CN107022507A - A kind of microbial inoculum combination of urban afforestation fermentation waste and its zymotechnique - Google Patents
A kind of microbial inoculum combination of urban afforestation fermentation waste and its zymotechnique Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention discloses a kind of combination of the microbial inoculum of urban afforestation fermentation waste and its zymotechnique.The microbial inoculum combination includes microbial inoculum 1：Actinomyces, soft rotten fungi, brown rot fungus and white-rot fungi, and suppress butyric and the growth inhibition lactic acid bacteria of spoilage organisms；Microbial inoculum 2：Heat resistance bacillus group, lactobacillus, Bifidobacteria population, photosynthetic bacteria group, yeast flora and actinomycete group；Microbial inoculum 3：Saccharomycete, photosynthetic bacteria.The zymotechnique includes urban afforestation waste material being broken into powder, and spraying water makes powder moist；Microbial inoculum 1 is added, in indoor heap fermentation a couple of days；Microbial inoculum 2 is added, aerobic rotten sour bacterium, the biological growth of butyric is prevented, continues ten a couple of days of fermenting；Add the fermentation a couple of days of microbial inoculum 3.The microbial inoculum combination of the present invention and its zymotechnique solve the process problem of urban afforestation waste material, solve pollution problem, turn waste into wealth simultaneously, and fertilizer made from this method, N-P-K content is high, is excellent organic fertilizer.
Description
Technical field
The present invention relates to bio-fermentation engineering field, the microbial inoculum of more particularly to a kind of urban afforestation fermentation waste is combined
And its zymotechnique.
Background technology
With developing rapidly for urban afforestation, urban green space expands rapidly, and urban afforestation waste material is largely produced, particularly north
The autumn and winter in mahjong city.Urban afforestation waste material includes urban trees branch, Urban fallen leaves and city grass cuttings.Traditional idea is usually
Think trees greening waste material influence health, influence landscape, substantial amounts of manpower fund is then consumed every year it is cleared up, burnt
Burn, so as to be posed a big pressure to municipal administration, burning causes very big pollution to environment.
Mainly there are 3 kinds for the processing method that main garden afforests intensively trees greening waste material at present：Sanitary landfills, burn
Burn and conventional method compost, but these methods have certain limitation：First, landfill is simple to operate, but floor space is big, and
There may be secondary pollution；2nd, investment is burned huge, and burning can produce smoke pollution problem；3rd, traditional compost method is just
Ground, which is stacked, is also easy to produce foul smell, pollutes surrounding enviroment, while the methane produced that ferments can turn into the hidden danger of fire and blast, is discharged into
Greenhouse effects can be produced in air again.Therefore, the method for finding reasonable and economic and environment-friendly processing deadwood is quite important.
This area needs the technique and method handled urban afforestation waste material.
The content of the invention
The problem of in order to solve urban afforestation waste material, the present invention provides a kind of urban afforestation fermentation waste in one aspect
Microbial inoculum combination and its zymotechnique.
Therefore, in a first aspect, the invention provides a kind of combination of the microbial inoculum of urban afforestation fermentation waste, the microbial inoculum group
Conjunction includes microbial inoculum 1：Actinomyces, soft rotten fungi, brown rot fungus, white-rot fungi and lactic acid bacteria；Microbial inoculum 2：Heat resistance bacillus group,
Lactobacillus, Bifidobacteria population, photosynthetic bacteria group, yeast flora and actinomycete group；Microbial inoculum 3：Saccharomycete, photosynthetic bacteria.
In one embodiment, according to 20-30 parts of actinomyces, soft rotten fungi 50-70 parts, it is 50-70 parts of brown rot fungus, white
Rotten fungi 50-70 parts, 20-25 parts of lactic acid bacteria；10-25 parts of heat resistance bacillus group, 10-20 parts of lactobacillus, Bifidobacterium
8-15 parts of group, 20-25 parts of photosynthetic bacteria group, 10-20 parts of yeast flora, 20-30 parts of actinomycete group；It is 20-30 parts of saccharomycete, photosynthetic thin
The weight ratio that 10-25 parts of bacterium is used.
