CN107098947A - A kind of technique that Asiaticoside is extracted from centella - Google Patents
A kind of technique that Asiaticoside is extracted from centella Download PDFInfo
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- CN107098947A CN107098947A CN201710294526.9A CN201710294526A CN107098947A CN 107098947 A CN107098947 A CN 107098947A CN 201710294526 A CN201710294526 A CN 201710294526A CN 107098947 A CN107098947 A CN 107098947A
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- asiaticoside
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
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Abstract
The invention discloses a kind of technique that Asiaticoside is extracted from centella, belong to Asiaticoside preparing technical field, the Asiaticoside is by crushing, once decolouring, crystallization, secondary decolourization, recrystallization, extract and separate purification, condensing crystallizing, drying and other steps are made.The purity of Asiaticoside is above the purity of Asiaticoside made from prior art, and can be used as qualitative, quantitative reference substance more than 98.02% made from the method for the present invention.
Description
【Technical field】
A kind of Asiaticoside preparing technical field of the present invention, and in particular to technique that Asiaticoside is extracted from centella.
【Background technology】
Centella is the herb or whole herb with root of dicotyledon samphire centella, with clearing heat and promoting diuresis, detumescence
The effect of removing toxic substances；For treating jaundice with damp-heat pathogen, dysentery rubella, larynx are swollen, pyogenic infections and traumatic injury etc..Asiaticoside is that centella carries
Take one of active ingredient in thing.Asiaticoside has multiple pharmacological effect：(1) beauty：Wheat Protein, dispels old dead angle
Lipid layer, promotes the metabolism of skin, elastic and smooth, the extra-nutrition element of increase skin.(2) health care：Endocrine is adjusted, is changed
Kind function of intestinal canal, anti-aging improves memory, improves anaemia, promotes sleep, improves functions of intestines and stomach.(3) lose weight：Courage is reduced to consolidate
Alcohol, removes superabundant fats and reduction is overweight, adjusts the neurotransmitter of brain, supplements the nutrition of cerebral nerve maincenter.(4) wound is promoted
Mouth healing and healing skin injury：Skin ulcer can be treated, such as obstinate sexual trauma, cutaneous tuberculosis, leprosy.To mouse, cavy,
Rabbit muscle is injected or can promote skin growth when being subcutaneously implanted, local leukocytosis, connective tissue rete vasculosum hyperplasia, mucus point
Secrete Acceleration of growth of increase, hair and tail etc..(5) immunologic function is adjusted：Asiaticoside can suppress the formation of autoantibody, and reduction is exempted from
Epidemic disease globulin, improves cellular immune function.(6) antibacterial：To golden yellow staphylococcus, Hemolytic Type streptococcus, various shigella dysenteriaes, wound
Cold bacillus has inhibitory action.(7) antiulcer action：Rat pylorus ligation ulcer and stress ulcer can be suppressed.(8) to maincenter
The effect of nerve：Central nervous system is stimulated, so as to improve memory and antifatigue.(9) calm, stable effect：Available for treating
Insomnia.According to another document report, the ulcer of lower limb and wound that can not heal for a long time that Asiaticoside is caused to venous insufficiency
The surface of a wound caused by case, operation or adhesion of tendon caused by wound, the factor such as burn recover after keloid and chorionitis
There is certain curative effect.Asiaticoside can also slightly suppress the effect of acetylcholine, and for the treatment of the proliferation of mammary gland.
The health care of Asiaticoside, medicinal efficacy are so more, but up to the present, it is few on being extracted from centella
The relevant report of high-purity Asiaticoside.CN1491656A discloses the technique step that Gotu Kola P.E is extracted from centella
Suddenly, but the main component of the extract is Asiaticoside and madecassoside, the two content in extract is 10%-
95%, Asiaticoside and madecassoside are not separated.CN101210040A discloses a kind of asiaticoside chemical control
The method for separating and preparing of product, can obtain the asiaticoside chemical reference substance that purity is more than 98%, but this method is with weight content
30%-97% Asiaticoside extract is that raw material carries out separating-purifying, it is necessary to certain content is first extracted from centella
Asiaticoside extract.CN101323637A discloses a kind of Asiaticoside and preparation method thereof, and obtained Asiaticoside is pure
Degree is more than 95%, and gained sample can not be entirely as qualitatively reference substance.
