CN103341196A - Bacteriostatic air-refreshing composition and its preparation method and use - Google Patents
Bacteriostatic air-refreshing composition and its preparation method and use Download PDFInfo
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Abstract
The invention relates to a bacteriostatic air-refreshing composition and its preparation method and use. The bacteriostatic air-refreshing composition comprises, by volume, 70-95% of a solvent and 5-30% of plant essential oil, wherein the plant essential oil is cinnamon essential oil and the solvent is ethanol. The bacteriostatic air-refreshing composition has effects of refreshing air and inhibiting common pathogenic bacteria in air.
Description
Technical field
The present invention relates to a kind of composition and method of making the same and purposes of antibacterial type fresh air.
Background technology
IAQ (indoor air quality) is most important to health, especially SARS in 2003, the outburst of H5N1 bird flu in 2004 and H7N9 bird flu in 2013 more impels people to pay close attention to the problem of indoor microbial contamination, and many public places are owing to the reason such as not smooth of ventilating can't come nature to purify air by fresh air, can stockpile abnormal flavour unavoidably, and 2012 the year end and 2013 at the beginning of Beijing PM2.5 and the haze weather reported, dust wherein and granule carry many microorganisms, can cause respiratory tract infection and pathophoresis, make the people produce uncomfortable feeling.
The antibacterial size by unicellular schizogamy, is doubled and redoubled about 0.5-1 μ m.In the place of indoor humidity, the relative humidity of environment is easy growth and breeding when 90%--100%.
The antibacterial that occurs in the urban ecological system air has 21 and belongs to, gram positive bacteria is more, gram negative bacteria is less, wherein superiority bacteria spp is bacillus (Bacillus), staphylococcus (Staphylococcus), Microbacterium (Microbacterium) and Micrococcus (Micrococcus)
The fungus size is unicellular or many cells mycelia elongation propagation about about 3～100 μ m.When the fungus spore is attached to nutritious source, the place that air is arranged, and temperature at 20-35 ℃ of left and right sides relative humidity in 75%-100%, will growth and breeding, the mycelia elongation, growth and maturity also discharge spore, pollute room air, and fungus also can give out special foul smell in prolific process.In indoor humidity, the place of dewfall or the place that is subjected to the water enchroachment (invasion) evil, as the kitchen, in bathroom and the toilet, the relative humidity of environment is up to indoor construction material and the equipment of 90%-100%, and just inevitable multiplying easily is fungus, and this is a ubiquitous problem.
Fungal spore can cause human fungal disease in the air, respiratory especially, skin allergic disease and sick Building syndrome etc.The increase of air fungal spore concentration reduces the human body pulmonary function, increases respiratory disease and chronic lung disease and ill probabilities such as cardiovascular disease and pulmonary carcinoma such as flu, rapid breathing, asthma.Airborne Alternaria, aspergillus and Cladosporium bacterium etc. are the modal funguses that can cause people's skin allergy.
Air microbe pollutes and causes the case of health influence to happen occasionally, even threat to life is not rare yet.Pure air is to cut off effective, the most direct method in respiratory infectious disease route of transmission.Have pure and fresh air, can lower fatigue to a certain extent, lax nervous emotion is alleviated the work living pressure, is of value to the physical and mental health of human body.
At present, the air aromatic of selling on the market is of a great variety, odor type is different, mixes but be equipped with essence again with organic compound such as aromatic compound and monoterpenes chemical compound etc. mostly, comes covering smell by fragrant, alleviate people to the uncomfortable sensation of abnormal flavour, but can not really play the effect that purifies air, and wherein the chemical compound life-time service can produce harm to human body, and household articles is caused oxide etch, and human eye and respiratory tract organ damaged, seriously can be carcinogenic.So the biocidal property of air aromatic, the feature of environmental protection and mind ﹠ body care draw attention further.
