CN101884801A - Air freshener and preparation method and application thereof - Google Patents
Air freshener and preparation method and application thereof Download PDFInfo
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- CN101884801A CN101884801A CN2009100836008A CN200910083600A CN101884801A CN 101884801 A CN101884801 A CN 101884801A CN 2009100836008 A CN2009100836008 A CN 2009100836008A CN 200910083600 A CN200910083600 A CN 200910083600A CN 101884801 A CN101884801 A CN 101884801A
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- air freshener
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- quintessence oil
- tea tree
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- 238000002360 preparation method Methods 0.000 title abstract description 29
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Landscapes
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an air freshener and a preparation method and application thereof. The active ingredients of the air freshener comprise thyme essential oil, mint essential oil and tea tree oil. Bacteriostatic experiments prove the bacteriostatic effect of the air freshener, and the experimental results prove that the air freshener has the advantages of safety, high efficiency, no toxin, no irritation and environmental protection, has strong bacteriostatic and antibacterial effects, and can purify the air and improve the air quality. The air freshener can effectively suppress the activities of colibacillus and staphylococcus aureus in the air, has broad-spectrum antimicrobial activity, can play roles in completely improving the air quality and keeping indoor air fresh and clean, and has bacteriostatic activity similar to the streptomyces avermitilis. The air freshener is prepared from aromatic plant essential oil, such as lavender essential oil and rosemary essential oil instead of chemically synthesized essence, is non-toxic and harmless to human body, and has no irritating harm, sensitivity or other side effects.
Description
Technical field
The present invention relates to a kind of air freshener and preparation method thereof and application, specifically, the present invention relates to a kind of antibacterial type air freshener of forming by natural plant essential oils that suppresses common pathogen effect in the air that has.
Background technology
IAQ (indoor air quality) is most important to health, especially the outburst of SARS in 2003 more impels people more and more to pay close attention to the problem of microbial contamination in the room air, in addition, many public places are owing to the reason such as not smooth of ventilating can't be come the nature environment purification by fresh air, can accumulate abnormal flavour unavoidably, make the people produce uncomfortable feeling.Use has the sterilizing effect, have aromatic odor and simple, save trouble, the liquid spray air freshener is killed airborne pathogenic bacteria, drives away abnormal flavour, is improved main selection that air quality has just become people easily.
At present, the air freshener of selling on the market is of a great variety, odor type is different, but being equipped with essence again with organic compound such as aromatic compound and some monoterpenes chemical compounds etc. mostly mixes, come covering smell by fragrant, alleviate people to the abnormal flavour uncomfortable feeling, but can not really play the effect that purifies air, and wherein some synthetics life-time service can produce harm to human body, cause neurasthenia or upper respiratory tract system pathological changes, even to some immune systems, hemopoietic system has injury, as dimethylbenzene, ketone and aldehyde etc., heavy dose of use can cause poisoning.Other has report to point out that the air freshener of some chemosynthesis can bring out cancer.Therefore, the safety of air freshener and effectiveness thereof draw attention further.
