CN107075461A - 多杀菌素异源表达菌株及其构建方法和应用 - Google Patents

多杀菌素异源表达菌株及其构建方法和应用 Download PDF

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CN107075461A
CN107075461A CN201480083229.4A CN201480083229A CN107075461A CN 107075461 A CN107075461 A CN 107075461A CN 201480083229 A CN201480083229 A CN 201480083229A CN 107075461 A CN107075461 A CN 107075461A
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gene cluster
nucleic acid
plasmid
sequence
spinosyn
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CN107075461B (zh
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黄隽
余贞
李美红
郑玲辉
李娜
王海彬
白骅
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Zhejiang Hisun Pharmaceutical Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
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  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

提供了一种多杀菌素异源表达菌株的构建方法，通过该方法获得的多杀菌素异源表达菌株及其在制备多杀菌素中的应用。方法利用多次同源重组，将红色糖多胞菌的红霉素合成基因簇替换为多杀菌素合成基因簇和鼠李糖合成基因簇，使红色糖多孢菌表达多杀菌素。

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PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201480083229.4A 2014-11-14 2014-11-14 多杀菌素异源表达菌株及其构建方法和应用 Active CN107075461B (zh)

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PCT/CN2014/091118 WO2016074221A1 (zh) 2014-11-14 2014-11-14 多杀菌素异源表达菌株及其构建方法和应用

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CN107075461A true CN107075461A (zh) 2017-08-18
CN107075461B CN107075461B (zh) 2020-10-23

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US (1) US10844415B2 (zh)
EP (1) EP3219788A4 (zh)
JP (1) JP6641366B2 (zh)
CN (1) CN107075461B (zh)
BR (1) BR112017010073A2 (zh)
WO (1) WO2016074221A1 (zh)

Families Citing this family (9)

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Publication number Priority date Publication date Assignee Title
CN106167815A (zh) * 2016-05-30 2016-11-30 浙江农林大学 天维菌素衍生物的制备方法及用途
CN107523598B (zh) * 2017-06-20 2021-04-09 上海农乐生物制品股份有限公司 一种提高多杀菌素产量的发酵方法
CN110029069B (zh) * 2019-04-29 2022-11-29 湖南师范大学 一株浅黄霉素基因簇敲除的须糖多孢菌工程菌株及其应用
CN110713964B (zh) * 2019-04-30 2022-08-30 湖南师范大学 一株cspA基因加倍的须糖多孢菌工程菌株及其应用
CN111454975B (zh) * 2020-04-17 2023-06-20 长沙微智生物科技有限公司 一种与多杀菌素相关的ech基因的应用
CN114717281B (zh) * 2021-01-06 2023-12-08 武汉大学 通过优化碳源提升异源多杀菌素表达菌株发酵产量的方法
CN113637619B (zh) * 2021-07-23 2023-06-09 湖南师范大学 一株须糖多孢菌ΔClu13-MmsA及其构建方法与应用
CN114015604B (zh) * 2021-11-10 2023-12-15 河北兴柏农业科技股份有限公司 一种发酵培养基及发酵生产多杀菌素的方法
CN113999868A (zh) * 2021-12-06 2022-02-01 齐鲁制药（内蒙古）有限公司 一种高产多杀菌素j/l的工程菌及其构建方法与应用

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EP1032679A2 (fr) 1997-07-25 2000-09-06 Hoechst Marion Roussel Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation
US6143526A (en) * 1998-03-09 2000-11-07 Baltz; Richard H. Biosynthetic genes for spinosyn insecticide production
DE60140936D1 (de) * 2000-04-13 2010-02-11 Biotica Tech Ltd Glycosylierte hybrid-verbindungen, deren herstellung und verwendung
JP4765032B2 (ja) 2002-02-19 2011-09-07 ダウ・アグロサイエンス・エル・エル・シー 新規スピノシンを産生するポリケチド合成酵素
CN103740631B (zh) * 2013-12-31 2015-09-30 天津大学 能提高多杀菌素产量的基因工程菌及构建方法及应用

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WO2016074221A1 (zh) 2016-05-19
EP3219788A1 (en) 2017-09-20
JP6641366B2 (ja) 2020-02-05
CN107075461B (zh) 2020-10-23
EP3219788A4 (en) 2018-05-23
JP2017536820A (ja) 2017-12-14
BR112017010073A2 (zh) 2018-05-15
US20180016610A1 (en) 2018-01-18
US10844415B2 (en) 2020-11-24

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