At the problems referred to above, the research and development scholar invests natural air aromatic field with research emphasis both at home and abroad at present, the patent application that a lot of relevant natural extracts are prepared from the air aromatic also occurred.Use natural plant extracts all to be enhanced with cleanser relative traditional chemical composite on quality and quantity as the air freshener of main component, but also there is the medicinal plants quintessence oil exploitation limitation that is used for air aromatic field, the composite proportioning of medicinal quintessence oil is improper, antibacterial, antibacterial effect is not remarkable, the antibacterial time is not lasting
How to carry out effective pure air, reduce because air microbe pollutes the infection that causes is the major issue that medical circle is paid close attention to always.Therefore, be badly in need of also will having fungistatic effect when exploitation has natural faint scent, safety and environmental protection attribute quintessence oil and give prominence to, longer duration, the medicinal plants quintessence oil of disease-prevention health attribute is as the pure natural air aromatic of main component.
Summary of the invention
Technical problem to be solved by this invention provides a kind of composition and method of making the same and purposes of antibacterial type fresh air, and this compositions has fresh air, suppresses the effect of common pathogen in the air, to the oidiomycetic killing rate of white more than 95%.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of compositions of antibacterial type fresh air, in the percentage ratio of raw material cumulative volume, formed by following raw material: solvent 70%-95%, plants essential oil 5%-30%, wherein, described plants essential oil is cinnamon essential oil, and described solvent is ethanol.
On the basis of technique scheme, the present invention can also do following improvement.
Further, described concentration of alcohol is 95%.
Further, formed by following raw material: cinnamon essential oil 25ml, 95% ethanol 75ml.
Further, formed by following raw material: cinnamon essential oil 10ml, 95% ethanol 90ml.
Further, formed by following raw material: cinnamon essential oil 5ml, 95% alcohol 95 ml.
The present invention also provides a kind of preparation of compositions method of antibacterial type fresh air, comprising:
1) adopts this area conventional method from plant, to extract cinnamon essential oil, or buy commercially available cinnamon essential oil product.
2) with cinnamon essential oil and etoh solvent mix homogeneously, namely.
The present composition can be made into spray, directly sends use forth or cooperates ultrasonic atomizatio to use with humidifier.
The compositions of antibacterial type fresh air is for the preparation of air freshener as mentioned above, is used for suppressing in air escherichia coli, staphylococcus aureus, the Candida albicans one or more.
The invention has the beneficial effects as follows:
The cinnamon essential oil that the compositions of antibacterial type fresh air of the present invention adopts has very strong antibiotic and bactericidal properties to microorganism, pacify people excellently because that operating pressure or life constrain that face causes is drained and weak, heart is dejected, quick-witted tired good, can also increase self-confidence; Energy reinforcement respiratory tract alleviates the respiratory tract difficulty, and treatment flu or respiratory tract infection alleviate cold symptoms, helps the people to keep clear-headed.In addition, also have antitumor, antiulcer, analgesic, the multiple effects such as spasmolytic, parasite killing of easing pain.Be used as antiseptic or spice in industries such as food and article of everyday use, the present invention is as the active component that suppresses air microbe in the air freshener always.Therefore, present composition fungistatic effect is outstanding, disease-prevention health, natural faint scent, safety and environmental protection.
The specific embodiment
Below principle of the present invention and feature are described, institute only gives an actual example and to be used for explaining the present invention, is not for restriction scope of the present invention.
Below the present invention among the embodiment used cinnamon essential oil provided by Shenzhen virtue profit Science and Technology Ltd..
Below the present invention among the embodiment used escherichia coli (E.coil) 8099, the golden yellow subspecies ATCC6538 of staphylococcus aureus (S.aureus) and Candida albicans all buy the Disease Control and Prevention Center from Beijing.
Embodiment 1 antibacterial type fresh air preparation of compositions and effect experiment thereof
1, preparation of compositions: the 25ml cinnamon essential oil is added 75ml95% ethanol, stir, namely get the compositions of 100ml antibacterial type fresh air.
