CN107231946A - A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot - Google Patents
A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot Download PDFInfo
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- CN107231946A CN107231946A CN201710520672.9A CN201710520672A CN107231946A CN 107231946 A CN107231946 A CN 107231946A CN 201710520672 A CN201710520672 A CN 201710520672A CN 107231946 A CN107231946 A CN 107231946A
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- gill fungus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
A kind of method that the present invention discloses application hydrogen rich water raising and the beautiful gill fungus fruiting body yield of stable spot, belongs to fungus growing technique field, and method includes the feature such as the time point of addition hydrogen rich water and the concentration of addition hydrogen rich water.Compared with conventional method, the method is easy to operate, improves to conspicuousness the beautiful gill fungus fruiting body yield of spot.When hydrogen-rich water concentration is 0.8mM, improve the yield 22.23% of low yield bacterial strain and improve the yield 10.38% of superior strain, illustrate that external source addition hydrogen rich water not only increases single bottle yield, also act the effect of high and stable yields, be that the beautiful gill fungus of factorial praluction spot brings being obviously improved for economic benefit.
Description
Technical field
The present invention relates to a kind of raising of application hydrogen rich water and the method for the beautiful gill fungus fruiting body yield of stable spot, belong to edible mushroom cultivation
Train technical field.
Background technology
Spot jade gill fungus（Hypsizygus marmoreus）, also known as crab flavour mushroom, seafood mushroom, letter happiness mushroom, beautiful gill fungus, its taste is fresh
The thick matter of beautiful, meat is tender, mouthfeel is favourable, has saying for " fragrant matsutake, taste is in beautiful gill fungus " in Japan, the green grass or young crops of consumer is also obtained at home
Look at.Due to the unique mouthfeel of the beautiful gill fungus of spot and taste compound, the good reputation of " first is crisp in mushroom " is assigned.Meanwhile, it or one kind are sought
It is foster to be worth very high food medicine dual-purpose bacterium：Rich in 17 kinds of amino acid, wherein 7 kinds are essential amino acid；The Polyphenols thing contained
Matter plays the role of to remove people's interior free yl；Lysine and arginine content are high, have facilitation to the increase of juvenile intelligence；
Polysaccharose substance can improve body immunity, with anti-cancer and the effects such as anticancer.
Spot jade gill fungus is to have been carried out one of Pollution In Edible Mushroomss of factorial praluction, and yield is only second to asparagus, pleurotus eryngii.According to
Edible fungi of china association statistics is shown within 2012, and spot jade gill fungus daily output is up to 167 tons.Spot jade gill fungus is heterothallic quadripolarity load
Daughter bacteria, from the production cycle 105d or so for being inoculated into the harvesting of commodity fructification, than asparagus and pleurotus eryngii long 65d and 45d respectively,
Up to 220g/ bottles when yield is high（1100mL）, less than 100g/ bottles when yielding poorly.Thus, in spot jade gill fungus factorial praluction, the cycle
It is the problem for perplexing enterprise to grow, yield poorly.In face of this situation, if it is beautiful to increase spot by addition derivant outside manual intervention
The yield of gill fungus fructification, is the key for improving the beautiful gill fungus industrial production efficiency of spot.For the edible of medium-scale 5 tons of daily output
For bacterium factory, if averagely improving the beautiful gill fungus fruiting body yield 10% of spot, the economic benefit brought will be very optimistic.
Hydrogen is a nontoxic, tasteless gas, and it can be with active oxygen-hydroxyl free of selective reduction most toxicity
Base, and cell is effectively protected, also, hydrogen does not have the reactive oxygen species of physiological action with other.Hydrogen can conduct
A kind of effective Antioxidation Treatment, because its ability spreads rapidly cross-film, the cellular cytoxicity activity that it can reach and react
Oxygen, so as to protect oxidative damage.In addition, hydrogen is sprouted to mung bean, paddy rice and the seed of clover has material impact；Send out simultaneously
Existing, hydrogen water process can improve the salt stress resistance of paddy rice and arabidopsis.Hydrogen rich gas water process can improve paddy rice and plan
The salt tolerance of southern mustard, and the active oxygen injury that the raising of this salt tolerance may alleviate salt stress induction with hydrogen is relevant.This
Outside, they have found, it is because hydrogen improves the oxidation resistance of plant that hydrogen, which can improve plant resistance to environment stress,.