At the problems referred to above, at present both at home and abroad research worker is invested natural plant air freshener field with research emphasis, the patent application of the air freshener that a lot of relevant natural plant extracts are prepared from also occurred.Publication number is to disclose a kind of All Pure Nature liquid type air freshener in the patent of CN1562380A, contain natural aromatic plant quintessence oil-Herba Lysimachiae foenum-graeci quintessence oil, Mentha arvensis L. syn.M.haplocalyxBrig quintessence oil and Fructus Citri Limoniae quintessence oil, and use thymic distilled water formulated as the carrier of air freshener.Publication number is that disclosing a kind of in the patent of CN 1436572A is the air freshener of main effective ingredient with the eucalyptus leaves extracting solution, with eucalyptus leaves water extract instead of chemical antibacterial, and solubilizing agent (Tweens or spans solubilizing agent or the mixture of the two) and antiseptic etc. have been added.Publication number is that the composition of air freshener of the patent disclosure of CN1286032 comprises rosemary ethereal oil, Ocimum Basilicum quintessence oil, Herba Schizonepetae quintessence oil, Herba Moslae quintessence oil, Benzoinum extractum, ethanol, deionized water and slow releasing agent, and this air freshener carries out disinfection to air with the form of stock solution or tablet.Publication number be CN 1342496A patent disclosure a kind of Chinese medicine spray for refreshing air and preparation method thereof, with Folium Artemisiae Argyi, Radix Isatidis, Flos Caryophylli, Cortex Acanthopancis, Borneolum Syntheticum, Herba Taraxaci etc. is raw material, each raw material is mixed the back according to a certain ratio pulverize, soak, filter, make spray.The patent of utilizing Chinese medicine ingredients to make air freshener also has the patent of publication number for CN101143226A, serves as main antibiotic and anthelmintic ingredient with Chinese medicine medical material Herba Artemisiae Annuae quintessence oil and eucalyptus leaf essential oil wherein, replaces the chemical antibacterial, worm-expelling agent of traditional toxic side effect.Publication number be CN10104486 patent disclosure a kind of spraying liquid that is used for environmental protection, diseases prevention, the mosquito of dispelling, belong to the Chinese medicine technical field of pharmaceuticals equally, this spraying is made up of Fructus Linderae Glaucae, Herba Menthae and a small amount of edible essence (as the edible essence of Fructus Ananadis comosi odor type or Fructus Citri tangerinae odor type), and indoor 22 kinds of antibacterials and 6 kinds of viruses can be eliminated and suppress to Chinese medicine ingredients wherein in the mosquito of dispelling rapidly.Publication number is to disclose and can directly aerosol have been sprayed in the air in the patent of CN 1088051A, can also be with natural medicinal formulations and the supporting use of air humidifier, ultrasonic atomizatio effect by humidifier is spread in the air air freshener, and environment is carried out disinfection.Publication number is the notion that discloses the multipurpose bacteriostatic refresher in the patent of CN1817369A, be with the harmful gas lyase masterbatch in the medium, natural peppermint essence oil content do not join spare matter in the environment-friendly type dissolvant white oil of carrier mass after, in the environment-friendly type dissolvant white oil that the anionic additive and the air catalyst catalyst of medium fully is added to the mixed carrier material, obtain multifunctional air bacteriostatic refresher again.
Generally speaking, use natural plant extracts on quality and quantity, all to be greatly improved in recent years with respect to traditional synthetic as the air freshener or the air purifying preparation of main component, but also exist as antibacterial, not remarkable, antibacterial time of antibacterial effect of air freshener not lastingly, abnormal smells from the patient is pleasant inadequately, the quintessence oil proportioning is improper and human body had problem such as stimulation.
Therefore how when keeping natural plant essential oils attribute (environmental protection, antibacterial, fragrance), to improve bacteriostatic scope and effect, and the air freshener of making is reduced to minimum even thoroughly eliminated still problem that is worth continuing further investigation the harm of human body.
Summary of the invention
In order to overcome the deficiency of existing air freshener, the present invention aims to provide a kind of antibacterial type air freshener of being made up of natural plant essential oils that suppresses common pathogen effect in the air that has.
Air freshener provided by the present invention, its active component comprises thyme oil, Herba Menthae quintessence oil and tea tree oil.
In the above-mentioned air freshener, the volume ratio of described thyme oil, described Herba Menthae quintessence oil and described tea tree oil is (3-10): (3-10): (3-8).
Better for the abnormal smells from the patient and the fungistatic effect that make described air freshener, also comprise Herba Lysimachiae foenum-graeci quintessence oil, rosemary ethereal oil and ethanol in the described air freshener.
In the above-mentioned air freshener, described thyme oil, described Herba Menthae quintessence oil, described tea tree oil, described Herba Lysimachiae foenum-graeci quintessence oil, described rosemary ethereal oil and described alcoholic acid volume ratio are (3-10): (3-10): (3-8): (5-10): (3-5): (10-30);
Described ethanol is that volumn concentration is 95% ethanol water.
