomer succinic anhydride and beta-schardinger dextrin, generation
Beta-schardinger dextrin-succinate, the ester bond of beta-schardinger dextrin-succinate are cross-linked with each other to form micro-capsule, produced around active component
Physical barrier, active component is carried on micro-capsule inner space, after insecticide reaches the receptor site of parasite, in relevant enzyme
The lower ester linkage breaking discharge active component of effect, realizes the accurate release of active component, avoids dosage from using excessive, to aquatic products
Quality has an impact, while reduces the frequency of use of insecticide, reduces cost.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention,
Technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is the present invention one
Divide embodiment, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making
The every other embodiment obtained under the premise of creative work, belongs to the scope of protection of the invention.
Embodiment 1：
A kind of micro-capsule loads plant source fish insect killing agent, including active component and micro-capsule, and active component is by containing following heavy
Measure the bulk drug extraction gained of percentage composition component：Onion 28%, oleander 20%, gleditsia sinensis 10%, Chinese cassia tree 8%, honeysuckle
2%th, short-tube lycoris 1%, the Radix Astragali 1%, cordate houttuynia 0.5%, remaining is rice husk 29.5%, and the micro-capsule is by beta-schardinger dextrin and succinic anhydride
It is prepared.Preparation method comprises the following steps：
(1) extraction of active component：Bulk drug 100g is weighed according to weight percentage ratio, respectively by bulk drug each group
Divide and crush, the oleander 20g, gleditsia sinensis 10g and short-tube lycoris 1g of crushing are immersed in 200ml chloroforms, 55 DEG C of ultrasonic extraction 0.5h, extraction
3 times, merge extract solution, be concentrated under reduced pressure to obtain medicinal extract, and Radix Astragali 1g and cordate houttuynia 0.5g are immersed in 10ml80% ethanol solution,
Regulation microwave power is 350W, and reserved filtrate is filtered after extracting 0.5h, and 15ml distilled water, microwave power are added into the filter residue of concentration
Still it is 350W, is filtered after extracting 0.5h, merge the filtrate of 2 times, after concentration, adds absolute ethyl alcohol to adjust alcoholic degree to 80%, stand 12h
Afterwards, ultracentrifugation obtains sediment, and remaining bulk drug onion 28g, Chinese cassia tree 8g, honeysuckle 2g and rice husk 29.5g are immersed in
500ml75% ethanol solution, refluxing extraction 2h, extract 3 times, merge extract solution, be concentrated under reduced pressure to obtain medicinal extract；
(2) preparation of beta-schardinger dextrin-succinate：Beta-schardinger dextrin is dissolved in 60 DEG C of hot water, adds succinic anhydride microwave 90
DEG C reaction 0.5h, adds saturated sodium bicarbonate solution and is sufficiently stirred 10min, and chloroform extracts 3 times, merges organic phase, after concentration plus
Enter ether precipitating and obtain beta-schardinger dextrin-succinate；
(3) preparation of insecticide：The medicinal extract 1, sediment and medicinal extract 2 of step (1) are dissolved in chloroform, add step (2)
Beta-schardinger dextrin-succinate micro-capsule, after reacting at room temperature 10h, chloroform is concentrated, adds ether precipitating, filtration drying obtains insecticide.
Embodiment 2：
A kind of micro-capsule loads plant source fish insect killing agent, including active component and micro-capsule, and active component is by containing following heavy
Measure the bulk drug extraction gained of percentage composition component：Onion 30%, oleander 23%, gleditsia sinensis 12%, Chinese cassia tree 10%, honeysuckle
2.5%th, short-tube lycoris 2%, the Radix Astragali 2%, cordate houttuynia 1%, remaining is rice husk 17.5g；The micro-capsule is by beta-schardinger dextrin and succinic anhydride
It is prepared.Preparation method comprises the following steps：
(1) extraction of active component：Bulk drug 100g is weighed according to weight percentage ratio, respectively by bulk drug each group
Divide and crush, the oleander 23g, gleditsia sinensis 12g and short-tube lycoris 2g of crushing are immersed in 250ml chloroforms, 60 DEG C of ultrasonic extraction 0.5h, extraction
3 times, merge extract solution, be concentrated under reduced pressure to obtain medicinal extract 1, and Radix Astragali 2g and cordate houttuynia 1g are immersed in 20ml80% ethanol solution,
Regulation microwave power is 350W, filters reserved filtrate after extracting 0.5h, filter residue adds 30ml distilled water, microwave power after being concentrated under reduced pressure
Still it is 350W, is filtered after extracting 0.5h, merge the filtrate of 2 times, after concentration, adds absolute ethyl alcohol to adjust alcoholic degree to 80%, stand 12h
Afterwards, ultracentrifugation obtains sediment, and remaining bulk drug onion 30g, Chinese cassia tree 10g, honeysuckle 2.5g and rice husk 17.5g are immersed in
600ml75% ethanol solution, refluxing extraction 2h, extract 3 times, merge extract solution, be concentrated under reduced pressure to obtain medicinal extract 2；
(2) preparation of micro-capsule：Beta-schardinger dextrin is dissolved in 60 DEG C of hot water, 90 DEG C of reaction 0.5h of succinic anhydride microwave is added, adds
Enter saturated sodium bicarbonate solution and be sufficiently stirred 10min, chloroform extracts 3 times, merges organic phase, added after concentration ether precipitating obtain β-
Cyclodextrin-succinate micro-capsule；
(3) preparation of insecticide：The medicinal extract 1, sediment and medicinal extract 2 of step (1) are dissolved in chloroform, add step (2)
Beta-schardinger dextrin-succinate micro-capsule, after reacting at room temperature 12h, chloroform is concentrated, adds ether precipitating, filtration drying obtains insecticide.
