CN107135792A - It is a kind of to be used for the method for restoration of the ecosystem to tooth moss - Google Patents
It is a kind of to be used for the method for restoration of the ecosystem to tooth moss Download PDFInfo
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- CN107135792A CN107135792A CN201710481072.6A CN201710481072A CN107135792A CN 107135792 A CN107135792 A CN 107135792A CN 201710481072 A CN201710481072 A CN 201710481072A CN 107135792 A CN107135792 A CN 107135792A
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- tooth moss
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Abstract
It is used for the method for restoration of the ecosystem to tooth moss the invention discloses a kind of.Comprise the following steps：Collection is north to tooth moss cauline leaf gametophyte and spore, is air-dried 5 days；Polishing is crushed to 50 100 mesh with 1G:20L ratio is inoculated in bog moss and the mixed-matrix of coconut palm chaff；It is 23 DEG C, 80 μm of ol photons m in temperature‑2s‑1Grown 2 weeks in light intensity, the incubator under 16 hour long-day, to increase biomass, during which calculate growth efficiency with Stereo microscope and the micro- border observation of inversion；Testing site first prepares the BCD culture medium coatings of one layer of improvement, then will grow uniform broadcasting after 2 weeks crushed to tooth moss and substrate mixture and on coating, covers blue Nylon net, daily 3 moisturizings of spraying, greening cover degree is complete after 10 minutes every time, one month.The present invention utilizes fast growth, and extremely strong dehydration tolerance is green material application to tooth moss, applies to restoration of the ecosystem to tooth moss for the later stage and provides technological guidance.
Description
Art
The invention belongs to biological technical field, and in particular to a kind of to be used for the method for restoration of the ecosystem to tooth moss, but not limit to
In sharp leaf to tooth moss.
Background technology
Liver moss is the green higher plant that first is logged in, accommodation and wide, and the effect in the ecosystem is to closing weight
Will, such as moss crust occupies more than 40% (Wei Jiangchun, 2005) of vegetative coverage in desert.There is case to show liver moss in arid
It is huge for water and soil conservation and air nitrogen cycle contribution with semiarid zone.The special flag artificial infection shape of the drawing of Ku Buqi desert edge
Into moss crust so that also there occurs to table soil property obvious change, wherein grains of sand content is gradually decreased, powder, sticky content
Increase (Wu et al., 2014；Young&Reed,2017).In the vegetation-covered area for having moss crust substantially than no liver moss knot
The local soil and water conservation effect of skin is good, weak the problem of vegetation degeneration.Liver moss can grow as pionner in extreme environment,
Better than the green plantss such as other height (Baquiran et al., 2016；Deane-Coe&Stanton,2017).Process of Urbanization Construction
Quickening and mankind's activity cause increasingly severe environmental destruction, massif is damaged, and slope of highway is exposed and " five adopt
The destruction of area " large area.Repair with restored ecosystem to China environmental protection, ecological construction, agricultural production and social economy
Sustainable development is respectively provided with important realistic meaning.
Sharp leaf is that Yunnan hillside, rock face are extensive to tooth moss (Didymodon constrictus (Mitt.) K.Saito)
A kind of liver moss of distribution, habitat is extremely wide, had both adapted to the moist darkness of sylvan life, sun-drenched place is adapted to again.With resistance to poor
Lean, high temperature resistant, low temperature, growth be fast and a variety of advantages, liver moss kind gable moss or the red moss of tooth rib with extreme drought resisting such as dehydration tolerance
Breeding cycle etc. nearly 1 year, which is compared, has application advantage.It is rapid as pioneer growth, can be the in disturbed habitat
One time formed group and accelerates microhabitat regeneration rate.The present invention, using it as material, is to apply to restoration of the ecosystem to tooth moss in the later stage
Technological guidance is provided.
The content of the invention
The present invention is intended to provide a kind of be used for the method for restoration of the ecosystem, including expanding propagation to tooth moss, field planting to tooth moss
In experimental field, maintenance and monitoring method.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
It is a kind of to be used for the method for restoration of the ecosystem to tooth moss, this method include to the expanding propagation of tooth moss, be colonized in experimental field,
Maintenance and monitoring step.
