CN101919413B - Application of trichoderma in improving depression effect of medicament on rubber tree corynespora deciduous leaf pathogenic bacteria - Google Patents
Application of trichoderma in improving depression effect of medicament on rubber tree corynespora deciduous leaf pathogenic bacteria Download PDFInfo
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- CN101919413B CN101919413B CN 201010244896 CN201010244896A CN101919413B CN 101919413 B CN101919413 B CN 101919413B CN 201010244896 CN201010244896 CN 201010244896 CN 201010244896 A CN201010244896 A CN 201010244896A CN 101919413 B CN101919413 B CN 101919413B
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- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
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Abstract
The invention discloses application of trichoderma in improving depression effect of medicament on rubber tree corynespora deciduous leaf germs. The bacteriostatic effect of the medicament on the rubber tree corynespora deciduous leaf sopt pathogenic bacteria can be greatly improved by mixing the trichoderm and the midicament. Experiments prove that the application of the trichoderma can greatly improve the utilization ratio of the mdeicament and the bacteriostatic effect of the medicament on the rubber tree corynespora deciduous leaf sopt pathogenic bacteria, greatly lowers the cost for sterilization and disease prevention, and contributes to environmental protection.
Description
Technical field
The present invention relates to the application in the mould inhibition improving the medicament mould fallen leaves of excellent spore germ to bamboo grows of a kind of wood.
Background technology
Wood mould (Trichoderma) classification position is trichoderma (Trichoderma), is a genus of Eumycota, Deuteromycotina, Hyphomycetes, Moniliales.Morphological feature: mycelia is transparent have every, branch is luxuriant, conidiophore has giving birth to or the alternate branch, branch again on branch, branch top are stigma, ampuliform, Shu Sheng, to life, alternate or Dan Sheng, bear conidium by stigma, the spore head of a plurality of conidium adhesive aggregation globulates.The mould green mold that is commonly called as of wood.Belong to Eumycota, Deuteromycotina.wide at the occurring in nature distributed pole, pathogenicity to various edible mushrooms is strong, not only endanger the mycelial growth stage, also endanger fruit body of edible fungi, it is that in producing, generation is the most general, one of miscellaneous bacteria that harmfulness is the most serious, no matter the production of hybrid seeds or cultivate, no matter also raw material, grog, fermentation material, all can occur between bacteria developing period, even in the fruiting stage, generation is arranged also, this kind miscellaneous bacteria also can occur in the bacterial classification of indivedual kinds such as straw mushroom after completing a bacterium, and the temperature range that occurs is also more and more wider, the white fungus in many areas, mushroom, in recent years flat mushroom, destructive pollution once occured in the productions such as coprinus comatus, wood is mould is exactly one of main miscellaneous bacteria.The mould energy of wood production of cellulose enzyme, riboflavin biosynthesis is produced antibiotic, transforms steroid.On the mould fruit body that also often parasitizes some fungi of wood, be therefore the formidable opponent of cultured mushroom.
The mould feature of wood is to produce a large amount of dirty-green spores, has found the mould kind of many wood, as mould in many spores wood, koning trichoderma, Trichoderma viride etc.The Trichoderma silk is very thin, and white is transparent, and branch is arranged, and separates.The bacterium colony initial stage patch that is white in color, flocculence gradually becomes green after producing spore.The mould sporophore of wood is pinecone shape, and is long on the short lateral branch of mycelia, often makes 2-3 level branch.
The mould main existence of wood is on rotten wood, dry branches and fallen leaves, soil, fertilizer, plant residue and in air.Wood is mould to be distributed widely in various rotten wood, dry branches and fallen leaves, plant residue, soil and air, and natural world can find its family member everywhere.
At present, find the mould effect with the agriculture germ of inhibition of wood.
The innovation and creation content
The purpose of this invention is to provide the application in the mould inhibition improving the medicament mould fallen leaves of excellent spore germ to bamboo grows of a kind of wood.
The application that is applied as in the mould inhibition improving antimicrobial agent mould leaf spot pathogeny bacterium that falls to bamboo grows rod spore of wood provided by the invention.
Described antimicrobial agent is preferably the medicament that the mould leaf spot pathogeny bacterium that falls of bamboo grows rod spore is had sterilization or fungistatic effect.The mould leaf spot pathogeny bacterium that falls of described bamboo grows rod spore can be mould for melon rod spore, available from: Chinese agriculture microorganism fungus kind preservation administrative center (accc), preserving number is: 36068.