In one embodiment, the urban afforestation waste material is the urban afforestation waste material of varieties in saline-alkali areas, such as Tian Jinbin
The urban afforestation waste material in extra large new district.
In preferred embodiments, in step 1) in, the varieties in saline-alkali areas is temperate zone semiarid zone.
In second aspect, present invention also offers a kind of fermentation process of urban afforestation waste material, the fermentation process includes：
(1) urban afforestation waste material is broken into powder, and spraying water makes powder moist；(2) microbial inoculum 1 is added, in indoor heap fermentation a couple of days, for example
5-9 days；(3) microbial inoculum 2 is added at normal temperatures, is prevented aerobic rotten sour bacterium, the biological growth of butyric, is continued ten a couple of days of fermenting,
Such as 17-19 days；(4) the fermentation a couple of days of microbial inoculum 3, such as 6-8 days are added.
In a preferred embodiment, after step (2), including (2 ') that the fermentate of acquisition is heated into 90 is Celsius
More than degree maintain 1-3 hours.
In a preferred embodiment, after step (3), including (3 ') shine the fermentate of acquisition under uviol lamp
Penetrate 1-3 hours.
In the third aspect, present invention also offers the fertilizer of the preparation of the fermentation process by second aspect of the present invention.
In the third aspect, it is used for what areal plant plait was cultivated present invention also offers the fertilizer of third aspect present invention
Purposes.
In preferred embodiments, the plant plait is Stipa grandis or Chinese pennisetum.
The microbial inoculum combination of the present invention and its zymotechnique solve the process problem of urban afforestation waste material, solve pollution and ask
Topic, turns waste into wealth simultaneously, fertilizer made from this method, and N-P-K content is high, is excellent organic fertilizer.Inventor's discovery, salt
The organic fertilizer that the urban afforestation waste material in alkali area is obtained is particularly suitable for use in the grass growth of varieties in saline-alkali areas.
The microbial inoculum combination of the urban afforestation fermentation waste of the present invention and its zymotechnique can be to producing during urban afforestation
Raw greening waste material is re-used, and is reduced pollution of the trash burning to environment, be can be widely applied for construction of city afforestation.Together
When, urban afforestation fermentation waste technique of the invention is simply easily realized, the organic matter organic principle of preparation is high, can be effectively right
Waste recycling, and the fertilizer during urban afforestation, reduce the use of chemical pesticide.
It should be appreciated that foregoing description substantially and follow-up description in detail are exemplary illustration and explanation, should not
As the limitation to claimed content of the invention.
Embodiment
The invention provides a kind of combination of the microbial inoculum of urban afforestation fermentation waste, the microbial inoculum combination includes microbial inoculum 1：Unwrapping wire
Bacterium, soft rotten fungi, brown rot fungus, white-rot fungi and lactic acid bacteria；Microbial inoculum 2：Heat resistance bacillus group, lactobacillus, bifid bar
Flora, photosynthetic bacteria group, yeast flora and actinomycete group；Microbial inoculum 3：Saccharomycete, photosynthetic bacteria.
Respectively by purchased in market (such as from Yangzhou Haicheng Bioisystech Co., Ltd, the gloomy limited public affairs of not natural product in Shaanxi
Department, high yield probiotics, Su Kehan (Weifang) bioengineering Co., Ltd, east veterinary drug Co., Ltd, Xiamen it is general biotechnology
Co., Ltd, Yangzhou Haicheng Bioisystech Co., Ltd, Cangzhou, Hebei Province Zhong Xin bio tech ltd, the green brightness in Guangzhou
Bio tech ltd etc.) actinomyces, lactic acid bacteria, heat resistance bacillus group, lactobacillus, Bifidobacteria population, photosynthetic
Flora, actinomycete group and the strain of photosynthetic bacteria carry out High Density Cultivation, are seeded to first equipped with the training that volume ratio is 25-35%
Activation culture in the shaking flask of base is supported, the formula of the activation medium is glucose 18-22g/L, peptone 12-18g/L, chlorination
Sodium 3-7g/L and beef extract 0.2-0.8g/L, is 36-39 DEG C in cultivation temperature, shaking flask rotating speed 180-220rpm cultures 20-32h.
