CN107041326A - Double fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding methods - Google Patents
Double fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding methods Download PDFInfo
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- CN107041326A CN107041326A CN201710305216.2A CN201710305216A CN107041326A CN 107041326 A CN107041326 A CN 107041326A CN 201710305216 A CN201710305216 A CN 201710305216A CN 107041326 A CN107041326 A CN 107041326A
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- 241000242583 Scyphozoa Species 0.000 title claims abstract description 130
- 241000196324 Embryophyta Species 0.000 title claims abstract description 40
- 238000009395 breeding Methods 0.000 title claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 141
- 241000700141 Rotifera Species 0.000 claims abstract description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 14
- 241000239250 Copepoda Species 0.000 claims abstract description 12
- 241001247197 Cephalocarida Species 0.000 claims abstract description 10
- 230000009027 insemination Effects 0.000 claims abstract description 7
- 239000013535 sea water Substances 0.000 claims description 20
- 241000595940 Notostraca Species 0.000 claims description 18
- 239000002689 soil Substances 0.000 claims description 15
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 239000004568 cement Substances 0.000 claims description 7
- 210000004081 cilia Anatomy 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 238000004880 explosion Methods 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 7
- 210000004907 gland Anatomy 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 230000001568 sexual effect Effects 0.000 claims description 7
- 241000894007 species Species 0.000 claims description 7
- 230000029052 metamorphosis Effects 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 230000034303 cell budding Effects 0.000 claims description 5
- 230000018109 developmental process Effects 0.000 claims description 5
- 241001064683 Cladoceras Species 0.000 claims description 4
- 241000243251 Hydra Species 0.000 claims description 2
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 claims description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 claims 1
- 240000005373 Panax quinquefolius Species 0.000 claims 1
- 210000002149 gonad Anatomy 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 230000002710 gonadal effect Effects 0.000 abstract 1
- 230000035800 maturation Effects 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 description 8
- 238000006213 oxygenation reaction Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000700108 Ctenophora <comb jellyfish phylum> Species 0.000 description 1
- 241000243320 Hydrozoa Species 0.000 description 1
- 241001455233 Obelia Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Mushroom Cultivation (AREA)
- Hydroponics (AREA)
Abstract
Double fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding methods, introduced a fine variety naturally including embryonated egg, the cultivation of hydranth, jellyfish ephyra and adult, match introduces a collection, artificial insemination is expanded numerous, feed the water rotifer that heals up, Copepods and the small-sized crust zooplankter of cladocera it is characterized in that being more than using the jellyfish Metamorphore in the natural brackish water of Hangzhou Wan in salinity 6~15,29~30 DEG C of temperature, Dissolved Oxygen in Water under the conditions of 5mg/L and cultivate to adult, therefrom select double fork shallow lakes overgrown with wild plants worm jellyfish of visible gonad as parent；It is that 30rpm mechanical homogenisers are 1 sex ration using rotating speed：1~1：The jellyfish mixing of 3 gonadal maturation blends carry out artificial insemination；Incubating oosperm development obtains jellyfish adult.The demand that the present invention can preferably meet the scientific research of double fork shallow lake overgrown with wild plants branch worm jellyfishes and view and admire.
Description
Technical field
The present invention relates to jellyfish culture technique, the cultural method of especially double fork shallow lake overgrown with wild plants branch worm jellyfishes.
Background technology
Double fork shallow lake overgrown with wild plants branch worm jellyfishes (Obelia genicutata Linnaeus) belong to Coelenterata Hydrozoa, are China
A kind of wider small-sized hydromedusan of coastal distribution, occurs rich with the fluctuation of temperature, bait and salinity in different seasons
Spend difference.Branch worm jellyfishes can survive in salinity 6~15,16~34 DEG C of water bodys of temperature for double fork shallow lakes overgrown with wild plants, diameter up to more than 35mm,
Average life span was up to 3~4 months, compared with ocean large jellyfish in aquarium, and the salinity that it is required is low, food ration is few, raises
Equipment is cheap and itself purchasing price is also quite economical, and these advantages can allow double fork shallow lake overgrown with wild plants branch worm jellyfishes by suitable jellyfish
Cylinder is come into multifarious Shui nationality fan family.Double fork shallow lake overgrown with wild plants branch worm jellyfishes are warm band jellyfish, domestic at present to double fork shallow lakes overgrown with wild plants branch worm jellyfish
Breed research and have no report.For the double fork shallow lake overgrown with wild plants branch worm jellyfishes of propagation in scale, now double fork shallow lakes overgrown with wild plants branch worm jellyfish is carried out breeding research, with
This forms the breeding method of system.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of double fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding methods, solution was bred
Cheng Zhongshuan fork shallow lake overgrown with wild plants branch worm jellyfish embryonated egg introduce a fine variety, parent culture, artificial insemination, hatching, Xi shapes body budding and jellyfish ephyra
And the cultivating and managing problem of adult.
