CN107223762A - A kind of napier grass biological feedstuff and its production and use - Google Patents
A kind of napier grass biological feedstuff and its production and use Download PDFInfo
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
The present invention relates to feed and its processing technique field, more particularly to a kind of napier grass biological feedstuff and its production and use, napier grass biological feedstuff of the invention is by napier grass, auxiliary material and probiotic using weight ratio as 20 30:10‑15:13 mixed fermentations are made, mainly pass through probiotics and cellulose degrading enzyme fermentation process napier grass, napier grass, which is fermented, after-tack and degrades the fiber in napier grass, improve the palatability of napier grass, extend the pot-life of napier grass, napier grass biological feedstuff is produced using the present processes, the holding time of napier grass can be made up to more than 2 years, simultaneously, the napier grass biological feedstuff of the present invention is the culture medium for being adapted to beneficial microbe growth, animal edible in vivo after, it is numerous that beneficial microbe in animal body has obtained expansion after fermentation, it can effectively improve animal digestion performance, to reduction animal wastes stink, improve barn environment to play a very important role.
Description
【Technical field】
The present invention relates to feed and its processing technique field, more particularly to a kind of napier grass biological feedstuff and preparation method thereof and
Purposes.
【Background technology】
Napier grass, is one of yield highest herbage in herbage, is the main herbage variety in current southern area, napier grass is because of it
Containing higher cellulose, while low percentages of protein etc., its nutritive value is relatively poor, fiber content is higher, in reality
The application that can only be largely used when plant-eating animal cultivates in production and be used as omnivorous animal (cultivation of such as pig) feed is seldom,
And feeding effect is general, napier grass, using less, is to be fed using fresh mostly during napier grass feeding, often in other animal feeds such as pig farm
It is required for harvesting, processed, and not only process is cumbersome, is also inconvenient for the popularization of grain-saving type livestock-raising technology, is supported in the routine of pig
It is the feedstuff for using wheat bran based on supplementary fibre mostly during growing, due to itself physics and chemical characteristic, wheat bran
Easily gone mouldy in storage, a kind of necessary feed of exploitation substitutes wheat bran as the fiber source in swine rationses, and in pig
In breeding process, in order to the pig that shortens the stage of fattening growth time, for nursing period, gravidic pig supplement nutrient there is provided safety,
Efficient feed, requires more accurate to the protein in feed, amino acid content.
【The content of the invention】
In view of the above, it is necessary to provide a kind of napier grass biological feedstuff and its production and use, can substitute completely
Wheat bran is used on pig farm, and is had napier grass acid perfume, is easy to preservation, digestibility height, the preferable biological feedstuff of palatability, moreover it is possible to prolonged
The pot-life of long napier grass.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, the weight ratio of the napier grass, auxiliary material and probiotic
For 20-30:10-15:1-3；
The auxiliary material includes the composition of following parts by weight：40 parts -60 parts of corn bran, 10 parts -20 parts of molasses and at least 3
Plant the composition of following parts by weight：0-10 parts of flaccid knotweed herb extract, 0-10 parts of Semen phaseoli radiati extract, 0-10 parts of skimmed milk power and
0-10 parts of aloe extract；
The probiotic includes the composition of following parts by weight：25 parts -40 parts of cellulose degrading enzyme and at least three kinds of following weights
Measure the composition of part：0-10 parts of lactic acid bacteria, 0-10 parts of saccharomycete, 0-10 parts of bacillus subtilis, 0-10 parts of green juice hair
Zymotic fluid.
Further, the extracting method of the flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 10-15
The percentage by volume of times quality be put into after 50% (v/v) -60% (v/v) alcohol solution dipping, immersion 15min-20min it is super
Carried out in sound extractor after ultrasonic extraction, ultrasonic extraction 40min-50min, filtered, take filtrate to be concentrated, drying is obtained
Flaccid knotweed herb extract；The power of the ultrasonic extraction is 80w-90w, and temperature is 50 DEG C -60 DEG C, and pH is 6-7.
