CN110089349A - A method of spot jade gill fungus fruiting body yield is improved using vitamin C - Google Patents
A method of spot jade gill fungus fruiting body yield is improved using vitamin C Download PDFInfo
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- CN110089349A CN110089349A CN201910481593.0A CN201910481593A CN110089349A CN 110089349 A CN110089349 A CN 110089349A CN 201910481593 A CN201910481593 A CN 201910481593A CN 110089349 A CN110089349 A CN 110089349A
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- vitamin
- gill fungus
- spot jade
- jade gill
- fruiting body
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention discloses a kind of method for improving spot jade gill fungus fruiting body yield using vitamin C, belongs to technical field of edible fungi cultivation.Method includes the screening of vitamin C concentration, the screening of cultural hypha time and addition ascorbic time point.It is compared with the traditional method, the method is easy to operate, improves to conspicuousness spot jade gill fungus fruiting body yield.When vitamin C concentration is 400mg/L, improving spot jade gill fungus fruiting body yield most significantly is 9.75%, illustrates that single bottle yield has can be improved in external source addition vitamin C；When the cultural hypha time is 80 days, can maximally improve spot jade gill fungus fruiting body yield is 9.85%.These results suggest that being 80 days in the cultural hypha time, when vitamin C concentration is 400mg/L, vitamin C effect of increasing production is most significant, is that the factorial production spot jade gill fungus bring economic benefit is most significant.
Description
Technical field
The present invention relates to a kind of methods for improving spot jade gill fungus fruiting body yield using vitamin C, belong to edible fungus culturing skill
Art field.
Background technique
Spot jade gill fungus (Hypsizygus marmoreus) also known as crab flavour mushroom, seafood mushroom, letter happiness mushroom, beautiful gill fungus, taste are fresh
Beauty, meat thickness matter is tender, and mouthfeel is favourable, has saying for " for perfume in matsutake, taste being in beautiful gill fungus " in Japan, also obtains the blueness of consumer at home
It looks at.Due to the unique mouthfeel of spot jade gill fungus and taste compound, it is assigned the good reputation of " first is crisp in mushroom ".Spot jade gill fungus is to have been carried out work
One of the Pollution In Edible Mushroomss that factory's metaplasia produces, yield is only second to needle mushroom, Pleurotus eryngii.According to edible fungi of china association statistical number in 2016
According to display, spot jade gill fungus is produced daily up to 608 tons.Spot jade gill fungus is heterothallic quadripolarity basidiomycetes, is adopted from commodity fructification is inoculated into
Production cycle 105d of receipts or so, respectively than needle mushroom and Pleurotus eryngii long 65d and 45d, up to 220g/ bottles when yield is high
(1100mL), less than 100g/ bottles when low output.Thus, in spot jade gill fungus the factorial production, the period is long, and low output causes spot jade gill fungus
The factorial production it is relatively inefficient, be perplex enterprise problem.In face of this situation, if by adding outside manual intervention
Add inducer to increase the yield of spot jade gill fungus fructification, is the key that improve spot jade gill fungus industrial production efficiency.
Vitamin C has strong reducing property, is a kind of typical antioxidant.Ascorbic anti-oxidation function mainly shows
Oxidation material or oxidizer free radicals can be reduced at it, can Scavenger of ROS, alleviate injury of the oxygen radical to cell.Dimension life
Plain C is also a kind of required nutrient, in a variety of hydroxylatings and maintains the organelle including mitochondria and endoplasmic reticulum
In Redox homeostasis in play key effect.But relevant report is had no in terms of edible fungus production increasing.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for improving spot jade gill fungus fruiting body yield using vitamin C.
In order to achieve the above objects and other related objects, present invention provide the technical scheme that a kind of application vitamin C mentions
The method of high spot jade gill fungus fruiting body yield, including the following steps:
Step 1: spot jade gill fungus the factorial production being inoculated in culture material with strain, then carries out dark culture；
Step 2: cultivating to after 40-120 days, carry out mycelium stimulation, and add the concentration of 15ml to culture material as 100-800mg/L
Vitamin C aqueous solution；
Step 3: being then transferred into mushroom room and carry out mushroom producing culture.
Preferred technical solution are as follows: the composition of raw materials of the culture material includes the raw material of following mass percent: 28-32%
Sawdust, 30-35% corncob, 28-30% wheat bran, 4-6% corn flour and 1.5-2.5% gypsum.
Preferred technical solution are as follows: the half an hour of the corncob before the use is prewetted in advance；The culture material
Water content be 60-70%；The culture material is before inoculation, and using 121 DEG C, the parameter of pressure 0.1Mpa sterilizes 2.5 hours.