Technical scheme includes double fork shallow lakes overgrown with wild plants branch worm jellyfish embryonated eggs and introduces a fine variety, parent culture, artificial insemination, incubates naturally
Change, Xi shapes body adheres to the cultivating and managing of budding, jellyfish ephyra and adult, it is characterized in that introducing natural from Hangzhou Wan Hekou District
Brackish water is into indoor cement pit, and the jellyfish Metamorphore that water body is rich in also enters cultivation system, daytime light intensity regulate and control to 100~
600Lx, water salinity is regulated and controled to 6~15, temperature adjusting to 29~30 DEG C, Dissolved Oxygen in Water and is more than 5mg/L, according to jellyfish generation
Water rotifer, the fairy shrimp young, Copepods and the small-sized crust zooplankter of cladocera are fed successively for different phase, until jellyfish
Metamorphore development by metamorphosis is jellyfish adult, the jellyfish adult of cultivation is carried out after species differentiates and select, with the bolting silk net of 200 mesh
The soft double fork shallow lakes overgrown with wild plants branch worm jellyfish adult fished in water body is put into the bucket for filling synthermal same salinity fresh seawater, and density is not
80/L must be higher than, be quickly transferred to concentrate in synthermal same salinity seawater parent culture pond and cultivate, it is aerobic if long distance transportation
Airbag oxygenation transport, feeds 3 water rotifers, Copepods and cladocera daily, every 2 days soil pick-ups 1 time, changes water 1/3 within every 2 days, directly
It is ripe to jellyfish sexual gland；When the presentation of jellyfish gonad is faint yellow, micro- sem observation jellyfish egg cell and germ cell development are utilized
Degree, when 20%-50% mature eggs cell free is in the double fork shallow lakes overgrown with wild plants branch worm jellyfish parent in subgenital porticus, selecting Zhi Jing≤30mm
And match male and female jellyfish 1：1~1：3, it is then that 30rpm mechanical homogenisers stir the male and female jellyfish mixing matched using rotating speed
It is broken smart ovum is fully combined, quickly mixed liquor is uniformly splashed into hatchery, abundant gas explosion is mixed, and makes Dissolved Oxygen in Water concentration
It is maintained at more than 5mg/L；100~600Lx of indoor light intensity on daytime, embryonated egg passes through in salinity 6~15,29~30 DEG C of water bodys of temperature
6~12h hatchings turn into the Metamorphore with cilium, and being put into the corallite net bag being exposed to the sun in hatchery makes the hydra young attached
, respectively feed within first 5 days appropriate fresh and alive water rotifer sooner or later daily and neither add water nor change water for 1 time, the 6th day starts daily in the morning, afternoon and evening
Respectively feed the appropriate fresh and alive fairy shrimp young and change water 1/3 for 1 time, every 2 days, every 2 days soil pick-ups 1 time, Xi shapes body is cultivated through 6~8d has developed
It is kind, until hydranth produces medusa bud and forms double fork shallow lake overgrown with wild plants branch worm jellyfish ephyras, to further speed up hydranth transverse fissure speed,
Change water to be adjusted to change water 3/4 daily and change 2 DEG C of the temperature reduction of water, salinity keeps constant；When hatchery, jellyfish ephyra reaches
During to 50~60/L, the corallite net bag that hydranth adheres to is moved into new hatchery, to the jellyfish ephyra of acquisition
Continue to cultivate, change within every 2 days water 1/3, every 2 days soil pick-ups 1 time feed respectively feed 1 seawater Copepods and cladocera daily in the morning, afternoon and evening,
It can be listed when jellyfish diameter reaches more than 20mm.
