CN109432242A - A kind of larch arabinogalactan preparation method and its application in terms of medical treatment - Google Patents
A kind of larch arabinogalactan preparation method and its application in terms of medical treatment Download PDFInfo
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- CN109432242A CN109432242A CN201811317388.2A CN201811317388A CN109432242A CN 109432242 A CN109432242 A CN 109432242A CN 201811317388 A CN201811317388 A CN 201811317388A CN 109432242 A CN109432242 A CN 109432242A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/68—Plantaginaceae (Plantain Family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/10—Laxatives
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
Abstract
Pine stump wood dust of falling leaves is broken, with alcohol treatment soaked overnight, ultra high pressure treatment 20 minutes, filtering, removes filtrate, and residue is impregnated twice with water, merge 2 filtrates, concentration, using macroreticular resin as chromatographic material, is eluted with water, the excessively hollow filter membrane of eluent, filtrate is discarded, retention part is larch polysaccharide, is spray-dried to obtain larch arabinogalactan after homogeneous.Larch arabinogalactan is combined with Ampelopsis grossedentata extrat, is increased the solubility of dihydromyricetin in water, is improved bioavilability.
Description
Technical field
The present invention relates to the compatibility of larch arabinogalactan composition and preparation method is extracted, belongs to biotechnology
Field.
Background technique
The main source of arabogalactan is fallen leaves pine tree (Larix sp.), although larch arabinogalactan
Sugar extraction-western larch (Larix occidentalis) or Mongolian larch (Larix from two kinds of sources
), dahurica but most of larches can produce arabogalactan.Larch arabinogalactan is a kind of dry
Dry, the powder of free-flowing has very slight rosin smell and sweet taste.It is 100% water-soluble and be low viscosity
Solution.Due to its excellent dissolubility and mild taste, powder is readily mixed into water and fruit juice and conveniently takes.
Larch arabinogalactan (Arabinogalactans) is the polysaccharide of a kind of length and multi-branched.In nature
In, arabogalactan is present in several microorganism systems, and in especially acidproof mycobacteria, it is in peptide glycan and mould
A kind of compounding ingredients between bacterium acid as cell wall, and influence the immune response of monocyte macrophage tuberculosis antigen
Property.Many plants arabogalactan all rich in, it is most of with glycoprotein forms combination threonine, proline or silk
The protein backbone of propylhomoserin (" Arabinogalactan-Protein ").These plants include leek seed, carrot, radish, semen sojae atricolor,
Pears, corn, wheat, red wine, Italian ryegrass, tomato, artemisiifolia, sorghum, bamboo is careless, in coconut meat and milk.Many grass
Medical instrument has good Immune-enhancing effect performance, such as Echinacea, western arborvitae, Radix Angelicae Sinensis and turmeric, wherein all containing a large amount of Arabic half
Newborn glycan.All arabogalactans separated from larch are the water-soluble nitrogen-free polysaccharide of 3,6- β-D- galactan sugar-type.
Experimental analysis determines that larch arabinogalactan is the hyperbranched chemoattractant molecule with 3,6- galactan main chain.Galactolipin
Molar ratio with arabinose unit (by β-galactopyranose, β-arabinofuranose and β-arabopyranose) is about 6:1,
It and include that total carbohydrate content is greater than 99%.Micro glucuronic acid is typically also very common.Advanced larch Arabic half
Newborn glycan (commonly used in the grade of clinical application) is made of the arabogalactan greater than 98%.
The pharmacokinetics of larch arabinogalactan: the medicine of larch arabinogalactan under oral dose
It is not yet illustrated well in human body for dynamics.Larch arabinogalactan is absorbed after oral doses
Absolute concentration is unclear, however, unabsorbed larch arabinogalactan is by gastrointestinal microorganisms flora boiling fermentation.
In animal model, after injection, larch arabinogalactan is removed from blood, half-life period is 3.8 minutes.
