CN110063204A - A method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide - Google Patents
A method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide Download PDFInfo
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- CN110063204A CN110063204A CN201910481592.6A CN201910481592A CN110063204A CN 110063204 A CN110063204 A CN 110063204A CN 201910481592 A CN201910481592 A CN 201910481592A CN 110063204 A CN110063204 A CN 110063204A
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- Prior art keywords
- hydrogen peroxide
- gill fungus
- spot jade
- jade gill
- fruiting body
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
Abstract
The present invention discloses a kind of method for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, belongs to technical field of edible fungi cultivation.Method includes the screening of concentration of hydrogen peroxide, the screening of cultural hypha time and the time point for adding hydrogen peroxide.It is compared with the traditional method, the method is easy to operate, improves to conspicuousness spot jade gill fungus fruiting body yield.When concentration of hydrogen peroxide is 4mM, improving spot jade gill fungus fruiting body yield most significantly is 6.44%, illustrates that single bottle yield has can be improved in external source addition hydrogen peroxide；When the cultural hypha time is 80 days, can maximally improve spot jade gill fungus fruiting body yield is 8.19%.These results suggest that being 80 days in the cultural hypha time, when concentration of hydrogen peroxide is 4mM, hydrogen peroxide effect of increasing production is most significant, while can shorten spot jade gill fungus cultivation period, is that the factorial production spot jade gill fungus bring economic benefit is most significant.
Description
Technical field
The present invention relates to a kind of methods for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, belong to edible fungus culturing skill
Art field.
Background technique
Spot jade gill fungus (Hypsizygus marmoreus) also known as crab flavour mushroom, seafood mushroom, letter happiness mushroom, beautiful gill fungus, taste are fresh
Beauty, meat thickness matter is tender, and mouthfeel is favourable, has saying for " for perfume in matsutake, taste being in beautiful gill fungus " in Japan, also obtains the blueness of consumer at home
It looks at.Due to the unique mouthfeel of spot jade gill fungus and taste compound, it is assigned the good reputation of " first is crisp in mushroom ".Spot jade gill fungus is to have been carried out work
One of the Pollution In Edible Mushroomss that factory's metaplasia produces, yield is only second to needle mushroom, Pleurotus eryngii.According to edible fungi of china association statistical number in 2016
According to display, spot jade gill fungus is produced daily up to 608 tons.
Spot jade gill fungus is heterothallic quadripolarity basidiomycetes, and from being inoculated into, production cycle 105d that commodity fructification harvests is left
The right side, long 65d and 45d respectively than needle mushroom and Pleurotus eryngii, up to 220g/ bottles (1100mL) when yield is high, when low output, is insufficient
100g/ bottles.Thus, in spot jade gill fungus the factorial production, the period is long, and low output causes the factorial production efficiency of spot jade gill fungus relatively
It is low, it is the problem for perplexing enterprise.In face of this situation, if it is real to increase spot jade gill fungus by addition inducer outside manual intervention
The yield of body is the key that improve spot jade gill fungus industrial production efficiency.
Hydrogen peroxide (H2O2) it is a kind of typical strong oxidizer, aqueous solution is suitable for being disappeared with wound disinfection and environment
Poison and food sterilization, hydrogen peroxide can resolve into water and oxygen under normal circumstances.But the research in edible mushroom does not appear in the newspapers
Road.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for improving spot jade gill fungus fruiting body yield using hydrogen peroxide.
In order to achieve the above objects and other related objects, present invention provide the technical scheme that a kind of apply hydrogen peroxide
The method for improving spot jade gill fungus fruiting body yield, including the following steps:
Step 1: spot jade gill fungus the factorial production being inoculated in culture material with strain, then carries out dark culture；
Step 2: cultivating to after 60-120 days, carry out mycelium stimulation, and the dioxygen for being 2-8mM to the concentration of culture material addition 15ml
Water；
Step 3: being then transferred into mushroom room and carry out mushroom producing culture.
Preferred technical solution are as follows: the composition of raw materials of the culture material includes the raw material of following mass percent: 28-32%
Sawdust, 30-35% corncob, 28-30% wheat bran, 4-6% corn flour and 1.5-2.5% gypsum.
Preferred technical solution are as follows: the half an hour of the corncob before the use is prewetted in advance；The culture material
Water content be 60-70%；The culture material is before inoculation, and using 121 DEG C, the parameter of pressure 0.1Mpa sterilizes 2.5 hours.
