CN107372638A - A kind of natural antibacterial extract of suitable Medical rag and its preparation method and application - Google Patents
A kind of natural antibacterial extract of suitable Medical rag and its preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47L—DOMESTIC WASHING OR CLEANING; SUCTION CLEANERS IN GENERAL
- A47L13/00—Implements for cleaning floors, carpets, furniture, walls, or wall coverings
- A47L13/10—Scrubbing; Scouring; Cleaning; Polishing
- A47L13/16—Cloths; Pads; Sponges
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- General Health & Medical Sciences (AREA)
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- Plant Pathology (AREA)
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- Agronomy & Crop Science (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of natural antibacterial extract of suitable Medical rag and its preparation method and application, the natural antibacterial extract be the vegetable material of following weight is pre-processed, low temperature distillation and ether extraction and recovery obtain：15 25 parts of honeysuckle, 15 25 parts of artemisia annua, 46 parts of peppermint, 46 parts of Chinese weeping cypress leaf.Confirmed through bacteriostatic experiment, more than 90.0%, effect protrudes natural antibacterial extract bacteriostasis rate of the invention.In addition, its raw material sources is extensive, extraction step is simple, low for equipment requirements, and solvent load is few, therefore cost is cheap, is very suitable as daily natural antibacterial agent, particularly suitable for applying or infiltrating on Medical rag, the bactericidal cleaning for hospital.
Description
Technical field
The present invention relates to the extraction of natural antimicrobial substance, specifically, is related to a kind of natural antibacterial of suitable Medical rag
Extract and its preparation method and application.
Background technology
Rag is the cloth that implements are wiped in daily life, in the public affairs such as family, hotel, office space and hospital, cinema
Place, the use of rag are ubiquitous altogether.The main function of rag is exactly to remove dust, the spot on implements surface etc., therefore meeting
Be infected with the pathogenic microorganisms such as substantial amounts of bacterium, such as cannot thoroughly clean, the pathogenic microorganism in rag then can amount reproduction, make
Rag turns into the infection sources, and cross transmission is caused during reusing, is extremely unfavorable for health.Especially in hospital so
Place, inherently have a large amount of patients for carrying germs, and flow of the people is very big, the interface such as seat, escalator handrail all exists a large amount of
Pathogenic bacteria, especially it is noted that keeping the sanitation and hygiene of rag, prevent cross transmission, it is also noted that these interfaces are carried out regular
Disinfect, remove pathogeny in time, prevent from being propagated further.Therefore, if can be applied on rag or immerse antibacterial decoction
Deng can then play a part of carrying out disinfection to rag, moreover it is possible to the surface of wiping is carried out disinfection in use.
Excitant be present in the conventional use of disinfectant of Hospitals at Present, such as 84 thimerosals, respiratory tract, skin to people etc., and
Be not suitable for widely using in wiping process.Substantial amounts of natural antimicrobial substance in animals and plants be present, such as Tea Polyphenols, licorice polysaccharide
Deng.Often effective component species is more in animals and plants natural extract, different effective components can play collaboration etc. reciprocation, compared with
Satisfied antibacterial effect is achieved with low dose, especially animals and plants natural antimicrobial substance security is good, to human body and environment
It is minimum in the absence of harm or side effect, therefore safety and environmental protection.