The content of the invention
The present invention is unstable for fruiting body yield present in the existing cultivation production of the beautiful gill fungus of spot, and fruiting body yield is relatively low,
Biological efficiency is low and the low defect of mushroom house utilization rate, have found it is a kind of can the conspicuousness ground beautiful gill fungus fruiting body yield of stable spot,
The natural materials of its yield and cultivation economic benefit are improved, and the time of hydrogen rich water addition and the optium concentration of addition is determined.
To reach above-mentioned purpose, the technical solution adopted by the present invention is that a kind of application hydrogen rich water is improved and beautiful gill fungus of stable spot
The method of entity yield, comprises the following steps：
The first step：The beautiful gill fungus planting material of spot is put into a plastic bottle, then sterilized；720- is accommodated in the blake bottle
750g spots jade gill fungus planting material；
Second step：The beautiful gill fungus strain of spot is inoculated in the beautiful gill fungus planting material of the spot, bacterium germination room progress darkroom is then placed in and cultivates
To the beautiful gill fungus cultigen of spot；
3rd step：Mycelium stimulation is carried out to the beautiful gill fungus cultigen of the spot, the hydrogen-rich that 10-20ml, concentration are 0.2mM-0.8mM is then added
Water, is next transferred into biochemical cultivation case and carries out mushroom producing culture；
4th step：The bottle cap of the plastic bottle is plucked, then relative humidity be 85-95%, temperature be 13-16 DEG C, illumination be
280-340lx and gas concentration lwevel, which are less than or equal under 2000ppm environmental condition, carries out mushroom producing culture；
5th step：Cultivated 20-24 days in mushroom room, cap not yet deploys, and can be harvested when spore is not yet launched.
It is preferred that technical scheme be：The volume of the plastic bottle is 1000-1200ml.
It is preferred that technical scheme be：The water content of the beautiful gill fungus planting material of the spot is 60-70%.
It is preferred that technical scheme be：The composition of raw materials of the beautiful gill fungus planting material of the spot contains 28-32% wood chip, 30-36%
Corncob, the wheat bran for having 28-32%, 4-6% corn flour and 1-2.5% gypsum.
It is preferred that technical scheme be：It in temperature is 121 DEG C that the condition of the sterilizing, which is, and pressure is to go out under conditions of 0.1MPa
Bacterium 2.5 hours.
It is preferred that technical scheme be：The mycelium stimulation is carried out using mechanical mycelium stimulation equipment, and the mechanical mycelium stimulation equipment is ring-type
After being first 75% alcohol wipe ring-type mycelium stimulation knife with volumetric concentration before mycelium stimulation knife, mycelium stimulation, then ring described in alcohol spraying disinfection is carried out
Shape mycelium stimulation knife.
It is preferred that technical scheme be：In second step, in darkroom, culture obtains the beautiful gill fungus cultigen of spot for 80-90 days；The hair
The temperature of bacterium room is 23-27 DEG C.
Because above-mentioned technical proposal is used, the present invention has following advantages and effect compared with prior art：
The present invention method with the past improve edible mushroom yield method compared with, its is easy to operate, and effect is more prominent, it is safer can
Lean on, improve the utilization rate of mushroom house and add economic benefit.This method is only needed to after mycelium stimulation, adds certain density hydrogen-rich
Water, you can to improve the beautiful gill fungus fruiting body yield of spot.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment one：A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot
1st, the preparation of planting material
The formula of spot jade gill fungus planting material is：30% wood chip, 33% corncob, 30% wheat bran, 5% corn flour and 2% gypsum.Wherein
Corncob is prewetted half an hour in advance, and the water content of compost is 65% or so.Plastic bottle volume is 1100ml, the amount per bottled material
Between 720-750g, then at 121 DEG C, pressure is sterilized 2.5 hours under 0.1MPa.