Cultured strain is inoculated into the fermentation tank equipped with volume ratio for 50-60% fermentation medium, volume inoculum concentration is 1-
2%, carry out fermentation and expand culture, the formula of the fermentation medium is corn flour 10-15g/L, glucose 3-8g/L, beancake powder
18-22g/L, fish meal 3-8g/L, calcium carbonate 5-9g/L, ammonium sulfate 0.8-1.2g/L, dipotassium hydrogen phosphate 0.1-0.5g/L, magnesium sulfate
0.1-0.3g/L and manganese sulfate 0.1-0.3g/L, is 36-40 DEG C in cultivation temperature, fermentation tank speed of agitator 200-220rpm cultures
Content >=10 of thalline in 20-32h, fermentation tank8Individual/ml.It will be enlarged by cultivating obtained various single bacteriums and dehydrate, be prepared into not
Dormancy body microbial dry powder, then according to 20-30 parts of actinomyces, 20-25 parts of lactic acid bacteria, 10-25 parts of heat resistance bacillus group, breast
Sour flora 10-20 parts, 8-15 parts of Bifidobacteria population, 20-25 parts of photosynthetic bacteria group, 20-30 parts of actinomycete group and photosynthetic bacteria 10-25
The weight ratio of part is used.
Soft rotten fungi, brown rot fungus and white-rot fungi are voluntarily gathered in the woods of Tianjin mountain area, and collection carries disease germs wooden unit, son in fact
Body etc., is then cultivated, and the primary raw material of cultivation is to carry out drying process after the wood chip and cotton seed hulls of broad leaf tree, harvest.It is soft
Rotten fungi, brown rot fungus and white-rot fungi three use according to 50-70 parts of weight ratio.
Yeast flora and saccharomycete are obtained by purchased in market, and the two makes according to the weight ratio of 10-20 parts and 20-30 parts respectively
With.
In the present invention, urban afforestation waste material includes urban trees branch, Urban fallen leaves and city grass cuttings.
The method of the present invention after urban afforestation fermentation waste, will can play thorough " deodorization ", " becoming thoroughly decomposed ", " desinsection ", " go out
The effect of bacterium ", ferment the organic fertilizer, and not only fertilizer efficiency is good, safe and convenient to use, but also with disease-resistant growth-promoting, land productivity of fostering and apply fertilizer,
The effect such as chemical fertilizer utilization ratio is improved, is really achieved many things at one stroke, is that current processing urban afforestation waste material is most efficient method.In addition,
Also can by urban afforestation fermentation waste into high-quality seedling medium.
By reference to one exemplary embodiment, the purpose of the present invention and function and the side for realizing these purposes and function
Method will be illustrated.However, the present invention is not limited to one exemplary embodiment as disclosed below；Can by multi-form come
It is realized.The essence of specification is only to aid in the detail of the various equivalent modifications Integrated Understanding present invention.
Embodiment 1.
In the present embodiment, the raw material for preparing organic matter is all from the branch that the city of Binhai New Area in Tianjin is overhauled.
