CN107156840A - A kind of composition of the polysaccharide containing nostoc and its preparation method and application - Google Patents
A kind of composition of the polysaccharide containing nostoc and its preparation method and application Download PDFInfo
- Publication number
- CN107156840A CN107156840A CN201710355457.8A CN201710355457A CN107156840A CN 107156840 A CN107156840 A CN 107156840A CN 201710355457 A CN201710355457 A CN 201710355457A CN 107156840 A CN107156840 A CN 107156840A
- Authority
- CN
- China
- Prior art keywords
- nostoc
- polysaccharide
- composition
- parts
- sweet osmanthus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000192656 Nostoc Species 0.000 title claims abstract description 214
- 150000004676 glycans Chemical class 0.000 title claims abstract description 145
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 145
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 145
- 239000000203 mixture Substances 0.000 title claims abstract description 68
- 238000002360 preparation method Methods 0.000 title claims description 23
- 239000000284 extract Substances 0.000 claims abstract description 96
- 244000242564 Osmanthus fragrans Species 0.000 claims abstract description 72
- 235000019083 Osmanthus fragrans Nutrition 0.000 claims abstract description 72
- 210000002784 stomach Anatomy 0.000 claims abstract description 14
- 230000033228 biological regulation Effects 0.000 claims abstract description 8
- 210000001035 gastrointestinal tract Anatomy 0.000 claims abstract description 6
- 230000002265 prevention Effects 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 239000000843 powder Substances 0.000 claims description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- 239000012141 concentrate Substances 0.000 claims description 27
- 238000009413 insulation Methods 0.000 claims description 16
- 238000001556 precipitation Methods 0.000 claims description 14
- 238000005119 centrifugation Methods 0.000 claims description 13
- 235000013305 food Nutrition 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 239000003814 drug Substances 0.000 claims description 11
- 241000186000 Bifidobacterium Species 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 9
- 230000036541 health Effects 0.000 claims description 9
- 230000000968 intestinal effect Effects 0.000 claims description 9
- 235000009566 rice Nutrition 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 230000008719 thickening Effects 0.000 claims description 9
- 208000025865 Ulcer Diseases 0.000 claims description 8
- 230000001476 alcoholic effect Effects 0.000 claims description 8
- 231100000397 ulcer Toxicity 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 241000219780 Pueraria Species 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 1
- 208000007107 Stomach Ulcer Diseases 0.000 abstract description 7
- 201000005917 gastric ulcer Diseases 0.000 abstract description 7
- 238000012360 testing method Methods 0.000 description 72
- 241000699666 Mus <mouse, genus> Species 0.000 description 41
- 239000000243 solution Substances 0.000 description 25
- 206010010774 Constipation Diseases 0.000 description 23
- 230000000694 effects Effects 0.000 description 19
- 238000002474 experimental method Methods 0.000 description 18
- 230000037396 body weight Effects 0.000 description 17
- 241001465754 Metazoa Species 0.000 description 14
- 239000000976 ink Substances 0.000 description 13
- 239000003795 chemical substances by application Substances 0.000 description 12
- 235000013339 cereals Nutrition 0.000 description 10
- 235000019441 ethanol Nutrition 0.000 description 8
- 241000699670 Mus sp. Species 0.000 description 7
- 241000209094 Oryza Species 0.000 description 7
- 230000013872 defecation Effects 0.000 description 7
- 238000003304 gavage Methods 0.000 description 7
- 238000000643 oven drying Methods 0.000 description 7
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 210000000813 small intestine Anatomy 0.000 description 6
- 238000000108 ultra-filtration Methods 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000010171 animal model Methods 0.000 description 4
- 230000002550 fecal effect Effects 0.000 description 4
- 235000019082 Osmanthus Nutrition 0.000 description 3
- 241000333181 Osmanthus Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- -1 compound diphenoxylate Chemical class 0.000 description 3
- 229960004192 diphenoxylate Drugs 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 210000001187 pylorus Anatomy 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 241000193468 Clostridium perfringens Species 0.000 description 2
- 241000305071 Enterobacterales Species 0.000 description 2
- 241000194033 Enterococcus Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000008991 intestinal motility Effects 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical class [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 241000219991 Lythraceae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000004383 Steviol glycoside Substances 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000000762 glandular Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 208000003243 intestinal obstruction Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001809 melena Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 229920001206 natural gum Polymers 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229930182488 steviol glycoside Natural products 0.000 description 1
- 235000019411 steviol glycoside Nutrition 0.000 description 1
- 150000008144 steviol glycosides Chemical class 0.000 description 1
- 235000019202 steviosides Nutrition 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002408 ulcerprotective effect Effects 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 229940046001 vitamin b complex Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of composition of polysaccharide containing nostoc, the composition includes the component of following parts by weight, 80~100 parts of nostoc polysaccharide, 0~20 part of sweet osmanthus leaf extract.The composition of the polysaccharide of the present invention containing nostoc can effectively protect stomach lining, prevention and suppress gastric ulcer, improve bowel movement function, regulation gut flora etc..
Description
Technical field
The invention belongs to field of food, it is related to composition of a kind of polysaccharide containing nostoc and its preparation method and application.
