CN114366826A - Preparation method of larch arabinogalactan composition and sterilization treatment device thereof - Google Patents

Preparation method of larch arabinogalactan composition and sterilization treatment device thereof Download PDF

Info

Publication number
CN114366826A
CN114366826A CN202210108197.5A CN202210108197A CN114366826A CN 114366826 A CN114366826 A CN 114366826A CN 202210108197 A CN202210108197 A CN 202210108197A CN 114366826 A CN114366826 A CN 114366826A
Authority
CN
China
Prior art keywords
cabin
pressure
decompression
pressurizing
larch
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202210108197.5A
Other languages
Chinese (zh)
Inventor
郑双阳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dalian University
Original Assignee
Dalian University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dalian University filed Critical Dalian University
Priority to CN202210108197.5A priority Critical patent/CN114366826A/en
Publication of CN114366826A publication Critical patent/CN114366826A/en
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/733—Fructosans, e.g. inulin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
    • A61K9/148—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with compounds of unknown constitution, e.g. material from plants or animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/10—Apparatus features
    • A61L2202/12—Apparatus for isolating biocidal substances from the environment
    • A61L2202/121—Sealings, e.g. doors, covers, valves, sluices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
    • A61L2202/20—Targets to be treated
    • A61L2202/21—Pharmaceuticals, e.g. medicaments, artificial body parts

Abstract

The divisional application discloses a preparation method of larch arabinogalactan composition and a sterilization treatment device thereof. Belongs to the field of biotechnology and medicine, and is larch arabinogalactan composition comprising larch arabinogalactan 3-6 weight portions, inulin 0.5-3 weight portions, and coconut powder 0.4-3 weight portions. The preparation method comprises the following steps of S1, extracting arabinogalactan from larch; s2, preparing defatted coconut powder; s3, sterilizing a finished product; and (4) carrying out rapid sterilization treatment by using an instantaneous pressure-increasing and pressure-reducing sterilization treatment device to obtain the larch arabinogalactan composition. The preparation method has the advantages of low cost, simplicity and feasibility due to the adoption of static ultrahigh pressure and normal temperature treatment, can save a large amount of energy, and can completely and thoroughly extract the target substances. Clinical tests of volunteers prove that the larch arabinogalactan composition has good curative effect on diarrhea-type irritable bowel syndrome, and replaces a drug therapy with certain side effect in a food therapy mode.