In the present embodiment, the preparation method of organic matter is：
(1) urban afforestation waste material is broken into powder as major ingredient, and spraying water makes powder moist；
(2) microbial inoculum 1 measured as follows is added in terms of every 10000 parts dry greening waste powders：20-30 parts, soft rotten fungi 50-70
Part, 50-70 parts of brown rot fungus, 50-70 parts of white-rot fungi, 20-25 parts of lactic acid bacteria, in indoor heap fermentation 5-9 days；By acquisition
Fermentate is heated to more than 90 degrees Celsius and maintained 30-60 minutes, or is irradiated 1-3 hours under uviol lamp；
(3) microbial inoculum 2 is added in terms of every 10000 parts dry greening waste powders at normal temperatures：Heat resistance bacillus group 10-25
Part, 10-20 parts of lactobacillus, 8-15 parts of Bifidobacteria population, 20-25 parts of photosynthetic bacteria group, 10-20 parts of yeast flora, actinomycete group
20-30 parts, aerobic rotten sour bacterium, the biological growth of butyric are prevented, the mixture for being used to ferment is entered in fermentation tank
Row fermentation, start-up temperature is at 20 DEG C, and fermentation calefaction control is advisable below 70-75 DEG C, ferments 17-19 days, in operation
Therefore oxygen supply measure should be increased, accomplish to mix thoroughly, duty is turned over, ventilation is advisable；The fermentate of acquisition is irradiated 1-3 hours under uviol lamp；
(4) microbial inoculum 3 is added in terms of every 10000 parts dry greening waste powders：20-30 parts of saccharomycete, 10-25 parts of hairs of photosynthetic bacteria
Ferment 6-8 days, obtains fermentate；
(5) fermentate is subjected to dewater treatment, obtains organic fertilizer.
Embodiment 2
Dewater treatment is carried out to the fermentate that embodiment 1 is obtained in the present embodiment, first, fermentate entered using water absorbing agent
Row water suction, is then separated the water absorbing agent and fermentate after water suction using microfiltration membranes.It should be appreciated that at for dehydration
Fermentate is simply reached dehydration purpose so as to obtain organic fertilizer by reason, and this does not invent the restriction being not limited to dewatering.
The organic fertilizer that the present invention is provided, wherein Source Organic Matter urban afforestation fertilizer, organic matter accounting are 68.24%.
Here is the organic fertilizer that the present invention is provided to be done in the analysis of composition, the present embodiment to prepare organic fertilizer side to the present invention
The composition for the organic fertilizer that method is obtained is analyzed by testing detection.Specifically, experiment detection project be full nitrogen, full phosphorus, full potassium,
Organic matter and pH, data are as shown in the table：
| Detection project | Measurement unit | Testing result |
| Organic matter | % (kg) | 68.24 |
| N | % (kg) | 1.395 |
| P2O5 | % (kg) | 0.676 |
| K2O | % (kg) | 0.851 |
| pH | 8.31 |
The present embodiment can be seen that the present invention by upper table and prepare the organic fertilizer that the method for organic fertilizer is obtained, and organic matter is accounted for
Than 68.24%, with higher organic principle.N P and K accounting reaches 2.92%.
Embodiment 3
The organic fertilizer of the present invention of embodiment 1 is used for Binhai New Area in Tianjin and cultivates Chinese pennisetum and Stipa grandis.Tianjin Binhai is new
Area is located in Bohai Sea Gulf, especially Tanggu, the huge port two places salinization of soil seriously, and salt content is high, and soil lacks fertility, and organic matter contains
Amount is low.
Chinese pennisetum cultural method, comprises the following steps：
1) milpa is selected：Select the broad-leaved underwood planting of varieties in saline-alkali areas city, 2-5 meters of height above sea level；Chinese pennisetum seed is selected, is sprouted
Hair rate is more than or equal to 85%；300 kgs/acre of afforestation organic fertilizer or urban afforestation fermentation waste organic fertilizer 300 are applied before broadcasting
Kg/acre, avoid trees and plough deeply soil 50cm, then carry out site preparation, soil is reached state to be broadcast；
2) site preparation is fine at the beginning of 3 months early springs the sowing time of Chinese pennisetum, and the fine earth for after planting covering 0.5 centimetre is kept
Water content 30-40% in soil, and carry out overlay film insulation, moisturizing；When temperature can surely be emerged by after planting 5-6 days.In sowing
Preceding Chinese pennisetum seed bubble irradiates 1-3 hours in the water of the hydrochloric acid containing 0.2-0.3%, and under uviol lamp.Chinese pennisetum uses drilling, mu
0.7-1.0 kilograms of the amount of broadcasting, 50 centimetres of line-spacing.300 kgs/acre of afforestation organic fertilizer or urban afforestation fermentation waste are applied during plantation
300 kgs/acre of organic fertilizer.
