CN107365801A - 处理牛体细胞克隆胚的方法 - Google Patents
处理牛体细胞克隆胚的方法 Download PDFInfo
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- CN107365801A CN107365801A CN201710805551.9A CN201710805551A CN107365801A CN 107365801 A CN107365801 A CN 107365801A CN 201710805551 A CN201710805551 A CN 201710805551A CN 107365801 A CN107365801 A CN 107365801A
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- oxamflatin
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
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- C12N2500/00—Specific components of cell culture medium
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/065—Modulators of histone acetylation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
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Abstract
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201710805551.9A CN107365801B (zh) | 2017-09-08 | 2017-09-08 | 处理牛体细胞克隆胚的方法 |
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CN201710805551.9A CN107365801B (zh) | 2017-09-08 | 2017-09-08 | 处理牛体细胞克隆胚的方法 |
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CN107365801A true CN107365801A (zh) | 2017-11-21 |
CN107365801B CN107365801B (zh) | 2019-11-01 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112567016A (zh) * | 2018-08-20 | 2021-03-26 | 爱平世股份有限公司 | 细胞培养器 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102296090A (zh) * | 2011-08-29 | 2011-12-28 | 西北农林科技大学 | 一种基于体细胞核移植构建的牛体细胞克隆胚的处理方法 |
CN102648708A (zh) * | 2011-02-25 | 2012-08-29 | 深圳华大方舟生物技术有限公司 | 一种用于胚胎或细胞的冷冻液及其用途 |
CN103305551A (zh) * | 2013-06-09 | 2013-09-18 | 西北农林科技大学 | 一种基于囊胚基因表达水平筛选供体细胞系的方法 |
CN106417251A (zh) * | 2016-09-21 | 2017-02-22 | 中国水产科学研究院黄海水产研究所 | 一种高浓度玻璃化液及石斑鱼胚胎超低温冷冻保存方法 |
CN107460208A (zh) * | 2017-09-08 | 2017-12-12 | 英科博雅基因科技(天津)有限公司 | 哺乳动物体细胞克隆方法和使用的培养液 |
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2017
- 2017-09-08 CN CN201710805551.9A patent/CN107365801B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102648708A (zh) * | 2011-02-25 | 2012-08-29 | 深圳华大方舟生物技术有限公司 | 一种用于胚胎或细胞的冷冻液及其用途 |
CN102296090A (zh) * | 2011-08-29 | 2011-12-28 | 西北农林科技大学 | 一种基于体细胞核移植构建的牛体细胞克隆胚的处理方法 |
CN102296090B (zh) * | 2011-08-29 | 2013-04-24 | 西北农林科技大学 | 一种基于体细胞核移植构建的牛体细胞克隆胚的处理方法 |
CN103305551A (zh) * | 2013-06-09 | 2013-09-18 | 西北农林科技大学 | 一种基于囊胚基因表达水平筛选供体细胞系的方法 |
CN106417251A (zh) * | 2016-09-21 | 2017-02-22 | 中国水产科学研究院黄海水产研究所 | 一种高浓度玻璃化液及石斑鱼胚胎超低温冷冻保存方法 |
CN107460208A (zh) * | 2017-09-08 | 2017-12-12 | 英科博雅基因科技(天津)有限公司 | 哺乳动物体细胞克隆方法和使用的培养液 |
Non-Patent Citations (4)
Title |
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MAO ET AL: "Oxamflatin Treatment Enhances Cloned Porcine Embryo Development and Nuclear Reprogramming", 《CELLULAR REPROGRAMMING》 * |
TETSUO ONO ET AL: "Inhibition of Class Ilb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice", 《BIOLOGY OF REPRODUCTION》 * |
彭礼繁等: "不同蔗糖浓度、供体细胞保存温度和融合温度对小鼠体细胞克隆胚胎发育的影响", 《广东畜牧兽医科技》 * |
李晓霞等: "融合液浓度梯度融合法提高延边黄牛体细胞克隆效率", 《中国畜牧杂志》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112567016A (zh) * | 2018-08-20 | 2021-03-26 | 爱平世股份有限公司 | 细胞培养器 |
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Effective date of registration: 20180905 Address after: 300457 A building, Alexander center, 3 Haitong street, Tianjin Binhai New Area. Applicant after: Tianjin Bo Yu Li Husbandry Technology Co., Ltd. Applicant after: Tianjin Zhongke Boya biological Breeding Research Co Ltd Applicant after: Tianjin Li Mu Ding Feng Biotechnology Co., Ltd. Address before: 300457 Tianjin city Tanggu District Development Zone Haitong Street 3 Alexander center A floor, British Boya company Applicant before: Intco Boya (Tianjin) Co., Ltd. gene technology Applicant before: Tianjin Bo Yu Li Husbandry Technology Co., Ltd. Applicant before: Tianjin Zhongke Boya biological Breeding Research Co Ltd |
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Effective date of registration: 20190917 Address after: 300457 A building, Alexander center, 3 Haitong street, Tianjin Binhai New Area. Applicant after: Tianjin Bo Yu Li Husbandry Technology Co., Ltd. Applicant after: Tianjin Zhongke Boya biological Breeding Research Co Ltd Address before: 300457 A building, Alexander center, 3 Haitong street, Tianjin Binhai New Area. Applicant before: Tianjin Bo Yu Li Husbandry Technology Co., Ltd. Applicant before: Tianjin Zhongke Boya biological Breeding Research Co Ltd Applicant before: Tianjin Li Mu Ding Feng Biotechnology Co., Ltd. |
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Address after: 300457 building a, Alexandria, No.3 Haitong street, Binhai New Area Development Zone, Tianjin Boya company Patentee after: Tianjin Limu Biotechnology Co.,Ltd. Patentee after: Tianjin Boya biological breeding research Co., Ltd Address before: 300457 A building, Alexander center, 3 Haitong street, Tianjin Binhai New Area. Patentee before: TIANJIN BOYU LIMU TECHNOLOGY CO.,LTD. Patentee before: Tianjin Boya biological breeding research Co., Ltd |