Description

Preparation method of larch arabinogalactan composition and sterilization treatment device thereof
The application is divisional application of patent application with application number of 201910614589.7 and application date of 2019-7-9, which invents a preparation method of larch arabinogalactan composition and clinical application thereof.
Technical Field
The invention belongs to the field of biotechnology medicine, and relates to a compatibility, an extraction preparation method and an instantaneous pressure increasing and reducing sterilization technology of a larch arabinogalactan and defatted coconut powder composition; in particular to a preparation method of larch arabinogalactan composition and a sterilization treatment device thereof.
Background
The Arabinogalactans (AG) are present in a variety of plants, larch is the plant most abundant in arabinogalactans found in the world today, larch arabinogalactans are mainly distributed in the lumen of the tracheae of early and late wood of the heartwood part of larch, and are present in small amounts in ray cells and fat secreting cells, at least 90% of which, unlike other hemicelluloses, are located outside the larch cell wall: (
Figure BDA0003494082070000011
Etc., 1966;
Figure BDA0003494082070000012
and Westermark, 1999), mainly packed in tracheids, and therefore this fraction of extract is not a structural cell wall component (Grabner et al, 2005). Arabinogalactans have important physiological functions in plants. Loewus, after studying the conduit of the columella, suggested that arabinogalactan was nutritional because he observed the mucilaginous mass in the conduit filled with this polysaccharide as a carbohydrate donor during growth of the pollen tube cell wall. This is in fact what is generally considered to be polysaccharides as an adhesive and nutrient solution for anthers in plant flours (plant physiology letters, 1982, (3), 13-19). In plants arabinogalactans are often associated with proteins known as arabinogalactan proteins (AGP), which have the ability to bind large amounts of water and are therefore often used to explain the physiological function of some particular plants against frost damage, as well as the fact that under drought conditions the plant itself secretes a layer of jelly glue that encloses the cellsTo combat drought and protect cell membranes from desiccation. AGP is involved in many processes in plant growth and development, including somatic embryogenesis, root growth and development, hormonal responses, xylem differentiation, resistance to agrobacterium tumefaciens-mediated infection, initiation of female gametogenesis, salt tolerance, cell wall plasticizers, cell expansion, secretions, promotion of pollen tube growth and guidance, programmed cell death, pollen grain development, and self-incompatibility in pollen. In conclusion, arabinogalactans are an essential nutrient for many plants. Besides the physiological action of plants, the plant also has quite important physiological action on human bodies.
Many reports have been made on the extraction process of arabinogalactan from larch, including hot water extraction, microwave ultrasonic assisted extraction and the like. There are researchers who extract larch arabinogalactan with hot water at 60 ℃, and the structure and activity of polysaccharide molecules are affected by high temperature (food industry 2001, 33(7), 55).
A process for extracting arabinogalactan from larch includes such steps as pulverizing larch wood, immersing in petroleum ether for defatting, extracting with ionic liquid solution of 1-butyl-3-methylimidazole chloride to obtain liquid extract, adding it to alcohol solution to obtain deposit, filtering, and drying filter cake.
An arabinogalactan KMCP and its preparation method and application in preparing immunomodulator are described: defatting whole plant or any part of herba Ixeritis Denticulatae with ethanol, removing ethanol from the medicinal residue, extracting with water, concentrating the water extract, precipitating with ethanol, dissolving the precipitate with water, dialyzing, concentrating to obtain crude polysaccharide of herba Ixeritis Denticulatae, subjecting the crude polysaccharide of herba Ixeritis Denticulatae to DEAE cellulose chromatography, gradient eluting with NaCl solution, collecting fraction, concentrating, dialyzing, and freeze drying to obtain arabinogalactan KMCP (CN 108530547A).
A process (CN 104082423) for the simultaneous production of natural coconut oil and low fat coconut water: coconut oil and coconut milk beverage is obtained by squeezing and centrifuging. The method for producing natural coconut oil and low-fat coconut milk comprises the following steps: grinding, squeezing and filtering coconut meat to obtain coconut milk, and centrifugally separating the coconut milk to obtain concentrated coconut pulp and coconut whey; centrifuging the concentrated coconut milk to obtain a semi-finished coconut oil product and coconut whey; vacuum drying the semi-finished coconut oil to obtain a natural coconut oil finished product; adding composite emulsifier, composite stabilizer, acidity regulator, sweetener and water into coconut whey, homogenizing, filling, sterilizing at high temperature, and cooling to room temperature to obtain low-fat coconut milk product.
A new preparation process of natural coconut powder (CN 104824582A), and specifically relates to a new preparation process of natural coconut powder. The process flow comprises the steps of trimming, peeling, cleaning, spraying, crushing, juicing, filtering, burdening, filtering, homogenizing, spray-drying, re-drying, cooling, screening powder, bagging, weighing, sewing and warehousing. The coconut powder prepared by the method has uniform quality, good stability, high dispersity and good solubility. The technical scheme is that the coconut powder obtained by the technical scheme contains a large amount of fat because the coconut powder is not degreased.
Irritable Bowel Syndrome (IBS) is a group of bowel dysfunction diseases with persistent or intermittent episodes, characterized by changes in abdominal pain, abdominal distension, bowel habits and/or stool behavior to clinical manifestations, but a lack of structural and biochemical abnormalities in the gastrointestinal tract. Irritable bowel syndrome is common, the prevalence rate is between 2% and 50% worldwide, the prevalence rate is between 9% and 20% in western countries, 10-15% in the United states, and female is 2 times of male; in Chinese surveys, the prevalence rate in Beijing area is about 7.26%, and the prevalence rate in Guangdong province is about 11.5%; factors influencing the prevalence rate of the irritable bowel syndrome are related to intestinal infection, dietary factors, mental factors and the like, and researches show that brain workers are more prone to suffering from irritable bowel syndrome than physical workers. The incidence of irritable bowel syndrome is only second to cold and is a category of functional bowel disease, patients mainly suffer from middle-aged and young people, the incidence age is usually 20-50 years old, women are more common than men, the family aggregation tendency is existed, and the irritable bowel syndrome is often accompanied with other gastrointestinal dysfunction diseases such as functional dyspepsia. IBS is divided into four clinical types of diarrhea type, constipation type, mixed type and indeterminate type according to the characteristics of stool, and diarrhea is the main type in China. The etiology and pathogenesis of IBS are not well understood and are believed to be the result of the combined action of multiple factors, such as abnormal gastrointestinal motility, abnormal visceral sensation, abnormal control of the brain and bowel, inflammation and psychology. Diarrhea-predominant IBS shows no specific symptoms clinically, but has several features relative to organic gastrointestinal disease: the onset is slow, the attack is intermittent, the disease course is long, but the general health condition is not influenced, the appearance or aggravation of symptoms is usually related to mental factors or stress states, the symptoms are obvious in the daytime, and the symptoms are relieved after sleeping at night. Diarrhea: continuous or intermittent diarrhea, less excrement, paste and large amount of mucus; ② after 72 hours of fasting the symptoms disappear; the diseases do not appear at night and are different from organic diseases; part of patients can be induced by eating; patients may have alternating diarrhea and constipation.
The diagnostic criteria for IBS are based on symptomatology, and the diagnosis is based on the elimination of organic diseases, and the current internationally recognized diagnostic criteria for IBS RoIII are recommended: recurrent episodes of abdominal pain or discomfort (discomfort means feeling uncomfortable and not pain), with symptoms appearing at least 3 days per month during the last 3 months, combining 2 or more of: relieving symptoms after defecation; ② the defecation frequency is changed when the attack occurs; ③ attack with stool characteristics (appearance) change. Symptoms appear for at least 6 months before diagnosis, and the standard is met in nearly 3 months.