3) monthly watered once after emerging, water capacity is maintained at 70% to 80%；
4) to by the end of May at the beginning of 6 month, overlay film being removed when daily mean temperature reaches 18 degrees Celsius, is watered week about 1 time,
Water capacity is maintained at 70% to 80%, and fertilising is to supplement based on phosphorus potash fertilizer.Mu applies 15-20 kilograms of calcium superphosphate and makees base manure,
Every time cradle after will spraying fertilizer once, every mu using 5 kilograms of urea (or other nitrogenous fertilizer, people and animals' fecaluria) topdress.
Cradle utilization：When making greenfeed, typically cradled after jointing, Cutting Height according to raise object depending on, feeding fish,
It is almost blade full when rabbit, 70-80 centimetres of goose (or pig) plant；When feeding ox, sheep, plant height is generally 1-1.3 meters, cradles and stays
10-15 centimetres of height of stubble, never cuts, otherwise can influence again long together.Such as toothing is too high, and the branch growth occurred from nodal bud is not strengthened
Also yield can be influenceed.It should not be cradled overcast and rainy, otherwise can cause seriously to be short of seedling and the underproduction.Modulation ensilage is cut after heading
Cut, modulator approach is identical with corn.
According to test, under the same conditions, urban afforestation fermentation waste organic fertilizer can reach and apply that afforestation has
Effect as machine fertilizer, Chinese pennisetum obtains can reach 1320 kilograms and 1380 kilograms respectively per per mu yield.
Stipa grandis cultural method, comprises the following steps：
1) milpa is selected：Select the broad-leaved underwood planting of varieties in saline-alkali areas city, 2-5 meters of height above sea level；Stipa grandis seed is selected, is sprouted
Hair rate is more than or equal to 85%；300 kgs/acre of afforestation organic fertilizer or urban afforestation fermentation waste organic fertilizer are applied before broadcasting, is hided
Open trees and plough deeply soil 50cm, the river sand by terms of soil weight 20% is mixed in topsoil, site preparation is then carried out, makes soil
Reach state to be broadcast；
2) site preparation is fine in early April in early spring the sowing time of Stipa grandis, and the fine earth for after planting covering 0.5 centimetre is protected
Water content 30-40% in soil is held, and carries out overlay film insulation, moisturizing；When temperature can surely be emerged by after planting 5-6 days.Broadcasting
Stipa grandis seed bubble irradiates 1-3 hours in the water of the acetic acid containing 0.2-0.3%, and under uviol lamp before kind.Stipa grandis is used and broadcasted sowing,
0.8-1.2 kilograms of the amount of broadcasting of mu.300 kgs/acre of afforestation organic fertilizer or urban afforestation fermentation waste organic fertilizer 300 are applied during plantation
Kg/acre.
3) watered after emerging once, water capacity is maintained at 35% to 50%；
4) to by the end of April, at the beginning of 5 months, overlay film is removed when daily mean temperature reaches 15 degrees Celsius, monthly water 1 time, soil is held
Water quantity holding is 35% to 45%, and fertilising is to supplement based on phosphorus potash fertilizer；
5) gathered at the beginning of 7 the end of month to August, or the caryopsis come off is harvested at the beginning of September.
According to test, under the same conditions, Stipa grandis seed is steeped in the water of the acetic acid containing 0.2-0.3% prior to seeding, and
Irradiation can improve emergence rate for 1-3 hours up to 18% under uviol lamp.
Good effect, Stipa grandis can be reached by applying afforestation organic fertilizer or urban afforestation fermentation waste organic fertilizer
1780 kilograms and 1830 kilograms can be reached respectively per per mu yield by obtaining.
It is not intended to rigidly adhere in any theory, the organic matter that areal is obtained may contain needed for the plant growth of areal
More reasonably nutritional ingredient.
