CN107212236A - 一种复合生物防腐剂及其制备方法 - Google Patents
一种复合生物防腐剂及其制备方法 Download PDFInfo
- Publication number
- CN107212236A CN107212236A CN201710493908.4A CN201710493908A CN107212236A CN 107212236 A CN107212236 A CN 107212236A CN 201710493908 A CN201710493908 A CN 201710493908A CN 107212236 A CN107212236 A CN 107212236A
- Authority
- CN
- China
- Prior art keywords
- parts
- powder
- composite biological
- biological preservative
- glycine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003755 preservative agent Substances 0.000 title claims abstract description 33
- 230000002335 preservative effect Effects 0.000 title claims abstract description 30
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 65
- -1 N benzyloxycarbonyl group D aspartic acids Chemical class 0.000 claims abstract description 43
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 41
- 150000007524 organic acids Chemical class 0.000 claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 claims abstract description 21
- REPVLJRCJUVQFA-UHFFFAOYSA-N (-)-isopinocampheol Natural products C1C(O)C(C)C2C(C)(C)C1C2 REPVLJRCJUVQFA-UHFFFAOYSA-N 0.000 claims abstract description 21
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims abstract description 21
- 108010053775 Nisin Proteins 0.000 claims abstract description 21
- 229940116229 borneol Drugs 0.000 claims abstract description 21
- CKDOCTFBFTVPSN-UHFFFAOYSA-N borneol Natural products C1CC2(C)C(C)CC1C2(C)C CKDOCTFBFTVPSN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000012153 distilled water Substances 0.000 claims abstract description 21
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000004309 nisin Substances 0.000 claims abstract description 21
- 235000010297 nisin Nutrition 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 241000605422 Asparagus asparagoides Species 0.000 claims abstract description 20
- 241000792500 Calvatia fenzlii Species 0.000 claims abstract description 20
- 229940018006 basil oil Drugs 0.000 claims abstract description 20
- 239000010619 basil oil Substances 0.000 claims abstract description 20
- 229930003779 Vitamin B12 Natural products 0.000 claims abstract description 13
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims abstract description 13
- 239000011715 vitamin B12 Substances 0.000 claims abstract description 13
- 235000019163 vitamin B12 Nutrition 0.000 claims abstract description 13
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims abstract description 12
- 239000000377 silicon dioxide Substances 0.000 claims description 20
