CN1070945A - The method of induced Japanese oyster producing triploid - Google Patents
The method of induced Japanese oyster producing triploid Download PDFInfo
- Publication number
- CN1070945A CN1070945A CN92106666A CN92106666A CN1070945A CN 1070945 A CN1070945 A CN 1070945A CN 92106666 A CN92106666 A CN 92106666A CN 92106666 A CN92106666 A CN 92106666A CN 1070945 A CN1070945 A CN 1070945A
- Authority
- CN
- China
- Prior art keywords
- oyster
- triploid
- tetraploid
- cytochalasin
- zygote
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the heritable variation engineering, be a kind of method of induced Japanese oyster producing triploid specifically, it is characterized in that collecting the smart ovum of Japan real oyster and make its fertilization, in 1min～3min, apply cytochalasin B, handle and zygote (being tetraploid this moment) to be taken out behind 20min～28min and in washing rearmounted people's seawater, hatch, hatching back gained larva is sophisticated tetraploid through growing, tetraploid with sex gland mature is a male parent, the diploid Japan real oyster is that female parent is hybridized, thereby obtains triploid zygote.The present invention uses the quantity of cytochalasin B few, has reduced production cost, has alleviated the influence to environment and staff's health, is suitable for big water body and cultivates the triploid Japan real oyster.
Description
The invention belongs to the heritable variation engineering, specifically a kind of method of induced Japanese oyster producing triploid.
Because triploid sterility being used for the glycogen that shellfish sexual gland is grown originally, is used on the muscle growth, in recent years, the triploid technology has been widely used in aquaculture field, and it uses two kinds of methods usually: the one, and the physical agent revulsion is as hydrostaticpressure, cold stimulation, heat-shocked; The 2nd, the chemokines revulsion is as cytochalasin B, colchicine, caffeine etc." ocean lakes and marhshes circular " 92 year the 2nd phase discloses one piece＜cytochalasin B and induced chlamys farreri to produce triploid research 〉, this article has been introduced a kind of method of handling chlamys farreri acquisition autotriploid with cytochalasin B, its treating processes is: one, selectivity gland maturity chlamys farreri is as parent, open shell and obtain sexual gland, shred its sexual gland, collect smart, ovum respectively; Two, the mixed ovum fertilization that makes of smart, ovum, after fertilization stoped the release of first polar body or second polar body by stimulating zygote, thereby has directly formed triploid with the cytochalasin B processing in 10 minutes; Three, the triploid zygote that is produced through hatch, adhere to, juvenile mollusk, forming stage grow and be ripe triploid chlamys farreri.The triploid of adopting this mode of inducing to obtain is an autotriploid, is the karyomit(e) of sexual cell self, and offspring's growth vigor is not really remarkable; In addition, the triploid induction rate instability is generally between 17%～50%; Moreover, obtain a large amount of triploid seedling, the necessary more pharmaceutical chemicals of usage quantity, on the one hand big water body uses the productive expense costliness, and waste liquid all exists certain influence to staff's health and environment on the other hand.
Purpose of the present invention just is to provide a kind of method of induced Japanese oyster producing triploid, and its production cost is low, to staff's health and environmental influence is little and triploid is an allopolyploid.
To achieve the above object of the invention, the present invention can realize by following measure: the female and male Japan real oyster of selectivity gland maturity, and collect its essence respectively, ovum, ovum is put into through the detoxicating treatment temp at 21 ℃ ± 1 ℃ seawater, under the condition of inflation, add seminal fluid, in 1 minute～3 minutes, apply cytochalasin B and stimulated zygote 20～28 minutes, though the interior karyomit(e) of zygote this moment duplicates, but tenuigenin can not divide, thereby formed autotetraploid, tetraploid zygote is put into the nursery pond hatching behind the repetitive scrubbing in the seawater of cleaning, the resulting larva in hatching back is through adhering to, abnormal, juvenile mollusk, forming stage is reached maturity, tetraploid with sex gland mature is a male parent, the diploid Japan real oyster is that female parent is hybridized, thereby obtain allotriploid zygote, because triploid acquisition is to rely on tetraploid and diploid hybrid, that is to say only needs the tetraploid of comparatively small amt can obtain a lot of triploid of quantity as the parent, thereby reduced the consumption of cytochalasin B, reduced production cost, alleviated influence environment and operator's health.
Poverty of the present invention can reach by following measure, and the concentration after cytochalasin B applies is 0.8ppm～1.5ppm.