Background technology
Nostoc is the spherical nostoc of plan (the Nostoc sphaeoides K ü in Nostoc (Nostoc Vauch)
Being commonly called as tzing).Also known as beauty's rice, day rosetangle, loosestrife, water agaric, field agaric, are that the edible one kind of China's tradition is precious
Wild food and medicament dual-purpose fresh water Azotica.Nostoc is with a long history in China, is not only one of nutritious traditional delicacies, and
And with higher medical treatment and health-care efficacy.According to records, nostoc has improving eyesight QI invigorating, antipyretic clear diaphragm, food macrobiosis, elimination long tired
The effects such as labor, convergence.Nostoc is rich in active polysaccharide, protein, vitamin B complex and micro mineral matter element.In edible
Algae in, nostoc is that nutriment is a kind of.
Nostoc polysaccharide is one of main component of nostoc, the good fluidity when polysaccharide has low concentration, during high concentration
Viscosity is high, the features such as molecular weight distribution is wide.Recent activity research shows, in nostoc polysaccharide have improve it is immune, anti-oxidant,
Suppress tumour, the effects such as.
The content of the invention
The present invention provides a kind of by the composition of raw material of nostoc polysaccharide and sweet osmanthus leaf extract and preparation method thereof, institute
Stating composition has protection stomach lining, regulation gut flora and the effect for improving bowel movement function, and long-term consumption has no side effect.
The composition for the polysaccharide containing nostoc that the present invention is provided includes the component of following parts by weight, and nostoc polysaccharide 80~
100 parts, 0~20 part of sweet osmanthus leaf extract.
Preferably, 85~95 parts of the nostoc polysaccharide, 5~15 parts of sweet osmanthus leaf extract.
It is further preferred that the composition of the polysaccharide containing nostoc includes 100 parts of nostoc polysaccharide；Or, it is described containing Pueraria lobota
The composition of celestial rice polysaccharide includes 95 parts of nostoc polysaccharide, 5 parts of sweet osmanthus leaf extract；Or, the combination of the polysaccharide containing nostoc
Thing includes 90 parts of nostoc polysaccharide, 10 parts of sweet osmanthus leaf extract；Or, the composition of the polysaccharide containing nostoc is more including nostoc
85 parts of sugar, 15 parts of sweet osmanthus leaf extract.
In the present invention, it can be prepared using the nostoc polysaccharide and sweet osmanthus leaf extract of commercialization described many containing nostoc
The composition of sugar.
Present invention also offers the preparation method of the composition of the polysaccharide containing nostoc, methods described includes：Two kinds of originals
The preparation method of material is as follows.
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) by nostoc fresh goods dry naturally or using air dry oven drying to moisture be 8~15%, obtain
Nostoc dry product.Preferably, the use of air dry oven drying to moisture is 8%, 10%, 15%.
3) nostoc dry product is crushed to below 100 mesh using pulverizer, obtains nostoc fine powder.Preferably, it is crushed to
Below 80 mesh；It is further preferred that being crushed to below 50 mesh.
4) nostoc fine powder soaks 4~20 hours by weight 300~600 times of water are added.Preferably, plus 500 times of water loggings
Bubble 12 hours；Or, 300 times of water soak 4 hours；Or, 450 times of water soak 16 hours；Or, 500 times of water soak 12 hours；Or, 600
Times water soaks 20 hours.
5) after the completion of soaking, 90~100 DEG C are heated to, 1~4h is extracted in insulation, obtains extract solution.Preferably, 90 are heated to
DEG C, 1h is extracted in insulation；Or, it is heated to 100 DEG C, insulation extraction 2h or 4h.
6) extract solution is concentrated into the 30%~35% of original weight amount using device for ultrafiltration membrane, obtains concentrate.Preferably, concentrate
30%, 33% or 35% to original weight amount.
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 30%~70%, that is, Ge Xian occurs
Rice polysaccharide precipitation.Preferably, mixed liquor alcoholic strength is 30%, 40%, 50%, 70%；
8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours.
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing.
It is prepared by sweet osmanthus leaf extract：
1) after fresh sweet osmanthus leaf harvesting, progress microwave de-enzyming drying, microwave power 500W~600W, fixation time 180s~
240s.Preferably, it is microwave power 500W, fixation time 240s；Or, it is preferable that it is microwave power 600W, fixation time
180s；Or, it is preferable that it is microwave power 550W, fixation time 210s.
2) the sweet osmanthus leaf dried is extracted 2 times by weight 10~20 times of water are added, and 50~60 DEG C of Extracting temperature is extracted every time
4~5h.Preferably, it is 55 DEG C of Extracting temperature, 4h is extracted every time；Or, it is preferable that it is 50 DEG C of Extracting temperature, 5h is extracted every time；
Or, it is preferable that it is 60 DEG C of Extracting temperature, 4h is extracted every time.
3) merge extract solution, after being removed slag through tube centrifuge centrifugation, carry out low temperature concentration, 50~60 DEG C of thickening temperature is dense
Extract solution solid content 35%~45% is reduced to, concentrate is obtained.Preferably, it is 50 DEG C of thickening temperature, is concentrated into and carries
Take liquid solid content 40%；Or, it is preferable that it is 55 DEG C of thickening temperature, is concentrated into extract solution solid content 35%；Or, it is excellent
Selection of land, is 60 DEG C of thickening temperature, is concentrated into extract solution solid content 45%.
4) concentrate is spray-dried obtains sweet osmanthus leaf extract.
