CN107158023A - Prevent and treat the pharmaceutical composition and preparation method of the viral hemorrhagic disease of grass carp and black carp - Google Patents
Prevent and treat the pharmaceutical composition and preparation method of the viral hemorrhagic disease of grass carp and black carp Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/205—Amine addition salts of organic acids; Inner quaternary ammonium salts, e.g. betaine, carnitine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
- A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
- A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
- A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
Abstract
The invention discloses a kind of pharmaceutical composition of viral hemorrhagic disease for preventing and treating grass carp and black carp and its preparation and application.The pharmaceutical composition includes following composition：Astragalus polyose, isoprinosine, vitamin C and Vitamin K3 powder.The preventing and treating grass carp and the pharmaceutical composition of the viral hemorrhagic disease of black carp that the present invention is provided are medicine for oral administration, and easy to use, cost is low, and the prevention effect to research of hemorrhage of grass carp may be up to 100%.
Description
Technical field
The invention belongs to aquatic products field, and in particular to the drug regimen of the viral hemorrhagic disease of a kind of preventing and treating grass carp and black carp
Thing and preparation method thereof.
Background technology
Grass carp, is favorite dish on national consumer's dining table, and price is low, nutritious, delicious flavour, but is being supported
Viral hemorrhagic disease most easily occurs in growing, the overall incidence of disease is 70% or so, and no good medicine treatment of once falling ill, and raiser is just
Bear the loss of heaviness.
Research of hemorrhage of grass carp cause of disease is reovirus, and the disease occurs rapidly, to spread extensively, period of disease is long, does not limit
Specification, the big feature of harmfulness.Annual 6-September part, water temperature is the most popular more than 26 DEG C.Water temperature is down to less than 25 DEG C, the state of an illness
Fade away.In equal fish, black carp can equally infect this disease.
First, the symptom of research of hemorrhage of grass carp and pathological change
Research of hemorrhage of grass carp is easy to obscure with bacteriosis, with often with old three sick mixed infections and integrate
Disease.Particularly it is more easy to obscure with bacteremic septicemia.Because there is red muscularity, to grass, black carp virus hemorrhagic disease just
Make a definite diagnosis disconnected of crucial importance, be to be related to the correct medication for the state of an illness to provide reliable basis.
Viral hemorrhagic disease has three types.A, red muscularity：Sick fish appearance is without obvious bleeding or is showed only as light
Micro- bleeding, tears the obvious large area red meat of the visible whole-body muscle of skin, simultaneously because losing blood seriously, the gill presents " white sometimes
The gill ".Enteron aisle is dissected without food, intestines are in cerise or purple.This type finds expression in the specification Grass carp of 10 cms；It is B, red
The red gill cover type of fin：The gill cover, oral cavity, eye socket, fin base and the crown of sick fish are alternately obvious congested, but muscle is not obvious, accidental point-like
It is congested.Dissect enteron aisle general in purple, enteral is without food, swelling, no inflation shape, and gill portion has been infected by bacterial " gill rot " or " opened
Skylight " shape, this type is common in 2 liang -0.5 jin of specification fingerling；C, enteritis type：Sick fish body table and the difference of good fish are little, nothing
With tail fin base mild hyperaemia below obvious or accidental eye socket, dissection is shown in that intestines are seriously congested, and whole enteron aisle is in scarlet or purple, intestines system
Film and fat have obvious point-like congested.Enteral is without food, and no inflation, the visible intestines of severe patient are all dark purple, at the same fish glue wall and
Gall-bladder is full of the trace of blood, what is more, from anus when dissected watery blood as noted, and whole mesenterium and fat are serious red, this type rule
The commodity that 0.5-10 jins or so of lattice grass, black carp are all visible.In practice, three of the above type, can occur or be alternately present simultaneously.
2nd, correct diagnosis grass, black carp viral hemorrhagic disease
Research of hemorrhage of grass carp time of origin it is general 6-July more than 26 DEG C of water temperature (what is also had is initial in June mid-May
It is raw, have relation with temperature water temperature at that time and artificial factor environment), ill fish body table is with normal fish without too big difference Huo Ouyou areas
Not.Ill fish does not ingest in feeding, and only ceaselessly spins travelling around food platform, and sick fish starts slowly to swim side after five days,
Occurs death after a few days, this phenomenon shows the Grass carp of 1.5 liang -0.5 jin of small dimension, and big specification is only visible dead
Fish is died, but is loseed or rarely found travelling at the side of a pond.When the travelling fish of discovery be in the central single person in the pool, quickly second day it is visible dead
Die fish.