Above-mentioned antibacterial type fresh air compositions need not pressurization, can be to the room air sterilization that carries out disinfection in the aerosol can of directly packing into, and simple to operate, simplified the production operation program greatly, applied widely.
The compositions of above-mentioned antibacterial type fresh air, also available humidifier ultrasonic atomizatio is disseminated to the sterilization that carries out disinfection in the room air, and is simple to operate, simplified the production operation program greatly, applied widely.
2, the effect of antibacterial type fresh air compositions experiment
With QB/T2738-2005(" People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ") in the touchstone of escherichia coli (Escherichia coli), staphylococcus aureus (Staphyloccocus aureus) is surveyed the bacteriostasis antibiosis effect of the antibacterial type fresh air compositions of above-mentioned steps 1 preparation, to understand the bacteriostasis antibiosis activity of antibacterial type fresh air compositions of the present invention, comprise antibacterial circle diameter and antibacterial time etc., concrete detection method is as follows:
1) fungistatic effect of antibacterial around-France detection antibacterial type air compositions
With reference to QB/T2738-2005, the method for inspection of the fungistatic effect of 7.5 antibacterial type daily chemical products (antibacterial around-France) detects the bacteriostasis antibiosis effect of the antibacterial type fresh air compositions of above-mentioned steps 1 preparation.
Cut-off directly is the aseptic dry filter paper sheet of 5mm, and every drips the antibacterial type delicate fragrance air compositions that makes among the 20 μ L above-mentioned 1, simultaneously with drip with the sterilized water distilled water filter paper of volume and drip 20 μ L concentration be the 1mg/ml streptomycin filter paper in contrast.Above-mentioned filter paper is lain against respectively in the clean aseptic plate, uncap place dry under calorstat (37 ℃) oven dry or the room temperature afterwards standby.
Drawing concentration respectively with aseptic pipettor is 1x10
5Cfu/ml-9x10
5Each 100 μ L of the bacteria suspension of the escherichia coli of cfu/ml (E.coil) 8099 and the golden yellow subspecies ATCC6538 of staphylococcus aureus (S.aureus) evenly are coated with 3 times with spreading rod on the nutrient agar panel surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last, build plate, put under the room temperature condition and cultivate, standby.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of antibacterial type fresh air compositions, streptomycin or sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up and pastes the filter paper putting 4 droppings air freshener is arranged, 1 dropping the filter paper of streptomycin and the filter paper that 1 dropping has sterilized water are arranged.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting, with the light filter pressing scraps of paper of aseptic nipper, make it be close to planar surface.Build plate, place 37 ℃ of calorstats, observed result behind the cultivation 12h-24h.
With diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get the meansigma methods of three repetitions as experimental result.Inhibition zone diameter is greater than 7mm person, and (bacteriostasis rate is the B1 level 90% above person to belong to the B2 higher level; Bacteriostasis rate is the B2 level between 50%～90%); Inhibition zone diameter is less than or equal to 7mm person, belongs to B3 with subordinate (bacteriostasis rate is the B3 level 50% following person).Antibacterial circle diameter result of variations in time is as shown in table 1, a1 wherein, and a2, a3 represent the dropping sterile distilled water respectively, drip streptomycin and drip the antibacterial circle diameter measurement result of antibacterial type fresh air compositions.
Table 1 antibacterial circle diameter is result of variations in time
2) by adding up the effect of clump count detection antibacterial type fresh air compositions
Measure above-mentioned steps 1 respectively) the flat board that posts the filter paper that drips antibacterial type fresh air compositions on clump count, calculate sterilizing rate, the experiment triplicate, the result keeps integer, the result of sterilizing rate is as shown in table 2.
Sterilizing rate (%)=(I-II)/I * 100%
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of antibacterial type fresh air compositions.
According to the regulation among the QB/T2738-2005 " People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ", sterilizing rate 〉=90% belongs to the A1 level, sterilizing rate belongs to the A2 level between 50%～90%, sterilizing rate≤50% belongs to the A3 level.