Above two raw material is mixed in proportion, both the polysaccharide of the present invention containing nostoc composition.Preferably,
The composition of present invention polysaccharide containing nostoc is filling into bar bag using packing machine for powder, wherein, the polysaccharide containing nostoc
Composition presses 80~100 parts of nostoc polysaccharide, and 0~20 part of sweet osmanthus leaf extract is well mixed；Preferably, it is described many containing nostoc
The composition of sugar presses 85~95 parts of nostoc polysaccharide, and 5~15 parts of sweet osmanthus leaf extract is well mixed.It is further preferred that described
The composition of the polysaccharide containing nostoc includes 100 parts of nostoc polysaccharide；Or, the composition of the polysaccharide containing nostoc includes nostoc
90 parts of polysaccharide, 10 parts of sweet osmanthus leaf extract；Or, the composition of the polysaccharide containing nostoc includes 95 parts of nostoc polysaccharide, sweet osmanthus
5 parts of leaf extract；Or, the composition of the polysaccharide containing nostoc includes 85 parts of nostoc polysaccharide, 15 parts of sweet osmanthus leaf extract.
Present invention also offers a kind of preparation method of nostoc polysaccharide, methods described is as described above.
Present invention also offers a kind of nostoc polysaccharide prepared by the above method.
Present invention also offers a kind of preparation method of sweet osmanthus leaf extract, methods described is as described above.
Present invention also offers a kind of sweet osmanthus leaf extract prepared by the above method.
Present invention also offers a kind of composition of the polysaccharide containing nostoc prepared by the above method, the composition
Include the component of following parts by weight, 80~100 parts of nostoc polysaccharide, 0~20 part of sweet osmanthus leaf extract.
Preferably, the composition of the polysaccharide containing nostoc include 85~95 parts of nostoc polysaccharide, sweet osmanthus leaf extract 5~
15 parts.
It is further preferred that the composition of the polysaccharide containing nostoc includes 100 parts of nostoc polysaccharide.Or, it is described containing Pueraria lobota
The composition of celestial rice polysaccharide includes 95 parts of nostoc polysaccharide, 5 parts of sweet osmanthus leaf extract.Or, the combination of the polysaccharide containing nostoc
Thing includes 90 parts of nostoc polysaccharide, 10 parts of sweet osmanthus leaf extract.Or, 85 parts of the nostoc polysaccharide, 15 parts of sweet osmanthus leaf extract.
The composition of the polysaccharide containing nostoc of the present invention can also add nostoc dry powder, wherein, nostoc polysaccharide and Pueraria lobota
Celestial 80~100 parts of rice dry powder, 0~20 part of sweet osmanthus leaf extract.Wherein, the nostoc polysaccharide, the mass ratio of nostoc dry powder
For (30~90)：(10~70)；Preferably, the nostoc polysaccharide, the mass ratio of nostoc dry powder are (50~80)：(20~
50)；It is further preferred that the mass ratio of the nostoc polysaccharide, nostoc dry powder is 70：30.
Wherein, the preparation method of above-mentioned nostoc dry powder comprises the following steps：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) nostoc fresh goods is dried or using air dry oven drying to moisture 8~15% naturally, obtains nostoc
Dry product.It is preferred that, dried using air dry oven.
3) nostoc dry product is both obtained into nostoc dry powder using below pulverizer to 100 mesh.It is preferred that, it is crushed to 200 mesh
Below.
The composition of the polysaccharide containing nostoc of the present invention can also be added for components such as the fruit powders and steviol glycoside of flavoring.
Present invention also offers the composition of the above-mentioned polysaccharide containing nostoc in the food for preparing protection stomach lining, suppressing gastric ulcer
Application in product, medicine or health products.Preferably, the composition of the polysaccharide containing nostoc includes 100 parts of nostoc polysaccharide, osmanthus
0 part of floral leaf extract.Or, the composition of the polysaccharide containing nostoc includes 90 parts of nostoc polysaccharide, 10 parts of sweet osmanthus leaf extract.
Or, the composition of the polysaccharide containing nostoc includes 95 parts of nostoc polysaccharide, 5 parts of sweet osmanthus leaf extract.Or, it is described containing nostoc
The composition of polysaccharide includes 85 parts of nostoc polysaccharide, 15 parts of sweet osmanthus leaf extract.
The food, medicine or guarantor of preventing gastric ulcer are being prepared present invention also offers the composition of the above-mentioned polysaccharide containing nostoc
Application in strong product.
Present invention also offers the composition of the above-mentioned polysaccharide containing nostoc prepare improve the food of bowel movement function, medicine or
Application in health products；Preferably, for improving the first just time (defecation time first) for having constipation symptom object, excrement grain
Number, it is preferable that the composition of the polysaccharide containing nostoc includes 100 parts of nostoc polysaccharide, 0 part of sweet osmanthus leaf extract.Or, it is described
The composition of the polysaccharide containing nostoc includes 90 parts of nostoc polysaccharide, 10 parts of sweet osmanthus leaf extract.
Present invention also offers the composition of the above-mentioned polysaccharide containing nostoc prepare the regulation food of gut flora, medicine or
Application in health products.Preferably, the composition of the polysaccharide containing nostoc is used to adjust the enterobacteriaceaes such as Bifidobacterium, lactobacillus
Group.
