CN107278630B

CN107278630B - Cymbidium floridum nested grafting method

Info

Publication number: CN107278630B
Application number: CN201710589592.9A
Authority: CN
Inventors: 冯金玲; 陈世品; 杨志坚
Original assignee: Fujian Agriculture and Forestry University
Current assignee: Fujian Agriculture and Forestry University
Priority date: 2017-07-19
Filing date: 2017-07-19
Publication date: 2020-03-17
Anticipated expiration: 2037-07-19
Also published as: CN107278630A

Abstract

The invention discloses a cymbidium ensifolium inflorescence nested grafting method, wherein the flowering of the cymbidium ensifolium inflorescence is delayed by refrigeration, the refrigerated cymbidium ensifolium branch scions are treated, nested embedding is carried out in the holes of a stock, a grafting opening is bound by a sealing film, and the moisture and illumination management is enhanced after grafting. The cymbidium ensifolium is cultivated by the method, and the method is verified as follows: the grafting survival rate of the cymbidium ensifolium inflorescence reaches 90-100%, the flowering rate is 100%, the initial flowering phase is 5-7 days after grafting, and the flowering phase lasts for 8-16 days, so that the problem of adjusting the flowering phase of cymbidium ensifolium is solved, and the cymbidium ensifolium inflorescence grafting method has important practical application value.

Description

Cymbidium floridum nested grafting method

Technical Field

The invention relates to a cymbidium floridum inflorescence nested grafting method, and belongs to the field of grafting.

Background

Orchid is a general term of Orchidaceae (Orchidaceae) plants, the Orchidaceae is a most evolved major plant of angiosperms, the orchids have more than 800 genera and more than 25000 species all over the world, and the orchids 173 genera and 1240 species exist in China. Orchid has high appreciation value. Some varieties have peculiar flower type, rich color and thousands of postures; part of varieties are elegant and clean; some varieties are light and elegant in flower fragrance, but they have different flowering periods.

Under the continuous effort of breeders around the world, orchids are ascending in the mainstream rows and columns of ornamental flowers in the world. The orchid industry in China has great development potential and gradually develops into a new industry. However, due to the consumption characteristics of the festivals and holidays of flowers in China, the flower production must meet the supply of the source of the flowers in the festivals and holidays consumption peak period. Environmental factors greatly affect the natural flowering phase of orchids, particularly Chinese orchids, and easily cause the malposition of the flowering phase and market demands, which is very unfavorable for the healthy development of the orchid industry in China. However, the grafting technology is not applied to orchid cultivation at present.

Jianlan (C)Cymbidium ensifolium(Linn.) Sw., also called "Sijilan", which blooms mainly in 6-9 months. The flowering period lasts about 1 week. Is mainly distributed in areas such as south China, east China, southwest China and the like, and has the characteristics of fragrance, high temperature resistance and easiness in flowering. Therefore, the flowering phase of the cymbidium is advanced or delayed by grafting the cymbidium inflorescence, and the individual requirements of consumers on the cymbidium are met.

Disclosure of Invention

In order to adjust the flowering phase of the cymbidium ensifolium, the invention provides a method for nested chimeric grafting of cymbidium ensifolium inflorescences.

The invention comprises the following steps:

(1) cutting unopened cymbidium inflorescence from inflorescence base, packaging with fresh-keeping bag, and storing in refrigerator at 4 deg.C for 0-7 days.

(2) Shearing 2-3 sections of stored cymbidium inflorescence scion, and cutting the lower end of the stored cymbidium inflorescence scion into a nearly conical shape with the length of 2cm by using a small knife.

(3) The inflorescence scions were soaked in 0.2% PVP (polyvinylpyrrolidone) solution for 1-3 minutes.

(4) And selecting a section thicker than the scion as the inflorescence of the rootstock. Cutting off the top tip of the stock, leaving 2cm from the cross section to the first section of the stock, and vertically drilling a hole with the depth of 2cm in the center of the cross section of the stock by using a toothpick.

