CN107353151A - The preparation method of microbial manure - Google Patents
The preparation method of microbial manure Download PDFInfo
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- CN107353151A CN107353151A CN201710534410.8A CN201710534410A CN107353151A CN 107353151 A CN107353151 A CN 107353151A CN 201710534410 A CN201710534410 A CN 201710534410A CN 107353151 A CN107353151 A CN 107353151A
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- tunning
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- microbial manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Mechanical Engineering (AREA)
- Environmental & Geological Engineering (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Fertilizers (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention discloses a kind of preparation method of microbial manure, including:Rape cake is pre-processed, obtains the first tunning;Pretreated straw, obtain the second tunning;Take garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, leek and konjaku complete stool, crush, be well mixed, add water to cook 7~8 hours, photosynthetic bacteria is accessed into filter residue and carries out anaerobic fermentation, obtains the 3rd tunning;Potassium solubilizing bacteria, phosphate solubilizing bacteria, bacillus subtilis, photosynthetic bacteria are taken, mixes, obtains mix bacterium agent;First tunning, the second tunning, the 3rd tunning and mix bacterium agent are mixed, the filtrate that mixture is obtained with step 3 is soaked 2~3 days, syrup is then sprayed and is fermented, treats that central temperature stops fermenting higher than 50 DEG C, air-dries tunning, produce.The present invention can significantly reduce the usage amount of chemical fertilizer, and can loose soil property, effectively suppress pest and disease damage, increase unit area crop yield.
Description
Technical field
The present invention relates to bio-fertilizer field.A kind of it is more particularly related to micro- life comprising rape cake and stalk
The preparation method of thing fertilizer.
Background technology
Fertilising can improve crop yield per unit area, and China can be supported with to account for the arable land in the world 7% and account for the world 22%
Population, chemical fertilizer play the important and pivotal role.But the excess of chemical fertilizer and unreasonable use bring soil hardening, soil
The problems such as fertility decline, environmental pollution, ecological disruption.Therefore, a kind of new-type fertilizer for overcoming above mentioned problem of design is needed badly,
Reduce the usage amount of chemical fertilizer.
The content of the invention
It is an object of the present invention to provide a kind of preparation method of microbial manure, and it can significantly reduce making for chemical fertilizer
Dosage, and can loose soil property, effectively suppress pest and disease damage, increase unit area crop yield.
In order to realize according to object of the present invention and further advantage, there is provided a kind of preparation side of microbial manure
Method, including:
Boiled 2~3 hours Step 1: rape cake is put into boiling water, fermentation of Aspergillus niger is inoculated with after taking-up 5~7 days, Ran Houjie
Kind photosynthetic bacteria carries out anaerobic fermentation 7~10 days, obtains the first tunning;
Step 2: take stalk, crush, soaked 40~48 hours with ammonia spirit, put into boiling water that to boil 2~3 small after taking-up
When, then soaked with hydrogen peroxide solution, cellulose-decomposing bacterium is inoculated with after taking-up and is fermented 7~10 days, is then inoculated with lichens brood cell's bar
Bacterium and thermophilic actinomycete carry out aerobic fermentation 15~20 days, obtain the second tunning;
Step 3: take garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese toon
Leaf, leek and konjaku complete stool, crush, be well mixed, add water to cook 7~8 hours, separating filtrate and filter residue, connect into filter residue
Enter photosynthetic bacteria and carry out anaerobic fermentation, taken out after 5~7 days, dry, obtain the 3rd tunning;
Step 4: taking potassium solubilizing bacteria, phosphate solubilizing bacteria, bacillus subtilis, photosynthetic bacteria, mix, obtain mix bacterium agent;
Step 5: with 100:20~30:10~15:2~3 weight ratio is by the first tunning, the second tunning,
Three tunnings and mix bacterium agent mixing, the filtrate that mixture is obtained with step 3 is soaked 2~3 days, then spray syrup and enter
Row fermentation, treat that central temperature stops fermenting higher than 50 DEG C, air-dry tunning, obtain microbial manure.
Preferably, the preparation method of described microbial manure, the specific method bag of stalk is soaked with hydrogen peroxide solution
Include:
The stalk taken out from boiling water is dried, then put into reactor, hydrogen peroxide solution is added, then passes to ozone
Pressure is 500KPa in reactor, and is kept for 10~15 minutes, and then the pressure in reactor is reduced to 50kPa, and
Kept for 10~15 minutes, repeat above-mentioned to be passed through ozone pressurization and reduced pressure operation 3~5 times.