A kind of as mentioned to be used for the method for restoration of the ecosystem to tooth moss, this method comprises the following steps：Collection is to tooth moss stem
Leaf gametophyte and spore are north, are air-dried 5 days；Polishing is crushed to 50-100 mesh with 1G:20L ratio is inoculated in bog moss and coconut palm
The mixed-matrix of chaff；It is 23 DEG C, 80 μm of ol photons m in temperature-2s-1It is raw in light intensity, the incubator under 16 hour long-day
It is long 2 weeks, during which calculate growth efficiency with Stereo microscope and the micro- border observation of inversion；Testing site first prepares one layer of improvement BCD training
Base coating is supported, then will grow after 2 weeks crushed to tooth moss and substrate mixture that uniform broadcasting is on coating, covering blue Nylon
Net, daily 3 moisturizings of spraying are opened after 10 minutes every time, one month and grown under nylon wire natural conditions.
It is a kind of as mentioned to be used for the method for restoration of the ecosystem to tooth moss, wherein described polishing, which is crushed, is crushed using Chinese medicine
Machine, parameter is set to 50-100 mesh.
It is a kind of as mentioned to be used for the method for restoration of the ecosystem to tooth moss, wherein described coconut palm chaff is by washing, except desalt from
Son, then with bog moss 1:5 mixing.
A kind of as mentioned to be used for the method for restoration of the ecosystem to tooth moss, culture medium, which is set, to be made using BCD basal mediums
For background culture medium, improvement BCD culture medium prescriptions therein are：MgSO4.7H21 μM of O, KH2PO418.4 μM, KNO310 μM,
FeSO4.7H2O 45μM；CuSO4.5H20.22 μM of O, H3BO310 μM, CoCl2.6H20.23 μM of O, Na2MoO4.2H2O 0.1μ
M, ZnSO4.7H20.19 μM of O, MnCl2.4H20.17 μM of 2 μM of O, KI, 5mM CaCl2, agar 1%, according to the composition one by one
It is added in boiling water and dissolves, cool to 45 DEG C is experimental field being made coating.
A kind of as mentioned to be used for the method for restoration of the ecosystem to tooth moss, wherein liver moss gametophyte growth conditions observation uses body
Formula microscopic, and photograph to record daily, contrast branch and growth conditions, with student ' s t-test statistical analyses.
A kind of described method for being used for restoration of the ecosystem to tooth moss is used to cause the vegetation of breakage because building, repairing the roads, open a mine
Restoration of the ecosystem.
A kind of described method for being used for restoration of the ecosystem to tooth moss is used for soil, Shi Zhi, the vegetation of the various gradients of cement
Repair.
It is used for the method for restoration of the ecosystem to tooth moss, wherein the big hypnum moss during described culture 2 weeks simultaneously with equally handling
Compareed with the blue or green moss of wrinkle leaf, it is found that the recovery to tooth moss is most fast, growth is most vigorous.
Brief description of the drawings
Fig. 1 grows 2 weeks posterior division number situations and counted for the present invention to tooth moss, the blue or green moss of wrinkle leaf, big hypnum moss.
Embodiment
Below in conjunction with the accompanying drawings, further illustrated with embodiments of the invention the present invention essentiality content, but not with
This limits the present invention.
Embodiment 1：
1. material and method：
1.1 research material：
The present invention is three kinds of liver mosses using material, and sharp leaf is to tooth moss (Didymodon constrictus (Mitt.)
K.Saito), the blue or green moss (Brachythecium kuroishicum Besch.) of wrinkle leaf and big hypnum moss (Hypnum
Plumaeforme Wilson), collecting location is Kunming Inst. of Botany, Chinese Academy of Sciences greenhouse, and particular location is
Alt.1938m, 25 ° of 9 〞 of ˊ 19 of north latitude, 101 ° of 44 〞 of ˊ 35 of east longitude.
1.2 research method：
1.2.1 experimental design：The sharp leaf of collection is north to tooth moss cauline leaf gametophyte and spore, is air-dried 5 days；Polishing is crushed to
50-100 mesh is with 1G:20L ratio is inoculated in bog moss and the mixed-matrix of coconut palm chaff；It is 23 DEG C, 80 μm of ol in temperature
photons m-2s-1Grown in light intensity, the incubator under 16 hour long-day 2 weeks, it is during which micro- with Stereo microscope and inversion
Border observation calculates growth efficiency；Testing site first prepares the BCD culture medium coatings of one layer of improvement, then will grow the sharp leaf pair of 2 weeks
Uniform broadcasting covers blue Nylon net, daily 3 moisturizings of spraying on coating after tooth moss and substrate mixture are crushed, and every time 10
Minute, open and grown under nylon wire natural conditions after one month.