90 minutes after injection, concentration highest in liver (52.5%) and urine (30%), hepatic clearance after first order kinetics and
Half-life period is 3.42 days.The known purifying arabogalactan from western larch be it is known in vitro and liver is without saliva
Sour glycoprotein receptor combines.Experiment in vivo evidence also demonstrates this property in conjunction with liver asialoglycoprotein receptor.
The arabogalactan for reaching liver by portal circulation is rapidly and special in liver cell by receptor-mediated encytosis
Opposite sex internalization.Since the ratio that larch arabinogalactan reaches liver is very high, and it absorbs the active of liver cell,
Groman et al. thinks that arabogalactan may be the ideal carrier to hepatic delivery drug.
Evidence is also shown that the mankind eat larch arabinogalactan to enhancing intestinal microflora, especially increases
Add anaerobic bacteria such as Bifidobacterium and Bacillus acidi lactici, while reducing clostridium with remarkable effect.Pass through enteric microorganism
Carbohydrate fermentation at short chain fatty acids (SCFAs), is presently believed to most important to big gut function and possible by fauna
Be liver and peripheral tissues metabolism facilitate factor.Butyric acid plays an important role in colon health, it is colon epithelial cell
Generate the preferred substrate of energy, it is believed that it can protect mucous membrane and resists a variety of intestines problems, and have shown that they can be protected
These cells are protected from the influence of cancer promotor.The ability that arabogalactan increases butyric acid salinity show to take orally to
After medicine, some significant health benefits are arabogalactans by intestinal microflora boiling fermentation, cause to generate
SCFAs.The high yield of SCFA shows the Arabic gala that ferments almost exclusively through the intestinal microflora of no any absorption
Glycan is transported by large intestine.In a research of Vince et al., external coproculture system research enteric bacteria has been used
Metabolism to complex carbohydrate.Lactulose, pectin, arabogalactan is added in human faecal mass homogenate and anaerobic culture medium
And cellulose, before and after subject's preparatory every kind of carbohydrate of feeding.The fermentation of the substrate of addition passes through
It generates short chain fatty acids and inhibits net ammonia generation to assess being incubated in a few hours, which reports that arabogalactan is in office
When the yield that all can improve in all samples SCFAs and acetic acid is waited, and the concentration of butyric acid is more than propionic acid in all samples.With it is right
Compare according to group, does not show more SCFA yield with the excrement homogenate that cellulose is cultivated together.Englyst et al. has studied
Decomposition of the human feces bacterium to larch arabinogalactan and other three kinds of polysaccharide, observation is not in homopolysaccharide substrate
The generation of SCFAs.They have found that skeleton and side chain sugar arabogalactan collectively serve as fermentation substrate, however, these are sugared
It is obtained than starch or pectin slower.Their experiment shows that arabogalactan especially has in terms of increasing propionic acid salinity
Benefit.Although starch is the most effective polysaccharide for generating high butyric acid density, arabogalactan compares pectin or xylan, tool
There is considerably higher butyric acid to generate.The subject for giving arabogalactan in advance reduces ammonia and generates, however, for all meals
For eating fiber, this effect is not common, because cellulose has been demonstrated that the generation on ammonia does not influence.Vince et al. is mentioned
Arabogalactan has medical value because this ability reduces the generation of ammonia and subsequent absorption out.Ah
Draw primary galactan that can promote the increase of Bifidobacterium, especially bifidobacterium longum, this seems to fermentation arabinogalactan
Sugar has maximum specificity.Carried out in human volunteer the comparative study to larch arabinogalactan replenishers it
Afterwards, the evidence that do not deliver again shows that the increase that can promote Bifidobacterium and other anaerobic bacterias such as Bacillus acidi lactici.Perhaps this
It is the largest potentiality polysaccharide, is a kind of biological respinse to support traditional treatment of cancer.The composite attribute of its Immune-enhancing effect and
Its antimetastatic activity meets the target needed in two kinds of treatments.Although modified citrus pectin in tradition and substitute all by
It pays close attention to, the antimetastatic activity delivered on alternative medicine periodical, the effect of larch arabinogalactan is basically the same
Mode passes through the galactosylated acceptor with liver competitive binding liver organization by inhibiting metastatic cells to be attached to liver；However,
Larch arabinogalactan also provides the advantage of additional enhancing immune function.