2nd, the inoculation and culture of Cultivar culture medium
By Cultivar culture medium beautiful gill fungus bacterial strain SIEFF3153 and SIEFF3154 the liquid spawn 10ml of access spot in step 2, put
Enter bacterium germination room and 85 days are cultivated in 25 DEG C or so darkrooms as the beautiful gill fungus cultigen of spot.
3rd, mycelium stimulation
The cultigen for growing into 85 days in step 3 is subjected to mycelium stimulation, old mycelia is scratched, then addition 15mL concentration is respectively
0.2mM hydrogen rich water, is transferred into biochemical cultivation case and carries out mushroom producing culture.
4th, mushroom producing culture
Cultigen after mycelium stimulation in step 4 is put into biochemical cultivation case, bottle cap is plucked, in relative humidity 85%-95%, temperature
13-16 DEG C, illumination 300lx or so and carbon dioxide carry out mushroom producing culture in below 2000ppm.After testing, carbon dioxide is specific
For 1800ppm.
5th, harvesting and data statistics
When cultigen is cultivated about 22 days or so in biochemical cultivation case, cap not yet deploys, optimum harvest when spore is not yet launched.
Every bottle of fructification is weighed, average every bottle yield is calculated.
Embodiment two：A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot
1st, the preparation of planting material
The formula of spot jade gill fungus planting material is：30% wood chip, 33% corncob, 30% wheat bran, 5% corn flour and 2% gypsum.Wherein
Corncob is prewetted half an hour in advance, and the water content of compost is 65% or so.Plastic bottle volume is 1100mL, the amount per bottled material
Between 720-750g, then at 121 DEG C, pressure is sterilized 2.5 hours under 0.1MPa.
2nd, the inoculation and culture of Cultivar culture medium
By Cultivar culture medium beautiful gill fungus bacterial strain SIEFF3153 and SIEFF3154 the liquid spawn 10ml of access spot in step 2, put
Enter bacterium germination room and 85 days are cultivated in 25 DEG C or so darkrooms as the beautiful gill fungus cultigen of spot.
3rd, mycelium stimulation
The cultigen for growing into 85 days in step 3 is subjected to mycelium stimulation, old mycelia is scratched, then addition 15ml concentration is respectively
0.4mM hydrogen rich water, is transferred into biochemical cultivation case and carries out mushroom producing culture.
4th, mushroom producing culture
Cultigen after mycelium stimulation in step 4 is put into biochemical cultivation case, bottle cap is plucked, in relative humidity 85%-95%, temperature
13-16 DEG C, illumination 300lx or so and carbon dioxide carry out mushroom producing culture in below 2000ppm.After testing, carbon dioxide is specific
For 1700ppm.
5th, harvesting and data statistics
When cultigen is cultivated about 22 days or so in biochemical cultivation case, cap not yet deploys, optimum harvest when spore is not yet launched.
Every bottle of fructification is weighed, average every bottle yield is calculated.
Embodiment three：A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot
1st, the preparation of planting material
The formula of spot jade gill fungus planting material is：30% wood chip, 33% corncob, 30% wheat bran, 5% corn flour and 2% gypsum.Wherein
Corncob is prewetted half an hour in advance, and the water content of compost is 65% or so.Plastic bottle volume is 1100mL, the amount per bottled material
Between 720-750g, then at 121 DEG C, pressure is sterilized 2.5 hours under 0.1MPa.
2nd, the inoculation and culture of Cultivar culture medium
By Cultivar culture medium beautiful gill fungus bacterial strain SIEFF3153 and SIEFF3154 the liquid spawn 10ml of access spot in step 2, put
Enter bacterium germination room and 85 days are cultivated in 25 DEG C or so darkrooms as the beautiful gill fungus cultigen of spot.
3rd, mycelium stimulation
The cultigen for growing into 85 days in step 3 is subjected to mycelium stimulation, old mycelia is scratched, then addition 15ml concentration is respectively
0.8mM hydrogen rich water, is transferred into biochemical cultivation case and carries out mushroom producing culture.