The treatment measures are as follows: current treatments for IBS are limited to symptomatic treatment. The gastrointestinal dynamics group of the digestive diseases of the Chinese medical society is put forward in the consensus opinion on the diagnosis and treatment of irritable bowel syndrome: the aim of treatment is to eliminate the worry of patients, improve symptoms and improve the quality of life. The treatment principle is based on good doctor-patient relationship, and is based on symptom treatment according to the main symptom type and graded treatment according to the symptom severity. Attention is paid to the individualization and comprehensive application of the treatment measures. Firstly, diet adjustment, secondly psychological and behavioral therapy, and thirdly drug therapy: antidiarrheal agents such as loperamide or compound diphenoxylate can improve diarrhea, and need to pay attention to adverse reactions such as constipation and abdominal distension. For mild patients, adsorbent such as octahedral montmorillonite can be used.
In recent years, with the change of external environment and life style of people, the incidence rate of irritable bowel syndrome is increased year by year, and the number of people suffering from irritable bowel syndrome is increased continuously. The main clinical manifestations of irritable bowel syndrome are abdominal discomfort, abdominal distension and abdominal pain, which seriously affect the normal life of patients, reduce the quality of life and bring great living pressure to patients. Under the current medical background, no specific pathogenesis of irritable bowel syndrome is found, and the diagnosis of the irritable bowel syndrome mainly depends on relevant clinical symptoms and physical examination. But it has been shown by related studies and data that immune inflammatory responses in the gut, hypersensitivity of the gut, disruption of gut-associated barrier function and antagonism and competition of different gut flora and gut motility disorders may be associated with the onset of irritable bowel syndrome. With the intensive research, the relationship between irritable bowel syndrome and intestinal flora is found to be relatively close. A large amount of intestinal flora exists in an organism, and researches show that an important method for regulating the intestinal flora is to intervene intestinal probiotics.
At present, the medicine intake of the medical treatment method has a plurality of side effects, and a healthy food therapy method is urgently needed for treatment.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a preparation method of a larch arabinogalactan composition and a sterilization treatment device thereof. The degreased coconut powder is degreased by using an organic solvent, the degreased coconut powder basically does not contain grease, a 1000 Dalton hollow filter membrane is used for removing fructose, glucose, sucrose and the like, and cellulose and other macromolecular dietary fibers are reserved. Especially, the spray drying pretreatment adopts ultrahigh pressure homogenization, so that the degreased coconut powder particles reach the nano level and are easier to absorb. The composition prepared by the preparation method has functions of preventing and treating diarrhea-predominant irritable bowel syndrome.
The above purpose of the invention is realized by the following technical scheme:
a larch arabinogalactan composition comprises larch arabinogalactan 3-6 parts, inulin 0.5-3 parts, and coconut powder 0.4-3 parts.
The preparation method of the larch arabinogalactan composition comprises the following specific steps:
s1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles, wherein the water content of the wood chip particles is required to be 20-35%, treating and soaking the wood chip particles by using 4-10 times of organic solvent, continuously stirring for 4 hours, standing for 10-24 hours, centrifugally filtering, discarding filtrate to obtain filter residue, removing the organic solvent in the filter residue, adding 4 times of water into the filter residue, placing the filter residue into an ultrahigh pressure device, treating for 10-15 minutes by using 300 times of organic solvent under 600 MPa, centrifugally filtering to obtain filtrate, concentrating the filtrate to a certain volume, slowly adding ethanol to ensure that the ethanol concentration of the solution reaches 55-70%, stirring for 2-4 hours, standing for 8-20 hours, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate by using hot water, cooling, adding ethanol again to ensure that the ethanol concentration of the solution reaches 60-70%, stirring for 2-4 hr, standing for 8-20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.07-1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan;
s2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring out coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration in the coconut pulp slurry to reach 75-90%, placing the slurry in an ultrahigh pressure device for treatment for 10-20 minutes at 50-400 MPa, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the intercepted part and the filter residue B to be mixed, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure range of 50-150 MPa, homogenizing for 30 minutes, and spray-drying to obtain defatted coconut powder.
S3, sterilizing a finished product;
uniformly mixing 3-6 parts of larch arabinogalactan, 0.5-3 parts of inulin and 0.4-3 parts of coconut powder, and performing rapid sterilization treatment by using an instantaneous pressure increase and decrease sterilization treatment device to obtain the larch arabinogalactan composition.
Further, the organic solvent in step S1 is any one of ethanol, acetone, n-hexane, and petroleum ether.
Further, the instantaneous pressure and pressure increasing and reducing sterilization processing device in the steps S1 and S3 comprises a laval nozzle structure as a main body, and the laval nozzle structure comprises a pressure chamber, a straight pipe, a pressure chamber, a blower and an air dryer; the air outlet of the air dryer is connected with the air inlet of the blower, and the air outlet of the blower is connected with the air inlet of the pressurizing cabin; the pressurizing cabin and the depressurizing cabin are connected through a straight-flow pipe; a feeding hopper is arranged at the upper part of the pressurizing cabin, and a discharge hole is arranged at the bottom of the decompression cabin; temperature sensors and pressure sensors are arranged in the pressurizing cabin and the decompression cabin respectively, annular temperature control pipes are arranged in the external cabin bodies of the pressurizing cabin and the decompression cabin, and sealing electromagnetic valves are arranged at the joints of the pressurizing cabin, the blower, the feeding hopper and the direct current pipe; the joints of the decompression chamber, the straight flow pipe and the discharge hole are provided with sealing electromagnetic valves; the pressure sensor in the pressurizing cabin is connected with the pressurizing pump; the decompression chamber internal pressure sensor is connected to the decompression pump.
Furthermore, the connection mode among all the components of the instantaneous pressure increase and decrease sterilization treatment device is space rigid closed type sealed pipeline connection.
Furthermore, the straight-flow pipe is 0.8m in length and is externally coated with a heat insulation layer.
Further, the process of the instant pressure increase and decrease sterilization treatment device comprises the following steps: (Larix Gmelini arabinogalactan is taken as an example)
A, common air is changed into dry air through an air dryer, the dry air enters an air blower, then an electromagnetic valve between the air blower and a pressurizing cabin connecting pipe is opened, and the dry air enters a pressurizing cabin;
b, opening an electromagnetic valve at a connecting pipe of the feeding hopper and the pressurizing cabin, and allowing the larch arabinogalactan particles to enter the pressurizing cabin from the feeding hopper;
c, regulating and controlling the temperature and pressure settings of the cabin of the pressurizing cabin and the cabin of the decompression cabin, wherein the constant temperature value in the cabin is implemented by a temperature sensor and a temperature control pipe laid on the outer surface of the cabin, and the constant pressure value in the cabin is implemented by a pressure sensor, a booster pump and a decompression pump;
d, when the temperature and the pressure of the cabin of the pressurizing cabin and the decompression cabin reach preset values, opening a direct current pipe electromagnetic valve connected between the pressurizing cabin and the decompression cabin, and allowing the dry air and the larch arabinogalactan particles to pass through a direct current pipe at a high flow rate and enter the decompression cabin from the pressurizing cabin;
e discharging the particles subjected to the transient variation of temperature and pressure, namely the sterilized particles from the discharge port of the decompression chamber.
Furthermore, in the process, the temperature in the pressurizing cabin is 80-85 ℃, and the pressure is 10 Mpa; the internal temperature of the decompression chamber is 18-23 ℃, and the pressure is 0.05 Mpa. The residence time of larch arabinogalactan particles in the pressurizing cabin is 2s, and the residence time of particles in the depressurizing cabin is 1 s. The driving gas for the particles in the cabin of the pressure cabin and the decompression cabin is dry air with a flow rate of 40 m/s.