The present invention fruitfully accelerates breeding speed, adding amplification scale for double fork shallow lake overgrown with wild plants branch worm jellyfishes, so that compared with
The population quantity and individual specification of the fast double fork shallow lake overgrown with wild plants branch worm jellyfishes of increase, can preferably meet the scientific research and sight of double fork shallow lake overgrown with wild plants branch worm jellyfishes
The demand of reward, for family Shui nationality market provide it is a kind of it is small-sized be easy to get view and admire jellyfish kind.
Embodiment
Specific embodiments of the present invention are described with reference to embodiment：
Embodiment 1：Annual 4~July, when Hangzhou Wan Hekou District water temperature not yet reaches 29 DEG C, with Hangzhou Wan natural half
Salt water is introduced into indoor cement pit, and water body has been also enriched in jellyfish Metamorphore, and daytime, light intensity regulated and controled to 100~600Lx, regulated and controled water
Matter index make water salinity 6~15,29~30 DEG C of temperature, Dissolved Oxygen in Water be more than 5mg/L, according to jellyfish generation different phase according to
It is secondary to feed water rotifer, the fairy shrimp young, Copepods and the small-sized crust zooplankter of cladocera, until jellyfish Metamorphore is abnormal
Develop for jellyfish adult.The jellyfish adult of cultivation is carried out after species differentiates and select, water body is softly fished for the bolting silk net of 200 mesh
In double fork shallow lakes overgrown with wild plants branch worm jellyfish adult be put into the water for filling in synthermal same salinity fresh seawater (density must not be higher than 80/L)
In bucket (the aerobic airbag oxygenation transport if long distance transportation), quickly it is transferred in synthermal same salinity seawater parent culture pond and concentrates
Cultivate, feed 3 fairy shrimp young in right amount daily, every 2 days soil pick-ups 1 time change water 1/3 in every 2 days, until by jellyfish cultivate sexual gland into
It is ripe.Its sex of precise Identification under microscope, is separately raised.It is 1 to select female-male proportion：Totally 30 groups of 1 jellyfish, is put into and fills equality of temperature
The rotating speed with salinity seawater is spent in 30rpm mechanical homogenisers, blending mixing makes smart ovum fully combine, and then splashes to hatchery
In, gas explosion is fully mixed, while making dissolved oxygen concentration in water body be maintained at more than 5mg/L.Under the conditions of indoor natural light, daytime light
Strong 100~600Lx, embryonated egg is hatched through 6~12h in salinity 6~15,29~30 DEG C of water bodys of temperature turns into floating with cilium
Unrestrained larva, being put into the corallite net bag that drains of sterilization adheres to it.Respectively feed appropriate fresh and alive water rotifer 1 sooner or later daily within first 5 days
Secondary neither to add water nor change water, beginning in the 6th day respectively feeds the appropriate fresh and alive fairy shrimp young and changes water 1/ 1 time, every 2 days in the morning, afternoon and evening daily
3, Xi shape bodies are cultivated from 4 tentacle Xi shapes bodies to 8 tentacle Xi shapes bodies through 6~8d and finally develop into 10~16 tentacles, until hydranth
Produce medusa bud and form double fork shallow lakes overgrown with wild plants branch worm jellyfish ephyras, changing water to further speed up hydranth transverse fissure speed is adjusted to daily
Change water 3/4 and change 2 DEG C of the temperature reduction of water, salinity keeps constant.When hatchery jellyfish ephyra reaches 50~60/L,
The corallite net bag that hydranth adheres to is moved into new hatchery, the jellyfish ephyra of acquisition is continued to cultivate 30d, every 2
Its soil pick-up 1 time, changes water 1/3 in every 2 days, feeds 3 fresh and alive fairy shrimp young in the morning, afternoon and evening daily, jellyfish diameter is up on during 20mm
City, 400~435nm waveform LED light sources can be equipped with during listing makes jellyfish that purple is presented.