Further, the extracting method of the Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content
For 30%-40%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in ultrasonic extraction
Carry out after ultrasonic extraction, ultrasonic extraction 50min-60min, filtered in device, take filtrate to add the volume basis of 2-5 times of quality
Number is soaked for 60% (v/v) -70% (v/v) ethanol solution, after immersion 12h-14h, is filtered, and takes filtrate to carry out dense
Contracting, drying obtains Semen phaseoli radiati extract；The power of the ultrasonic extraction is 50w-60w, and temperature is 45 DEG C -55 DEG C, and pH is 7-8.
Further, the extracting method of the aloe extract is：It is put into mortar and is ground after aloe is removed the peel, so
Add afterwards equal quality than percentage by volume soaked for 70% (v/v) -80% (v/v) ethanol solution, immersion 24h-
After 26h, it is stirred, filters, takes filtrate to be concentrated, dries and obtain aloe extract.
Present invention also offers a kind of method for preparing napier grass biological feedstuff, methods described comprises the following steps：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and sent out
Ferment feed；The oxygen content of the aerobic fermentation is 5%-10%, and temperature is 22 DEG C -26 DEG C, and humidity is 6%-10%, and the time is
24h-26h；The fermentation temperature of the anaerobic fermentation is 22 DEG C -26 DEG C, and humidity is 6%-10%, and the time is 15d-20d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 40 DEG C -50 DEG C, constant temperature drying, until
Moisture content is that 10%-20% obtains napier grass biological feedstuff.
Present invention also offers the purposes of napier grass biological feedstuff, the napier grass biological feedstuff is applied to the pig, pregnant of fattening period
It is pregnent the sow of phase or the sow of nursing period.
The present invention has the advantages that：
1st, the present invention is by probiotics and cellulose degrading enzyme fermentation process napier grass, napier grass fermentation after-tack and degrade as
Fiber in grass, improves the palatability of napier grass, extends the pot-life of napier grass, and it is biological to produce napier grass using the present processes
Feed, can make the holding time of napier grass up to more than 2 years, meanwhile, napier grass biological feedstuff of the invention is to be adapted to beneficial microbe
The culture medium of growth, in animal edible to after in vivo, it is numerous that the beneficial microbe in animal body has obtained expansion after fermentation, can be effective
Improve animal digestion performance, reduction animal wastes stink, improvement barn environment are played a very important role.
2nd, main composition is containing abundant crude fibre, still in napier grass, napier grass in the napier grass biological feedstuff of the application
Its pot-life is low, nutritional ingredient is single, and making feed to pig can cause pig to digest, the defect such as nutritional ingredient deficiency, application
People find to add in napier grass after research molasses, corn bran, probiotics etc. together fermentation energy effectively soften it is thick in napier grass
Fiber, in this formula, molasses provide enough sugars as power source for fermentation process, and corn bran provides foot for fermentation process
Enough protein, and the probiotics that fermentation process is used：Cellulose degrading enzyme can effectively degrade the cellulose in napier grass, play soft
The effect of chemical fibre dimension, probiotics：Lactic acid bacteria, saccharomycete, bacillus subtilis and fermented green juice can not only fully ferment napier grass,
Can also be enriched with feed rapidly, indispensable effect is played to improving chitling road, flaccid knotweed herb extract, Semen phaseoli radiati extract,
Skimmed milk power and aloe extract can effectively suppress varied bacteria growing, improve the growth ability of beneficial bacterium.
【Embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated otherwise, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
A kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, wherein, state the weight of napier grass, auxiliary material and probiotic
Than for 20:10:1；
The present embodiment auxiliary material, probiotic include parts by weight ingredient as described in Table 1：
Table 1
In the present embodiment, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 10 times of quality
Percentage by volume be 50% (v/v) alcohol solution dipping, immersion 15min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 40min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 80w, temperature be 50 DEG C, pH is 6.