- GSYSFVSGPABNNL-UHFFFAOYSA-N methyl 2-dimethoxyphosphoryl-2-(phenylmethoxycarbonylamino)acetate Chemical group COC(=O)C(P(=O)(OC)OC)NC(=O)OCC1=CC=CC=C1 GSYSFVSGPABNNL-UHFFFAOYSA-N 0.000 claims description 16
- 238000000265 homogenisation Methods 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 claims description 12
- CKLJMWTZIZZHCS-UWTATZPHSA-N D-aspartic acid Chemical compound OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 claims description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 11
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 235000013343 vitamin Nutrition 0.000 claims description 8
- 239000011782 vitamin Substances 0.000 claims description 8
- 229930003231 vitamin Natural products 0.000 claims description 8
- 229940088594 vitamin Drugs 0.000 claims description 8
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 8
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 6
- 239000011975 tartaric acid Substances 0.000 claims description 6
- 235000002906 tartaric acid Nutrition 0.000 claims description 6
- 239000004310 lactic acid Substances 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 4
- 239000004471 Glycine Substances 0.000 claims description 4
- 235000015165 citric acid Nutrition 0.000 claims description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 2
- 241001529734 Ocimum Species 0.000 claims description 2
- 235000010676 Ocimum basilicum Nutrition 0.000 claims description 2
- 244000223014 Syzygium aromaticum Species 0.000 claims description 2
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims 1
- 229910052804 chromium Inorganic materials 0.000 claims 1
- 239000011651 chromium Substances 0.000 claims 1
- 235000019504 cigarettes Nutrition 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 abstract description 6
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 4
- 241000235342 Saccharomycetes Species 0.000 abstract description 4
- 230000000452 restraining effect Effects 0.000 abstract description 4
- 230000001408 fungistatic effect Effects 0.000 abstract description 3
- 150000008564 D-lysines Chemical class 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 7
- 235000013305 food Nutrition 0.000 description 6
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 4
- 108010039918 Polylysine Proteins 0.000 description 3
- 229920000656 polylysine Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 241001251949 Xanthium sibiricum Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000254 damaging effect Effects 0.000 description 1
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 229960002163 hydrogen peroxide Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000004311 natamycin Substances 0.000 description 1