Embodiment 2：Annual 8~September part, when Hangzhou Wan Hekou District water temperature is more than 29 DEG C, with the natural brackish water of Hangzhou Wan
It is introduced into indoor cement pit, water body has been also enriched in jellyfish Metamorphore, and daytime, light intensity regulated and controled to 100~600Lx, and regulation and control water quality refers to
Mark makes water salinity 6~15, water temperature not regulate and control, Dissolved Oxygen in Water is more than 5mg/L, is fed successively according to jellyfish generation different phase
Water rotifer, the fairy shrimp young, Copepods and the small-sized crust zooplankter of cladocera, until jellyfish Metamorphore development by metamorphosis is
Jellyfish adult.The jellyfish adult of cultivation is carried out after species differentiates and select, softly to fish for double in water body with the bolting silk net of 200 mesh
Fork shallow lake overgrown with wild plants branch worm jellyfish adult is put into the bucket for filling in synthermal same salinity fresh seawater (density must not be higher than 80/L) (such as
Long distance transportation then aerobic airbag oxygenation transport), quickly it is transferred to concentrate in synthermal same salinity seawater parent culture pond and cultivates, often
It feeds 3 Copepods in right amount, every 2 days soil pick-ups 1 time, water 1/3 is changed within every 2 days, until it is ripe that jellyfish is cultivated into sexual gland.Under microscope
Its sex of precise Identification, is separately raised.It is 1 to select female-male proportion：Totally 30 groups of 2 jellyfish, is put into and fills synthermal same salinity sea
The rotating speed of water is in 30rpm mechanical homogenisers, blending mixing makes smart ovum fully combine, and then splashes into hatchery, gas explosion is filled
Divide and mix, while making dissolved oxygen concentration in water body be maintained at more than 5mg/L.Under the conditions of indoor natural light, daytime light intensity 100~
600Lx, embryonated egg is hatched through 6~12h in salinity 6~15,29~30 DEG C of water bodys of temperature turns into the Metamorphore with cilium,
Being put into the corallite net bag that drains of sterilization adheres to it.Appropriate fresh and alive water rotifer is respectively fed within first 5 days sooner or later daily neither to add for 1 time
Water does not also change water, and beginning in the 6th day respectively feeds the appropriate fresh and alive fairy shrimp young and changes water 1/3, Xi shape bodies 1 time, every 2 days in the morning, afternoon and evening daily
Cultivated through 6~8d from 4 tentacle Xi shapes bodies to 8 tentacle Xi shapes bodies and finally develop into 10~16 tentacles, until hydranth produces jellyfish
Bud forms double fork shallow lakes overgrown with wild plants branch worm jellyfish ephyras, and changing water to further speed up hydranth transverse fissure speed is adjusted to change water 3/4 daily
And it is constant to change 2 DEG C of salinity holdings of temperature reduction of water.When hatchery jellyfish ephyra reaches 50~60/L, by hydranth
The corallite net bag of attachment is moved into new hatchery, and the jellyfish ephyra of acquisition is continued to cultivate 30d, every 3 days soil pick-ups 1
It is secondary, change water 1/3 within every 3 days, feed 3 fresh and alive Copepods in the morning, afternoon and evening daily, jellyfish diameter can be listed when reaching 20mm, can during listing
Make jellyfish that blueness is presented to be equipped with 450~480nm waveform LED light sources.
Embodiment 3：Annual 10~November, when Hangzhou Wan Hekou District water temperature is less than 29 DEG C, as Hangzhou Wan natural half is salty
Water is introduced into indoor cement pit, and water body has been also enriched in jellyfish Metamorphore, and daytime, light intensity regulated and controled to 100~600Lx, regulated and controled water quality
Index makes water salinity 6~15,29~30 DEG C of temperature, Dissolved Oxygen in Water be more than 5mg/L, according to jellyfish generation different phase successively
Water rotifer, the fairy shrimp young, Copepods and the small-sized crust zooplankter of cladocera are fed, until jellyfish Metamorphore metamorphosis hair
Educate for jellyfish adult.The jellyfish adult of cultivation is carried out after species differentiates and select, softly to be fished in water body with the bolting silk net of 200 mesh
Double fork shallow lakes overgrown with wild plants branch worm jellyfish adult be put into the bucket for filling in synthermal same salinity fresh seawater (density must not be higher than 100/L)
In (if long distance transportation aerobic airbag oxygenation transport), be quickly transferred in synthermal same salinity seawater parent culture pond and concentrate training
Educate, feed 3 cladoceras in right amount daily, every 2 days soil pick-ups 1 time change water 1/3 in every 2 days, until it is ripe that jellyfish is cultivated into sexual gland.It is aobvious
Its sex of precise Identification under micro mirror, is separately raised.It is 1 to select female-male proportion：Totally 30 groups of 3 jellyfish, be put into fill it is synthermal same
The rotating speed of salinity seawater be 30rpm mechanical homogenisers in, blending mixing makes smart ovum fully combine, and then splashes into hatchery,
