CN107001431B - 基于细菌的蛋白质递送 - Google Patents

基于细菌的蛋白质递送 Download PDF

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CN107001431B
CN107001431B CN201580040391.2A CN201580040391A CN107001431B CN 107001431 B CN107001431 B CN 107001431B CN 201580040391 A CN201580040391 A CN 201580040391A CN 107001431 B CN107001431 B CN 107001431B
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CN107001431A (zh
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塞西尔·阿里厄梅卢
西蒙·伊泰格
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Universitaet Basel
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/255—Salmonella (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201580040391.2A 2014-05-21 2015-05-20 基于细菌的蛋白质递送 Active CN107001431B (zh)

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CN202211325325.8A CN115960919A (zh) 2014-05-21 2015-05-20 基于细菌的蛋白质递送

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EP14169335.8 2014-05-21
EP14169335 2014-05-21
PCT/EP2015/061086 WO2015177197A1 (en) 2014-05-21 2015-05-20 Bacteria-based protein delivery

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HUE046025T2 (hu) 2014-05-21 2020-01-28 Univ Basel Baktérium-alapú fehérjeszállítás
PT3377634T (pt) 2015-11-19 2020-11-16 Univ Basel Distribuição de proteínas baseada em bactérias
PT3377094T (pt) 2015-11-19 2021-07-08 Univ Basel Bactérias com virulência atenuada para o tratamento de tumores sólidos malignos
US11365225B2 (en) * 2015-11-19 2022-06-21 President And Fellows Of Harvard College Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
US12351805B2 (en) 2015-11-19 2025-07-08 Northeastern University Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
SMT202100537T1 (it) * 2016-12-20 2021-11-12 Univ Basel Veicolazione di proteine basata su batteri con virulenza attenuata
WO2018183850A2 (en) * 2017-03-31 2018-10-04 Arizona Board Of Regents On Behalf Of The University Of Arizona Methods, systems, and compositions for inhibiting virulance of a/e family pathogens
CN107858367A (zh) * 2017-12-05 2018-03-30 复旦大学附属肿瘤医院 一种利用细菌靶向投递治疗性蛋白的系统及其应用
WO2020223601A1 (en) * 2019-05-01 2020-11-05 Innate Biologics Llc Immunomodulatory compositions and methods
EP4045672A4 (en) * 2019-10-18 2023-11-22 University of Virginia Patent Foundation COMPOSITIONS AND METHODS FOR PRODUCING ENHANCED IMMUNE RESPONSES AND RAPID ANTIBODY PRODUCTION
CN111848803B (zh) * 2020-07-21 2022-04-08 珠海中科先进技术研究院有限公司 一种具有突出酸碱稳定性的磷脂酰肌醇蛋白聚糖3的纳米抗体及其制备方法
WO2022087856A1 (zh) * 2020-10-26 2022-05-05 南京吉芮康生物科技研究院有限公司 一种减毒沙门氏菌分泌表达rbd结构域蛋白的新型冠状病毒疫苗抗原递呈系统及其应用
CN114712496B (zh) * 2022-04-29 2023-10-13 中山大学·深圳 一种展示新抗原的细菌衍生外膜囊泡疫苗及制备方法和在制备癌症免疫治疗试剂盒中的应用
CN115896314A (zh) * 2022-07-14 2023-04-04 四川大学 一种基于lf-rpa的猕猴桃细菌性溃疡病菌的无设备、可视化检测方法
CN117511912B (zh) * 2023-12-22 2024-03-29 辉大(上海)生物科技有限公司 IscB多肽、包含其的系统及其用途

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CN1304442A (zh) * 1998-03-06 2001-07-18 皮埃尔B·范·德·布鲁根 用重组耶尔森氏菌将蛋白质送递到真核生物细胞中
WO2008019183A2 (en) * 2006-05-18 2008-02-14 The Regents Of The University Of California Biopolymer and protein production using type iii secretion systems of gram negative bacteria
WO2009115531A2 (en) * 2008-03-17 2009-09-24 Universitätsklinikum Münster Yopm as delivery vehicle for cargo molecules and as biological therapeutic for immunomodulation of inflammatory reactions

