CN107002066A - 复合式多步核酸扩增 - Google Patents

复合式多步核酸扩增 Download PDF

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Publication number
CN107002066A
CN107002066A CN201580061362.4A CN201580061362A CN107002066A CN 107002066 A CN107002066 A CN 107002066A CN 201580061362 A CN201580061362 A CN 201580061362A CN 107002066 A CN107002066 A CN 107002066A
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CN
China
Prior art keywords
nucleic acid
primer
sample
amplification
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580061362.4A
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English (en)
Chinese (zh)
Inventor
P·帕特尔
I·吴
A·理查德森
K·贝尔霍钦
J·李
S·塔巴克曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Serranos Intellectual Property LLC
Original Assignee
Theranos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2014/056151 external-priority patent/WO2015076919A1/en
Application filed by Theranos Inc filed Critical Theranos Inc
Publication of CN107002066A publication Critical patent/CN107002066A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201580061362.4A 2014-09-17 2015-09-17 复合式多步核酸扩增 Pending CN107002066A (zh)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201462051945P 2014-09-17 2014-09-17
US201462051912P 2014-09-17 2014-09-17
USPCT/US2014/056151 2014-09-17
US62/051,945 2014-09-17
US62/051,912 2014-09-17
PCT/US2014/056151 WO2015076919A1 (en) 2013-11-22 2014-09-17 Nucleic Acid Amplification
US201462068605P 2014-10-24 2014-10-24
US201462068603P 2014-10-24 2014-10-24
US62/068,603 2014-10-24
US62/068,605 2014-10-24
US201562151358P 2015-04-22 2015-04-22
US62/151,358 2015-04-22
PCT/US2015/050822 WO2016044673A1 (en) 2014-09-17 2015-09-17 Hybrid multi-step nucleic acid amplification

Publications (1)

Publication Number Publication Date
CN107002066A true CN107002066A (zh) 2017-08-01

Family

ID=55533878

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201580061362.4A Pending CN107002066A (zh) 2014-09-17 2015-09-17 复合式多步核酸扩增
CN202110943909.0A Pending CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物
CN201580062485.XA Active CN108026591B (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN202110943909.0A Pending CN113736863A (zh) 2014-09-17 2015-09-17 诊断方法和组合物
CN201580062485.XA Active CN108026591B (zh) 2014-09-17 2015-09-17 诊断方法和组合物

Country Status (8)

Country Link
US (3) US9909193B2 (enExample)
EP (2) EP3194590A4 (enExample)
JP (6) JP2017531424A (enExample)
CN (3) CN107002066A (enExample)
BR (1) BR112017005392A2 (enExample)
CA (2) CA2961209A1 (enExample)
MX (2) MX2017003473A (enExample)
WO (2) WO2016044664A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017531424A (ja) 2014-09-17 2017-10-26 セラノス， インコーポレイテッドＴｈｅｒａｎｏｓ， Ｉｎｃ． ハイブリッド多元ステップ核酸増幅
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
EP3555293A4 (en) 2016-12-15 2020-08-12 Meharry Medical College ANTIVIRAL AGENTS
FI130673B1 (en) * 2020-11-24 2024-01-11 Mobidiag Oy Method and system for performing a cumulative nucleic acid amplification reaction

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US6605451B1 (en) * 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US20090263789A1 (en) * 1998-06-24 2009-10-22 Enzo Life Sciences, Inc. Method for Detecting Nucleic Acid Sequences
US20130224746A1 (en) * 1996-05-29 2013-08-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

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US7041447B2 (en) * 2001-04-09 2006-05-09 St. Jude Children's Hospital, Inc. Haplotyping method for multiple distal nucleotide polymorphisms
EP1319718A1 (en) * 2001-12-14 2003-06-18 Keygene N.V. High throughput analysis and detection of multiple target sequences
WO2005014810A1 (ja) * 2003-08-08 2005-02-17 National Institute Of Advanced Industrial Science And Technology ダンベル型ｄｎａの効率的な製造方法
JP4379087B2 (ja) * 2003-11-07 2009-12-09 トヨタ自動車株式会社 ゲラニルゲラニル二リン酸合成酵素及びゲラニルゲラニオールの製造方法
US20100075384A1 (en) * 2004-03-25 2010-03-25 New England Biolabs, Inc. Helicase-dependent amplification of circular nucleic acids
JP4644685B2 (ja) * 2004-07-02 2011-03-02 株式会社ダナフォーム 塩基配列タグの調製方法
EP2458011B1 (en) * 2005-10-03 2013-07-24 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
CA2691360A1 (en) * 2007-06-22 2008-12-31 Stephen A. Brown Specific amplification of tumor specific dna sequences
CA3170924A1 (en) 2007-10-02 2009-04-09 Labrador Diagnostics Llc Modular point-of-care devices and uses thereof
US20120115140A1 (en) * 2009-04-29 2012-05-10 Js Genetics Inc. Molecular Diagnosis of Fragile X Syndrome Associated with FMR1 Gene
IT1394539B1 (it) * 2009-05-19 2012-07-05 Sentinel Ch S P A Uso di uno stabilizzante delle polimerasi per la liofilizzazione e la preparazione di kit pronti per l'uso
WO2012038049A2 (en) * 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
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Also Published As

Publication number Publication date
JP2022110152A (ja) 2022-07-28
JP2023011943A (ja) 2023-01-24
EP3194629A1 (en) 2017-07-26
US20170342476A1 (en) 2017-11-30
US9909193B2 (en) 2018-03-06
JP2017531424A (ja) 2017-10-26
WO2016044664A1 (en) 2016-03-24
JP2021000138A (ja) 2021-01-07
CA2960859A1 (en) 2016-03-24
CA2961209A1 (en) 2016-03-24
JP2021006056A (ja) 2021-01-21
WO2016044673A1 (en) 2016-03-24
EP3194629A4 (en) 2018-03-07
MX2017003473A (es) 2017-08-02
CN113736863A (zh) 2021-12-03
BR112017005392A2 (pt) 2017-12-12
US20160201148A1 (en) 2016-07-14
MX366113B (es) 2019-06-26
CN108026591B (zh) 2021-09-07
CN108026591A (zh) 2018-05-11
US20180223381A1 (en) 2018-08-09
EP3194590A1 (en) 2017-07-26
JP2017532030A (ja) 2017-11-02
MX2017003475A (es) 2017-08-02
EP3194590A4 (en) 2018-05-09

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