Patent document CN101085795A, publication date 2007.12.12, disclose from Flos lonicerae or its Original plant
Honeysuckle or the method for belonging to prepared plant extracts in other plant together, and the Honegsukle flower P.E prepared by this method, and contain
There is the pharmaceutical composition of the extract, the active ingredient of the composition is secologanic acid and its derivative species chemical combination
Thing, this method include water or alcohol extracting, and extract refines through precipitation and chromatographic process, if necessary to also can use gel column chromatography to enter
One step purifies, and the content of secologanic acid and its derivative is more than 50% in obtained extract, and preferably more than 70%,
The Honegsukle flower P.E obtained with the preparation method of the invention has antipyretic, analgesia, anti-inflammatory, antibacterial and antiviral activity, can
For treating the diseases such as hyperpyrexia, pain and infectious diseases, especially viral pneumonia, virus flu and AIDS.Specially
Sharp document CN102727611A, publication date 2012.10.17, disclose a kind of external application Chinese medicine that effect is repaired with anti-bacterial, anti-itching
Composition, preparation and preparation method, the bulk drug that the composition is matched by following weight parts form：The weight of cloves 20~50
Part, the parts by weight of the red sage root 20~50, the parts by weight of folium artemisiae argyi 20~50, the parts by weight of hawthorn 10~20, the parts by weight of sea-buckthorn 10~20, tribulus terrestris
10~20 parts by weight, the parts by weight of sweet wormwood 10~20, the parts by weight of giant knotweed 10~20, preparation method comprise the following steps that：(1) according to institute
State weight and weigh each bulk drug；(2) wherein cloves, the red sage root, hawthorn and sea-buckthorn mixing, adding percent by volume is
80%-95% ethanol, solid-liquid ratio g/ml are 1: 20~1: 30；Extracting temperature is 40~70 DEG C, and extraction time is that 0.5~3h is obtained
Alcohol extract；Folium artemisiae argyi, tribulus terrestris, sweet wormwood and giant knotweed mixing, extracting in water, solid-liquid ratio 1: 20~1: 30；Extracting temperature be 50~
90 DEG C, extraction time is that 1~4h obtains Aqueous extracts；(3) alcohol extract and Aqueous extracts obtained step (2) filters respectively, and then two
Kind of extract solution is well mixed to be concentrated into 1~5g/ml and obtains concentrate；(4) concentrate mixes to obtain mixed liquor with glycerine, concentrate with it is sweet
The volume ratio of oil is 1: 1~2: 1；(5) mixed liquor is filtered, obtains activity extract.
However, it is still very necessary to develop more natural antibacterial extracts, especially yet there are no suitable for being smeared medical
Used on cloth, antibacterial effect protrudes, and is easy to the natural antibacterial extract prepared, cost is low.
The content of the invention
The purpose of the present invention is to be directed to deficiency of the prior art, there is provided a kind of natural antibacterial extraction of suitable Medical rag
Thing.
Another purpose of the present invention is to provide the preparation method of described natural antibacterial extract.
Another purpose of the present invention is to provide the purposes of described natural antibacterial extract.
Fourth object of the present invention is to provide a kind of Medical rag.
To realize above-mentioned first purpose, the present invention adopts the technical scheme that：
A kind of natural antibacterial extract of suitable Medical rag, the preparation method of described natural antibacterial extract include with
Lower step：
A) pretreatment of raw material：Following raw material is cleaned, dried, 8-10cm fritters are made：Honeysuckle, artemisia annua, peppermint, cypress
Leaf；
B) low temperature distillation：Raw material residue is weighed, adds deionized water immersion 3-5h, 60-80 DEG C of heating distillation extraction 1-3h；
C) reclaim：After distillation, with washed with ether condenser pipe, after abundant layering, ether layer is collected, is steamed using rotation
Instrument is sent out, is decompressed to 400-600 millimetress of mercury, heats the solvent in cucurbit with heating bath, heating-up temperature is 35-40 DEG C, simultaneously
Rotated, speed is 50~160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil.
Preferably, the weight of each raw material is in step a)：Honeysuckle 15-25 parts, artemisia annua 15-25 parts, peppermint
4-6 parts, Chinese weeping cypress leaf 4-6 parts.
It is highly preferred that the weight of each raw material is in step a)：20 parts of honeysuckle, 20 parts of artemisia annua, 5 parts of peppermint,
5 parts of Chinese weeping cypress leaf.
Preferably, the deionized water described in step b) and the solid-liquid ratio of raw material residue are 10-40g:250-300mL.