Preferred technical solution are as follows: every 720-750g culture material is inoculated with spot jade gill fungus the factorial production strain 10ml.
Preferred technical solution are as follows: the concentration of hydrogen peroxide is 400mg/L.
Preferred technical solution are as follows: the condition of mushroom producing culture are as follows: relative humidity 85%-95%, temperature be 13-16 DEG C,
Illumination is 250-350lx, carbon dioxide is less than or equal to 3000ppm.
Preferred technical solution are as follows: step 2: cultivating to after 80-85 days, carries out mycelium stimulation, and to culture material addition 15ml's
Concentration is the hydrogen peroxide of 400mg/L.
Preferred technical solution are as follows: temperature when dark culture is 23-27 DEG C.
Since above-mentioned technical proposal is used, the present invention has the advantage, that compared with prior art
For the method for the present invention compared with the method for improving edible mushroom yield in the past, easy to operate, effect is more prominent, safer
Reliably, it improves the utilization rate of mushroom house and increases economic benefit.This method only needs after mycelium stimulation, adds certain density dimension
Raw element C, can be improved spot jade gill fungus fruiting body yield, and when vitamin C concentration reaches 400mg/L in the cultural hypha time,
Yield-increasing efficiency reaches up to 9.75%.In the different cultural hypha times, when the cultural hypha time is 80 days, yield-increasing efficiency reaches
Highest is 9.86%.These results suggest that on the one hand illustrating that vitamin C improves spot jade gill fungus fruiting body yield；On the other hand explanation
Vitamin C shortens spot jade gill fungus cultivation period.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
The term of such as "upper", "lower", "left", "right", " centre " and " one " cited in this specification, is also only just
In being illustrated for narration, rather than to limit the scope of the invention, relativeness is altered or modified, without substantive change
Under more technology contents, when being also considered as the enforceable scope of the present invention.
Embodiment 1: a method of spot jade gill fungus fruiting body yield is improved using vitamin C
1, the preparation of culture material
The formula of spot jade gill fungus culture material is: 30% sawdust, 33% corncob, 30% wheat bran, 5% corn flour and 2% gypsum.
Wherein corncob is prewetted half an hour in advance, the water content of compost is 65% or so.Plastic bottle volume be 1100ml, every bottle
The amount of charging is between 720-750g, and then at 121 DEG C, pressure is sterilized 2.5 hours under 0.1MPa.
2, the inoculation and culture of Cultivar culture medium
Cultivar culture medium in step 2 is accessed into spot jade gill fungus batch production strain liquid strain 10ml, bacteria-producing room is put into and exists
25 DEG C or so darkroom cultures, carry out fruiting experiment for the compost grown.
3, different vitamin C concentration experiments
The cultivar for growing into 85 days in step 2 is subjected to mycelium stimulation, scratches old mycelia, then add 100mg/L respectively,
The vitamin C and distilled water 15ml (comparative example) of 200mg/L, 400mg/L and 800mg/L, the fertility being transferred into factory
Mushroom producing culture is carried out in room.
4, mushroom producing culture
Cultivation condition are as follows: humidity 85%-95%, 13-16 DEG C of temperature, illumination 300lx or so and carbon dioxide are in 3000ppm
Mushroom producing culture is carried out below.The gas concentration lwevel of the present embodiment is specially 2500ppm.
5 harvestings and data statistics
When cultivar is cultivated about 22 days or so in biochemical cultivation case, cap is not yet unfolded, optimum period when spore not yet launches
Harvesting.Every bottle of fructification is weighed, average every bottle of yield (table 1) is calculated.As shown in Table 1, when vitamin C concentration is
When 400mg/L, yield-increasing efficiency is maximum.
Table 1 is influence of the different vitamin C concentrations to spot jade gill fungus yield.
Embodiment 2: a method of spot jade gill fungus fruiting body yield is improved using vitamin C
1, the preparation of culture material
The formula of spot jade gill fungus culture material is: 30% sawdust, 33% corncob, 30% wheat bran, 5% corn flour and 2% gypsum.
Wherein corncob is prewetted half an hour in advance, the water content of compost is 65% or so.Plastic bottle volume be 1100ml, every bottle
The amount of charging is between 720-750g, and then at 121 DEG C, pressure is sterilized 2.5 hours under 0.1MPa.
