CN107361082B - Application of regulating agent - Google Patents

Application of regulating agent Download PDF

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CN107361082B
CN107361082B CN201710663118.6A CN201710663118A CN107361082B CN 107361082 B CN107361082 B CN 107361082B CN 201710663118 A CN201710663118 A CN 201710663118A CN 107361082 B CN107361082 B CN 107361082B
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mixed solution
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following components
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CN107361082A (en
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郭汉华
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02—Amines; Quaternary ammonium compounds
    • A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the field of tobacco cultivation, and particularly relates to an application of a regulator, which at least comprises a component A, a component B and a component C, wherein the component A comprises the following components in parts by weight: 1-10 parts of polyamine, 1-10 parts of sodium nitroprusside and 2-5 parts of sodium sulfite; the component B comprises the following components in parts by weight: 0.5-3 parts of cobalt chloride, 50-150 parts of calcium chloride and 2-5 parts of mannitol; the component C comprises the following components in parts by weight: 10-20 parts of EDTA (ethylene diamine tetraacetic acid) and 20-30 parts of jasmonic acid; the component A, the component B and the component C are used separately. Experimental research shows that the phenomena of premature senility, whitening, burning and brown spots of field tobacco leaves are obviously reduced, the physiological and quality indexes are obviously improved, the using effect is obvious, the occurrence of the high-temperature induced maturity phenomenon of flue-cured tobacco can be effectively prevented and controlled, and the quality of the tobacco leaves is improved.

Description

Application of regulating agent
Technical Field
The invention relates to the field of tobacco cultivation, in particular to application of a regulating agent.
Background
Tobacco is a plant which is very sensitive to environmental condition change, ecological factors have great influence on the yield and quality of tobacco leaves, wherein climate factors are main ecological factors for producing high-quality characteristic tobacco leaves. Research shows that compared with foreign high-quality tobacco leaf production areas, the meteorological condition problem of the growing period of tobacco in the field of China mainly shows that: the sunshine is relatively insufficient; the early stage temperature is relatively low, and the later stage temperature is relatively high. This phenomenon is particularly prominent in the tobacco zone of the central and south China.
The south mountain range is an important climate boundary in China, south China, and has an obvious barrier effect on south China climate due to the special terrain constitution. In spring, due to the blocking effect of the mountains in the south mountains, cold air is retained in the north of the south mountains (southern Hunan tobacco area), and the rainy weather is more; in summer, when the subtropical zone controls the Yangtze river basin and the upper part of Hunan under high pressure, the windward side is prevailing, and in the north side of the south ridge, the downdraft is strong, so that the weather is clear, the temperature is high, the humidity is low, and the high-temperature weather is obvious. High-temperature and intense light radiation and other meteorological disasters which are unfavorable for the growth of the flue-cured tobacco easily appear in the tobacco area in the central and south China, so that the phenomenon of 'high-temperature maturity' occurs. In recent years, research on cultivation technology lags behind the rapid development of tobacco leaf production and the discordance of ecological factors, the phenomena of abnormal yellowing of tobacco leaves in the mature period, serious whitening, burning and brown spots of the tobacco leaves and the like frequently occur in the production of tobacco areas in the central south of China (such as southern Hunan tobacco area), and the quality and the usability of the tobacco leaves are influenced.
Because the phenomenon of 'high temperature maturity' has obvious regional characteristics, the understanding aspect is lack of enough attention for a long time, and the phenomena are extremely deficient in the aspects of theoretical research and prevention and control technology. Thus, in production practice, late high temperature hazards are currently avoided or reduced, mainly by early transplant periods. So far, no technical report for preventing and controlling high-temperature induced maturity of flue-cured tobacco by using a regulating agent is available.
In conclusion, a regulation and control agent which has a remarkable effect of preventing high-temperature induced ripening of flue-cured tobacco and can improve the quality of tobacco leaves is urgently needed.
Disclosure of Invention
The invention aims to develop a regulating agent which has a remarkable effect of preventing flue-cured tobacco from being matured at high temperature and can improve the quality of tobacco leaves and application thereof.