To realize above-mentioned second purpose, the present invention adopts the technical scheme that：
The preparation method of described natural antibacterial extract, comprises the following steps：
A) pretreatment of raw material：Following raw material is cleaned, dried, 8-10cm fritters are made：Honeysuckle, artemisia annua, peppermint, cypress
Leaf；
B) low temperature distillation：Raw material residue is weighed, adds deionized water immersion 3-5h, 60-80 DEG C of heating distillation extraction 1-3h；
C) reclaim：After distillation, with washed with ether condenser pipe, after abundant layering, ether layer is collected, is steamed using rotation
Instrument is sent out, is decompressed to 400-600 millimetress of mercury, heats the solvent in cucurbit with heating bath, heating-up temperature is 35-40 DEG C, simultaneously
Rotated, speed is 50~160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil.
Preferably, the weight of each raw material is in step a)：Honeysuckle 15-25 parts, artemisia annua 15-25 parts, peppermint
4-6 parts, Chinese weeping cypress leaf 4-6 parts.
It is highly preferred that the weight of each raw material is in step a)：20 parts of honeysuckle, 20 parts of artemisia annua, 5 parts of peppermint,
5 parts of Chinese weeping cypress leaf.
Preferably, the deionized water described in step b) and the solid-liquid ratio of raw material residue are 10-40g:250-300mL.
To realize above-mentioned 3rd purpose, the present invention adopts the technical scheme that：
As above application of any described natural antibacterial extract in antibacterials are prepared.
As a concrete example, described antibacterials are to suppress Escherichia coli, staphylococcus aureus or Candida albicans
The medicine of bacterium.
As a concrete example, described antibacterials are to act on the medicine of human body or act in environment with degerming
Medicine, the medicine for acting on human body include external used medicine and endo-medicine.
To realize above-mentioned 4th purpose, the present invention adopts the technical scheme that：
A kind of Medical rag, described Medical rag contain as above any described natural antibacterial extract.
As a concrete example, described Medical rag can be cotton gauze, linen, non-woven fabrics material, but not only
It is limited to this.
Herein, described honeysuckle also known as honeysuckle, scientific name：Lonicera japonica；Described artemisia annua, scientific name：
Artemisia annua Linn., it is composite family artemisia annual herb plant；Described peppermint scientific name Mentha
Haplocalyx Briq., described Chinese weeping cypress leaf scientific name Platycladus orientalis (L.) Francoptmxjjkmsc,
For a kind of aiphyllium of Cupressaceae Platycladus, it is used as medicine with its leaf and withe.
The invention has the advantages that：
1st, present invention extraction from honeysuckle, artemisia annua, peppermint, Chinese weeping cypress leaf obtains the material with antibacterial activity, antibacterial
Test result indicates that the bacteriostasis rate of the natural antibacterial extract, more than 90.0%, effect is very prominent；
2nd, the equal planting range of each raw material is big, and wide material sources, price is low, therefore the natural antibacterial extract cost of the present invention
It is low；
3rd, the preparation method of natural antibacterial extract of the present invention uses low temperature distillation, and step is simple, low for equipment requirements, makes
Solvent is few, and pollution is small；
4th, the present invention by the natural antibacterial extract of extraction with Medical rag, suitable for being used in hospital's occasion, not only
Sterilizing can be carried out to rag, also sterilizing can be carried out to the interface of wiping during rag use, effectively reduce doctor
The breeding and propagation of institute occasion germ, there is important practical significance.
Brief description of the drawings
Accompanying drawing 1 is the natural antibacterial extract gas chromatography-mass spectrography spectrogram of embodiment 2.
Embodiment
Embodiment provided by the invention is elaborated below in conjunction with the accompanying drawings.