Appearance is not that it is obvious that the mild hyperaemia such as accidental eye socket, gill cover, but it is seriously congested to have suffered enteron aisle during dissection sick fish,
In purple or darkviolet, enteral loses intestines inflation, in swelling shape, anus is micro- red without food.Clinically in large size fingerling or business
It is 70% or so that the sick enteritis type of virus hemorrhagic, which occurs, for product fish to account for three types toatl proportion.
Once there occurs virus hemorrhagic disease in cultivation, infection is fast, and the course of disease is long, and the death rate is high, there is many families of breeding fish every year
The fish death rate is up to 60% or so.This disease infection just stops ingesting after 2 days, and the death time determines according to the constitution of fish, from infection
To dead constitution powerhouse 7-10 days, weak person 5-7 days or so, if so diagnostic error, inappropriate medication, its death time can grow
For 2 months.
According to above state of an illness feature, feature, on the time, can correctly it distinguish and diagnosing bacterial disease and viral hemorrhagic
Disease.
It is considered that research of hemorrhage of grass carp only occurs in fingerling then, due to the factor of the reach of science environment,
Plus the cultivation of large-scale feed, the artificial natural law for changing fish growth has cultivated 8-12 jin of the big specification of 2-3 years
Grass, black carp, can equally infect viral hemorrhagic sick (having case explanation).
For treatment research of hemorrhage of grass carp, aquatic science could not also find a kind of preferable therapeutic effect medicine so far
Thing.Currently available technology is only disclosed for this sick vaccine, but this vaccine has many weak points：1. it can only put
Carry out manually injecting one by one during seed；2. operation is fine, careless slightly, dosage is slightly larger, will dead fish；3. target is limited
System, can only use when putting seed, can not be again carried out in breeding process；4. using after vaccine, it is impossible to ensure hemorrhage not
Recurrence；5. labor intensity is big, the time is long.
The content of the invention
In order to solve the above-mentioned technical problem, the present inventor is utilized from the condition on waterman's work, during the last ten years in base
Layer has carried out the clinical trial of thousands of, completes the pharmaceutical composition for specially controlling grass carp virus hemorrhagic disease of the present invention, it is interior
Medication, easy to use, cure rate is high.
The invention provides a kind of pharmaceutical composition for the viral hemorrhagic disease for preventing and treating grass carp and black carp, including as follows into
Point：Astragalus polyose, isoprinosine, vitamin C and Vitamin K3 powder.
Preferably above-mentioned preventing and treating grass carp and the pharmaceutical composition of the viral hemorrhagic disease of black carp, in addition to：Radix Isatidis.
Preferably, each composition that the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp and black carp includes is in the preventing and treating
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of grass carp and black carp：
It is highly preferred that the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp and black carp also includes Radix Isatidis, it is in institute
State 10~30wt% of ratio shared in the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp and black carp.
It is highly preferred that each composition is shared in the pharmaceutical composition of the preventing and treating grass carp and the viral hemorrhagic disease of black carp
Ratio is as follows：
The present invention also provides the preparation method of the pharmaceutical composition of the described viral hemorrhagic disease for preventing and treating grass carp and black carp,
Characterized in that, taking each composition to mix.
The present invention also provides the application of the pharmaceutical composition of the viral hemorrhagic disease of above-mentioned preventing and treating grass carp and black carp, described anti-
The pharmaceutical composition mix of viral hemorrhagic disease of grass carp and black carp is controlled in fish meal, wherein the preventing and treating grass carp and black carp
The weight of the pharmaceutical composition of viral hemorrhagic disease is 0.1wt%~1wt% of fish meal weight.
The present invention can reach following technique effect：
1st, the preventing and treating grass carp and the pharmaceutical composition of the viral hemorrhagic disease of black carp that the present invention is provided are medicine for oral administration, easy to use,
Cost is low.
2nd, the present invention is provided preventing and treating grass carp and the pharmaceutical composition of the viral hemorrhagic disease of black carp, make full use of various medicines
Function composition, the illicit drug of no national regulation.
