CN106995826A - A kind of fermentation medium for being used to produce S abscisic acids - Google Patents
A kind of fermentation medium for being used to produce S abscisic acids Download PDFInfo
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- CN106995826A CN106995826A CN201710253782.3A CN201710253782A CN106995826A CN 106995826 A CN106995826 A CN 106995826A CN 201710253782 A CN201710253782 A CN 201710253782A CN 106995826 A CN106995826 A CN 106995826A
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- CN
- China
- Prior art keywords
- fermentation medium
- abscisic acids
- abscisic
- oil
- organic nitrogen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The present invention discloses a kind of fermentation medium for being used to produce S abscisic acids, and its active ingredient includes by weight percentage：Carbon source 10% 20%, organic nitrogen source 3% 16%, inorganic phosphate 0.5% 4%, polyalcohol 0.1% 2%；One or more of the carbon source in sucrose, glucose, starch, lactic acid or mannitol；One or more of the organic nitrogen source in corn flour, soybean protein, groundnut meal, cottonseed meal, corn steep liquor, urea or sweet casein；One or more of the polyalcohol in dihydric alcohol or trihydroxylic alcohol.The S abscisic acids produced using the fermentation medium have stronger physiologically active, with low cost, the features such as shortening fermentation period, raising S abscisic acid yields.
Description
Technical field
The present invention relates to plant growth regulator field, and in particular to a kind of fermented and cultured for being used to produce S- abscisic acids
Base.
Background technology
S- abscisic acid original name natural abscisic acids, are the pure natural products that all green plantss contain, and to photaesthesia, category is strong
Light decomposition of chemical compounds.Abscisic acid is also a kind of important plant hormone simultaneously.It rises many in the growing of plant
Effect, is agriculturally also having broad application prospects.It can promote the germination of seed using the abscisic acid seed soaking of low concentration；Use
The abscisic acid seed soaking of high concentration can then suppress germination, be widely used in terms of seed storage；While abscisic acid is also
The winter resistance, drought resistance and salt tolerance of crop can be strengthened.Therefore, abscisic acid serves vital work in terms of plant growth
With.
At present, the preparation method of S- abscisic acids has three kinds.One kind is directly extracts from plant, although it is in plant
It is widely distributed, but its content is very low, it was reported that 9mg S- abscisic acid crystal is just extracted from 200kg cotton bolls, thus it is difficult
To be obtained using direct extraction method；One kind is to prepare S- abscisic acids using chemical synthesis, and synthesized racemic form S- lures anti-
Element is the mixture of natural type and non-natural type abscisic acid, not only expensive, and the physiologically active of synthesis is low；One kind is micro-
Biofermentation produces abscisic acid, and this method flows during the fermentation plus inositol releases the technique of glucose repression to prepare natural take off
Fall acid, so that the yield of abscisic acid is improved, although this method improves the fermentation yield of abscisic acid, but hair to a certain extent
Ferment technique is cumbersome, and the accessory substance produced that ferments is more, to the inadequate composition of utilization of oxygen, adds cost, is unsuitable for large-scale
Production.
The content of the invention
In view of this, the present invention provide it is a kind of be used to produce the fermentation mediums of S- abscisic acids, with technological process it is simple,
The advantages of reducing production cost, improve the yield of S- abscisic acids.
To solve above technical problem, this application provides a kind of fermentation medium for being used to produce S- abscisic acids, it has
Effect composition includes by weight percentage：Carbon source 10%-20%, organic nitrogen source 3%-16%, inorganic phosphate 0.5%-4%,
Polyalcohol 0.1%-2%；One or more of the carbon source in sucrose, glucose, starch, lactic acid or mannitol；It is described
The one kind or many of organic nitrogen source in corn flour, soybean protein, groundnut meal, cottonseed meal, corn steep liquor, urea or sweet casein
Kind；One or more of the polyalcohol in dihydric alcohol or trihydroxylic alcohol.
