CN107373186A - A kind of bait of artemia culture and the method using biological flocculation artemia culture - Google Patents

A kind of bait of artemia culture and the method using biological flocculation artemia culture Download PDF

Info

Publication number
CN107373186A
CN107373186A CN201710515973.2A CN201710515973A CN107373186A CN 107373186 A CN107373186 A CN 107373186A CN 201710515973 A CN201710515973 A CN 201710515973A CN 107373186 A CN107373186 A CN 107373186A
Authority
CN
China
Prior art keywords
culture
biological flocculation
artemia
bait
water body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710515973.2A
Other languages
Chinese (zh)
Inventor
刘彦峰
何家瑞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Ocean Pal Carol Biotech Co Ltd
Original Assignee
Tianjin Ocean Pal Carol Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Ocean Pal Carol Biotech Co Ltd filed Critical Tianjin Ocean Pal Carol Biotech Co Ltd
Priority to CN201710515973.2A priority Critical patent/CN107373186A/en
Publication of CN107373186A publication Critical patent/CN107373186A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/042Introducing gases into the water, e.g. aerators, air pumps
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/10Inorganic compounds
    • C02F2101/16Nitrogen compounds, e.g. ammonia
    • C02F2101/166Nitrites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Water Supply & Treatment (AREA)
  • Environmental & Geological Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Hydrology & Water Resources (AREA)
  • Birds (AREA)
  • Insects & Arthropods (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Fodder In General (AREA)

Abstract

Method the invention provides a kind of bait of artemia culture and using biological flocculation artemia culture;The bait, including biological flocculation and eel powder, both mass ratioes are 4:1~8:1.Bait of the present invention, reduce the poisonous and harmful substances such as ammonia nitrogen in water body, nitrite nitrogen.Cultural method of the present invention, biological flocculation technology are decomposed and make use of the nutriment as probiotics such as portion of stool, improve energy utilization rate, reduce the residue of excrement, and important function is served to protection water quality.