Described antimicrobial agent is preferably selected from one or more any mixing in the former medicine of mancozeb, mancozeb preparation, the former medicine of tpn, tpn preparation, the former medicine of pcnb, pcnb preparation, carbendazim active compound, carbendazim preparation, the former medicine of iprodione, iprodione preparation, the former medicine of Prochloraz and Prochloraz preparation.
Described mancozeb preparation is preferably mancozeb wettable powder, and described tpn preparation is the tpn wetting powder, and described pcnb preparation is the pcnb wetting powder, and described carbendazim preparation is carbendazol wettable powder; Described iprodione preparation is the iprodione wetting powder, and described Prochloraz preparation is prochloraz aqueous emulsion.
Described mancozeb preparation is 80% mancozeb wettable powder, described tpn preparation is 75% tpn wetting powder, described pcnb preparation is 20% pcnb wetting powder, and described carbendazim preparation is 50% carbendazol wettable powder; Described iprodione preparation is 50% iprodione wetting powder, and described Prochloraz preparation is 45% prochloraz aqueous emulsion; Described percentage composition is the quality percentage composition.
Described wood is mould for being preserved in the trichoderma strain of Chinese Academy of Agricultural Sciences microorganism fungus kind preservation administrative center, and preserving number is 30121.
Described wood is mould to improve antimicrobial agent be that wood is mould to the method for the inhibition of withered germ of water-melon and described antimicrobial agent is mixed use.
The present invention also provides a kind of bamboo grows rod spore the mould leaf spot pathogeny bacterium bacteriostatic agent that falls, by antibacterial medicament and wood mould composition; Described antimicrobial agent is selected from one or more any mixing in the former medicine of mancozeb, mancozeb preparation, the former medicine of tpn, tpn preparation, the former medicine of pcnb, pcnb preparation, carbendazim active compound, carbendazim preparation, the former medicine of iprodione, iprodione preparation, the former medicine of Prochloraz and Prochloraz preparation.
Described mancozeb preparation is preferably mancozeb wettable powder, described tpn preparation is preferably the tpn wetting powder, described pcnb preparation is preferably the pcnb wetting powder, and described carbendazim preparation is preferably carbendazol wettable powder; Described iprodione preparation is preferably the iprodione wetting powder, and described Prochloraz preparation is preferably prochloraz aqueous emulsion.
Described mancozeb preparation is preferably 80% mancozeb wettable powder, described tpn preparation is preferably 75% tpn wetting powder, described pcnb preparation is preferably 20% pcnb wetting powder, and described carbendazim preparation is preferably 50% carbendazol wettable powder; Described iprodione preparation is preferably 50% iprodione wetting powder, and described Prochloraz preparation is preferably 45% prochloraz aqueous emulsion; Described percentage composition is the quality percentage composition.
Experimental results show that application of the present invention can improve the control efficiency of medicament greatly; improve medicament to the fungistatic effect of the mould leaf spot pathogeny bacterium that falls of bamboo grows rod spore, can greatly reduce the cost of sterilizing disease-preventing, reduce the consumption of chemical agent; improve protection effect, environmental protection is made contributions.
The invention will be further described below in conjunction with the drawings and specific embodiments.
Description of drawings
Fig. 1 is tpn and the mould inhibition to HCc of wood (left side, contrast; In, tpn; The right side, tpn+wood is mould)
Fig. 2 is mancozeb and the mould inhibition to HCc of wood (left side, contrast; In, mancozeb; The right side, mancozeb+wood is mould)
Embodiment
Application in the mould inhibition improving medicament mould leaf spot pathogeny bacterium that falls to bamboo grows rod spore of embodiment 1, wood
1, use material
Bacterial strain: the mould leaf spot pathogeny bacterium that falls of bamboo grows rod spore (hereinafter to be referred as HCc: this bacterium name is called: melon rod spore is mould, available from: Chinese agriculture microorganism fungus kind preservation administrative center (accc), preserving number is: 36068; ); Trichoderma (Trichoderma.sp) is (V): Chinese Academy of Agricultural Sciences microorganism fungus kind preservation administrative center (accc), preserving number 30121.
Medicament: 1. 80% mancozeb wettable powder (trade name: red bacterium gram, Hebei Shuangji chemical limited company) 2. 75% tpn wetting powder (trade name: Da Kening, Syngenta Co.,Ltd) 3. 50% carbendazol wettable powder (trade name: carbendazim, Shanghai Nore chemical industry Co., Ltd).