Gas explosion is fully mixed, while making dissolved oxygen concentration in water body be maintained at more than 5mg/L.Under the conditions of indoor natural light, daytime light intensity
100~600Lx, embryonated egg is hatched through 6~12h in salinity 6~15,29~30 DEG C of water bodys of temperature turns into the floating wave with cilium
Larva, being put into the corallite net bag that drains of sterilization adheres to it.Respectively feed within first 5 days appropriate fresh and alive water rotifer sooner or later daily 1 time
Neither add water nor change water, beginning in the 6th day respectively feeds the appropriate fresh and alive fairy shrimp young and changes water 1/3 1 time, every 2 days in the morning, afternoon and evening daily,
Xi shapes body is cultivated from 4 tentacle Xi shapes bodies to 8 tentacle Xi shapes bodies through 6~8d and finally develops into 10~16 tentacles, until hydranth production
Raw medusa bud forms double fork shallow lakes overgrown with wild plants branch worm jellyfish ephyras, and changing water to further speed up hydranth transverse fissure speed is adjusted to change daily
Water 3/4 and change the temperature of water and reduce by 2 DEG C of salinity and keep constant., will when hatchery jellyfish ephyra reaches 50~60/L
The corallite net bag of hydranth attachment is moved into new hatchery, and the jellyfish ephyra of acquisition is continued to cultivate 30d, every 3 days
Soil pick-up 1 time, changes water 1/2 in every 7 days, feeds 3 fresh and alive cladoceras in the morning, afternoon and evening daily, can be listed when jellyfish diameter is up to 20mm,
500~560nm waveform LED light sources can be equipped with during listing makes jellyfish that green is presented.
Claims (3)
1. pair fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding method, including double fork shallow lakes overgrown with wild plants branch worm jellyfish embryonated egg introduce a fine variety naturally, parent culture, people
Work insemination, hatching, Xi shapes body attachment budding, the cultivating and managing of jellyfish ephyra and adult, it is characterized in that annual 4~July,
When Hangzhou Wan Hekou District water temperature not yet reaches 29 DEG C, as the natural brackish water of Hangzhou Wan is introduced into indoor cement pit, water body is also rich
Jellyfish Metamorphore is contained, daytime, light intensity regulated and controled to 100~600Lx, and regulation and control water quality index makes water salinity 6~15, temperature 29
~30 DEG C, Dissolved Oxygen in Water be more than 5mg/L, water rotifer, the fairy shrimp young, oar are fed according to jellyfish generation different phase successively
Sufficient class and the small-sized crust zooplankter of cladocera, until the development by metamorphosis of jellyfish Metamorphore is jellyfish adult；To the jellyfish of cultivation
After adult progress species discriminating is selected, the branch worm jellyfish adult of double fork shallow lakes overgrown with wild plants in water body is softly fished for the bolting silk net of 200 mesh and is put into
In the bucket for filling synthermal same salinity fresh seawater, density must not be higher than 80/L, quickly be transferred to synthermal same salinity seawater
Concentrate and cultivate in parent culture pond, feed 3 fairy shrimp young daily, every 2 days soil pick-ups 1 time change water 1/3 in every 2 days, until by water
It is ripe that mother cultivates sexual gland；Its sex of precise Identification under microscope, is separately raised, and it is 1 to select female-male proportion：Totally 30 groups of 1 jellyfish,
It is put into and fills the rotating speed of synthermal same salinity seawater in 30rpm mechanical homogenisers, blending mixing makes smart ovum fully combine, then
Splash into hatchery, gas explosion is fully mixed, while making dissolved oxygen concentration in water body be maintained at more than 5mg/L；Indoor natural light
Under the conditions of, daytime 100~600Lx of light intensity, embryonated egg is hatched into through 6~12h in salinity 6~15,29~30 DEG C of water bodys of temperature
For the Metamorphore with cilium, being put into the corallite net bag that sterilization drains adheres to it；Respectively feed sooner or later daily within first 5 days appropriate
Fresh and alive water rotifer neither adds water nor changes water for 1 time, starts within the 6th day respectively to feed the appropriate fresh and alive fairy shrimp young 1 in the morning, afternoon and evening daily
Water 1/3 is changed within secondary, every 2 days, Xi shapes body finally develops into 10~16 from 4 tentacle Xi shapes bodies to 8 tentacle Xi shapes bodies through 6~8d cultivations and touched
Hand, until hydranth produces medusa bud and forms double fork shallow lake overgrown with wild plants branch worm jellyfish ephyras, to further speed up hydranth transverse fissure speed
Changing water and being adjusted to change water 3/4 daily and change the temperature of water reduces by 2 DEG C of salinity and keeps constant；When hatchery, jellyfish ephyra reaches
During 50~60/L, the corallite net bag that hydranth is adhered to is moved into new hatchery, to the jellyfish ephyra of acquisition after
Continuous to cultivate 30d, every 2 days soil pick-ups 1 time change water 1/3 in every 2 days, feed 3 fresh and alive fairy shrimp young, jellyfish diameter in the morning, afternoon and evening daily
Listed during up to 20mm.