The food of raising intestinal bifidobacteria quantity is being prepared present invention also offers the composition of the above-mentioned polysaccharide containing nostoc
Application in product, medicine or health products；Preferably, the composition of the polysaccharide containing nostoc includes 90 parts of nostoc polysaccharide, osmanthus
10 parts of floral leaf extract.
Raising intestinal bifidobacteria and lactobacillus are being prepared present invention also offers the composition of the above-mentioned polysaccharide containing nostoc
Application in food, medicine or the health products of quantity；Preferably, the composition of the polysaccharide containing nostoc includes nostoc polysaccharide
100 parts.
Contain Ge Xian the beneficial effects of the present invention are, what the present invention was provided include nostoc polysaccharide and sweet osmanthus leaf extract
The composition of rice polysaccharide has protection stomach lining, suppresses gastric ulcer, preventing gastric ulcer, improves bowel movement function, regulation gut flora
The effects such as.
Embodiment
With reference to specific examples below, the present invention is described in further detail.Implement the present invention process, condition,
Experimental method etc., is the universal knowledege and common knowledge of this area in addition to the following content specially referred to, the present invention does not have
Especially limit content.
Embodiment 1
100 parts of nostoc polysaccharide, 0 part of sweet osmanthus leaf extract both obtains the composition of product polysaccharide containing nostoc.
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) use air dry oven drying to moisture 8% nostoc fresh goods, obtain nostoc dry product
3) nostoc dry product is crushed to below 50 mesh using pulverizer, obtains nostoc fine powder.
4) nostoc fine powder adds 300 times of water to soak 4 hours.
5) after the completion of soaking, 90 DEG C are heated to, 1h is extracted in insulation, obtains extract solution.
6) extract solution is concentrated into the 30% of original weight amount using device for ultrafiltration membrane, obtains concentrate.
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 30%, that is, nostoc polysaccharide occurs
Precipitation.
8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours.
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing.
Embodiment 2
90 parts of nostoc polysaccharide, 10 parts of sweet osmanthus leaf extract is well mixed, and both obtains the combination of product polysaccharide containing nostoc
Thing.
The preparation method of above two raw material is as follows：
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) nostoc fresh goods is dried to moisture 15% naturally, obtains nostoc dry product
3) nostoc dry product is crushed to below 100 mesh using pulverizer, obtains nostoc fine powder.
4) nostoc fine powder adds 600 times of water to soak 20 hours.
5) after the completion of soaking, 100 DEG C are heated to, 4h is extracted in insulation, obtains extract solution.
6) extract solution is concentrated into the 35% of original weight amount using device for ultrafiltration membrane, obtains concentrate.
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 70%, that is, nostoc polysaccharide occurs
Precipitation.
8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours.
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing.
It is prepared by sweet osmanthus leaf extract：
1) after fresh sweet osmanthus leaf harvesting, microwave de-enzyming drying, microwave power 500W, fixation time 240s are carried out；
2) the sweet osmanthus leaf dried is extracted 2 times by weight 12 times of water are added, and 55 DEG C of Extracting temperature extracts 4h every time；
3) merge extract solution, after being removed slag through centrifugation, carry out low temperature concentration, 50 DEG C of thickening temperature is concentrated into extract solution solid
Thing content 40%, obtains concentrate；
4) concentrate is spray-dried obtains sweet osmanthus leaf extract.
Embodiment 3
95 parts of nostoc polysaccharide, 5 parts of sweet osmanthus leaf extract is well mixed, and both obtains the composition of product polysaccharide containing nostoc.
The preparation method of above two raw material is as follows：
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) use air dry oven drying to moisture 10% nostoc fresh goods, obtain nostoc dry product.
3) nostoc dry product is crushed to below 80 mesh using pulverizer, obtains nostoc fine powder.
4) nostoc fine powder adds 450 times of water to soak 16 hours.
5) after the completion of soaking, 100 DEG C are heated to, 2h is extracted in insulation, obtains extract solution.
6) extract solution is concentrated into the 33% of original weight amount using device for ultrafiltration membrane, obtains concentrate.
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 50%, that is, nostoc polysaccharide occurs
Precipitation.
8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours.
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing.
It is prepared by sweet osmanthus leaf extract：
1) after fresh sweet osmanthus leaf harvesting, microwave de-enzyming drying, microwave power 600W, fixation time 180s are carried out；
2) the sweet osmanthus leaf dried is extracted 2 times by weight 10 times of water are added, and 50 DEG C of Extracting temperature extracts 5h every time；
3) merge extract solution, after being removed slag through centrifugation, carry out low temperature concentration, 55 DEG C of thickening temperature is concentrated into extract solution solid
Thing content 35%, obtains concentrate；
4) concentrate is spray-dried obtains sweet osmanthus leaf extract.
Embodiment 4
85 parts of nostoc polysaccharide, 15 parts of sweet osmanthus leaf extract is well mixed, and both obtains the combination of product polysaccharide containing nostoc
Thing.
The preparation method of above two raw material is as follows：
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) use air dry oven drying to moisture 10% nostoc fresh goods, obtain nostoc dry product.
3) nostoc dry product is crushed to below 80 mesh using pulverizer, obtains nostoc fine powder.
4) nostoc fine powder adds 500 times of water to soak 12 hours.