2, the mould and medicament of wood mixes the inhibitory action of using the mould fallen leaves of bamboo grows rod spore germ
The mould fallen leaves of bamboo grows rod spore germ is inoculated on the medium that is coated with 1 described medicament in steps, then inoculate Trichoderma (Trichoderma.sp) (V), the inhibitory action of test agents to the mould fallen leaves of bamboo grows rod spore germ, use separately and without the medicament medium in contrast with the described medicament of step 1, concrete as table 1 and Fig. 1, shown in Figure 2, concrete grammar is as described below:
The excellent spore of growing 5 days on the PDA medium is mould with cultivating, beat along colony edge with the card punch of diameter 0.5cm and get the bacterium cake, bacterium faces down, be inoculated into and be coated with in steps 1 described medicament (with the certain density medicament of sterile water preparation, be added in 20 milliliters of PDA medium that dissolve, make the PDA that contains certain medicament final concentration dull and stereotyped.The final concentration of various medicaments is: 80% mancozeb-final concentration: 1 ‰; 75% tpn-final concentration 1.25 ‰; 50% carbendazim-final concentration: 0.83 ‰; Above-mentioned ‰ all represents mass content) the dull and stereotyped central authorities of diameter 9cm PDA, use same method, get the Trichoderma cake of 0.5cm diameter, be seeded in excellent spore mould around place with the equilateral triangle shape, each Trichoderma cake and pathogen cake be 3cm apart.Be placed on separately with pathogen on the PDA flat board that contains above-mentioned medicament and do not contain medicament and cultivate in contrast; Each processing arranges 3 repetitions, 28 ℃ of constant temperature culture, the diameter of pathogen in Trichoderma and disease fungus colony diameter and contrast during employing right-angled intersection method measurement face-off is cultivated, calculating bacteriostasis rate.Bacteriostasis rate (%)=[(contrast colony diameter-face-off colony diameter)/contrast colony diameter] * 100.Wherein, face-off is cultivated bacterium colony for containing medicament and inoculate the pathogen of cultivating on the flat board of Trichoderma or containing separately the pathogen of cultivating on the flat board of medicament above-mentioned.The contrast bacterium colony is for to be placed on separately with pathogen the pathogen of cultivating in the flat board that does not contain above-mentioned medicament.
PDA medium: potato 200 grams per liters, glucose 20 grams per liters, agar powder 20 grams per liters, distilled water.
Trichoderma viride and medicament see Table 1 to the inhibitory action of the mould fallen leaves of bamboo grows rod spore germ, as shown in Table 1, and in single dose is processed, 2. be that the inhibiting rate of tpn is for the highest with medicament, 57%, and effect the poorest be that 1. medicament is the inhibiting rate of mancozeb, be only 24.1%.In medicament and the mould symphyogenetic processing of wood, 2. medicament is that tpn and wooden mould acting in conjunction are the highest to the inhibiting rate of HCc, reaches 67%, and it is the poorest to be with medicament 1. that mancozeb adds the mould treatment effect of wood, is 29.9%; In medicament independent role and medicament and wooden mould the associating in two kinds of situations of use, unite the effect of use far above the effect of the independent use of medicament, reach extremely remarkable between two kinds of processing.Add wood mould and do not add between wooden processing when mould and reach extremely remarkable, particularly 2. 3. to add the inhibition of wooden mould raising particularly remarkable with medicament for medicament.Be chemical agent under the mould effect of wood, strengthened the inhibitory action to disease fungus, increased the control efficiency to pathogen.Fig. 1 is tpn and the mould inhibition to HCc of wood (left side, contrast; In, tpn; The right side, tpn+wood is mould) Fig. 2 is mancozeb and the mould inhibition to HCc of wood (left side, contrast; In, mancozeb; The right side, mancozeb+wood is mould)
Table 1 Trichoderma viride and medicament inhibitory action and the significance analysis to the mould fallen leaves of bamboo grows rod spore germ
Above-mentioned experiment showed, to mix with the medicament of the antibacterial or sterilization of chemistry with Trichoderma use, reach than the better effect of alone chemical agent, therefore can reduce the usage amount of chemical agent.And Trichoderma is to plant and environmentally friendly microorganism, and it is very low to cultivate cost.This method is by studying and take full advantage of the synergy of the mould leaf spot pathogeny bacterium that falls of biological control and chemical control bamboo grows rod spore, can greatly reduce chemical agent to the impact of environment, improve control efficiency, slow down disease to the resistant mutation of chemical agent, greatly reduce simultaneously the cost of control germ.