The natural antibacterial extract (one) of the present invention of embodiment 1
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：25 parts of honeysuckle, 15 parts of artemisia annua, 6 parts of peppermint, 4 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 10g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 3h, 60 DEG C of heating distillation extraction 1h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 600 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 40 DEG C, is carried out simultaneously
Rotation, speed are 160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
The natural antibacterial extract (two) of the present invention of embodiment 2
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：20 parts of honeysuckle, 20 parts of artemisia annua, 5 parts of peppermint, 5 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 10g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 4h, 70 DEG C of heating distillation extraction 2h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 500 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 35 DEG C, is carried out simultaneously
Rotation, speed are 100 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
The natural antibacterial extract (three) of the present invention of embodiment 3
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：15 parts of honeysuckle, 25 parts of artemisia annua, 4 parts of peppermint, 6 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 10g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
250mL soaks 5h, 80 DEG C of heating distillation extraction 3h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 400 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 35 DEG C, is carried out simultaneously
Rotation, speed are 50 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
The natural antibacterial extract (four) of the present invention of embodiment 4
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：20 parts of honeysuckle, 20 parts of artemisia annua, 5 parts of peppermint, 5 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 40g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 3h, 60 DEG C of heating distillation extraction 1h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 600 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 40 DEG C, is carried out simultaneously
Rotation, speed are 160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
The natural antibacterial extract (five) of the present invention of embodiment 5
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：15 parts of honeysuckle, 25 parts of artemisia annua, 4 parts of peppermint, 6 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 40g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 3h, 60 DEG C of heating distillation extraction 2h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 600 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 40 DEG C, is carried out simultaneously
Rotation, speed are 160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
The natural antibacterial extract (six) of the present invention of embodiment 6
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：15 parts of honeysuckle, 25 parts of artemisia annua, 4 parts of peppermint, 6 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 40g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 4h, 70 DEG C of heating distillation extraction 3h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 500 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 35 DEG C, is carried out simultaneously
Rotation, speed are 100 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
Comparative example 1
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：25 parts of honeysuckle, 4 parts of artemisia annua, 6 parts of peppermint, 15 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 10g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 3h, 60 DEG C of heating distillation extraction 1h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 600 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 40 DEG C, is carried out simultaneously
Rotation, speed are 160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
Comparative example 2
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：20 parts of honeysuckle, 20 parts of wormwood, 5 parts of peppermint, 5 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 10g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 4h, 70 DEG C of heating distillation extraction 2h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 500 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 35 DEG C, is carried out simultaneously
Rotation, speed are 100 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
Comparative example 3
A) vegetable material pre-processes：By following weight fresh plant materials, it is washed with deionized water only, is placed in cool place
Place is dried, and 8-10cm fritters are cut into scissors：25 parts of honeysuckle, 15 parts of artemisia annua, 6 parts of peppermint, 4 parts of Chinese weeping cypress leaf；
B) low temperature distillation：The plant residue 10g shredded is weighed, is put in 500mL round-bottomed flasks, adds deionized water
300mL soaks 3h, 60 DEG C of heating distillation extraction 1h；
C) reclaim：After distillation, with a small amount of washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotation
Turn evaporimeter, be decompressed to 600 millimetress of mercury, heat the solvent in cucurbit with heating bath, heating-up temperature is 45 DEG C, is carried out simultaneously
Rotation, speed are 160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil, precise quantitative, 4 DEG C
Refrigerator saves backup.
The antibacterial effect checking of each natural antibacterial extract of embodiment 7
Use the antibacterial effect of Quine's method detection antibiotic fabric.
1 experiment material
Embodiment 1-6 and comparative example 1-3 natural antibacterial extract.
Bacterial strain：Escherichia coli ATCC8739, staphylococcus aureus ATCC25923, Candida albicans ATCC10231, purchase
In Shanghai Yan Sheng Industrial Co., Ltd.s.
2 experimental methods
2.1 prepare anti-biotic material
Example 1-6 and comparative example 1-3 natural antibacterial extract 1ml respectively, drops in rag (cotton after high-temperature sterilization
Gauze) on (size：2.5cm*2.5cm), blank rag (cotton gauze) and standard blank sample (filter paper) are taken in addition, and size is same
On.
2.2 inoculation
In blank culture dish, Antibacterial rag, blank rag, standard blank sample are completed, by standard bacterium solution (1*
105Cfu/ml) 100-200 μ l are dropped on rag or on blank sample；It is positioned in biochemical cultivation case and dries；By dry style
Take out, be positioned on agar medium, post, then be inverted semisolid culturemedium uniform fold style.