5) after the completion of soaking, 100 DEG C are heated to, 2h is extracted in insulation, obtains extract solution.
6) extract solution is concentrated into the 35% of original weight amount using device for ultrafiltration membrane, obtains concentrate.
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 40%, that is, nostoc polysaccharide occurs
Precipitation.
8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours.
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing.
It is prepared by sweet osmanthus leaf extract：
1) after fresh sweet osmanthus leaf harvesting, microwave de-enzyming drying, microwave power 550W, fixation time 210s are carried out；
2) the sweet osmanthus leaf dried is extracted 2 times by weight 20 times of water are added, and 60 DEG C of Extracting temperature extracts 4h every time；
3) merge extract solution, after being removed slag through centrifugation, carry out low temperature concentration, 60 DEG C of thickening temperature is concentrated into extract solution solid
Thing content 45%, obtains concentrate；
4) concentrate is spray-dried obtains sweet osmanthus leaf extract.
Embodiment 5
56 parts of nostoc polysaccharide, 24 parts of nostoc dry powder, 20 parts of sweet osmanthus leaf extract is well mixed, and has both been obtained product and has been contained Pueraria lobota
It is celestial
The composition of rice polysaccharide.
The preparation method of above-mentioned three kinds of raw materials is as follows：
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) use air dry oven drying to moisture 10% nostoc fresh goods, obtain nostoc dry product.
3) nostoc dry product is crushed to below 80 mesh using pulverizer, obtains nostoc fine powder.
4) nostoc fine powder adds 500 times of water to soak 12 hours.
5) after the completion of soaking, 100 DEG C are heated to, 2h is extracted in insulation, obtains extract solution.
6) extract solution is concentrated into the 35% of original weight amount using device for ultrafiltration membrane, obtains concentrate.
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 40%, that is, nostoc polysaccharide occurs
Precipitation.
8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours.
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing.
The preparation of nostoc dry powder：
1) 3~6mm of diameter nostoc fresh goods is collected.
2) nostoc fresh goods is dried to moisture 15% naturally, obtains nostoc dry product
3) nostoc dry product is both obtained into nostoc dry powder using below pulverizer to 240 mesh.
It is prepared by sweet osmanthus leaf extract：
1) after fresh sweet osmanthus leaf harvesting, microwave de-enzyming drying, microwave power 550W, fixation time 210s are carried out；
2) the sweet osmanthus leaf dried is extracted 2 times by weight 20 times of water are added, and 60 DEG C of Extracting temperature extracts 4h every time；
3) merge extract solution, after being removed slag through centrifugation, carry out low temperature concentration, 60 DEG C of thickening temperature is concentrated into extract solution solid
Thing content 45%, obtains concentrate；
4) concentrate is spray-dried obtains sweet osmanthus leaf extract.
Embodiment 6
Embodiment 4 is repeated, its difference is that the composition for the polysaccharide containing nostoc for obtaining embodiment 4 is packaged into
Bar bag.
Embodiment 7
0 part of nostoc polysaccharide, 100 parts of sweet osmanthus leaf extract both obtains product.
The preparation method be the same as Example 4 of sweet osmanthus leaf extract.
The protection test that test example 1 is damaged to Mouse Gastric Mucous Membrane
1. given the test agent：The product that embodiment 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 7 are obtained.
2. decoction is configured：Given the test agent is configured to 20mg/ml suspension with distilled water.
3. experimental animal：ICR mouse, male, body weight 18-20g, Shanghai Slac Experimental Animal Co., Ltd. carries
For the animal quality certification：SCXK (Shanghai) 2012-0002.
4. animal packet：Experiment mice is divided into 7 groups, every group of 5 animals according to the excel completely random methods of dividision into groups.Packet is such as
Under：
Blank control group；Distilled water is administered；
Model group：Distilled water is administered；
Test group 1：The product of embodiment 1 is administered；
Test group 2：The product of embodiment 2 is administered；
Test group 3：The product of embodiment 3 is administered；
Test group 4：The product of embodiment 4 is administered；
Test group 5：The product of embodiment 7 is administered.
5. test method：
Mouse is by should give decoction oral gastric infusion, 1 times/day, 0.2ml/10g body weight, continuous 7 days.Before last dose
(the 7th day) fasting, it can't help water 24h.30min after administration, in addition to blank control group, other each group animal gavages give absolute ethyl alcohol
0.15ml/ is only.Cervical dislocation puts to death animal after 1h, ligatures pylorus, and 1% formaldehyde 1ml is injected into stomach body through glandular stomach portion, ligatures beautifully adorned
Door, takes out stomach, is immediately placed in 1% formaldehyde and fixes after 15min, splitted along greater curvature, normal saline flushing is clean, observes and determines
The damage of mouse stomach lining, calculates ulcer index and ulcer inhibition rate.