- 235000010298 natamycin Nutrition 0.000 description 1
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 description 1
- 229960003255 natamycin Drugs 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 235000017454 sodium diacetate Nutrition 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/34635—Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3553—Organic compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
本发明公开了一种复合生物防腐剂及其制备方法，该由复合生物防腐剂以下组分制备而成：N‑苄氧羰基‑D‑天冬氨酸、乳酸链球菌素、L‑多聚赖氨酸、维生素B12、烟酸铬、甘氨酸富马酸钠、丁香罗勒油、马勃菌粉、土茯苓粉、甘松粉、二氧化硅份、有机酸、冰片、EDTA二钠、蒸馏水。本发明提供的复合生物防腐剂的抑菌范围广，对革兰氏阴性菌、酵母菌、大肠杆菌和金黄色葡萄球菌均具有良好的抑菌效果，对环境pH和温度依赖性较小，适用范围广泛。
Description
技术领域
本发明属于属于生物技术领域，具体涉及一种复合生物防腐剂及其制备方法。
背景技术
防腐剂是食品和饲料产品的关键添加剂之一，其直接影响着食品和饲料产品的保存期和质量，减少产品在生产、加工、运输及贮存过程中，由于各种菌种或毒素对产品造成不同程度的污染从而导致经济损失。对防腐剂的研究也成为食品和饲料产品领域的研发和应用热点之一，传统的防腐剂为化学防腐剂，常见的有苯甲酸钠、山梨酸钾、脱氢乙酸钠、丙酸钙、双乙酸钠、乳酸钠、对羟基苯甲酸丙酯和过氧化氢等。有些防腐剂会被微生物分解，如山梨酸能被乳酸菌还原为山梨糖醇，可成为其碳源，促进微生物大量繁殖，并且这些化学防腐剂在食品和饲料中添加量过大也会引起一些不良反应。此外，这些化学防腐剂具有潜在的致突、致畸形及致癌等危害作用，对人类健康和生态环境存带来了严重的威胁和不利影响。
生物防腐剂由于其利用生物防腐材料本身具有的良好的抑菌作用，不会造成食品的毒素污染，并且有些生物防腐剂本身就是食品中的某种营养成分，相比与化学添加剂其具有无毒、安全等优点，应用前景良好。目前的生物防腐剂主要有乳酸链菌肽和纳他霉素两种，虽然两者具备良好的抑菌作用，但是其抑菌范围具有一定的局限性，对于革兰氏阴性菌、霉菌和酵母菌等抑制率较低，同时对于环境pH和温度具有一定的依赖性。
发明内容
为此，本发明提供一种复合生物防腐剂及其制备方法，解决现有技术中至少一种技术问题。
为此，本发明提供一种复合生物防腐剂，以重量份计，由以下组分制备而成：N-苄氧羰基-D-天冬氨酸20-38份、乳酸链球菌素3-15份、L-多聚赖氨酸2-11份、维生素B12 1-7份、烟酸铬1.2-5份、甘氨酸富马酸钠2-7份、丁香罗勒油0.6-1.9份、马勃菌粉2-4.2份、土茯苓粉0.4-1.3份、甘松粉0.6-2.2份、二氧化硅0.2-0.9份、有机酸2.4-5.6份、冰片0.5-2份、EDTA二钠1.8-3.1份、蒸馏水44-60份。
根据本发明的一个实施方式，其中，所述有机酸为柠檬酸、乳酸、果酸和甘氨酸中的一种或多种。
根据本发明的一个实施方式，其中，所述L-多聚赖氨酸的分子量为1-10万。
根据本发明的一个实施方式，其中，以重量份计，由以下组分制备而成：N-苄氧羰基-D-天冬氨酸22-36份、乳酸链球菌素3.5-14份、L-多聚赖氨酸2.6-10.2份、维生素B121.5-6.3份、烟酸铬1.7-4.5份、甘氨酸富马酸钠2.2-6.4份、丁香罗勒油0.8-1.5份、马勃菌粉2.3-4份、土茯苓粉0.6-1.2份、甘松粉0.9-1.8份、二氧化硅0.3-0.8份、有机酸2.6-5.3份、冰片0.6-1.7份、EDTA二钠1.9-2.8份、蒸馏水46-58份。
根据本发明的一个实施方式，其中，以重量份计，由以下组分制备而成：N-苄氧羰基-D-天冬氨酸34份、乳酸链球菌素10.8份、L-多聚赖氨酸6.7份、维生素B12 4.7份、烟酸铬3.2份、甘氨酸富马酸钠4.5份、丁香罗勒油1.4份、马勃菌粉3.2份、土茯苓粉0.9份、甘松粉1.4份、二氧化硅0.5份、有机酸4.2份、冰片1.5份、EDTA二钠2.4份、蒸馏水56份。
为此，本发明提供复合生物防腐剂的制备方法，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸20-38份、乳酸链球菌素3-15份、L-多聚赖氨酸2-11份、维生素B12 1-7份、烟酸铬1.2-5份、甘氨酸富马酸钠2-7份、丁香罗勒油0.6-1.9份、马勃菌粉2-4.2份、土茯苓粉0.4-1.3份、甘松粉0.6-2.2份、二氧化硅0.2-0.9份、有机酸2.4-5.6份、冰片0.5-2份、EDTA二钠1.8-3.1份、蒸馏水44-60份；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至50-70℃，搅拌15-40分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌30-60分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，灭菌即得。
根据本发明的一个实施方式，其中，所述均质处理的条件为：温度为35-42℃，时间为10-50min，压力为4-15MPa。
本发明的有益效果为：