The preparation method of the organic fertilizer of the present invention can be re-used to the branch overhauled during urban afforestation, be reduced
Pollution of the trash burning to environment, can be widely applied for construction of city afforestation.Meanwhile, preparation method of organic fertilizer letter of the invention
Single easily to realize, the organic matter organic principle of preparation is high, can turn waste into wealth effectively to waste recycling.Meanwhile, the present invention
The organic fertilizer of offer reduces the use of chemical pesticide, effectively protects environment as the fertilizer during urban afforestation.
With reference to the explanation of the invention disclosed here and practice, other embodiment of the invention is for those skilled in the art
It all will be readily apparent and understand.Illustrate and embodiment is to be considered only as exemplary, of the invention true scope and purport is equal
It is defined in the claims.
Claims (10)
1. a kind of microbial inoculum combination of urban afforestation fermentation waste, the microbial inoculum combination includes microbial inoculum 1：It is actinomyces, soft rotten fungi, brown
Rotten fungi, white-rot fungi and lactic acid bacteria；Microbial inoculum 2：Heat resistance bacillus group, lactobacillus, Bifidobacteria population, photosynthetic bacteria group,
Yeast flora and actinomycete group；Microbial inoculum 3：Saccharomycete, photosynthetic bacteria.
2. the microbial inoculum combination of claim 1, the microbial inoculum 1 is according to 20-30 parts of actinomyces, soft rotten fungi 50-70 parts, brown rot fungus
50-70 parts, 50-70 parts of white-rot fungi, the weight ratio of 20-25 parts of lactic acid bacteria use.
3. the microbial inoculum combination of claim 1, the microbial inoculum 2 is according to heat resistance bacillus group 10-25 parts, lactobacillus 10-20
Part, the weight ratio of 8-15 parts of Bifidobacteria population, 20-25 parts of photosynthetic bacteria group, 10-20 parts of yeast flora, 20-30 parts of actinomycete group make
With.
4. the microbial inoculum combination of claim 1, the microbial inoculum 3 is according to 20-30 parts of saccharomycete, the weight ratio of 10-25 parts of photosynthetic bacteria
Use.
5. a kind of fermentation process of urban afforestation waste material, the fermentation process includes：
(1) urban afforestation waste material is broken into powder, and spraying water makes powder moist；
(2) microbial inoculum 1 is added, in indoor heap fermentation a couple of days；
(3) microbial inoculum 2 is added at normal temperatures, is prevented aerobic rotten sour bacterium, the biological growth of butyric, is continued ten a couple of days of fermenting；
(4) the fermentation a couple of days of microbial inoculum 3 is added.
6. the fermentation process of claim 5, after step (2), including (2 ') by the fermentate of acquisition be heated to 90 degrees Celsius with
Upper maintenance 1-3 hours.
7. the fermentation process of claim 5, after step (3), including the fermentate of acquisition is irradiated 1-3 by (3 ') under uviol lamp
Hour.
8. the fermentation process of claim 5, the urban afforestation waste material is the urban afforestation waste material of varieties in saline-alkali areas.
9. the fermentation process of claim 8, the varieties in saline-alkali areas is temperate zone semiarid zone.
10. fertilizer prepared by claim 5-9 fermentation process is used for the purposes that areal plant plait is cultivated.
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| CN105420158A (en) * | 2015-12-21 | 2016-03-23 | 镇江兴农有机肥有限公司 | Special fermentation microbial inoculum for harmless treatment of solid organic waste |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101186879B (en) * | 2007-12-05 | 2010-07-14 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial agent |
| CN102219570A (en) * | 2011-05-09 | 2011-10-19 | 成都市泰兴生态农业有限公司 | Bio-organic fertilizer produced by using livestock manure |
| CN102219570B (en) * | 2011-05-09 | 2012-12-26 | 成都市泰兴生态农业有限公司 | Bio-organic fertilizer produced by using livestock manure |
| CN105420158A (en) * | 2015-12-21 | 2016-03-23 | 镇江兴农有机肥有限公司 | Special fermentation microbial inoculum for harmless treatment of solid organic waste |
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