Chemical induction drug dosage of the present invention is little, can reduce production costs significantly, less waste liquid can not cause staff's health and environment and seriously influence, tetraploid inductivity height, moreover triploid is to produce behind tetraploid and the diploid hybrid, be allotriploid, can show stronger growth vigor.
Most preferred embodiment: Japan real oyster is a dioecism, outward appearance can not be told sex, gonadal maturation when 20 ℃～22 ℃ of water temperatures, select the individuality of shell length more than 12cm, with cutter breakdown shell, taking-up sexual gland is placed in the seawater and shreds, and leaches foreign material with 120 mesh sieve tulles again, and smart, ovum are collected respectively; The ovum of collecting is put into the small water of handling 21 ℃ seawater through detoxicating, under the condition of inflation, add seminal fluid, in water body, apply the cytochalasin B of 1ppm after 2 minutes, stir evenly, after 25 minutes zygote is shifted out, and put into clean seawater repetitive scrubbing, though the zygote of this moment is duplicated because of the stimulation karyomit(e) of the loose B of cell, but tenuigenin does not divide, thereby formation autotetraploid, tetraploid rate are 62%, resulting tetraploid zygote is put into nursery pond hatch, hatching required condition is: 20 ℃～23 ℃ of water temperatures, dissolved oxygen content more than 5ml/L, 30～40/ml of zygote density, continuous charge, salinity 28-32%, potential of hydrogen 7.8～8.2.Rearing condition larva routinely through adhere to, metamorphosis, juvenile mollusk, forming stage reaches maturity; The tetraploid Cheng Beiwei male parent of selectivity gland maturity then, the diploid Japan real oyster adopts conventional method to hybridize for maternal, finally obtains allotriploid.
The method of induced Japanese oyster producing triploid provided by the present invention, also can realize according to method shown in the following chart, the time that wherein applies cytochalasin B is meant sperm and the ovum time after mixed, and the treatment time is meant the action time that adds behind the cytochalasin B zygote.
Claims (2)
1, a kind of method of induced Japanese oyster producing triploid, at first female, the male diploid Japan real oyster of selectivity gland maturity is as parent, open the parent shell and take out sexual gland, in water, shred its sexual gland, leach foreign material with the bolting silk net, and smart, ovum collected respectively, and ovum is put into sterile-processed water temperature at 21 ℃ ± 1 ℃ seawater
It is characterized in that:
A, after adding sperm, apply cytochalasin B in 1～3 minute,
B, the lasting processing after 20 minutes～28 minutes are shifted out zygote (duplicate because of karyomit(e) this moment, and tenuigenin does not divide, thereby form autotetraploid), in the seawater of cleaning, put into nursery pond behind the repetitive scrubbing to hatch,
C, hatching gained tetraploid larva set after metamorphosis, juvenile mollusk, forming stage reach maturity,
D, be male parent with the tetraploid Japan real oyster of sex gland mature, the diploid Japan real oyster is that female parent is hybridized, thereby obtains triploid zygote.
2,, it is characterized in that it is 0.8ppm～1.5ppm that said cytochalasin B applies concentration according to the method for the described induced Japanese oyster producing triploid of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92106666A CN1070945A (en) | 1992-08-13 | 1992-08-13 | The method of induced Japanese oyster producing triploid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92106666A CN1070945A (en) | 1992-08-13 | 1992-08-13 | The method of induced Japanese oyster producing triploid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1070945A true CN1070945A (en) | 1993-04-14 |
Family
ID=4942317
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN92106666A Pending CN1070945A (en) | 1992-08-13 | 1992-08-13 | The method of induced Japanese oyster producing triploid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1070945A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0752814A4 (en) * | 1994-01-21 | 1997-03-12 | Univ Rutgers | Tetraploid shellfish |
| CN1076579C (en) * | 1997-09-09 | 2001-12-26 | 中国科学院海洋研究所 | Method for breeding young of prawns in three times size of general ones |
| CN102100197A (en) * | 2010-12-20 | 2011-06-22 | 山东海益宝水产股份有限公司 | Method for breading triploid monomer oysters |
| CN101077063B (en) * | 2006-05-26 | 2012-04-25 | 中国科学院海洋研究所 | Method for breeding triploid monomer oyster in scale |