During concrete the use, can water above-mentioned air freshener be carried out using after the dilution of certain multiple proportions, the volume ratio of described thyme oil, described Herba Menthae quintessence oil, described tea tree oil, described Herba Lysimachiae foenum-graeci quintessence oil, described rosemary ethereal oil, described second alcohol and water is (3-10): (3-10): (3-8): (5-10): (3-5): (10-30): (40-60).
In the above-mentioned air freshener, described various plants essential oils can directly extract from plant and obtain, and also can be commercially available products.The described method of extracting quintessence oil from plant is the method for this area routine.
In the above-mentioned air freshener, described thyme oil has intensive antibiotic and sterilizing characteristic, can assist body to resist disease, spreading of control antibacterial, immune system also there is certain benefit, nerve, activating brain cell be can also strengthen, human body memory and attention improved, the depression that can help people to improve low emotion, used-up sensation or defeat; Described Herba Menthae quintessence oil has the excellent antibiotic activity, and fragrance is refrigerant pleasant, strong, has penetrance, and the algefacient attribute can be pacified indignation, hysteria and frightened state, and to soul tired out and dejected emotion, effect is excellent; Described tea tree oil is the excellent antibacterial of respiratory tract, all the time because its significant bactericidal effect is subjected to the attention of domestic and international research worker, tea tree oil can to most of antibacterials 1% in addition littler concentration play inhibitory action; Described Herba Lysimachiae foenum-graeci quintessence oil fragrant odour uniqueness, the main chemical compositions linalool have pleasant fragrance of a flower fragrance, and linalool oxide has the sweet note of fragrance of a flower band, has the effect of calm the nerves Fructus Alpiniae Oxyphyllae and clean air, and inhibited to multiple mushroom; Described rosemary ethereal oil has the hot medicated incense fragrance of refrigerant point, give salubrious sense, and fragrance is strong, can memory reinforcing, and refreshment improves intense strain.
Air freshener of the present invention can be made various dosage forms, specifically can be spray.
Another object of the present invention provides the preparation method of above-mentioned air freshener.
This method may further comprise the steps;
(1) thyme oil, Herba Menthae quintessence oil, tea tree oil, Herba Lysimachiae foenum-graeci quintessence oil and rosemary ethereal oil are mixed, obtain blend of essential oils;
(2) in the blend of essential oils of above-mentioned steps (1), add the second alcohol and water, obtain air freshener.
Air freshener of the present invention can be used for suppressing airborne pathogenic bacterium.
Described pathogenic bacterium comprise escherichia coli, staphylococcus aureus and bacillus pyocyaneus etc.
Compare with existing air freshener on the market, that air freshener of the present invention has is safe, efficient, nontoxic, the advantage of nonirritant and environmental protection.Because plants essential oil has very strong volatility, thereby have stronger antibacterial, an antibiotic effect, can purify air, improve air quality, simultaneously because the various health-care effecies of natural plant essential oils, people are worked at psychological aspects after sucking fragrance, make the people feel pleasant, joyful, peaceful, the effect of " aromatotherapy " at present all the fashion just also can be embodied in air freshener of the present invention.Air freshener of the present invention can effectively suppress conditioned pathogen common in the air, as escherichia coli, staphylococcus glucose coccus etc., has broad spectrum antibiotic activity, can play the thorough air quality of improving, the effect of cleaning with fresh air in the holding chamber, its bacteriostatic activity is similar to streptomycete, air freshener of the present invention aromatic essential oil (Herba Lysimachiae foenum-graeci quintessence oil, rosemary ethereal oil) the synthetic essence of instead of chemical, the pleasant abnormal smells from the patient of its delicate fragrance can make the people feel to place oneself in the midst of seemingly among the nature, people's body and mind is loosened, spirit is brisk and neat, relaxed and joyful, nontoxic when plants essential oil is in full play to human body, do not stimulate side effect such as injury and sensitivity yet.