In the present embodiment, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
30%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 50min, filtered, the percentage by volume for taking filtrate to add 2 times of quality is 60% (v/v)
Ethanol solution soaked, immersion 12h after, filtered, take filtrate to be concentrated, dry obtain Semen phaseoli radiati extract；It is described
The power of ultrasonic extraction is 50w, and temperature is 45 DEG C, and pH is 7.
The preparation method of above-mentioned napier grass biological feedstuff comprises the following steps：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and sent out
Ferment feed；The oxygen content of the aerobic fermentation is 5%, and temperature is 22 DEG C, and humidity is 6%, and the time is 24h；The anaerobic fermentation
Fermentation temperature be 22 DEG C, humidity is 6%, and the time is 15d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 40 DEG C, constant temperature drying, until aqueous
Rate obtains napier grass biological feedstuff for 10%.
Embodiment 2：
A kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, wherein, state the weight of napier grass, auxiliary material and probiotic
Than for 30:15:3；
The present embodiment auxiliary material, probiotic include parts by weight ingredient as described in Table 2：
Table 2
In the present embodiment, the extracting method of aloe extract is：It is put into mortar and is ground after aloe is removed the peel, then
Add equal quality than percentage by volume soaked for 70% (v/v) ethanol solution, soak after 24h, be stirred, mistake
Filter, takes filtrate to be concentrated, dries and obtain aloe extract.
In the present embodiment, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
40%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 60min, filtered, the percentage by volume for taking filtrate to add 5 times of quality is 70% (v/v)
Ethanol solution soaked, immersion 14h after, filtered, take filtrate to be concentrated, dry obtain Semen phaseoli radiati extract；It is described
The power of ultrasonic extraction is 60w, and temperature is 55 DEG C, and pH is 8.
The preparation method of above-mentioned napier grass biological feedstuff comprises the following steps：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and sent out
Ferment feed；The oxygen content of the aerobic fermentation is 10%, and temperature is 26 DEG C, and humidity is 10%, and the time is 26h；The anaerobism hair
The fermentation temperature of ferment is 26 DEG C, and humidity is 10%, and the time is 17d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 50 DEG C, constant temperature drying, until aqueous
Rate obtains napier grass biological feedstuff for 20%.
Embodiment 3：
A kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, wherein, state the weight of napier grass, auxiliary material and probiotic
Than for 15:12:2；
The present embodiment auxiliary material, probiotic include parts by weight ingredient as described in Table 3：
Table 3
In the present embodiment, the extracting method of aloe extract is：It is put into mortar and is ground after aloe is removed the peel, then
Add equal quality than percentage by volume soaked for 80% (v/v) ethanol solution, soak after 26h, be stirred, mistake
Filter, takes filtrate to be concentrated, dries and obtain aloe extract.
In the present embodiment, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
35%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 55min, filtered, the percentage by volume for taking filtrate to add 3 times of quality is 65% (v/v)
Ethanol solution soaked, immersion 13h after, filtered, take filtrate to be concentrated, dry obtain Semen phaseoli radiati extract；It is described
The power of ultrasonic extraction is 55w, and temperature is 50 DEG C, and pH is 7.5.
In the present embodiment, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 12 times of quality
Percentage by volume be 55% (v/v) alcohol solution dipping, immersion 17min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 45min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 85w, temperature be 55 DEG C, pH is 6.5.
The preparation method of above-mentioned napier grass biological feedstuff comprises the following steps：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and sent out
Ferment feed；The oxygen content of the aerobic fermentation is 7%, and temperature is 23 DEG C, and humidity is 7%, and the time is 25h；The anaerobic fermentation
Fermentation temperature be 23 DEG C, humidity is 7%, and the time is 16d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 45 DEG C, constant temperature drying, until aqueous
Rate is that 10%-20% obtains napier grass biological feedstuff.