本发明提供的复合生物防腐剂的抑菌范围广，对革兰氏阴性菌、酵母菌、大肠杆菌和金黄色葡萄球菌均具有良好的抑菌效果，且在pH为8.5的情况下对大肠杆菌的抑菌率仍维持在90%以上，在25℃环境下对金黄色葡萄球菌的的抑菌率也仍维持在90%以上，对环境pH和温度依赖性较小，适用范围广泛。
具体实施方式
下面结合实施例，对本发明作进一步详细描述。以下实施例用于说明本发明，但不用来限制本发明的范围。
实施例1
一种复合生物防腐剂的制备方法，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸20份、乳酸链球菌素3份、L-多聚赖氨酸2份、维生素B12 1份、烟酸铬1.2份、甘氨酸富马酸钠2份、丁香罗勒油0.6份、马勃菌粉2份、土茯苓粉0.4份、甘松粉0.6份、二氧化硅0.2份、有机酸2.4份、冰片0.5份、EDTA二钠1.8份、蒸馏水44份；其中，所述有机酸为质量比为1:2的果酸和甘氨酸的混合物；所述L-多聚赖氨酸的分子量为1-10万；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至50℃，搅拌15分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌30分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，其均质处理的条件为：温度为35℃，时间为10min，压力为4MPa，灭菌即得。
实施例2
一种复合生物防腐剂的制备方法，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸38份、乳酸链球菌素15份、L-多聚赖氨酸11份、维生素B12 7份、烟酸铬5份、甘氨酸富马酸钠7份、丁香罗勒油1.9份、马勃菌粉4.2份、土茯苓粉1.3份、甘松粉2.2份、二氧化硅0.9份、有机酸5.6份、冰片2份、EDTA二钠3.1份、蒸馏水60份；其中，所述有机酸为果酸；所述L-多聚赖氨酸的分子量为1-10万；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至70℃，搅拌40分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌60分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，其均质处理的条件为：温度为42℃，时间为50min，压力为15MPa，灭菌即得。
实施例3
一种复合生物防腐剂的制备方法，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸22份、乳酸链球菌素3.5份、L-多聚赖氨酸2.6份、维生素B12 1.5份、烟酸铬1.7份、甘氨酸富马酸钠2.2份、丁香罗勒油0.8份、马勃菌粉2.3份、土茯苓粉0.6份、甘松粉0.9份、二氧化硅0.3份、有机酸2.6份、冰片0.6份、EDTA二钠1.9份、蒸馏水46份；其中，所述有机酸为柠檬酸、乳酸、果酸和甘氨酸中的一种或多种；所述L-多聚赖氨酸的分子量为1-10万；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至60℃，搅拌25分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌45分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，其均质处理的条件为：温度为38℃，时间为25min，压力为12MPa，灭菌即得。
实施例4
一种复合生物防腐剂的制备方法，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸36份、乳酸链球菌素14份、L-多聚赖氨酸10.2份、维生素B12 6.3份、烟酸铬4.5份、甘氨酸富马酸钠6.4份、丁香罗勒油1.5份、马勃菌粉4份、土茯苓粉1.2份、甘松粉1.8份、二氧化硅0.8份、有机酸5.3份、冰片1.7份、EDTA二钠2.8份、蒸馏水58份；其中，所述有机酸为质量比为1:4:2的乳酸、果酸和甘氨酸的混合物；所述L-多聚赖氨酸的分子量为1-10万；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至65℃，搅拌22分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌48分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，其均质处理的条件为：温度为40℃，时间为45min，压力为12MPa，灭菌即得。
实施例5
一种复合生物防腐剂的制备方法，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸34份、乳酸链球菌素10.8份、L-多聚赖氨酸6.7份、维生素B12 4.7份、烟酸铬3.2份、甘氨酸富马酸钠4.5份、丁香罗勒油1.4份、马勃菌粉3.2份、土茯苓粉0.9份、甘松粉1.4份、二氧化硅0.5份、有机酸4.2份、冰片1.5份、EDTA二钠2.4份、蒸馏水56份；其中，所述有机酸为质量比为1:4的柠檬和甘氨酸；所述L-多聚赖氨酸的分子量为1-10万；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至65℃，搅拌20分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌55分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，其均质处理的条件为：温度为38℃，时间为35min，压力为12MPa，灭菌即得。
对比例1
本对比例与实施例1的不同之处在于：不添加烟酸铬和甘松粉。
对比例2
本对比例与实施例1的不同之处在于：不添加L-多聚赖氨酸、丁香罗勒油和马勃菌粉。