The above purpose is realized by the following technical scheme: the regulator is characterized by at least comprising a component A, a component B and a component C, wherein the component A comprises the following components in parts by weight: 1-10 parts of polyamine, 1-10 parts of sodium nitroprusside and 2-5 parts of sodium sulfite; the component B comprises the following components in parts by weight: 0.5-3 parts of cobalt chloride, 50-150 parts of calcium chloride and 2-5 parts of mannitol; the component C comprises the following components in parts by weight: 10-20 parts of EDTA (ethylene diamine tetraacetic acid) and 20-30 parts of jasmonic acid; the component A, the component B and the component C are used separately.
Researches show that under the action of protecting cells, air holes of tobacco at high temperature are closed to a certain extent to relieve the damage of high temperature and strong light to adapt to the high-temperature environment, but under the continuous or long-time high-temperature action, the tobacco leaves not only denature chloroplast proteins and cause abnormal respiration, but also accumulate a large amount of harmful substances such as active oxygen to damage a photosynthesis system, and simultaneously transpiration loses a large amount of water and causes abnormal physiological metabolism of leaves, so that the aging is accelerated, a plurality of yellow spots appear on leaf tissues before maturation, and the tobacco leaves are browned quickly, and the quality of the tobacco leaves is seriously influenced. Through research, the exogenous NO generated by decomposition of the sodium nitroprusside after being absorbed by the leaves has the function of inducing the closure of pores, meanwhile, the decomposition product of the sodium nitroprusside can increase the photosynthesis intensity, and the boundary product of the sodium nitroprusside can generate nitrosation reaction with active protein in the leaves, such as cysteine, so as to change the activity of the active protein; polyamine accessible restraines the ion channel and inhibits the gas pocket open, can induce the quick accumulation of blade NO simultaneously, guarantee that NO has the ability of induced gas pocket closing, and can strengthen its antioxidant capacity, thus, make full use of polyamine inhibits the function that the gas pocket is open and exogenous NO induced gas pocket is closed, fully restrain the respiration and the transpiration of blade, reduce harmful substance's accumulation and the loss of blade moisture, but the closing of gas pocket certain degree reduces respiration and transpiration simultaneously, can also restrain photosynthesis, experimental research shows, the spraying of sodium sulfite solution, greatly reduced the high temperature of tobacco plant and forced maturity effect to the influence of photosynthesis, concrete theory of action may be: sodium sulfite has certain reducibility, can react with active oxygen in leaves to reduce the content of active oxygen, and on the other hand, sulfite is reduced to H under the action of salt reductase in plants2S，H2S can react with active oxygen and peroxide to reduce the oxidative damage in cells, especially the damage of chlorophyll; and H2S can react with active protein in leaves to sulfhydrylate and activate oxidation resistanceThe enzyme activity strengthens the enzyme performance in an antioxidant system and an ascorbic acid-glutathione circulating system, and ensures the intensity of photosynthesis through the mutual coordination with the nitrosation of active protein.
On the other hand, research shows that the use of calcium chloride in the component B can reduce the accumulation of active oxygen in leaves, especially at high temperature, the treatment of calcium chloride in the regulator can enhance the scavenging capacity of active oxygen by maintaining or increasing the content of antioxidant activity and antioxidant substances (such as ascorbic acid) in tobacco leaves, reduce the damage of active oxygen to photosynthetic mechanisms, and simultaneously, Ca can reduce the damage of active oxygen to photosynthetic mechanisms2+The transmembrane transport reduces the electrolyte extravasation rate of the tobacco leaves and maintains the intracytoplasmic Ca2+Steady state balance, and research shows that calcium chloride can also improve the activity of rubisco activating enzyme in the leaves, so that higher photosynthetic rate of the tobacco leaves can be maintained even if the stomata of the leaves are induced to be closed; the experimental research shows that after the cobalt chloride solution is sprayed, the generation of ethylene can be effectively inhibited, the content of polyamine in leaves can be increased, the effective inhibition effect on the increase of the active oxygen level is achieved, the chlorophyll degradation is inhibited to a certain extent, and the activity of nitrate reductase can be effectively increased by adding mannitol, so that the catalysis of nitrate (nitrite) to form NO is facilitated. Therefore, the component B is sprayed before the component A is sprayed, which is beneficial to giving full play to the function of the component A, reducing the content or concentration of each component in the component A to a certain extent, saving the cost and simultaneously slowing down other normal physiological growth of the regulating agent to tobacco.