Embodiment 4：
A kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, wherein, state the weight of napier grass, auxiliary material and probiotic
Than for 30:13:1；
The present embodiment auxiliary material, probiotic include parts by weight ingredient as described in Table 4：
Table 4
In the present embodiment, the extracting method of aloe extract is：It is put into mortar and is ground after aloe is removed the peel, then
Add equal quality than percentage by volume soaked for 73% (v/v) ethanol solution, soak after 24.5h, be stirred,
Filtering, takes filtrate to be concentrated, dries and obtain aloe extract.
In the present embodiment, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
33%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 53min, filtered, the percentage by volume for taking filtrate to add 4 times of quality is 63% (v/v)
Ethanol solution soaked, immersion 13h after, filtered, take filtrate to be concentrated, dry obtain Semen phaseoli radiati extract；It is described
The power of ultrasonic extraction is 56w, and temperature is 51 DEG C, and pH is 7.6.
In the present embodiment, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 13 times of quality
Percentage by volume be 57% (v/v) alcohol solution dipping, immersion 17min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 48min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 83w, temperature be 54 DEG C, pH is 6.6.
The preparation method of above-mentioned napier grass biological feedstuff comprises the following steps：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and sent out
Ferment feed；The oxygen content of the aerobic fermentation is 8%, and temperature is 24 DEG C, and humidity is 8%, and the time is 25h；The anaerobic fermentation
Fermentation temperature be 23 DEG C, humidity is 8%, and the time is 20d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 49 DEG C, constant temperature drying, until aqueous
Rate obtains napier grass biological feedstuff for 17%.
Embodiment 5：
A kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, wherein, state the weight of napier grass, auxiliary material and probiotic
Than for 30:13:2；
The present embodiment auxiliary material, probiotic include parts by weight ingredient as described in Table 5：
Table 5
In the present embodiment, the extracting method of aloe extract is：It is put into mortar and is ground after aloe is removed the peel, then
Add equal quality than percentage by volume soaked for 77% (v/v) ethanol solution, soak after 25h, be stirred, mistake
Filter, takes filtrate to be concentrated, dries and obtain aloe extract.
In the present embodiment, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
36%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 57min, filtered, the percentage by volume for taking filtrate to add 2-5 times of quality is 68% (v/
V) ethanol solution is soaked, and after immersion 13h, is filtered, takes filtrate to be concentrated, drying obtains Semen phaseoli radiati extract；Institute
The power for stating ultrasonic extraction is 56w, and temperature is 53 DEG C, and pH is 8.
In the present embodiment, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 14 times of quality
Percentage by volume be 58% (v/v) alcohol solution dipping, immersion 19min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 48min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 87w, temperature be 56 DEG C, pH is 7.
The preparation method of above-mentioned napier grass biological feedstuff comprises the following steps：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and sent out
Ferment feed；The oxygen content of the aerobic fermentation is 7%, and temperature is 25 DEG C, and humidity is 10%, and the time is 26h；The anaerobic fermentation
Fermentation temperature be 25 DEG C, humidity is 10%, and the time is 20d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 50 DEG C, constant temperature drying, until aqueous
Rate obtains napier grass biological feedstuff for 18%.
Control group 1：
Other parameters and embodiment 5 are completely the same, and only the composition of auxiliary material probiotic is different, auxiliary material, prebiotic component such as table 6
It is shown：
Table 6
In this control group, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 14 times of quality
Percentage by volume be 58% (v/v) alcohol solution dipping, immersion 19min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 48min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 87w, temperature be 56 DEG C, pH is 7.
In this control group, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
36%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 57min, filtered, the percentage by volume for taking filtrate to add 2-5 times of quality is 68% (v/
V) ethanol solution is soaked, and after immersion 13h, is filtered, takes filtrate to be concentrated, drying obtains Semen phaseoli radiati extract；Institute
The power for stating ultrasonic extraction is 56w, and temperature is 53 DEG C, and pH is 8.