Preferred technical solution are as follows: every 720-750g culture material is inoculated with spot jade gill fungus the factorial production strain 10ml.
Preferred technical solution are as follows: the concentration of hydrogen peroxide is 4mM.
Preferred technical solution are as follows: the condition of mushroom producing culture are as follows: relative humidity 85%-95%, temperature be 13-16 DEG C,
Illumination is 250-350lx, carbon dioxide is less than or equal to 3000ppm.
Preferred technical solution are as follows: step 2: cultivating to after 80-85 days, carries out mycelium stimulation, and to culture material addition 15ml's
Concentration is the hydrogen peroxide of 4mM.
Preferred technical solution are as follows: temperature when dark culture is 23-27 DEG C.
Since above-mentioned technical proposal is used, the present invention has the advantage, that compared with prior art
The present invention in the past improve edible mushroom yield method compared with, easy to operate, effect is more prominent, it is safer can
It leans on, improve the utilization rate of mushroom house and increases economic benefit.This method only needs after mycelium stimulation, adds certain density peroxide
Change hydrogen, spot jade gill fungus fruiting body yield can be improved, and when concentration of hydrogen peroxide reaches 4mM, yield-increasing efficiency reaches up to
6.44%.In the different cultural hypha times, when the cultural hypha time is 80 days, yield-increasing efficiency adds peroxidating up to 8.19%
The yield of hydrogen group has been more than the yield for the cultural hypha time being 100 days and 120 days.It can illustrate: on the one hand illustrate hydrogen peroxide
Improve spot jade gill fungus fruiting body yield；On the other hand illustrate that hydrogen peroxide shortens spot jade gill fungus cultivation period.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
The term of such as "upper", "lower", "left", "right", " centre " and " one " cited in this specification, is also only just
In being illustrated for narration, rather than to limit the scope of the invention, relativeness is altered or modified, without substantive change
Under more technology contents, when being also considered as the enforceable scope of the present invention.
Embodiment 1: a method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide
1, the preparation of culture material
Spot jade gill fungus Cultivar culture medium formula include following mass percent raw material: 30% sawdust, 33% corncob,
30% wheat bran, 5% corn flour and 2% gypsum.Wherein corncob is prewetted half an hour in advance, the water content of compost is
65%.Plastic bottle volume is 1100ml, and the amount per bottled material is between 720-750g, and then at 121 DEG C, pressure is under 0.1MPa
Sterilizing 2.5 hours.
2, the inoculation and culture of Cultivar culture medium
Cultivar culture medium is accessed into spot jade gill fungus the factorial production liquid spawn 10ml, is put into bacteria-producing room at 25 DEG C or so
Darkroom carries out dark culture, and the compost grown is carried out fruiting experiment.
3, different concentration of hydrogen peroxide experiments
By in step 2 grow into 85 days cultivar carry out mycelium stimulation, scratch old mycelia, then respectively add 2mM, 4mM,
The hydrogen peroxide and distilled water 15ml (comparative example) of 6mM and 8mM, transfers it in the fertility room in factory and carries out fruiting
Culture.
4, mushroom producing culture
Cultivation condition are as follows: humidity 85%-95%, 13-16 DEG C of temperature, illumination 300lx or so and carbon dioxide are in 3000ppm
Mushroom producing culture is carried out below.Through detecting, gas concentration lwevel 2891ppm.
5, harvesting and data statistics
When cultivar is cultivated about 22 days or so in biochemical cultivation case, cap is not yet unfolded, optimum period when spore not yet launches
Harvesting.Every bottle of fructification is weighed, average every bottle of yield (table 1) is calculated.As can be seen from Table 1, the peroxide of various concentration
Changing hydrogen has certain production-increasing function to spot jade gill fungus fruiting body yield, and when its concentration is 4mM, effect of increasing production is most significant, reaches
6.44%.
Influence of the different concentration of hydrogen peroxide of table 1 to spot jade gill fungus yield.
Embodiment 2: a method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide
1, the preparation of culture material
Spot jade gill fungus Cultivar culture medium formula include following mass percent raw material: 30% sawdust, 33% corncob,
30% wheat bran, 5% corn flour and 2% gypsum.Wherein corncob is prewetted half an hour in advance, the water content of compost is
65%.Plastic bottle volume is 1100ml, and the amount per bottled material is between 720-750g, and then at 121 DEG C, pressure is under 0.1MPa
Sterilizing 2.5 hours.