2, the inoculation and culture of Cultivar culture medium
Cultivar culture medium in step 2 is accessed into spot jade gill fungus batch production strain liquid strain 10ml, bacteria-producing room is put into and exists
25 DEG C or so darkroom cultures, carry out fruiting experiment for the compost grown.
3, different cultural hypha time-triggered protocols
The cultivar for growing into 40 days, 60 days, 80 days, 100 days and 120 days in step 2 is subjected to mycelium stimulation, scratches old bacterium
Silk, then the vitamin C and distilled water 15ml of 400mg/L is added, it is transferred into the fertility room in factory and carries out mushroom producing culture.
4, mushroom producing culture
Cultivation condition are as follows: humidity 85%-95%, 13-16 DEG C of temperature, illumination 300lx or so and carbon dioxide are in titanium dioxide
Carbon carries out mushroom producing culture in 3000ppm or less.The gas concentration lwevel of the present embodiment is specially 2600ppm.
5, harvesting and data statistics
When cultivating about 22 days or so in the fertility room of cultivar in the factory, cap is not yet unfolded, when spore not yet launches
Optimum harvest.Every bottle of fructification is weighed, average every bottle of yield (table 2) is calculated.As shown in Table 2, work as cultural hypha
When time is 80 days, it has been more than the control group that the cultural hypha time is 100 days and 120 days that external source, which adds ascorbic yield,
Yield illustrates that external source addition vitamin C shortens spot jade gill fungus cultivation period.
Table 2 is influence of the different cultural hypha times respectively to spot jade gill fungus yield.
Embodiment 3: a method of spot jade gill fungus fruiting body yield is improved using vitamin C
1, vitamin C improve and stablize spot jade gill fungus yield method, it is characterised in that select low yield and superior strain as
The time point of research object, vitamin C addition selects after mycelium stimulation, and adding ascorbic optium concentration is 400mg/L, addition
The ascorbic best cultural hypha time is 80 days.
2, the method for vitamin C of the invention to spot jade gill fungus volume increase stable yields, including the following steps:
(1) industrial bottle carrying agent system
Spot jade gill fungus cultivation system is: plastic bottle volume is 1100ml, and cultivation wet feed is 720-750g/ bottles.
(2) research object
The bacterial strain of choice experiment is the normal production bacterial strain of batch production, and used is the substance dimension life with reproducibility
Plain C.
(2) mycelium stimulation and mushroom producing culture
Incubation time in step (1) is added after 80 days cultivar mycelium stimulations by the time point selection of vitamin C addition
Vitamin C.Mycelium stimulation is carried out using mechanical mycelium stimulation equipment, then adds vitamin C, vitamin C and the distillation that concentration is 400mg/L
Water 15ml transfers it to mushroom room and carries out mushroom producing culture.
(3) harvesting and data statistics
When cultivar is cultivated about 22 days in mushroom room, cap is not yet unfolded, optimum harvest when spore not yet launches.It will be every
The fructification of bottle is weighed, and average every bottle of yield is calculated.
The bacterial strain selected in step (2) for spot jade gill fungus the factorial production normal strains.
The ascorbic cultivar of the addition best cultural hypha time selected in step (2) is 80 days
The ascorbic optium concentration of addition selected in step (2) is 400mg/L
The ascorbic time point selection of addition is after mycelium stimulation in step (2), i.e., grows into the 80th day from being inoculated into mycelia
Certain density vitamin C is added after carrying out mycelium stimulation.
Substance with reproducibility used in step (2), which also has to have, to be increased spot jade gill fungus fruiting body yield and shortens week
The function of phase.
Vitamin C improves spot jade gill fungus yield and shortens the application of spot jade gill fungus cultivation period, is in the factorial production spot jade gill fungus
Factory in carry out, the bacterial strain of selection is the normal strains of the factorial production, primarily looked at the vitamin C of various concentration
(100mg/L, 200mg/L, 400mg/L and 800mg/L) has found that effect of increasing production is most in 400mg/L to spot jade gill fungus yield increasing effect
Significantly, reach 9.75%；Then, ascorbic yield increasing effect under different cultural hypha times (40-120 days) has been investigated, has been found
Spot jade gill fungus fruiting body yield can be improved significantly when incubation time is 80 days, reach 9.86%, when being more than cultural hypha
Between for 100 days and 120 days control groups yield, illustrate that external source adds the cultivation period that vitamin C shortens spot jade gill fungus.
Embodiment 4: a method of spot jade gill fungus fruiting body yield is improved using vitamin C
A method of spot jade gill fungus fruiting body yield is improved using vitamin C, including the following steps:
Step 1: spot jade gill fungus the factorial production being inoculated in culture material with strain, then carries out dark culture；
Step 2: cultivating to after 40 days, carry out mycelium stimulation, and the vitamin for being 100mg/L to the concentration of culture material addition 15ml
C aqueous solution；
Step 3: being then transferred into mushroom room and carry out mushroom producing culture.