The constant temperature control method of the pressure increasing cabin and the decompression cabin is realized by arranging annular temperature control pipes for the outer parts of the cabins of the pressure increasing cabin and the decompression cabin, arranging temperature sensors in the cabins of the pressure increasing cabin and the decompression cabin, and adopting sealing electromagnetic valves at the joints of the cabins of the pressure increasing cabin and the decompression cabin, the outside and other parts. An annular temperature control pipe is arranged outside each cabin, and temperature sensors are arranged in the cabins of the pressure increasing cabin and the pressure reducing cabin, so that a reliable temperature environment is realized. The pressure environment of the chambers of the pressure increasing chamber and the pressure reducing chamber is realized by an air pressure increasing pump, a pressure reducing pump and a pressure sensor.
Compared with the prior art, the invention has the beneficial effects that:
1. larix Gmelini arabinogalactan is a fine, dry, off-white powder with a slightly sweet and mild pine-like smell. It is completely soluble in water or fruit juice, has low viscosity, and is easy to manage, especially for children. Arabinogalactans are long, dense polysaccharides with different molecular weights (10,000-120,000). Generally, lower molecular weight polysaccharides exhibit anti-inflammatory, anti-complement, anti-allergic effects, while higher molecular weight stimulatory effects are manifested by Natural Killer (NK) cell cytotoxicity and reticuloendothelial cells. Larch arabinogalactans are an excellent source of dietary fiber and are capable of producing short chain fatty acids (primarily butyrate) by vigorous fermentation of the gut flora. Butyrate is critical to normal colonic health because it is the preferred substrate for energy production by colonic epithelial cells, butyrate also acts as a protective agent for the intestinal mucosa against disease, arabinogalactans have been shown to reduce ammonia production and therefore may have clinical value in the treatment of portal systemic encephalopathy, a disease characterized by hepatic ammonia accumulation, and larch arabinogalactans are administered to human subjects resulting in increased levels of beneficial intestinal anaerobes, particularly bifidobacterium longum.
In the technical scheme, a small amount of water is added into defatted larch wood powder after extraction of larch arabinogalactan and the treatment is carried out at a static ultrahigh pressure (300-.
2. The coconut (scientific name: Cocos nucifera L.) is a plant of the genus Cocos of the family Palmae, which is tall and big in plant, tall and tall in tree, 15-30 meters in height, thick and strong in stem, provided with annular leaf marks, thick in base and often provided with fasciculated small roots. The thick petiole, the axil of the inflorescence, the egg-shaped or nearly spherical shape of the fruit, the cavity of the fruit containing the endosperm (i.e. "pulp" or kernel), the embryo and the juice (coconut water), and the flowering and flowering phases are mainly in autumn. Coconut is native to southeast Asia and the Pacific Islands, and is cultivated in the south of Guangdong, China and the tropical regions of the peninsula, Hainan, Taiwan and south Yunnan. Coconut juice and coconut meat contain a large amount of protein, fructose, glucose, sucrose, fat, vitamin B1, vitamin E, vitamin C, potassium, calcium, magnesium and the like, and also contain various trace elements. The coconut meat is white like jade, fragrant, smooth and crisp; coconut juice is cool and sweet. Coconut meat and coconut juice are delicious good fruits suitable for both the old and the young. Contains rich dietary fiber and almost no starch. Coconut meat is rich in coconut oil, is one of the healthier grease in the world at present, and has a plurality of health benefits and purposes. Coconut meat also contains abundant non-starch polysaccharide, namely dietary fiber.
Besides cellulose, other polysaccharides in coconut flour are arabinoxylogalactans, galactomannans, arabinomannans and galactoglucomannans. Mannan is the major reserve material in the endosperm of the seeds of the palmaceae family, also known colloquially as coconut meat. Mannan is an oligosaccharide consisting of several mannose or mannose linked to glucose by glycosidic bonds. Mannan has effects of reducing weight, moistening intestine, and regulating nutrition balance. Mannatide has wide application in the fields of relieving cancer, lung disease, gastropathy, diabetes treatment and diagnosis, etc. Mannan has certain immunogenicity, can stimulate the immune response of organisms, and can be used as an adjuvant of foreign antigens (such as certain toxins, viruses and fungal cells) to be combined with the surfaces of the foreign antigens, slow the absorption of the antigens and increase the titer of the antigens, thereby enhancing the cellular and humoral immune response of animals. Protecting the intestinal tract: mannan has good functions of identifying, adhering and removing intestinal pathogenic microorganisms, and can improve the growth performance and disease resistance of organisms mainly by regulating intestinal microflora, promoting intestinal growth and enhancing specific and nonspecific immunity. Mannan is an important intestinal function regulator, and can remarkably regulate the proliferation of advantageous flora such as beneficial flora in intestinal tract (such as bifidobacterium, lactobacillus casei, lactobacillus acidophilus and lactobacillus delbrueckii) and the proliferation of bifidobacterium and lactobacillus. But also promotes the fermentation of intestinal contents to generate acetic acid and lactic acid, so that the pH value of the intestinal tract is reduced, and the growth and the propagation of harmful bacteria such as escherichia coli, clostridium perfringens and the like are inhibited.
According to the technical scheme, the coconut powder is degreased by using an organic solvent, the degreased coconut powder basically does not contain grease, a 1000 Dalton hollow filter membrane is used for removing fructose, glucose, sucrose and other substances, and cellulose and other dietary fibers insoluble in water and macromolecules are retained. Spray drying pretreatment, and ultrahigh pressure homogenization (150 MPa) is adopted, so that the defatted coconut powder reaches a nanometer level and is easier to absorb.
3. Dietary fiber is a polysaccharide that is neither digested nor absorbed by the gastrointestinal tract, nor produces energy. Therefore, once considered a "nutrient-free" has not been adequately appreciated for long periods of time. With the intensive development of nutrition and related science, people gradually find that dietary fiber has quite important physiological effects. So that dietary fiber is more of concern to academia and common people today as the diet becomes more refined, and is recognized as a seventh group of nutrients by the nutritional community, in parallel with the traditional six groups of nutrients-protein, fat, carbohydrate, vitamin, mineral and water.
The dietary fiber has close relation with human health due to the unique physiological action, and the beneficial effect of the dietary fiber in the gastrointestinal tract can not be separated from the participation of the intestinal flora. The relationship between the microorganisms and the dietary fiber is explained in three aspects of the generation of the relationship between the microorganisms and the dietary fiber, the utilization of the dietary fiber by the intestinal microorganisms and the influence of the dietary fiber on the intestinal flora. With the deep understanding of the action mechanism between the two, it is possible to utilize different dietary fibers to produce predictable changes to intestinal micro-ecosystem, so that the intestinal micro-ecosystem can be used for more targeted human health. Dietary fibers with different intestinal flora metabolisms require different enzymes, and therefore the composition of dietary fibers has a dominant effect on the dynamic changes of the members of the intestinal flora. For example, some dietary fibers that can be fermented result in an increase in the number of bifidobacteria and lactobacilli, since these bacteria have a fermenting effect on carbohydrates, so that the bacteria are multiplied and increased in number, and inulin results in a reduction in the metabolites of harmful bacteria. The change of the composition of the intestinal flora is related to the composition of the dietary fiber, and the dietary fiber promotes the growth of probiotics and inhibits the growth of harmful bacteria by changing the composition of the intestinal flora. Thereby coordinating the balance of the bacterial system and the health of the microenvironment.
4. The larch arabinogalactan composition provided by the invention comprises larch arabinogalactan, defatted coconut powder, inulin and the like, and clinical tests of volunteers prove that the larch arabinogalactan composition has a good curative effect on diarrhea-type irritable bowel syndrome, and substitutes a drug therapy with a certain side effect in a food therapy mode.
5. The instantaneous pressure-increasing/reducing sterilization device can minimize the quality change of the sterilization object. According to the condition of instantaneous pressure and temperature change, the cell walls of bacteria and microorganisms are destroyed, so that the aim of sterilization is fulfilled. The sterilization time is short, the efficiency is high, the quality change of the arabinogalactan particles can be reduced to the minimum, bacteria, microorganisms and parasite eggs attached to the surfaces of the arabinogalactan particles can be effectively killed, and no side effect is caused to the environment and operators. The sterilization device adopts a Laval nozzle structure, and has simple structure and small device volume. The temperature in the pressurizing cabin is 80-85 ℃, and the pressure is 10 Mpa; the internal temperature of the decompression chamber is 18-23 ℃, and the pressure is 0.05 Mpa. The residence time of the arabinogalactan particles in the pressurizing chamber is 2s, and the residence time of the particles in the low-pressure chamber is 1 s. The driving gas for the powder particles in the chamber was dry air with a flow rate of 40 m/s. Can effectively kill bacteria, microorganisms and parasitic ova attached on the surfaces of the particles.