Thirdly, according to the invention, before A, B components are sprayed, EDTA and jasmonic acid in the C component are sprayed, and experimental studies show that: the leaves can activate the nitrate reductase to a certain extent after absorbing EDTA molecules, the inhibition effect of high temperature and strong light on the nitrate reductase is relieved, the NO can be favorably catalyzed to form nitrate (nitrite), meanwhile, the jasmonic acid shows a synergistic effect with the EDTA by adjusting the activity of the antioxidase, the plant cytoplasm concentration is increased by increasing the content of the permeation regulating substance, the osmotic potential is reduced to enhance the osmotic regulation capability and the water absorption capability of the tobacco leaves, a plant membrane system is protected, the influence caused by NO induced pore closure is relieved, and the normal metabolic level is maintained to a certain extent.
Experimental research shows that the phenomena of premature senility, whitening, burning and brown spots of field tobacco leaves are obviously reduced, physical and quality indexes are obviously improved (see the embodiment in detail), the use effect is obvious, the high-temperature induced maturity phenomenon of flue-cured tobacco can be effectively prevented and controlled, and the quality of the tobacco leaves is improved.
Preferably, the further technical scheme is as follows: the polyamine is one or more of putrescine, spermidine, spermine, putrescine hydrochloride, spermidine hydrochloride and spermine hydrochloride.
Preferably, the further technical scheme is as follows: the component A also comprises the following components in parts by weight: 100-300 parts of monopotassium phosphate. The monopotassium phosphate effectively supplements phosphorus and potassium elements, enhances the physiological metabolic activity and stress resistance of later-stage tobacco plants, promotes normal yellow-fall difference of leaves, simultaneously has the effects of reducing alkali and increasing potassium, inhibits nicotine synthesis, promotes potassium absorption and accumulation, promotes top leaf opening and improves the chemical component harmony of tobacco leaves.
Preferably, the further technical scheme is as follows: the component C also comprises the following components in parts by weight: 10-20 parts of salicylic acid. Experiments show that the salicylic acid can stimulate tobacco plants to synthesize a large amount of heat shock proteins by spraying, keep the activity of higher superoxide dismutase and catalase in the body, reduce the accumulation of harmful substances and active oxygen in leaves, improve the anti-oxidation stress capability of the plants, reduce the damage of oxidation stress caused by high temperature to the plants, and maintain the stability of cell membranes, thereby improving the heat resistance.
Preferably, the further technical scheme is as follows: the component A comprises the following components in parts by weight: 5 parts of putrescine hydrochloride, 5 parts of sodium nitroprusside, 200 parts of monopotassium phosphate and 3 parts of sodium sulfite; the component B comprises the following components in parts by weight: 2 parts of cobalt chloride, 100 parts of calcium chloride and 3 parts of mannitol; the component C comprises the following components in parts by weight: 15 parts of EDTA, 25 parts of jasmonic acid and 15 parts of salicylic acid. Therefore, through proper blending, the proportion of each component of the regulating agent is determined, the synergistic effect of each component is fully exerted, the respiration and transpiration of tobacco leaves are inhibited as far as possible under a high-temperature environment, the photosynthetic rate of the tobacco leaves is maintained, the accumulation of harmful substances and active oxygen in the leaves is reduced, the scavenging capacity of the active oxygen in the leaves is improved, the physiological metabolic activity and stress resistance of the tobacco plants are enhanced, and the physiological and quality indexes of tobacco leaves are improved. Experimental research shows that after the regulating agent with the components in the proportion is sprayed, the high-temperature induced ripening effect of tobacco plants is obviously weakened, and the phenomena of premature senility, whitening, burning, brown spots and the like of tobacco leaves are obviously improved.