The bright spot of the pharmaceutical composition of the preventing and treating grass carp of the present invention and the viral hemorrhagic disease of black carp：A prevents grass carp and green grass or young crops
Fish disease toxicity hemorrhage instant effect, effect is good；B. without any toxicity and side effects, noresidue；C. it is environmentally friendly, meet the nuisanceless of national regulation
Fish medicine；D. safe range is big, food fish safety.E. not by region, environment, temporal limitation is used.
The characteristics of pharmaceutical composition of the preventing and treating grass carp of the present invention and the viral hemorrhagic disease of black carp：A. grass carp and green grass or young crops are prevented
The pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp that the alone present invention provides and black carp is only needed during fish disease toxicity hemorrhage
Mix fodder is for oral administration 4~6 days, and effect is significantly up to 100%；B. it is sick with bacillary hemorrhage complication in the event of viral hemorrhagic
Deng the preventing and treating grass carp provided with the present invention and the pharmaceutical composition and directed toward bacteria property hemorrhage of the viral hemorrhagic disease of black carp
Antibioticses or other medicines coordinate for oral administration 5~6 days, and cure rate is up to more than 90%.；If being C. to remove from big water surface aquaculture
The hardship of heavy labour, the preventing and treating grass carp that can provide the present invention and the processing of black carp viral hemorrhagic medicine composition are raised in fish
In material, directly feed, prevention grass carp and the sick effect of black carp viral hemorrhagic are notable；D. cost is low, the preventing and treating grass that the present invention is provided
Pharmaceutical chemistry material is made in the pharmaceutical composition of the viral hemorrhagic disease of fish and black carp, is used in conjunction 4-6 days, every hundred jin can prevent grass carp or
4000 jin or so of black carp；E. the preventing and treating grass carp and the pharmaceutical composition of the viral hemorrhagic disease of black carp that the present invention is provided compensate for grass
The many weak points of vaccine of fish virus hemorrhage.
3rd, the present invention provide preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition its to grass carp and black carp
The preventing and treating of viral hemorrhagic disease, short treating period (5~7 days), no recurrence, and drug resistance and the resistance to the action of a drug will not be produced to fish, it can grow
Phase Reusability.
Embodiment
With reference to specific embodiment, the invention will be further described, so that those skilled in the art can be more preferable
Understand the present invention and can be practiced, but illustrated embodiment is not as a limitation of the invention.
The invention provides a kind of pharmaceutical composition for the viral hemorrhagic disease for preventing and treating grass carp and black carp, including as follows into
Point：Astragalus polyose, isoprinosine, vitamin C and Vitamin K3, may also include collaboration auxiliary element Radix Isatidis.This composition is
Antiviral immunoassay product, the banning drugs of no national regulation.
A. astragalus polyose is immunologic active material.With antiviral agent, clearing heat and detoxicating tonifying Qi establishing-Yang, strengthening vital QI to eliminate pathogenic factors, energy
The potential disease-resistant factor in animal body is activated, can induced animal body generation interferon, enhancing immunologic function.Data shows, extensively
Spectrum is antiviral, and the viral enteritis disease, upper respiratory tract parvovirus to animal play the role of significantly to reduce and resist disease.
There are auxiliary well and increase curative effect effect in treatment, shared with other medicines, synergy can be played.
B. Vitamin K3 powder, feed addictive.Major function contains clotting factor, can promote the solidification of animal blood,
Hemorrhagic disease is treated, bleeding incessantly has good anastalsis.
C. vitamin C, feed addictive.Main function can quickly supplement grass carp and lack disease caused by C and cause fish disease wind
The increase of danger.VC levels have very strong effect to the immunologic function and resistance courses of infection of grass carp, and fish body is improved so as to reach
Resistance against diseases, prevents the stress reaction of fish and dead；Prevention and scurvy, anaemia, the inflammation of internal organs for the treatment of aquatic livestock
Disease.
D. isoprinosine, Chinese invention patent.In Japan's listing, successively listed again in the U.S., Italy within 1988.The production
The advantage of product and effect：1st, can simultaneously it be shared with multi-medicament perhaps, with collaboration summation action；2nd, adding the product has substantially
Improve the curative effect effect of existing product；3rd, the product is without species specificity, and broad-spectrum antiviral immunologic function is strong, complete scope is big；
4th, instant effect, 1 hour haemoconcentration peaks value, after medication 2 hours, with urea and uric acid excretion, does not measure after testing, nothing
Residual.