2, the inoculation and culture of Cultivar culture medium
Cultivar culture medium is accessed into spot jade gill fungus the factorial production liquid spawn 10ml, is put into bacteria-producing room at 25 DEG C or so
Darkroom carries out dark culture, and the compost grown is carried out fruiting experiment.
3, different cultural hypha time-triggered protocols
The cultivar for growing into 60 days, 80 days, 100 days and 120 days in step 2 is subjected to mycelium stimulation, scratches old mycelia, then
The hydrogen peroxide and distilled water 15ml (comparative example) for adding 4mM, are transferred into the fertility room in factory and carry out mushroom producing culture.
4, mushroom producing culture
Cultivation condition are as follows: humidity 85%-95%, 13-16 DEG C of temperature, illumination 300lx or so and carbon dioxide are in 3000ppm
Mushroom producing culture is carried out below.The gas concentration lwevel of the present embodiment is 2500ppm.
5, harvesting and data statistics
When cultivating about 22 days or so in the fertility room of cultivar in the factory, cap is not yet unfolded, when spore not yet launches
Optimum harvest.Every bottle of fructification is weighed, average every bottle of yield (table 2) is calculated.As can be seen from Table 2, mycelia is trained
Support the time be 80 days, add 4mM hydrogen peroxide fruiting body yield than the cultural hypha time be 100 days and 120 days yield all
Height, yield-increasing efficiency reach 8.19%, illustrate that external source addition hydrogen peroxide can shorten spot jade gill fungus cultivation period.
Influence of 2 hydrogen peroxide of table to different cultural hypha time spot jade gill fungus yield.
Embodiment 3: a method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide
Hydrogen peroxide improves and stablizes the method for spot jade gill fungus yield, it is characterised in that the normal strains of selection batch production are made
Time point for research object, hydrogen peroxide addition selects after mycelium stimulation, and the optium concentration for adding hydrogen peroxide is 4mM, addition
The best cultural hypha time of hydrogen peroxide is 80 days.
The method of hydrogen peroxide of the invention to spot jade gill fungus volume increase stable yields, including the following steps:
(1) industrial bottle carrying agent system
Spot jade gill fungus cultivation system is: plastic bottle volume is 1100ml, and cultivation wet feed is 720-750g/ bottles.
(2) research object
The bacterial strain of choice experiment is the normal production bacterial strain of batch production, uses the substance peroxidating with oxidisability
Hydrogen.
(2) mycelium stimulation and mushroom producing culture
Incubation time in step (1) is added after 80 days cultivar mycelium stimulations by the time point selection of hydrogen peroxide addition
Hydrogen peroxide.Mycelium stimulation is carried out using mechanical mycelium stimulation equipment, then adds hydrogen peroxide, hydrogen peroxide and the distillation that concentration is 4mM
Water 15ml transfers it to mushroom room and carries out mushroom producing culture.
Hydrogen peroxide, which both has, increases spot jade gill fungus fruiting body yield, it may have shortens the function in its period.
Present embodiment carries out in the factory of the factorial production spot jade gill fungus, the bacterial strain of selection be the factorial production just
Normal bacterial strain has primarily looked at the hydrogen peroxide (2mM, 4mM, 6mM and 8mM) of various concentration to spot jade gill fungus yield increasing effect, and discovery exists
Effect of increasing production is most significant when 4mM, reaches 6.44%；Then, peroxidating under different cultural hypha times (60-120 days) has been investigated
The yield increasing effect of hydrogen, discovery can improve spot jade gill fungus fruiting body yield significantly when incubation time is 80 days, reach 8.19%.
Embodiment 4: a method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide
A method of spot jade gill fungus fruiting body yield is improved using hydrogen peroxide, including the following steps:
Step 1: spot jade gill fungus the factorial production being inoculated in culture material with strain, then carries out dark culture；
Step 2: cultivating to after 120 days, carry out mycelium stimulation, and the hydrogen peroxide for being 2mM to the concentration of culture material addition 15ml；
Step 3: being then transferred into mushroom room and carry out mushroom producing culture.