The specific embodiment
Experimental technique described in the following embodiment if no special instructions, is conventional method; Described reagent and biomaterial if no special instructions, all can obtain from commercial channels.
Thyme oil available from Rushan City, Shandong natural plant essential oils development corporation, Ltd., Herba Menthae quintessence oil available from 69 of farming 4 teachers of the Production and Construction Corps of Xinjiang, tea tree oil available from Nanning City's ten thousand tame brightness plantation company limiteies, Herba Lysimachiae foenum-graeci quintessence oil available from 69 of farming 4 teachers of the Production and Construction Corps of Xinjiang, rosemary ethereal oil available from 69 of farming 4 teachers of the Production and Construction Corps of Xinjiang.
The preparation of embodiment 1, air freshener and effect experiment thereof
1, the preparation of air freshener
(1) 5ml thyme oil, 5ml Herba Menthae quintessence oil, 8ml tea tree oil, 10ml Herba Lysimachiae foenum-graeci quintessence oil and 3ml rosemary ethereal oil are joined in the container that agitator is installed, stir.
(2) the ethanol 29ml and the deionized water 40ml of adding 95% in the mixture of above-mentioned steps (1) stir and promptly obtain the 100ml air freshener.
The air freshener of above-mentioned preparation need not pressurization, can be in the spray bottle of directly packing into to the room air sterilization that carries out disinfection, and simple to operate, greatly simplified the production operation program, applied widely.
2, the effect of air freshener experiment
With the bacteriostasis antibiosis effect that among the QB/T 2738-2005 (" People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ") examination criteria of escherichia coli (Escherichia coli), staphylococcus aureus (Staphylococcusaureus) and bacillus pyocyaneus (Pseudomonas.aeruginosa) is detected the air freshener of above-mentioned steps 1 preparation, to understand the antibacterial bacteriostatic activity of air freshener of the present invention, comprise antibacterial circle diameter and antibacterial time etc., concrete detection method is as follows:
(1) effect of antibacterial around-France detection air freshener
With reference to QB/T 2738-2005, the fungistatic effect method of inspection of 7.5 antibacterial type daily chemical products (antibacterial around-France) detects the fungistatic effect of the air freshener of above-mentioned steps 1 preparation.
Get aseptic dry filter paper sheet, every air freshener that drips 1 preparation of 20ul above-mentioned steps, simultaneously with the filter paper that drips the equal volume sterile distilled water and drip 20ul concentration be 1mg/ml streptomycin filter paper in contrast.Above-mentioned filter paper is lain against respectively in the aseptic plate of cleaning, uncapping, it is standby behind the natural drying to place under calorstat (37 ℃) oven dry or the room temperature.
Drawing concentration respectively with the pipettor of sterilization is 1 * 10
5Cfu/ml-9 * 10
5Each 100ul of bacteria suspension of the escherichia coli of cfU/ml (E.coli) ATCC 11229, the golden yellow subspecies ATCC 6538 of staphylococcus aureus (S.aureus) and bacillus pyocyaneus (P.aeruginosa) ATCC 15442 evenly smears 3 times with spreading rod at the nutrient agar planar surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last.Build plate, put under the room temperature condition and cultivate.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of air freshener, streptomycin or sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up and pastes the filter paper that the filter paper putting 4 droppings air freshener is arranged, 1 filter paper of submitting streptomycin and 1 dropping have sterilized water.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting,, make it be close to planar surface with the light filter pressing scraps of paper of aseptic nipper.Build plate, place 37 ℃ of calorstats, observed result behind the cultivation 12h-24h.
With the diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get three multiple meansigma methodss as experimental result.Inhibition zone diameter belongs to B2 higher level (bacteriostasis rate is the B1 level 90% above person, and bacteriostasis rate is the B2 level) greater than 7mm person between 50%～90%; Inhibition zone diameter is less than or equal to 7mm person, belongs to B3 with subordinate (bacteriostasis rate is the B3 level 50% following person).Time dependent result is as shown in table 1 for antibacterial circle diameter.Wherein, a1, a2, a3 represent and drip sterile distilled water, drip the antibacterial circle diameter measurement result of streptomycin and dropping air freshener respectively.