【The content of the invention】
A kind of technique that Asiaticoside is extracted from centella that the present invention is provided, to solve product made from native prior art
The careless glucoside purity of snow is more than 95%, the problem of gained sample can not be entirely as qualitatively reference substance.
To solve above technical problem, the present invention uses following technical matters：
A kind of technique that Asiaticoside is extracted from centella, comprises the following steps：
S1：Centella is crushed and sifted out, asiatic pennywort Herd is made；
S2：Ethanol is added into asiatic pennywort Herd made from step S1, refluxing extraction 2-3 times under water bath condition, every time
A length of 1.2-1.6h during extraction, merges extract solution after extracting completely, adjusts the pH value of extract solution to 4-4.6, temperature control is 56-
62 DEG C, by decolorization and impurity removal by active carbon, a destainer is made after filtering；
S3：A destainer made from step S2 is concentrated and dried, extraction cream is made, extraction 5-5.4 times of body of cream is used in
42-50 DEG C long-pending of water is dissolved, and is carried out extraction 2-3 times using anhydrous ethyl acetate solvent, is continued to use anhydrous ethyl acetate
It is 1 to carry out solid-to-liquid ratio in 2-3 crystallization treatment, crystallization process:4-5.2 (W/V), is made crystal crude product；
S4：By ethanol dissolving of the coarse-grain made from step S3 through 70%-80%, the pH value of solution is adjusted to 4-4.6, temperature
Control as 56-62 DEG C, by decolorization and impurity removal by active carbon, secondary decolourization liquid is made after filtering；
S5：After secondary decolourization liquid vacuum-concentrcted made from step S4, crystallized in the case where temperature is 6-10 DEG C, weight is made
Crystal crude product；
S6：Crude product will be recrystallized made from step S5 by extraction, Asiaticoside liquid is made；
S7：Asiaticoside liquid made from step S6 is carried out to crystallize and dry after vacuum-concentrcted, centella is made
Glucoside.
Preferably, the mesh number of sieve described in step S1 is 40-60.
Preferably, the weight ratio of asiatic pennywort Herd and ethanol described in step S2 is 1:6-8.
Preferably, the volume fraction of the ethanol is 70%-80%.
Preferably, it is 0.5-1h by the time of decolorization and impurity removal by active carbon described in step S2.
Preferably, it is 0.4-0.7h by the time of decolorization and impurity removal by active carbon described in step S4.
Preferably, extraction described in step S6 is supercritical CO2Extraction.
Preferably, the supercritical CO2The condition of extraction：Extraction kettle pressure is 12-15MPa, and extraction temperature is 40-45
DEG C, extraction time is 28-35min.
Preferably, crystallization described in step S6 is carried out in the case where temperature is 3-8 DEG C.
Preferably, crystal described in step S6 is dried under vacuum to moisture content≤1% in the case where temperature is 40-45 DEG C.
The invention has the advantages that：
The purity of Asiaticoside made from the method for the present invention is above product made from prior art more than 98.02%
The purity of the careless glucoside of snow, and qualitative, quantitative reference substance can be used as.
【Embodiment】
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to the present invention's
Protection domain, but do not limit the scope of the invention.
In embodiment, the technique that Asiaticoside is extracted from centella comprises the following steps：
S1：Centella is crushed and 40-60 mesh sieve is crossed, asiatic pennywort Herd is made；
S2：6-8 times of 70%-80% of its weight ethanol is added into asiatic pennywort Herd made from step S1, in water-bath bar
Refluxing extraction 2-3 times under part, a length of 1.2-1.6h when extracting every time merges extract solution after extracting completely, adjusts the pH of extract solution
Value is to 4-4.6, and temperature control is 56-62 DEG C, by decolorization and impurity removal by active carbon 0.5-1h, and a destainer is made after filtering；
S3：A destainer made from step S2 is concentrated and dried, extraction cream is made, extraction 5-5.4 times of body of cream is used in
42-50 DEG C long-pending of water is dissolved, and is carried out extraction 2-3 times using anhydrous ethyl acetate solvent, is continued to use anhydrous ethyl acetate
It is 1 to carry out solid-to-liquid ratio in 2-3 crystallization treatment, crystallization process:4-5.2 (W/V), is made crystal crude product；
S4：By ethanol dissolving of the coarse-grain made from step S3 through 70%-80%, the pH value of solution is adjusted to 4-4.6, temperature
Control as 56-62 DEG C, by decolorization and impurity removal by active carbon 0.4-0.7h, secondary decolourization liquid is made after filtering；
S5：After secondary decolourization liquid vacuum-concentrcted made from step S4, crystallized in the case where temperature is 6-10 DEG C, weight is made
Crystal crude product；
S6：Pass through supercritical CO by crude product is recrystallized made from step S52Extraction, the condition of extraction：Extraction kettle pressure is
12-15MPa, extraction temperature is 40-45 DEG C, and extraction time is 28-35min, and Asiaticoside liquid is made；
S7：Asiaticoside liquid made from step S6 is carried out after vacuum-concentrcted, tied in the case where temperature is 3-8 DEG C
Crystalline substance, the crystal is dried under vacuum to moisture content≤1% in the case where temperature is 40-45 DEG C, and Asiaticoside is made.