Control group 2：
Other parameters and embodiment 5 are completely the same, and only the composition of auxiliary material probiotic is different, auxiliary material, prebiotic component such as table 7
It is shown：
Table 7
In this control group, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 14 times of quality
Percentage by volume be 58% (v/v) alcohol solution dipping, immersion 19min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 48min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 87w, temperature be 56 DEG C, pH is 7.
In this control group, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
36%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 57min, filtered, the percentage by volume for taking filtrate to add 2-5 times of quality is 68% (v/
V) ethanol solution is soaked, and after immersion 13h, is filtered, takes filtrate to be concentrated, drying obtains Semen phaseoli radiati extract；Institute
The power for stating ultrasonic extraction is 56w, and temperature is 53 DEG C, and pH is 8.
Control group 3：
Other parameters and embodiment 5 are completely the same, and only the composition of auxiliary material probiotic is different, auxiliary material, prebiotic component such as table 8
It is shown：
Table 8
Control group 4：
Other parameters and embodiment 5 are completely the same, and only the composition of auxiliary material probiotic is different, auxiliary material, prebiotic component such as table 9
It is shown：：
Table 9
In this control group, the extracting method of flaccid knotweed herb extract is：Take the whole strain of fresh flaccid knotweed herb to smash to pieces, add 14 times of quality
Percentage by volume be 58% (v/v) alcohol solution dipping, immersion 19min after be put into supersonic extractors carry out ultrasound carry
Take, after ultrasonic extraction 48min, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The ultrasonic extraction
Power be 87w, temperature be 56 DEG C, pH is 7.
In this control group, the extracting method of Semen phaseoli radiati extract is：Fresh mung bean is taken, is soaked in water until mung bean moisture content is
36%, then add with mung bean equal quality than water mung bean broken into juice with juicer, be then placed in supersonic extractors
After row ultrasonic extraction, ultrasonic extraction 57min, filtered, the percentage by volume for taking filtrate to add 2-5 times of quality is 68% (v/
V) ethanol solution is soaked, and after immersion 13h, is filtered, takes filtrate to be concentrated, drying obtains Semen phaseoli radiati extract；Institute
The power for stating ultrasonic extraction is 56w, and temperature is 53 DEG C, and pH is 8.
Control group 5：
Other parameters and embodiment 5 are completely the same, and only the composition of auxiliary material probiotic is different, auxiliary material, prebiotic component such as table
Shown in 10：
Table 10
Testing experiment：
Determine the soluble-carbohydrate, protein, neutrality of embodiment 1-5 and control group 1-5 napier grass biological feedstuff
Fiber, acid detergent fiber, hemicellulose level are washed, it is specific as shown in table 11：
Table 11
Same column shoulder mark in upper table, different lowercase letter indication differences are notable.
As seen from the above table, embodiment 1-5 water-soluble carbohydrate contents, protein content are higher than control group 1-5, say
It is bright, it can effectively improve the content of soluble-carbohydrate and protein using the method production napier grass biological feedstuff of the present invention；
Embodiment 1-5 neutral detergent fiber, acid detergent fiber, hemicellulose is significantly lower than control group 1-5, illustrates, uses this hair
Bright method production napier grass biological feedstuff can effectively reduce content of cellulose.
Feeding experiment：
1st, growing and fattening pigs experiment (20kg-110kg)
The stage of fattening refers to that piglet grows to 110kg from 20kg；
Carried out at the test site of this institute, the consistent miscellaneous commodity of ternary of experiment selection age, body weight are divided into 7 groups, every group 100
Head, experimental stage is to be delivered for sale after 20kg child care terminates to 110kg.