(5) The cut scion is carefully inserted into the hole of the stock, so that the cut surface at the lower part of the scion is completely inserted into the hole, and the inflorescence scion is inserted into the node position of the stock as much as possible.

(6) The grafting opening is bound by a sealing film. A small bamboo stick is inserted into the flowerpot, and the scion and the stock are fixed by cotton threads.

(7) And (3) sleeving a white transparent freshness protection package on the upper part of the scion, filling cotton at the bag opening along the periphery of the stock during bagging, lightly bundling the cotton by using cotton threads to ensure that the cotton is fully filled with sterile water, and removing the freshness protection package after adding water to 13 days in the morning and evening every day.

(8) Then, the cotton was bagged with a black trash bag, lightly taped over the cotton, and broken after shading for 7 days. Every 2 days, the illumination is enhanced according to the light transmittance of 30 percent, and after the bag is broken for 3 times, the inflorescence is exposed to light completely.

The innovation points of the invention are as follows:

(1) the first cymbidium grafting is successful.

(2) The cymbidium inflorescence is used as a scion and a stock for the first time.

(3) And (5) carrying out cymbidium grafting treatment. The scions were soaked for 1-3 minutes with 0.2% PVP solution which inhibits wound oxidation and promotes callus formation.

(4) The cymbidium inflorescence is grafted by using a nested grafting method for the first time.

The flowers of cymbidium ensifolium are cultivated by the method, and the method is proved to be as follows: the survival rate of the cymbidium ensifolium inflorescence reaches 90-100%, the flowering rate is 100%, the initial flowering phase is 5-7 days after grafting, and the flowering phase lasts for 8-16 days, so that the problem of adjusting the flowering phase of the cymbidium ensifolium is solved, and the method has important practical application value. The method can also be applied to the inflorescences of cymbidium such as cymbidium goeringii, cymbidium faberi, cymbidium kanran and cymbidium sinense.

Detailed Description

The detailed process of the cymbidium ensifolium inflorescence grafting method is described in detail below.

Example 1

(1) Cutting 3 sections of the inflorescence scion of the cymbidium ensifolium directly from the flowerpot, and cutting the lower end of the inflorescence scion into a nearly conical shape with the length of 2cm by a small knife.

(2) Inflorescence scions were soaked in 0.2% PVP solution for 1 minute.

(3) And selecting a section thicker than the scion as the inflorescence of the rootstock. Cutting off the top tip of the stock, leaving about 2cm from the cross section to the first section of the stock, and vertically drilling a hole with the depth of about 2cm in the center of the cross section of the stock by using a toothpick.

(4) The cut scion is carefully inserted into the hole of the stock, so that the cut surface at the lower part of the scion is completely inserted into the hole, and the inflorescence scion is inserted into the node position of the stock as much as possible.

(5) The grafting opening is bound by a sealing film. A small bamboo stick is inserted into the flowerpot, and the scion and the stock are fixed by cotton threads.

(6) And (3) sleeving a white freshness protection package on the upper part of the scion, filling cotton at the bag opening along the periphery of the stock during bagging, lightly bundling the cotton by using cotton threads to ensure that the cotton is fully filled with sterile water, and removing the freshness protection package after adding water to 13 days in the morning and evening every day.

(7) Then, the cotton is covered with a black plastic bag, the cotton is lightly bound, and the bag is broken after 7 days of shade shielding. Every 2 days, the illumination is enhanced according to the light transmittance of 30 percent, and after the bag is broken for 3 times, the flowers are exposed to the full light.

The survival rate of the cymbidium ensifolium inflorescence reaches 100%, the flowering rate is 100%, the initial flowering phase is 5 days after grafting, and the flowering phase lasts for 16 days.

Example 2

(1) Cutting unopened cymbidium inflorescence from inflorescence base, packaging with fresh-keeping bag, and storing in refrigerator at 4 deg.C for 3 days.