Embodiment 1
A kind of technique that Asiaticoside is extracted from centella, comprises the following steps：
S1：Centella is crushed and 50 mesh sieve are crossed, asiatic pennywort Herd is made；
S2：The ethanol of 7 times 76% of its weight is added into asiatic pennywort Herd made from step S1, is flowed back under water bath condition
Extract 2 times, a length of 1.5h when extracting every time, merge extract solution after extracting completely, adjust the pH value of extract solution to 4.3, temperature control
60 DEG C are made as, by decolorization and impurity removal by active carbon 0.7h, a destainer is made after filtering；
S3：A destainer made from step S2 is concentrated and dried, extraction cream is made, extraction 5.3 times of volumes of cream are used in
46 DEG C of water dissolved, using anhydrous ethyl acetate solvent carry out extraction 2 times, continuation is carried out 2 times with anhydrous ethyl acetate
Solid-to-liquid ratio is 1 in crystallization treatment, crystallization process:4.8 (W/V), are made crystal crude product；
S4：Coarse-grain made from step S3 is dissolved through 75% ethanol, the pH value of solution is adjusted to 4.3, temperature control is
60 DEG C, by decolorization and impurity removal by active carbon 0.6h, secondary decolourization liquid is made after filtering；
S5：After secondary decolourization liquid vacuum-concentrcted made from step S4, crystallized in the case where temperature is 8 DEG C, be made and tie again
Brilliant crude product；
S6：Pass through supercritical CO by crude product is recrystallized made from step S52Extraction, the condition of extraction：Extraction kettle pressure is
14MPa, extraction temperature is 43 DEG C, and extraction time is 32min, and Asiaticoside liquid is made；
S7：Asiaticoside liquid made from step S6 is carried out after vacuum-concentrcted, tied in the case where temperature is 6 DEG C
Crystalline substance, the crystal is that to be dried under vacuum to moisture content at 43 DEG C be 1% in temperature, and Asiaticoside is made.
Embodiment 2
A kind of technique that Asiaticoside is extracted from centella, comprises the following steps：
S1：Centella is crushed and 40 mesh sieve are crossed, asiatic pennywort Herd is made；
S2：The ethanol of 6 times 70% of its weight is added into asiatic pennywort Herd made from step S1, is flowed back under water bath condition
Extract 2 times, a length of 1.2h when extracting every time, merge extract solution after extracting completely, adjust the pH value of extract solution to 4, temperature control
For 56 DEG C, by decolorization and impurity removal by active carbon 1h, a destainer is made after filtering；
S3：A destainer made from step S2 is concentrated and dried, extraction cream is made, extraction 5 times of volumes of cream are used in
42 DEG C of water is dissolved, and extraction 2 times is carried out using anhydrous ethyl acetate solvent, and continuation carries out 2 knots with anhydrous ethyl acetate
Solid-to-liquid ratio is 1 in crystalline substance processing, crystallization process:4 (W/V), are made crystal crude product；
S4：Coarse-grain made from step S3 is dissolved through 70% ethanol, the pH value of solution is adjusted to 4, temperature control is 56
DEG C, by decolorization and impurity removal by active carbon 0.7h, secondary decolourization liquid is made after filtering；
S5：After secondary decolourization liquid vacuum-concentrcted made from step S4, crystallized in the case where temperature is 6 DEG C, be made and tie again
Brilliant crude product；
S6：Pass through supercritical CO by crude product is recrystallized made from step S52Extraction, the condition of extraction：Extraction kettle pressure is
12MPa, extraction temperature is 40 DEG C, and extraction time is 35min, and Asiaticoside liquid is made；
S7：Asiaticoside liquid made from step S6 is carried out after vacuum-concentrcted, tied in the case where temperature is 3 DEG C
Crystalline substance, the crystal is that to be dried under vacuum to moisture content at 40 DEG C be 0.8% in temperature, and Asiaticoside is made.