| CN101677524B (en) * | 2007-03-23 | 2012-11-21 | 法国海洋开发研究院 | Production of bivalve tetraploid molluscs from diploid parents |
| CN108124801A (en) * | 2018-03-13 | 2018-06-08 | 中国海洋大学 | A kind of abductive approach of long oyster " sea is No. 2 big " new varieties tetraploid |
| CN114208735A (en) * | 2021-12-22 | 2022-03-22 | 中国科学院南海海洋研究所 | Method for cultivating rapid-growth new strain of hong Kong oyster triploid by backcross breeding technology |
| CN114208734A (en) * | 2021-12-22 | 2022-03-22 | 中国科学院南海海洋研究所 | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies |
-
1992
- 1992-08-13 CN CN92106666A patent/CN1070945A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0752814A4 (en) * | 1994-01-21 | 1997-03-12 | Univ Rutgers | Tetraploid shellfish |
| EP0945503A1 (en) * | 1994-01-21 | 1999-09-29 | Rutgers, The State University Of New Jersey | Tetraploid shellfish |
| CN1076579C (en) * | 1997-09-09 | 2001-12-26 | 中国科学院海洋研究所 | Method for breeding young of prawns in three times size of general ones |
| CN101077063B (en) * | 2006-05-26 | 2012-04-25 | 中国科学院海洋研究所 | Method for breeding triploid monomer oyster in scale |
| CN101677524B (en) * | 2007-03-23 | 2012-11-21 | 法国海洋开发研究院 | Production of bivalve tetraploid molluscs from diploid parents |
| CN102100197A (en) * | 2010-12-20 | 2011-06-22 | 山东海益宝水产股份有限公司 | Method for breading triploid monomer oysters |
| CN108124801A (en) * | 2018-03-13 | 2018-06-08 | 中国海洋大学 | A kind of abductive approach of long oyster " sea is No. 2 big " new varieties tetraploid |
| CN114208735A (en) * | 2021-12-22 | 2022-03-22 | 中国科学院南海海洋研究所 | Method for cultivating rapid-growth new strain of hong Kong oyster triploid by backcross breeding technology |
| CN114208734A (en) * | 2021-12-22 | 2022-03-22 | 中国科学院南海海洋研究所 | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies |
| CN114208735B (en) * | 2021-12-22 | 2023-02-28 | 中国科学院南海海洋研究所 | Method for cultivating rapid-growth new strain of hong Kong oyster triploid by backcross breeding technology |
| CN114208734B (en) * | 2021-12-22 | 2023-02-28 | 中国科学院南海海洋研究所 | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101375677B (en) | Method for culturing mature fish of Erythroculter ilishaeformis | |
| CN102669046B (en) | Method for ecologically culturing Chinese soft shell turtle by using nuisanceless pond | |
| CN106172117B (en) | Little yellow croaker raun and Larimichthys crocea milter cross breeding method | |
| CN100584201C (en) | Mandarin fish comprehensive culture method in mid-latitude region | |
| CN105165666A (en) | Method for ecologically culturing mylopharyngodon piceus by using pollution-free pond | |
| CN105475193B (en) | Family's pond intercropping method of channel catfishes and mandarin fish | |
| CN103404453A (en) | Freshwater shrimp and river crab double main farming method | |
| CN102669047A (en) | Nuisanceless ecological culturing method for Chinese soft shell turtle | |
| CN107182853A (en) | A kind of disease-resistant rapid-result perch cultural method and its application | |
| CN1070945A (en) | The method of induced Japanese oyster producing triploid | |
| CN1736176A (en) | Technique for producing sargassum thunbeergii kuntze offspring | |
| CN106688970A (en) | Method for artificially breeding fingerlings of siganus vulpinus in indoor environments | |
| CN101790967B (en) | Culture method of early-breeding seedlings of Charybdis japonica | |
| CN1444848A (en) | Industrial breeding method for bevel-band rockfish | |
| CN103834569B (en) | A kind of micro-algae Concentrated culture fluids and application thereof | |
| CN113951194A (en) | Method for producing triploid oysters by interspecific hybridization of Fujian oysters and sikomu | |
| CN105994026B (en) | A kind of cultural method improving freshwater shrimp commercial specification and commodity rate | |
| CN116584417A (en) | High-quality grass carp slimming cultivation method | |
| JP2023101510A (en) | Production method of natural hot spring concentrate | |
| CN105850820A (en) | Efficient lobster farming method | |
| Tanu et al. | Production and quality of mantle tissue transplanted pearls in Lamellidens marginalis cultured in different locations of Mymensingh district, Bangladesh. | |
| CN103478056B (en) | Method for improving emission ratio of pinctada maxima parent gamete | |
| CN112586413A (en) | Golden yellow seawater pearl cultivation method | |
| CN104472399B (en) | A kind of swamp eel intraspecific hybridization breeding method | |
| Amouroux | Comparative study of the carbon cycle in Venus verrucosa fed on bacteria and phytoplankton: II. Consumption of phytoplankton (Pavlova lutheri) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |