CN107373186A - A kind of bait of artemia culture and the method using biological flocculation artemia culture - Google Patents
A kind of bait of artemia culture and the method using biological flocculation artemia culture Download PDFInfo
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- CN107373186A CN107373186A CN201710515973.2A CN201710515973A CN107373186A CN 107373186 A CN107373186 A CN 107373186A CN 201710515973 A CN201710515973 A CN 201710515973A CN 107373186 A CN107373186 A CN 107373186A
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- 241001247197 Cephalocarida Species 0.000 title claims abstract description 43
- 238000005189 flocculation Methods 0.000 title claims abstract description 37
- 230000016615 flocculation Effects 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000843 powder Substances 0.000 claims abstract description 12
- 239000006041 probiotic Substances 0.000 claims abstract description 9
- 235000018291 probiotics Nutrition 0.000 claims abstract description 9
- 238000009395 breeding Methods 0.000 claims description 21
- 230000001488 breeding effect Effects 0.000 claims description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 17
- 229910052760 oxygen Inorganic materials 0.000 claims description 17
- 239000001301 oxygen Substances 0.000 claims description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 11
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- 238000004062 sedimentation Methods 0.000 claims description 9
- 238000005273 aeration Methods 0.000 claims description 8
- 241000238582 Artemia Species 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- 239000004310 lactic acid Substances 0.000 claims description 5
- 230000000243 photosynthetic effect Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 238000005057 refrigeration Methods 0.000 claims description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 3
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 abstract description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 2
- 231100000614 poison Toxicity 0.000 abstract description 2
- 230000007096 poisonous effect Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 241000219823 Medicago Species 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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Abstract
Method the invention provides a kind of bait of artemia culture and using biological flocculation artemia culture;The bait, including biological flocculation and eel powder, both mass ratioes are 4:1~8:1.Bait of the present invention, reduce the poisonous and harmful substances such as ammonia nitrogen in water body, nitrite nitrogen.Cultural method of the present invention, biological flocculation technology are decomposed and make use of the nutriment as probiotics such as portion of stool, improve energy utilization rate, reduce the residue of excrement, and important function is served to protection water quality.
Description
Technical field
The invention belongs to Technology of Artemia Culture field, bait and utilization biology wadding more particularly, to a kind of artemia culture
The method of group's artemia culture.
Background technology
Artemia be it is a kind of be easy to culture, growth cycle is short, temperature and salinity adaptation scope are wide, nutritious one kind is without first
Class lower animal, therefore it is a kind of biological living bait well.It is in recent years the cultivation of the aquatic livestocks such as China coast fish, shrimp
Dealer is widely used.Although China is the abundant country of an artemia resource, but for the current market demand, artemia one
All in a kind of state that supply falls short of demand since straight;Therefore, the highdensity quick cultivation of artemia how is improved, turns into and urgently solves
Technical problem certainly.
The content of the invention
In view of this, the present invention is directed to propose a kind of bait of artemia culture and the side using biological flocculation artemia culture
Method, with overcome the deficiencies in the prior art, realize artemia batch production high-density breeding.
To reach above-mentioned purpose, the technical proposal of the invention is realized in this way:
A kind of bait of artemia culture, including biological flocculation;Preferably, in addition to eel powder, both mass ratioes are 4:1
~8:1.
Preferably, the mass ratio of biological flocculation and eel powder is 5:1~7:1;Preferably, 6:1.
Preferably, by biological flocculation and eel powder at 25~35 DEG C amphimicrobian ferment 12~24h;And biological flocculation
And the particle diameter of eel powder be 300 mesh once, particle is the smaller the better.
Present invention simultaneously provides a kind of method using biological flocculation artemia culture, artemia culture as described above is used
Bait, Artemia nauplii is accessed into breeding water body according to 1~4/mL density, second day starts, and bait is pressed
According in daily 1~1.5mL/10L amount input breeding water body.
Preferably, breeding water body is the breeding water body after culture biological flocculation, and carries out filtering Air Exposure.
Preferably, at pseudimago phase to the adult stage, 4~6ppm Vc or B B-complex are added daily.
Preferably, in breeding process, the dissolved oxygen concentration in breeding water body is 2-10mg/L;It is 20~32 to cultivate temperature
℃;The pH of breeding water body is 7.8~8.5.
Preferably, the biological flocculation is cultivated as follows, to culture floc sedimentation water body in add probiotics,
Carbon source, nitrogen source, using brown sugar as carbon source, using ammonium chloride as nitrogen source, carbon-nitrogen ratio is more than or equal to 13, and temperature is 28-32 DEG C, and dissolved oxygen 2~
10ppm, lead to pure oxygen aeration culture 4-7 days, you can obtain bulky grain floc sedimentation, by filter, centrifuge, refrigerate, blend artemia is supported
Little particle floc sedimentation needed for growing, refrigeration are stand-by;Preferably, probiotics is photosynthetic bacteria or lactic acid bacteria, and dissolved oxygen is 4~10ppm.
Preferably, dissolved oxygen is kept by the way of small tolerance intensity aeration oxygen replenishing.
The present invention also provides the application using the method for biological flocculation artemia culture in biological cultivation as described above.
Relative to prior art, the bait of artemia culture of the present invention and the side using biological flocculation artemia culture
Method, have the advantage that:
(1) bait of the present invention, the poisonous and harmful substances such as ammonia nitrogen in water body, nitrite nitrogen are reduced.And biology wadding
Group itself can grow required bait as artemia.
(2) cultural method of the present invention, biological flocculation technology are decomposed and make use of portion of stool etc. as probiotics
Nutriment, energy utilization rate is improved, reduce the residue of excrement, important function is served to protection water quality.
(3) biology wadding team sheet provides nutrition as the growth of artemia individual, effectively reduces production cost, biology wadding
Group can individually cultivate, and can also directly use caused biological flocculation in culturing pool, realize the recycling of resource.
(4) fermentation of animal/vegetable protein is that the material for making script artemia non-digestible becomes its absorption easy to digest
Material, improve bait utilization.
(5) the crude fibre materials such as the powder of straw that is used in biological flocculation culture, alfalfa meal provide the same of implantation for probiotics
When can stimulate the peristalsis frequency of artemia enteron aisle, add the absorptivity of bait.
(6) bait rationally after compounding can provide the more comprehensive nutrition needed for artemia individual growth.
(7) mode of small tolerance intensity aeration oxygen replenishing reduces outside machinery while ensure that water body dissolved oxygen abundance
Power improves its survival rate to the individual injury of of artemia itself.
(8) factor such as accurate control dissolved oxygen, temperature, pH, throw-in density, to realizing that batch production high-density breeding serves
Vital effect.
Embodiment
In addition to being defined, technical term used has universal with those skilled in the art of the invention in following examples
The identical meanings of understanding.Test reagent used, is routine biochemistry reagent unless otherwise specified in following examples;It is described
Experimental method, it is conventional method unless otherwise specified.
The present invention is described in detail with reference to embodiment.
Embodiment one
The acquisition of biological flocculation
Added into the water body of culture floc sedimentation photosynthetic bacteria, bacillus, the probiotics such as lactic acid bacteria, carbon source, nitrogen source and
Alfalfa meal or powder of straw through ultra micro Freezing smashing, using brown sugar as carbon source, using ammonium chloride as nitrogen source, carbon-nitrogen ratio is more than or equal to
13, temperature is 28-32 DEG C, and dissolved oxygen is more than 4ppm, leads to pure oxygen aeration culture 4-7 days, you can acquisition bulky grain floc sedimentation, after by
Little particle floc sedimentation needed for artemia culture is filtered, centrifuges, refrigerating, blending and to obtain, refrigeration is stand-by.
The configuration of bait
The biological flocculation of above-mentioned refrigeration is taken out, then with 6:1 ratio adds eel powder into above-mentioned biological flocculation,
Amphimicrobian fermentation more than 24h under the conditions of 30 DEG C, it is standby to treat that fermentation is completed.
A kind of method using biological flocculation artemia culture, the cultivation water filtration of above-mentioned culture biological flocculation is aerated first
(mode of small tolerance intensity aeration oxygen replenishing) is stand-by, secondly will hatch 24h Artemia nauplii by 2/ml's
Density is accessed in above-mentioned breeding water body, starts within second day above-mentioned processed good bait putting into cultivation by daily 1ml/10L amounts
Normally cultivated in water body, add 5ppm Vc or compound dimensions daily when artemia individual growth is to pseudimago phase to the adult stage
The aqueous solution of raw element, just can make artemia individual grow to adult stage by the cultivation of 8 days or so.In whole breeding process also
It should be noted some following problems:
1) in real time regulation aeration quantity size with ensure breeding water body dissolved oxygen abundance.Breeding water body dissolved oxygen is more than or equal to
4ppm。
2) control biological flocculation granular size, with ensure artemia can normally ingest these wadding team.