The time dependent result of table 1 antibacterial circle diameter
(2) by adding up the effect of clump count detection air freshener
Measure the clump count on the flat board that posting of above-mentioned steps (1) drip the filter paper that air freshener is arranged respectively, calculate sterilizing rate.Three repetitions are established in experiment, and the result keeps integer, and the result of sterilizing rate is as shown in table 2.
Sterilizing rate (%)=(I-II)/I * 100%,
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of air freshener.
According to the QB/T 2738-2005 (regulation in " People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ", sterilizing rate 〉=90% belongs to the A1 level, sterilizing rate belongs to the A2 level between 50%～90%, sterilizing rate≤50% belongs to the A3 level.
The bactericidal effect of table 2 air freshener
The preparation of embodiment 2, air freshener and effect experiment thereof
1, the preparation of air freshener
(1) 10ml thyme oil, 3ml Herba Menthae quintessence oil, 5ml tea tree oil, 10ml Herba Lysimachiae foenum-graeci quintessence oil and 5ml rosemary ethereal oil are joined in the container that agitator is installed, stir.
(2) the ethanol 17ml and the deionized water 50ml of adding 95% in the mixture of above-mentioned steps (1) stir and promptly obtain the 100ml air freshener.
The air freshener of above-mentioned preparation need not pressurization, can be in the spray bottle of directly packing into to the room air sterilization that carries out disinfection, and simple to operate, greatly simplified the production operation program, applied widely.
2, the effect of air freshener experiment
With the bacteriostasis antibiosis effect that among the QB/T 2738-2005 (" People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ") examination criteria of escherichia coli (Escherichia coli), staphylococcus aureus (Staphylococcusaureus) and bacillus pyocyaneus (Pseudomonas.aeruginosa) is detected the air freshener of above-mentioned steps 1 preparation, to understand the antibacterial bacteriostatic activity of air freshener of the present invention, comprise antibacterial circle diameter and antibacterial time etc., concrete detection method is as follows:
(1) effect of antibacterial around-France detection air freshener
With reference to QB/T 2738-2005, the fungistatic effect method of inspection of 7.5 antibacterial type daily chemical products (antibacterial around-France) detects the fungistatic effect of the air freshener of above-mentioned steps 1 preparation.
Get aseptic dry filter paper sheet, every air freshener that drips 1 preparation of 20ul above-mentioned steps, simultaneously with the filter paper that drips the equal volume sterile distilled water and drip 20ul concentration be 1mg/ml streptomycin filter paper in contrast.Above-mentioned filter paper is lain against respectively in the aseptic plate of cleaning, uncapping, it is standby behind the natural drying to place under calorstat (37 ℃) oven dry or the room temperature.
Drawing concentration respectively with the pipettor of sterilization is 1 * 10
5Cfu/ml-9 * 10
5Each 100ul of bacteria suspension of the escherichia coli of cfU/ml (E.coli) ATCC 11229, the golden yellow subspecies ATCC 6538 of staphylococcus aureus (S.aureus) and bacillus pyocyaneus (P.aeruginosa) ATCC 15442 evenly smears 3 times with spreading rod at the nutrient agar planar surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last.Build plate, put under the room temperature condition and cultivate.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of air freshener, streptomycin or sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up and pastes the filter paper that the filter paper putting 4 droppings air freshener is arranged, 1 filter paper of submitting streptomycin and 1 dropping have sterilized water.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting,, make it be close to planar surface with the light filter pressing scraps of paper of aseptic nipper.Build plate, place 37 ℃ of calorstats, observed result behind the cultivation 12h-24h.