Description

A kind of bait of artemia culture and the method using biological flocculation artemia culture
Technical field
The invention belongs to Technology of Artemia Culture field, bait and utilization biology wadding more particularly, to a kind of artemia culture The method of group's artemia culture.
Background technology
Artemia be it is a kind of be easy to culture, growth cycle is short, temperature and salinity adaptation scope are wide, nutritious one kind is without first Class lower animal, therefore it is a kind of biological living bait well.It is in recent years the cultivation of the aquatic livestocks such as China coast fish, shrimp Dealer is widely used.Although China is the abundant country of an artemia resource, but for the current market demand, artemia one All in a kind of state that supply falls short of demand since straight;Therefore, the highdensity quick cultivation of artemia how is improved, turns into and urgently solves Technical problem certainly.
The content of the invention
In view of this, the present invention is directed to propose a kind of bait of artemia culture and the side using biological flocculation artemia culture Method, with overcome the deficiencies in the prior art, realize artemia batch production high-density breeding.
To reach above-mentioned purpose, the technical proposal of the invention is realized in this way:
A kind of bait of artemia culture, including biological flocculation;Preferably, in addition to eel powder, both mass ratioes are 4:1 ~8:1.
Preferably, the mass ratio of biological flocculation and eel powder is 5:1~7:1;Preferably, 6:1.
Preferably, by biological flocculation and eel powder at 25~35 DEG C amphimicrobian ferment 12~24h;And biological flocculation And the particle diameter of eel powder be 300 mesh once, particle is the smaller the better.
Present invention simultaneously provides a kind of method using biological flocculation artemia culture, artemia culture as described above is used Bait, Artemia nauplii is accessed into breeding water body according to 1~4/mL density, second day starts, and bait is pressed According in daily 1~1.5mL/10L amount input breeding water body.
Preferably, breeding water body is the breeding water body after culture biological flocculation, and carries out filtering Air Exposure.
Preferably, at pseudimago phase to the adult stage, 4~6ppm Vc or B B-complex are added daily.
Preferably, in breeding process, the dissolved oxygen concentration in breeding water body is 2-10mg/L;It is 20~32 to cultivate temperature ℃;The pH of breeding water body is 7.8~8.5.
Preferably, the biological flocculation is cultivated as follows, to culture floc sedimentation water body in add probiotics, Carbon source, nitrogen source, using brown sugar as carbon source, using ammonium chloride as nitrogen source, carbon-nitrogen ratio is more than or equal to 13, and temperature is 28-32 DEG C, and dissolved oxygen 2~ 10ppm, lead to pure oxygen aeration culture 4-7 days, you can obtain bulky grain floc sedimentation, by filter, centrifuge, refrigerate, blend artemia is supported Little particle floc sedimentation needed for growing, refrigeration are stand-by;Preferably, probiotics is photosynthetic bacteria or lactic acid bacteria, and dissolved oxygen is 4~10ppm.
Preferably, dissolved oxygen is kept by the way of small tolerance intensity aeration oxygen replenishing.
The present invention also provides the application using the method for biological flocculation artemia culture in biological cultivation as described above.
Relative to prior art, the bait of artemia culture of the present invention and the side using biological flocculation artemia culture Method, have the advantage that:
(1) bait of the present invention, the poisonous and harmful substances such as ammonia nitrogen in water body, nitrite nitrogen are reduced.And biology wadding Group itself can grow required bait as artemia.
(2) cultural method of the present invention, biological flocculation technology are decomposed and make use of portion of stool etc. as probiotics Nutriment, energy utilization rate is improved, reduce the residue of excrement, important function is served to protection water quality.
(3) biology wadding team sheet provides nutrition as the growth of artemia individual, effectively reduces production cost, biology wadding Group can individually cultivate, and can also directly use caused biological flocculation in culturing pool, realize the recycling of resource.
(4) fermentation of animal/vegetable protein is that the material for making script artemia non-digestible becomes its absorption easy to digest Material, improve bait utilization.
(5) the crude fibre materials such as the powder of straw that is used in biological flocculation culture, alfalfa meal provide the same of implantation for probiotics When can stimulate the peristalsis frequency of artemia enteron aisle, add the absorptivity of bait.
(6) bait rationally after compounding can provide the more comprehensive nutrition needed for artemia individual growth.
(7) mode of small tolerance intensity aeration oxygen replenishing reduces outside machinery while ensure that water body dissolved oxygen abundance Power improves its survival rate to the individual injury of of artemia itself.
(8) factor such as accurate control dissolved oxygen, temperature, pH, throw-in density, to realizing that batch production high-density breeding serves Vital effect.
Embodiment
In addition to being defined, technical term used has universal with those skilled in the art of the invention in following examples The identical meanings of understanding.Test reagent used, is routine biochemistry reagent unless otherwise specified in following examples;It is described Experimental method, it is conventional method unless otherwise specified.
The present invention is described in detail with reference to embodiment.
Embodiment one
The acquisition of biological flocculation
Added into the water body of culture floc sedimentation photosynthetic bacteria, bacillus, the probiotics such as lactic acid bacteria, carbon source, nitrogen source and Alfalfa meal or powder of straw through ultra micro Freezing smashing, using brown sugar as carbon source, using ammonium chloride as nitrogen source, carbon-nitrogen ratio is more than or equal to 13, temperature is 28-32 DEG C, and dissolved oxygen is more than 4ppm, leads to pure oxygen aeration culture 4-7 days, you can acquisition bulky grain floc sedimentation, after by Little particle floc sedimentation needed for artemia culture is filtered, centrifuges, refrigerating, blending and to obtain, refrigeration is stand-by.
The configuration of bait
The biological flocculation of above-mentioned refrigeration is taken out, then with 6:1 ratio adds eel powder into above-mentioned biological flocculation, Amphimicrobian fermentation more than 24h under the conditions of 30 DEG C, it is standby to treat that fermentation is completed.
A kind of method using biological flocculation artemia culture, the cultivation water filtration of above-mentioned culture biological flocculation is aerated first (mode of small tolerance intensity aeration oxygen replenishing) is stand-by, secondly will hatch 24h Artemia nauplii by 2/ml's Density is accessed in above-mentioned breeding water body, starts within second day above-mentioned processed good bait putting into cultivation by daily 1ml/10L amounts Normally cultivated in water body, add 5ppm Vc or compound dimensions daily when artemia individual growth is to pseudimago phase to the adult stage The aqueous solution of raw element, just can make artemia individual grow to adult stage by the cultivation of 8 days or so.In whole breeding process also It should be noted some following problems:
1) in real time regulation aeration quantity size with ensure breeding water body dissolved oxygen abundance.Breeding water body dissolved oxygen is more than or equal to 4ppm。
2) control biological flocculation granular size, with ensure artemia can normally ingest these wadding team.
3) pH for controlling breeding water body is neutral meta-alkali, and most suitable is 7.8-8.5.
5) cultivation temperature cannot be below 20 DEG C or higher than 32 DEG C, be optimal using room temperature.
6) fermentation process of bait is complete.
Artemia culture according to the method described above, survival rate can reach 90%, obtain adult 21.37g/10L.
The preferred embodiment of the invention is the foregoing is only, is not intended to limit the invention creation, it is all at this Within the spirit and principle of innovation and creation, any modification, equivalent substitution and improvements made etc., the invention should be included in Protection domain within.