Table 2 antibacterial type fresh air compositions bactericidal effect
Embodiment 2 antibacterial type fresh air preparation of compositions and effect experiment thereof
1, preparation of compositions: the 10ml cinnamon essential oil is added 90ml95% ethanol, stir, namely get 100ml antibacterial type fresh air compositions.
Above-mentioned antibacterial type fresh air compositions need not pressurization, can be to the room air sterilization that carries out disinfection in the aerosol can of directly packing into, and simple to operate, simplified the production operation program greatly, applied widely.
Above-mentioned antibacterial type fresh air compositions, also available humidifier ultrasonic atomizatio is disseminated to the sterilization that carries out disinfection in the room air, and is simple to operate, simplified the production operation program greatly, applied widely.
2, the effect of antibacterial type fresh air compositions experiment
With QB/T2738-2005(" People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ") in the touchstone of escherichia coli (Escherichia coli), staphylococcus aureus (Staphyloccocus aureus) is surveyed the bacteriostasis antibiosis effect of the antibacterial type fresh air compositions of above-mentioned steps 1 preparation, to understand the bacteriostasis antibiosis activity of antibacterial type fresh air compositions of the present invention, comprise antibacterial circle diameter and antibacterial time etc., concrete detection method is as follows:
1) fungistatic effect of antibacterial around-France detection antibacterial type air compositions
With reference to QB/T2738-2005, the method for inspection of the fungistatic effect of 7.5 antibacterial type daily chemical products (antibacterial around-France) detects the bacteriostasis antibiosis effect of the antibacterial type fresh air compositions of above-mentioned steps 1 preparation.
Cut-off directly is the aseptic dry filter paper sheet of 5mm, and every drips the antibacterial type delicate fragrance air compositions that makes among the 20 μ L above-mentioned 1, simultaneously with drip with the sterilized water distilled water filter paper of volume and drip 20 μ L concentration be the 1mg/ml streptomycin filter paper in contrast.Above-mentioned filter paper is lain against respectively in the clean aseptic plate, uncap place dry under calorstat (37 ℃) oven dry or the room temperature afterwards standby.
Drawing concentration respectively with aseptic pipettor is 1x10
5Cfu/ml-9x10
5Each 100 μ L of the bacteria suspension of the escherichia coli of cfu/ml (E.coil) 8099 and the golden yellow subspecies ATCC6538 of staphylococcus aureus (S.aureus) evenly are coated with 3 times with spreading rod on the nutrient agar panel surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last, build plate, put under the room temperature condition and cultivate, standby.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of antibacterial type fresh air compositions, streptomycin or sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up and pastes the filter paper putting 4 droppings air freshener is arranged, 1 dropping the filter paper of streptomycin and the filter paper that 1 dropping has sterilized water are arranged.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting, with the light filter pressing scraps of paper of aseptic nipper, make it be close to planar surface.Build plate, place 37 ℃ of calorstats, observed result behind the cultivation 12h-24h.
With diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get the meansigma methods of three repetitions as experimental result.Inhibition zone diameter is greater than 7mm person, and (bacteriostasis rate is the B1 level 90% above person to belong to the B2 higher level; Bacteriostasis rate is the B2 level between 50%～90%); Inhibition zone diameter is less than or equal to 7mm person, belongs to B3 with subordinate (bacteriostasis rate is the B3 level 50% following person).Antibacterial circle diameter result of variations in time is as shown in table 3, b1 wherein, and b2, b3 represent the dropping sterile distilled water respectively, drip streptomycin and drip the antibacterial circle diameter measurement result of antibacterial type fresh air compositions.
Table 3 antibacterial circle diameter is result of variations in time
2) by adding up the effect of clump count detection antibacterial type fresh air compositions
Measure above-mentioned steps 1 respectively) the flat board that posts the filter paper that drips antibacterial type fresh air compositions on clump count, calculate sterilizing rate, the experiment triplicate, the result keeps integer, the result of sterilizing rate is as shown in table 4.