Preferred embodiment are as follows: the composition of raw materials of the culture material includes the raw material of following mass percent: 30.5%
Sawdust, 35% corncob, 28% wheat bran, 4% corn flour and 2.5% gypsum.
Preferred embodiment are as follows: the half an hour of the corncob before the use is prewetted in advance；The culture material
Water content be 60%；The culture material is before inoculation, and using 121 DEG C, the parameter of pressure 0.1Mpa sterilizes 2.5 hours.
Preferred embodiment are as follows: every 735g culture material is inoculated with spot jade gill fungus the factorial production strain 10ml.
Preferred embodiment are as follows: the concentration of hydrogen peroxide is 400mg/L.
Preferred embodiment are as follows: the condition of mushroom producing culture are as follows: relative humidity 85%%, temperature are 16 DEG C, illumination is
350lx, gas concentration lwevel are specially 2700ppm.
Preferred embodiment are as follows: step 2: cultivating to after 85 days, carries out mycelium stimulation, and the concentration of 15ml is added to culture material
For the hydrogen peroxide of 400mg/L.
Preferred embodiment are as follows: temperature when dark culture is 27 DEG C.
As described above is only to be not intended to tool to explain the preferred embodiments of the invention to do any shape to the present invention
Limitation in formula should all wrap therefore all have any modification or change for making the related present invention under identical spirit
It includes in the scope that the invention is intended to protect.
Claims (8)
1. a kind of method for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: include the following steps:
Step 1: spot jade gill fungus the factorial production being inoculated in culture material with strain, then carries out dark culture；
Step 2: cultivating to after 40-120 days, carry out mycelium stimulation, and the dimension for being 100-800mg/L to the concentration of culture material addition 15ml
Raw element C aqueous solution；
Step 3: being then transferred into mushroom room and carry out mushroom producing culture.
2. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: institute
The composition of raw materials for stating culture material includes the raw material of following mass percent: 28-32% sawdust, 30-35% corncob, 28-30% bran
Skin, 4-6% corn flour and 1.5-2.5% gypsum.
3. the method according to claim 2 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: institute
The half an hour of corncob before the use is stated to be prewetted in advance；The water content of the culture material is 60-70%；The culture material
Before inoculation, using 121 DEG C, the parameter of pressure 0.1Mpa sterilizes 2.5 hours.
4. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: every
720-750g culture material is inoculated with spot jade gill fungus the factorial production strain 10ml.
5. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: double
The concentration of oxygen water is 400mg/L.
6. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: go out
Mushroom culture condition are as follows: relative humidity 85%-95%, temperature are 13-16 DEG C, illumination 250-350lx, carbon dioxide are less than or
Equal to 3000ppm.
7. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: step
Rapid 2: cultivating to after 80-85 days, carry out mycelium stimulation, and the hydrogen peroxide for being 400mg/L to the concentration of culture material addition 15ml.
8. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using vitamin C, it is characterised in that: dark
Temperature when culture is 23-27 DEG C.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069189A (en) * | 2016-06-21 | 2016-11-09 | 桐城市佳明农业发展有限公司 | A kind of industrial culture method of seafood mushroom |
| CN107118970A (en) * | 2017-04-28 | 2017-09-01 | 福建农林大学 | A kind of activating agent for promoting ganoderma lucidum to grow |
| CN107231946A (en) * | 2017-06-30 | 2017-10-10 | 上海市农业科学院 | A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot |
| CN109463200A (en) * | 2018-12-24 | 2019-03-15 | 贵州省贵福菌业发展有限公司 | A kind of oil tea mushroom cultivation method |
-
2019
- 2019-06-04 CN CN201910481593.0A patent/CN110089349A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069189A (en) * | 2016-06-21 | 2016-11-09 | 桐城市佳明农业发展有限公司 | A kind of industrial culture method of seafood mushroom |
| CN107118970A (en) * | 2017-04-28 | 2017-09-01 | 福建农林大学 | A kind of activating agent for promoting ganoderma lucidum to grow |
| CN107231946A (en) * | 2017-06-30 | 2017-10-10 | 上海市农业科学院 | A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot |
| CN109463200A (en) * | 2018-12-24 | 2019-03-15 | 贵州省贵福菌业发展有限公司 | A kind of oil tea mushroom cultivation method |
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