Although the clinical application research of this novel polysaccharide not yet carried out so far, it is wide that physiological activity shows that it has
General potential therapeutic effect.As dietary fiber replenishers, larch arabinogalactan have the function of it is several beneficial,
Ability including promoting friendly bacterium growth, increases the yield of SCFA, and reduce the generation of ammonia.Since most of diet lack meals
Fiber is eaten, immunity is not only adjustable, but also improve fiber intake, shows with double action, this unique polysaccharide is not
It is ashamed to be a kind of advantage dietary fiber.
Larch arabinogalactan is used for medicine, and larch arabinogalactan is used to infect, including flu, stream
Sense, H1N1(pig) influenza, children's ear infection and AIDS virus/AIDS.It is also used for treatment liver cancer, and by hepatic injury
Cerebral disorders caused by (hepatic encephalopathy).Also someone is used as dietary fiber, reduces cholesterol, and enhance immune system.Larch
Food of the arabogalactan as friendly enteric bacteria, is exactly " prebiotics " described in us, therefore, when it is consumed,
It generates positive influence to the micropopulation in enteron aisle.It realizes this point by providing the substance of microbial flora fermentation,
So as to cause generation short chain fatty acids (SCFA), such as butyrate and propionate.These SCFA are most important to the health of colon,
Enough short chain fatty acids potentially contribute to prevent the development of inflammatory bowel disease and colon cancer.
A method of extracting dihydroquercetin and arabogalactan from larch and its industrial wood waste
(CN200710144650.3) it points out, the method for dihydroquercetin is extracted from larch and its industrial wood waste: crushing, hot water
It impregnates, low-carbon alcohols are added in soak, are allowed to precipitate, gained sediment is exactly arabogalactan, is both in low-carbon alcohol solution
The dihydroquercetin to be extracted.We have found that hot water soluble a variety of impurity, can be brought into Arabic half by the deficiency of this method
In newborn glycan, to increase the purifying procedure and difficulty of arabogalactan.It falls leaves if handled in advance with low-carbon alcohol solution
Loose powder end not only can be reduced the introducing of impurity in arabogalactan extraction process, but also can increase the receipts of dihydroquercetin
Rate.A kind of extracting method of larch arabinogalactan (104961841 A of CN), this method disclose: larch wood chip powder
Broken, with petroleum ether soak degreasing, degreasing larch wood powder extracts me with chlorination 1- butyl -3- methylimidazole ionic liquid solution
Primary galactan, obtains extracting solution, and extracting solution is added in ethanol solution and obtains sediment, sediment is filtered, filtration cakes torrefaction is
Obtain larch arabinogalactan.This method pre-processes larch powder using organic solvent, improves
Increase the introducing of arabogalactan impurity in CN200710144650.3 patent with hot water treatment first.But it then uses
Organic solvent extracts arabogalactan, extracts without the use of water, this not only increases cost, also without more environmentally friendly with water.
Arabogalactan is by providing main energy sources for colon cell and increasing beneficial bacteria such as Bifidobacterium
Protect enteron aisle from the influence of disease and cancer promotor with lactic acid bacteria.Larch arabinogalactan substantially balances
A kind of good method of flora protection gastrointestinal tract.Since most of immune system resides in gastrointestinal tract, this be larch I
Primary galactan can enhance one of mechanism of immune system.The another way of this marvellous compound enhancing immune system
It is by macrophage and natural kill (NK) cell-stimulating.Pass through the immune system components for stimulating both important, larch
Arabogalactan can directly affect human body target direction bacterium and virus and the outside intruders such as cancer or precancerous cell
Ability.It has been displayed to promote with chronic fatigue syndrome, virus hepatitis, the trouble of HIV/AIDS and autoimmune disease
NK activity aspect in person is particularly useful.