Sterilizing rate (%)=(I-II)/I * 100%
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of antibacterial type fresh air compositions.
According to the regulation among the QB/T2738-2005 " People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ", sterilizing rate 〉=90% belongs to the A1 level, sterilizing rate belongs to the A2 level between 50%～90%, sterilizing rate≤50% belongs to the A3 level.
The bactericidal effect of table 4 antibacterial type fresh air compositions
Embodiment 3 antibacterial type fresh air preparation of compositions and effect experiment thereof
1, preparation of compositions: the 5ml cinnamon essential oil is added 95ml95% ethanol, stir, namely get 100ml antibacterial type fresh air compositions.
Above-mentioned antibacterial type fresh air compositions need not pressurization, can be to the room air sterilization that carries out disinfection in the aerosol can of directly packing into, and simple to operate, simplified the production operation program greatly, applied widely.
Above-mentioned antibacterial type fresh air compositions, also available humidifier ultrasonic atomizatio is disseminated to the sterilization that carries out disinfection in the room air, and is simple to operate, simplified the production operation program greatly, applied widely.
2, the effect of antibacterial type fresh air compositions experiment
With QB/T2738-2005(" People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ") in the touchstone of escherichia coli (Escherichia coli), staphylococcus aureus (Staphyloccocus aureus) is surveyed the bacteriostasis antibiosis effect of the antibacterial type fresh air compositions of above-mentioned steps 1 preparation, to understand the bacteriostasis antibiosis activity of antibacterial type fresh air compositions of the present invention, comprise antibacterial circle diameter and antibacterial time etc., concrete detection method is as follows:
1) fungistatic effect of antibacterial around-France detection antibacterial type air compositions
With reference to QB/T2738-2005, the method for inspection of the fungistatic effect of 7.5 antibacterial type daily chemical products (antibacterial around-France) detects the bacteriostasis antibiosis effect of the antibacterial type fresh air compositions of above-mentioned steps 1 preparation.
Cut-off directly is the aseptic dry filter paper sheet of 5mm, and every drips the antibacterial type delicate fragrance air compositions that makes among the 20 μ L above-mentioned 1, simultaneously with drip with the sterilized water distilled water filter paper of volume and drip 20 μ L concentration be the 1mg/ml streptomycin filter paper in contrast.Above-mentioned filter paper is lain against respectively in the clean aseptic plate, uncap place dry under calorstat (37 ℃) oven dry or the room temperature afterwards standby.
Drawing concentration respectively with aseptic pipettor is 1x10
5Cfu/ml-9x10
5Each 100 μ L of the bacteria suspension of the escherichia coli of cfu/ml (E.coil) 8099 and the golden yellow subspecies ATCC6538 of staphylococcus aureus (S.aureus) evenly are coated with 3 times with spreading rod on the nutrient agar panel surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last, build plate, put under the room temperature condition and cultivate, standby.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of antibacterial type fresh air compositions, streptomycin or sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up and pastes the filter paper putting 4 droppings air freshener is arranged, 1 dropping the filter paper of streptomycin and the filter paper that 1 dropping has sterilized water are arranged.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting, with the light filter pressing scraps of paper of aseptic nipper, make it be close to planar surface.Build plate, place 37 ℃ of calorstats, observed result behind the cultivation 12h-24h.
With diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get the meansigma methods of three repetitions as experimental result.Inhibition zone diameter is greater than 7mm person, and (bacteriostasis rate is the B1 level 90% above person to belong to the B2 higher level; Bacteriostasis rate is the B2 level between 50%～90%); Inhibition zone diameter is less than or equal to 7mm person, belongs to B3 with subordinate (bacteriostasis rate is the B3 level 50% following person).Antibacterial circle diameter result of variations in time is as shown in table 5, c1 wherein, and c2, c3 represent the dropping sterile distilled water respectively, drip streptomycin and drip the antibacterial circle diameter measurement result of antibacterial type fresh air compositions.