Embodiment 3：
A kind of micro-capsule loads plant source fish insect killing agent, including active component and micro-capsule, and active component is by containing following heavy
Measure the bulk drug extraction gained of percentage composition component：Onion 30%, oleander 22%, gleditsia sinensis 12%, Chinese cassia tree 8%, honeysuckle
2%th, short-tube lycoris 1.5%, the Radix Astragali 2%, cordate houttuynia 1%, remaining is rice husk 21.5%；The micro-capsule is by beta-schardinger dextrin and succinic anhydride
It is prepared.Preparation method comprises the following steps：
(1) extraction of active component：Bulk drug 100g is weighed according to weight percentage ratio, respectively by bulk drug each group
Divide and crush, the oleander 22g, gleditsia sinensis 12g and short-tube lycoris 1.5g of crushing are immersed in 250ml chloroforms, 60 DEG C of ultrasonic extraction 0.5h, carried
Take 3 times, merge extract solution, be concentrated under reduced pressure to obtain medicinal extract 1, and Radix Astragali 2g and cordate houttuynia 1g are immersed in 20ml80% ethanol solution
In, regulation microwave power is 350W, and reserved filtrate is filtered after extracting 0.5h, and 30ml distilled water, microwave are added into the filter residue of concentration
Power is still 350W, is filtered after extracting 0.5h, merges the filtrate of 2 times, after concentration, adds absolute ethyl alcohol to adjust alcoholic degree to 80%, quiet
After putting 12h, ultracentrifugation obtains sediment, and remaining bulk drug onion 30g, Chinese cassia tree 8g, honeysuckle 2g and rice husk 21.5g are submerged
In 600ml75% ethanol solution, refluxing extraction 2h, extract 3 times, merge extract solution, be concentrated under reduced pressure to obtain medicinal extract 2；
(2) preparation of micro-capsule：Beta-schardinger dextrin is dissolved in 60 DEG C of hot water, 90 DEG C of reaction 0.5h of succinic anhydride microwave is added, adds
Enter saturated sodium bicarbonate solution and be sufficiently stirred 10min, chloroform extracts 3 times, merges organic phase, added after concentration ether precipitating obtain β-
Cyclodextrin-succinate micro-capsule；
(3) preparation of insecticide：The medicinal extract 1, sediment and medicinal extract 2 of step (1) are dissolved in chloroform, add step (2)
Beta-schardinger dextrin-succinate micro-capsule, after reacting at room temperature 12h, chloroform is concentrated, adds ether precipitating, filtration drying obtains insecticide.
External insecticidal test, using nimbin raw medicine as positive control, the insecticide of embodiment 1-3 preparations is obtained when different
Between section to parasite Dactylogyrus and the killing rate of Trichodina, as a result as shown in table 1.
The nimbin raw medicine of table 1 and embodiment 1-3 are to Dactylogyrus and the killing rate of Trichodina
As shown in Table 1, insecticide of the invention is above printing different time is short to the killing rate of Dactylogyrus and Trichodina
Chinaberry element active compound, 96h embodiments 1-3 killing rate reaches 100%, and positive control still has parasite survival.