E. Radix Isatidis has antiviral agent as collaboration auxiliary element.Enter liver, stomach, the work(with clearing heat and cooling blood, removing toxic substances
Effect, liver and gallbladder protection plays prevention virus function using antiviral agent is generated, shared with other medicines, can play addition collaboration
Effect.
Consider from palatability：Two kinds of Chinese medicines of the present invention, astragalus polyose, Radix Isatidis, which have, is adapted to the agreeable to the taste of aquatic livestock
Property, astragalus polysaccharide extract sweet taste type, Radix Isatidis leaf can graze fish, and other 3 kinds are chemical agent, two kinds of Vitamin K3, vitamin C
For feed addictive, fish is without what uncomfortable or repellency, isoprinosine also You nothing unsuitable taste, for grass carp
This 5 kinds of medicines are the palatabilities of entirely appropriate grass carp.
Consider from drug effect：Astragalus polyose, Radix Isatidis, VC, four kinds of medicines of isoprinosine all have antiviral dive simultaneously
In Immunity active factor.The antiviral effect of the advantage of five kinds of components is tightly blended together by the present invention, transfers mutually
Strength one of iron walls and brass partitions of formation, prevent the invasion of virus, so as to reach purpose and the prevention for the treatment of.
With specific embodiment, the present invention will be described below.
Embodiment 1
The present embodiment preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition, each composition it is described preventing and treating grass carp and
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of black carp：
The preparation method of the pharmaceutical composition of the preventing and treating grass carp of the present embodiment and the viral hemorrhagic disease of black carp is as follows：Take each
Composition is mixed.
The application of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp：
Case (one) Sanshui District of Guangzhou Foshan City
Measure 30,000 jin or so (food property fishes) on 138 mu of the water surface, 1.8 meters of the depth of water, the existing pool.
April 17, grass carp started morbidity, and dead 20 is a plurality of daily, and increase to May 7 600 are a plurality of, dead specification 0.8
- 2 jin of jin, medicining condition；Continuous to use idodine class, the spirit of the disease of Chinese medicine class grass carp five waits 5 days Enrofloxacins for oral administration, waited to no effect.
Diagnosis process：Grass carp body surface smooth, eye socket is congested, dissects 5, the micro- inflation of some Dis has a small amount of food,
Some enteron aisles take on a red color or aubergine without food, and anus is normal, cut off enteron aisle, intestinal wall has point-like congested and large area is congested.
Diagnostic result：For research of hemorrhage of grass carp and bacillary hemorrhage complication.
Medicine：The preventing and treating grass carp of the present embodiment and the Ke Jiaen of pharmaceutical composition 100 of the viral hemorrhagic disease of black carp
Promise sand star 100 grams of 80 jin of (active constituent content 10%) mix fodders, every time 300 jin of companies feed 5 days, one day is secondary.
Tracking：May 9 started to take orally, and May 12 significantly reduced, the fish in May 14 fish pond stop it is dead, always with
Track did not rebounded or recurred to October, fish well-grown.
Case (two) Hubei Tianmen Chen Hutong prestige group cultivation base
Experimental group：Cultivate 70 mu of fish pond area, 2 meters of the depth of water, the existing very heavy left and right of pool food fish 10,000 5.
Incidence：May 8, grass carp morbidity was dead, daily averagely 150 a plurality of, 3 liang or so of specifications, and continuous medicine feed is had no result,
Feeding material is stopped in midway, starts feeding again after 12 days, death increases to daily 300 a plurality of, once used outer bold and vigorous sterilization virucidal agents
Plus Enrofloxacin antibiotic medicine for oral administration.10,000 jin or so of dead fish before being diagnosed for 16 days from onset of illness to July according to record.
July 16 diagnosed：The normal body surface of the gill is normal, by dissection, and enteron aisle has a small amount of food or without food, and intestinal wall point-like is filled
Blood or large area are congested, and intestines are micro- swollen, in aubergine.
Diagnostic result：The sick and bacillary hemorrhage complication of viral hemorrhagic.
Diagnostic medicine：The preventing and treating grass carp of the present embodiment and 100 grams of the pharmaceutical composition plus fluorine of the viral hemorrhagic disease of black carp
Benzene Buddhist nun examines 100 grams (active constituent contents 10%), and to mix 80 jin of fish meals one day secondary, 100 jin every time, even feeds 6 days.