Preferably, the preparation method of described microbial manure, in step 3, garlic, purslane, cordate houttuynia, mulberry leaf,
Lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, leek and the weight of konjaku complete stool ratio are:1:2~3:0.5~
1:1~2:1~2:1~2:1~2:2~3:2~3:0.5~1:0.5~1:3~4.
Preferably, the preparation method of described microbial manure, in step 2, the mass fraction of ammonia spirit is
30%.
Preferably, the preparation method of described microbial manure, in step 2, the mass fraction of hydrogen peroxide solution is
20%.
Preferably, the preparation method of described microbial manure, viable count is 1 × 10 in mix bacterium agent9cfu/g。
Preferably, the preparation method of described microbial manure, stalk is crushed to 50~80 mesh.
The present invention comprises at least following beneficial effect:
1st, primary raw material of the invention is stalk and rape cake, belongs to twice laid, and abundance, thus is worth pushing away
Extensively.
2nd, the present invention can loose soil property, reduce soil hardening, using mix bacterium agent improve soil physical chemistry state, be soil
Lasting fertility is provided, and can substantially improve crop yield.
3rd, the 3rd tunning of the invention can cover the fragrance of rape cake, reduce attraction of the rape cake to insect, the
Three tunnings can also reduce the generation of pest and disease damage, for example reduce the incidence of fusarium root rot of maize.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
Embodiment 1
A kind of preparation method of microbial manure, including:
Boiled 2 hours Step 1: rape cake is put into boiling water, fermentation of Aspergillus niger is inoculated with after taking-up 5 days, be then inoculated with photosynthetic
Bacterium carries out anaerobic fermentation 7 days, obtains the first tunning;
Step 2: take stalk, crush, soaked 40 hours with ammonia spirit, put into after taking-up in boiling water and boil 2 hours, then
Soaked with hydrogen peroxide solution, cellulose-decomposing bacterium is inoculated with after taking-up and is fermented 7~10 days, is then inoculated with bacillus licheniformis and high temperature
Actinomyces carry out aerobic fermentation 15 days, obtain the second tunning;
Step 3: take garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese toon
Leaf, leek and konjaku complete stool, crush, be well mixed, add water to cook 7 hours, separating filtrate and filter residue, light is accessed into filter residue
Close bacterium and carry out anaerobic fermentation, taken out after 5 days, dry, obtain the 3rd tunning;
Step 4: taking potassium solubilizing bacteria, phosphate solubilizing bacteria, bacillus subtilis, photosynthetic bacteria, mix, obtain mix bacterium agent;
Step 5: with 100:20:10:2 weight ratio is by the first tunning, the second tunning, the 3rd tunning
Mixed with mix bacterium agent, the filtrate that mixture is obtained with step 3 is soaked 2 days, then sprayed syrup and fermented, treat center
Temperature stops fermenting higher than 50 DEG C, air-dries tunning, obtains microbial manure.
The preparation method of described microbial manure, the specific method of stalk is soaked with hydrogen peroxide solution to be included:
The stalk taken out from boiling water is dried, then put into reactor, hydrogen peroxide solution is added, then passes to ozone
Pressure is 500KPa in reactor, and is kept for 10 minutes, and then the pressure in reactor is reduced to 50kPa, and keeps
10 minutes, repeat above-mentioned to be passed through ozone pressurization and reduced pressure operation 3 times.
The preparation method of described microbial manure, in step 3, garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsam pear
Rattan, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, the weight ratio of leek and konjaku complete stool are:1:2:0.5:1:1:1:1:2:2:
0.5:0.5:3。
The preparation method of described microbial manure, in step 2, the mass fraction of ammonia spirit is 30%.
The preparation method of described microbial manure, in step 2, the mass fraction of hydrogen peroxide solution is 20%.
The preparation method of described microbial manure, viable count is 1 × 10 in mix bacterium agent9cfu/g。
The preparation method of described microbial manure, stalk is crushed to 50 mesh.
Embodiment 2
A kind of preparation method of microbial manure, including:
Boiled 3 hours Step 1: rape cake is put into boiling water, fermentation of Aspergillus niger is inoculated with after taking-up 7 days, be then inoculated with photosynthetic
Bacterium carries out anaerobic fermentation 10 days, obtains the first tunning;
Step 2: take stalk, crush, soaked 48 hours with ammonia spirit, put into after taking-up in boiling water and boil 3 hours, then
Soaked with hydrogen peroxide solution, cellulose-decomposing bacterium is inoculated with after taking-up and is fermented 10 days, then bacillus licheniformis is inoculated with and high temperature is put
Line bacterium carries out aerobic fermentation 20 days, obtains the second tunning;
Step 3: take garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese toon
Leaf, leek and konjaku complete stool, crush, be well mixed, add water to cook 8 hours, separating filtrate and filter residue, light is accessed into filter residue
Close bacterium and carry out anaerobic fermentation, taken out after 7 days, dry, obtain the 3rd tunning;
Step 4: taking potassium solubilizing bacteria, phosphate solubilizing bacteria, bacillus subtilis, photosynthetic bacteria, mix, obtain mix bacterium agent;
Step 5: with 100:30:15:3 weight ratio is by the first tunning, the second tunning, the 3rd tunning
Mixed with mix bacterium agent, the filtrate that mixture is obtained with step 3 is soaked 3 days, then sprayed syrup and fermented, treat center
Temperature stops fermenting higher than 50 DEG C, air-dries tunning, obtains microbial manure.