Claims (2)
1. be application during 30121 trichoderma strain is improving the inhibition of antimicrobial agent mould leaf spot pathogeny bacterium that falls to bamboo grows rod spore at the preserving number of Chinese Academy of Agricultural Sciences microorganism fungus kind preservation administrative center; Described antimicrobial agent is 75% tpn wetting powder; Described percentage composition is the quality percentage composition.
2. the mould leaf spot pathogeny bacterium bacteriostatic agent that falls of bamboo grows rod spore is by antimicrobial agent be that 30121 trichoderma strain forms at the preserving number of Chinese Academy of Agricultural Sciences microorganism fungus kind preservation administrative center; Described antimicrobial agent is 75% tpn wetting powder; Described percentage composition is the quality percentage composition.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010244896 CN101919413B (en) | 2009-12-25 | 2010-08-02 | Application of trichoderma in improving depression effect of medicament on rubber tree corynespora deciduous leaf pathogenic bacteria |
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| CN200910244034 | 2009-12-25 | ||
| CN200910244034.4 | 2009-12-25 | ||
| CN 201010244896 CN101919413B (en) | 2009-12-25 | 2010-08-02 | Application of trichoderma in improving depression effect of medicament on rubber tree corynespora deciduous leaf pathogenic bacteria |
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| CN 201010244896 Expired - Fee Related CN101919413B (en) | 2009-12-25 | 2010-08-02 | Application of trichoderma in improving depression effect of medicament on rubber tree corynespora deciduous leaf pathogenic bacteria |
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| CN102604869B (en) * | 2012-03-23 | 2013-05-29 | 南京农业大学 | Bio-control bacteria strain 1JN2 for preventing and treating bacterial fruit blotches of watermelons and application thereof |
| CN103131642B (en) * | 2013-03-18 | 2014-07-23 | 赵钦刚 | Trichoderma viride and culture method and application thereof |
| CN103255064B (en) * | 2013-04-08 | 2014-07-23 | 浙江省农业科学院 | Fungal agent for prevention and control of watermelon fusarium wilt and preparation method thereof |
| CN104012347A (en) * | 2014-06-19 | 2014-09-03 | 南京麦思德餐饮管理有限公司 | Method for preventing fusarium oxysporum schlecht |
| CN104982198B (en) * | 2015-06-26 | 2017-05-31 | 江苏省农业科学院 | The method and its special microorganism bacterial strain of prevention and control watermelon blight |
| CN105028488A (en) * | 2015-08-31 | 2015-11-11 | 红河学院 | Preparation method of trichoderma composite germicide |
| CN106591144B (en) * | 2016-12-01 | 2019-06-11 | 中国热带农业科学院橡胶研究所 | One plant of multifunctional wood trichoderma strain and its application |
| CN108689778B (en) * | 2018-06-22 | 2022-02-15 | 南京农业大学 | Trichoderma full-element bio-organic fertilizer special for pressed-sand watermelon and preparation method and application thereof |
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Non-Patent Citations (6)
| Title |
|---|
| 吴利民,等.哈茨木霉TR-12对多主棒孢霉菌的拮抗作用.《湖北农业科学》.2006,第45卷(第6期),第749-750页. |
| 吴石平,等.木霉菌与三唑酮配合对西瓜生长的影响和对枯萎病的防效.《西南农业学报》.2002,第15卷(第2期),第65-68页. |
| 哈茨木霉TR-12对多主棒孢霉菌的拮抗作用;吴利民,等;《湖北农业科学》;20061130;第45卷(第6期);第749-750页 * |
| 木霉菌与三唑酮配合对西瓜生长的影响和对枯萎病的防效;吴石平,等;《西南农业学报》;20021231;第15卷(第2期);第65-68页 * |
| 橡胶树棒孢霉落叶斑病菌的室内药剂筛选;谢昌平,等;《热带农业科学》;20071231;第27卷(第6期);第5-7,39页 * |
| 谢昌平,等.橡胶树棒孢霉落叶斑病菌的室内药剂筛选.《热带农业科学》.2007,第27卷(第6期),第5-7,39页. |
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| CN101919414A (en) | 2010-12-22 |
| CN101919413A (en) | 2010-12-22 |
| CN101919414B (en) | 2013-05-08 |
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