4th, mushroom producing culture
Cultigen after mycelium stimulation in step 4 is put into biochemical cultivation case, bottle cap is plucked, in relative humidity 85%-95%, temperature
13-16 DEG C, illumination 300lx or so and carbon dioxide carry out mushroom producing culture in below 2000ppm.After testing, carbon dioxide is specific
For 1850ppm.
5th, harvesting and data statistics
When cultigen is cultivated about 22 days or so in biochemical cultivation case, cap not yet deploys, optimum harvest when spore is not yet launched.
Every bottle of fructification is weighed, average every bottle yield is calculated.
Comparative example：
1st, the preparation of planting material
The formula of spot jade gill fungus planting material is：30% wood chip, 33% corncob, 30% wheat bran, 5% corn flour and 2% gypsum.Wherein
Corncob is prewetted half an hour in advance, and the water content of compost is 65% or so.Plastic bottle volume is 1100ml, the amount per bottled material
Between 720-750g, then at 121 DEG C, pressure is sterilized 2.5 hours under 0.1MPa.
2nd, the inoculation and culture of Cultivar culture medium
By Cultivar culture medium beautiful gill fungus bacterial strain SIEFF3153 and SIEFF3154 the liquid spawn 10ml of access spot in step 2, put
Enter bacterium germination room and 85 days are cultivated in 25 DEG C or so darkrooms as the beautiful gill fungus cultigen of spot.
3rd, mycelium stimulation
The cultigen for growing into 85 days in step 3 is subjected to mycelium stimulation, old mycelia, then addition 15ml distilled water respectively is scratched,
It is transferred into biochemical cultivation case and carries out mushroom producing culture.
4th, mushroom producing culture
Cultigen after mycelium stimulation in step 4 is put into biochemical cultivation case, bottle cap is plucked, in relative humidity 85%-95%, temperature
13-16 DEG C, illumination 300lx or so and carbon dioxide carry out mushroom producing culture in below 2000ppm.
5th, harvesting and data statistics
When cultigen is cultivated about 22 days or so in biochemical cultivation case, cap not yet deploys, optimum harvest when spore is not yet launched.
Every bottle of fructification is weighed, average every bottle yield is calculated.
As a result it is as shown in the table：
| Strain name | 0mM HRW | 0.2mM HRW | 0.4mM HRW | 0.8mM HRW |
| SIEF3153 | 162.17±10.90 | 178.29±12.67 | 179.67±14.12 | 198.22±11.09 |
| SIEF3154 | 252.17±5.90 | 272.47±17.23 | 271.33±11.37 | 278.36±13.58 |
The hydrogen rich water of the present invention improves the application of the beautiful gill fungus fruiting body yield of spot, is carried out in the factory of the beautiful gill fungus of factorial praluction spot
, it is that the cultigen of 85 days is complete in mechanical mycelium stimulation respectively to incubation time, adds the hydrogen rich water of various concentrations, compares and add with control
Plus the experimental group fruiting body yield conspicuousness of hydrogen rich water（P<0.05）Carried to the beautiful gill fungus fruiting body yield conspicuousness of increase, i.e. spot
Height, and hydrogen rich water is more notable to low-producing bacterial strain effect of increasing production.
When hydrogen-rich water concentration reaches 0.8mM, yield 10.38% can be improved to superior strain SIEF3154, for low yield
Bacterial strain SIEF3153 can improve yield 22.23%.Various concentrations hydrogen rich water on SIEF3153 producing strains influence variation tendency with
SIEF3154 bacterial strains have uniformity；But, amount of increase in production is substantially higher than SIEF3154 bacterial strain.This phenomenon, one side explanation
The conclusion that hydrogen rich water influences on SIEF3154 producing strains；On the other hand, hydrogen rich water can also be illustrated to that will be washed in a pan due to degenerating
The SIEF3153 bacterial strain action effects eliminated are more notable, can maintain the beautiful gill fungus yield of spot in higher level.This is in the past on the beautiful gill fungus of spot
Research in the effect that is unable to reach.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand present disclosure and implement according to this, and it is not intended to limit the scope of the present invention.It is all according to the present invention
The equivalent change or modification that Spirit Essence is made, should all be included within the scope of the present invention.