3) pH for controlling breeding water body is neutral meta-alkali, and most suitable is 7.8-8.5.
5) cultivation temperature cannot be below 20 DEG C or higher than 32 DEG C, be optimal using room temperature.
6) fermentation process of bait is complete.
Artemia culture according to the method described above, survival rate can reach 90%, obtain adult 21.37g/10L.
The preferred embodiment of the invention is the foregoing is only, is not intended to limit the invention creation, it is all at this
Within the spirit and principle of innovation and creation, any modification, equivalent substitution and improvements made etc., the invention should be included in
Protection domain within.
Claims (10)
- A kind of 1. bait of artemia culture, it is characterised in that:Including biological flocculation;Preferably, in addition to eel powder, both matter Amount is than being 4:1~8:1.
- 2. the bait of artemia culture according to claim 1, it is characterised in that:The mass ratio of biological flocculation and eel powder is 5:1~7:1;Preferably, 6:1.
- 3. the bait of artemia culture according to claim 1, it is characterised in that:By biological flocculation and eel powder 25~ 12~24h of amphimicrobian fermentation at 35 DEG C;And the particle diameter of biological flocculation and eel powder is below 300 mesh.
- A kind of 4. method using biological flocculation artemia culture, it is characterised in that:Using as described in any one of claim 1 or 2 Artemia culture bait, Artemia nauplii is accessed into breeding water body according to 1~4/mL density, is opened within second day Begin, by bait according in daily 1~1.5mL/10L amount input breeding water body.
- 5. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:Breeding water body is culture Breeding water body after biological flocculation, and carry out filtering Air Exposure.
- 6. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:The pseudimago phase into During the worm phase, 4~6ppm Vc or B B-complex are added daily.
- 7. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:In breeding process, Dissolved oxygen concentration in breeding water body is 2-10mg/L;It is 20~32 DEG C to cultivate temperature;The pH of breeding water body is 7.8~8.5.
- 8. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:The biological flocculation is Cultivate as follows, probiotics, carbon source, nitrogen source are added into the water body of culture floc sedimentation, using brown sugar as carbon source, with chlorination Ammonium is nitrogen source, and carbon-nitrogen ratio is more than or equal to 13, and temperature is 28-32 DEG C, 2~10ppm of dissolved oxygen, leads to pure oxygen aeration culture 4-7 days, you can Bulky grain floc sedimentation is obtained, by filtering, centrifuging, refrigerating, blending to obtain little particle floc sedimentation needed for artemia culture, refrigeration is stand-by;It is preferred that , probiotics be photosynthetic bacteria or lactic acid bacteria (I meant addition probiotics, such as photosynthetic bacteria originally, lactic acid bacteria, if Photosynthetic bacteria, bacillus, lactic acid bacteria are still write if writing preferred bacterium, is so write), dissolved oxygen is 4~10ppm.
- 9. the method according to claim 3 using biological flocculation artemia culture, it is characterised in that:Dissolved oxygen is using stingy The mode for measuring intensive aeration oxygen replenishing is kept.
- 10. the answering in biological cultivation of the method using biological flocculation artemia culture according to any one of claim 3~9 With.
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CN110856526A (en) * | 2018-08-22 | 2020-03-03 | 南风化工集团股份有限公司 | Brine shrimp feed for salt pan and preparation method thereof |
CN115072883A (en) * | 2022-06-17 | 2022-09-20 | 上海海洋大学 | Biological floc particles and activation method and application thereof |
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Cited By (4)
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CN110856526A (en) * | 2018-08-22 | 2020-03-03 | 南风化工集团股份有限公司 | Brine shrimp feed for salt pan and preparation method thereof |
CN109601447A (en) * | 2018-11-27 | 2019-04-12 | 中国水产科学研究院渔业机械仪器研究所 | A kind of System and method for for realizing biological flocculation resource utilization |
CN115072883A (en) * | 2022-06-17 | 2022-09-20 | 上海海洋大学 | Biological floc particles and activation method and application thereof |
CN115072883B (en) * | 2022-06-17 | 2023-11-14 | 上海海洋大学 | Biological floc particles and activation method and application thereof |
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