Drawings
FIG. 1 is a view of an instantaneous pressure-increasing/decreasing sterilization apparatus.
1. Air dryer, 2. blower; 3. the system comprises an electromagnetic valve, 4 parts of a pressurizing cabin, 5 parts of a temperature sensor, 6 parts of a temperature control pipe, 7 parts of a pressurizing pump and 8 parts of a feeding hopper; 9. a pressure sensor; 10. a straight pipe, 11, a decompression pump; 12. decompression chamber, 13, discharge port.
Detailed Description
The invention is described in more detail below with reference to specific examples, without limiting the scope of the invention. Unless otherwise specified, the experimental methods adopted by the invention are all conventional methods, and experimental equipment, materials, reagents and the like used in the experimental method can be obtained from commercial sources.
Example 1
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles, wherein the water content of the wood chip particles is 35 percent, treating 10kg of the wood chip particles by using 10 times of 95 percent ethanol, continuously stirring for 4 hours, standing for 10 hours, centrifugally filtering to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, treating for 15 minutes under 300 MPa, centrifugally filtering to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring for 2 hours, standing overnight, filtering to obtain filtrate, combining the filtrate for 2 times, concentrating to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65 percent, stirring for 2 hours, standing for 20 hours, removing supernatant, centrifuging to obtain precipitates, dissolving the precipitates by using hot water, cooling, adding ethanol again until the ethanol concentration of the solution reaches 60 percent, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.07, homogenizing under high pressure, and spray drying to obtain larch arabinogalactan. And (3) performing rapid sterilization treatment by using an instantaneous pressure-increasing and pressure-reducing sterilization treatment device to obtain 700g of arabinogalactan, wherein the water content is 3 percent, and the detected content of the arabinogalactan is 85.7 percent.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration of the coconut pulp slurry to reach 80%, placing the coconut pulp slurry in an ultrahigh pressure (400 MPa) device for processing for 15 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 daltons, collecting the intercepted part and the filter residue B for mixing, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure of 50, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder with the particle size of below 1 mu m.
S3, sterilizing a finished product;
3 parts of larch arabinogalactan prepared by S1, 0.4 part of defatted coconut powder prepared by S2 and 0.5 part of inulin are uniformly mixed, and are subjected to rapid sterilization treatment by an instantaneous pressure increase and decrease sterilization treatment device to obtain the larch arabinogalactan composition.
Example 2
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 350 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan 740g, arabinogalactan water content of 3%, and arabinogalactan content of 86.2% by detection.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
the preparation method of the defatted coconut powder comprises the following steps: cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration of the coconut pulp slurry to reach 80%, placing the coconut pulp slurry in an ultrahigh pressure (400 MPa) device for processing for 15 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 daltons, collecting the intercepted part and the filter residue B for mixing, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder with the particle size of below 1 mu m.
S3, sterilizing a finished product;
and (3) uniformly mixing 6 parts of larch arabinogalactan prepared in the step (S1), 3 parts of defatted coconut powder prepared in the step (S2) and 3 parts of inulin, and performing rapid sterilization by using an instantaneous pressure increase and decrease sterilization device to obtain the larch arabinogalactan composition.
Example 3
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 400 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan 800g with water content of 3% and arabinogalactan content of 86.8%.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration in the coconut pulp slurry to reach 85%, placing the slurry in an ultrahigh pressure device for processing for 10 minutes at 300 MPa, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the intercepted part and the filter residue B to be mixed, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure range of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder.
S3, sterilizing a finished product;
and (3) uniformly mixing 4 parts of larch arabinogalactan prepared in the step (S1), 3 parts of defatted coconut powder prepared in the step (S2) and 3 parts of inulin, and performing rapid sterilization by using an instantaneous pressure increase and decrease sterilization device to obtain the larch arabinogalactan composition.
Example 4
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 450 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.07, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan 860g with water content of 3% and arabinogalactan content of 85.9%.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring out coconut juice, taking out coconut meat, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration in the coconut meat slurry to reach 90%, placing the coconut meat slurry in an ultrahigh pressure device for 250 MPa for treatment for 20 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the intercepted part and the filter residue B for mixing, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure range of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder with the particle size of below 1 mu m.
S3, sterilizing a finished product;
5 parts of larch arabinogalactan prepared by S1, 3 parts of defatted coconut powder prepared by S2 and 3 parts of inulin are uniformly mixed, and are subjected to rapid sterilization treatment by an instantaneous pressure increase and decrease sterilization treatment device to obtain the larch arabinogalactan composition.
Example 5
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 500 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan, 928g arabinogalactan, water content of 3%, and detected arabinogalactan content of 86.7%.
The preparation method of the defatted coconut powder comprises the following steps: cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration of the coconut pulp slurry to reach 80%, placing the coconut pulp slurry in an ultrahigh pressure (400 MPa) device for processing for 15 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 daltons, collecting the intercepted part and the filter residue B for mixing, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder with the particle size of below 1 mu m.
S3, sterilizing a finished product;
and (3) uniformly mixing 3 parts of larch arabinogalactan prepared in the step S1, 1 part of defatted coconut powder prepared in the step S2 and 3 parts of inulin, and performing rapid sterilization by using an instantaneous pressure increase and decrease sterilization device to obtain the larch arabinogalactan composition I.
Example 6
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 550 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan 932g with water content of 3% and arabinogalactan content of 85.7%.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration in the coconut pulp slurry to reach 80%, placing the slurry in an ultrahigh pressure device for processing for 15 minutes at 100 MPa, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the intercepted part and the filter residue B to be mixed, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure range of 100 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder.
S3, sterilizing a finished product;
and (3) uniformly mixing 6 parts of larch arabinogalactan prepared in the step (S1), 2 parts of defatted coconut powder prepared in the step (S2) and 3 parts of inulin, and performing rapid sterilization by using an instantaneous pressure increase and decrease sterilization device to obtain the larch arabinogalactan composition.
Example 7
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 500 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 55%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan 900g, arabinogalactan water content of 3%, and arabinogalactan content of 95.7% by detection.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconuts, pouring out coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration in the coconut pulp slurry to reach 75%, placing the coconut pulp slurry in an ultrahigh pressure device for 50 MPa for treatment for 10 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the intercepted part and the filter residue B for mixing, adding appropriate amount of water to enable the concentration of dry matter of the liquid to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure range of 50 MPa, homogenizing for 30 minutes, and spray-drying to obtain the defatted coconut powder.