Larch arabinogalactan eighties of last century is just approved by the FDA in the United States as dietary supplements, it is considered to be safety
Effective immunostimulation tonic.Due to its influence to gastrointestinal micro-flora, unique side effect be slight abdominal distension and
Ventosity, but these side effects be usually it is of short duration, only a minority experienced.Enteron aisle can be improved by, which taking daily, is good for
Health, or strengthen immunity is taken during acute illness.Its taste is mild, has excellent dissolubility in water and fruit juice,
Become the fabulous immune replenishers of children and adult.
Larch arabinogalactan safety: when as food consumption, larch arabinogalactan is safety
's.It may cause certain side effect, such as abdominal distension and intestines gas (flatulence).
Dihydromyricetin is from ampelopsis grossdentata (Ampelopsis grossedentata (Hand-Mazz)
W.T.wang isolated in).It is with very strong antioxidant activity, but its solubility in water: 0.84g/L
(20 DEG C), directly affect its bioavilability.
Mechanochemistry processing may result in many physical chemistry conversion of arabogalactan macromolecular, be valence first
Key is broken and is formed, secondly, being weak intramolecular interaction (unordered, allosteric rearrangement etc.).It is all these in turn again can
To change the bioactivity and toxicologic properties of polysaccharide.This patent using mechanochemistry processing larch arabinogalactan with
The combination of dihydromyricetin increases dihydromyricetin and exists to improve the anti-oxidant and other biological activities of arabogalactan
Solubility in water, increases the bioactivity of dihydromyricetin, to increase the life of larch arabinogalactan composition
Object availability.
Summary of the invention
1 method that the invention discloses a kind of to extract arabogalactan from larch, concrete operations are as follows: falling
Leaf pine stump sawdust is ground into the following little particle of diameter 0.5mm, first uses alcohol treatment soaked overnight, super-pressure (50 megapascal) processing
20 minutes, filtering removed filtrate, obtains filter residue；Filter residue adds 10 times of amount water soaked overnights again, filters to get filtrate, residue continues with 8 times
It measures water to impregnate 8 hours, filters to get filtrate, merge 2 filtrates, ethyl alcohol is recovered under reduced pressure, be concentrated into certain volume, centrifugation removal is insoluble
Substance obtains concentrate, using macroreticular resin as chromatographic material, concentrate loading is eluted with water after adsorbing 4h, collects eluent,
The hollow filter membrane for crossing 10,000 dalton of molecular weight discards filtrate, and retention part is larch polysaccharide, equal using super-pressure
Matter is spray-dried to obtain larch arabinogalactan.
2 are added appropriate Ampelopsis grossedentata extrat (mainly containing dihydromyricetin) water tune specific gravity to 1.08-1.12, using
Super-high-pressure homogenization is spray-dried to obtain larch arabinogalactan composition.
3. larch arabinogalactan is matched with other dietary fibers, the medical value of its own is improved.
Have the active effect that 1) ultra high pressure extraction reduces hot water extraction process, reduces energy consumption, while reducing heating
The introducing of impurity in the process simplifies purifying procedure；Addition Ampelopsis grossedentata extrat considerably increases larch arabinogalactan
Antioxidation acts on clearance rate 62.51%, larch to DPPH radicals scavenging by simple larch arabinogalactan
Arabinogalactan composition is increased to 86.54%；Arabogalactan passes through at mechanochemistry together with dihydromyricetin
Reason increases the solubility of dihydromyricetin in water by several times, is increased to 3.38g/L by original 0.84g/L.It is fallen to increase
The bioavilability of leaf pine arabogalactan dihydromyricetin promotor composition.Larch arabinogalactan and other diets
Fiber compatibility is effectively improved constipation symptom, and constipation crowd observation experiment is as a result, efficient up to 85%.
