CN107085061B - 基于hplc-ms/ms检测平台的溶血磷脂酰胆碱的绝对定量分析方法 - Google Patents
基于hplc-ms/ms检测平台的溶血磷脂酰胆碱的绝对定量分析方法 Download PDFInfo
- Publication number
- CN107085061B CN107085061B CN201710372176.3A CN201710372176A CN107085061B CN 107085061 B CN107085061 B CN 107085061B CN 201710372176 A CN201710372176 A CN 201710372176A CN 107085061 B CN107085061 B CN 107085061B
- Authority
- CN
- China
- Prior art keywords
- lpc
- sample
- solution
- methanol
- formic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710372176.3A CN107085061B (zh) | 2017-05-22 | 2017-05-22 | 基于hplc-ms/ms检测平台的溶血磷脂酰胆碱的绝对定量分析方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710372176.3A CN107085061B (zh) | 2017-05-22 | 2017-05-22 | 基于hplc-ms/ms检测平台的溶血磷脂酰胆碱的绝对定量分析方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107085061A CN107085061A (zh) | 2017-08-22 |
CN107085061B true CN107085061B (zh) | 2019-03-15 |
Family
ID=59608869
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710372176.3A Active CN107085061B (zh) | 2017-05-22 | 2017-05-22 | 基于hplc-ms/ms检测平台的溶血磷脂酰胆碱的绝对定量分析方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107085061B (zh) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107589196B (zh) * | 2017-11-02 | 2019-09-20 | 浙江高美生物科技有限公司 | 一种预示肺癌脑转移的小分子标志物及在诊断方面的应用 |
CN108020592B (zh) * | 2017-11-03 | 2019-10-08 | 中国科学院武汉物理与数学研究所 | 一种毛细管电泳的质谱联用定量分析血清中磷脂酰胆碱的方法及应用 |
CN107957467B (zh) * | 2017-12-13 | 2021-02-26 | 上海景峰制药有限公司 | 一种分离测定药物制剂中溶血磷脂酰胆碱的方法 |
CN109164194A (zh) * | 2018-11-02 | 2019-01-08 | 质谱生物科技有限公司 | 干血片中溶血磷脂酰胆碱类的检测方法及其试剂盒 |
CN111435132A (zh) * | 2019-01-15 | 2020-07-21 | 武汉艾迪康医学检验所有限公司 | 溶血磷脂酰胆碱检测试剂盒和检测方法 |
CN110470764A (zh) * | 2019-08-30 | 2019-11-19 | 天津云检医学检验所有限公司 | 一种脂类的半定量分析方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106093227A (zh) * | 2016-06-01 | 2016-11-09 | 辽宁润生康泰生物医药科技有限公司 | 一种高通量检测生物体血液样本中113种脂质的液质联用方法 |
CN106353498A (zh) * | 2016-08-25 | 2017-01-25 | 邓友平 | 辅助诊断乳腺癌的脂质化合物及其相关试剂盒 |
-
2017
- 2017-05-22 CN CN201710372176.3A patent/CN107085061B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106093227A (zh) * | 2016-06-01 | 2016-11-09 | 辽宁润生康泰生物医药科技有限公司 | 一种高通量检测生物体血液样本中113种脂质的液质联用方法 |
CN106353498A (zh) * | 2016-08-25 | 2017-01-25 | 邓友平 | 辅助诊断乳腺癌的脂质化合物及其相关试剂盒 |
Non-Patent Citations (4)
Title |
---|
Lipid and lipid mediator profiling of human synovial fluid in rheumatoid arthritis patients by means of LC–MS/MS;Martin Giera 等;《Biochimica et Biophysica Acta》;20120624;第1821卷;1415-1424 |
Measurement of endogenous lysophosphatidic acid by ESI-MS/MS in plasma samples requires pre-separation of lysophosphatidylcholine;Zhenwen Zhao 等;《Journal of Chromatography B》;20090827;第877卷;摘要,第2小节 |
基于液相色谱与质谱联用的代谢组学及磷脂轮廓分析在糖代谢异常研究中的应用;赵素敏 等;《色谱》;20110430;第29卷(第4期);307-313 |
液相色谱质谱用于卵巢肿瘤中磷脂轮廓的分析;赵素敏 等;《色谱》;20110930;第29卷(第9期);843-850 |
Also Published As
Publication number | Publication date |
---|---|
CN107085061A (zh) | 2017-08-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107085061B (zh) | 基于hplc-ms/ms检测平台的溶血磷脂酰胆碱的绝对定量分析方法 | |