S3, sterilizing a finished product;
and (3) uniformly mixing 6 parts of larch arabinogalactan prepared in the step (S1), 1 part of defatted coconut powder prepared in the step (S2) and 1 part of inulin, and performing rapid sterilization by using an instantaneous pressure increase and decrease sterilization device to obtain the larch arabinogalactan composition.
Example 8
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by using a cutting machine, crushing the wood chips into small particles, wherein the water content of the wood chip particles is 35%, treating and soaking the wood chip particles by using 10 times of 95% ethanol, continuously stirring the wood chip particles for 4 hours, standing the wood chip particles for 10 hours, and performing centrifugal filtration to obtain filtrate 1 and filter residue 1; soaking the filter residue 1 in 10 times of organic solvent, stirring for 2 hours, filtering to obtain a filtrate 2 and a filter residue 2, and combining the filtrate 1 and the filtrate 2 for other purposes; adding 5 times of water into filter residue 2, soaking and stirring for 2 hours, standing overnight, carrying out centrifugal filtration to obtain a filtrate 3 and a filter residue 3, carrying out reduced pressure recovery on the filtrate 3 to obtain a filtrate 4, continuously putting the filter residue 3 into an ultrahigh pressure device by using 4 times of water, processing for 10 minutes by using 600 MPa, carrying out centrifugal filtration to obtain a filtrate 5, combining the filtrate 4 and the filtrate 5, concentrating to a certain volume, slowly adding ethanol to enable the ethanol concentration of the solution to reach 70%, stirring for 4 hours, standing for 20 hours, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate by using hot water, cooling, adding ethanol again to enable the ethanol concentration of the solution to reach 70%, stirring for 4 hours, standing for 20 hours, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate by adding water to enable the specific gravity of the solution to reach 1.07, carrying out high-pressure homogenization, spray drying, carrying out rapid sterilization by using an instantaneous pressure-increasing and reducing sterilization device, obtaining larch arabinogalactan.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration in the coconut pulp slurry to reach 90%, placing the slurry in an ultrahigh pressure device for processing for 20 minutes at 300 MPa, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the intercepted part and the filter residue B to be mixed, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure range of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder.
S3, sterilizing a finished product;
and (3) uniformly mixing 6 parts of larch arabinogalactan prepared in the step (S1), 2 parts of defatted coconut powder prepared in the step (S2) and 2 parts of inulin, and performing rapid sterilization by using an instantaneous pressure increase and decrease sterilization device to obtain the larch arabinogalactan composition.
Example 9
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 500 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.07, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan, 928g arabinogalactan, water content of 3%, and detected arabinogalactan content of 86.7%.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring out coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry with a colloid mill, adding ethanol to make the ethanol concentration of the coconut pulp slurry reach 80%, placing in an ultrahigh pressure (400 MPa) device for processing for 15 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, recovering ethanol from part of the filtrate A under reduced pressure to obtain oil-water mixed solution, separating oil from water with a high-speed centrifugal device, removing coconut oil part, passing the residual water solution C through a hollow filter membrane with the molecular weight of 1000 Dalton, collecting the trapped part and the filter residue B, mixing, adding appropriate amount of water to make the concentration of liquid dry matter reach 20%, placing in an ultrahigh pressure homogenizer at the pressure of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder with the particle size below 1 μm.
S3, sterilizing a finished product;
adding 3 parts of larch arabinogalactan prepared by S1 and 0.5 part of defatted coconut powder prepared by S2 into 1 part of mannan, uniformly mixing, and rapidly sterilizing by using an instantaneous pressure-increasing and pressure-decreasing sterilization device to obtain the larch arabinogalactan composition II.
Example 10
Preparation method of larch arabinogalactan composition
S1, extracting arabinogalactan from larch; the method comprises the following specific steps:
cleaning roots of cultivated larch growing for more than 35 years, cutting the roots into small wood chips by a cutting machine, crushing the wood chips into small particles with the water content of the wood chips being 35%, processing and soaking the wood chips by 10 times of 95% ethanol, continuously stirring the wood chips for 4 hours, standing the wood chips for 10 hours, centrifugally filtering the wood chips to obtain filter residues, removing ethanol in the filter residues, adding 4 times of water into the filter residues, placing the filter residues in an ultrahigh pressure device, processing the filter residues for 15 minutes under 500 MPa, centrifugally filtering the filter residues to obtain filtrate and filter residues, adding 6 times of water into the filter residues again, stirring the filter residues for 2 hours, standing the filter residues overnight, filtering the filter residues to obtain filtrate, combining the filtrate for 2 times, concentrating the filtrate to a certain volume, slowly adding ethanol into the solution until the ethanol concentration of the solution reaches 65%, stirring the filtrate for 2 hours, standing the filtrate for 20 hours, removing supernate, centrifuging the precipitate to obtain precipitate, dissolving the precipitate by hot water, cooling the precipitate, adding ethanol again to make the ethanol concentration of the solution reach 60%, stirring for 2 hr, standing for 20 hr, removing supernatant, centrifuging to obtain precipitate, dissolving the precipitate in water to make the specific gravity of the solution reach 1.15, homogenizing under high pressure, spray drying, and rapidly sterilizing with instantaneous pressure and pressure increasing and reducing sterilization device to obtain larch arabinogalactan, 928g arabinogalactan, water content of 3%, and detected arabinogalactan content of 86.7%.
S2, preparing defatted coconut powder; the method comprises the following specific steps:
cutting fresh coconut open, pouring coconut juice, taking out coconut pulp, removing impurities, cleaning with clear water, grinding into slurry by using a colloid mill, adding ethanol to enable the ethanol concentration of the coconut pulp slurry to reach 80%, placing the coconut pulp slurry in an ultrahigh pressure (400 MPa) device for processing for 15 minutes, taking out, centrifuging and filtering to obtain filtrate A and filter residue B, decompressing and recovering ethanol from part of the filtrate A to obtain oil-water mixed solution, separating oil from water by using a high-speed centrifugal device, removing the coconut oil part, enabling the residual water solution C to pass through a hollow filter membrane with the molecular weight of 1000 daltons, collecting the intercepted part and the filter residue B for mixing, adding appropriate amount of water to enable the concentration of liquid dry matter to be 20%, placing the mixture in an ultrahigh pressure homogenizer with the pressure of 150 MPa, homogenizing for 30 minutes, and spray drying to obtain defatted coconut powder with the granularity of below 1 mu m.
S3, sterilizing a finished product;
3 parts of larch arabinogalactan prepared by S1, 0.5 part of defatted coconut powder prepared by S2 and 1 part of resistant dextrin are added, the mixture is uniformly mixed, and the mixture is subjected to rapid sterilization treatment by an instantaneous pressure increase and decrease sterilization treatment device to obtain the larch arabinogalactan composition III.
Observation of the population treated with composition I of the pinus tabulaeformis arabinogalactan obtained in example 5 with diarrhea-predominant irritable bowel syndrome: a total of 40 volunteers were recruited and 4 were disabled. The effective and effective rate is 36 persons, the minimum age is 36 years, the maximum age is 79 years, wherein 22 persons are male, and 14 persons are female. The effect is obvious in the shortest time of 3 days, and the normal state is recovered in 10-15 days generally. The effective rate reaches 90 percent. The observation period is 2 months. The administration method is to take the samples prepared in example 5, 3 g each day before breakfast and supper.
The arabinogalactan composition I powder obtained in example 5 was sterilized by an instantaneous pressure increasing/reducing sterilization apparatus, and the sterilized powder was examined for the absence of bacteria, microorganisms, and parasite eggs.
The embodiments described above are merely preferred embodiments of the invention, rather than all possible embodiments of the invention. Any obvious modifications to the above would be obvious to those of ordinary skill in the art, but would not bring the invention so modified beyond the spirit and scope of the present invention.

