CN106977579B - 一种从积雪草中提取羟基积雪草甙的工艺 - Google Patents
一种从积雪草中提取羟基积雪草甙的工艺 Download PDFInfo
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- CN106977579B CN106977579B CN201710294529.2A CN201710294529A CN106977579B CN 106977579 B CN106977579 B CN 106977579B CN 201710294529 A CN201710294529 A CN 201710294529A CN 106977579 B CN106977579 B CN 106977579B
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- madecassoside
- centella
- extracting
- technique according
- absorbent resin
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- QCYLIQBVLZBPNK-UHFFFAOYSA-N asiaticoside A Natural products O1C(C(=O)C(C)C)=CC(C)C(C2(C(OC(C)=O)CC34C5)C)C1CC2(C)C3CCC(C1(C)C)C45CCC1OC1OCC(O)C(O)C1O QCYLIQBVLZBPNK-UHFFFAOYSA-N 0.000 title claims abstract description 68
- BNMGUJRJUUDLHW-HCZMHFOYSA-N Madecassoside Chemical compound O([C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)O)OC[C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)[C@]12CC[C@H]([C@@H]([C@H]1C=1[C@@]([C@@]3(C[C@@H](O)[C@H]4[C@](C)(CO)[C@@H](O)[C@H](O)C[C@]4(C)[C@H]3CC=1)C)(C)CC2)C)C)[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O BNMGUJRJUUDLHW-HCZMHFOYSA-N 0.000 title claims abstract description 65
- BNMGUJRJUUDLHW-HLUHVYOBSA-N Madecassoside Natural products C[C@@H]1CC[C@@]2(CC[C@]3(C)C(=CC[C@@H]4[C@@]5(C)C[C@@H](O)[C@H](O)[C@@](C)(CO)[C@@H]5[C@H](O)C[C@@]34C)[C@@H]2[C@H]1C)C(=O)O[C@@H]6O[C@H](CO[C@@H]7O[C@H](CO)[C@@H](O[C@@H]8O[C@H](C)[C@H](O)[C@@H](O)[C@H]8O)[C@H](O)[C@H]7O)[C@@H](O)[C@H](O)[C@H]6O BNMGUJRJUUDLHW-HLUHVYOBSA-N 0.000 title claims abstract description 65
- 229940090813 madecassoside Drugs 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 39
- 244000146462 Centella asiatica Species 0.000 title claims description 15
- 235000004032 Centella asiatica Nutrition 0.000 title claims description 15
- 241000167550 Centella Species 0.000 claims abstract description 55
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 50
- 239000007788 liquid Substances 0.000 claims description 31
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 30
- 238000004042 decolorization Methods 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000000605 extraction Methods 0.000 claims description 25
- 235000019441 ethanol Nutrition 0.000 claims description 22
- 239000011347 resin Substances 0.000 claims description 22
- 229920005989 resin Polymers 0.000 claims description 22
- 239000002250 absorbent Substances 0.000 claims description 21
- 230000002745 absorbent Effects 0.000 claims description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 16
- 229910052799 carbon Inorganic materials 0.000 claims description 16
- 239000012535 impurity Substances 0.000 claims description 16
- 239000013078 crystal Substances 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 14
- 244000144980 herd Species 0.000 claims description 14
- 239000002904 solvent Substances 0.000 claims description 14
- 238000002425 crystallisation Methods 0.000 claims description 13
- 230000008025 crystallization Effects 0.000 claims description 13
- 239000006071 cream Substances 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 238000001179 sorption measurement Methods 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000012043 crude product Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000003795 desorption Methods 0.000 claims description 6
- 238000010992 reflux Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 4
- 239000013558 reference substance Substances 0.000 abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 229930182478 glucoside Natural products 0.000 description 6
- 150000008131 glucosides Chemical class 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 244000025254 Cannabis sativa Species 0.000 description 3