1.2.2 gametophyte inoculation technique：The mechanical grinding of vegetable material is carried out using medicinal herb grinder, 1G vegetable materials are mixed
Together in 20L bog mosses and the mixed-matrix of coconut palm chaff, 1-5MM thickness is paved into, 23 DEG C, 80 μm of ol photons m are placed in-2s-1Light
By force, the 16h long-day cultivates.
1.2.3 culture medium is configured：MgSO4.7H21 μM of O, KH2PO418.4 μM, KNO310 μM, FeSO4.7H2O 45μM；
CuSO4.5H20.22 μM of O, H3BO310 μM, CoCl2.6H20.23 μM of O, Na2MoO4.2H20.1 μM of O, ZnSO4.7H2O 0.19
μM, MnCl2.4H20.17 μM of 2 μM of O, KI, 5mM CaCl2, agar 1%, according to the composition be added to one by one in boiling water dissolve,
Cool to 45 DEG C are experimental field being made coating.Change formula and with the addition of calcium ion, the growth of cell membrane when being conducive to cell amount reproduction.
1.2.4 growth conditions are observed：Taken a picture using Stereo microscope, 3 kinds of liver moss each time points are selected at random no less than 5
Individual microphoto, counts branch amount, with student ' s t-test statistical analyses.The Stereo microscope instrument type that the present invention is used
Number：Olympus SZX16.
2. result
2.1 are grown by 2 weeks posterior division number situations and is counted for tooth moss, the blue or green moss of wrinkle leaf, big hypnum moss：
The Somatic Embryogenesis of liver moss is very competent, is possible to carry out amount reproduction after the mechanical crushing of plant material.
But sporangium is only produced when temperature alternately changes, but abortion rate is higher, therefore, is highly advantageous using gametophyte breeding
Approach.In order to compare the power of regeneration of liver moss not of the same race, 3 kinds of comprehensive anti-adversity abilities of selection are stronger, adapt to extreme habitat liver moss
Experiment, observes after being inoculated with and spray water after same crushing, recovers green immediately to tooth moss, illustrate under this condition of culture to tooth
The recovery capability of moss is most strong.Observed after 5 days, 3 kinds of liver mosses all sprout shoot, the sprouting of the blue or green moss of wrinkle leaf is most slow.Grow 2 Zhou Houtong
Branch amount is counted, is found most to the quantity of tooth moss, it was demonstrated that possess most vigorous meristematic capacity to tooth moss.Abscissa display growth day
Number, ordinate shows branch amount.Asterisk shows t-test inspections when the numerical value is compared with the unified blue or green moss branch amount of growth period wrinkle leaf
Survey in p<Significant difference in 0.01 level；Data are expressed as average value ± standard error, n≤5 (Fig. 1).
2.2 experimental field in situation about being inoculated with before and after growing January to tooth moss
Fertility to tooth moss is extremely strong, after field planting spraying keeps certain humidity growth 1 month on BCD coatings, liver moss
Surface is successfully covered in, there is obvious afforestation effect.
Claims (8)
1. a kind of be used for the method for restoration of the ecosystem to tooth moss, it is characterised in that this method includes the expanding propagation to tooth moss, field planting
In experimental field, maintenance and monitoring step.
2. a kind of method to tooth moss for restoration of the ecosystem as claimed in claim 1, it is characterised in that this method includes following
Step：Collection is north to tooth moss cauline leaf gametophyte and spore, is air-dried 5 days；Polishing is crushed to 50-100 mesh with 1G:20L ratio
Example is inoculated in bog moss and the mixed-matrix of coconut palm chaff；It is 23 DEG C, 80 μm of ol photons m in temperature-2s-1Light intensity, 16 small durations
Grown in incubator under sunshine 2 weeks, during which calculate growth efficiency with Stereo microscope and the micro- border observation of inversion；Testing site is first
One layer of improvement BCD culture medium coating is prepared, uniform broadcasting then will be grown after 2 weeks crushed to tooth moss and substrate mixture in painting
On layer, blue Nylon net is covered, daily 3 moisturizings of spraying, opened after 10 minutes every time, one month raw under nylon wire natural conditions
It is long.