Embodiment 3
A kind of technique that Asiaticoside is extracted from centella, comprises the following steps：
S1：Centella is crushed and 40 mesh sieve are crossed, asiatic pennywort Herd is made；
S2：The ethanol of 8 times 80% of its weight is added into asiatic pennywort Herd made from step S1, is flowed back under water bath condition
Extract 3 times, a length of 1.5h when extracting every time, merge extract solution after extracting completely, adjust the pH value of extract solution to 4.6, temperature control
62 DEG C are made as, by decolorization and impurity removal by active carbon 0.5h, a destainer is made after filtering；
S3：A destainer made from step S2 is concentrated and dried, extraction cream is made, extraction 5.4 times of volumes of cream are used in
50 DEG C of water dissolved, using anhydrous ethyl acetate solvent carry out extraction 3 times, continuation is carried out 3 times with anhydrous ethyl acetate
Solid-to-liquid ratio is 1 in crystallization treatment, crystallization process:5.2 (W/V), are made crystal crude product；
S4：Coarse-grain made from step S3 is dissolved through 80% ethanol, the pH value of solution is adjusted to 4.6, temperature control is
62 DEG C, by decolorization and impurity removal by active carbon 0.4h, secondary decolourization liquid is made after filtering；
S5：After secondary decolourization liquid vacuum-concentrcted made from step S4, crystallized in the case where temperature is 10 DEG C, be made and tie again
Brilliant crude product；
S6：Pass through supercritical CO by crude product is recrystallized made from step S52Extraction, the condition of extraction：Extraction kettle pressure is
15MPa, extraction temperature is 45 DEG C, and extraction time is 28min, and Asiaticoside liquid is made；
S7：Asiaticoside liquid made from step S6 is carried out after vacuum-concentrcted, tied in the case where temperature is 8 DEG C
Crystalline substance, the crystal is that to be dried under vacuum to moisture content at 45 DEG C be 0.9% in temperature, and Asiaticoside is made.
Comparative example 1, using a kind of " Asiaticoside and preparation method thereof (patent No.：ZL200810303151.9 implementation) "
Example 1-3 method prepares Asiaticoside.
Purity to Asiaticoside made from embodiment 1-3 and comparative example 1 is detected, as a result as shown in the table.
| Experimental project | Purity (%) |
| Embodiment 1 | 98.12 |
| Embodiment 2 | 98.02 |
| Embodiment 3 | 98.61 |
| Comparative example 1 | 95.62-96.03 |
From with upper table, the purity of Asiaticoside made from method of the invention is above existing more than 98.02%
The purity of Asiaticoside made from technology, and qualitative, quantitative reference substance can be used as.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to assert
The specific implementation of the present invention is confined to these explanations, for person of an ordinary skill in the technical field, is not departing from
On the premise of present inventive concept, some simple deduction or replace can also be made, the present invention should be all considered as belonging to by being submitted
Claims determine scope of patent protection.
Claims (10)
1. a kind of technique that Asiaticoside is extracted from centella, it is characterised in that comprise the following steps：
S1：Centella is crushed and sifted out, asiatic pennywort Herd is made；
S2：Ethanol is added into asiatic pennywort Herd made from step S1, refluxing extraction 2-3 times under water bath condition is extracted every time
Shi Changwei 1.2-1.6h, merge extract solution after extracting completely, adjust the pH value of extract solution to 4-4.6, temperature control is 56-62
DEG C, by decolorization and impurity removal by active carbon, a destainer is made after filtering；
S3：A destainer made from step S2 is concentrated and dried, extraction cream is made, extraction 5-5.4 times of volume of cream is used in
42-50 DEG C of water is dissolved, and carries out extraction 2-3 times using anhydrous ethyl acetate solvent, continuation is carried out with anhydrous ethyl acetate
Solid-to-liquid ratio is 1 in 2-3 crystallization treatment, crystallization process:4-5.2 (W/V), is made crystal crude product；
S4：By ethanol dissolving of the coarse-grain made from step S3 through 70%-80%, the pH value of solution is adjusted to 4-4.6, temperature control
For 56-62 DEG C, by decolorization and impurity removal by active carbon, secondary decolourization liquid is made after filtering；
S5：After secondary decolourization liquid vacuum-concentrcted made from step S4, crystallized in the case where temperature is 6-10 DEG C, recrystallization is made
Crude product；
S6：Crude product will be recrystallized made from step S5 by extraction, Asiaticoside liquid is made；
S7：Asiaticoside liquid made from step S6 is carried out to crystallize and dry after vacuum-concentrcted, Asiaticoside is made.