2.3 cultures count
Placed in incubator 48 hours, 37 DEG C or so errors are no more than 1 DEG C, count clump count, calculate bacteriostasis rate.It is above-mentioned
3 parallel laboratory tests are set up in experiment, are averaged, in order to avoid experimental error.
3 experimental results
Natural antibacterial extract prepared by embodiment 1-6 and comparative example 1-3 is shown in Table 1-3 to the antibacterial effect of each bacterial strain.From
As a result as can be seen that natural antibacterial extract prepared by embodiment 1-6 is easy to pathogenic bacteria large intestine bar contagious to common
Bacterium, staphylococcus aureus and Candida albicans have significant inhibitory action, and bacteriostasis rate is more than 90.0%.Comparative example 1-3
The natural antibacterial extract of preparation is significantly smaller than embodiment 1-6 (p to the antibacterial effect of each bacterial strain<0.05) plant material, is shown
The temperature setting that ether is removed in the selection of material and extraction step has certain shadow for the species and activity of antibacterial substance
Ring.
Antibacterial action of each natural antibacterial extract of table 1 to Escherichia coli
| Group | Standard blank bacterium colony | Blank rag bacterium colony | * antibacterial rag bacterium colony | Bacteriostasis rate (%) |
| Embodiment 1 | >200 | >200 | 12 | 94.0 |
| Embodiment 2 | >200 | >200 | 8 | 96.0 |
| Embodiment 3 | >200 | >200 | 20 | 90.0 |
| Embodiment 4 | >200 | >200 | 15 | 92.5 |
| Embodiment 5 | >200 | >200 | 12 | 94.0 |
| Embodiment 6 | >200 | >200 | 11 | 94.5 |
| Comparative example 1 | >200 | >200 | 77 | 61.5 |
| Comparative example 2 | >200 | >200 | 83 | 58.5 |
| Comparative example 3 | >200 | >200 | 65 | 67.5 |
Antibacterial action of each natural antibacterial extract of table 2 to staphylococcus aureus
| Group | Standard blank bacterium colony | Blank rag bacterium colony | * antibacterial rag bacterium colony | Bacteriostasis rate (%) |
| Embodiment 1 | >200 | >200 | 7 | 96.5 |
| Embodiment 2 | >200 | >200 | 8 | 96.0 |
| Embodiment 3 | >200 | >200 | 5 | 97.5 |
| Embodiment 4 | >200 | >200 | 9 | 95.5 |
| Embodiment 5 | >200 | >200 | 9 | 95.5 |
| Embodiment 6 | >200 | >200 | 10 | 95.0 |
| Comparative example 1 | >200 | >200 | 85 | 57.5 |
| Comparative example 2 | >200 | >200 | 76 | 62.0 |
| Comparative example 3 | >200 | >200 | 73 | 63.5 |
Antibacterial action of each natural antibacterial extract of table 3 to Candida albicans
Note：Above clump count is colony counts every square centimeter, when clump count is more than 200, during bacteriostasis rate calculates, and blank
Rag clump count is in terms of 200.
The chemical composition detection of the natural antibacterial extract of embodiment 8
Detection method：Agilent company of the U.S. 7890A-5975C gas chromatograph-mass spectrometer (GC-MS)s (GC-MS)；Chromatographic column：
DB-wax(30m×0.25mm×0.25μm)；Sample size：250ml；Injector temperature：260℃；Split ratio：Without shunting；Carrier gas：Helium
Gas (99.999%)；Flow：1mL/min；Column temperature：40 DEG C of holding 5min, rise to 250 DEG C with 5 DEG C/min, keep 5min；Interface
Temperature：260℃；Ion source temperature：230℃；Level Four bar temperature：150℃；Ionization mode：EI+, 70ev；Detector voltage：
2200V；Scan mode：Full scan；Mass range：20~400；NIST 2011 composes storehouse.
Testing result：Each main component and its relative amount and during appearance in natural antibacterial extract prepared by embodiment 2
Between be shown in Table 4.