3. a kind of method to tooth moss for restoration of the ecosystem as claimed in claim 2, it is characterised in that wherein described polishing
Crushing is to use medicinal herb grinder, and parameter is set to 50-100 mesh.
4. a kind of method to tooth moss for restoration of the ecosystem as claimed in claim 2, it is characterised in that wherein described coconut palm chaff
By washing, salt ion is removed, then with bog moss 1:5 mixing.
5. a kind of method to tooth moss for restoration of the ecosystem as claimed in claim 2, it is characterised in that it is profit that culture medium, which is set,
With BCD basal mediums as background culture medium, improvement BCD culture medium prescriptions therein are：MgSO4.7H21 μM of O, KH2PO4
18.4 μM, KNO310 μM, FeSO4.7H2O 45μM；CuSO4.5H20.22 μM of O, H3BO310 μM, CoCl2.6H20.23 μM of O,
Na2MoO4.2H20.1 μM of O, ZnSO4.7H20.19 μM of O, MnCl2.4H20.17 μM of 2 μM of O, KI, 5mM CaCl2, agar 1%,
It is added in boiling water and dissolves one by one according to the composition, cool to 45 DEG C is experimental field being made coating.
6. a kind of it is used for the method for restoration of the ecosystem to tooth moss as claimed in claim 2, it is characterised in that wherein liver moss gametophyte
Growth conditions observation is photographed to record daily using Stereo microscope observation, contrast branch and growth conditions, with student '
St-test statistical analyses.
7. a kind of method for being used for restoration of the ecosystem to tooth moss described in claim 1 or 2 is used for because building, repairing the roads, opening a mine causes
The restoration of the ecosystem of damaged vegetation.
8. described in claim 1 or 2 it is a kind of to tooth moss be used for restoration of the ecosystem method be used for soil, Shi Zhi, cement it is various
The vegetation repair of the gradient.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112568105A (en) * | 2020-12-04 | 2021-03-30 | 贵州省山地资源研究所 | Ecological greening method for bare rock mass |
| CN113243257A (en) * | 2021-06-09 | 2021-08-13 | 四川大学 | Ecological slag greening method and application |
| CN115299329A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Method for culturing odontopathy and application thereof |
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| JP2004057122A (en) * | 2002-07-31 | 2004-02-26 | Seia:Kk | Greening apparatus using moss and method for constructing the same apparatus |
| CN1613289A (en) * | 2004-12-13 | 2005-05-11 | 陈思 | Method for controlling rock desert by bryophyte |
| CN104380959A (en) * | 2014-10-20 | 2015-03-04 | 西北农林科技大学 | Rapid cultivation method for loess plateau moss crusts |
| CN106359092A (en) * | 2016-08-30 | 2017-02-01 | 中国科学院昆明植物研究所 | Rapid propagation method for physcomitrella patens protonemata |
| CN106472281A (en) * | 2016-10-14 | 2017-03-08 | 中国科学院水生生物研究所 | A kind of liver moss is combined the breeding method of skinning with desert algae |
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2017
- 2017-06-22 CN CN201710481072.6A patent/CN107135792A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004057122A (en) * | 2002-07-31 | 2004-02-26 | Seia:Kk | Greening apparatus using moss and method for constructing the same apparatus |
| CN1613289A (en) * | 2004-12-13 | 2005-05-11 | 陈思 | Method for controlling rock desert by bryophyte |
| CN104380959A (en) * | 2014-10-20 | 2015-03-04 | 西北农林科技大学 | Rapid cultivation method for loess plateau moss crusts |
| CN106359092A (en) * | 2016-08-30 | 2017-02-01 | 中国科学院昆明植物研究所 | Rapid propagation method for physcomitrella patens protonemata |
| CN106472281A (en) * | 2016-10-14 | 2017-03-08 | 中国科学院水生生物研究所 | A kind of liver moss is combined the breeding method of skinning with desert algae |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112568105A (en) * | 2020-12-04 | 2021-03-30 | 贵州省山地资源研究所 | Ecological greening method for bare rock mass |
| CN113243257A (en) * | 2021-06-09 | 2021-08-13 | 四川大学 | Ecological slag greening method and application |
| CN115299329A (en) * | 2022-08-04 | 2022-11-08 | 内蒙古农业大学 | Method for culturing odontopathy and application thereof |
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Application publication date: 20170908 |