Claims (5)

1. A sterilization treatment device for larch arabinogalactan composition is characterized in that the sterilization treatment device comprises a pressurizing cabin (4), a straight pipe (10), a decompression cabin (12), a blower (2) and an air dryer (1); an air outlet of the air dryer (1) is connected with an air inlet of the air blower (2), and an air outlet of the air blower (2) is connected with an air inlet of the pressurizing cabin (4); the pressurizing cabin (4) and the decompression cabin (12) are connected through a straight-flow pipe (10); a feeding hopper (8) is arranged at the upper part of the pressurizing cabin (4), and a discharge hole (13) is arranged at the bottom of the decompression cabin (12); temperature sensors and pressure sensors are arranged in the pressurizing cabin (4) and the decompression cabin (12), annular temperature control pipes (6) are arranged outside the pressurizing cabin (4) and the decompression cabin (12), and sealing electromagnetic valves are arranged at the joints of the pressurizing cabin (4) and the air blower (2), the feeding hopper (8) and the direct current pipe (10); the joints of the decompression chamber (12) and the straight-flow pipe (10) and the discharge hole (13) are provided with sealed electromagnetic valves, and the pressure sensor (9) in the pressurizing chamber (4) is connected with the pressurizing pump (7); the pressure sensor inside the decompression chamber (12) is connected to the decompression pump (11).
2. The process of the sterilization apparatus according to claim 1, wherein the process of the sterilization apparatus with instantaneous pressure increase and decrease comprises:
air A is changed into dry air through an air dryer (1), the dry air enters an air blower (2), then an electromagnetic valve (3) between a connecting pipe of the air blower (2) and a pressurizing cabin (4) is opened, and the dry air enters the pressurizing cabin (4);
b, opening an electromagnetic valve at a connecting pipe of the feeding hopper (8) and the pressurizing cabin (4), and allowing the larch arabinogalactan particles to enter the pressurizing cabin (4) from the feeding hopper (8);
c, regulating and controlling the temperature and pressure settings of a cabin of the pressurizing cabin (4) and a cabin of the decompression cabin (12), wherein a constant temperature value in the cabin is implemented by a temperature sensor (5) and a temperature control pipe (6) laid on the outer surface of the cabin, and a constant pressure value in the cabin is implemented by a pressure sensor, (9) a booster pump (7) and a decompression pump (11);
d, when the temperature and the pressure of the cabins of the pressure increasing cabin (4) and the decompression cabin (12) reach preset values, opening a direct current pipe (10) electromagnetic valve connected between the pressure increasing cabin (4) and the decompression cabin (12), and allowing the dry air and the larch arabinogalactan particles to pass through the direct current pipe (10) at a high flow rate and enter the decompression cabin (12) from the pressure increasing cabin (4);
e the particles subjected to the transient variation of temperature and pressure, i.e. the sterilized particles, are discharged from the outlet (13) of the decompression chamber (12).
3. The process of sterilizing a treatment device according to claim 2, wherein the inside temperature of the pressurizing chamber (4) in the process is 80 to 85 ℃ and the pressure is 10 Mpa; the internal temperature of the decompression chamber (12) is 18-23 ℃, and the pressure is 0.05 Mpa; the residence time of larch arabinogalactan particles in the pressurizing chamber (4) is 2s, and the residence time of particles in the decompression chamber (12) is 1 s; the driving gas for the particles in the chamber of the pressure increasing chamber (4) and the chamber of the decompression chamber (12) is dry air with the flow velocity of 40 m/s.
4. The sterilization apparatus as defined in claim 1, wherein the connection mode between each component of the sterilization apparatus is a space rigid closed type sealed pipe connection.
5. The sterilization apparatus as defined in claim 1, wherein said straight pipe (10) has a length of 0.8m and is externally coated with a heat insulating layer.
CN202210108197.5A 2019-07-09 2019-07-09 Preparation method of larch arabinogalactan composition and sterilization treatment device thereof Withdrawn CN114366826A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210108197.5A CN114366826A (en) 2019-07-09 2019-07-09 Preparation method of larch arabinogalactan composition and sterilization treatment device thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202210108197.5A CN114366826A (en) 2019-07-09 2019-07-09 Preparation method of larch arabinogalactan composition and sterilization treatment device thereof
CN201910614589.7A CN110251530A (en) 2019-07-09 2019-07-09 A kind of preparation method and its clinical application of larch arabinogalactan composition