With carbon tetrachloride (CCl4) induce the hepatic injury of carp to establish external acute hepatic injury model, intracellular solubility
Enzyme GPT, GOT and LDH spill-out significantly increases, and these three enzymes are the classical indexs whether liver cell damages.CCl4Liver cell damages
After wound, membrane passage increase, cause GPT, GOT and LDH to be released into cell culture fluid and serum, cell culture fluid and
GPT, GOT and LDH concentration significantly raise in serum.The present invention insecticide can pole significantly inhibit by CCl4Cause in liver cell
GPT, GOT and LDH activity rise, there is protective effect to liver.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply and deposited between these entities or operation
In any this actual relation or order.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Nonexcludability includes, so that process, method, article or equipment including a series of elements not only will including those
Element, but also the other element including being not expressly set out, or it is this process, method, article or equipment also to include
Intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that
Other identical element also be present in process, method, article or equipment including the key element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those within the art that：It still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (6)
1. a kind of micro-capsule loads plant source fish insect killing agent, it is characterised in that including active component and micro-capsule, the active component
Gained is extracted by the bulk drug containing following weight percentage component：Onion 28-30%, oleander 20-23%, gleditsia sinensis 10-
12%th, Chinese cassia tree 8-10%, honeysuckle 2-2.5%, short-tube lycoris 1-2%, Radix Astragali 1-2%, cordate houttuynia 0.5-1%, remaining is rice husk；Institute
Micro-capsule is stated to be prepared by beta-schardinger dextrin and succinic anhydride.
2. micro-capsule as claimed in claim 1 loads plant source fish insect killing agent, it is characterised in that the bulk drug contains following
The component of weight percentage：Onion 28-29%, oleander 21-22%, gleditsia sinensis 11-12%, Chinese cassia tree 9-10%, honeysuckle
2.2-2.5%, short-tube lycoris 1.5-2%, Radix Astragali 1-1.5%, cordate houttuynia 0.8-1%, remaining is rice husk.
3. micro-capsule as claimed in claim 1 or 2 loads plant source fish insect killing agent, it is characterised in that the oleander is selected from
Oleander fresh leaf and stem skin.
4. micro-capsule as claimed in claim 1 loads plant source fish insect killing agent, it is characterised in that preparation method includes following step
Suddenly：
(1) extraction of active component：Bulk drug is weighed according to weight percentage ratio, is crushed, by oleander, gleditsia sinensis and short-tube lycoris
Chloroform is immersed in, 55-60 DEG C of ultrasonic extraction 0.5h, is extracted 3 times, merges extract solution, be concentrated under reduced pressure to obtain medicinal extract 1, by the Radix Astragali and fish
Raw meat grass is immersed in 80% ethanol solution, and reserved filtrate is filtered after microwave abstracting 0.5h, and filter residue adds distilled water after being concentrated under reduced pressure,
Filtered after microwave abstracting 0.5h, after merging concentration filtrate, add absolute ethyl alcohol to adjust alcoholic degree to 80%, stand 8-12h, ultracentrifugation
Sediment is obtained, remaining bulk drug is immersed in 75% ethanol solution, refluxing extraction 1-2h, extracted 3 times, merges extract solution,
Be concentrated under reduced pressure to obtain medicinal extract 2；
(2) preparation of micro-capsule：Beta-schardinger dextrin is dissolved in 60 DEG C of hot water, succinic anhydride is added, 90 DEG C of reaction 0.5h of microwave, adds weak
Alkaline solution, chloroform extraction, concentrates organic phase, and ether precipitating produces beta-schardinger dextrin-succinate micro-capsule；
(3) preparation of insecticide：The medicinal extract 1, sediment and medicinal extract 2 of step (1) are dissolved in chloroform, add β-ring of step (2)
Dextrin-succinate micro-capsule, after reacting at room temperature 10-12h, concentration, ether precipitating, filtration drying obtains insecticide.
5. micro-capsule as claimed in claim 4 loads plant source fish insect killing agent, it is characterised in that the weakly alkaline solution is selected from
Saturated sodium bicarbonate solution, saturated sodium carbonate solution or 0.2molL-1Sodium hydroxide solution, preferably saturated sodium bicarbonate
Solution.
6. micro-capsule as claimed in claim 4 loads plant source fish insect killing agent, it is characterised in that the power of the microwave abstracting
It is 350W.
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| WO2009074879A2 (en) * | 2007-10-01 | 2009-06-18 | Nutrisystem Iphc, Inc. | Fiber and fatty acid composition and method of making same |
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| CN104872215A (en) * | 2015-05-27 | 2015-09-02 | 闭炳春 | Micro-encapsulated plant essential oil mosquito repellent containing cinnamon oil |
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