Preferred technical solution are as follows: the composition of raw materials of the culture material includes the raw material of following mass percent: 32% wood
Bits, 34.5% corncob, 28% wheat bran, 4% corn flour and 1.5% gypsum.
Preferred embodiment are as follows: the half an hour of the corncob before the use is prewetted in advance；The culture material
Water content be 60-70%；The culture material is before inoculation, and using 121 DEG C, the parameter of pressure 0.1Mpa sterilizes 2.5 hours.
Preferred embodiment are as follows: every 720g culture material is inoculated with spot jade gill fungus the factorial production strain 10ml.
Preferred embodiment are as follows: the concentration of hydrogen peroxide is 4mM.
Preferred embodiment are as follows: the condition of mushroom producing culture are as follows: relative humidity 85%, temperature are 13 DEG C, illumination is
250lx, gas concentration lwevel 2800ppm.
Preferred embodiment are as follows: step 2: cultivating to after 85 days, carries out mycelium stimulation, and the concentration of 15ml is added to culture material
For the hydrogen peroxide of 4mM.
Preferred embodiment are as follows: temperature when dark culture is 27 DEG C.
As described above is only to be not intended to tool to explain the preferred embodiments of the invention to do any shape to the present invention
Limitation in formula should all wrap therefore all have any modification or change for making the related present invention under identical spirit
It includes in the scope that the invention is intended to protect.
Claims (8)
1. a kind of method for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: include the following steps:
Step 1: spot jade gill fungus the factorial production being inoculated in culture material with strain, then carries out dark culture；
Step 2: cultivating to after 60-120 days, carry out mycelium stimulation, and the hydrogen peroxide for being 2-8mM to the concentration of culture material addition 15ml；
Step 3: being then transferred into mushroom room and carry out mushroom producing culture.
2. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: institute
The composition of raw materials for stating culture material includes the raw material of following mass percent: 28-32% sawdust, 30-35% corncob, 28-30% bran
Skin, 4-6% corn flour and 1.5-2.5% gypsum.
3. the method according to claim 2 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: institute
The half an hour of corncob before the use is stated to be prewetted in advance；The water content of the culture material is 60-70%；The culture material
Before inoculation, using 121 DEG C, the parameter of pressure 0.1Mpa sterilizes 2.5 hours.
4. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: every
720-750g culture material is inoculated with spot jade gill fungus the factorial production strain 10ml.
5. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: double
The concentration of oxygen water is 4mM.
6. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: go out
Mushroom culture condition are as follows: relative humidity 85%-95%, temperature are 13-16 DEG C, illumination 250-350lx, carbon dioxide are less than or
Equal to 3000ppm.
7. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: step
Rapid 2: cultivating to after 80-85 days, carry out mycelium stimulation, and the hydrogen peroxide for being 4mM to the concentration of culture material addition 15ml.
8. the method according to claim 1 for improving spot jade gill fungus fruiting body yield using hydrogen peroxide, it is characterised in that: dark
Temperature when culture is 23-27 DEG C.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720625A (en) * | 2008-10-22 | 2010-06-09 | 河南农业大学 | Method for preventing mixed fungi from polluting edible fungi |
| CN106818209A (en) * | 2017-01-23 | 2017-06-13 | 上海市农业科学院 | A kind of application hydrogen rich water increases the method and its processing step in the degeneration-resistant border of spot jade gill fungus mycelia |
| CN107231946A (en) * | 2017-06-30 | 2017-10-10 | 上海市农业科学院 | A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot |
-
2019
- 2019-06-04 CN CN201910481592.6A patent/CN110063204A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720625A (en) * | 2008-10-22 | 2010-06-09 | 河南农业大学 | Method for preventing mixed fungi from polluting edible fungi |
| CN106818209A (en) * | 2017-01-23 | 2017-06-13 | 上海市农业科学院 | A kind of application hydrogen rich water increases the method and its processing step in the degeneration-resistant border of spot jade gill fungus mycelia |
| CN107231946A (en) * | 2017-06-30 | 2017-10-10 | 上海市农业科学院 | A kind of application hydrogen rich water raising and the method for the beautiful gill fungus fruiting body yield of stable spot |
Non-Patent Citations (1)
| Title |
|---|
| 高会东: "食用菌产前、产中、采后无公害生产技术", 《天津农学院学报》 * |
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