Preferably, the further technical scheme is as follows: the regulator also comprises a component D, wherein the component D comprises the following components in parts by weight: 10-20 parts of sodium bicarbonate. When the spraying time of the component D is within the interval time of spraying the component A for multiple times, researches show that the spraying time of the component D is favorable for reducing the contents of protein, nicotine and total nitrogen in the tobacco leaves, improving the contents of reducing sugar, total sugar and potassium, improving the harmony of chemical components and improving the quality of the tobacco leaves.
In another aspect, the present invention also provides the use of any of the modulators described above: respectively dissolving the component A, the component B and the component C of the regulating agent in water, and sequentially spraying a component C mixed solution, a component B mixed solution and a component A mixed solution on leaf surfaces of tobacco plants from a vigorous growing period to a mature period, wherein the spraying time interval of the component C mixed solution, the component B mixed solution and the component A mixed solution is 2-5 days, the component A mixed solution is sprayed for 1-3 times, and the spraying time interval of the component A mixed solution is 8-15 days.
According to the invention, the C component mixed solution, the B component mixed solution and the A component mixed solution are sequentially sprayed on the leaf surface, as mentioned above, the nitrate reductase can be activated to a certain extent after the EDTA of the C component and the mannitol of the B component are absorbed by the leaves, the activity of the nitrate reductase can be effectively increased, the nitric acid reductase can be fully desorbed and released NO, and the action effect of the nitric acid reductase is improved. And after the jasmonic acid and the salicylic acid in the component C and the cobalt chloride and the calcium chloride in the component B are sprayed and absorbed sufficiently by the leaves, the effects of preventing high-temperature maturity and regulating the normal growth of plants are achieved, each system of plant individuals is protected, the influence caused by NO-induced pore closure is relieved, the normal metabolic level of the plants is maintained, and the chemical component coordination of the tobacco leaves is improved.
Experimental research shows that after the diluent of the regulating agent is sprayed in the manner, the high-temperature induced maturity phenomenon of flue-cured tobacco is effectively prevented and controlled, the phenomena of premature senility, whitening, burning and brown spots of field tobacco leaves are obviously reduced, the physiological and quality indexes are obviously improved, the using effect is obvious, and the quality of the tobacco leaves can be effectively improved.
Preferably, the further technical scheme is as follows: the dosage of the mixed solution of the component C is 40-80 kg per mu, the dosage of the mixed solution of the component B is 30-60 kg per mu, the dosage of the mixed solution of the component A is 30-100 kg per mu, the mass ratio of the total mass of the component B to water in the mixed solution of the component C is 1: 200-500, the mass ratio of the total mass of the component B to water in the mixed solution of the component B is 1: 300-800, and the mass ratio of the total mass of the component A to water in the mixed solution of the component A is 1: 200-1000.
Preferably, the further technical scheme is as follows: spraying the mixed solution of the component C on the leaf surfaces of the tobacco plants in the vigorous growth middle period, wherein the dosage of each mixed solution is 50kg per mu, spraying the mixed solution of the component B after 3 days, the dosage of each mixed solution is 60kg per mu, spraying the mixed solution of the component A for the first time after 3 days, and spraying the mixed solution of the component A for the second time after 10 days, wherein the dosage of each mixed solution is 70kg per mu, the mass ratio of the total mass of the component C to the water in the mixed solution of the component C is 1:400, the mass ratio of the total mass of the component B to the water in the mixed solution of the component B is 1:600, and the mass ratio of the total mass of the component A to the water in the mixed solution of the component A is 1: 800.
Preferably, the further technical scheme is as follows: the regulator also comprises a component D, wherein the component D comprises the following components in parts by weight: 10-20 parts of sodium bicarbonate; dissolving the component D in water to obtain a mixed solution of the component D, spraying the mixed solution of the component D at intervals of 3-7 days after spraying the mixed solution of the component A for the first time, wherein the using amount of the mixed solution of the component D is 50-100 kg per mu, and the mass ratio of the total mass of the component D to the water in the mixed solution of the component D is 1: 100-300.