Phend-sulphuric acid measures larch polyoses content
One) instrument and reagent
Reagent: one water (C of glucose6H12O6·H2O, MW=198) it is that analysis is pure, it is limited purchased from Chinese medicines group chemical reagent
Company；Phenol is that analysis is pure, is purchased from Jinan Xiao Shi Chemical Co., Ltd.；Sulfuric acid is that analysis is pure, is had purchased from Laiyang City Kant's chemical industry
Limit company.Sample to be tested pine tree polyoses extract is provided by teacher Zheng Yinan.
Instrument: 8302 type water-baths (mayor of Beijing bearing instruments and meters company)；KQ5200E type ultrasonic cleaner (Kunshan
Ultrasonic instrument Co., Ltd, city).
Two), experimental procedure
1. the preparation of Glucose standards stock solution
Precision weighs C6H12O6·H2O 0.0102g, constant volume shakes up, obtains in 100 mL volumetric flasks after being dissolved with distilled water
Concentration is 0.0927mg/mL Glucose standards stock solution, is placed in spare in refrigerator.
2. 5%(g/mL) preparation of phenol solution
Phenol 5g is weighed, for constant volume in 100 mL brown volumetric flasks, obtaining mass fraction is 5%(g/mL after being dissolved with distilled water)
Phenol solution, be placed in spare in refrigerator.
3. the determination of maximum absorption wavelength
Precision measures 0.0927mg/mL Glucose standards stock solution 2mL and is placed in 10mL band plug teat glass, to band plug glass examination
In pipe be added 1.0mL 5% phenol solution, on turbula shaker mix after, it is adherent slowly, dropwise at the uniform velocity be added the dense sulphur of 5mL
Acid solution places 10min, sets in 40 DEG C of water-baths and keep the temperature 15min in mixing well on turbula shaker, takes out, rapid with ice water
It is cooled to room temperature.Same processing is carried out as blank control group using 2 mL distilled water, is scanned in 470 ~ 510nm, with determination
Maximum absorption wavelength.
After the aqueous solution of sample to be tested pine tree polyoses extract is reacted according to the above method, length scanning is carried out.
4. the drafting of glucose standard curve
Respectively it is accurate measure 0.0927 mg/mL Glucose standards stock solution 0mL, 0.2mL, 0.4mL, 0.6mL, 0.8mL,
1.0mL, 1.2mL are placed in 10mL band plug teat glass, are mended with distilled water to 2.0mL, are added respectively into band plug teat glass
The phenol solution of 1.0mL 5%, after mixing on turbula shaker, adherent respectively the at the uniform velocity addition 5mL concentrated sulfuric acid is molten slowly, dropwise
Liquid mixes well, and places 10min, sets in 40 DEG C of water-baths and keep the temperature 15min, takes out, is cooled down rapidly with frozen water to room temperature.To carry out
The distilled water equally handled is blank control group, in wavelength 489nm, measures absorbance value.With glucose content (μ g) for horizontal seat
Mark draws glucose standard curve using absorbance value as ordinate.
5. the preparation of testing sample solution
Precision weighs 4 parts of sample to be tested, and distilled water is added to dissolve, and is settled to 100 mL volumetric flasks, and obtaining concentration is 0.1 mg/mL's
Testing sample solution.
6. determination of polysaccharide in testing sample solution
Accurate above-mentioned 1 mL of testing sample solution of measurement is placed in 10mL band plug teat glass respectively, and water is added to mend to 2.0mL, accurate
5% phenol solution 1mL is added, shakes up, it is adherent slowly, dropwise at the uniform velocity be added concentrated sulfuric acid 5mL, shake up, place 10min, set 40 DEG C of water
15min is kept the temperature in bath, is taken out, ice water is rapidly cooled to room temperature, using the distilled water equally handled as blank, in wavelength
489nm measures absorbance.