With the diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get three multiple meansigma methodss as experimental result.Inhibition zone diameter belongs to the B2 higher level greater than 7mm person, and bacteriostasis rate is the B1 level 90% above person, and bacteriostasis rate is the B2 level between 50%～90%; Inhibition zone diameter is less than or equal to 7mm person, belongs to B3 with subordinate, and bacteriostasis rate is the B3 level 50% following person.Time dependent result is as shown in table 3 for antibacterial circle diameter.Wherein, b1, b2, b3 represent and drip sterile distilled water, drip the antibacterial circle diameter measurement result of streptomycin and dropping air freshener respectively.
The time dependent result of table 3 antibacterial circle diameter
(2) by adding up the effect of clump count detection air freshener
Measure the clump count on the flat board that posting of above-mentioned steps (1) drip the filter paper that air freshener is arranged respectively, calculate sterilizing rate.Three repetitions are established in experiment, and the result keeps integer, and the result of sterilizing rate is as shown in table 4.
Sterilizing rate (%)=(I-II)/I * 100%,
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of air freshener.
According to the QB/T 2738-2005 (regulation in " People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ", sterilizing rate 〉=90% belongs to the A1 level, sterilizing rate belongs to the A2 level between 50%～90%, sterilizing rate≤50% belongs to the A3 level.
The bactericidal effect of table 4 air freshener
The preparation of embodiment 3, air freshener and effect experiment thereof
1, the preparation of air freshener
(1) 8ml thyme oil, 10ml Herba Menthae quintessence oil, 3ml tea tree oil, 8ml Herba Lysimachiae foenum-graeci quintessence oil and 4ml rosemary ethereal oil are joined in the container that agitator is installed, stir.
(2) the ethanol 12ml and the deionized water 55ml of adding 95% in the mixture of above-mentioned steps (1) stir and promptly obtain the 100ml air freshener.
The air freshener of above-mentioned preparation need not pressurization, can be in the spray bottle of directly packing into to the room air sterilization that carries out disinfection, and simple to operate, greatly simplified the production operation program, applied widely.
2, the effect of air freshener experiment
With the bacteriostasis antibiosis effect that among the QB/T 2738-2005 (" People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ") examination criteria of escherichia coli (Escherichia coli), staphylococcus aureus (Staphylococcusaureus) and bacillus pyocyaneus (Pseudomonas.aeruginosa) is detected the air freshener of above-mentioned steps 1 preparation, to understand the antibacterial bacteriostatic activity of air freshener of the present invention, comprise antibacterial circle diameter and antibacterial time etc., concrete detection method is as follows:
(1) effect of antibacterial around-France detection air freshener
With reference to QB/T 2738-2005, the fungistatic effect method of inspection of 7.5 antibacterial type daily chemical products (antibacterial around-France) detects the fungistatic effect of the air freshener of above-mentioned steps 1 preparation.
Get aseptic dry filter paper sheet, every air freshener that drips 1 preparation of 20ul above-mentioned steps, simultaneously with the filter paper that drips the equal volume sterile distilled water and drip 20ul concentration be 1mg/ml streptomycin filter paper in contrast.Above-mentioned filter paper is lain against respectively in the aseptic plate of cleaning, uncapping, it is standby behind the natural drying to place under calorstat (37 ℃) oven dry or the room temperature.
Drawing concentration respectively with the pipettor of sterilization is 1 * 10
5Cfu/ml-9 * 10
5Each 100ul of bacteria suspension of the escherichia coli of cfU/ml (E.coli) ATCC 11229, the golden yellow subspecies ATCC 6538 of staphylococcus aureus (S.aureus) and bacillus pyocyaneus (P.aeruginosa) ATCC 15442 evenly smears 3 times with spreading rod at the nutrient agar planar surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last.Build plate, put under the room temperature condition and cultivate.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of air freshener, streptomycin or sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up and pastes the filter paper that the filter paper putting 4 droppings air freshener is arranged, 1 filter paper of submitting streptomycin and 1 dropping have sterilized water.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting,, make it be close to planar surface with the light filter pressing scraps of paper of aseptic nipper.Build plate, place 37 ℃ of calorstats, observed result behind the cultivation 12h-24h.