The feed formula of test group 1：Corn 62%, dregs of beans 20%, the fermentation napier grass biological feedstuff 16% of embodiment 5, pig
With premix 2%, take off mould dose 1kg/ tons；
The feed formula of test group 2：Corn 62%, dregs of beans 20%, the fermentation napier grass biological feedstuff 16% of control group 1, pig
With premix 2%, take off mould dose 1kg/ tons；
The feed formula of test group 3：Corn 62%, dregs of beans 20%, the fermentation napier grass biological feedstuff 16% of control group 2, pig
With premix 2%, take off mould dose 1kg/ tons；
The feed formula of test group 4：Corn 62%, dregs of beans 20%, the fermentation napier grass biological feedstuff 16% of control group 3, pig
With premix 2%, take off mould dose 1kg/ tons；
The feed formula of test group 5：Corn 62%, dregs of beans 20%, the fermentation napier grass biological feedstuff 16% of control group 4, pig
With premix 2%, take off mould dose 1kg/ tons；
The feed formula of test group 6：Corn 62%, dregs of beans 20%, the fermentation napier grass biological feedstuff 16% of control group 5, pig
With premix 2%, take off mould dose 1kg/ tons；
The feed formula of control group：Corn 62%, dregs of beans 20%, wheat bran 16%, pig premix 2%.
Feeding is carried out to the pig of the stage of fattening by the feed formula of test group 1-6 and control group, other management methods are consistent, go out
It is as shown in table 12 by statistical result behind column：
Table 12
| Index | 120kg marketing ages * | Average daily gain | Feedstuff-meat ratio | Feed cost is per ton |
| Test group 1 | 168.2 | 855.51g | 2.64 | 2080 |
| Test group 2 | 174.25 | 768.32g | 2.86 | 2351 |
| Test group 3 | 173.31 | 768.16g | 2.86 | 2361 |
| Test group 4 | 176.31 | 759.21g | 2.84 | 2380 |
| Test group 5 | 177.15 | 764.26g | 2.94 | 2560 |
| Test group 6 | 179.36 | 764.31g | 2.87 | 2460 |
| Control group | 179.41 | 773.20g | 2.81 | 2340 |
(" * " is correction 120kg ages in days in form)
As seen from the above table, test group 1 (substituting wheat bran using the fermentation napier grass biological feedstuff of embodiment 5), can shorten pig
Marketing time, improve average daily gain, reduction feedstuff-meat ratio, effectively reduce cost, and test group 2-6 uses control group 1-
5 napier grass biological feedstuff, basically identical with the marketing time of control group, average daily gain, feedstuff-meat ratio, feed cost, explanation makes
Napier grass biological feedstuff is produced with control group 1-5 method, beneficial effect is not protruded.
2nd, sow is tested
The kind pig farm of selection one livestock on hand, 600 sows is tested, and there are perfect Breeding notes on the pig farm, by sow point
Into 6 groups in the gestational period, the nursing period difference following feed formula of feeding, after the test recipe using 1 year, before contrast test
The breeding statistics of 1 year, as a result such as table 13：
Experiment periods feed formula：
(1) sow forage for gestation period formula is as follows：
Test group 1：Corn 60%, dregs of beans 18%, the napier grass biological feedstuff 20% of embodiment 5, pig premix 2%；
Test group 2：Corn 60%, dregs of beans 18%, the napier grass biological feedstuff 20% of control group 1, pig premix 2%；
Test group 3：Corn 60%, dregs of beans 18%, the napier grass biological feedstuff 20% of control group 2, pig premix 2%；
Test group 4：Corn 60%, dregs of beans 18%, the napier grass biological feedstuff 20% of control group 3, pig premix 2%；
Test group 5：Corn 60%, dregs of beans 18%, the napier grass biological feedstuff 20% of control group 4, pig premix 2%；
Test group 6：Corn 60%, dregs of beans 18%, the napier grass biological feedstuff 20% of control group 5, pig premix 2%；
(2) lactation sow forage formula is as follows：
Test group 1：Corn 64%, dregs of beans 23%, fatty powder 1%, the napier grass biological feedstuff 10% of embodiment 5, pig premix
Material 2%；
Test group 2：Corn 64%, dregs of beans 23%, fatty powder 1%, the napier grass biological feedstuff 10% of control group 1, pig premix
Material 2%；
Test group 3：Corn 64%, dregs of beans 23%, fatty powder 1%, the napier grass biological feedstuff 10% of control group 2, pig premix
Material 2%；
Test group 4：Corn 64%, dregs of beans 23%, fatty powder 1%, the napier grass biological feedstuff 10% of control group 3, pig premix
Material 2%；
Test group 5：Corn 64%, dregs of beans 23%, fatty powder 1%, the napier grass biological feedstuff 10% of control group 4, pig premix
Material 2%；
Test group 6：Corn 64%, dregs of beans 23%, fatty powder 1%, the napier grass biological feedstuff 10% of control group 5, pig premix
Material 2%；
Compare group of formula (before experiment periods 1 year)：
In-pig is formulated：Corn 59%, dregs of beans 18%, wheat bran 20%, pig premix 2%, de- mould dose 1kg/ tons.