- WYQVAPGDARQUBT-FGWHUCSPSA-N Madecassol Chemical compound O([C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1O)O)OC[C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)[C@]12CC[C@H]([C@@H]([C@H]1C=1[C@@]([C@@]3(CC[C@H]4[C@](C)(CO)[C@@H](O)[C@H](O)C[C@]4(C)[C@H]3CC=1)C)(C)CC2)C)C)[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O WYQVAPGDARQUBT-FGWHUCSPSA-N 0.000 description 3
- 229940022757 asiaticoside Drugs 0.000 description 3
- WYQVAPGDARQUBT-XCWYDTOWSA-N asiaticoside Natural products O=C(O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@H]2[C@H](O)[C@H](O)[C@H](O[C@H]3[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O3)[C@@H](CO)O2)O1)[C@@]12[C@@H]([C@@H](C)[C@H](C)CC1)C=1[C@](C)([C@@]3(C)[C@@H]([C@@]4(C)[C@H]([C@@](CO)(C)[C@@H](O)[C@H](O)C4)CC3)CC=1)CC2 WYQVAPGDARQUBT-XCWYDTOWSA-N 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 239000003480 eluent Substances 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 150000003648 triterpenes Chemical class 0.000 description 3
- 239000000935 antidepressant agent Substances 0.000 description 2
- 229940005513 antidepressants Drugs 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000004451 qualitative analysis Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010023126 Jaundice Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000001430 anti-depressive effect Effects 0.000 description 1
- 239000012846 chemical reference substance Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 210000000867 larynx Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J63/00—Steroids in which the cyclopenta(a)hydrophenanthrene skeleton has been modified by expansion of only one ring by one or two atoms
- C07J63/008—Expansion of ring D by one atom, e.g. D homo steroids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Steroid Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (10)
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CN201710294529.2A CN106977579B (zh) | 2017-04-28 | 2017-04-28 | 一种从积雪草中提取羟基积雪草甙的工艺 |
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CN201710294529.2A CN106977579B (zh) | 2017-04-28 | 2017-04-28 | 一种从积雪草中提取羟基积雪草甙的工艺 |
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CN106977579A CN106977579A (zh) | 2017-07-25 |
CN106977579B true CN106977579B (zh) | 2019-05-07 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101948501A (zh) * | 2010-09-01 | 2011-01-19 | 桂林莱茵生物科技股份有限公司 | 一种羟基积雪草甙的制备方法 |
CN104592341A (zh) * | 2014-12-30 | 2015-05-06 | 广州白云山汉方现代药业有限公司 | 一种从积雪草中提取积雪草苷、羟基积雪草苷的方法 |
-
2017
- 2017-04-28 CN CN201710294529.2A patent/CN106977579B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101948501A (zh) * | 2010-09-01 | 2011-01-19 | 桂林莱茵生物科技股份有限公司 | 一种羟基积雪草甙的制备方法 |
CN104592341A (zh) * | 2014-12-30 | 2015-05-06 | 广州白云山汉方现代药业有限公司 | 一种从积雪草中提取积雪草苷、羟基积雪草苷的方法 |
Non-Patent Citations (1)
Title |
---|
积雪草苷的提取与分离;侯文砚;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20111215(第12期);第B016-177页 * |
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Effective date of registration: 20191022 Address after: 530199 Room 202, Building 1, Fengqi Oriental Building, Xiangshan Avenue, Wuming County, Nanning City, Guangxi Zhuang Autonomous Region Patentee after: Wuming Xin Yi Rong Niu vigorously Chinese herbal medicine planting professional cooperatives Address before: 530100 No. 22, Dingluo District, Biaoying Community, Wuming County, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: Nanning Xin Rong Rong Biological Technology Co., Ltd. |
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Effective date of registration: 20191212 Address after: 541004 room 415, University Science Park, Guilin National High tech Zone, No. 108, Qilidian Road, Qixing District, Guilin City, Guangxi Zhuang Autonomous Region Patentee after: Guilin taikrypton Biotechnology Co., Ltd Address before: 530199 Room 202, Building 1, Fengqi Oriental Building, Xiangshan Avenue, Wuming County, Nanning City, Guangxi Zhuang Autonomous Region Patentee before: Wuming Xin Yi Rong Niu vigorously Chinese herbal medicine planting professional cooperatives |
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