2. pair fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding method, including double fork shallow lakes overgrown with wild plants branch worm jellyfish embryonated egg introduce a fine variety naturally, parent culture, people
Work insemination, hatching, Xi shapes body attachment budding, the cultivating and managing of jellyfish ephyra and adult, it is characterized in that annual 8~September part,
When Hangzhou Wan Hekou District water temperature is more than 29 DEG C, as the natural brackish water of Hangzhou Wan is introduced into indoor cement pit, water body is also enriched in
Jellyfish Metamorphore, daytime, light intensity regulated and controled to 100~600Lx, regulation and control water quality index makes water salinity 6~15, water temperature not regulate and control,
Dissolved Oxygen in Water be more than 5mg/L, according to jellyfish generation different phase feed successively water rotifer, the fairy shrimp young, Copepods and
The small-sized crust zooplankter of cladocera, until the development by metamorphosis of jellyfish Metamorphore is jellyfish adult；The jellyfish adult of cultivation is entered
Row species differentiate select after, softly fished for the bolting silk net of 200 mesh in water body double fork shallow lakes overgrown with wild plants branch worm jellyfish adult be put into fill together
In bucket of the temperature with salinity fresh seawater, density must not be higher than 80/L, quickly be transferred to synthermal same salinity seawater parent training
Educate to concentrate in pond and cultivate, feed 3 Copepods daily, every 2 days soil pick-ups 1 time change water 1/3 in every 2 days, until jellyfish is cultivated into sexual gland
It is ripe；Its sex of precise Identification under microscope, is separately raised, and it is 1 to select female-male proportion：Totally 30 groups of 2 jellyfish, is put into and fills together
Temperature with salinity seawater rotating speed be 30rpm mechanical homogenisers in, blending mixing makes smart ovum fully combine, and then splashes to hatching
Chi Zhong, gas explosion is fully mixed, while making dissolved oxygen concentration in water body be maintained at more than 5mg/L；Under the conditions of indoor natural light, daytime
100~600Lx of light intensity, embryonated egg is hatched through 6~12h and turned into cilium in salinity 6~15,29~30 DEG C of water bodys of temperature
Metamorphore, being put into the corallite net bag that drains of sterilization adheres to it；Respectively feed appropriate fresh and alive water rotifer sooner or later daily within first 5 days
Neither add water nor change water for 1 time, start within the 6th day respectively to feed the appropriate fresh and alive fairy shrimp young in the morning, afternoon and evening daily and change water 1 time, every 2 days
1/3, Xi shape body is cultivated from 4 tentacle Xi shapes bodies to 8 tentacle Xi shapes bodies through 6~8d and finally develops into 10~16 tentacles, until hydra
Body produces medusa bud and forms double fork shallow lakes overgrown with wild plants branch worm jellyfish ephyras, and changing water to further speed up hydranth transverse fissure speed is adjusted to every
It changes water 3/4 and changes the temperature of water and reduces by 2 DEG C of salinity and keep constant；When hatchery, jellyfish ephyra reaches 50~60/L
When, the corallite net bag that hydranth adheres to is moved into new hatchery, the jellyfish ephyra of acquisition is continued to cultivate 30d,
Every 3 days soil pick-ups 1 time, change water 1/3 in every 3 days, feed 3 fresh and alive Copepods in the morning, afternoon and evening daily, and jellyfish diameter is listed when reaching 20mm.