Tracking：No. 16 start to feed diagnostic medicine and mix fish meal and taken orally, No. 19 by original dead 300 a plurality of reduction extremely
150, the fish in No. 22 fish ponds stops dead.Trace into sell November and do not rebound, fish well-grown.
Control group 1：70 mu of fish pond area is cultivated, 2 meters of the depth of water deposits the pool 1.8 ten thousand jin of fish of food
Incidence：Grass carp morbidity on May 10, daily Mean Death 150 is a plurality of, 5 liang -1 jin of specification.
Diagnostic result：May 20 is by being diagnosed as research of hemorrhage of grass carp and bacillary hemorrhage complication
Diagnostic medicine：Florfenicol or Enrofloxacin are used alternatingly progress and take 100 grams to 200 grams (active constituent content 10%) orally
Mix 80 jin of fish meals, feed within one day it is secondary, 100 jin every time, even feed 10 days.
Tracking：It is continuous fish meal is mixed with antibioticses to feed up to 10 days, do not feel any better, death increase to it is 200 a plurality of, until
Still there was death on July 30.
Case (three) flat peach Yang Lin tails town
21 mu of fish pond, 2 meters of the depth of water, the existing pool measures 10,000 jin or so.
Incidence：Grass carp morbidity on May 15, starts dead 15 or so, grass carp, and peak period is up to 400 or so, specification
2-3 liang.
Therapeutic process：Trilogy and a large amount of used copper sulphate are combined during morbidity.Former powder (the active ingredient of Enrofloxacin for oral administration
Content 98%) every 40 kg diet is with 200 grams, 400 grams of K3 powder, and polysaccharide VC, hydrochloric acid sarafloxacin etc. are continuously using still to no effect.
Diagnostic result：June 10 is by being diagnosed as research of hemorrhage of grass carp and bacillary hemorrhage ancillary pathology.
Diagnostic medicine：The preventing and treating grass carp of the present embodiment and 100 grams of the pharmaceutical composition plus salt of the viral hemorrhagic disease of black carp
It is secondary that sour sarafloxacin 100 grams (active constituent contents 10%) mixes 80 jin of fish meals one day, 100 jin every time, even feeds 7 days.
Tracking：Started June 10 to mix fish meal and taken orally, June 13 a plurality of was reduced to more than 100 by original dead 400
Fish in bar, June 16 fish pond stops dead.Trace into sell November and do not rebound, fish well-grown.
Embodiment 2
The present embodiment preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition, each composition it is described preventing and treating grass carp and
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of black carp：
The preparation method of the pharmaceutical composition of the preventing and treating grass carp of the present embodiment and the viral hemorrhagic disease of black carp is as follows：Take each
Composition is mixed.
The application of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp：
Case：Hubei Hanchuan City lucky star town
260 mu of fish pond area, 1.7 meters of the depth of water are cultivated, eat food 320,000 jin of fish on the existing pool.
Incidence：Started the dead grass carp that falls ill, August 3 dead variegated carp, crucian, bream on July 22.
Death condition：Dead grass carp is up to 600 jin or so, 1.5 jin -11 jin of specification, more than 300 jins of grey silver carp etc. daily.
No. 9 diagnosis of August：Grass carp body surface is congested, and back caudal peduncle desquamation is serious and congested, and big grass carp has the bright red gill, dissects, abdomen
Water is serious, breaks anus watery blood direct current, liver and gall are especially big to wrap intestines and internal organ in khaki, some enteron aisles have a small amount of food or
Without food, intestines are in lavender and darkviolet, once with outer bold and vigorous sterilization virucidal agent and Enrofloxacin for oral administration, the antibiolics such as Florfenicol
To no effect.
Diagnostic result：Liver and gall syndrome causes the sick and bacillary comprehensive complication of hemorrhage of grass carp virus hemorrhagic.
Diagnostic medicine：100 grams of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp
Added with antibiotic hydrochloric acid sarafloxacin 100 grams (active constituent contents 10%) mixes 80 jin of fish meals, and daily 28 wrap (80 jin of dresses), daily
It is secondary, even feed 5 days.
Tracking：August 10 is mixed fish meal and taken orally, No. 15 dead fishes reduced in 85%, No. 17 fish ponds stop it is dead,
No recurrence bounce-back, fish well-grown always.