Claims (7)
1. a kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot, it is characterised in that：Comprise the following steps：
The first step：The beautiful gill fungus planting material of spot is put into a plastic bottle, then sterilized；720- is accommodated in the blake bottle
750g spots jade gill fungus planting material；
Second step：The beautiful gill fungus strain of spot is inoculated in the beautiful gill fungus planting material of the spot, bacterium germination room progress darkroom is then placed in and cultivates
To the beautiful gill fungus cultigen of spot；
3rd step：Mycelium stimulation is carried out to the beautiful gill fungus cultigen of the spot, the hydrogen-rich that 10-20ml, concentration are 0.2mM-0.8mM is then added
Water, is next transferred into biochemical cultivation case and carries out mushroom producing culture；
4th step：The bottle cap of the plastic bottle is plucked, then relative humidity be 85-95%, temperature be 13-16 DEG C, illumination be
280-340lx and gas concentration lwevel, which are less than or equal under 2000ppm environmental condition, carries out mushroom producing culture；
5th step：Cultivated 20-24 days in mushroom room, cap not yet deploys, and can be harvested when spore is not yet launched.
2. application hydrogen rich water raising according to claim 1 and the method for the beautiful gill fungus fruiting body yield of stable spot, its feature exist
In：The volume of the plastic bottle is 1000-1200mL.
3. application hydrogen rich water raising according to claim 1 and the method for the beautiful gill fungus fruiting body yield of stable spot, its feature exist
In：The water content of the beautiful gill fungus planting material of the spot is 60-70%.
4. application hydrogen rich water raising according to claim 1 and the method for the beautiful gill fungus fruiting body yield of stable spot, its feature exist
In：The composition of raw materials of the beautiful gill fungus planting material of the spot contain 28-32% wood chip, 30-36% corncob, the wheat bran for having 28-32%,
4-6% corn flour and 1-2.5% gypsum.
5. application hydrogen rich water raising according to claim 1 and the method for the beautiful gill fungus fruiting body yield of stable spot, its feature exist
In：The condition of the sterilizing is is 121 DEG C in temperature, and pressure is sterilizing 2.5 hours under conditions of 0.1MPa.
6. application hydrogen rich water extension spot beautiful gill fungus shelf time according to claim 1 and the method for improving its quality, it is special
Levy and be：The mycelium stimulation is carried out using mechanical mycelium stimulation equipment, and the mechanical mycelium stimulation equipment is first used before being ring-type mycelium stimulation knife, mycelium stimulation
After volumetric concentration is 75% alcohol wipe ring-type mycelium stimulation knife, then ring-type mycelium stimulation knife described in alcohol spraying disinfection is carried out.
7. application hydrogen rich water extension spot beautiful gill fungus shelf time according to claim 1 and the method for improving its quality, it is special
Levy and be：In second step, in darkroom, culture obtains the beautiful gill fungus cultigen of spot for 80-90 days；The temperature of the bacterium germination room is 23-27
℃。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063204A (en) * | 2019-06-04 | 2019-07-30 | 上海市农业科学院 | A method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide |
| CN110089349A (en) * | 2019-06-04 | 2019-08-06 | 上海市农业科学院 | A method of spot jade gill fungus fruiting body yield is improved using vitamin C |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106818209A (en) * | 2017-01-23 | 2017-06-13 | 上海市农业科学院 | A kind of application hydrogen rich water increases the method and its processing step in the degeneration-resistant border of spot jade gill fungus mycelia |
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2017
- 2017-06-30 CN CN201710520672.9A patent/CN107231946A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818209A (en) * | 2017-01-23 | 2017-06-13 | 上海市农业科学院 | A kind of application hydrogen rich water increases the method and its processing step in the degeneration-resistant border of spot jade gill fungus mycelia |
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| 关良洲等编著: "《真姬菇优质高产栽培技术》", 31 January 2008, 成都：天地出版社 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110063204A (en) * | 2019-06-04 | 2019-07-30 | 上海市农业科学院 | A method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide |
| CN110089349A (en) * | 2019-06-04 | 2019-08-06 | 上海市农业科学院 | A method of spot jade gill fungus fruiting body yield is improved using vitamin C |
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