(2) 2 sections of the stored cymbidium inflorescence scion are cut, and the lower end of the stored cymbidium inflorescence scion is cut into a nearly conical shape with the length of 2cm by a small knife.

(3) Inflorescence scions were soaked in 0.2% PVP solution for 2 minutes.

(4) And selecting a section thicker than the scion as the inflorescence of the rootstock. Cutting off the top tip of the stock, leaving about 2cm from the cross section to the first section of the stock, and vertically drilling a hole with the depth of about 2cm in the center of the cross section of the stock by using a toothpick.

(7) And (3) sleeving a white freshness protection package on the upper part of the scion, filling cotton at the bag opening along the periphery of the stock during bagging, lightly bundling the cotton by using cotton threads to ensure that the cotton is fully filled with sterile water, and removing the freshness protection package after adding water to 13 days in the morning and evening every day.

(8) Then, the cotton is covered with a black plastic bag, the cotton is lightly bound, and the bag is broken after 7 days of shade shielding. Every 2 days, the illumination is enhanced according to the light transmittance of 30 percent, and after the bag is broken for 3 times, the flowers are exposed to the full light.

The survival rate of the cymbidium ensifolium inflorescence reaches 100%, the flowering rate is 100%, the initial flowering phase is 7 days after grafting, and the flowering phase lasts for 12 days.

Example 3

(1) Shearing unopened cymbidium inflorescence from the base part of the inflorescence, packaging with a fresh-keeping bag, and storing in a refrigerator at 4 deg.C for 7 days;

(2) the stored cymbidium inflorescence scion is cut into 3 sections, and the lower end of the cut cymbidium inflorescence scion is cut into a nearly conical shape with the length of 2cm by a small knife.

(3) Inflorescence scions were soaked in 0.2% PVP solution for 3 minutes.

(8) And covering the cotton with black plastic bags, slightly binding the cotton, and breaking the bags after shading for 7 days. Every 2 days, the illumination is enhanced according to the light transmittance of 30 percent, and after the bag is broken for 3 times, the flowers are exposed to the full light.

The survival rate of the cymbidium ensifolium inflorescence reaches 90%, the flowering rate is 100%, the initial flowering phase is 7 days after grafting, and the flowering phase lasts for 8 days.

The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.

Claims

1. A cymbidium floridum nested grafting method is characterized in that: comprises the following steps:

(1) shearing unopened cymbidium inflorescence from the base part of the inflorescence, packaging with a fresh-keeping bag, and storing in a refrigerator at 4 deg.C for 0-7 days;

(2) shearing 2-3 sections of the stored cymbidium inflorescence scion, and cutting the lower end of the stored cymbidium inflorescence scion into a nearly conical shape with the length of 2cm by using a small knife;

(3) soaking the inflorescence scion in 0.2wt.% PVP solution for 1-3 minutes;

(4) selecting a section thicker than the scion as the inflorescence of the rootstock; cutting off the top tip of the stock, leaving 2cm from the cross section to the first section of the stock, and vertically drilling a hole with the depth of 2cm in the center of the cross section of the stock by using a toothpick;

(5) carefully inserting the cut scion into the hole of the stock to ensure that the cut surface at the lower part of the scion completely enters the hole and the inflorescence scion is inserted into the node position of the stock as much as possible;

(6) wrapping the grafting opening with a sealing film; inserting a small bamboo stick into the flowerpot, and fixing the scion and the stock by cotton threads;

(7) covering a white freshness protection package on the upper part of the scion, filling cotton at the bag opening along the periphery of the stock during bagging, lightly bundling the cotton by using cotton threads to ensure that the cotton is fully filled with sterile water, and removing the freshness protection package after adding water to 13 days in the morning and evening every day;

(8) then, bagging by using a black garbage bag, slightly binding the bag above the cotton, and breaking the bag after shading for 7 days; every 2 days, the illumination is enhanced according to the light transmittance of 30 percent, and after the bag is broken for 3 times, the inflorescence is exposed to light completely.