When the spraying time of the component D is within the interval time of spraying the component A for multiple times, researches show that the spraying time of the component D is favorable for reducing the contents of protein, nicotine and total nitrogen in the tobacco leaves, improving the contents of reducing sugar, total sugar and potassium, improving the harmony of chemical components and improving the quality of the tobacco leaves.
Detailed Description
The invention will be further described with reference to specific examples to assist a better understanding of the invention, but the scope of the invention is not limited to these examples.
Example 1
Uniformly mixing 15 parts of EDTA (ethylene diamine tetraacetic acid) and 25 parts of jasmonic acid in the component C by weight, dissolving the mixture in water to obtain a component C mixed solution, and spraying the component C mixed solution on the leaf surfaces of the tobacco plants in the vigorous growing medium stage, wherein the dosage of each time is 50kg per mu; spraying a mixed solution of a component B after 3 days, wherein the dosage of each time is 50kg per mu, and the mixed solution of the component B is a mixed solution obtained by uniformly mixing 2 parts by weight of cobalt chloride, 100 parts by weight of calcium chloride and 3 parts by weight of mannitol and dissolving the mixture in water; spraying the mixed solution of the component A for the first time after 3 days, and spraying the mixed solution of the component A for the second time after 10 days, wherein the dosage of each time is 70kg per mu, and the mixed solution of the component A is a mixed solution obtained by uniformly mixing 5 parts by weight of putrescine hydrochloride, 5 parts by weight of sodium nitroprusside and 3 parts by weight of sodium sulfite and dissolving the mixture in water; wherein the mass ratio of the total mass of the component C to the water in the mixed solution of the component C is 1:400, the mass ratio of the total mass of the component B to the water in the mixed solution of the component B is 1:600, and the mass ratio of the total mass of the component A to the water in the mixed solution of the component A is 1: 800.
Example 2
Uniformly mixing 10 parts of EDTA and 30 parts of jasmonic acid in the component C by weight, dissolving the mixture in water to obtain a component C mixed solution, and spraying the component C mixed solution on the leaf surfaces of the tobacco plants in the middle and later stages of vigorous growth, wherein the dosage of each time is 40kg per mu; spraying a mixed solution of a component B after 2 days, wherein the dosage of each time is 60kg per mu, and the mixed solution of the component B is a mixed solution obtained by uniformly mixing 3 parts by weight of cobalt chloride, 50 parts by weight of calcium chloride and 5 parts by weight of mannitol and dissolving the mixture in water; spraying the mixed solution of the component A for the first time after 5 days, and spraying the mixed solution of the component A for the second time after 8 days, wherein the dosage of each time is 100kg per mu, and the mixed solution of the component A is a mixed solution obtained by uniformly mixing 1 part by weight of putrescine, 10 parts by weight of sodium nitroprusside and 5 parts by weight of sodium sulfite and dissolving the mixture in water; wherein the mass ratio of the total mass of the component C to the water in the mixed solution of the component C is 1:500, the mass ratio of the total mass of the component B to the water in the mixed solution of the component B is 1:300, and the mass ratio of the total mass of the component A to the water in the mixed solution of the component A is 1: 1000.
Example 3
Uniformly mixing 20 parts of EDTA (ethylene diamine tetraacetic acid) and 20 parts of jasmonic acid in the component C by weight, dissolving the mixture in water to obtain a component C mixed solution, and spraying the component C mixed solution on the leaf surfaces of the tobacco plants in the vigorous growing medium stage, wherein the dosage of each time is 80kg per mu; spraying a mixed solution of a component B after 5 days, wherein the dosage of each time is 30kg per mu, and the mixed solution of the component B is a mixed solution obtained by uniformly mixing 0.5 part by weight of cobalt chloride, 150 parts by weight of calcium chloride and 2 parts by weight of mannitol and then dissolving the mixture in water; spraying the mixed solution of the component A for the first time after 2 days, and spraying the mixed solution of the component A for the second time after 15 days, wherein the using amount of each time is 30kg per mu, and the mixed solution of the component A is a mixed solution obtained by uniformly mixing 10 parts by weight of putrescine, 1 part by weight of sodium nitroprusside and 2 parts by weight of sodium sulfite and dissolving the mixture in water; wherein the mass ratio of the total mass of the component C to the water in the mixed solution of the component C is 1:200, the mass ratio of the total mass of the component B to the water in the mixed solution of the component B is 1:800, and the mass ratio of the total mass of the component A to the water in the mixed solution of the component A is 1: 200.