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GB2483595B (en) * 2009-05-22 2017-03-29 The Arizona Board Of Regents For And On Behalf Of Arizona State Univ Recombinant bacterium and methods of antigen and nucleic acid delivery
JP6936934B2 (ja) 2013-09-24 2021-09-22 メディシナ セラピューティクス インコーポレイテッド インターロイキン−４受容体結合融合タンパク質及びその使用
US10143743B2 (en) 2013-11-18 2018-12-04 Yale University Non-replicating bacterial nanoparticle delivery system and methods of use
HUE046025T2 (hu) 2014-05-21 2020-01-28 Univ Basel Baktérium-alapú fehérjeszállítás
PT3377634T (pt) 2015-11-19 2020-11-16 Univ Basel Distribuição de proteínas baseada em bactérias
PT3377094T (pt) 2015-11-19 2021-07-08 Univ Basel Bactérias com virulência atenuada para o tratamento de tumores sólidos malignos
SMT202100537T1 (it) 2016-12-20 2021-11-12 Univ Basel Veicolazione di proteine basata su batteri con virulenza attenuata

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1304442A (zh) * 1998-03-06 2001-07-18 皮埃尔B·范·德·布鲁根 用重组耶尔森氏菌将蛋白质送递到真核生物细胞中
WO2008019183A2 (en) * 2006-05-18 2008-02-14 The Regents Of The University Of California Biopolymer and protein production using type iii secretion systems of gram negative bacteria
WO2009115531A2 (en) * 2008-03-17 2009-09-24 Universitätsklinikum Münster Yopm as delivery vehicle for cargo molecules and as biological therapeutic for immunomodulation of inflammatory reactions

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BR112016027196A2 (pt) 2018-01-30
CA2948570A1 (en) 2015-11-26
KR20220127345A (ko) 2022-09-19
US20240401065A1 (en) 2024-12-05
KR102648293B1 (ko) 2024-03-15
US12091670B2 (en) 2024-09-17
CN107001431A (zh) 2017-08-01
KR20170010803A (ko) 2017-02-01
EP3660034A1 (en) 2020-06-03
IL283280A (en) 2021-07-29
US10889823B2 (en) 2021-01-12
DK3145946T3 (da) 2019-11-18
HUE046025T2 (hu) 2020-01-28
JP7509821B2 (ja) 2024-07-02
US20170198297A1 (en) 2017-07-13
SMT201900626T1 (it) 2020-01-14
KR102440293B1 (ko) 2022-09-05
JP2022109967A (ja) 2022-07-28
BR112016027196B1 (pt) 2023-05-02
IL283280B1 (en) 2023-04-01
EA201692025A1 (ru) 2017-05-31
IL283280B2 (en) 2023-08-01
RS59583B1 (sr) 2019-12-31
PL3145946T3 (pl) 2020-02-28
ES2754508T3 (es) 2020-04-17
HRP20192050T1 (hr) 2020-02-07
CN115960919A (zh) 2023-04-14
SG11201609629YA (en) 2016-12-29
PT3145946T (pt) 2019-11-20
AU2015261905B2 (en) 2020-07-09
IL248827B (en) 2021-07-29
JP2020182478A (ja) 2020-11-12
JP6797025B2 (ja) 2020-12-09
EP3145946A1 (en) 2017-03-29
AU2015261905A1 (en) 2016-12-01
LT3145946T (lt) 2019-11-25
SI3145946T1 (sl) 2019-12-31
CA2948570C (en) 2023-09-12
US20210155942A1 (en) 2021-05-27
JP2017517257A (ja) 2017-06-29
EP3145946B1 (en) 2019-08-14
CY1122701T1 (el) 2021-03-12
WO2015177197A1 (en) 2015-11-26
IL248827A0 (en) 2017-01-31

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