Milking sow is formulated：It is corn 63%, dregs of beans 23%, fatty powder 1%, wheat bran 10%, pig premix 2%, de- mould
1kg/ tons of agent.
Table 13
| Index | Year number born alive (head) | The survival rate that weans (%) | Average weight of weaning litter (kg) |
| Test group 1 | 28.9 | 98.2a | 82.69 |
| Test group 2 | 25.3 | 96.3a | 74.36 |
| Test group 3 | 26.4 | 95.7a | 75.16 |
| Test group 4 | 25.9 | 96.6b | 74.36 |
| Test group 5 | 26.1 | 94.3ab | 74.66 |
| Test group 6 | 26.7 | 95.2b | 75.36 |
| Control group | 26.4 | 95.4b | 73.46 |
Same column shoulder mark, different lowercase letter indication differences are notable.
Found by contrasting, test group 1 year number born alive, wean survival rate, average weight of weaning litter be significantly greater than experiment
Group 2-6 and control group, illustrate, sow is fed using napier grass fermenting organism feed produced by the invention, can be with the year of raising sow
Number born alive, improves the wean survival rate of piggy, increases the average weight of weaning litter of piggy.
In summary, using napier grass biological feedstuff produced by the invention, wheat bran can be substituted completely and be used on pig farm, can be effective
The protein content in feed is improved, fiber content is reduced, is more suitable for pig, even nursing period, gravidic sow eats, can had
Effect improves feed quality, reduces feed cost.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (6)
1. a kind of napier grass biological feedstuff, including napier grass, auxiliary material and probiotic, it is characterised in that the napier grass, auxiliary material and probiotic
Weight ratio be, 20-30:10-15:1-3；
The auxiliary material includes the composition of following parts by weight：40 parts -60 parts of corn bran, 10 parts -20 parts of molasses and it is at least three kinds of such as
The composition of lower parts by weight：0-10 parts of flaccid knotweed herb extract, 0-10 parts of Semen phaseoli radiati extract, 0-10 parts of skimmed milk power and 0-10
The aloe extract of part；
The probiotic includes the composition of following parts by weight：25 parts -40 parts of cellulose degrading enzyme and at least three kinds of following parts by weight
Composition：0-10 parts of lactic acid bacteria, 0-10 parts of saccharomycete, 0-10 parts of bacillus subtilis, 0-10 parts of fermented green juice.
2. a kind of napier grass biological feedstuff according to claim 1, it is characterised in that the extracting method of the flaccid knotweed herb extract
For：The whole strain of fresh flaccid knotweed herb is taken to smash to pieces, the percentage by volume for adding 10-15 times of quality is 50% (v/v) -60% (v/v) second
Alcoholic solution soaks, and is put into after immersion 15min-20min in supersonic extractors and carries out ultrasonic extraction, ultrasonic extraction 40min-50min
Afterwards, filtered, take filtrate to be concentrated, drying obtains flaccid knotweed herb extract；The power of the ultrasonic extraction is 80w-90w,
Temperature is 50 DEG C -60 DEG C, and pH is 6-7.