Main component and its relative amount and appearance time in the natural antibacterial extract of table 4
| Main component | Relative amount (%) | Appearance time (min) |
| Hexamethylene monoterpene derivative (camphor) | 13.51 | 21 |
| Myrtenol | 5.98 | 27 |
| 5,5- dimethyl -1,3- hexadienes | 11.59 | 21 |
| Cyclopentene group | 4.61 | 25 |
| Bornylene | 3.55 | 23 |
| Eucalyptol | 5.52 | 12 |
| Heptadiene | 3.91 | 18 |
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
Member, on the premise of the inventive method is not departed from, can also make some improvement and supplement, and these are improved and supplement also should be regarded as
Protection scope of the present invention.
Claims (10)
- A kind of 1. natural antibacterial extract of suitable Medical rag, it is characterised in that the preparation of described natural antibacterial extract Method comprises the following steps：A) pretreatment of raw material：Following raw material is cleaned, dried, 8-10cm fritters are made：Honeysuckle, artemisia annua, peppermint, cypress Leaf；B) low temperature distillation：Raw material residue is weighed, adds deionized water immersion 3-5h, 60-80 DEG C of heating distillation extraction 1-3h；C) reclaim：After distillation, with washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotary evaporation Instrument, 400-600 millimetress of mercury are decompressed to, heat the solvent in cucurbit with heating bath, heating-up temperature is 35-40 DEG C, is entered simultaneously Row rotation, speed are 50~160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil.
- 2. natural antibacterial extract according to claim 1, it is characterised in that the weight of each raw material in step a) For：Honeysuckle 15-25 parts, artemisia annua 15-25 parts, peppermint 4-6 parts, Chinese weeping cypress leaf 4-6 parts.
- 3. natural antibacterial extract according to claim 2, it is characterised in that the weight of each raw material in step a) For：20 parts of honeysuckle, 20 parts of artemisia annua, 5 parts of peppermint, 5 parts of Chinese weeping cypress leaf.
- 4. natural antibacterial extract according to claim 1, it is characterised in that deionized water and original described in step b) The solid-liquid ratio for expecting residue is 10-40g:250-300mL.
- 5. the preparation method of the natural antibacterial extract described in claim 1, it is characterised in that comprise the following steps：A) pretreatment of raw material：Following raw material is cleaned, dried, 8-10cm fritters are made：Honeysuckle, artemisia annua, peppermint, cypress Leaf；B) low temperature distillation：Raw material residue is weighed, adds deionized water immersion 3-5h, 60-80 DEG C of heating distillation extraction 1-3h；C) reclaim：After distillation, with washed with ether condenser pipe, after abundant layering, ether layer is collected, using rotary evaporation Instrument, 400-600 millimetress of mercury are decompressed to, heat the solvent in cucurbit with heating bath, heating-up temperature is 35-40 DEG C, is entered simultaneously Row rotation, speed are 50~160 revs/min, and it is described natural antibacterial extract to volatilize ether and obtain yellow oil.
- 6. preparation method according to claim 5, it is characterised in that the weight of each raw material is in step a)：Gold Honeysuckle flower 15-25 parts, artemisia annua 15-25 parts, peppermint 4-6 parts, Chinese weeping cypress leaf 4-6 parts.
- 7. preparation method according to claim 6, it is characterised in that the weight of each raw material is in step a)：Gold 20 parts of honeysuckle flower, 20 parts of artemisia annua, 5 parts of peppermint, 5 parts of Chinese weeping cypress leaf.
- 8. preparation method according to claim 5, it is characterised in that deionized water and raw material residue described in step b) Solid-liquid ratio be 10-40g:250-300mL.
- 9. application of any described natural antibacterial extracts of claim 1-4 in antibacterials are prepared.
- 10. a kind of Medical rag, it is characterised in that it is any described natural anti-that described Medical rag contains claim 1-4 Fungus extract.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111074540A (en) * | 2019-11-21 | 2020-04-28 | 吴杨 | Antibacterial agent for textiles and preparation method thereof |
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| CN111074540A (en) * | 2019-11-21 | 2020-04-28 | 吴杨 | Antibacterial agent for textiles and preparation method thereof |
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