It is preferred that, the active ingredient includes by weight percentage：Carbon source 15%-20%；Organic nitrogen source 8%-16%；
Inorganic phosphate 2%-4%.
It is preferred that, the active ingredient includes by weight percentage：Carbon source 15%-18%；Organic nitrogen source 8%-12%；
Inorganic phosphate 2%-3%.
It is preferred that, one or more of the carbon source in sucrose, starch or glucose；The organic nitrogen source is selected from jade
One or more in ground rice, urea, casein, groundnut meal or soybean protein；The polyalcohol is selected from polypropylene glycol, poly- carbon
One or more in acid esters dihydric alcohol, polyether Glycols, glycerine or trihydroxy methyl propane.
It is preferred that, the carbon source is selected from sucrose or glucose；The organic nitrogen source is selected from corn flour, groundnut meal, urea
Or soybean protein；One or more of the polyalcohol in glycerine, polypropylene glycol or trihydroxy methyl propane.
It is preferred that, the fermentation medium active ingredient also includes natural oil 1%-2% by weight percentage；Institute
State one or more of the natural oil in salad oil, rapeseed oil, peanut oil, cottonseed oil or palm-kernel oil.
It is preferred that, one or more of the natural oil in salad oil, peanut oil or rapeseed oil.
It is preferred that, the fermentation medium also includes element sulphur, and the fermentation medium active ingredient is also included by weight
The element sulphur 1%-2% of percentages.
It is preferred that, trace element is also included in the fermentation medium.
It is preferred that, the trace element is selected from ferro element, Zn-ef ficiency, manganese element, copper, bromo element, I, silicon member
One or more in element, cobalt element, boron element, arsenic element or molybdenum element.
It is preferred that, the trace element is manganese element, ferro element, Zn-ef ficiency, cobalt element, molybdenum element, arsenic element or boron are first
One or more in element.
It is preferred that, the pH value of the fermentation medium is in the range of 4.0-7.0.
It is preferred that, the pH value of the fermentation medium is in the range of 4.0-6.0.
First, organic nitrogen source is selected from corn flour, soybean protein or groundnut meal in fermentation medium, is because this 3 kinds are sought
Support in material containing nutrition compositions such as more biotins, can effectively improve the yield of S- abscisic acids.Secondly, S- abscisic acids
For the secondary metabolite of fungi, carbon source is added into culture medium, such as starch, sucrose or glucose can promote fungi
Metabolism, so as to promote its growth.Finally, natural oil, such as salad oil, rapeseed oil, flower are added in the fermentation medium
Oil generation, cottonseed oil or palm-kernel oil etc., can promote thalline oil-producing and producing enzyme to some extent.
Following methods and condition specifically preferred according to the invention：Trained after strain Botrytis cinerea is activated in seed culture medium
Support, obtain seed culture fluid.Seed culture fluid is moved into fermentation medium and cultivated, wherein the active ingredient of fermentation medium
For：Soybean protein, sucrose and glucose are with 1:1 mass mixing, potassium dihydrogen phosphate, magnesium sulfate, glycerine, calcium propionate, sodium acetate
And manganese sulfate, the content ratio of above-mentioned active ingredient is 3.6：10：0.6：0.6：2：0.5：0.5：0.01.Wherein seed culture
The time that liquid is cultivated in the fermentation medium is 18 days, and cultivation temperature is 26.5 DEG C.
The commercially available gained of reagent and raw material used in the present invention.
The positive achievement that the present invention is obtained is：
The shorter S- abscisic acid fermentation periods of gained are 18 days, and 80% is improved compared with prior art fermentation time；While S-
The yield of abscisic acid also brings up to 8000ppm, and 106% is improved compared with prior art.
So far, the fermentation medium prepared using this method be used to producing S- abscisic acids can reach technological process it is simple,
Prepare the features such as S- abscisic acids that are with low cost, preparing are with stronger physiologically active.