3. a kind of napier grass biological feedstuff according to claim 1, it is characterised in that the extracting method of the Semen phaseoli radiati extract
For：Take fresh mung bean, be soaked in water until mung bean moisture content be 30%-40%, then add with mung bean equal quality than water
Mung bean is broken into juice with juicer, is then placed in supersonic extractors and carries out ultrasonic extraction, after ultrasonic extraction 50min-60min,
Filtered, take filtrate to add the percentage by volume of 2-5 times of quality and soaked for 60% (v/v) -70% (v/v) ethanol solution
After bubble, immersion 12h-14h, filtered, take filtrate to be concentrated, drying obtains Semen phaseoli radiati extract；The work(of the ultrasonic extraction
Rate is 50w-60w, and temperature is 45 DEG C -55 DEG C, and pH is 7-8.
4. a kind of napier grass biological feedstuff according to claim 1, it is characterised in that the extracting method of the aloe extract
For：Be put into mortar and be ground after aloe is removed the peel, then add equal quality than percentage by volume for 70% (v/v)-
80% (v/v) ethanol solution is soaked, and after immersion 24h-26h, is stirred, is filtered, take filtrate to be concentrated, dried
To aloe extract.
5. a kind of method of the napier grass biological feedstuff prepared described in claim 1-4, it is characterised in that methods described includes as follows
Step：
(1) each composition is weighed by the weight ratio, napier grass is smashed, auxiliary material is added after sub-wire and probiotic is well mixed；
(2) mixture of step (1) is filled into advanced aerobe fermentation of acting charitably in fermenting cellar, then carries out anaerobic fermentation and obtain fermentation feeding
Material；The oxygen content of the aerobic fermentation is 5%-10%, and temperature is 22 DEG C -26 DEG C, and humidity is 6%-10%, and the time is 24h-
26h；The fermentation temperature of the anaerobic fermentation is 22 DEG C -26 DEG C, and humidity is 6%-10%, and the time is 15d-20d；
(3) after step (2) fermentation ends, fermented feed is taken out, at a temperature of 40 DEG C -50 DEG C, constant temperature drying, until aqueous
Rate is that 10%-20% obtains napier grass biological feedstuff.
6. the purposes of the napier grass biological feedstuff according to claim 1-4, it is characterised in that the napier grass biological feedstuff application
Pig, gravidic sow or the sow of nursing period in fattening period.
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| CN107821751A (en) * | 2017-11-16 | 2018-03-23 | 广西壮族自治区畜牧研究所 | A kind of kudzu feed and its preparation method and application |
| CN107865187A (en) * | 2017-11-16 | 2018-04-03 | 广西壮族自治区畜牧研究所 | A kind of purple napier grass feed and its preparation method and application |
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| CN107821751A (en) * | 2017-11-16 | 2018-03-23 | 广西壮族自治区畜牧研究所 | A kind of kudzu feed and its preparation method and application |
| CN107865187A (en) * | 2017-11-16 | 2018-04-03 | 广西壮族自治区畜牧研究所 | A kind of purple napier grass feed and its preparation method and application |
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| CN108271932A (en) * | 2018-01-31 | 2018-07-13 | 广西壮族自治区畜牧研究所 | It is a kind of using purple napier grass and roundleaf telegraph plant as the ox ecological fermentation feed of substrate |
| CN108185187A (en) * | 2018-02-14 | 2018-06-22 | 广东大广生物科技有限公司 | The sweet tea napier grass fermentation material and preparation method of raising farrowing sow production performance and application |
| CN109315614A (en) * | 2018-10-09 | 2019-02-12 | 四川华方生态养殖有限公司 | One boar cultivates health-care feed and preparation method thereof |
| CN109699831A (en) * | 2019-03-06 | 2019-05-03 | 广西壮族自治区畜牧研究所 | A kind of mango slag biological feedstuff and its preparation method and application |
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