Ulcer inhibition rate calculation formula：
6. result of the test
Each test group of table 1. to ethanol cause mouse gastric ulcer protective effect (n=10,)
| Group | Use sample | Ulcer area (mm2) | Ulcer inhibition rate (%) |
| Blank control group | Distilled water | 0.25±0.27** | 96.71 |
| Model group | Distilled water | 7.66±1.79 | 0 |
| Test group 1 | The product of embodiment 1 | 3.78±2.41△ | 51.04 |
| Test group 2 | The product of embodiment 2 | 2.84±1.58△# | 62.92 |
| Test group 3 | The product of embodiment 3 | 3.02±2.69△# | 60.57 |
| Test group 4 | The product of embodiment 4 | 2.89±1.63△# | 62.27 |
| Test group 5 | The product of embodiment 7 | 6.83±2.24 | 10.84 |
Note：* represented compared with model group, P<0.01；△ represented compared with model group, P ＜ 0.01；# is represented and test group
1 compares, P ＜ 0.05.
The ulcer area of blank control group is substantially less than the ulcer area of model group, and two groups have the pole significance difference opposite sex (P ＜
0.01), illustrate that ethanol causes the model of mouse gastric ulcer to set up.1~test group of test group 4 (1~embodiment of embodiment 4) is to protection
Stomach lining has good effect, compared with model group, is respectively provided with pole significant difference (P ＜ 0.01).Test group 2, test group 3,
Test group 4 is respectively provided with significant difference (P ＜ 0.05) compared with test group 1, illustrates that nostoc polysaccharide is arranged in pairs or groups with sweet osmanthus leaf extract
Using having effects that coordinating protection stomach lining, its effect is significantly better than the effect that nostoc polysaccharide is used alone.Embodiment 5
Reach and the identical experiment effect of embodiment 2~4.
Test group 5 (embodiment 7) does not have remarkable result to protection stomach lining, compared with model group, is not significantly different, says
The bright sweet osmanthus leaf extract that is used alone is poor to the effect for protecting stomach lining.
Test example 2 improves the experiment of mice with constipation bowel movement function
1. given the test agent and processing method：Test group 1：The sample that embodiment 2 is obtained；Test group 2：What embodiment 1 was obtained
Sample；Test group 3：The sample that embodiment 7 is obtained.Given the test agent adds pure water to be configured to the suspension of 80mg/ml concentration.
2. experimental animal and packet：SPF grades of Kunming mouses 100, male, body weight 18-22g, Guangxi Medical University is real
Animal center offer, the animal quality certification are provided：SCXK (osmanthus) 2009-0002.Animal is divided into two groups, every group 50, experiment I groups are entered
Row intestinal motility is tested, and experiment II groups carry out the measure of defecation time, fecal grains and stool weight.Every group of experiment sets 3 examinations
Group is tested, while setting a blank control group and a Constipation Model control group.
3. test method：
3.1 intestinal motilities are tested
Male mice 50 is selected, 5 groups, every group 10 are randomly divided into.Three test groups give different given the test agent respectively
Suspension, blank control group and Constipation Model control group give isometric pure water, and gavage volume is 0.2ml/10g body weight, 1
Times/day, continuous gavage 7 days.Same day evening of the last to tested material, each group animal fasting (was drunk water and not limited) after 16 hours, constipation
Model control group and three test groups give the compound diphenoxylate solution (0.2ml/10g body weight) that dosage is 5mg/kg body weight
(compound diphenoxylate is used to cause mouse intestinal obstruction), blank control group gives pure water.After 30 minutes, three test groups are given and contained
The prepared Chinese ink of corresponding tested material, Constipation Model control group and blank control group give prepared Chinese ink (containing 5% activated carbon powder, 10% I
Uncle's natural gum).Give prepared Chinese ink after 25 minutes cervical dislocation put to death animal, open abdominal cavity separation mesenterium, clip upper end from pylorus, under
Hold to the intestinal tube of ileocecus, be placed on pallet, small intestine gently is pulled into straight line measures.Length of intestine is " small intestine overall length
Degree ", is " prepared Chinese ink length of recommended " to calculate ink progradation (P) by following equation, and carry out data to turn from pylorus to prepared Chinese ink forward position
Change statistical analysis again after (X).
The measure of 3.2 defecation times, fecal grains and stool weight
Select male mice 50, animal packet and give tested material method ibid 3.1 section.Same day evening of the last to tested material
On, the fasting of each group animal (was drunk water and not limited) after 16 hours, and Constipation Model control group and three test group gavages give 10mg/kg bodies
The compound diphenoxylate (0.2ml/10g body weight) of relatively heavy amount, blank control group gives the pure water of equivalent.After 30 minutes, model pair
Prepared Chinese ink gavage is given according to group and blank control group mouse, three test groups give the prepared Chinese ink of corresponding tested material, start simultaneously at timing.
Every animal is individually raised, and supplies feed and drinking-water, every animal of observed and recorded arrange melena first the time required to, 5 hours
Defecation grain number and gross weight.
4. result of the test
Influence of 4.1 samples to mouse weight
The changes of weight of each group mouse of table 2.
Note：I, II group are tested, the initial of each dosage group mouse, latter stage body weight and weightening are compared with blank control group, difference
There are no significant (P ＞ 0.05).
As seen from Table 2, the body weight of each group mouse is balanced before testing；After giving sample 7 days, the body weight of each test group mouse with
Blank control group and Constipation Model control group compare, difference there are no significant meaning, show that given the test agent increases to the body weight of mouse
Length is without influence.
Influence of 4.2 samples to mouse small intestine advancing movement
The small intestine ink progradation of each group mouse of table 3.
Note：P values in table are the ink progradation conversion value X of each group and the P values of Constipation Model control group comparing difference.