Embodiment 3
The present embodiment preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition, each composition it is described preventing and treating grass carp and
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of black carp：
The preparation method of the pharmaceutical composition of the preventing and treating grass carp of the present embodiment and the viral hemorrhagic disease of black carp is as follows：Take each
Composition is mixed.
The application of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp：
Case：Jing Zhou jujube woods paving town
Cultivate 52 mu of fish pond.On July 2nd, 2010 falls ill, and starts dead 3-5 and gradually increases, and highest death is up to 400
Left and right, 3 liang or so of specification, the death time is for 20 days.
Symptom：Body surface is in the rhizome of Chinese monkshood, and the rotten cheek, some body surfaces are intact, and color is normal, also there is head and the congested shape of fin base.Once medication
Desinsection, sterilization, Enrofloxacin for oral administration, fluorine this Buddhist nun examine a variety of medicines such as K3 powder, but do not feel any better.
Diagnosis：It was found that there is the bacillary rotten cheek, some enteron aisles have food, but in swelling shape, some enteron aisles are without food, and intestines are in purple
Color, anus is micro- red.According to above symptom, bacillary hemorrhage and viral hemorrhagic disease syndrome are diagnosed as.
Prescription：Sterilize water transfer.With the drug regimen for the viral hemorrhagic disease that grass carp and black carp are prevented and treated made from the present embodiment
100 grams of thing, 100 grams of hydrochloric acid sarafloxacin (active constituent content 10%), mixes 80 jin of fish meal, and one day secondary, even feeds 5 days.
Tracking：Start to reduce to 30 a plurality of, the fish stopping death in the 7th day fish pond after for oral administration 4 days, and do not recur always,
Fish well-grown.
Embodiment 4
The present embodiment preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition, each composition it is described preventing and treating grass carp and
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of black carp：
The preparation method of the pharmaceutical composition of the preventing and treating grass carp of the present embodiment and the viral hemorrhagic disease of black carp is as follows：Take each
Composition is mixed.
The application of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp：
Case (one)：Xiantao City Peng Chang towns
1.2 ten thousand jin or so of the existing pool of 28 mu of area, 2 meters of the depth of water of breeding fish
Incidence：Start morbidity on May 10th, 2011, dead 20 is a plurality of daily, to June 28, a dead wheat harvesting period, peak
Phase reaches that 200 is a plurality of daily, 1.5 jin or so of specification, has once used the externally applied drug of many pesticide-germicides, takes antibiotic En Nuosha orally
Star, Florfenicol etc., to no effect.
Symptom：The sick fish body table of diagnosis on July 1 is normal, by dissection, it is found that enteron aisle has a small amount of food, the inflation of intestines back segment has
Anterior intestine have point-like congested, intestines are in purple, swelling.
Diagnostic result：Viral hemorrhagic disease integrates complication with bacteremic septicemia.
Prescriptions：With 100 grams of preventing and treating grass carp virus hemorrhagic medicine composition made from the present embodiment, plus Florfenicol
It is for oral administration 5 days that 100 grams (effective ingredients 10%) mix 70 jin of fish meal, twice a day, stopped July 6 dead, and tracking to sale does not have
Rebound, fish well-grown.
Case (two)：Xiantao City Hu Chang towns
1.5 ten thousand jin or so of the existing pond fish of 30 mu of area, 1.6 meters of the depth of water of breeding fish
Incidence：Start morbidity on July 10th, 2011, it is dead 30 a plurality of daily, to August 5 days, dead nearly one month, peak
Phase reaches that 300 is a plurality of daily, and specification had once used outer antibiolics bold and vigorous and for oral administration, to no effect at 7 liang or so.
No. 6 diagnosis of August, sick fish has gill rot, and fish body table is congested, and back caudal peduncle desquamation is serious and congested, and dissection, ascites is tight
Weight, breaks anus watery blood direct current, and some enteron aisles have a small amount of food or without food, and intestines are in purple or darkviolet, have large area congested.
Diagnostic result：Viral hemorrhagic is sick with bacillary hemorrhage complication.
Prescriptions：August 7 days is with preventing and treating 100 grams of research of hemorrhage of grass carp pharmaceutical composition, grace made from the present embodiment
Promise sand star 100 grams (effective ingredients 10%) is mixed 80 jin of fish meal and taken orally 7 days, and twice a day, August stops death on 13rd, tracks to
Sell it is no rebound, fish well-grown.