Example 4
On the basis of example 2, the component a mixed solution was sprayed a third time at an interval of 8 days after the component a mixed solution was sprayed a second time.
Example 5
On the basis of the example 1, the component A also comprises 200 parts of monopotassium phosphate.
The research shows that: the potassium dihydrogen phosphate can achieve a good effect when the mass content is 100-300 parts.
Example 6
On the basis of the embodiment 1, the component C also comprises 15 parts of salicylic acid.
The research shows that: the salicylic acid content can reach better effect when 10-20 parts.
Example 7
On the basis of example 5, the component C also comprises 15 parts of salicylic acid.
The research shows that: the salicylic acid content can reach better effect when 10-20 parts.
Example 8
On the basis of example 7, the mixed solution of the component D is sprayed at an interval of 5 days after the mixed solution of the component a is sprayed for the first time, wherein the component D comprises 15 parts by weight of sodium bicarbonate, the mixed solution of the component D is obtained by dissolving the component D in water, the amount of the mixed solution of the component D is 70kg per mu, and the mass ratio of the total mass of the component D to the water in the mixed solution of the component D is 1: 200.
Example 9
On the basis of the embodiment 2, the mixed solution of the component D is sprayed at intervals of 3 days after the mixed solution of the component A is sprayed for the first time, wherein the component D comprises 20 parts by weight of sodium bicarbonate; and (3) dissolving the component D in water to obtain a mixed solution of the component D, wherein the using amount of the mixed solution of the component D is 50kg per mu, and the mass ratio of the total mass of the component D to the water in the mixed solution of the component D is 1: 100.
Example 10
On the basis of the embodiment 3, the mixed solution of the component D is sprayed at an interval of 7 days after the mixed solution of the component A is sprayed for the first time, wherein the component D comprises 10 parts by weight of sodium bicarbonate; and (3) dissolving the component D in water to obtain a mixed solution of the component D, wherein the using amount of the mixed solution of the component D is 100kg per mu, and the mass ratio of the total mass of the component D to the water in the mixed solution of the component D is 1: 300.
On the basis of the embodiments 1 and 4-6, the component D solution can be sprayed in the spraying interval of the component A mixed solution, and researches show that better effects can be achieved.
Experimental results show that under the above embodiment, the high-temperature induced ripening phenomenon of the flue-cured tobacco is effectively inhibited, the phenomena of premature senility, whitening, burning and brown spots of the field tobacco are obviously reduced, the physiological and quality indexes are obviously improved, the quality of the tobacco is obviously improved, and the effect is obvious.
Comparative example
The conditions were the same as in example 1 except that water was sprayed without the addition of a conditioning agent.
As shown in tables 1-3, the physiological and quality indexes of the tobacco leaves sprayed with the regulating agent are obviously improved, the using effect is obviously improved, the high-temperature induced maturity phenomenon of the flue-cured tobacco can be effectively prevented and controlled, and the quality of the tobacco leaves is obviously improved.