The ink progradation of Constipation Model control group mice is substantially less than blank control group, and two groups have pole significant difference (P
＜ 0.01), illustrate that Constipation Model is set up.The ink progradation of each test group mouse is above Constipation Model control group, through data
Statistical analysis after conversion, test group 1 (sample of embodiment 2) and test group 2 (sample of embodiment 1) and the difference of Constipation Model control group
It is different that there are conspicuousness (P ＜ 0.05), and data of the ink progradation higher than test group 2 of test group 1.Show the sample of embodiment 2
Product and the sample of embodiment 1 are respectively provided with the effect for promoting mouse small intestine advancing movement, and nostoc polysaccharide and sweet osmanthus leaf extract coordinate
The effect used is significantly better than the effect that nostoc polysaccharide is used alone.Embodiment 5 also reaches experiment effect same as Example 2
Really.
There was no significant difference between Constipation Model control group for test group 3 (sample of embodiment 7).Show that sweet osmanthus is used alone
Extract does not have remarkable effect to the advancing movement for promoting mouse small intestine.
Influence of 4.3 samples to mouse defecation
Defecation time, fecal grains and the weight of each group mouse of table 4.
Note：1st, P values are each group and the P values of Constipation Model control group comparing difference in table；
2nd, fecal grains and stool weight are the excrement for counting mouse in 5 hours.
Just the time is more than blank control group to the small mouse's head of Constipation Model control group, and both have the pole significance difference opposite sex (P ＜
0.01), and the grain number and stool weight of Constipation Model control group excrement is respectively less than blank control group, difference is respectively provided with conspicuousness
(P ＜ 0.01 and P ＜ 0.05 respectively), illustrates that Constipation Model is set up.First just time, test group 1 (sample of embodiment 2) and constipation mould
The difference of type control group has conspicuousness (P ＜ 0.05), and there was no significant difference with Constipation Model control group for other test groups；Excrement grain
Number, test group 1 (sample of embodiment 2) and test group 2 (sample of embodiment 1) and the difference of Constipation Model control group have conspicuousness (P
＜ 0.05), there was no significant difference with Constipation Model control group for test group 3；Stool weight, each test group and Constipation Model control group
No significant difference (P ＞ 0.05).Result above shows, the sample of embodiment 2 (90 parts of nostoc polysaccharide, sweet osmanthus leaf extract
10 parts) the first just time, and increase the grain number that mouse is discharged feces just of mouse can be shortened.Sample (the nostoc polysaccharide of embodiment 1
100 parts) mouse defecation grain number can be increased.The sample of embodiment 5 also reaches and the sample identical experiment effect of embodiment 2.
The sample of test example 3 adjusts the experiment of mouse intestinal flora
1. given the test agent and processing：Test group 1：The sample that embodiment 2 is obtained；Test group 2：The sample that embodiment 1 is obtained；
Test group 3：The sample that embodiment 7 is obtained.Given the test agent adds pure water to be configured to the suspension of 80mg/ml concentration.
2. experimental animal and packet：ICR mouse, male, body weight 18-20g, Shanghai Si Laike experimental animals Limited Liability is public
Department provides, the animal quality certification：SCXK (Shanghai) 2012-0002.If three test groups and a blank control group, every group 10 are moved
Thing.
3. test method
Male mice 40 is selected, 4 groups, every group 10 are randomly divided into.Three test groups give different given the test agent respectively
Suspension, blank control group gives isometric pure water, and gavage volume is 0.2ml/10g body weight, 1 times/day, continuous gavage 14
My god.Given before giving given the test agent with last time after given the test agent within 24h, respectively aseptic collection stool in mice
0.1g, add sterile diluent, 10 times are serially diluted, the suitable dilution factor of reselection, are seeded in respectively on each culture medium, by will
Ask and cultivated.To mouse weight, Bifidobacterium, lactobacillus, enterococcus, enterobacteria, the quantity of C.perfringens enter line number
According to processing and analysis.
4. result of the test
Influence of 4.1 samples to mouse weight
The changes of weight of each group mouse of table 5.
Note：The initial of each group mouse, latter stage body weight and weightening are compared with blank control group, there are no significant for difference (P ＞
0.05)。
As seen from Table 5, the body weight of each group mouse is balanced before testing；After continuously giving sample 14 days, each test group mouse
Body weight is compared with blank control group, difference there are no significant meaning, shows given the test agent on the body weight increase of mouse without influence.
Influence of 4.2 samples to mouse intestinal flora
The each group mouse intestinal flora of table 6. change (logcfu/g,N=10)
Note：P1 is is compared with blank control group before experiment, P2 is is compared with blank control group after experiment, P3 is before and after itself
Compare.
As shown in Table 6, compared after being tested before and after either testing with blank control group, or itself compared before and after experiment,
Each test group enterobacteria, enterococcus, the quantity of C.perfringens there are no significant difference (P ＞ 0.05)；Test group 1 (is implemented
The sample of example 2) itself the obvious increase before and after experiment of Bifidobacterium and lactobacillus quantity, difference has conspicuousness (P ＜
0.05), and after testing there is conspicuousness (P ＜ 0.05) with the obvious increase of blank group, difference.(the sample of embodiment 1 of test group 2
Product) bifidobacteria itself obvious increase before and after experiment, difference has a conspicuousness (P ＜ 0.05), and after experiment
With the obvious increase of blank group, difference has conspicuousness (P ＜ 0.05).Foundation《Health food examine with assessment technique specification-
2003》Middle regulation gut flora function decision method understands that the sample of embodiment 1 and the sample of embodiment 2 are respectively provided with regulation mouse intestines
The function of road flora.The sample of embodiment 5 also reaches and the sample identical experiment effect of embodiment 2.