Table 5 antibacterial circle diameter is result of variations in time
2) by adding up the effect of clump count detection antibacterial type fresh air compositions
Measure above-mentioned steps 1 respectively) the flat board that posts the filter paper that drips antibacterial type fresh air compositions on clump count, calculate sterilizing rate, the experiment triplicate, the result keeps integer, the result of sterilizing rate is as shown in table 6.
Sterilizing rate (%)=(I-II)/I * 100%
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of antibacterial type fresh air compositions.
According to the regulation among the QB/T2738-2005 " People's Republic of China's light industry standard-daily chemical products antibacterial bacteriostatic Evaluation on effect method ", sterilizing rate 〉=90% belongs to the A1 level, sterilizing rate belongs to the A2 level between 50%～90%, sterilizing rate≤50% belongs to the A3 level.
The bactericidal effect of table 6 antibacterial type fresh air compositions
Antibacterial type fresh air compositions employing spray pattern of the present invention and humidifier ultrasonic atomizatio mode effect can be more directly perceived, and fungistatic effect also can be more obvious.Also there are some restrictions in above-mentioned antibacterial ring method, can have influence on evaluation to fungistatic effect such as the diffusivity of antibacterial type fresh air compositions on flat board, this also is the persistence reason poorer slightly than streptomycin of the fungistatic effect of antibacterial type fresh air compositions in above-described embodiment 3, but the antibacterial type fresh air compositions of the present invention preparation all reaches more than 85% the sterilizing rate of escherichia coli and staphylococcus aureus.
Embodiment 4 antibacterial type fresh air preparation of compositions and the experiment of inhibition fungus effect thereof
1, preparation of compositions:
The 25ml cinnamon essential oil is added 75ml95% ethanol, stir, namely get the compositions A of 100ml antibacterial type fresh air.
The 10ml cinnamon essential oil is added 90ml95% ethanol, stir, namely get 100ml antibacterial type fresh air compositions B.
The 5ml cinnamon essential oil is added 95ml95% ethanol, stir, namely get 100ml antibacterial type fresh air compositions C.
Above-mentioned antibacterial type fresh air compositions need not pressurization, can be to the room air sterilization that carries out disinfection in the aerosol can of directly packing into, and simple to operate, simplified the production operation program greatly, applied widely.
Above-mentioned antibacterial type fresh air compositions, also available humidifier ultrasonic atomizatio is disseminated to the sterilization that carries out disinfection in the room air, and is simple to operate, simplified the production operation program greatly, applied widely.
2, the effect of antibacterial type fresh air compositions experiment
1) fungistatic effect of antibacterial around-France detection antibacterial type air compositions
Cut-off directly is the aseptic dry filter paper sheet of 5mm, and every drips the antibacterial type delicate fragrance air compositions that makes among the 20 μ L above-mentioned 1, simultaneously to drip with the sterilized water distilled water filter paper of volume in contrast.Above-mentioned filter paper is lain against respectively in the clean aseptic plate, uncap place dry under calorstat (37 ℃) oven dry or the room temperature afterwards standby.
Drawing concentration respectively with aseptic pipettor is 1x10
5Cfu/ml-9x10
5Each 100 μ L of the bacteria suspension of the Candida albicans of cfu/ml (Canidia Albicans) ATCC10231 evenly are coated with 3 times with spreading rod at the sabouraud's agar planar surface respectively.Whenever smear 1 time, flat board is rotated 60 °, smear a week with spreading rod along plate edge at last, build plate, put under the room temperature condition and cultivate, standby.