The preparation method of described microbial manure, the specific method of stalk is soaked with hydrogen peroxide solution to be included:
The stalk taken out from boiling water is dried, then put into reactor, hydrogen peroxide solution is added, then passes to ozone
Pressure is 500KPa in reactor, and is kept for 15 minutes, and then the pressure in reactor is reduced to 50kPa, and keeps
15 minutes, repeat above-mentioned to be passed through ozone pressurization and reduced pressure operation 5 times.
The preparation method of described microbial manure, in step 3, garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsam pear
Rattan, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, the weight ratio of leek and konjaku complete stool are:1:3:1:2:2:2:2:3:3:1:
1:4。
The preparation method of described microbial manure, in step 2, the mass fraction of ammonia spirit is 30%.
The preparation method of described microbial manure, in step 2, the mass fraction of hydrogen peroxide solution is 20%.
The preparation method of described microbial manure, viable count is 1 × 10 in mix bacterium agent9cfu/g。
The preparation method of described microbial manure, stalk is crushed to 80 mesh.
Embodiment 3
A kind of preparation method of microbial manure, including:
Boiled 3 hours Step 1: rape cake is put into boiling water, fermentation of Aspergillus niger is inoculated with after taking-up 6 days, be then inoculated with photosynthetic
Bacterium carries out anaerobic fermentation 9 days, obtains the first tunning;
Step 2: take stalk, crush, soaked 42 hours with ammonia spirit, put into after taking-up in boiling water and boil 3 hours, then
Soaked with hydrogen peroxide solution, cellulose-decomposing bacterium is inoculated with after taking-up and is fermented 9 days, is then inoculated with bacillus licheniformis and high temperature unwrapping wire
Bacterium carries out aerobic fermentation 18 days, obtains the second tunning;
Step 3: take garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese toon
Leaf, leek and konjaku complete stool, crush, be well mixed, add water to cook 8 hours, separating filtrate and filter residue, light is accessed into filter residue
Close bacterium and carry out anaerobic fermentation, taken out after 6 days, dry, obtain the 3rd tunning;
Step 4: taking potassium solubilizing bacteria, phosphate solubilizing bacteria, bacillus subtilis, photosynthetic bacteria, mix, obtain mix bacterium agent;
Step 5: with 100:25:13:3 weight ratio is by the first tunning, the second tunning, the 3rd tunning
Mixed with mix bacterium agent, the filtrate that mixture is obtained with step 3 is soaked 3 days, then sprayed syrup and fermented, treat center
Temperature stops fermenting higher than 50 DEG C, air-dries tunning, obtains microbial manure.
The preparation method of described microbial manure, the specific method of stalk is soaked with hydrogen peroxide solution to be included:
The stalk taken out from boiling water is dried, then put into reactor, hydrogen peroxide solution is added, then passes to ozone
Pressure is 500KPa in reactor, and is kept for 12 minutes, and then the pressure in reactor is reduced to 50kPa, and keeps
12 minutes, repeat above-mentioned to be passed through ozone pressurization and reduced pressure operation 4 times.
The preparation method of described microbial manure, in step 3, garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsam pear
Rattan, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, the weight ratio of leek and konjaku complete stool are:1:3:0.5:1:2:1:1:2:3:
1:1:4。
The preparation method of described microbial manure, in step 2, the mass fraction of ammonia spirit is 30%.
The preparation method of described microbial manure, in step 2, the mass fraction of hydrogen peroxide solution is 20%.
The preparation method of described microbial manure, viable count is 1 × 10 in mix bacterium agent9cfu/g。
The preparation method of described microbial manure, stalk is crushed to 60 mesh.
Comparative example 1
Do not prepare and using the 3rd tunning, for remaining parameter with identical in embodiment 3, technical process is also complete
It is identical.
Comparative example 2
In step 2, after stalk is crushed directly inoculation cellulose-decomposing bacterium fermentation, remaining parameter with it is complete in embodiment 3
Exactly the same, technical process is also identical.