3. pair fork shallow lake overgrown with wild plants branch worm jellyfish artificial breeding method, including double fork shallow lakes overgrown with wild plants branch worm jellyfish embryonated egg introduce a fine variety naturally, parent culture, people
Work insemination, hatching, Xi shapes body attachment budding, the cultivating and managing of jellyfish ephyra and adult, it is characterized in that annual 10~November
Part, when Hangzhou Wan Hekou District water temperature is less than 29 DEG C, as the natural brackish water of Hangzhou Wan is introduced into indoor cement pit, water body is also enriched in
Jellyfish Metamorphore, daytime, light intensity regulated and controled to 100~600Lx, regulation and control water quality index make water salinity 6~15, temperature 29~
30 DEG C, Dissolved Oxygen in Water be more than 5mg/L, according to jellyfish generation different phase feed successively water rotifer, the fairy shrimp young, oar foot
Class and the small-sized crust zooplankter of cladocera, until the development by metamorphosis of jellyfish Metamorphore is jellyfish adult；To the jellyfish of cultivation into
After body progress species discriminating is selected, the branch worm jellyfish adult of double fork shallow lakes overgrown with wild plants in water body is softly fished for the bolting silk net of 200 mesh and is put into Sheng
In the bucket for having synthermal same salinity fresh seawater, density must not be higher than 100/L, quickly be transferred to synthermal same salinity seawater parent
Concentrate and cultivate in this culture pond, feed 3 cladoceras in right amount daily, every 2 days soil pick-ups 1 time change water 1/3 in every 2 days, until by jellyfish
Cultivate sexual gland ripe；Its sex of precise Identification under microscope, is separately raised；It is 1 to select female-male proportion：Totally 30 groups of 3 jellyfish, puts
Enter to fill the rotating speed of synthermal same salinity seawater in 30rpm mechanical homogenisers, blending mixing makes smart ovum fully combine, and then sprinkles
Spill into hatchery, gas explosion is fully mixed, while making dissolved oxygen concentration in water body be maintained at more than 5mg/L；Indoor Natural striation
Under part, daytime 100~600Lx of light intensity, in salinity 6~15,29~30 DEG C of water bodys of temperature embryonated egg through 6~12h hatching turn into
Metamorphore with cilium, being put into the corallite net bag that drains of sterilization adheres to it；Respectively feed sooner or later daily within first 5 days appropriate fresh
Living water rotifer neither adds water nor changes water for 1 time, start within the 6th day respectively to feed in the morning, afternoon and evening daily the appropriate fresh and alive fairy shrimp young 1 time,
Water 1/3 is changed within every 2 days, Xi shapes body is cultivated from 4 tentacle Xi shapes bodies to 8 tentacle Xi shapes bodies through 6~8d and finally develops into 10~16 tentacles,
Until hydranth produces medusa bud and forms double fork shallow lake overgrown with wild plants branch worm jellyfish ephyras, water is changed to further speed up hydranth transverse fissure speed
Being adjusted to change water 3/4 daily and changing the temperature of water reduces by 2 DEG C of salinity and keeps constant；50 are reached when hatchery jellyfish ephyra~
During 60/L, the corallite net bag that hydranth adheres to is moved into new hatchery, the jellyfish ephyra of acquisition is continued to train
Educate 30d, every 3 days soil pick-ups 1 time change water 1/2 in every 7 days, 3 fresh and alive cladoceras fed in the morning, afternoon and evening daily, when jellyfish diameter reaches 20mm
It can list.
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| CN100444726C (en) * | 2007-04-06 | 2008-12-24 | 西华师范大学 | Artificial reproduction of peach jelly-fish |
| CN102524126A (en) * | 2012-01-05 | 2012-07-04 | 李新书 | Method for artificially propagating moon jellyfish |
| CN102907360A (en) * | 2012-10-29 | 2013-02-06 | 中国水产科学研究院黄海水产研究所 | Large-scale breeding method for white cyanea nozakii |
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| CN100444726C (en) * | 2007-04-06 | 2008-12-24 | 西华师范大学 | Artificial reproduction of peach jelly-fish |
| CN102524126A (en) * | 2012-01-05 | 2012-07-04 | 李新书 | Method for artificially propagating moon jellyfish |
| CN102907360A (en) * | 2012-10-29 | 2013-02-06 | 中国水产科学研究院黄海水产研究所 | Large-scale breeding method for white cyanea nozakii |
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| CN109892261A (en) * | 2017-12-11 | 2019-06-18 | 深圳市高盛生物科技发展有限公司 | Papua glass gall mother's artificial cultivating method |
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