Claims (10)

  1. A kind of 1. bait of artemia culture, it is characterised in that:Including biological flocculation;Preferably, in addition to eel powder, both matter Amount is than being 4:1~8:1.
  2. 2. the bait of artemia culture according to claim 1, it is characterised in that:The mass ratio of biological flocculation and eel powder is 5:1~7:1;Preferably, 6:1.
  3. 3. the bait of artemia culture according to claim 1, it is characterised in that:By biological flocculation and eel powder 25~ 12~24h of amphimicrobian fermentation at 35 DEG C;And the particle diameter of biological flocculation and eel powder is below 300 mesh.
  4. A kind of 4. method using biological flocculation artemia culture, it is characterised in that:Using as described in any one of claim 1 or 2 Artemia culture bait, Artemia nauplii is accessed into breeding water body according to 1~4/mL density, is opened within second day Begin, by bait according in daily 1~1.5mL/10L amount input breeding water body.
  5. 5. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:Breeding water body is culture Breeding water body after biological flocculation, and carry out filtering Air Exposure.
  6. 6. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:The pseudimago phase into During the worm phase, 4~6ppm Vc or B B-complex are added daily.
  7. 7. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:In breeding process, Dissolved oxygen concentration in breeding water body is 2-10mg/L;It is 20~32 DEG C to cultivate temperature;The pH of breeding water body is 7.8~8.5.
  8. 8. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:The biological flocculation is Cultivate as follows, probiotics, carbon source, nitrogen source are added into the water body of culture floc sedimentation, using brown sugar as carbon source, with chlorination Ammonium is nitrogen source, and carbon-nitrogen ratio is more than or equal to 13, and temperature is 28-32 DEG C, 2~10ppm of dissolved oxygen, leads to pure oxygen aeration culture 4-7 days, you can Bulky grain floc sedimentation is obtained, by filtering, centrifuging, refrigerating, blending to obtain little particle floc sedimentation needed for artemia culture, refrigeration is stand-by;It is preferred that , probiotics be photosynthetic bacteria or lactic acid bacteria (I meant addition probiotics, such as photosynthetic bacteria originally, lactic acid bacteria, if Photosynthetic bacteria, bacillus, lactic acid bacteria are still write if writing preferred bacterium, is so write), dissolved oxygen is 4~10ppm.
  9. 9. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:Dissolved oxygen is using stingy The mode for measuring intensive aeration oxygen replenishing is kept.
  10. 10. the answering in biological cultivation of the method using biological flocculation artemia culture according to any one of claim 3~9 With.
CN201710515973.2A 2017-06-29 2017-06-29 A kind of bait of artemia culture and the method using biological flocculation artemia culture Pending CN107373186A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710515973.2A CN107373186A (en) 2017-06-29 2017-06-29 A kind of bait of artemia culture and the method using biological flocculation artemia culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710515973.2A CN107373186A (en) 2017-06-29 2017-06-29 A kind of bait of artemia culture and the method using biological flocculation artemia culture

Publications (1)

Publication Number Publication Date
CN107373186A true CN107373186A (en) 2017-11-24

Family

ID=60334439

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710515973.2A Pending CN107373186A (en) 2017-06-29 2017-06-29 A kind of bait of artemia culture and the method using biological flocculation artemia culture

Country Status (1)