Experiment
12 mu of the maize planting in condition identical field, the fertilizer of 3 mu of application embodiments 3 is chosen, choose 3 mu of applications pair
The fertilizer of ratio 1, the fertilizer of 3 mu of application comparative examples 2 is chosen, choose 3 mu and apply corn routine composite fertilizer (check experiment).Statistics
The incidence of fusarium root rot of maize, corn yield (average value) is counted after harvesting, the results are shown in Table 1.
Table 1
Corn yield (kg) | Root rot incidence (%) | |
Embodiment 3 | 831.5 | 0.41 |
Comparative example 1 | 790.9 | 5.49 |
Comparative example 2 | 793.6 | 0.81 |
Check experiment | 710.5 | 7.57 |
As shown in Table 1, the corn yield of embodiment 3 is apparently higher than comparative example 1, comparative example 2 and check experiment, root rot
Incidence is also significantly lower than comparative example 1, comparative example 2 and check experiment.Therefore, microbial manure of the invention can improve corn
Yield, and reduce the incidence of fusarium root rot of maize.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the embodiment with description.
Claims (7)
- A kind of 1. preparation method of microbial manure, it is characterised in that including:Boiled 2~3 hours Step 1: rape cake is put into boiling water, fermentation of Aspergillus niger is inoculated with after taking-up 5~7 days, be then inoculated with light Close bacterium and carry out anaerobic fermentation 7~10 days, obtain the first tunning;Step 2: take stalk, crush, soaked 40~48 hours with ammonia spirit, put into after taking-up in boiling water and boil 2~3 hours, so Soaked afterwards with hydrogen peroxide solution, cellulose-decomposing bacterium is inoculated with after taking-up and is fermented 7~10 days, is then inoculated with bacillus licheniformis and height Warm actinomyces carry out aerobic fermentation 15~20 days, obtain the second tunning;Step 3: take garlic, purslane, cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, fragrant-flowered garlic Dish and konjaku complete stool, crush, be well mixed, add water to cook 7~8 hours, separating filtrate and filter residue, accessed into filter residue photosynthetic Bacterium carries out anaerobic fermentation, is taken out after 5~7 days, dries, obtains the 3rd tunning;Step 4: taking potassium solubilizing bacteria, phosphate solubilizing bacteria, bacillus subtilis, photosynthetic bacteria, mix, obtain mix bacterium agent;Step 5: with 100:20~30:10~15:2~3 weight ratio sends out the first tunning, the second tunning, the 3rd Ferment product and mix bacterium agent mixing, the filtrate that mixture is obtained with step 3 is soaked 2~3 days, then spray syrup and sent out Ferment, treat that central temperature stops fermenting higher than 50 DEG C, air-dry tunning, obtain microbial manure.
- 2. the preparation method of microbial manure as claimed in claim 1, it is characterised in that soak stalk with hydrogen peroxide solution Specific method includes:The stalk taken out from boiling water is dried, then put into reactor, adds hydrogen peroxide solution, then passes to ozone to anti- It is 500KPa to answer pressure in kettle, and is kept for 10~15 minutes, and then the pressure in reactor is reduced to 50kPa, and keeps 10~15 minutes, repeat above-mentioned to be passed through ozone pressurization and reduced pressure operation 3~5 times.
- 3. the preparation method of microbial manure as claimed in claim 1, it is characterised in that in step 3, garlic, purslane, Cordate houttuynia, mulberry leaf, lotus leaf, balsampear stem, pine needle, argy wormwood, oenanthe stolonifera, Chinese Toon Leaves, the weight ratio of leek and konjaku complete stool are: 1:2~3:0.5~1:1~2:1~2:1~2:1~2:2~3:2~3:0.5~1:0.5~1:3~4.
- 4. the preparation method of microbial manure as claimed in claim 1, it is characterised in that in step 2, the matter of ammonia spirit It is 30% to measure fraction.
- 5. the preparation method of microbial manure as claimed in claim 2, it is characterised in that in step 2, hydrogen peroxide solution Mass fraction is 20%.
- 6. the preparation method of microbial manure as claimed in claim 1, it is characterised in that in mix bacterium agent viable count be 1 × 109cfu/g。
- 7. the preparation method of microbial manure as claimed in claim 1, it is characterised in that stalk is crushed to 50~80 mesh.
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CN107628881A (en) * | 2017-10-31 | 2018-01-26 | 博白县富山水果种植专业合作社 | A kind of insect prevention base manure of lichee and preparation method thereof |
CN109020637A (en) * | 2018-08-03 | 2018-12-18 | 淅川县物华生物科技有限公司 | A method of organic fertilizer is produced using stalk |
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