With the diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get three multiple meansigma methodss as experimental result.Inhibition zone diameter belongs to the B2 higher level greater than 7mm person, and bacteriostasis rate is the B1 level 90% above person, and bacteriostasis rate is the B2 level between 50%～90%; Inhibition zone diameter is less than or equal to 7mm person, belongs to B3 with subordinate, and bacteriostasis rate is the B3 level 50% following person.Time dependent result is as shown in table 5 for antibacterial circle diameter.Wherein, c1, c2, c3 represent and drip sterile distilled water, drip the antibacterial circle diameter measurement result of streptomycin and dropping air freshener respectively.
The time dependent result of table 5 antibacterial circle diameter
(2) by adding up the effect of clump count detection air freshener
Measure the clump count on the flat board that posting of above-mentioned steps (1) drip the filter paper that air freshener is arranged respectively, calculate sterilizing rate.Three repetitions are established in experiment, and the result keeps integer, and the result of sterilizing rate is as shown in table 6.
Sterilizing rate (%)=(I-II)/I * 100%,
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of air freshener.
According to the QB/T 2738-2005 (regulation in " People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ", sterilizing rate 〉=90% belongs to the A1 level, sterilizing rate belongs to the A2 level between 50%～90%, sterilizing rate≤50% belongs to the A3 level.
The bactericidal effect of table 6 air freshener
The mode spraying effect of air freshener employing spraying of the present invention can be more directly perceived, and fungistatic effect also can be more obvious.Also there are some restrictions in the assay method of above-mentioned antibacterial ring, can have influence on evaluation such as air freshener diffusivity on flat board etc. to fungistatic effect, this also is the fungistatic effect reason poor slightly than streptomycin of air freshener in the foregoing description 1,2,3, but the air freshener of the present invention preparation all reaches more than 80% the sterilizing rate of escherichia coli, staphylococcus aureus and bacillus pyocyaneus.
Claims (10)
1. air freshener, its active component comprises thyme oil, Herba Menthae quintessence oil and tea tree oil.
2. air freshener according to claim 1 is characterized in that: the volume ratio of described thyme oil, described Herba Menthae quintessence oil and described tea tree oil is (3-10): (3-10): (3-8).
3. air freshener according to claim 1 is characterized in that: also comprise Herba Lysimachiae foenum-graeci quintessence oil, rosemary ethereal oil and ethanol in the described air freshener.
4. air freshener according to claim 3 is characterized in that: described thyme oil, described Herba Menthae quintessence oil, described tea tree oil, described Herba Lysimachiae foenum-graeci quintessence oil, described rosemary ethereal oil and described alcoholic acid volume ratio are (3-10): (3-10): (3-8): (5-10): (3-5): (10-30).
5. air freshener according to claim 3 is characterized in that: described ethanol is that volumn concentration is 95% ethanol water.
6. air freshener according to claim 3 is characterized in that: described air freshener is made up of thyme oil, Herba Menthae quintessence oil, tea tree oil, Herba Lysimachiae foenum-graeci quintessence oil, rosemary ethereal oil, second alcohol and water.
7. air freshener according to claim 6 is characterized in that: the volume ratio of described thyme oil, described Herba Menthae quintessence oil, described tea tree oil, described Herba Lysimachiae foenum-graeci quintessence oil, described rosemary ethereal oil, described second alcohol and water is (3-10): (3-10): (3-8): (5-10): (3-5): (10-30): (40-60).
8. a method for preparing the described air freshener of claim 6 may further comprise the steps:
(1) thyme oil, Herba Menthae quintessence oil, tea tree oil, Herba Lysimachiae foenum-graeci quintessence oil and rosemary ethereal oil are mixed, obtain blend of essential oils;
(2) in the blend of essential oils of above-mentioned steps (1), add the second alcohol and water, obtain air freshener.
9. arbitrary described air freshener application in the pathogenic bacterium in suppressing air among the claim 1-7.
10. application according to claim 9 is characterized in that: described pathogenic bacterium comprise escherichia coli, staphylococcus aureus and bacillus pyocyaneus.
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