The experiment shows：The composition (sample of embodiment 2) of nostoc polysaccharide and sweet osmanthus leaf extract can increase simultaneously
The quantity of Bifidobacterium and lactobacillus in mouse intestinal；And be used alone nostoc polysaccharide (sample of embodiment 1) merely add it is small
The quantity of Bifidobacterium in mouse enteron aisle；Sweet osmanthus leaf extract is used alone does not have effect to regulation mouse intestinal flora.
The protection content of the present invention is not limited to above example.Under the spirit and scope without departing substantially from inventive concept, this
Art personnel it is conceivable that change and advantage be all included in the present invention, and using appended claims as protect
Protect scope.
Claims (10)
1. the composition of a kind of polysaccharide containing nostoc, it is characterised in that the composition includes the component of following parts by weight, Pueraria lobota
Celestial 80~100 parts of rice polysaccharide, 0~20 part of sweet osmanthus leaf extract.
2. the composition of the polysaccharide as claimed in claim 1 containing nostoc, it is characterised in that it is many that the composition includes nostoc
85~95 parts of sugar, 5~15 parts of sweet osmanthus leaf extract.
3. the composition of the polysaccharide as claimed in claim 1 containing nostoc, it is characterised in that it is many that the composition includes nostoc
100 parts of sugar；Or, the composition includes 95 parts of nostoc polysaccharide, 5 parts of sweet osmanthus leaf extract；Or, the composition includes Ge Xian
90 parts of polysaccharide of rice, 10 parts of sweet osmanthus leaf extract；Or, the composition includes 85 parts of nostoc polysaccharide, 15 parts of sweet osmanthus leaf extract.
4. the composition of the polysaccharide as claimed in claim 1 containing nostoc, it is characterised in that the composition also includes nostoc
Dry powder, wherein, 80~100 parts of nostoc polysaccharide and nostoc dry powder, 0~20 part of sweet osmanthus leaf extract；Wherein, the nostoc
Polysaccharide, the mass ratio of nostoc dry powder are (30~90)：(10~70).
5. a kind of preparation method of the composition of polysaccharide containing nostoc, the described method comprises the following steps：
The preparation of nostoc polysaccharide：
1) 3~6mm of diameter nostoc fresh goods is collected；
2) it is 8~15% by nostoc fresh goods drying to moisture, obtains nostoc dry product；
3) nostoc dry product is crushed to below 100 mesh, obtains nostoc fine powder；
4) nostoc fine powder soaks 4~20 hours by weight 300~600 times of water are added；
5) after the completion of soaking, 90~100 DEG C are heated to, 1~4h is extracted in insulation, obtains extract solution；
6) extract solution is concentrated into the 30%~35% of extract solution weight using milipore filter, obtains concentrate；
7) edible ethanol mixing is added in concentrate, the alcoholic strength of mixed liquor is reached 30%~70%, that is, nostoc occurs many
Sugar precipitation；8) temperature of mixed liquor is down to 4 DEG C, insulation stands 6 hours；
9) precipitation is reclaimed in centrifugation, freeze-dried to obtain nostoc polysaccharide dry product, has both been nostoc polysaccharide after crushing；
It is prepared by sweet osmanthus leaf extract：
1) after fresh sweet osmanthus leaf harvesting, microwave de-enzyming drying, microwave power 500W~600W, fixation time 180s~240s are carried out；
2) the sweet osmanthus leaf dried is extracted 2 times by weight 10~20 times of water are added, 50~60 DEG C of Extracting temperature, extract 4 every time~
5h；
3) merge extract solution, after being removed slag through centrifugation, carry out low temperature concentration, 50~60 DEG C of thickening temperature is concentrated into extract solution solid
Thing content 35%~45%, obtains concentrate；
4) concentrate is spray-dried obtains sweet osmanthus leaf extract；
Above two raw material is mixed in proportion, and has both obtained the composition of the polysaccharide containing nostoc.
6. the composition for the polysaccharide containing nostoc that a kind of method as claimed in claim 5 is prepared, it is characterised in that described group
Compound includes the component by following parts by weight, 80~100 parts of nostoc polysaccharide, 0~20 part of sweet osmanthus leaf extract.
7. the composition of the polysaccharide containing nostoc as described in Claims 1 to 4,6 is preparing protection stomach lining, prevention and is suppressing stomach
Application in food, medicine or the health products of ulcer.
8. the composition of the polysaccharide containing nostoc as described in Claims 1 to 4,6 is preparing food, the medicine of improvement bowel movement function
Or the application in health products.
9. the composition of the polysaccharide containing nostoc as described in Claims 1 to 4,6 is preparing food, the medicine of regulation gut flora
Or the application in health products.
10. the composition of the polysaccharide containing nostoc as described in Claims 1 to 4,6 prepare improve intestinal bifidobacteria and/or
Application in food, medicine or the health products of lactobacillus quantity.
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