Country Link
CN (1) CN107373186A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109601447A (en) * 2018-11-27 2019-04-12 中国水产科学研究院渔业机械仪器研究所 A kind of System and method for for realizing biological flocculation resource utilization
CN110856526A (en) * 2018-08-22 2020-03-03 南风化工集团股份有限公司 Brine shrimp feed for salt pan and preparation method thereof
CN115072883A (en) * 2022-06-17 2022-09-20 上海海洋大学 Biological floc particles and activation method and application thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103598124A (en) * 2013-11-15 2014-02-26 中国水产科学研究院珠江水产研究所 Method for ecologically preventing and treating epizootic ulcerative syndromes of fishes
CN105029111A (en) * 2015-08-28 2015-11-11 天津海友佳音生物科技股份有限公司 Bait for culturing large brine shrimps and culturing method thereof
CN105145431A (en) * 2015-08-21 2015-12-16 大连宝发海珍品有限公司 Method for cultivating and reinforcing fries of cynoglossus semilaevis gunther using biological floccules
CN105707016A (en) * 2016-03-08 2016-06-29 天津科技大学 Method for artemia culture in high salinity environment
CN106165790A (en) * 2016-08-31 2016-11-30 天津海友佳音生物科技股份有限公司 A kind of bait for artemia culture and artemia culture method
CN106719193A (en) * 2016-11-23 2017-05-31 福建省水产研究所 A kind of method of utilization biological flocculation technical optimization Environment of Litopenaeus vannamei Low seed rearing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103598124A (en) * 2013-11-15 2014-02-26 中国水产科学研究院珠江水产研究所 Method for ecologically preventing and treating epizootic ulcerative syndromes of fishes
CN105145431A (en) * 2015-08-21 2015-12-16 大连宝发海珍品有限公司 Method for cultivating and reinforcing fries of cynoglossus semilaevis gunther using biological floccules
CN105029111A (en) * 2015-08-28 2015-11-11 天津海友佳音生物科技股份有限公司 Bait for culturing large brine shrimps and culturing method thereof
CN105707016A (en) * 2016-03-08 2016-06-29 天津科技大学 Method for artemia culture in high salinity environment
CN106165790A (en) * 2016-08-31 2016-11-30 天津海友佳音生物科技股份有限公司 A kind of bait for artemia culture and artemia culture method
CN106719193A (en) * 2016-11-23 2017-05-31 福建省水产研究所 A kind of method of utilization biological flocculation technical optimization Environment of Litopenaeus vannamei Low seed rearing

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110856526A (en) * 2018-08-22 2020-03-03 南风化工集团股份有限公司 Brine shrimp feed for salt pan and preparation method thereof
CN109601447A (en) * 2018-11-27 2019-04-12 中国水产科学研究院渔业机械仪器研究所 A kind of System and method for for realizing biological flocculation resource utilization
CN115072883A (en) * 2022-06-17 2022-09-20 上海海洋大学 Biological floc particles and activation method and application thereof
CN115072883B (en) * 2022-06-17 2023-11-14 上海海洋大学 Biological floc particles and activation method and application thereof

Similar Documents

Publication Publication Date Title
CN103468613B (en) Bacillus megatherium, method for preparing microbial inoculum through solid fermentation of bacillus megatherium and application of microbial inoculum
CN107176691A (en) It is a kind of for spacetabs type microorganism improver of water quality of aquaculture and preparation method thereof
Banerjee et al. Phototrophic bacteria as fish feed supplement
CN103392642A (en) Method for breeding mandarin fish by utilizing microorganic feed
CN110731417B (en) Microbial fermentation feed for crayfish and preparation method thereof
CN1145734A (en) Quick-fermented feed, its preparation and uses
CN103242064B (en) Biological fish manure for aquaculture
CN107118983A (en) A kind of compound bacterial enzyme preparation for improving water quality and its preparation method and application
CN103689284B (en) A kind of bighead organic debris cenobium bait and preparation method thereof
CN107373186A (en) A kind of bait of artemia culture and the method using biological flocculation artemia culture
CN105754903A (en) Method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale
CN105052799A (en) Method for pond culture of cynoglossus semilaevis gunther by means of biofloc technique
CN106508821A (en) Method for breeding earthworms through combination of wastewater from cassava starch factories with municipal refuse
CN104982731B (en) A kind of method of waste water microalgae processing feed
CN103211088A (en) Preparation method of sea cucumber bait
CN103392930B (en) Method for cultivating Pseudobagrus fulvidraco in rice field
Jain et al. Low cost medium formulation using cow dung ash for the cultivation of Cyanobacterium: Spirulina (Arthrospira) platensis.
CN107637553A (en) Macrobrachium rosenbergii feeding method
CN109020667A (en) A kind of method and rich water material using black soldier flies cultivation excrement slag production rich water material
Ekpo et al. Digestibility of a commercial fish feed, wet algae, and dried algae by Tilapia nilotica and silver carp
CN106165790A (en) A kind of bait for artemia culture and artemia culture method
CN103396210B (en) Stichopus japonicus fertilizer, and preparation and application methods thereof
CN110800888A (en) Composition for culturing plankton, preparation method and application thereof
Vahdat et al. Long-term effects of vermicompost manure leachate (powder) inclusions on growth and survival, biochemical composition, total carotenoids, and broodstock reproductive performance of Artemia franciscana (Kellogg, 1906)
CN104293798A (en) Microbial preparation for reducing production of bottom sludge cake and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20171124