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201910614589.7A Division CN110251530A (en) 2019-07-09 2019-07-09 A kind of preparation method and its clinical application of larch arabinogalactan composition

Publications (1)

Publication Number Publication Date
CN114366826A true CN114366826A (en) 2022-04-19

Family

ID=67925173

Family Applications (3)

Application Number Title Priority Date Filing Date
CN202210108197.5A Withdrawn CN114366826A (en) 2019-07-09 2019-07-09 Preparation method of larch arabinogalactan composition and sterilization treatment device thereof
CN202210108191.8A Withdrawn CN114377160A (en) 2019-07-09 2019-07-09 Preparation method of larch arabinogalactan composition
CN201910614589.7A Pending CN110251530A (en) 2019-07-09 2019-07-09 A kind of preparation method and its clinical application of larch arabinogalactan composition

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN202210108191.8A Withdrawn CN114377160A (en) 2019-07-09 2019-07-09 Preparation method of larch arabinogalactan composition
CN201910614589.7A Pending CN110251530A (en) 2019-07-09 2019-07-09 A kind of preparation method and its clinical application of larch arabinogalactan composition

Country Status (1)

Country Link
CN (3) CN114366826A (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112871077A (en) * 2020-12-29 2021-06-01 池州天赐高新材料有限公司 Process for granulating 3,3 '-dichloro-4, 4' -diaminodiphenylmethane in small granules
CN113907239B (en) 2021-12-14 2022-03-22 中国农业科学院农产品加工研究所 Continuous powder steam pressure difference sterilization system and sterilization method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010012567A (en) * 2008-05-26 2011-02-22 Fujiwara Techno Art Co Ltd Method for sterilization of powdery or granular substances and apparatus for the sterilization by the method.
CA2777941C (en) * 2009-11-12 2018-08-14 Nestec S.A. Nutritional composition for promoting gut microbiota balance and health
CN109432242A (en) * 2018-11-07 2019-03-08 郑毅男 A kind of larch arabinogalactan preparation method and its application in terms of medical treatment

Also Published As

Publication number Publication date
CN110251530A (en) 2019-09-20
CN114377160A (en) 2022-04-22

Similar Documents

Publication Publication Date Title
CN104432084A (en) Conditioning powder and preparation method and application thereof
Verma et al. Psyllium (Plantago ovata) husk: a wonder food for good health
KR101339706B1 (en) A compound for immune strengthen inclusion reducing the bitterness of red ginseng, the extract of immune, and the probiotics
JP6404339B2 (en) Composition having function of alleviating premenstrual syndrome and menstrual pain
CN106942748A (en) A kind of female acyesis tailored version clinical nutrition formula and preparation method thereof
CN106942542A (en) A kind of cubilose beverage of the grain of suspension containing nostoc and preparation method thereof
CN106265763A (en) There is slow down aging and the compositions of skin-care functional and application thereof
CN107746435A (en) Polysaccharide from Portulaca oleracea extract and its production and use
CN114366826A (en) Preparation method of larch arabinogalactan composition and sterilization treatment device thereof
CN101518335B (en) Pectin containing insoluble dietary fiber, preparation method and application thereof
KR20070100560A (en) Functional drink for immune- enhancing and manufacturing method thereof
CN111328948A (en) Ginger paste and preparation method thereof
CN111387394A (en) Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
CN103920140A (en) Compound preparation for reducing blood glucose, body weight and fat for human
CN113925172A (en) Giant salamander peptide nutritional composition suitable for children
CN114246222A (en) Plant-based yoghourt with functions of calming nerves, helping sleep and improving gastrointestinal tract and preparation method thereof
WO2014134830A1 (en) Edible composition, food product comprising same, and preparation method for the food product
CN107375697B (en) Compound preparation with bowel relaxing function and preparation method and application thereof
CN112042846A (en) Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
CN101194714B (en) Brain tonic nutrient solution for children
KR20220096379A (en) Method for preparing antler extract and kyung-ok-go containing antler extract
CN107518357A (en) A kind of enhancing immune composition containing Effects of Extracts of Grifola frondosa on Active and preparation method thereof
CN107412283A (en) A kind of three high drop composition containing Agricus blazei extract and preparation method thereof
CN107373259A (en) One kind relaxes bowel Chinese herbal health-caring beverage and preparation method thereof
CN105770474A (en) Lupus erythematosus resisting drug for combined immunization and preparing method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20220419