Yin et al. | Current state-of-the-art of nontargeted metabolomics based on liquid chromatography–mass spectrometry with special emphasis in clinical applications | |
Mullard et al. | A new strategy for MS/MS data acquisition applying multiple data dependent experiments on Orbitrap mass spectrometers in non-targeted metabolomic applications | |
Mittal | Tandem mass spectroscopy in diagnosis and clinical research | |
El-Khoury et al. | A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized L-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges | |
Rochat et al. | The future key role of LC–high-resolution-MS analyses in clinical laboratories: a focus on quantification | |
US20190346455A1 (en) | Mass spectrometry assay method for detection and quantitation of kidney function metabolites | |
Zhou et al. | The spectra count label-free quantitation in cancer proteomics | |
CN105675788A (zh) | 高效液相色谱串联质谱技术检测唾液中孕酮和睾酮的方法及试剂盒 | |
CN104364641A (zh) | 血清及其它生物基质中的分析物的量化 | |
CN105891364A (zh) | 高效液相色谱串联质谱技术检测唾液中褪黑素的方法及试剂盒 | |
Wagner et al. | Metabolite structure analysis by high-resolution MS: supporting drug-development studies | |
Dekker et al. | A new method to analyze matrix‐assisted laser desorption/ionization time‐of‐flight peptide profiling mass spectra | |
Thompson et al. | An enhanced isotopic fine structure method for exact mass analysis in discovery metabolomics: FIA-CASI-FTMS | |
US20160195502A1 (en) | Method for chiral separation of methamphetamine and amphatamine enantiomers | |
Leuthold et al. | Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip‐based infusion | |
Leitner et al. | SnapShot: mass spectrometry for protein and proteome analyses | |
Feng et al. | Dynamic binning peak detection and assessment of various lipidomics liquid chromatography-mass spectrometry pre-processing platforms | |
Zhou et al. | Performance metrics for evaluating system suitability in liquid chromatography—Mass spectrometry peptide mass mapping of protein therapeutics and monoclonal antibodies | |
Zhou et al. | Development of a high-coverage metabolome relative quantitative method for large-scale sample analysis | |
JP2020512559A (ja) | 包括的および定量的な脂質およびトコフェロールの分析 | |
Lin et al. | Joint MS‐based platforms for comprehensive comparison of rat plasma and serum metabolic profiling | |
Sharma et al. | A laconic review on liquid chromatography mass spectrometry (LC-MS) based proteomics technology in drug discovery | |
CN104991027B (zh) | 降低lc‑ms测试物中不挥发性缓冲盐含量的方法 | |
Hernández et al. | Fast determination of toxic diethylene glycol in toothpaste by ultra-performance liquid chromatography–time of flight mass spectrometry |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhang Lei Inventor after: Liu Shuye Inventor after: Fan Zhijuan Inventor after: Kang Hua Inventor after: Wang Yufan Inventor after: Tian Yaqiong Inventor after: Liu Shuang Inventor after: Wu Yujing Inventor before: Liu Shuye Inventor before: Zhang Lei Inventor before: Fan Zhijuan Inventor before: Kang Hua Inventor before: Wang Yufan Inventor before: Tian Yaqiong Inventor before: Liu Shuang Inventor before: Wu Yujing |