Embodiment
In order that those skilled in the art more fully understands technical scheme, with reference to embodiment
The present invention is described in further detail.
Drought resistance of the abscisic acid to plant：During water deficit, the accumulation of abscisic acid and the enhancing of drought-resistant ability in plant
In notable positive correlation.Abscisic acid, which has, to be promoted stomata to close and prevents stomatal opening, is reduced moisture transpiration, is improved leaf water potential,
Mitigate the physiological effect of Water deficit levels.
Frost resistance of the abscisic acid to plant：To the certain density abscisic acid of seedling foliage-spray, it is possible to decrease in plant body
Relative conductivity and mda content, delay the degraded of chlorophyll, increase the accumulation of proline, reduce blade wilting area and dead
Rate is died, Chilling injury index is effectively reduced, the cold resistance of seedling is improved.
Resistivity of the abscisic acid to high temperature：High temperature stress can produce influence to some physical signs of plant, in high temperature (43
± 0.50 DEG C) stress under, the superoxide dismutase activity in plant leaf blade is substantially reduced, and mda content substantially increases, can
Soluble proteins, soluble sugar, proline, ascorbic acid and reduced glutathione content are all substantially reduced.Exogenous Abscisic Acid
High temperature stress can effectively be slowed down to the influence in plant leaf blade about physiological and biochemical index, so as to effectively improve Genes For Plant Tolerance hyperthermal injury
Harmful ability.
However, the abscisic acid that conventional method is prepared has, physiologically active is low, technique is cumbersome, production cost is more low lacks
Point.So needing a kind of feasible method to be used for preparing abscisic acid.
The application embodiment is prepared using preparation method, a kind of fermentation medium for being used to produce S- abscisic acids
Its active ingredient includes by weight percentage：Carbon source 10%-20%, organic nitrogen source 3%-16%, inorganic phosphate 0.5%-
4%th, polyalcohol 0.1%-2%；One or more of the carbon source in sucrose, glucose, starch, lactic acid or mannitol；
The one kind of the organic nitrogen source in corn flour, soybean protein, groundnut meal, cottonseed meal, corn steep liquor, urea or sweet casein
Or it is a variety of；One or more of the polyalcohol in dihydric alcohol or trihydroxylic alcohol.
Wherein, active ingredient preferably includes by weight percentage：Carbon source 15%-20%；Organic nitrogen source 8%-16%；
Inorganic phosphate 2%-4%.Preferred active ingredient includes by weight percentage：Carbon source 15%-18%, organic nitrogen source
8%-12%, inorganic phosphate 2%-3%.Wherein, one or more of the carbon source in sucrose, starch or glucose；
One or more of the organic nitrogen source in corn flour, urea, casein, groundnut meal or soybean protein；It is described polynary
One or more of the alcohol in polypropylene glycol, PCDL, polyether Glycols, glycerine or trihydroxy methyl propane,
Preferred one or more of the polyalcohol in glycerine, polypropylene glycol or trihydroxy methyl propane.Add in the medium
Carbon source is conducive to strain to accumulate metabolite；The yield of S- abscisic acids can be increased by adding organic nitrogen source；Adding polyalcohol has
The effect of moisturizing moisturizing.
Also include in a kind of fermentation medium for producing S- abscisic acids in trace element, natural oil or element sulphur
It is one or more；It includes by weight percentage：Micro- 0.05%-1%, natural oil 1%-2%, element sulphur 1%-
2%, wherein, the trace element preferably is selected from ferro element, Zn-ef ficiency, manganese element, copper, bromo element, I, silicon member
One or more in element, cobalt element, boron element, arsenic element or molybdenum element, the preferred trace element selected from manganese element,
One or more in ferro element, Zn-ef ficiency, cobalt element, molybdenum element, arsenic element or boron element；It is preferred that the natural oil choosing
One or more from salad oil, rapeseed oil, peanut oil, cottonseed oil or palm-kernel oil, the preferred natural oil choosing
One or more from salad oil, peanut oil or rapeseed oil.The purpose for adding natural oil in the medium is that it can not
With the promotion thalline producing enzyme and oil-producing of degree.