下面对实施例1至5以及对比例1、2所制备的生物防腐剂进行相关性能测试，其测试结果如下表所示：
| 抑菌率/%（革兰氏阴性菌） | 抑菌率/%(酵母菌) | 抑菌率/%（大肠杆菌，pH为8.5） | 抑菌率/%（金黄色葡萄球菌,25℃） | |
| 实施例1 | 86.8 | 87.1 | 91.4 | 90.6 |
| 实施例2 | 87.6 | 87.8 | 92.6 | 91.3 |
| 实施例3 | 88.7 | 88.4 | 93.2 | 91.7 |
| 实施例4 | 89.3 | 89.1 | 94.3 | 92.4 |
| 实施例5 | 90.4 | 90.1 | 95.1 | 92.8 |
| 对比例1 | 70.2 | 75.4 | 82.3 | 77.3 |
| 对比例2 | 64.5 | 73.5 | 81.5 | 80.1 |
通过上述测试结果可知，本发明提供的复合生物防腐剂的抑菌范围广，对革兰氏阴性菌、酵母菌、大肠杆菌和金黄色葡萄球菌均具有良好的抑菌效果，且在pH为8.5的情况下对大肠杆菌的抑菌率仍维持在90%以上，在25℃环境下对金黄色葡萄球菌的的抑菌率也仍维持在90%以上，对环境pH和温度依赖性较小，适用范围广泛。
Claims (7)
1. 一种复合生物防腐剂，其特征在于，以重量份计，由以下组分制备而成：N-苄氧羰基-D-天冬氨酸20-38份、乳酸链球菌素3-15份、L-多聚赖氨酸2-11份、维生素B12 1-7份、烟酸铬1.2-5份、甘氨酸富马酸钠2-7份、丁香罗勒油0.6-1.9份、马勃菌粉2-4.2份、土茯苓粉0.4-1.3份、甘松粉0.6-2.2份、二氧化硅0.2-0.9份、有机酸2.4-5.6份、冰片0.5-2份、EDTA二钠1.8-3.1份、蒸馏水44-60份。
2.如权利要求1所述的复合生物防腐剂，其特征在于，所述有机酸为柠檬酸、乳酸、果酸和甘氨酸中的一种或多种。
3.如权利要求1所述的复合生物防腐剂，其特征在于，所述L-多聚赖氨酸的分子量为1-10万。
4. 如权利要求1所述的复合生物防腐剂，其特征在于，以重量份计，由以下组分制备而成：N-苄氧羰基-D-天冬氨酸22-36份、乳酸链球菌素3.5-14份、L-多聚赖氨酸2.6-10.2份、维生素B12 1.5-6.3份、烟酸铬1.7-4.5份、甘氨酸富马酸钠2.2-6.4份、丁香罗勒油0.8-1.5份、马勃菌粉2.3-4份、土茯苓粉0.6-1.2份、甘松粉0.9-1.8份、二氧化硅0.3-0.8份、有机酸2.6-5.3份、冰片0.6-1.7份、EDTA二钠1.9-2.8份、蒸馏水46-58份。
5. 如权利要求1所述的复合生物防腐剂，其特征在于，以重量份计，由以下组分制备而成：N-苄氧羰基-D-天冬氨酸34份、乳酸链球菌素10.8份、L-多聚赖氨酸6.7份、维生素B124.7份、烟酸铬3.2份、甘氨酸富马酸钠4.5份、丁香罗勒油1.4份、马勃菌粉3.2份、土茯苓粉0.9份、甘松粉1.4份、二氧化硅0.5份、有机酸4.2份、冰片1.5份、EDTA二钠2.4份、蒸馏水56份。
6.如权利要求1至5任一所述的复合生物防腐剂的制备方法，其特征在于，包括以下制备步骤：
步骤1. 按重量份称取各原料：N-苄氧羰基-D-天冬氨酸20-38份、乳酸链球菌素3-15份、L-多聚赖氨酸2-11份、维生素B12 1-7份、烟酸铬1.2-5份、甘氨酸富马酸钠2-7份、丁香罗勒油0.6-1.9份、马勃菌粉2-4.2份、土茯苓粉0.4-1.3份、甘松粉0.6-2.2份、二氧化硅0.2-0.9份、有机酸2.4-5.6份、冰片0.5-2份、EDTA二钠1.8-3.1份、蒸馏水44-60份；
步骤2. 将马勃菌粉、土茯苓粉、甘松粉、二氧化硅与丁香罗勒油混合均匀，加热至50-70℃，搅拌15-40分钟，冷却至室温；
步骤3. 将甘氨酸富马酸钠、维生素B12、L-多聚赖氨酸、N-苄氧羰基-D-天冬氨酸、EDTA二钠、有机酸和蒸馏水常温混合搅拌30-60分钟；
步骤4. 将乳酸链球菌素、冰片和烟酸铬加入步骤3中的搅拌液中，混合均匀后与步骤2所制备的混合液混合均匀，并进行均质处理，灭菌即得。
7.如权利要求6所述的复合生物防腐剂的制备方法，其特征在于，所述均质处理的条件为：温度为35-42℃，时间为10-50min，压力为4-15MPa。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710493908.4A CN107212236A (zh) | 2017-06-26 | 2017-06-26 | 一种复合生物防腐剂及其制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710493908.4A CN107212236A (zh) | 2017-06-26 | 2017-06-26 | 一种复合生物防腐剂及其制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107212236A true CN107212236A (zh) | 2017-09-29 |
Family
ID=59950412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710493908.4A Pending CN107212236A (zh) | 2017-06-26 | 2017-06-26 | 一种复合生物防腐剂及其制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107212236A (zh) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021159038A (ja) * | 2020-04-02 | 2021-10-11 | 丸大食品株式会社 | 食肉製品製造方法 |