Embodiment 5
The present embodiment preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition, each composition it is described preventing and treating grass carp and
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of black carp：
The preparation method of the pharmaceutical composition of the preventing and treating grass carp of the present embodiment and the viral hemorrhagic disease of black carp is as follows：Take each
Composition is mixed.
The application of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp：
Case (one)：Jingmen Zhanghe Reservoir
36 mouthfuls of net cage in 2010,6 × 6, the careless black carp of cultivation is main to bring specification up, and 8 jin of grass carp, 12 jin of black carp started hair on July 15
Disease, dead 5 daily, the dead grass in peak period, black carp is a plurality of up to 20.According to raiser, more than 9,000 jins of dead fish, is used during this period
Ribavirin, occrycetin, Enrofloxacin, K3 powder etc., have no effect.
August passes through diagnosis on 25th：Sick fish body table is intact, but eyes drum is prominent, tears skin, and flush is serious, and intestines are without food
Thing, intestines are in swelling back segment without inflation, and when dissected watery blood is as noted.
Diagnostic result：Research of hemorrhage of grass carp Severe.
Prescriptions：200 grams of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp,
100 jin of fish meal is mixed, two times a day, is used in conjunction 7 days.
Tracking：August starts to take orally on the 26th, and August starts to reduce on the 30th, is used in conjunction after 6 days, stops death, nothing is sold to November
Recurrence, fish well-grown.
Case (two)：Xiantao City in Hubei Province Peng Chang towns
26 mu of fishpond, 1.8 meters of the depth of water deposits 1.4 ten thousand jin or so of pond fish
On July 1st, 2011, grass carp started morbidity, and a plurality of 300 a plurality of, 8 days, 9 days, 10 days, three are risen to quickly by dead 10
Its common dead grass carp is a plurality of up to 7,000, daily more than 2,000,6-7 liang of specification.Period has used Enrofloxacin, Ribavirin, fluorobenzene Buddhist nun
The medicine such as examine, take orally and outer bold and vigorous bactericide, death does not subtract.
July 12 diagnosed, and sick fish body table is in black, and caudal peduncle fringing dissects a plurality of fish, enteron aisle without food or few food,
Enteron aisle is dark purple.
Diagnostic result：Bacillary hemorrhage syndrome caused by acute viral hemorrhage.
Prescriptions：100 grams of adapted hydrochloric acid sarafloxacins of research of hemorrhage of grass carp pharmaceutical composition that the present invention is provided
100 grams (active ingredients 10%) are mixed 100 jin of fish meal and taken orally, and one day secondary, is used in conjunction 6 days.
Tracking effect:Started July 12 to be taken orally with the pharmaceutical composition of the present invention, when for oral administration three days after, death die-off to
200 bars, stop dead, fish well-grown completely after the 7th day.
Embodiment 6
The present embodiment preventing and treating grass carp and black carp viral hemorrhagic disease pharmaceutical composition, each composition it is described preventing and treating grass carp and
Shared ratio is as follows in the pharmaceutical composition of the viral hemorrhagic disease of black carp：
The preparation method of the pharmaceutical composition of the preventing and treating grass carp of the present embodiment and the viral hemorrhagic disease of black carp is as follows：Take each
Composition is mixed.
The application of the pharmaceutical composition of the viral hemorrhagic disease of the obtained preventing and treating grass carp of the present embodiment and black carp：
Case (one)：Qianjiang Hao Kouzhen
20 mu of fishpond, 1.8 meters of the depth of water deposits the pool 1.5 ten thousand jin of fish of food
On May 15th, 2011, grass carp morbidity, started 25 or so, peak period is a plurality of up to 200,0.8-1.2 jin of specification, during which used
Outer bold and vigorous medicine, copper sulphate, ferrous sulfate, medicine for oral administration K3 powder, VC, astragalus polyose, plus Enrofloxacin antibiotic etc. are still dead tight
Weight.One, to June 18, cannot control death.
June 19 diagnosed, and sick fish body table is congested, and eye socket is congested, tail fringing.Dissection is a plurality of, it is found that enteron aisle has a small amount of food
Or without food, intestines are in cerise, what is had is in purple.
Diagnostic result：Bacillary hemorrhage integrates disease with research of hemorrhage of grass carp.