Three) experimental result
1. the determination of maximum absorption wavelength
After measured, molten after dextrose standard sample stock solution (figure left) and testing sample solution (figure is right) and phenol and sulfuric acid reaction
Liquid is pink, has absorption maximum at 489 nm of wavelength, therefore selects 489 nm as measurement wavelength.
2. the drafting of glucose standard curve
3. determination of polysaccharide in sample to be tested pine extract
It is measured by standard curve (or external standard two-point method) and (is seen annex Excel table in detail)
Experimental example 2 closes object antioxidation
Experimental method
The preparation of 1.1 sample solutions
The larch arabinogalactan of Example 2 and 3 dihydromyricetin promotor composition 1g respectively is returned with 10 times of 70% ethyl alcohol of amount
Stream extracts 1 hour, extracting solution centrifugal filtration, and certain volume is concentrated, adds second alcohol and water that solution ethanol concentration is made to reach 70%, constant volume
Into 50ml volumetric flask, for examination.
1.2 larch arabinogalactan compositions act on DPPH radicals scavenging
The experiment of DPPH radicals scavenging effect is most common, because it is simple with antioxidant, directly, is rapidly reacted.
It weighs 0.0128 g of DPPH and is dissolved in it surely in 50 mL volumetric flasks with dehydrated alcohol, 10mL solution is then taken to be dissolved in 50 mL surely
It in volumetric flask, is protected from light, ultimate density is 1.3 × 10-4 mol/L。
With reference to the method for Luo etc., and make it is suitably modified, 96 orifice plates be used to quickly measurement sample absorbance value.It inhales respectively
Take 80 μ L of sample and positive reference substance prepare liquid, be added 80 μ L of DPPH solution, at 37 DEG C it is dark stand 30 minutes after, with nothing
Water-ethanol zeroing, measure 517nm wavelength at absorbance (ASample), each sample is measured in parallel 3 times；Take sample prepare liquid 80
μ L and 70% ethyl alcohol, 80 μ L mixed liquor at 517nm absorbance (A 1 ), then measure 80 μ LDPPH solution and 80 μ L70% ethyl alcohol
At 517nm wavelength absorbance (A 0 ).DPPH clearance rate is calculated by following formula.
DPPH clearance rate/%=(1-) × 100 (3.1)
As a result: simple larch arabinogalactan, to DPPH radicals scavenging exercising result: clearance rate 62.51%.
The larch arabinogalactan vine tea composition of 2 method of Example preparation, to DPPH radicals scavenging exercising result: clear
Except rate is 86.54%.
Embodiment 1
Pine stump of falling leaves is cleaned, and sawdust is ground into the following little particle of diameter 0.5mm, takes 100 kilograms, is first impregnated and is soaked with alcohol treatment
Overnight, super-pressure (400 megapascal) is handled 10 minutes bubble, and filtering removes filtrate, obtains filter residue；Residue adds 10 times of amount water to impregnate again
Night filters to get filtrate, and residue continues to be impregnated 8 hours with 8 times of amount water, filters to get filtrate, merges 2 filtrates, be concentrated under reduced pressure into one
Determine volume, centrifugation removal insoluble matter obtains concentrate, using macroreticular resin as chromatographic material, by concentrate loading, after adsorbing 4h,
It is eluted with water, collects eluent, cross the hollow filter membrane of 10,000 dalton of molecular weight, discard filtrate, retention part is larch
Polysaccharide is spray-dried to obtain larch arabinogalactan using super-high-pressure homogenization.Obtain 10kg larch arabinogalactan
Sugar.
It is 95.98% through detection polyoses content.
Embodiment 2
The larch arabinogalactan prepared by 1 method of embodiment is taken, by 2 method of experimental example
Antioxidation activity in vitro measurement is carried out, to DPPH radicals scavenging exercising result: clearance rate 62.51%.