| WO2023061416A1 (zh) * | 2021-10-13 | 2023-04-20 | 温州肯恩大学 | 一种组合物及其抗菌应用 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1029552A1 (de) * | 1999-02-18 | 2000-08-23 | Arconia GmbH | Mittel, Verfahren, Vorrichtung sowie Verwendungen zum biologischen Entkeimen von Luft und Räumen mit Luft |
| CN101028133A (zh) * | 2006-02-27 | 2007-09-05 | 权力敏 | 食品生物防腐剂 |
| CN101606745A (zh) * | 2009-06-26 | 2009-12-23 | 广东省微生物研究所 | 复合生物防腐剂及其制备方法和应用 |
| CN105473001A (zh) * | 2013-07-31 | 2016-04-06 | Cj第一制糖株式会社 | 利用超高压的蔬菜类食品的制备方法 |
-
2017
- 2017-06-26 CN CN201710493908.4A patent/CN107212236A/zh active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1029552A1 (de) * | 1999-02-18 | 2000-08-23 | Arconia GmbH | Mittel, Verfahren, Vorrichtung sowie Verwendungen zum biologischen Entkeimen von Luft und Räumen mit Luft |
| CN101028133A (zh) * | 2006-02-27 | 2007-09-05 | 权力敏 | 食品生物防腐剂 |
| CN101606745A (zh) * | 2009-06-26 | 2009-12-23 | 广东省微生物研究所 | 复合生物防腐剂及其制备方法和应用 |
| CN105473001A (zh) * | 2013-07-31 | 2016-04-06 | Cj第一制糖株式会社 | 利用超高压的蔬菜类食品的制备方法 |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021159038A (ja) * | 2020-04-02 | 2021-10-11 | 丸大食品株式会社 | 食肉製品製造方法 |
| WO2023061416A1 (zh) * | 2021-10-13 | 2023-04-20 | 温州肯恩大学 | 一种组合物及其抗菌应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102485898B1 (ko) | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 | |
| CN107212236A (zh) | 一种复合生物防腐剂及其制备方法 | |
| CN103355730A (zh) | 一种乳酸链球菌素复合生物防腐剂及其制备方法 | |
| CN108641996A (zh) | 一种地衣芽孢杆菌的发酵培养基及其生产方法 | |
| CN105054217A (zh) | 一种食品用复配防腐剂 | |
| KR100566380B1 (ko) | 미생물 및 효소를 이용한 옥수수 글루텐의 가수분해 방법 | |
| CN101263924A (zh) | 一种天然食品防腐剂配方及制备方法 | |
| KR101483198B1 (ko) | 미강 발효를 이용한 미네랄 결정체 제조방법 | |
| CN105002110A (zh) | 复合微生物制剂及其在水华爆发水体处理中的应用 | |
| CN110419716B (zh) | 一种侧孢短芽孢杆菌抗菌肽微胶囊制备方法 | |
| CN104450506B (zh) | 厌氧血培养基、培养瓶及培养瓶的制备方法 | |
| CN110229868A (zh) | 一种抑制烟草疫霉菌的试验方法 | |
| CN104099387A (zh) | 一种制备淀粉糖的糖化工艺 | |
| KR20200037468A (ko) | 구연산을 통한 미생물 생존내구력 강화와 유산균 및 인산가용화균으로 구성된 복합미생물 대량생산 액상비료 및 그 제조방법 | |
| CN105950587B (zh) | 一种猪溶菌酶来源的饲用抗菌十二肽及其制备方法 | |
| CN104291288A (zh) | 一种制备磷酸二氢铵副产土豆专用复合肥的方法 | |
| CN106261464A (zh) | 一种扩大乳酸链球菌素杀菌范围和提高杀菌能力的配方 | |
| KR101643298B1 (ko) | 장류 소스에 함유된 바실러스 세레우스 포자를 발아시켜 저감화시키는 방법 및 이에 의해 제조된 장류 소스 | |
| KR101368390B1 (ko) | 식물조직 배양용 정제 배지 및 그 제조방법 | |
| CN110403002A (zh) | 一种鸡蛋干的防腐方法 | |
| CN110577441A (zh) | 一种用吸水链霉菌代谢产物腺嘌呤-9-d安沟糖苷发酵液复配氨基酸锌型植物营养剂 | |
| CN101787349B (zh) | 用于筛选蛋白酶生产菌的培养基的制备方法 | |
| CN110100876A (zh) | 复配防腐剂及其制备和应用 | |
| CN109609411A (zh) | 一种用于降解红霉素的微生物菌群及其制备方法 | |
| CN110872518B (zh) | 酸性土壤改良剂及其制备方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170929 |
|
| WD01 | Invention patent application deemed withdrawn after publication |