2. the technique according to claim 1 that Asiaticoside is extracted from centella, it is characterised in that：Described in step S1
The mesh number of sieve is 40-60.
3. the technique according to claim 1 that Asiaticoside is extracted from centella, it is characterised in that：Described in step S2
The weight ratio of asiatic pennywort Herd and ethanol is 1:6-8.
4. the technique according to claim 3 that Asiaticoside is extracted from centella, it is characterised in that：The body of the ethanol
Fraction is 70%-80%.
5. the technique according to claim 1 that Asiaticoside is extracted from centella, it is characterised in that：Described in step S2
Time by decolorization and impurity removal by active carbon is 0.5-1h.
6. the technique according to claim 1 that Asiaticoside is extracted from centella, it is characterised in that：Described in step S4
Time by decolorization and impurity removal by active carbon is 0.4-0.7h.
7. the technique according to claim 1 that Asiaticoside is extracted from centella, it is characterised in that：Described in step S6
Extract as supercritical CO2Extraction.
8. the technique according to claim 7 that Asiaticoside is extracted from centella, it is characterised in that：The supercritical CO2
The condition of extraction：Extraction kettle pressure is 12-15MPa, and extraction temperature is 40-45 DEG C, and extraction time is 28-35min.
9. the technique according to claim 1 that Asiaticoside is extracted from centella, it is characterised in that：Described in step S6
Crystallization is carried out in the case where temperature is 3-8 DEG C.
10. the technique according to claim 9 that Asiaticoside is extracted from centella, it is characterised in that：Institute in step S6
State crystal and be dried under vacuum to moisture content≤1% in the case where temperature is 40-45 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112957764A (en) * | 2021-01-29 | 2021-06-15 | 三益创价生物科技(深圳)有限公司 | Method for extracting asiaticoside-rich composition from centella asiatica |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210040A (en) * | 2006-12-27 | 2008-07-02 | 中国科学院大连化学物理研究所 | Method for separating and preparing asiaticoside chemical reference substance |
| CN101323637A (en) * | 2008-07-29 | 2008-12-17 | 卢照凯 | Asiaticoside and preparation thereof |
| CN102532244A (en) * | 2011-12-31 | 2012-07-04 | 广西大学 | Method for preparing high-purity asiaticosid |
| CN102603854A (en) * | 2011-01-25 | 2012-07-25 | 苏州宝泽堂医药科技有限公司 | Method for extracting asiaticoside from asiatic pennywort herb |
| CN104892719A (en) * | 2014-12-04 | 2015-09-09 | 陕西君碧莎制药有限公司 | Novel efficient preparation method for asiaticoside |
-
2017
- 2017-04-28 CN CN201710294526.9A patent/CN107098947A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101210040A (en) * | 2006-12-27 | 2008-07-02 | 中国科学院大连化学物理研究所 | Method for separating and preparing asiaticoside chemical reference substance |
| CN101323637A (en) * | 2008-07-29 | 2008-12-17 | 卢照凯 | Asiaticoside and preparation thereof |
| CN102603854A (en) * | 2011-01-25 | 2012-07-25 | 苏州宝泽堂医药科技有限公司 | Method for extracting asiaticoside from asiatic pennywort herb |
| CN102532244A (en) * | 2011-12-31 | 2012-07-04 | 广西大学 | Method for preparing high-purity asiaticosid |
| CN104892719A (en) * | 2014-12-04 | 2015-09-09 | 陕西君碧莎制药有限公司 | Novel efficient preparation method for asiaticoside |
Non-Patent Citations (1)
| Title |
|---|
| 侯文砚: "积雪草苷的提取与分离", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112957764A (en) * | 2021-01-29 | 2021-06-15 | 三益创价生物科技(深圳)有限公司 | Method for extracting asiaticoside-rich composition from centella asiatica |
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Application publication date: 20170829 |