Diagnostic agent：It is husky with research of hemorrhage of grass carp pharmaceutical composition 150g adapteds hydrochloric acid is prevented and treated made from the present embodiment
Sand star 100g (active ingredient 10%) is drawn, 80 jin of fish meal is mixed and feeds for oral administration, twice a day, be used in conjunction 5 days.
Tracking effect：Started June 21 to take orally to June 27, also have death, even served again after 6 days, just stop dead.Through
Observation, never rebounds, fish well-grown.
Case (two)：Hubei Province Tianmen City Gan Yi towns
Existing 1.6 jin of the fish of pool food of 22 mu of fish pond, 1.8 meters of the depth of water
On June 5th, 2011, grass carp started morbidity, started dead 10 a plurality of, slowly reached 150 a plurality of, specification is at 7 liang or so.Examine
Before disconnected, medicine is sprinkled outside used pesticide-germicide, three blooms, VC, K3 powder, Florfenicol, the medicine for oral administration such as Ribavirin has also once used the earthwork
Method, ginger is endured water and splashed, invalid.
July 6 diagnosed：Dead fish body surface is slightly congested, gill blank, and irregular tail is in brush shape, and fin base is congested, dissection
Enteron aisle has point-like congested, the micro- inflation in intestines end, there is a small amount of food, and color is in aubergine, and mesenterium has hyperemia.
Diagnostic result：Research of hemorrhage of grass carp syndrome is switched to by grass carp " old three disease ".
Diagnostic medicine：With preventing and treating research of hemorrhage of grass carp pharmaceutical composition adapted Ciprofloxacin made from the present embodiment
It is for oral administration 6 days that 100 grams (active ingredients 10%) mix 80 jin of fish meal, two times a day.
Tracking effect：Started July 7 to take orally to July 13, still there is death, and then take orally to July 18, fish stopping
It is dead.It is observed that never rebound, fish well-grown.
Embodiment described above be only by absolutely prove the present invention and for being representative embodiment, it is of the invention
Protection domain not limited to this.Equivalent substitute or conversion that those skilled in the art are made on the basis of the present invention, exist
Within protection scope of the present invention.Protection scope of the present invention is defined by claims.
Claims (7)
1. a kind of pharmaceutical composition for the viral hemorrhagic disease for preventing and treating grass carp and black carp, it is characterised in that including following composition：It is yellow
Astragalus polysaccharides, isoprinosine, vitamin C and Vitamin K3 powder.
2. the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp according to claim 1 and black carp, it is characterised in that
Also include：Radix Isatidis.
3. the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp according to claim 1 and black carp, it is characterised in that
Disease of each composition that the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp and black carp includes in the preventing and treating grass carp and black carp
Shared ratio is as follows in the pharmaceutical composition of toxicity hemorrhage：
4. the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp according to claim 3 and black carp, it is characterised in that
Preventing and treating the pharmaceutical composition of the viral hemorrhagic disease of grass carp and black carp also includes Radix Isatidis, and it is in the preventing and treating grass carp and black carp
Shared 10~30wt% of ratio in the pharmaceutical composition of viral hemorrhagic disease.
5. the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp according to claim 4 and black carp, it is characterised in that
Each composition ratio shared in the pharmaceutical composition of the preventing and treating grass carp and the viral hemorrhagic disease of black carp is as follows：
6. the preparation of the pharmaceutical composition of the viral hemorrhagic disease of preventing and treating grass carp and black carp described in any one of Claims 1 to 5
Method, it is characterised in that take each composition to mix.
7. the application of the pharmaceutical composition of the viral hemorrhagic disease of any one of Claims 1 to 5 preventing and treating grass carp and black carp, its
It is characterised by, the pharmaceutical composition mix of the viral hemorrhagic disease of the preventing and treating grass carp and black carp is in fish meal, wherein described
The weight for preventing and treating the pharmaceutical composition of the viral hemorrhagic disease of grass carp and black carp is 0.1wt%~1wt% of fish meal weight.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113207903A (en) * | 2021-04-21 | 2021-08-06 | 清远海贝生物技术有限公司 | Chlorine dioxide effervescent tablet and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113207903A (en) * | 2021-04-21 | 2021-08-06 | 清远海贝生物技术有限公司 | Chlorine dioxide effervescent tablet and preparation method and application thereof |
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Application publication date: 20170915 |