TABLE 1 Effect of the Conditioning agent on the pigment content of tobacco leaves (maturity determination)
Figure GDA0002187016830000101
TABLE 2 Effect of the regulators on photosynthesis of tobacco leaves (maturity determination)
Figure GDA0002187016830000111
TABLE 3 Effect of the Conditioning Agents on the chemical composition of flue-cured tobacco leaves
Figure GDA0002187016830000112
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (9)

1. The application of the regulator is characterized by at least comprising a component A, a component B and a component C, wherein the component A comprises the following components in parts by weight: 1-10 parts of polyamine, 1-10 parts of sodium nitroprusside and 2-5 parts of sodium sulfite; the component B comprises the following components in parts by weight: 0.5-3 parts of cobalt chloride, 50-150 parts of calcium chloride and 2-5 parts of mannitol; the component C comprises the following components in parts by weight: 10-20 parts of EDTA (ethylene diamine tetraacetic acid) and 20-30 parts of jasmonic acid; the component A, the component B and the component C are used separately; respectively dissolving the component A, the component B and the component C of the regulating agent in water, and sequentially spraying a component C mixed solution, a component B mixed solution and a component A mixed solution on leaf surfaces of tobacco plants from a vigorous growing period to a mature period, wherein the spraying time interval of the component C mixed solution, the component B mixed solution and the component A mixed solution is 2-5 days, the component A mixed solution is sprayed for 1-3 times, and the spraying time interval of the component A mixed solution is 8-15 days.
2. The use of the modulator according to claim 1, wherein the polyamine is one or more of putrescine, spermidine, spermine, putrescine hydrochloride, spermidine hydrochloride, and spermine hydrochloride.
3. The use of the modulator according to claim 2, wherein the component a further comprises the following components in parts by weight: 100-300 parts of monopotassium phosphate.
4. The use of the modulator according to claim 3, wherein the C component further comprises the following components in parts by weight: 10-20 parts of salicylic acid.
5. The use of the modulator according to claim 4, wherein the component A comprises the following components in parts by weight: 5 parts of putrescine hydrochloride, 5 parts of sodium nitroprusside, 200 parts of monopotassium phosphate and 3 parts of sodium sulfite; the component B comprises the following components in parts by weight: 2 parts of cobalt chloride, 100 parts of calcium chloride and 3 parts of mannitol; the component C comprises the following components in parts by weight: 15 parts of EDTA, 25 parts of jasmonic acid and 15 parts of salicylic acid.
6. The use of the regulating agent according to any one of claims 1 to 5, wherein the regulating agent further comprises a component D, and the component D comprises the following components in parts by weight: 10-20 parts of sodium bicarbonate.
7. The application of the regulator as claimed in claim 6, wherein the dosage of the mixed solution of the component C is 40-80 kg per acre, the dosage of the mixed solution of the component B is 30-60 kg per acre, the dosage of the mixed solution of the component A is 30-100 kg per acre, the mass ratio of the total mass of the component C to water in the mixed solution of the component C is 1: 200-500, the mass ratio of the total mass of the component B to water in the mixed solution of the component B is 1: 300-800, and the mass ratio of the total mass of the component A to water in the mixed solution of the component A is 1: 200-1000.
8. The application of the regulating agent as claimed in claim 7, wherein the mixed solution of the component C is sprayed on the leaf surfaces of the tobacco plants in the vigorous growth and middle stages, the dosage is 50kg per mu each time, the mixed solution of the component B is sprayed after 3 days, the mixed solution of the component A is sprayed for the first time after 3 days, the mixed solution of the component A is sprayed for the second time after 10 days, the mixed solution of the component A is sprayed for the second time, the dosage is 70kg per mu each time, the mass ratio of the total mass of the component C to the mass of the water in the mixed solution of the component C is 1:400, the mass ratio of the total mass of the component B to the mass of the water in the mixed solution of the component B is 1:600, and the mass ratio of the total mass of the component A to the mass of the water in the mixed solution of the component A is 1: 800.
9. The use of the modulator of claim 8, wherein the modulator further comprises a D-component comprising, in parts by weight: 10-20 parts of sodium bicarbonate; dissolving the component D in water to obtain a mixed solution of the component D, spraying the mixed solution of the component D at intervals of 3-7 days after spraying the mixed solution of the component A for the first time, wherein the using amount of the mixed solution of the component D is 50-100 kg per mu, and the mass ratio of the total mass of the component D to the water in the mixed solution of the component D is 1: 100-300.
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