A kind of fermentation medium for being used to produce S- abscisic acids, the pH of its culture medium is in the range of 4.0-7.0, wherein excellent
The pH of the fermentation medium of choosing is in 4.0-6.0.Alkaline conditioner is automatically replenished when pH value is less than 4.0 in fermentation medium；
PH value is automatically replenished fermentation medium when being higher than 7.0.PH fermentation times in the range of 4.0-6.0 are most short, and resulting S- is lured
Anti- plain activity is higher.
Present invention also offers a kind of method for producing S- abscisic acids, it is characterised in that including：Seed culture fluid is accessed
Cultivated into the fermentation medium of the present invention.
It is below the specific embodiment of the invention, elaborates concrete scheme of the present invention.
Embodiment one：
Table 1 is the formula of the fermentation medium of embodiment 1
S- abscisic acids, the abscisic acid content of 18 days gained of fermentation time are produced according to the formula of above-mentioned fermentation medium
5780ppm。
Embodiment two：
Table 2 is the formula of the fermentation medium of embodiment 2
| Material name | Ratio (percentage by weight) |
| Sucrose | 20 |
| Corn flour | 15 |
| Potassium dihydrogen phosphate | 0.6 |
| Sodium acetate | 2 |
| Epsom salt | 0.6 |
| Manganese sulfate monohydrate | 0.01 |
| Calcium propionate | 0.01 |
S- abscisic acids, the abscisic acid content of 21 days gained of fermentation time are produced according to the formula of above-mentioned fermentation medium
6510ppm。
Embodiment three：
Carbon source in culture medium is glucose and the mixture of sucrose, and its mass ratio is 1:1.
Table 3 is the formula of the fermentation medium of embodiment 3
| Material name | Ratio (percentage by weight) |
| Glucose, sucrose | 10:10 |
| Corn flour | 15 |
| Potassium dihydrogen phosphate | 0.6 |
| Calcium propionate | 1 |
| Epsom salt | 0.6 |
| Manganese sulfate monohydrate | 0.01 |
| Sodium acetate | 0.6 |
S- abscisic acids, the abscisic acid content of 19 days gained of fermentation time are produced according to the formula of above-mentioned fermentation medium
7864ppm。
Example IV：
Table 4 is the formula of the fermentation medium of embodiment 4
| Material name | Ratio (percentage by weight) |
| Glucose | 10 |
| Soybean protein | 3.6 |
| Potassium dihydrogen phosphate | 0.6 |
| Calcium propionate | 0.01 |
| Epsom salt | 0.6 |
| Manganese sulfate monohydrate | 0.01 |
| Sodium acetate | 1 |
S- abscisic acids, the abscisic acid content of 18 days gained of fermentation time are produced according to the formula of above-mentioned fermentation medium
6075ppm。
Embodiment five：
Table 5 is the formula of the fermentation medium of embodiment 5
| Material name | Ratio (percentage by weight) |
| Sucrose | 20 |
| Soybean protein | 3.6 |
| Potassium dihydrogen phosphate | 0.6 |
| Calcium propionate | 0.5 |
| Epsom salt | 0.6 |
| Manganese sulfate monohydrate | 0.01 |
| Sodium acetate | 0.5 |
S- abscisic acids, the abscisic acid content of 22 days gained of fermentation time are produced according to the formula of above-mentioned fermentation medium
7608ppm。
Embodiment six：
Table 6 is the formula of the fermentation medium of embodiment 6
S- abscisic acids, the S- abscisic acid contents of 18 days gained of fermentation time are produced according to the formula of above-mentioned fermentation medium
8264ppm。
It can be drawn according to above-described embodiment, lure anti-using the formula production S- of fermentation medium described in embodiment six
Element, its fermentation time is most short, and the yield highest of resulting S- abscisic acids.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be defined by claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made, these change
Enter and retouch and also should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of fermentation medium for being used to produce S- abscisic acids, it is characterised in that active ingredient includes by weight percentage：
Carbon source 10%-20%, organic nitrogen source 3%-16%, inorganic phosphate 0.5%-4%, polyalcohol 0.1%-2%；The carbon
One or more of the source in sucrose, glucose, starch, lactic acid or mannitol；The organic nitrogen source is selected from corn flour, soybean
One or more in albumen, groundnut meal, cottonseed meal, corn steep liquor, urea or sweet casein；The polyalcohol is selected from dihydric alcohol
Or the one or more in trihydroxylic alcohol.