The dropping of getting above-mentioned preparation with aseptic nipper has the filter paper of antibacterial type fresh air compositions, sterilized water, paste respectively and be put on the above-mentioned flat board that is coated with different bacterium, each is dull and stereotyped to go up to paste and puts the filter paper that filter paper that 5 droppings have air freshener and 1 dropping have sterilized water.Between each filter paper center at a distance of more than the 25mm, and with the edge of flat board at a distance of more than the 15mm.After posting, with the light filter pressing scraps of paper of aseptic nipper, make it be close to planar surface.Build plate, place 28 ℃ of calorstats, observed result behind the cultivation 24h-48h.
With diameter and the Taking Pictures recording of the antibacterial ring of vernier caliper measurement, when measuring inhibition zone diameter, should select evenly and the antibacterial ring of integral asepsis growth carries out, be the boundary with antibacterial ring outer.Three repetitions are established in experiment, get the meansigma methods of three repetitions as experimental result.
Antibacterial circle diameter result of variations in time is as shown in table 7, d1 wherein, d2, d3, d4 represents the compositions A that drips the antibacterial type fresh air, the compositions B of antibacterial type fresh air, the antibacterial circle diameter measurement result of the compositions C of antibacterial type fresh air and sterile distilled water respectively.
Table 7 antibacterial circle diameter is result of variations in time
2) by adding up the effect of clump count detection antibacterial type fresh air compositions
Measure above-mentioned steps 1 respectively) the flat board that posts the filter paper that drips antibacterial type fresh air compositions on clump count, calculate sterilizing rate, the experiment triplicate, the result keeps integer, the result of sterilizing rate is as shown in table 8.
Sterilizing rate (%)=(I-II)/I * 100%
In the formula: I represents to drip the average clump count on the flat board of filter paper of sterilized water, and II drips the average clump count on the flat board of filter paper of antibacterial type fresh air compositions.
The fungicidal effect of table 8 antibacterial type fresh air compositions
From above experimental result as can be known, antibacterial type fresh air compositions of the present invention to the oidiomycetic killing rate of white more than 99%.
The above only is preferred embodiment of the present invention, and is in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, is equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. the compositions of an antibacterial type fresh air is characterized in that, in the percentage ratio of raw material cumulative volume, is made up of following raw material: solvent 70%-95%, and plants essential oil 5%-30%, wherein, described plants essential oil is cinnamon essential oil, described solvent is ethanol.
2. compositions according to claim 1 is characterized in that, described concentration of alcohol is 95%.
3. compositions according to claim 2 is characterized in that, is made up of following raw material: cinnamon essential oil 25ml, 95% ethanol 75ml.
4. compositions according to claim 2 is characterized in that, is made up of following raw material: cinnamon essential oil 10ml, 95% ethanol 90ml.
5. compositions according to claim 2 is characterized in that, is made up of following raw material: cinnamon essential oil 5ml, 95% alcohol 95 ml.
6. the preparation of compositions method of an antibacterial type fresh air is characterized in that, comprising:
1) adopt this area conventional method from plant, to extract cinnamon essential oil, or buy commercially available cinnamon essential oil product,
2) with cinnamon essential oil and etoh solvent mix homogeneously, namely.
As the arbitrary described compositions of claim 1 to 5 for the preparation of air freshener.
8. air freshener according to claim 7 is characterized in that, is used in inhibition air escherichia coli, staphylococcus aureus, the Candida albicans one or more.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110326627A (en) * | 2018-09-17 | 2019-10-15 | 中国医学科学院药用植物研究所 | Spray for refreshing air |
| CN111346023A (en) * | 2020-04-15 | 2020-06-30 | 广州立白企业集团有限公司 | Plant bacteriostatic composition, mouthwash containing composition and preparation method of mouthwash |
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| CN110326627B (en) * | 2018-09-17 | 2021-08-31 | 中国医学科学院药用植物研究所 | Air refreshing spray |
| CN111346023A (en) * | 2020-04-15 | 2020-06-30 | 广州立白企业集团有限公司 | Plant bacteriostatic composition, mouthwash containing composition and preparation method of mouthwash |
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