Embodiment 3
1 method of Example preparation larch arabinogalactan 90g be added Ampelopsis grossedentata extrat (dihydromyricetin 90% with
On) 10g, larch arabinogalactan is spray-dried to obtain using super-high-pressure homogenization with water tune specific gravity to 1.08-1.12
Composition.The composition 10g is taken to add water 100ml, wherein dihydromyricetin solubility reaches 3.38g/L, dissolution in water
Degree obviously increases.To DPPH radicals scavenging exercising result: clearance rate 86.54%.
Experimental example 4
The larch arabinogalactan 65g of Example 1, is added resistant dextrin 17g, and circle bud isapgol husk 18g is placed in mixed
In even, mix, every bag of 6g, encapsulating.
Experimental example 5
The larch arabinogalactan 80g of Example 1 is added resistant dextrin 20g, is placed in vortex mixer, mixes, every bag
6g, encapsulating.
Experimental example 6
The larch arabinogalactan 80g of Example 1 adds the luxuriant isapgol husk powder 20g of circle, is placed in vortex mixer, mixes
It is even, every bag of 6g, encapsulating.
Experimental example 7
It is observed using the composition treatment constipation crowd in embodiment 4: recruiting volunteer and amount to 29 people, wherein 2 people that fall off, in vain
4 people.23 people are effectively significantly counted with effect, the age is 7 years old the smallest, and maximum 80 years old, wherein 12 people of male, 11 people of women.Most
That took effect within the short time 2 days has 2 people, generally restores normal at 10-15 days.Effective percentage 85%.The observation period one month.Instructions of taking:
The sample of 4 method of Example preparation, respectively takes 1 gram before meals sooner or later daily.
Embodiment 8
It is observed using the composition treatment constipation crowd in embodiment 4: recruiting volunteer and amount to 40 people, give be divided into four respectively
Group, every group of 10 people, every group of dosage are respectively 0.25g/ days, 0.5g/ days, 1.0g/ days, 3.0g/ days.It was found that its minimum has
Imitating dosage is 0.5g/ days.
Claims (5)
1. the present invention discloses a kind of preparation method with the larch arabogalactan composition for capableing of quick curing constipation,
It is characterized in that larch arabinogalactan composition mainly includes that 3-6 parts of larch arabinogalactan, vine tea mention
2-3 parts of object, 0.5-5 parts of semen plantaginis, resistance is taken to paste 0.2-3 parts.
2. larch arabogalactan composition according to claim 1, which is characterized in that larch Arab gala
Glycan the preparation method is as follows: fallen leaves pine stump clean, sawdust is ground into the following little particle of diameter 0.5mm, takes 100 kilograms, first uses
Alcohol treatment impregnates soaked overnight, and super-pressure (50 megapascal) is handled 20 minutes, and filtering removes filtrate, obtains filter residue；Residue adds 10 again
Amount water soaked overnight again, filters to get filtrate, and residue continues to be impregnated 8 hours with 8 times of amount water, filters to get filtrate, merges 2 filtrates,
Ethyl alcohol is recovered under reduced pressure, is concentrated into certain volume, centrifugation removal insoluble matter obtains concentrate, will using macroreticular resin as chromatographic material
Concentrate loading is eluted with water after adsorbing 4h, collects eluent, crosses the hollow filter membrane of 10,000 dalton of molecular weight, discards filter
Liquid, retention part are spray-dried to obtain larch arabinogalactan using super-high-pressure homogenization for larch polysaccharide.
3. larch arabogalactan composition according to claim 1, which is characterized in that the dosage for treating constipation is
0.5-3g/ days.
4. according to preparation method described in right 2, which is characterized in that the larch arabinogalactan being prepared is added suitable
The Ampelopsis grossedentata extrat containing 90% or more dihydromyricetin is measured, it is spraying using super-high-pressure homogenization with water tune specific gravity to 1.08-1.12
Dry larch arabinogalactan composition.
5. the larch arabinogalactan composition being prepared into according to claim 3, which is characterized in that dihydromyricetin water
In solubility obviously increase, reach 3.38g/L.
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