2. it is used for the fermentation medium for producing S- abscisic acids according to claim 1, it is characterised in that the active ingredient
Include by weight percentage：Carbon source 15%-20%, organic nitrogen source 8%-16%, inorganic phosphate 2%-4%.
3. it is used for the fermentation medium for producing S- abscisic acids according to claim 1, it is characterised in that the carbon source is selected from sugarcane
One or more in sugar, starch or glucose；The organic nitrogen source is selected from corn flour, groundnut meal, urea, casein or big
One or more in legumin；The polyalcohol be selected from polypropylene glycol, PCDL, polyether Glycols, glycerine or
One or more in trihydroxy methyl propane.
4. the fermentation medium of S- abscisic acids is produced according to claim 1, it is characterised in that the fermentation medium has
Composition is imitated also including natural oil 1%-2% by weight percentage；The natural oil is selected from salad oil, rapeseed oil, flower
One or more in oil generation, cottonseed oil or palm-kernel oil.
5. the fermentation medium of S- abscisic acids is produced according to claim 1, it is characterised in that the fermentation medium has
Composition is imitated also including element sulphur 1%-2% by weight percentage.
6. the fermentation medium of S- abscisic acids is produced according to claim 1, it is characterised in that in the fermentation medium
Also include trace element.
7. the fermentation medium of S- abscisic acids is produced according to claim 6, it is characterised in that the trace element is iron
In element, Zn-ef ficiency, manganese element, copper, bromo element, I, element silicon, cobalt element, boron element, arsenic element or molybdenum element
One or more.
8. the fermentation medium of S- abscisic acids is produced according to claim 7, it is characterised in that the trace element is manganese
One or more in element, ferro element, Zn-ef ficiency, cobalt element, molybdenum element, arsenic element or boron element.
9. the fermentation medium of the production S- abscisic acids according to claim 1, it is characterised in that the fermentation medium
PH value in the range of 4.0-7.0.
10. the fermentation medium of the production S- abscisic acids according to claim 8, it is characterised in that the fermented and cultured
The pH value of base is in the range of 4.0-6.0.
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| Application Number | Priority Date | Filing Date | Title |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103409474A (en) * | 2013-07-10 | 2013-11-27 | 四川科瑞森生物工程有限公司 | Method for producing abscisic acid by solid state fermentation of fungi |
| CN104988188A (en) * | 2015-07-20 | 2015-10-21 | 中国药科大学 | Method for increasing fermentation output of abscisic acid |
-
2017
- 2017-04-18 CN CN201710253782.3A patent/CN106995826A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103409474A (en) * | 2013-07-10 | 2013-11-27 | 四川科瑞森生物工程有限公司 | Method for producing abscisic acid by solid state fermentation of fungi |
| CN104988188A (en) * | 2015-07-20 | 2015-10-21 | 中国药科大学 | Method for increasing fermentation output of abscisic acid |
Non-Patent Citations (3)
| Title |
|---|
| 施天穹: "微生物发酵法产脱落酸的研究进展", 《化工进展》 * |
| 郑珩: "不同营养物质对脱落酸液体发酵产量的影响", 《氨基酸和生物资源》 * |
| 郑珩: "脱落酸产生菌液体发酵培养基的优化", 《中国药科大学学报》 * |
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